Restriction map for
pF1KA0551
(Circular) (Six-base) MAPSORT of: pF1KA0551.seq Check: 9927 from: 1 to: 7193
pF1KA0551 7193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 545 545
Size: 7193
AatII G_ACGT'C
Cuts at: 2833 3061 2833
Size: 228 6965
AccI GT'mk_AC
Cuts at: 1150 2481 3050 3540 4231 1150
Size: 1331 569 490 691 4112
Fragments arranged by size:
4112 1331 691 569 490
Acc65I G'GTAC_C
Cuts at: 4209 4209
Size: 7193
AclI AA'CG_TT
Cuts at: 7033 7033
Size: 7193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1600 1803 5018 5450 6197 6465 886
Size: 714 203 3215 432 747 268 1614
Fragments arranged by size:
3215 1614 747 714 432 268 203
AflIII A'CryG_T
Cuts at: 320 476 4545 5649 6626 320
Size: 156 4069 1104 977 887
Fragments arranged by size:
4069 1104 977 887 156
AgeI A'CCGG_T
Cuts at: 5581 5581
Size: 7193
AhdI GACnn_n'nnGTC
Cuts at: 756 3400 756
Size: 2644 4549
AleI CACnn'nnGTG
Cuts at: 3961 3961
Size: 7193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 522 522
Size: 7193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1978 2010 1978
Size: 32 7161
AlwNI CAG_nnn'CTG
Cuts at: 1224 1269 1503 2674 4498 6065 1224
Size: 45 234 1171 1824 1567 2352
Fragments arranged by size:
2352 1824 1567 1171 234 45
ApaLI G'TGCA_C
Cuts at: 1939 5963 1939
Size: 4024 3169
ApoI r'AATT_y
Cuts at: 889 2166 3574 3640 4170 4197 6546 6802 889
Size: 1277 1408 66 530 27 2349 256 1280
Fragments arranged by size:
2349 1408 1280 1277 530 256 66 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 7193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
AvaI C'yCGr_G
Cuts at: 4213 4213
Size: 7193
BamHI G'GATC_C
Cuts at: 2365 4218 2365
Size: 1853 5340
BanI G'GyrC_C
Cuts at: 693 2121 4209 4623 4871 4906 693
Size: 1428 2088 414 248 35 2980
Fragments arranged by size:
2980 2088 1428 414 248 35
BanII G_rGCy'C
Cuts at: 831 1121 2255 2269 2712 3624 4071 4207 5237
Size: 290 1134 14 443 912 447 136 1030
Cuts at: 5237 831
Size: 2787
Fragments arranged by size:
2787 1134 1030 912 447 443 290 136
14
BbeI G_GCGC'C
Cuts at: 4627 4875 4627
Size: 248 6945
BbsI GAAGACnn'nnnn_
Cuts at: 2111 2111
Size: 7193
BciVI GTATCCnnnnn_n'
Cuts at: 5091 5852 7193 5091
Size: 761 1341 5091
Fragments arranged by size:
5091 1341 761
BclI T'GATC_A
Cuts at: 455 3353 4713 455
Size: 2898 1360 2935
Fragments arranged by size:
2935 2898 1360
BfrBI ATG'CAT
Cuts at: 4059 4059
Size: 7193
BglII A'GATC_T
Cuts at: 147 1484 3666 4708 6745 147
Size: 1337 2182 1042 2037 595
Fragments arranged by size:
2182 2037 1337 1042 595
BlpI GC'TnA_GC
Cuts at: 635 4305 635
Size: 3670 3523
Bme1580I G_kGCm'C
Cuts at: 1943 4818 4911 5967 1943
Size: 2875 93 1056 3169
Fragments arranged by size:
3169 2875 1056 93
BmgBI CAC'GTC
Cuts at: 3959 3959
Size: 7193
BmrI ACTGGGnnnn_n'
Cuts at: 1926 2471 2956 4194 4405 4486 4821 6965 1926
Size: 545 485 1238 211 81 335 2144 2154
Fragments arranged by size:
2154 2144 1238 545 485 335 211 81
BmtI G_CTAG'C
Cuts at: 2492 2492
Size: 7193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1125 1140 2936 2997 5560 1125
Size: 15 1796 61 2563 2758
Fragments arranged by size:
2758 2563 1796 61 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 1342 2283 2289 2586 2696 2782 2855 2906
Size: 96 941 6 297 110 86 73 51
Cuts at: 2906 3655 4151 1246
Size: 749 496 4288
Fragments arranged by size:
4288 941 749 496 297 110 96 86
73 51 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 4369 5248 5740 6038 997
Size: 3372 879 492 298 2152
Fragments arranged by size:
3372 2152 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 971 2647 2758 6897 971
Size: 1676 111 4139 1267
Fragments arranged by size:
4139 1676 1267 111
BsaAI yAC'GTr
Cuts at: 4546 4546
Size: 7193
BsaBI GATnn'nnATC
Cuts at: 6 2513 4707 6744 6
Size: 2507 2194 2037 455
Fragments arranged by size:
2507 2194 2037 455
BsaHI Gr'CG_yC
Cuts at: 2830 3058 4624 4872 5574 7070 2830
Size: 228 1566 248 702 1496 2953
Fragments arranged by size:
2953 1566 1496 702 248 228
BsaWI w'CCGG_w
Cuts at: 344 2240 2549 3359 4019 4382 4903 5581 5855
Size: 1896 309 810 660 363 521 678 274
Cuts at: 5855 6002 6413 344
Size: 147 411 1124
Fragments arranged by size:
1896 1124 810 678 660 521 411 363
309 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1978 2008 2584 2614 2694 2724 1978
Size: 30 576 30 80 30 6447
Fragments arranged by size:
6447 576 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 1061 1111 1114 1264 1309 1339 1405 1423
Size: 559 50 3 150 45 30 66 18
Cuts at: 1423 1426 1431 1477 1480 2039 2586 2589 2623
Size: 3 5 46 3 559 547 3 34
Cuts at: 2623 2647 3815 502
Size: 24 1168 3880
Fragments arranged by size:
3880 1168 559 559 547 150 66 50
46 45 34 30 24 18 5 3
3 3 3
BseYI C'CCAG_C
Cuts at: 1120 2148 2321 2326 2345 3995 4618 5376 5953
Size: 1028 173 5 19 1650 623 758 577
Cuts at: 5953 6449 1120
Size: 496 1864
Fragments arranged by size:
1864 1650 1028 758 623 577 496 173
19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 2926 3284 523
Size: 2403 358 4432
Fragments arranged by size:
4432 2403 358
BsiEI CG_ry'CG
Cuts at: 104 4735 4781 5989 104
Size: 4631 46 1208 1308
Fragments arranged by size:
4631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 636 798 831 1256 1943 2190 2712 3938 4071
Size: 162 33 425 687 247 522 1226 133
Cuts at: 4071 4207 4985 5175 5967 636
Size: 136 778 190 792 1862
Fragments arranged by size:
1862 1226 792 778 687 522 425 247
190 162 136 133 33
BsmI GAATG_Cn'
Cuts at: 1004 3603 1004
Size: 2599 4594
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 2961 3065 2821
Size: 140 104 6949
Fragments arranged by size:
6949 140 104
Bsp1286I G_dGCh'C
Cuts at: 636 798 831 1121 1256 1943 2190 2255 2269
Size: 162 33 290 135 687 247 65 14
Cuts at: 2269 2712 3624 3938 4071 4207 4818 4911 4985
Size: 443 912 314 133 136 611 93 74
Cuts at: 4985 5175 5237 5967 636
Size: 190 62 730 1862
Fragments arranged by size:
1862 912 730 687 611 443 314 290
247 190 162 136 135 133 93 74
65 62 33 14
BspEI T'CCGG_A
Cuts at: 2240 2549 6413 2240
Size: 309 3864 3020
Fragments arranged by size:
3864 3020 309
BspHI T'CATG_A
Cuts at: 4154 6369 4154
Size: 2215 4978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 1805 1923 4243 4457 4759 5140 545
Size: 1260 118 2320 214 302 381 2598
Fragments arranged by size:
2598 2320 1260 381 302 214 118
BsrBI CCG'CTC
Cuts at: 50 5485 5539 7023 7188 50
Size: 5435 54 1484 165 55
Fragments arranged by size:
5435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2874 5105 2874
Size: 2231 4962
BsrFI r'CCGG_y
Cuts at: 1379 1739 5191 5372 5581 1379
Size: 360 3452 181 209 2991
Fragments arranged by size:
3452 2991 360 209 181
BssSI C'ACGA_G
Cuts at: 1942 5464 5822 1942
Size: 3522 358 3313
Fragments arranged by size:
3522 3313 358
BstAPI GCAn_nnn'nTGC
Cuts at: 793 1503 4570 793
Size: 710 3067 3416
Fragments arranged by size:
3416 3067 710
BstBI TT'CG_AA
Cuts at: 5554 5554
Size: 7193
BstXI CCAn_nnnn'nTGG
Cuts at: 783 3963 783
Size: 3180 4013
BstYI r'GATC_y
Cuts at: 1 147 176 1484 1880 2028 2206 2365 2514
Size: 146 29 1308 396 148 178 159 149
Cuts at: 2514 3666 4016 4218 4685 4708 5043 5289 6301
Size: 1152 350 202 467 23 335 246 1012
Cuts at: 6301 6387 6399 6745 1
Size: 86 12 346 449
Fragments arranged by size:
1308 1152 1012 467 449 396 350 346
335 246 202 178 159 149 148 146
86 29 23 12
BstZ17I GTA'TAC
Cuts at: 3051 3541 3051
Size: 490 6703
BtgI C'CryG_G
Cuts at: 107 107
Size: 7193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5143 5324 5487 5143
Size: 181 163 6849
Fragments arranged by size:
6849 181 163
BtsI GCAGTG_nn'
Cuts at: 2292 4436 2292
Size: 2144 5049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2046 2081 2046
Size: 35 7158
DraI TTT'AAA
Cuts at: 4192 4192
Size: 7193
DraIII CAC_nnn'GTG
Cuts at: 2004 2004
Size: 7193
DrdI GACnn_nn'nnGTC
Cuts at: 625 4899 5757 625
Size: 4274 858 2061
Fragments arranged by size:
4274 2061 858
EaeI y'GGCC_r
Cuts at: 1184 1271 2853 2880 3912 4778 4952 5343 5370
Size: 87 1582 27 1032 866 174 391 27
Cuts at: 5370 6715 1184
Size: 1345 1662
Fragments arranged by size:
1662 1582 1345 1032 866 391 174 87
27 27
EagI C'GGCC_G
Cuts at: 4778 4778
Size: 7193
EarI CTCTTCn'nnn_
Cuts at: 287 838 1088 2624 3074 5216 5426 287
Size: 551 250 1536 450 2142 210 2054
Fragments arranged by size:
2142 2054 1536 551 450 250 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5401 5711 5857 7003 5401
Size: 310 146 1146 5591
Fragments arranged by size:
5591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 829 2710 4069 4205 829
Size: 1881 1359 136 3817
Fragments arranged by size:
3817 1881 1359 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1246 1342 1600 1803 2283 2289 2586 2696
Size: 360 96 258 203 480 6 297 110
Cuts at: 2696 2782 2855 2906 3655 4151 5018 5450 6197
Size: 86 73 51 749 496 867 432 747
Cuts at: 6197 6465 886
Size: 268 1614
Fragments arranged by size:
1614 867 749 747 496 480 432 360
297 268 258 203 110 96 86 73
51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2274 2274
Size: 7193
EcoO109I rG'GnC_Cy
Cuts at: 165 1206 1912 1959 2194 4332 165
Size: 1041 706 47 235 2138 3026
Fragments arranged by size:
3026 2138 1041 706 235 47
EcoRI G'AATT_C
Cuts at: 2166 4197 2166
Size: 2031 5162
EcoRV GAT'ATC
Cuts at: 1784 3789 1784
Size: 2005 5188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 768 1537 2153 5212 768
Size: 769 616 3059 2749
Fragments arranged by size:
3059 2749 769 616
FspI TGC'GCA
Cuts at: 4974 4974
Size: 7193
HaeII r_GCGC'y
Cuts at: 859 1380 4627 4875 5897 6876 859
Size: 521 3247 248 1022 979 1176
Fragments arranged by size:
3247 1176 1022 979 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 742 774 948 980 1872 1904 2020 2052 2068
Size: 32 174 32 892 32 116 32 16
Cuts at: 2068 2100 2581 2613 3076 3079 3108 3111 3386
Size: 32 481 32 463 3 29 3 275
Cuts at: 3386 3418 4006 4038 5035 5065 5067 5097 742
Size: 32 588 32 997 30 2 30 2838
Fragments arranged by size:
2838 997 892 588 481 463 275 174
116 32 32 32 32 32 32 32
32 30 30 29 16 3 3 2
HincII GTy'rAC
Cuts at: 1151 2482 4232 1151
Size: 1331 1750 4112
Fragments arranged by size:
4112 1750 1331
KasI G'GCGC_C
Cuts at: 4623 4871 4623
Size: 248 6945
KpnI G_GTAC'C
Cuts at: 4213 4213
Size: 7193
MfeI C'AATT_G
Cuts at: 952 1543 952
Size: 591 6602
MluI A'CGCG_T
Cuts at: 6626 6626
Size: 7193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 180 1790 5864 6048 180
Size: 1610 4074 184 1325
Fragments arranged by size:
4074 1610 1325 184
MscI TGG'CCA
Cuts at: 1186 2855 2882 4954 1186
Size: 1669 27 2072 3425
Fragments arranged by size:
3425 2072 1669 27
MslI CAynn'nnrTG
Cuts at: 781 3961 4043 5182 5309 781
Size: 3180 82 1139 127 2665
Fragments arranged by size:
3180 2665 1139 127 82
MspA1I CmG'CkG
Cuts at: 1182 1224 1239 1266 1626 2789 2926 4304 4618
Size: 42 15 27 360 1163 137 1378 314
Cuts at: 4618 4978 5991 6236 1182
Size: 360 1013 245 2139
Fragments arranged by size:
2139 1378 1163 1013 360 360 314 245
137 42 27 15
NaeI GCC'GGC
Cuts at: 1381 5374 1381
Size: 3993 3200
NarI GG'CG_CC
Cuts at: 4624 4872 4624
Size: 248 6945
NcoI C'CATG_G
Cuts at: 107 107
Size: 7193
NdeI CA'TA_TG
Cuts at: 735 735
Size: 7193
NgoMIV G'CCGG_C
Cuts at: 1379 5372 1379
Size: 3993 3200
NheI G'CTAG_C
Cuts at: 2488 2488
Size: 7193
NsiI A_TGCA'T
Cuts at: 4061 4061
Size: 7193
NspI r_CATG'y
Cuts at: 324 816 4246 4746 5653 324
Size: 492 3430 500 907 1864
Fragments arranged by size:
3430 1864 907 500 492
PasI CC'CwG_GG
Cuts at: 1908 1908
Size: 7193
PciI A'CATG_T
Cuts at: 320 5649 320
Size: 5329 1864
PflMI CCAn_nnn'nTGG
Cuts at: 2004 2976 2004
Size: 972 6221
PmeI GTTT'AAAC
Cuts at: 4192 4192
Size: 7193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 2010 6332 6364 1978
Size: 32 4322 32 2807
Fragments arranged by size:
4322 2807 32 32
PpuMI rG'GwC_Cy
Cuts at: 165 1912 2194 165
Size: 1747 282 5164
Fragments arranged by size:
5164 1747 282
PsiI TTA'TAA
Cuts at: 227 3548 227
Size: 3321 3872
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1502 1534 1502
Size: 32 7161
PstI C_TGCA'G
Cuts at: 174 511 1181 1577 4240 4925 174
Size: 337 670 396 2663 685 2442
Fragments arranged by size:
2663 2442 685 670 396 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7193
PvuII CAG'CTG
Cuts at: 1182 1224 1266 2926 4618 4978 1182
Size: 42 42 1660 1692 360 3397
Fragments arranged by size:
3397 1692 1660 360 42 42
RsrII CG'GwC_CG
Cuts at: 5388 5388
Size: 7193
SacI G_AGCT'C
Cuts at: 831 2712 4071 4207 831
Size: 1881 1359 136 3817
Fragments arranged by size:
3817 1881 1359 136
SalI G'TCGA_C
Cuts at: 1149 2480 4230 1149
Size: 1331 1750 4112
Fragments arranged by size:
4112 1750 1331
SanDI GG'GwC_CC
Cuts at: 165 2194 165
Size: 2029 5164
SapI GCTCTTCn'nnn_
Cuts at: 5216 5426 5216
Size: 210 6983
SbfI CC_TGCA'GG
Cuts at: 174 4240 174
Size: 4066 3127
ScaI AGT'ACT
Cuts at: 6710 6710
Size: 7193
SexAI A'CCwGG_T
Cuts at: 2900 2900
Size: 7193
SfcI C'TryA_G
Cuts at: 33 170 507 1177 1573 2504 4236 4921 5914
Size: 137 337 670 396 931 1732 685 993
Cuts at: 5914 6105 33
Size: 191 1121
Fragments arranged by size:
1732 1121 993 931 685 670 396 337
191 137
SfoI GGC'GCC
Cuts at: 4625 4873 4625
Size: 248 6945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
SmaI CCC'GGG
Cuts at: 4215 4215
Size: 7193
SmlI C'TyrA_G
Cuts at: 1012 4348 5263 5755 6017 1012
Size: 3336 915 492 262 2188
Fragments arranged by size:
3336 2188 915 492 262
SpeI A'CTAG_T
Cuts at: 67 612 67
Size: 545 6648
SphI G_CATG'C
Cuts at: 4246 4746 4246
Size: 500 6693
SspI AAT'ATT
Cuts at: 334 351 334
Size: 17 7176
StuI AGG'CCT
Cuts at: 1641 1641
Size: 7193
StyI C'CwwG_G
Cuts at: 107 1709 1804 2334 2438 4327 4681 107
Size: 1602 95 530 104 1889 354 2619
Fragments arranged by size:
2619 1889 1602 530 354 104 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2093 3338 4776 4909 5368 5577 2093
Size: 1245 1438 133 459 209 3709
Fragments arranged by size:
3709 1438 1245 459 209 133
TatI w'GTAC_w
Cuts at: 1073 2729 6708 1073
Size: 1656 3979 1558
Fragments arranged by size:
3979 1656 1558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2169 2201 2271 2303 2402 2434 6710 6742 2169
Size: 32 70 32 99 32 4276 32 2620
Fragments arranged by size:
4276 2620 99 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4990 4990
Size: 7193
XbaI T'CTAG_A
Cuts at: 4224 4224
Size: 7193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 2923 3987 4688 270
Size: 2653 1064 701 2775
Fragments arranged by size:
2775 2653 1064 701
XmaI C'CCGG_G
Cuts at: 4213 4213
Size: 7193
XmnI GAAnn'nnTTC
Cuts at: 667 2108 2136 2166 3165 3241 667
Size: 1441 28 30 999 76 4619
Fragments arranged by size:
4619 1441 999 76 30 28
ZraI GAC'GTC
Cuts at: 2831 3059 2831
Size: 228 6965
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII ApaI AscI AvrII BaeI BbvCI BcgI
BglI Bpu10I BsiWI BsrGI BssHII BstEII Bsu36I ClaI
FseI FspAI HindIII HpaI NotI NruI PacI PfoI
PmlI PshAI PspOMI PspXI SacII SfiI SgrAI SnaBI
SrfI SwaI XhoI