(Circular) (Six-base) MAPSORT of: pF1KA0553.seq Check: 8107 from: 1 to: 7385 pF1KA0553 7385 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1779 1779 Size: 7385 AccI GT'mk_AC Cuts at: 2526 4423 2526 Size: 1897 5488 Acc65I G'GTAC_C Cuts at: 4401 4401 Size: 7385 AclI AA'CG_TT Cuts at: 7225 7225 Size: 7385 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 167 400 878 1760 2177 2489 3140 3440 5210 Size: 233 478 882 417 312 651 300 1770 Cuts at: 5210 5642 6389 6657 167 Size: 432 747 268 895 Fragments arranged by size: 1770 895 882 747 651 478 432 417 312 300 268 233 AfeI AGC'GCT Cuts at: 2593 2731 2593 Size: 138 7247 AflIII A'CryG_T Cuts at: 4737 5841 6818 4737 Size: 1104 977 5304 Fragments arranged by size: 5304 1104 977 AgeI A'CCGG_T Cuts at: 5773 5773 Size: 7385 AleI CACnn'nnGTG Cuts at: 2234 2234 Size: 7385 AlwNI CAG_nnn'CTG Cuts at: 538 1045 1783 2053 3664 3879 3976 4036 4297 Size: 507 738 270 1611 215 97 60 261 Cuts at: 4297 4353 4690 6257 538 Size: 56 337 1567 1666 Fragments arranged by size: 1666 1611 1567 738 507 337 270 261 215 97 60 56 ApaI G_GGCC'C Cuts at: 3236 3272 3416 4226 3236 Size: 36 144 810 6395 Fragments arranged by size: 6395 810 144 36 ApaLI G'TGCA_C Cuts at: 497 6155 497 Size: 5658 1727 ApoI r'AATT_y Cuts at: 322 628 4389 6738 6994 322 Size: 306 3761 2349 256 713 Fragments arranged by size: 3761 2349 713 306 256 AseI AT'TA_AT Cuts at: 20 1540 20 Size: 1520 5865 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7385 AvaI C'yCGr_G Cuts at: 2741 4405 2741 Size: 1664 5721 BamHI G'GATC_C Cuts at: 3735 4410 3735 Size: 675 6710 BanI G'GyrC_C Cuts at: 164 598 2272 2447 3547 4401 4815 5063 5098 Size: 434 1674 175 1100 854 414 248 35 Cuts at: 5098 164 Size: 2451 Fragments arranged by size: 2451 1674 1100 854 434 414 248 175 35 BanII G_rGCy'C Cuts at: 1292 2124 2869 2917 3236 3272 3416 4226 4399 Size: 832 745 48 319 36 144 810 173 Cuts at: 4399 5429 1292 Size: 1030 3248 Fragments arranged by size: 3248 1030 832 810 745 319 173 144 48 36 BbeI G_GCGC'C Cuts at: 168 2451 4819 5067 168 Size: 2283 2368 248 2486 Fragments arranged by size: 2486 2368 2283 248 BbsI GAAGACnn'nnnn_ Cuts at: 197 269 965 1847 3128 197 Size: 72 696 882 1281 4454 Fragments arranged by size: 4454 1281 882 696 72 BbvCI CC'TCA_GC Cuts at: 1313 3764 3909 4206 1313 Size: 2451 145 297 4492 Fragments arranged by size: 4492 2451 297 145 BciVI GTATCCnnnnn_n' Cuts at: 5283 6044 7385 5283 Size: 761 1341 5283 Fragments arranged by size: 5283 1341 761 BclI T'GATC_A Cuts at: 348 1347 3525 4905 348 Size: 999 2178 1380 2828 Fragments arranged by size: 2828 2178 1380 999 BglI GCCn_nnn'nGGC Cuts at: 793 793 Size: 7385 BglII A'GATC_T Cuts at: 375 2960 4900 6937 375 Size: 2585 1940 2037 823 Fragments arranged by size: 2585 2037 1940 823 BlpI GC'TnA_GC Cuts at: 3854 4497 3854 Size: 643 6742 Bme1580I G_kGCm'C Cuts at: 501 3236 3272 3416 4226 5010 5103 6159 501 Size: 2735 36 144 810 784 93 1056 1727 Fragments arranged by size: 2735 1727 1056 810 784 144 93 36 BmrI ACTGGGnnnn_n' Cuts at: 1483 2306 2370 2967 3608 3707 3722 4597 4678 Size: 823 64 597 641 99 15 875 81 Cuts at: 4678 5013 7157 1483 Size: 335 2144 1711 Fragments arranged by size: 2144 1711 875 823 641 597 335 99 81 64 15 BmtI G_CTAG'C Cuts at: 4181 4181 Size: 7385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1422 1986 5752 1422 Size: 564 3766 3055 Fragments arranged by size: 3766 3055 564 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1504 2871 3175 3530 3652 3826 1504 Size: 1367 304 355 122 174 5063 Fragments arranged by size: 5063 1367 355 304 174 122 Bpu10I CC'TnA_GC Cuts at: 1313 3764 3909 4206 1313 Size: 2451 145 297 4492 Fragments arranged by size: 4492 2451 297 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 2418 2469 2718 3226 3721 4561 5440 5932 Size: 310 51 249 508 495 840 879 492 Cuts at: 5932 6230 2108 Size: 298 3263 Fragments arranged by size: 3263 879 840 508 495 492 310 298 249 51 BsaI GGTCTCn'nnnn_ Cuts at: 1378 7089 1378 Size: 5711 1674 BsaAI yAC'GTr Cuts at: 4738 4738 Size: 7385 BsaBI GATnn'nnATC Cuts at: 6 4899 6936 6 Size: 4893 2037 455 Fragments arranged by size: 4893 2037 455 BsaHI Gr'CG_yC Cuts at: 165 2448 2937 4816 5064 5766 7262 165 Size: 2283 489 1879 248 702 1496 288 Fragments arranged by size: 2283 1879 1496 702 489 288 248 BsaWI w'CCGG_w Cuts at: 4574 5095 5773 6047 6194 6605 4574 Size: 521 678 274 147 411 5354 Fragments arranged by size: 5354 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1325 1355 2076 2106 2781 2811 1325 Size: 30 721 30 675 30 5899 Fragments arranged by size: 5899 721 675 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1656 2935 3050 3217 3832 1656 Size: 1279 115 167 615 5209 Fragments arranged by size: 5209 1279 615 167 115 BseYI C'CCAG_C Cuts at: 1030 1068 1434 1861 2043 2135 2151 3229 3327 Size: 38 366 427 182 92 16 1078 98 Cuts at: 3327 3552 3924 4100 4366 4810 5568 6145 6641 Size: 225 372 176 266 444 758 577 496 Cuts at: 6641 1030 Size: 1774 Fragments arranged by size: 1774 1078 758 577 496 444 427 372 366 266 225 182 176 98 92 38 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2341 3229 4289 2341 Size: 888 1060 5437 Fragments arranged by size: 5437 1060 888 BsiEI CG_ry'CG Cuts at: 104 4927 4973 6181 104 Size: 4823 46 1208 1308 Fragments arranged by size: 4823 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 501 2124 4399 5177 5367 6159 501 Size: 1623 2275 778 190 792 1727 Fragments arranged by size: 2275 1727 1623 792 778 190 BsmI GAATG_Cn' Cuts at: 1167 2418 2928 4049 1167 Size: 1251 510 1121 4503 Fragments arranged by size: 4503 1251 1121 510 Bsp1286I G_dGCh'C Cuts at: 501 1292 2124 2869 2917 3236 3272 3416 4226 Size: 791 832 745 48 319 36 144 810 Cuts at: 4226 4399 5010 5103 5177 5367 5429 6159 501 Size: 173 611 93 74 190 62 730 1727 Fragments arranged by size: 1727 832 810 791 745 730 611 319 190 173 144 93 74 62 48 36 BspEI T'CCGG_A Cuts at: 6605 6605 Size: 7385 BspHI T'CATG_A Cuts at: 6561 6561 Size: 7385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1779 4361 4435 4649 4951 5332 1779 Size: 2582 74 214 302 381 3832 Fragments arranged by size: 3832 2582 381 302 214 74 BsrBI CCG'CTC Cuts at: 50 2432 2969 5677 5731 7215 7380 50 Size: 2382 537 2708 54 1484 165 55 Fragments arranged by size: 2708 2382 1484 537 165 55 54 BsrDI GCAATG_nn' Cuts at: 754 5297 754 Size: 4543 2842 BsrFI r'CCGG_y Cuts at: 1892 2288 2351 2444 5383 5564 5773 1892 Size: 396 63 93 2939 181 209 3504 Fragments arranged by size: 3504 2939 396 209 181 93 63 BssSI C'ACGA_G Cuts at: 2908 5656 6014 2908 Size: 2748 358 4279 Fragments arranged by size: 4279 2748 358 BstAPI GCAn_nnn'nTGC Cuts at: 4036 4297 4762 4036 Size: 261 465 6659 Fragments arranged by size: 6659 465 261 BstBI TT'CG_AA Cuts at: 5746 5746 Size: 7385 BstXI CCAn_nnnn'nTGG Cuts at: 114 258 687 700 2215 114 Size: 144 429 13 1515 5284 Fragments arranged by size: 5284 1515 429 144 13 BstYI r'GATC_y Cuts at: 1 375 415 622 1692 2143 2737 2960 3161 Size: 374 40 207 1070 451 594 223 201 Cuts at: 3161 3735 4410 4877 4900 5235 5481 6493 6579 Size: 574 675 467 23 335 246 1012 86 Cuts at: 6579 6591 6937 1 Size: 12 346 449 Fragments arranged by size: 1070 1012 675 594 574 467 451 449 374 346 335 246 223 207 201 86 40 23 12 Bsu36I CC'TnA_GG Cuts at: 965 1329 1749 2918 3815 965 Size: 364 420 1169 897 4535 Fragments arranged by size: 4535 1169 897 420 364 BtgI C'CryG_G Cuts at: 107 1824 2214 4279 4371 107 Size: 1717 390 2065 92 3121 Fragments arranged by size: 3121 2065 1717 390 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5335 5516 5679 5335 Size: 181 163 7041 Fragments arranged by size: 7041 181 163 BtsI GCAGTG_nn' Cuts at: 602 656 1074 1511 2175 2418 3137 4301 4628 Size: 54 418 437 664 243 719 1164 327 Cuts at: 4628 602 Size: 3359 Fragments arranged by size: 3359 1164 719 664 437 418 327 243 54 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 515 550 3158 3193 4254 4289 515 Size: 35 2608 35 1061 35 3611 Fragments arranged by size: 3611 2608 1061 35 35 35 DraI TTT'AAA Cuts at: 1602 4384 1602 Size: 2782 4603 DraIII CAC_nnn'GTG Cuts at: 3534 3534 Size: 7385 DrdI GACnn_nn'nnGTC Cuts at: 5091 5949 5091 Size: 858 6527 EaeI y'GGCC_r Cuts at: 1427 1525 2442 3881 4155 4970 5144 5535 5562 Size: 98 917 1439 274 815 174 391 27 Cuts at: 5562 6907 1427 Size: 1345 1905 Fragments arranged by size: 1905 1439 1345 917 815 391 274 174 98 27 EagI C'GGCC_G Cuts at: 4970 4970 Size: 7385 EarI CTCTTCn'nnn_ Cuts at: 194 414 877 1413 1955 2153 2259 2390 2508 Size: 220 463 536 542 198 106 131 118 Cuts at: 2508 2887 3087 3353 3377 3401 3491 5408 5618 Size: 379 200 266 24 24 90 1917 210 Cuts at: 5618 194 Size: 1961 Fragments arranged by size: 1961 1917 542 536 463 379 266 220 210 200 198 131 118 106 90 24 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1742 2130 5593 5903 6049 7195 1742 Size: 388 3463 310 146 1146 1932 Fragments arranged by size: 3463 1932 1146 388 310 146 EcoICRI GAG'CTC Cuts at: 2122 4397 2122 Size: 2275 5110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 167 400 878 1504 1760 2177 2489 2871 3140 Size: 233 478 626 256 417 312 382 269 Cuts at: 3140 3175 3440 3530 3652 3826 5210 5642 6389 Size: 35 265 90 122 174 1384 432 747 Cuts at: 6389 6657 167 Size: 268 895 Fragments arranged by size: 1384 895 747 626 478 432 417 382 312 269 268 265 256 233 174 122 90 35 EcoNI CCTnn'n_nnAGG Cuts at: 1775 1775 Size: 7385 EcoO109I rG'GnC_Cy Cuts at: 649 749 851 977 1459 2062 3010 3246 3412 Size: 100 102 126 482 603 948 236 166 Cuts at: 3412 3413 3579 3661 4223 4350 4524 649 Size: 1 166 82 562 127 174 3510 Fragments arranged by size: 3510 948 603 562 482 236 174 166 166 127 126 102 100 82 1 EcoRI G'AATT_C Cuts at: 628 4389 628 Size: 3761 3624 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1344 2257 5404 1344 Size: 913 3147 3325 Fragments arranged by size: 3325 3147 913 FspI TGC'GCA Cuts at: 5166 5166 Size: 7385 HaeII r_GCGC'y Cuts at: 168 2451 2595 2733 4819 5067 6089 7068 168 Size: 2283 144 138 2086 248 1022 979 485 Fragments arranged by size: 2283 2086 1022 979 485 248 144 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 918 950 961 993 2076 2108 2329 2361 3115 Size: 32 11 32 1083 32 221 32 754 Cuts at: 3115 3147 3490 3522 5227 5257 5259 5289 918 Size: 32 343 32 1705 30 2 30 3014 Fragments arranged by size: 3014 1705 1083 754 343 221 32 32 32 32 32 32 30 30 11 2 HincII GTy'rAC Cuts at: 231 3752 4424 231 Size: 3521 672 3192 Fragments arranged by size: 3521 3192 672 KasI G'GCGC_C Cuts at: 164 2447 4815 5063 164 Size: 2283 2368 248 2486 Fragments arranged by size: 2486 2368 2283 248 KpnI G_GTAC'C Cuts at: 4405 4405 Size: 7385 MfeI C'AATT_G Cuts at: 245 245 Size: 7385 MluI A'CGCG_T Cuts at: 6818 6818 Size: 7385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1427 6056 6240 1427 Size: 4629 184 2572 Fragments arranged by size: 4629 2572 184 MscI TGG'CCA Cuts at: 1429 1527 3883 4157 5146 1429 Size: 98 2356 274 989 3668 Fragments arranged by size: 3668 2356 989 274 98 MslI CAynn'nnrTG Cuts at: 112 127 2234 5374 5501 112 Size: 15 2107 3140 127 1996 Fragments arranged by size: 3140 2107 1996 127 15 MspA1I CmG'CkG Cuts at: 2139 2155 2816 3913 4033 4297 4496 4810 5170 Size: 16 661 1097 120 264 199 314 360 Cuts at: 5170 6183 6428 2139 Size: 1013 245 3096 Fragments arranged by size: 3096 1097 1013 661 360 314 264 245 199 120 16 NaeI GCC'GGC Cuts at: 2446 5566 2446 Size: 3120 4265 NarI GG'CG_CC Cuts at: 165 2448 4816 5064 165 Size: 2283 2368 248 2486 Fragments arranged by size: 2486 2368 2283 248 NcoI C'CATG_G Cuts at: 107 1824 2214 4371 107 Size: 1717 390 2157 3121 Fragments arranged by size: 3121 2157 1717 390 NgoMIV G'CCGG_C Cuts at: 2444 5564 2444 Size: 3120 4265 NheI G'CTAG_C Cuts at: 4177 4177 Size: 7385 NspI r_CATG'y Cuts at: 4006 4252 4438 4938 5845 4006 Size: 246 186 500 907 5546 Fragments arranged by size: 5546 907 500 246 186 PasI CC'CwG_GG Cuts at: 2080 3418 3574 2080 Size: 1338 156 5891 Fragments arranged by size: 5891 1338 156 PciI A'CATG_T Cuts at: 5841 5841 Size: 7385 PflMI CCAn_nnn'nTGG Cuts at: 468 3289 468 Size: 2821 4564 PfoI T'CCnGG_A Cuts at: 2887 2887 Size: 7385 PmeI GTTT'AAAC Cuts at: 4384 4384 Size: 7385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6524 6556 6524 Size: 32 7353 PpuMI rG'GwC_Cy Cuts at: 749 851 977 1459 2062 3010 3246 3579 4350 Size: 102 126 482 603 948 236 333 771 Cuts at: 4350 749 Size: 3784 Fragments arranged by size: 3784 948 771 603 482 333 236 126 102 PshAI GACnn'nnGTC Cuts at: 3113 3113 Size: 7385 PspOMI G'GGCC_C Cuts at: 3232 3268 3412 4222 3232 Size: 36 144 810 6395 Fragments arranged by size: 6395 810 144 36 PstI C_TGCA'G Cuts at: 663 905 3115 4293 4432 5117 663 Size: 242 2210 1178 139 685 2931 Fragments arranged by size: 2931 2210 1178 685 242 139 PvuI CG_AT'CG Cuts at: 104 104 Size: 7385 PvuII CAG'CTG Cuts at: 2155 2816 3913 4033 4297 4810 5170 2155 Size: 661 1097 120 264 513 360 4370 Fragments arranged by size: 4370 1097 661 513 360 264 120 RsrII CG'GwC_CG Cuts at: 5580 5580 Size: 7385 SacI G_AGCT'C Cuts at: 2124 4399 2124 Size: 2275 5110 SalI G'TCGA_C Cuts at: 4422 4422 Size: 7385 SanDI GG'GwC_CC Cuts at: 749 977 3579 749 Size: 228 2602 4555 Fragments arranged by size: 4555 2602 228 SapI GCTCTTCn'nnn_ Cuts at: 194 2259 3377 5408 5618 194 Size: 2065 1118 2031 210 1961 Fragments arranged by size: 2065 2031 1961 1118 210 SbfI CC_TGCA'GG Cuts at: 4432 4432 Size: 7385 ScaI AGT'ACT Cuts at: 6902 6902 Size: 7385 SfcI C'TryA_G Cuts at: 33 539 659 901 1037 1788 2520 2553 2562 Size: 506 120 242 136 751 732 33 9 Cuts at: 2562 2676 2835 3111 3686 3968 4289 4428 5113 Size: 114 159 276 575 282 321 139 685 Cuts at: 5113 6106 6297 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 751 732 685 575 506 321 282 276 242 191 159 139 136 120 114 33 9 SfoI GGC'GCC Cuts at: 166 2449 4817 5065 166 Size: 2283 2368 248 2486 Fragments arranged by size: 2486 2368 2283 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7385 SmaI CCC'GGG Cuts at: 2743 4407 2743 Size: 1664 5721 SmlI C'TyrA_G Cuts at: 2123 2433 2484 2733 3241 3700 4540 5455 5947 Size: 310 51 249 508 459 840 915 492 Cuts at: 5947 6209 2123 Size: 262 3299 Fragments arranged by size: 3299 915 840 508 492 459 310 262 249 51 SpeI A'CTAG_T Cuts at: 67 67 Size: 7385 SphI G_CATG'C Cuts at: 4006 4438 4938 4006 Size: 432 500 6453 Fragments arranged by size: 6453 500 432 SspI AAT'ATT Cuts at: 2656 2656 Size: 7385 StuI AGG'CCT Cuts at: 1327 1688 3898 1327 Size: 361 2210 4814 Fragments arranged by size: 4814 2210 361 StyI C'CwwG_G Cuts at: 107 257 714 894 1227 1550 1683 1824 2214 Size: 150 457 180 333 323 133 141 390 Cuts at: 2214 4371 4519 4873 107 Size: 2157 148 354 2619 Fragments arranged by size: 2619 2157 457 390 354 333 323 180 150 148 141 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1169 1300 4334 4968 5101 5560 5769 1169 Size: 131 3034 634 133 459 209 2785 Fragments arranged by size: 3034 2785 634 459 209 133 131 TatI w'GTAC_w Cuts at: 215 663 2378 3830 6900 215 Size: 448 1715 1452 3070 700 Fragments arranged by size: 3070 1715 1452 700 448 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1906 1938 3177 3209 6902 6934 1906 Size: 32 1239 32 3693 32 2357 Fragments arranged by size: 3693 2357 1239 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5182 5182 Size: 7385 XbaI T'CTAG_A Cuts at: 4416 4416 Size: 7385 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1128 2215 2314 3150 4880 1128 Size: 1087 99 836 1730 3633 Fragments arranged by size: 3633 1730 1087 836 99 XmaI C'CCGG_G Cuts at: 2741 4405 2741 Size: 1664 5721 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AlfI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI NdeI NotI NruI NsiI PacI PmlI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI