Restriction map for
pF1KA0555
(Circular) (Six-base) MAPSORT of: pF1KA0555.seq Check: 4133 from: 1 to: 5510
pF1KA0555 5510 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 591 904 591
Size: 313 5197
AccI GT'mk_AC
Cuts at: 2548 2548
Size: 5510
Acc65I G'GTAC_C
Cuts at: 2526 2526
Size: 5510
AclI AA'CG_TT
Cuts at: 5350 5350
Size: 5510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1240 1273 1541 2338 3335 3767 4514 4782
Size: 432 33 268 797 997 432 747 268
Cuts at: 4782 808
Size: 1536
Fragments arranged by size:
1536 997 797 747 432 432 268 268
33
AfeI AGC'GCT
Cuts at: 503 503
Size: 5510
AflII C'TTAA_G
Cuts at: 556 556
Size: 5510
AflIII A'CryG_T
Cuts at: 1050 2862 3966 4943 1050
Size: 1812 1104 977 1617
Fragments arranged by size:
1812 1617 1104 977
AgeI A'CCGG_T
Cuts at: 1748 3898 1748
Size: 2150 3360
AlwNI CAG_nnn'CTG
Cuts at: 2815 4382 2815
Size: 1567 3943
ApaLI G'TGCA_C
Cuts at: 4280 4280
Size: 5510
ApoI r'AATT_y
Cuts at: 899 1179 1856 1974 2393 2514 4863 5119 899
Size: 280 677 118 419 121 2349 256 1290
Fragments arranged by size:
2349 1290 677 419 280 256 121 118
AseI AT'TA_AT
Cuts at: 20 20
Size: 5510
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5510
AvaI C'yCGr_G
Cuts at: 142 518 2530 142
Size: 376 2012 3122
Fragments arranged by size:
3122 2012 376
BamHI G'GATC_C
Cuts at: 2042 2535 2042
Size: 493 5017
BanI G'GyrC_C
Cuts at: 2526 2940 3188 3223 2526
Size: 414 248 35 4813
Fragments arranged by size:
4813 414 248 35
BanII G_rGCy'C
Cuts at: 1325 2372 2524 3554 1325
Size: 1047 152 1030 3281
Fragments arranged by size:
3281 1047 1030 152
BbeI G_GCGC'C
Cuts at: 2944 3192 2944
Size: 248 5262
BbsI GAAGACnn'nnnn_
Cuts at: 185 265 1010 1694 185
Size: 80 745 684 4001
Fragments arranged by size:
4001 745 684 80
BbvCI CC'TCA_GC
Cuts at: 2063 2284 2320 2063
Size: 221 36 5253
Fragments arranged by size:
5253 221 36
BciVI GTATCCnnnnn_n'
Cuts at: 3408 4169 5510 3408
Size: 761 1341 3408
Fragments arranged by size:
3408 1341 761
BclI T'GATC_A
Cuts at: 3030 3030
Size: 5510
BglII A'GATC_T
Cuts at: 1892 3025 5062 1892
Size: 1133 2037 2340
Fragments arranged by size:
2340 2037 1133
BlpI GC'TnA_GC
Cuts at: 2622 2622
Size: 5510
Bme1580I G_kGCm'C
Cuts at: 3135 3228 4284 3135
Size: 93 1056 4361
Fragments arranged by size:
4361 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2722 2803 3138 5282 2722
Size: 81 335 2144 2950
Fragments arranged by size:
2950 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1360 3877 1360
Size: 2517 2993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 808 1816 2158 310
Size: 498 1008 342 3662
Fragments arranged by size:
3662 1008 498 342
Bpu10I CC'TnA_GC
Cuts at: 1666 2063 2284 2320 1666
Size: 397 221 36 4856
Fragments arranged by size:
4856 397 221 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 433 1625 2686 3565 4057 4355 248
Size: 185 1192 1061 879 492 298 1403
Fragments arranged by size:
1403 1192 1061 879 492 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 784 1772 5214 784
Size: 988 3442 1080
Fragments arranged by size:
3442 1080 988
BsaAI yAC'GTr
Cuts at: 427 2863 427
Size: 2436 3074
BsaBI GATnn'nnATC
Cuts at: 6 3024 5061 6
Size: 3018 2037 455
Fragments arranged by size:
3018 2037 455
BsaHI Gr'CG_yC
Cuts at: 1772 2941 3189 3891 5387 1772
Size: 1169 248 702 1496 1895
Fragments arranged by size:
1895 1496 1169 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2699 3220 3898 4172 4319 4730 1748
Size: 951 521 678 274 147 411 2528
Fragments arranged by size:
2528 951 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 800 830 856 886 800
Size: 30 26 30 5424
Fragments arranged by size:
5424 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 538 2293 358
Size: 180 1755 3575
Fragments arranged by size:
3575 1755 180
BseYI C'CCAG_C
Cuts at: 649 2935 3693 4270 4766 649
Size: 2286 758 577 496 1393
Fragments arranged by size:
2286 1393 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3052 3098 4306 104
Size: 2948 46 1208 1308
Fragments arranged by size:
2948 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 468 1325 1887 2372 2524 3302 3492 4284 468
Size: 857 562 485 152 778 190 792 1694
Fragments arranged by size:
1694 857 792 778 562 485 190 152
BsmI GAATG_Cn'
Cuts at: 987 987
Size: 5510
BsmBI CGTCTCn'nnnn_
Cuts at: 1824 1824
Size: 5510
Bsp1286I G_dGCh'C
Cuts at: 468 1325 1887 2372 2524 3135 3228 3302 3492
Size: 857 562 485 152 611 93 74 190
Cuts at: 3492 3554 4284 468
Size: 62 730 1694
Fragments arranged by size:
1694 857 730 611 562 485 190 152
93 74 62
BspEI T'CCGG_A
Cuts at: 4730 4730
Size: 5510
BspHI T'CATG_A
Cuts at: 4686 4686
Size: 5510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 904 2560 2774 3076 3457 591
Size: 313 1656 214 302 381 2644
Fragments arranged by size:
2644 1656 381 313 302 214
BsrBI CCG'CTC
Cuts at: 50 717 3802 3856 5340 5505 50
Size: 667 3085 54 1484 165 55
Fragments arranged by size:
3085 1484 667 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1078 3422 1078
Size: 2344 3166
BsrFI r'CCGG_y
Cuts at: 1748 3508 3689 3898 1748
Size: 1760 181 209 3360
Fragments arranged by size:
3360 1760 209 181
BsrGI T'GTAC_A
Cuts at: 807 2213 807
Size: 1406 4104
BssSI C'ACGA_G
Cuts at: 394 3781 4139 394
Size: 3387 358 1765
Fragments arranged by size:
3387 1765 358
BstAPI GCAn_nnn'nTGC
Cuts at: 309 2887 309
Size: 2578 2932
BstBI TT'CG_AA
Cuts at: 1803 3871 1803
Size: 2068 3442
BstYI r'GATC_y
Cuts at: 1 437 1892 2042 2535 3002 3025 3360 3606
Size: 436 1455 150 493 467 23 335 246
Cuts at: 3606 4618 4704 4716 5062 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1455 1012 493 467 449 436 346 335
246 150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 184 184
Size: 5510
BtgI C'CryG_G
Cuts at: 1451 1451
Size: 5510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3460 3641 3804 3460
Size: 181 163 5166
Fragments arranged by size:
5166 181 163
BtsI GCAGTG_nn'
Cuts at: 489 930 2753 489
Size: 441 1823 3246
Fragments arranged by size:
3246 1823 441
DraI TTT'AAA
Cuts at: 2509 2509
Size: 5510
DraIII CAC_nnn'GTG
Cuts at: 309 309
Size: 5510
DrdI GACnn_nn'nnGTC
Cuts at: 3216 4074 3216
Size: 858 4652
EaeI y'GGCC_r
Cuts at: 590 1997 3095 3269 3660 3687 5032 590
Size: 1407 1098 174 391 27 1345 1068
Fragments arranged by size:
1407 1345 1098 1068 391 174 27
EagI C'GGCC_G
Cuts at: 3095 3095
Size: 5510
EarI CTCTTCn'nnn_
Cuts at: 268 1814 2320 3533 3743 268
Size: 1546 506 1213 210 2035
Fragments arranged by size:
2035 1546 1213 506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3718 4028 4174 5320 3718
Size: 310 146 1146 3908
Fragments arranged by size:
3908 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1323 2370 2522 1323
Size: 1047 152 4311
Fragments arranged by size:
4311 1047 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 808 1240 1273 1541 1816 2158 2338 3335
Size: 498 432 33 268 275 342 180 997
Cuts at: 3335 3767 4514 4782 310
Size: 432 747 268 1038
Fragments arranged by size:
1038 997 747 498 432 432 342 275
268 268 180 33
EcoNI CCTnn'n_nnAGG
Cuts at: 778 1591 778
Size: 813 4697
EcoO109I rG'GnC_Cy
Cuts at: 654 1566 2649 654
Size: 912 1083 3515
Fragments arranged by size:
3515 1083 912
EcoRI G'AATT_C
Cuts at: 2393 2514 2393
Size: 121 5389
EcoRV GAT'ATC
Cuts at: 1956 1956
Size: 5510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3529 3529
Size: 5510
FspI TGC'GCA
Cuts at: 3291 3291
Size: 5510
HaeII r_GCGC'y
Cuts at: 505 555 2944 3192 4214 5193 505
Size: 50 2389 248 1022 979 822
Fragments arranged by size:
2389 1022 979 822 248 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 654 658 686 690 1411 1432 1443 1464 1598
Size: 4 28 4 721 21 11 21 134
Cuts at: 1598 1630 1692 1724 1819 1851 3352 3382 3384
Size: 32 62 32 95 32 1501 30 2
Cuts at: 3384 3414 654
Size: 30 2750
Fragments arranged by size:
2750 1501 721 134 95 62 32 32
32 30 30 28 21 21 11 4
4 2
HincII GTy'rAC
Cuts at: 2549 2549
Size: 5510
HindIII A'AGCT_T
Cuts at: 244 244
Size: 5510
KasI G'GCGC_C
Cuts at: 2940 3188 2940
Size: 248 5262
KpnI G_GTAC'C
Cuts at: 2530 2530
Size: 5510
MluI A'CGCG_T
Cuts at: 4943 4943
Size: 5510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 692 843 4181 4365 692
Size: 151 3338 184 1837
Fragments arranged by size:
3338 1837 184 151
MscI TGG'CCA
Cuts at: 1999 3271 1999
Size: 1272 4238
MslI CAynn'nnrTG
Cuts at: 3499 3626 3499
Size: 127 5383
MspA1I CmG'CkG
Cuts at: 1519 2621 2935 3295 4308 4553 1519
Size: 1102 314 360 1013 245 2476
Fragments arranged by size:
2476 1102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3691 3691
Size: 5510
NarI GG'CG_CC
Cuts at: 2941 3189 2941
Size: 248 5262
NcoI C'CATG_G
Cuts at: 1451 1451
Size: 5510
NdeI CA'TA_TG
Cuts at: 1581 1581
Size: 5510
NgoMIV G'CCGG_C
Cuts at: 3689 3689
Size: 5510
NspI r_CATG'y
Cuts at: 2563 3063 3970 2563
Size: 500 907 4103
Fragments arranged by size:
4103 907 500
PciI A'CATG_T
Cuts at: 3966 3966
Size: 5510
PfoI T'CCnGG_A
Cuts at: 903 1085 2192 903
Size: 182 1107 4221
Fragments arranged by size:
4221 1107 182
PmeI GTTT'AAAC
Cuts at: 2509 2509
Size: 5510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 5478
PpuMI rG'GwC_Cy
Cuts at: 654 654
Size: 5510
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1467 1499 1467
Size: 32 5478
PstI C_TGCA'G
Cuts at: 365 937 2557 3242 365
Size: 572 1620 685 2633
Fragments arranged by size:
2633 1620 685 572
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5510
PvuII CAG'CTG
Cuts at: 1519 2935 3295 1519
Size: 1416 360 3734
Fragments arranged by size:
3734 1416 360
RsrII CG'GwC_CG
Cuts at: 3705 3705
Size: 5510
SacI G_AGCT'C
Cuts at: 1325 2372 2524 1325
Size: 1047 152 4311
Fragments arranged by size:
4311 1047 152
SalI G'TCGA_C
Cuts at: 2547 2547
Size: 5510
SapI GCTCTTCn'nnn_
Cuts at: 2320 3533 3743 2320
Size: 1213 210 4087
Fragments arranged by size:
4087 1213 210
SbfI CC_TGCA'GG
Cuts at: 2557 2557
Size: 5510
ScaI AGT'ACT
Cuts at: 5027 5027
Size: 5510
SfcI C'TryA_G
Cuts at: 33 361 933 1591 1765 1888 2154 2224 2553
Size: 328 572 658 174 123 266 70 329
Cuts at: 2553 3238 4231 4422 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 572 329 328 266
191 174 123 70
SfoI GGC'GCC
Cuts at: 2942 3190 2942
Size: 248 5262
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5510
SmaI CCC'GGG
Cuts at: 520 2532 520
Size: 2012 3498
SmlI C'TyrA_G
Cuts at: 263 448 556 1640 2665 3580 4072 4334 263
Size: 185 108 1084 1025 915 492 262 1439
Fragments arranged by size:
1439 1084 1025 915 492 262 185 108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5510
SphI G_CATG'C
Cuts at: 2563 3063 2563
Size: 500 5010
StuI AGG'CCT
Cuts at: 1392 1392
Size: 5510
StyI C'CwwG_G
Cuts at: 233 752 1172 1451 1634 2644 2998 233
Size: 519 420 279 183 1010 354 2745
Fragments arranged by size:
2745 1010 519 420 354 279 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1349 1877 1951 3093 3226 3685 3894 1349
Size: 528 74 1142 133 459 209 2965
Fragments arranged by size:
2965 1142 528 459 209 133 74
TatI w'GTAC_w
Cuts at: 807 2213 5025 807
Size: 1406 2812 1292
Fragments arranged by size:
2812 1406 1292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 5027 5059 856
Size: 32 4139 32 1307
Fragments arranged by size:
4139 1307 32 32
Tth111I GACn'n_nGTC
Cuts at: 3307 3307
Size: 5510
XbaI T'CTAG_A
Cuts at: 2541 2541
Size: 5510
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 3005 600
Size: 2405 3105
XmaI C'CCGG_G
Cuts at: 518 2530 518
Size: 2012 3498
XmnI GAAnn'nnTTC
Cuts at: 1371 2456 1371
Size: 1085 4425
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BssHII BstEII BstXI BstZ17I ClaI CspCI FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI