Restriction map for
pF1KA0556
(Circular) (Six-base) MAPSORT of: pF1KA0556.seq Check: 7607 from: 1 to: 7967
pF1KA0556 7967 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3848 3848
Size: 7967
AatII G_ACGT'C
Cuts at: 4472 4472
Size: 7967
AccI GT'mk_AC
Cuts at: 3055 5005 3055
Size: 1950 6017
Acc65I G'GTAC_C
Cuts at: 4983 4983
Size: 7967
AclI AA'CG_TT
Cuts at: 7807 7807
Size: 7967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 470 3727 5792 6224 6971 7239 217
Size: 253 3257 2065 432 747 268 945
Fragments arranged by size:
3257 2065 945 747 432 268 253
AfeI AGC'GCT
Cuts at: 3034 3034
Size: 7967
AflII C'TTAA_G
Cuts at: 459 459
Size: 7967
AflIII A'CryG_T
Cuts at: 4526 4735 5319 6423 7400 4526
Size: 209 584 1104 977 5093
Fragments arranged by size:
5093 1104 977 584 209
AgeI A'CCGG_T
Cuts at: 3120 6355 3120
Size: 3235 4732
AhdI GACnn_n'nnGTC
Cuts at: 2181 2739 3051 3076 2181
Size: 558 312 25 7072
Fragments arranged by size:
7072 558 312 25
AleI CACnn'nnGTG
Cuts at: 883 883
Size: 7967
AlwNI CAG_nnn'CTG
Cuts at: 123 541 651 2992 3828 4221 5272 6839 123
Size: 418 110 2341 836 393 1051 1567 1251
Fragments arranged by size:
2341 1567 1251 1051 836 418 393 110
ApaLI G'TGCA_C
Cuts at: 6737 6737
Size: 7967
ApoI r'AATT_y
Cuts at: 895 933 2154 3329 3750 4971 7320 7576 895
Size: 38 1221 1175 421 1221 2349 256 1286
Fragments arranged by size:
2349 1286 1221 1221 1175 421 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 7967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7967
AvaI C'yCGr_G
Cuts at: 2350 2440 2865 3505 3883 4063 4987 2350
Size: 90 425 640 378 180 924 5330
Fragments arranged by size:
5330 924 640 425 378 180 90
BamHI G'GATC_C
Cuts at: 3659 4691 4992 3659
Size: 1032 301 6634
Fragments arranged by size:
6634 1032 301
BanI G'GyrC_C
Cuts at: 331 509 1864 2032 2050 3928 4284 4983 5397
Size: 178 1355 168 18 1878 356 699 414
Cuts at: 5397 5645 5680 331
Size: 248 35 2618
Fragments arranged by size:
2618 1878 1355 699 414 356 248 178
168 35 18
BanII G_rGCy'C
Cuts at: 2297 2677 2812 2864 2888 3456 3677 3791 3881
Size: 380 135 52 24 568 221 114 90
Cuts at: 3881 4215 4745 4981 6011 2297
Size: 334 530 236 1030 4253
Fragments arranged by size:
4253 1030 568 530 380 334 236 221
135 114 90 52 24
BbeI G_GCGC'C
Cuts at: 5401 5649 5401
Size: 248 7719
BbsI GAAGACnn'nnnn_
Cuts at: 740 2735 2738 740
Size: 1995 3 5969
Fragments arranged by size:
5969 1995 3
BbvCI CC'TCA_GC
Cuts at: 962 1183 962
Size: 221 7746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4940 4974 4940
Size: 34 7933
BciVI GTATCCnnnnn_n'
Cuts at: 3347 5865 6626 7967 3347
Size: 2518 761 1341 3347
Fragments arranged by size:
3347 2518 1341 761
BclI T'GATC_A
Cuts at: 3323 4604 5487 3323
Size: 1281 883 5803
Fragments arranged by size:
5803 1281 883
BfrBI ATG'CAT
Cuts at: 3243 3243
Size: 7967
BglI GCCn_nnn'nGGC
Cuts at: 4364 4364
Size: 7967
BglII A'GATC_T
Cuts at: 3848 5482 7519 3848
Size: 1634 2037 4296
Fragments arranged by size:
4296 2037 1634
BlpI GC'TnA_GC
Cuts at: 4214 5079 4214
Size: 865 7102
Bme1580I G_kGCm'C
Cuts at: 2278 4754 5592 5685 6741 2278
Size: 2476 838 93 1056 3504
Fragments arranged by size:
3504 2476 1056 838 93
BmgBI CAC'GTC
Cuts at: 4098 4098
Size: 7967
BmrI ACTGGGnnnn_n'
Cuts at: 479 1575 2181 2237 3396 5179 5260 5595 7739
Size: 1096 606 56 1159 1783 81 335 2144
Cuts at: 7739 479
Size: 707
Fragments arranged by size:
2144 1783 1159 1096 707 606 335 81
56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 2313 6334 738
Size: 1575 4021 2371
Fragments arranged by size:
4021 2371 1575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 369 598 851 1029 1282 1405 1744 2522
Size: 56 229 253 178 253 123 339 778
Cuts at: 2522 2999 4279 4390 4471 313
Size: 477 1280 111 81 3809
Fragments arranged by size:
3809 1280 778 477 339 253 253 229
178 123 111 81 56
Bpu10I CC'TnA_GC
Cuts at: 179 752 962 986 1183 179
Size: 573 210 24 197 6963
Fragments arranged by size:
6963 573 210 197 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 2661 4166 5143 6022 6514 6812 1303
Size: 1358 1505 977 879 492 298 2458
Fragments arranged by size:
2458 1505 1358 977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1062 1270 1594 2080 2500 2574 7671 1062
Size: 208 324 486 420 74 5097 1358
Fragments arranged by size:
5097 1358 486 420 324 208 74
BsaAI yAC'GTr
Cuts at: 1542 5320 1542
Size: 3778 4189
BsaBI GATnn'nnATC
Cuts at: 6 1850 5481 7518 6
Size: 1844 3631 2037 455
Fragments arranged by size:
3631 2037 1844 455
BsaHI Gr'CG_yC
Cuts at: 2752 4469 5398 5646 6348 7844 2752
Size: 1717 929 248 702 1496 2875
Fragments arranged by size:
2875 1717 1496 929 702 248
BsaWI w'CCGG_w
Cuts at: 245 1658 3120 4145 5156 5677 6355 6629 6776
Size: 1413 1462 1025 1011 521 678 274 147
Cuts at: 6776 7187 245
Size: 411 1025
Fragments arranged by size:
1462 1413 1025 1025 1011 678 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1024 1054 1543 1573 1024
Size: 30 489 30 7418
Fragments arranged by size:
7418 489 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1171 1279 1355 1716 2011 2305 2682 3600 4403
Size: 108 76 361 295 294 377 918 803
Cuts at: 4403 1171
Size: 4735
Fragments arranged by size:
4735 918 803 377 361 295 294 108
76
BseYI C'CCAG_C
Cuts at: 903 1683 1794 1941 2992 3592 4226 4334 4905
Size: 780 111 147 1051 600 634 108 571
Cuts at: 4905 5392 6150 6727 7223 903
Size: 487 758 577 496 1647
Fragments arranged by size:
1647 1051 780 758 634 600 577 571
496 487 147 111 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 3145 3826 544
Size: 2601 681 4685
Fragments arranged by size:
4685 2601 681
BsiEI CG_ry'CG
Cuts at: 104 1328 2829 3602 5509 5555 6763 104
Size: 1224 1501 773 1907 46 1208 1308
Fragments arranged by size:
1907 1501 1308 1224 1208 773 46
BsiHKAI G_wGCw'C
Cuts at: 299 1045 1139 2250 2789 2812 2864 2888 3464
Size: 746 94 1111 539 23 52 24 576
Cuts at: 3464 3677 3881 4981 5759 5949 6741 299
Size: 213 204 1100 778 190 792 1525
Fragments arranged by size:
1525 1111 1100 792 778 746 576 539
213 204 190 94 52 24 23
BsmBI CGTCTCn'nnnn_
Cuts at: 4104 4125 4936 4941 4104
Size: 21 811 5 7130
Fragments arranged by size:
7130 811 21 5
Bsp1286I G_dGCh'C
Cuts at: 299 1045 1139 2250 2278 2297 2677 2789 2812
Size: 746 94 1111 28 19 380 112 23
Cuts at: 2812 2864 2888 3456 3464 3677 3791 3881 4215
Size: 52 24 568 8 213 114 90 334
Cuts at: 4215 4745 4754 4981 5592 5685 5759 5949 6011
Size: 530 9 227 611 93 74 190 62
Cuts at: 6011 6741 299
Size: 730 1525
Fragments arranged by size:
1525 1111 746 730 611 568 530 380
334 227 213 190 114 112 94 93
90 74 62 52 28 24 23 19
9 8
BspEI T'CCGG_A
Cuts at: 1658 4145 7187 1658
Size: 2487 3042 2438
Fragments arranged by size:
3042 2487 2438
BspHI T'CATG_A
Cuts at: 7143 7143
Size: 7967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1747 3848 4682 4933 5017 5231 5533 5914 1747
Size: 2101 834 251 84 214 302 381 3800
Fragments arranged by size:
3800 2101 834 381 302 251 214 84
BsrBI CCG'CTC
Cuts at: 50 2831 3621 6259 6313 7797 7962 50
Size: 2781 790 2638 54 1484 165 55
Fragments arranged by size:
2781 2638 1484 790 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3587 5879 3587
Size: 2292 5675
BsrFI r'CCGG_y
Cuts at: 1058 1323 2386 2906 3120 4230 4363 4520 5965
Size: 265 1063 520 214 1110 133 157 1445
Cuts at: 5965 6146 6355 1058
Size: 181 209 2670
Fragments arranged by size:
2670 1445 1110 1063 520 265 214 209
181 157 133
BsrGI T'GTAC_A
Cuts at: 4682 4682
Size: 7967
BssSI C'ACGA_G
Cuts at: 155 883 1575 2797 6238 6596 155
Size: 728 692 1222 3441 358 1526
Fragments arranged by size:
3441 1526 1222 728 692 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5344 5344
Size: 7967
BstBI TT'CG_AA
Cuts at: 1779 6328 1779
Size: 4549 3418
BstEII G'GTnAC_C
Cuts at: 3198 3198
Size: 7967
BstXI CCAn_nnnn'nTGG
Cuts at: 1152 1260 2229 4242 1152
Size: 108 969 2013 4877
Fragments arranged by size:
4877 2013 969 108
BstYI r'GATC_y
Cuts at: 1 1553 3274 3659 3848 4691 4992 5459 5482
Size: 1552 1721 385 189 843 301 467 23
Cuts at: 5482 5817 6063 7075 7161 7173 7519 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1721 1552 1012 843 467 449 385 346
335 301 246 189 86 23 12
BtgI C'CryG_G
Cuts at: 107 358 547 1246 1301 1364 1547 1732 3729
Size: 251 189 699 55 63 183 185 1997
Cuts at: 3729 3941 4700 107
Size: 212 759 3374
Fragments arranged by size:
3374 1997 759 699 251 212 189 185
183 63 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 725 4011 5917 6098 6261 725
Size: 3286 1906 181 163 2431
Fragments arranged by size:
3286 2431 1906 181 163
BtsI GCAGTG_nn'
Cuts at: 1213 2781 3755 5210 1213
Size: 1568 974 1455 3970
Fragments arranged by size:
3970 1568 1455 974
DraI TTT'AAA
Cuts at: 3006 4966 3006
Size: 1960 6007
DraIII CAC_nnn'GTG
Cuts at: 1650 3404 4716 1650
Size: 1754 1312 4901
Fragments arranged by size:
4901 1754 1312
DrdI GACnn_nn'nnGTC
Cuts at: 3244 3994 5673 6531 3244
Size: 750 1679 858 4680
Fragments arranged by size:
4680 1679 858 750
EaeI y'GGCC_r
Cuts at: 1325 1811 3599 4232 4518 4697 5552 5726 6117
Size: 486 1788 633 286 179 855 174 391
Cuts at: 6117 6144 7489 1325
Size: 27 1345 1803
Fragments arranged by size:
1803 1788 1345 855 633 486 391 286
179 174 27
EagI C'GGCC_G
Cuts at: 1325 3599 5552 1325
Size: 2274 1953 3740
Fragments arranged by size:
3740 2274 1953
EarI CTCTTCn'nnn_
Cuts at: 867 1268 1975 3569 4198 4774 5990 6200 867
Size: 401 707 1594 629 576 1216 210 2634
Fragments arranged by size:
2634 1594 1216 707 629 576 401 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3994 4780 6175 6485 6631 7777 3994
Size: 786 1395 310 146 1146 4184
Fragments arranged by size:
4184 1395 1146 786 310 146
EcoICRI GAG'CTC
Cuts at: 2810 2862 2886 3675 3879 4979 2810
Size: 52 24 789 204 1100 5798
Fragments arranged by size:
5798 1100 789 204 52 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 313 369 470 598 851 1029 1282 1405
Size: 96 56 101 128 253 178 253 123
Cuts at: 1405 1744 2522 2999 3727 4279 4390 4471 5792
Size: 339 778 477 728 552 111 81 1321
Cuts at: 5792 6224 6971 7239 217
Size: 432 747 268 945
Fragments arranged by size:
1321 945 778 747 728 552 477 432
339 268 253 253 178 128 123 111
101 96 81 56
EcoO109I rG'GnC_Cy
Cuts at: 273 762 1938 2497 3227 5106 273
Size: 489 1176 559 730 1879 3134
Fragments arranged by size:
3134 1879 1176 730 559 489
EcoRI G'AATT_C
Cuts at: 2154 4971 2154
Size: 2817 5150
EcoRV GAT'ATC
Cuts at: 1656 3159 1656
Size: 1503 6464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1297 5986 1297
Size: 4689 3278
FspI TGC'GCA
Cuts at: 5748 5748
Size: 7967
HaeII r_GCGC'y
Cuts at: 501 1180 2655 3036 3829 4959 5401 5649 6671
Size: 679 1475 381 793 1130 442 248 1022
Cuts at: 6671 7650 501
Size: 979 818
Fragments arranged by size:
1475 1130 1022 979 818 793 679 442
381 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 727 759 1299 1331 1468 1500 1543
Size: 32 361 32 540 32 137 32 43
Cuts at: 1543 1575 2167 2199 2725 2757 2959 2991 3037
Size: 32 592 32 526 32 202 32 46
Cuts at: 3037 3062 3069 3094 3461 3493 3664 3696 4382
Size: 25 7 25 367 32 171 32 686
Cuts at: 4382 4414 4566 4598 5809 5839 5841 5871 334
Size: 32 152 32 1211 30 2 30 2430
Fragments arranged by size:
2430 1211 686 592 540 526 367 361
202 171 152 137 46 43 32 32
32 32 32 32 32 32 32 32
32 32 30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 327 1698 1890 1965 2568 4902 5006 327
Size: 1371 192 75 603 2334 104 3288
Fragments arranged by size:
3288 2334 1371 603 192 104 75
HindIII A'AGCT_T
Cuts at: 1405 1627 1879 2116 1405
Size: 222 252 237 7256
Fragments arranged by size:
7256 252 237 222
KasI G'GCGC_C
Cuts at: 5397 5645 5397
Size: 248 7719
KpnI G_GTAC'C
Cuts at: 4987 4987
Size: 7967
MfeI C'AATT_G
Cuts at: 283 283
Size: 7967
MluI A'CGCG_T
Cuts at: 7400 7400
Size: 7967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1586 2331 2876 4388 6638 6822 1586
Size: 745 545 1512 2250 184 2731
Fragments arranged by size:
2731 2250 1512 745 545 184
MscI TGG'CCA
Cuts at: 1813 4699 5728 1813
Size: 2886 1029 4052
Fragments arranged by size:
4052 2886 1029
MslI CAynn'nnrTG
Cuts at: 419 883 1150 1540 2227 2612 3385 3940 5956
Size: 464 267 390 687 385 773 555 2016
Cuts at: 5956 6083 419
Size: 127 2303
Fragments arranged by size:
2303 2016 773 687 555 464 390 385
267 127
MspA1I CmG'CkG
Cuts at: 360 1764 1794 1843 1945 2057 2095 3747 4218
Size: 1404 30 49 102 112 38 1652 471
Cuts at: 4218 4290 4334 5078 5392 5752 6765 7010 360
Size: 72 44 744 314 360 1013 245 1317
Fragments arranged by size:
1652 1404 1317 1013 744 471 360 314
245 112 102 72 49 44 38 30
NaeI GCC'GGC
Cuts at: 1060 1325 2908 4232 4522 6148 1060
Size: 265 1583 1324 290 1626 2879
Fragments arranged by size:
2879 1626 1583 1324 290 265
NarI GG'CG_CC
Cuts at: 5398 5646 5398
Size: 248 7719
NcoI C'CATG_G
Cuts at: 107 1246 1364 1547 3941 4700 107
Size: 1139 118 183 2394 759 3374
Fragments arranged by size:
3374 2394 1139 759 183 118
NdeI CA'TA_TG
Cuts at: 3956 3956
Size: 7967
NgoMIV G'CCGG_C
Cuts at: 1058 1323 2906 4230 4520 6146 1058
Size: 265 1583 1324 290 1626 2879
Fragments arranged by size:
2879 1626 1583 1324 290 265
NsiI A_TGCA'T
Cuts at: 3245 3245
Size: 7967
NspI r_CATG'y
Cuts at: 2241 3243 3247 4530 4739 5020 5520 6427 2241
Size: 1002 4 1283 209 281 500 907 3781
Fragments arranged by size:
3781 1283 1002 907 500 281 209 4
PciI A'CATG_T
Cuts at: 4526 4735 6423 4526
Size: 209 1688 6070
Fragments arranged by size:
6070 1688 209
PflMI CCAn_nnn'nTGG
Cuts at: 1365 1650 2043 2313 3178 4716 1365
Size: 285 393 270 865 1538 4616
Fragments arranged by size:
4616 1538 865 393 285 270
PfoI T'CCnGG_A
Cuts at: 2499 2746 2876 3662 3901 2499
Size: 247 130 786 239 6565
Fragments arranged by size:
6565 786 247 239 130
PmeI GTTT'AAAC
Cuts at: 4966 4966
Size: 7967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 2031 7106 7138 1999
Size: 32 5075 32 2828
Fragments arranged by size:
5075 2828 32 32
PpuMI rG'GwC_Cy
Cuts at: 273 762 2497 3227 273
Size: 489 1735 730 5013
Fragments arranged by size:
5013 1735 730 489
PshAI GACnn'nnGTC
Cuts at: 1074 1074
Size: 7967
PstI C_TGCA'G
Cuts at: 1172 1220 2894 3569 5014 5699 1172
Size: 48 1674 675 1445 685 3440
Fragments arranged by size:
3440 1674 1445 685 675 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7967
PvuII CAG'CTG
Cuts at: 1764 1794 1843 1945 2095 3747 4218 4334 5392
Size: 30 49 102 150 1652 471 116 1058
Cuts at: 5392 5752 1764
Size: 360 3979
Fragments arranged by size:
3979 1652 1058 471 360 150 116 102
49 30
RsrII CG'GwC_CG
Cuts at: 6162 6162
Size: 7967
SacI G_AGCT'C
Cuts at: 2812 2864 2888 3677 3881 4981 2812
Size: 52 24 789 204 1100 5798
Fragments arranged by size:
5798 1100 789 204 52 24
SacII CC_GC'GG
Cuts at: 361 361
Size: 7967
SalI G'TCGA_C
Cuts at: 5004 5004
Size: 7967
SapI GCTCTTCn'nnn_
Cuts at: 867 1975 5990 6200 867
Size: 1108 4015 210 2634
Fragments arranged by size:
4015 2634 1108 210
SbfI CC_TGCA'GG
Cuts at: 3569 5014 3569
Size: 1445 6522
ScaI AGT'ACT
Cuts at: 3889 7484 3889
Size: 3595 4372
SexAI A'CCwGG_T
Cuts at: 2737 4712 2737
Size: 1975 5992
SfcI C'TryA_G
Cuts at: 33 1168 1216 2890 3565 5010 5695 6688 6879
Size: 1135 48 1674 675 1445 685 993 191
Cuts at: 6879 33
Size: 1121
Fragments arranged by size:
1674 1445 1135 1121 993 685 675 191
48
SfiI GGCCn_nnn'nGGCC
Cuts at: 4364 4364
Size: 7967
SfoI GGC'GCC
Cuts at: 5399 5647 5399
Size: 248 7719
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7967
SmaI CCC'GGG
Cuts at: 2352 2867 4989 2352
Size: 515 2122 5330
Fragments arranged by size:
5330 2122 515
SmlI C'TyrA_G
Cuts at: 459 1282 2676 3505 4181 5122 6037 6529 6791
Size: 823 1394 829 676 941 915 492 262
Cuts at: 6791 459
Size: 1635
Fragments arranged by size:
1635 1394 941 915 829 823 676 492
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7967
SphI G_CATG'C
Cuts at: 5020 5520 5020
Size: 500 7467
SspI AAT'ATT
Cuts at: 227 2200 3101 3165 4419 227
Size: 1973 901 64 1254 3775
Fragments arranged by size:
3775 1973 1254 901 64
StuI AGG'CCT
Cuts at: 2528 3433 4034 2528
Size: 905 601 6461
Fragments arranged by size:
6461 905 601
StyI C'CwwG_G
Cuts at: 107 1246 1364 1472 1547 2573 3110 3428 3941
Size: 1139 118 108 75 1026 537 318 513
Cuts at: 3941 4700 5101 5455 107
Size: 759 401 354 2619
Fragments arranged by size:
2619 1139 1026 759 537 513 401 354
318 118 108 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1572 1766 2050 2221 2524 3415 3646 5550 5683
Size: 194 284 171 303 891 231 1904 133
Cuts at: 5683 6142 6351 1572
Size: 459 209 3188
Fragments arranged by size:
3188 1904 891 459 303 284 231 209
194 171 133
TatI w'GTAC_w
Cuts at: 3887 4682 7482 3887
Size: 795 2800 4372
Fragments arranged by size:
4372 2800 795
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1024 1056 4099 4131 7484 7516 1024
Size: 32 3043 32 3353 32 1475
Fragments arranged by size:
3353 3043 1475 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2482 2754 5764 2482
Size: 272 3010 4685
Fragments arranged by size:
4685 3010 272
XbaI T'CTAG_A
Cuts at: 4998 4998
Size: 7967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 2044 2426 3939 5462 1244
Size: 800 382 1513 1523 3749
Fragments arranged by size:
3749 1523 1513 800 382
XhoI C'TCGA_G
Cuts at: 3505 3505
Size: 7967
XmaI C'CCGG_G
Cuts at: 2350 2865 4987 2350
Size: 515 2122 5330
Fragments arranged by size:
5330 2122 515
XmnI GAAnn'nnTTC
Cuts at: 2154 2154
Size: 7967
ZraI GAC'GTC
Cuts at: 4470 4470
Size: 7967
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AlfI AloI ApaI AscI AvrII BaeI BmtI BsiWI
BsmI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI NheI NotI NruI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SanDI SgrAI SnaBI SrfI
SwaI