Restriction map for
pF1KA0560
(Circular) (Six-base) MAPSORT of: pF1KA0560.seq Check: 4428 from: 1 to: 7568
pF1KA0560 7568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4278 4278
Size: 7568
AccI GT'mk_AC
Cuts at: 584 4202 4606 584
Size: 3618 404 3546
Fragments arranged by size:
3618 3546 404
Acc65I G'GTAC_C
Cuts at: 2154 4584 2154
Size: 2430 5138
AclI AA'CG_TT
Cuts at: 7408 7408
Size: 7568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3110 5393 5825 6178 6840 3110
Size: 2283 432 353 662 3838
Fragments arranged by size:
3838 2283 662 432 353
AflIII A'CryG_T
Cuts at: 1923 3746 4208 4920 6720 7001 1923
Size: 1823 462 712 1800 281 2490
Fragments arranged by size:
2490 1823 1800 712 462 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3552 3552
Size: 7568
AlwNI CAG_nnn'CTG
Cuts at: 118 1889 3309 4873 6311 118
Size: 1771 1420 1564 1438 1375
Fragments arranged by size:
1771 1564 1438 1420 1375
ApaI G_GGCC'C
Cuts at: 2578 2578
Size: 7568
ApaLI G'TGCA_C
Cuts at: 2204 6406 2204
Size: 4202 3366
ApoI r'AATT_y
Cuts at: 310 517 691 1024 1309 1349 1473 1547 1895
Size: 207 174 333 285 40 124 74 348
Cuts at: 1895 3116 3136 4572 6921 7177 310
Size: 1221 20 1436 2349 256 701
Fragments arranged by size:
2349 1436 1221 701 348 333 285 256
207 174 124 74 40 20
AseI AT'TA_AT
Cuts at: 20 1046 3647 20
Size: 1026 2601 3941
Fragments arranged by size:
3941 2601 1026
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7568
AvaI C'yCGr_G
Cuts at: 1190 1706 4588 1190
Size: 516 2882 4170
Fragments arranged by size:
4170 2882 516
BamHI G'GATC_C
Cuts at: 729 1995 4593 729
Size: 1266 2598 3704
Fragments arranged by size:
3704 2598 1266
BanI G'GyrC_C
Cuts at: 2154 4584 4998 5246 5281 2154
Size: 2430 414 248 35 4441
Fragments arranged by size:
4441 2430 414 248 35
BanII G_rGCy'C
Cuts at: 204 2450 2578 4582 5612 204
Size: 2246 128 2004 1030 2160
Fragments arranged by size:
2246 2160 2004 1030 128
BbeI G_GCGC'C
Cuts at: 5002 5250 5002
Size: 248 7320
BbsI GAAGACnn'nnnn_
Cuts at: 1295 1765 2327 2420 3080 3870 1295
Size: 470 562 93 660 790 4993
Fragments arranged by size:
4993 790 660 562 470 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2195 2229 3799 3833 2195
Size: 34 1570 34 5930
Fragments arranged by size:
5930 1570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1397 4038 5466 6522 7568 1397
Size: 2641 1428 1056 1046 1397
Fragments arranged by size:
2641 1428 1397 1056 1046
BclI T'GATC_A
Cuts at: 558 5088 558
Size: 4530 3038
BfrBI ATG'CAT
Cuts at: 4218 4218
Size: 7568
BglII A'GATC_T
Cuts at: 3061 3175 5083 7120 3061
Size: 114 1908 2037 3509
Fragments arranged by size:
3509 2037 1908 114
BlpI GC'TnA_GC
Cuts at: 4680 4680
Size: 7568
Bme1580I G_kGCm'C
Cuts at: 2208 2578 5193 5286 6410 2208
Size: 370 2615 93 1124 3366
Fragments arranged by size:
3366 2615 1124 370 93
BmgBI CAC'GTC
Cuts at: 1924 1924
Size: 7568
BmrI ACTGGGnnnn_n'
Cuts at: 174 207 4358 4418 4780 4861 5196 7340 174
Size: 33 4151 60 362 81 335 2144 402
Fragments arranged by size:
4151 2144 402 362 335 81 60 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4048 4169 5935 4048
Size: 121 1766 5681
Fragments arranged by size:
5681 1766 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 2137 2785 3331 3395 4043 4382 4433 1463
Size: 674 648 546 64 648 339 51 4598
Fragments arranged by size:
4598 674 648 648 546 339 64 51
Bpu10I CC'TnA_GC
Cuts at: 680 1507 680
Size: 827 6741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3151 3497 4744 5623 6337 6635 3151
Size: 346 1247 879 714 298 4084
Fragments arranged by size:
4084 1247 879 714 346 298
BsaI GGTCTCn'nnnn_
Cuts at: 1842 4140 4468 4549 7272 1842
Size: 2298 328 81 2723 2138
Fragments arranged by size:
2723 2298 2138 328 81
BsaAI yAC'GTr
Cuts at: 4921 4921
Size: 7568
BsaBI GATnn'nnATC
Cuts at: 6 992 5082 7119 6
Size: 986 4090 2037 455
Fragments arranged by size:
4090 2037 986 455
BsaHI Gr'CG_yC
Cuts at: 4999 5247 5949 7445 4999
Size: 248 702 1496 5122
Fragments arranged by size:
5122 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1841 4757 5278 5956 6367 6514 6788 1841
Size: 2916 521 678 411 147 274 2621
Fragments arranged by size:
2916 2621 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4431 4461 4431
Size: 30 7538
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 777 1860 1979 2094 3029 777
Size: 1083 119 115 935 5316
Fragments arranged by size:
5316 1083 935 119 115
BseYI C'CCAG_C
Cuts at: 1004 1640 2936 4993 5751 6416 6824 1004
Size: 636 1296 2057 758 665 408 1748
Fragments arranged by size:
2057 1748 1296 758 665 636 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2152 3607 4490 1736
Size: 416 1455 883 4814
Fragments arranged by size:
4814 1455 883 416
BsiEI CG_ry'CG
Cuts at: 104 5110 5156 6386 104
Size: 5006 46 1230 1286
Fragments arranged by size:
5006 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2208 4582 5360 5550 6410 2208
Size: 2374 778 190 860 3366
Fragments arranged by size:
3366 2374 860 778 190
BsiWI C'GTAC_G
Cuts at: 1806 1806
Size: 7568
BsmI GAATG_Cn'
Cuts at: 2552 2552
Size: 7568
Bsp1286I G_dGCh'C
Cuts at: 204 2208 2450 2578 4582 5193 5286 5360 5550
Size: 2004 242 128 2004 611 93 74 190
Cuts at: 5550 5612 6410 204
Size: 62 798 1362
Fragments arranged by size:
2004 2004 1362 798 611 242 190 128
93 74 62
BspHI T'CATG_A
Cuts at: 528 1338 1494 4155 6000 528
Size: 810 156 2661 1845 2096
Fragments arranged by size:
2661 2096 1845 810 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1511 1854 2777 4278 4618 4832 5134 5515 1511
Size: 343 923 1501 340 214 302 381 3564
Fragments arranged by size:
3564 1501 923 381 343 340 302 214
BsrBI CCG'CTC
Cuts at: 50 5860 5914 7398 7563 50
Size: 5810 54 1484 165 55
Fragments arranged by size:
5810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 561 2977 5480 561
Size: 2416 2503 2649
Fragments arranged by size:
2649 2503 2416
BsrFI r'CCGG_y
Cuts at: 4509 5566 5747 4509
Size: 1057 181 6330
Fragments arranged by size:
6330 1057 181
BsrGI T'GTAC_A
Cuts at: 3743 4211 3743
Size: 468 7100
BssSI C'ACGA_G
Cuts at: 5839 6547 5839
Size: 708 6860
BstAPI GCAn_nnn'nTGC
Cuts at: 118 3594 4945 118
Size: 3476 1351 2741
Fragments arranged by size:
3476 2741 1351
BstBI TT'CG_AA
Cuts at: 2215 3159 5929 2215
Size: 944 2770 3854
Fragments arranged by size:
3854 2770 944
BstEII G'GTnAC_C
Cuts at: 3760 3760
Size: 7568
BstXI CCAn_nnnn'nTGG
Cuts at: 3948 3948
Size: 7568
BstYI r'GATC_y
Cuts at: 1 729 1995 3061 3175 4593 5060 5083 5418
Size: 728 1266 1066 114 1418 467 23 335
Cuts at: 5418 5664 5970 5982 6068 7120 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1418 1266 1066 1052 728 467 449 335
306 246 114 86 23 12
BstZ17I GTA'TAC
Cuts at: 4203 4203
Size: 7568
Bsu36I CC'TnA_GG
Cuts at: 356 3350 4529 356
Size: 2994 1179 3395
Fragments arranged by size:
3395 2994 1179
BtgI C'CryG_G
Cuts at: 107 1588 107
Size: 1481 6087
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5518 5699 5862 5518
Size: 181 163 7224
Fragments arranged by size:
7224 181 163
BtsI GCAGTG_nn'
Cuts at: 3953 4811 3953
Size: 858 6710
DraI TTT'AAA
Cuts at: 477 2279 3054 4567 477
Size: 1802 775 1513 3478
Fragments arranged by size:
3478 1802 1513 775
DraIII CAC_nnn'GTG
Cuts at: 1245 1245
Size: 7568
DrdI GACnn_nn'nnGTC
Cuts at: 5274 6618 5274
Size: 1344 6224
EaeI y'GGCC_r
Cuts at: 990 1673 1699 3801 3983 5153 5327 5718 5745
Size: 683 26 2102 182 1170 174 391 27
Cuts at: 5745 7090 990
Size: 1345 1468
Fragments arranged by size:
2102 1468 1345 1170 683 391 182 174
27 26
EagI C'GGCC_G
Cuts at: 5153 5153
Size: 7568
EarI CTCTTCn'nnn_
Cuts at: 977 1280 1466 1496 1528 2753 2790 2936 3287
Size: 303 186 30 32 1225 37 146 351
Cuts at: 3287 3338 3469 4039 4277 4325 4547 5591 5801
Size: 51 131 570 238 48 222 1044 210
Cuts at: 5801 977
Size: 2744
Fragments arranged by size:
2744 1225 1044 570 351 303 238 222
210 186 146 131 51 48 37 32
30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5776 6518 6664 7378 5776
Size: 742 146 714 5966
Fragments arranged by size:
5966 742 714 146
EcoICRI GAG'CTC
Cuts at: 4580 4580
Size: 7568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 2137 2785 3110 3331 3395 4043 4382 4433
Size: 674 648 325 221 64 648 339 51
Cuts at: 4433 5393 5825 6178 6840 1463
Size: 960 432 353 662 2191
Fragments arranged by size:
2191 960 674 662 648 648 432 353
339 325 221 64 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 7568
EcoO109I rG'GnC_Cy
Cuts at: 891 2193 2470 2679 4707 891
Size: 1302 277 209 2028 3752
Fragments arranged by size:
3752 2028 1302 277 209
EcoRI G'AATT_C
Cuts at: 3136 4572 3136
Size: 1436 6132
EcoRV GAT'ATC
Cuts at: 2175 2175
Size: 7568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1330 1471 5587 1330
Size: 141 4116 3311
Fragments arranged by size:
4116 3311 141
FspI TGC'GCA
Cuts at: 122 5349 122
Size: 5227 2341
HaeII r_GCGC'y
Cuts at: 912 2730 5002 5250 6480 7251 912
Size: 1818 2272 248 1230 771 1229
Fragments arranged by size:
2272 1818 1230 1229 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 582 614 1736 1768 3210 3242 3378 3410 3998
Size: 32 1122 32 1442 32 136 32 588
Cuts at: 3998 4030 4135 4167 4221 4253 4525 4557 5410
Size: 32 105 32 54 32 272 32 853
Cuts at: 5410 5440 5442 5472 582
Size: 30 2 30 2678
Fragments arranged by size:
2678 1442 1122 853 588 272 136 105
54 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3501 3904 4607 3501
Size: 403 703 6462
Fragments arranged by size:
6462 703 403
HindIII A'AGCT_T
Cuts at: 3531 3531
Size: 7568
KasI G'GCGC_C
Cuts at: 4998 5246 4998
Size: 248 7320
KpnI G_GTAC'C
Cuts at: 2158 4588 2158
Size: 2430 5138
MluI A'CGCG_T
Cuts at: 7001 7001
Size: 7568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2645 3465 3517 4132 4393 6327 6511 1414
Size: 1231 820 52 615 261 1934 184 2471
Fragments arranged by size:
2471 1934 1231 820 615 261 184 52
MscI TGG'CCA
Cuts at: 992 1675 1701 3803 3985 5329 992
Size: 683 26 2102 182 1344 3231
Fragments arranged by size:
3231 2102 1344 683 182 26
MslI CAynn'nnrTG
Cuts at: 854 3286 4213 5557 5684 854
Size: 2432 927 1344 127 2738
Fragments arranged by size:
2738 2432 1344 927 127
MspA1I CmG'CkG
Cuts at: 645 4382 4679 4993 5353 6137 6382 645
Size: 3737 297 314 360 784 245 1831
Fragments arranged by size:
3737 1831 784 360 314 297 245
NaeI GCC'GGC
Cuts at: 5749 5749
Size: 7568
NarI GG'CG_CC
Cuts at: 4999 5247 4999
Size: 248 7320
NcoI C'CATG_G
Cuts at: 107 1588 107
Size: 1481 6087
NgoMIV G'CCGG_C
Cuts at: 5747 5747
Size: 7568
NruI TCG'CGA
Cuts at: 3821 3821
Size: 7568
NsiI A_TGCA'T
Cuts at: 4220 4220
Size: 7568
NspI r_CATG'y
Cuts at: 191 1020 1908 3750 4212 4218 4621 5121 6724
Size: 829 888 1842 462 6 403 500 1603
Cuts at: 6724 191
Size: 1035
Fragments arranged by size:
1842 1603 1035 888 829 500 462 403
6
PciI A'CATG_T
Cuts at: 3746 4208 6720 3746
Size: 462 2512 4594
Fragments arranged by size:
4594 2512 462
PflMI CCAn_nnn'nTGG
Cuts at: 945 1011 1245 3859 945
Size: 66 234 2614 4654
Fragments arranged by size:
4654 2614 234 66
PfoI T'CCnGG_A
Cuts at: 926 2871 926
Size: 1945 5623
PmeI GTTT'AAAC
Cuts at: 4567 4567
Size: 7568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6014 6046 6014
Size: 32 7536
PpuMI rG'GwC_Cy
Cuts at: 2193 2470 2193
Size: 277 7291
PsiI TTA'TAA
Cuts at: 2483 2483
Size: 7568
PspOMI G'GGCC_C
Cuts at: 2574 2574
Size: 7568
PstI C_TGCA'G
Cuts at: 2904 4615 5300 2904
Size: 1711 685 5172
Fragments arranged by size:
5172 1711 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7568
PvuII CAG'CTG
Cuts at: 645 4382 4993 5353 645
Size: 3737 611 360 2860
Fragments arranged by size:
3737 2860 611 360
RsrII CG'GwC_CG
Cuts at: 5763 5763
Size: 7568
SacI G_AGCT'C
Cuts at: 4582 4582
Size: 7568
SalI G'TCGA_C
Cuts at: 4605 4605
Size: 7568
SanDI GG'GwC_CC
Cuts at: 2193 2193
Size: 7568
SapI GCTCTTCn'nnn_
Cuts at: 1466 2753 5591 5801 1466
Size: 1287 2838 210 3233
Fragments arranged by size:
3233 2838 1287 210
SbfI CC_TGCA'GG
Cuts at: 4615 4615
Size: 7568
ScaI AGT'ACT
Cuts at: 1435 3442 7085 1435
Size: 2007 3643 1918
Fragments arranged by size:
3643 2007 1918
SexAI A'CCwGG_T
Cuts at: 1241 2580 2921 1241
Size: 1339 341 5888
Fragments arranged by size:
5888 1339 341
SfcI C'TryA_G
Cuts at: 33 1117 2315 2900 3340 4611 5296 6264 6455
Size: 1084 1198 585 440 1271 685 968 191
Cuts at: 6455 33
Size: 1146
Fragments arranged by size:
1271 1198 1146 1084 968 685 585 440
191
SfoI GGC'GCC
Cuts at: 5000 5248 5000
Size: 248 7320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7568
SmaI CCC'GGG
Cuts at: 4590 4590
Size: 7568
SmlI C'TyrA_G
Cuts at: 3130 3512 4723 5638 6352 6614 3130
Size: 382 1211 915 714 262 4084
Fragments arranged by size:
4084 1211 915 714 382 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7568
SphI G_CATG'C
Cuts at: 1908 4621 5121 1908
Size: 2713 500 4355
Fragments arranged by size:
4355 2713 500
SspI AAT'ATT
Cuts at: 1419 1419
Size: 7568
StuI AGG'CCT
Cuts at: 373 373
Size: 7568
StyI C'CwwG_G
Cuts at: 107 1199 1588 4559 4702 5056 107
Size: 1092 389 2971 143 354 2619
Fragments arranged by size:
2971 2619 1092 389 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5151 5284 5743 5952 5151
Size: 133 459 209 6767
Fragments arranged by size:
6767 459 209 133
TatI w'GTAC_w
Cuts at: 603 1433 2974 3223 3440 3607 3743 4161 4211
Size: 830 1541 249 217 167 136 418 50
Cuts at: 4211 7083 603
Size: 2872 1088
Fragments arranged by size:
2872 1541 1088 830 418 249 217 167
136 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 767 799 4431 4463 7085 7117 767
Size: 32 3632 32 2622 32 1218
Fragments arranged by size:
3632 2622 1218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 5365 3253
Size: 2112 5456
XbaI T'CTAG_A
Cuts at: 885 1317 3991 4599 885
Size: 432 2674 608 3854
Fragments arranged by size:
3854 2674 608 432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 135 4485 5063 135
Size: 4350 578 2640
Fragments arranged by size:
4350 2640 578
XmaI C'CCGG_G
Cuts at: 4588 4588
Size: 7568
XmnI GAAnn'nnTTC
Cuts at: 1110 1313 1772 2779 3155 3659 1110
Size: 203 459 1007 376 504 5019
Fragments arranged by size:
5019 1007 504 459 376 203
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbvCI BglI BmtI BsmBI BspEI BssHII
ClaI CspCI FseI FspAI HpaI MfeI NdeI NheI
NotI PacI PasI PmlI PshAI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI