Restriction map for
pF1KA0561
(Circular) (Six-base) MAPSORT of: pF1KA0561.seq Check: 9884 from: 1 to: 7040
pF1KA0561 7040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1001 1713 3750 1001
Size: 712 2037 4291
Fragments arranged by size:
4291 2037 712
AatII G_ACGT'C
Cuts at: 3035 3164 3035
Size: 129 6911
AccI GT'mk_AC
Cuts at: 1250 2228 3014 3035 4078 1250
Size: 978 786 21 1043 4212
Fragments arranged by size:
4212 1043 978 786 21
Acc65I G'GTAC_C
Cuts at: 4056 4056
Size: 7040
AclI AA'CG_TT
Cuts at: 2127 6880 2127
Size: 4753 2287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2271 2976 3199 4865 5297 5650 6312 1495
Size: 776 705 223 1666 432 353 662 2223
Fragments arranged by size:
2223 1666 776 705 662 432 353 223
AfeI AGC'GCT
Cuts at: 1282 1282
Size: 7040
AflIII A'CryG_T
Cuts at: 629 2116 4392 6192 6473 629
Size: 1487 2276 1800 281 1196
Fragments arranged by size:
2276 1800 1487 1196 281
AgeI A'CCGG_T
Cuts at: 2213 2213
Size: 7040
AleI CACnn'nnGTG
Cuts at: 2575 3967 2575
Size: 1392 5648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1301 1301
Size: 7040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2881 2913 2881
Size: 32 7008
AlwNI CAG_nnn'CTG
Cuts at: 1144 2512 2728 2800 3487 3562 4345 5783 1144
Size: 1368 216 72 687 75 783 1438 2401
Fragments arranged by size:
2401 1438 1368 783 687 216 75 72
ApaI G_GGCC'C
Cuts at: 1489 2713 3244 4022 1489
Size: 1224 531 778 4507
Fragments arranged by size:
4507 1224 778 531
ApaLI G'TGCA_C
Cuts at: 1567 3043 3151 5878 1567
Size: 1476 108 2727 2729
Fragments arranged by size:
2729 2727 1476 108
ApoI r'AATT_y
Cuts at: 297 321 982 4044 6393 6649 297
Size: 24 661 3062 2349 256 688
Fragments arranged by size:
3062 2349 688 661 256 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 7040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7040
AvaI C'yCGr_G
Cuts at: 434 579 773 1703 1753 1861 2413 2592 3531
Size: 145 194 930 50 108 552 179 939
Cuts at: 3531 3724 4015 4060 434
Size: 193 291 45 3414
Fragments arranged by size:
3414 939 930 552 291 194 193 179
145 108 50 45
BamHI G'GATC_C
Cuts at: 2383 4065 2383
Size: 1682 5358
BanI G'GyrC_C
Cuts at: 131 709 1104 1262 1418 1996 2653 3728 4056
Size: 578 395 158 156 578 657 1075 328
Cuts at: 4056 4470 4718 4753 131
Size: 414 248 35 2418
Fragments arranged by size:
2418 1075 657 578 578 414 395 328
248 158 156 35
BanII G_rGCy'C
Cuts at: 143 1489 1972 2146 2713 2863 3244 3538 3851
Size: 1346 483 174 567 150 381 294 313
Cuts at: 3851 4022 4054 5084 143
Size: 171 32 1030 2099
Fragments arranged by size:
2099 1346 1030 567 483 381 313 294
174 171 150 32
BbeI G_GCGC'C
Cuts at: 135 713 1422 2657 4474 4722 135
Size: 578 709 1235 1817 248 2453
Fragments arranged by size:
2453 1817 1235 709 578 248
BbsI GAAGACnn'nnnn_
Cuts at: 2300 2526 2300
Size: 226 6814
BbvCI CC'TCA_GC
Cuts at: 1007 2362 1007
Size: 1355 5685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 108 142 2596 2630 2631 2665 108
Size: 34 2454 34 1 34 4483
Fragments arranged by size:
4483 2454 34 34 34 1
BciVI GTATCCnnnnn_n'
Cuts at: 4938 5994 7040 4938
Size: 1056 1046 4938
Fragments arranged by size:
4938 1056 1046
BclI T'GATC_A
Cuts at: 926 4560 926
Size: 3634 3406
BglI GCCn_nnn'nGGC
Cuts at: 340 718 948 1243 2141 2516 2708 3088 3428
Size: 378 230 295 898 375 192 380 340
Cuts at: 3428 340
Size: 3952
Fragments arranged by size:
3952 898 380 378 375 340 295 230
192
BglII A'GATC_T
Cuts at: 3341 4555 6592 3341
Size: 1214 2037 3789
Fragments arranged by size:
3789 2037 1214
BlpI GC'TnA_GC
Cuts at: 154 4152 154
Size: 3998 3042
Bme1580I G_kGCm'C
Cuts at: 1489 1571 1990 2078 2713 2789 3047 3155 3244
Size: 82 419 88 635 76 258 108 89
Cuts at: 3244 3731 3793 4022 4665 4758 5882 1489
Size: 487 62 229 643 93 1124 2647
Fragments arranged by size:
2647 1124 643 635 487 419 258 229
108 93 89 88 82 76 62
BmgBI CAC'GTC
Cuts at: 175 3051 175
Size: 2876 4164
BmrI ACTGGGnnnn_n'
Cuts at: 2049 2187 2569 3962 4252 4333 4668 6812 2049
Size: 138 382 1393 290 81 335 2144 2277
Fragments arranged by size:
2277 2144 1393 382 335 290 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3243 5407 3243
Size: 2164 4876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 865 976 985 1018 1114 1561 2733 3078
Size: 738 111 9 33 96 447 1172 345
Cuts at: 3078 3238 3522 127
Size: 160 284 3645
Fragments arranged by size:
3645 1172 738 447 345 284 160 111
96 33 9
Bpu10I CC'TnA_GC
Cuts at: 1007 2362 1007
Size: 1355 5685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5095 5809 6107 4216
Size: 879 714 298 5149
Fragments arranged by size:
5149 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1204 1405 2152 2711 2915 3301 6744 1204
Size: 201 747 559 204 386 3443 1500
Fragments arranged by size:
3443 1500 747 559 386 204 201
BsaAI yAC'GTr
Cuts at: 630 1446 4393 630
Size: 816 2947 3277
Fragments arranged by size:
3277 2947 816
BsaBI GATnn'nnATC
Cuts at: 6 931 4554 6591 6
Size: 925 3623 2037 455
Fragments arranged by size:
3623 2037 925 455
BsaHI Gr'CG_yC
Cuts at: 132 469 710 734 1419 1700 1735 1808 1934
Size: 337 241 24 685 281 35 73 126
Cuts at: 1934 2654 3032 3161 4471 4719 5421 6917 132
Size: 720 378 129 1310 248 702 1496 255
Fragments arranged by size:
1496 1310 720 702 685 378 337 281
255 248 241 129 126 73 35 24
BsaWI w'CCGG_w
Cuts at: 914 2213 3388 4024 4229 4750 5428 5839 5986
Size: 1299 1175 636 205 521 678 411 147
Cuts at: 5986 6260 914
Size: 274 1694
Fragments arranged by size:
1694 1299 1175 678 636 521 411 274
205 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 2274 2293 2304 2323 3433 3463 536
Size: 30 1708 19 11 19 1110 30 4113
Fragments arranged by size:
4113 1708 1110 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 901 1111 2190 2317 2736 3306 3375 3958 901
Size: 210 1079 127 419 570 69 583 3983
Fragments arranged by size:
3983 1079 583 570 419 210 127 69
BseYI C'CCAG_C
Cuts at: 403 1071 1137 2078 2408 2425 2721 3103 3141
Size: 668 66 941 330 17 296 382 38
Cuts at: 3141 3454 3480 3495 3520 3555 3580 3786 4465
Size: 313 26 15 25 35 25 206 679
Cuts at: 4465 5223 5888 6296 403
Size: 758 665 408 1147
Fragments arranged by size:
1147 941 758 679 668 665 408 382
330 313 296 206 66 38 35 26
25 25 17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 3349 3574 3853 3934 466
Size: 2883 225 279 81 3572
Fragments arranged by size:
3572 2883 279 225 81
BsiEI CG_ry'CG
Cuts at: 104 540 2691 4582 4628 5858 104
Size: 436 2151 1891 46 1230 1286
Fragments arranged by size:
2151 1891 1286 1230 436 46
BsiHKAI G_wGCw'C
Cuts at: 1571 3047 3155 3538 3851 4054 4832 5022 5882
Size: 1476 108 383 313 203 778 190 860
Cuts at: 5882 1571
Size: 2729
Fragments arranged by size:
2729 1476 860 778 383 313 203 190
108
BsmBI CGTCTCn'nnnn_
Cuts at: 374 448 1519 4025 374
Size: 74 1071 2506 3389
Fragments arranged by size:
3389 2506 1071 74
Bsp1286I G_dGCh'C
Cuts at: 143 1489 1571 1972 1990 2078 2146 2713 2789
Size: 1346 82 401 18 88 68 567 76
Cuts at: 2789 2863 3047 3155 3244 3538 3731 3793 3851
Size: 74 184 108 89 294 193 62 58
Cuts at: 3851 4022 4054 4665 4758 4832 5022 5084 5882
Size: 171 32 611 93 74 190 62 798
Cuts at: 5882 143
Size: 1301
Fragments arranged by size:
1346 1301 798 611 567 401 294 193
190 184 171 108 93 89 88 82
76 74 74 68 62 62 58 32
18
BspEI T'CCGG_A
Cuts at: 914 3388 914
Size: 2474 4566
BspHI T'CATG_A
Cuts at: 617 1655 2855 5472 617
Size: 1038 1200 2617 2185
Fragments arranged by size:
2617 2185 1200 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1001 1329 1556 1713 3750 4090 4304 4606 4987
Size: 328 227 157 2037 340 214 302 381
Cuts at: 4987 1001
Size: 3054
Fragments arranged by size:
3054 2037 381 340 328 302 227 214
157
BsrBI CCG'CTC
Cuts at: 50 563 849 2418 3185 3707 3801 5332 5386
Size: 513 286 1569 767 522 94 1531 54
Cuts at: 5386 6870 7035 50
Size: 1484 165 55
Fragments arranged by size:
1569 1531 1484 767 522 513 286 165
94 55 54
BsrDI GCAATG_nn'
Cuts at: 4952 4952
Size: 7040
BsrFI r'CCGG_y
Cuts at: 539 2213 2339 2686 5038 5219 539
Size: 1674 126 347 2352 181 2360
Fragments arranged by size:
2360 2352 1674 347 181 126
BssSI C'ACGA_G
Cuts at: 3830 3850 5311 6019 3830
Size: 20 1461 708 4851
Fragments arranged by size:
4851 1461 708 20
BstAPI GCAn_nnn'nTGC
Cuts at: 1400 4417 1400
Size: 3017 4023
BstBI TT'CG_AA
Cuts at: 5401 5401
Size: 7040
BstEII G'GTnAC_C
Cuts at: 542 542
Size: 7040
BstXI CCAn_nnnn'nTGG
Cuts at: 1777 1793 2454 2974 3216 3237 1777
Size: 16 661 520 242 21 5580
Fragments arranged by size:
5580 661 520 242 21 16
BstYI r'GATC_y
Cuts at: 1 1331 2186 2383 3341 4065 4532 4555 4890
Size: 1330 855 197 958 724 467 23 335
Cuts at: 4890 5136 5442 5454 5540 6592 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1330 1052 958 855 724 467 449 335
306 246 197 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 977 977
Size: 7040
BtgI C'CryG_G
Cuts at: 107 135 1496 1802 3754 3983 107
Size: 28 1361 306 1952 229 3164
Fragments arranged by size:
3164 1952 1361 306 229 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1904 4990 5171 5334 1904
Size: 3086 181 163 3610
Fragments arranged by size:
3610 3086 181 163
BtsI GCAGTG_nn'
Cuts at: 2766 2778 3506 4283 2766
Size: 12 728 777 5523
Fragments arranged by size:
5523 777 728 12
DraI TTT'AAA
Cuts at: 4039 4039
Size: 7040
DraIII CAC_nnn'GTG
Cuts at: 2007 2007
Size: 7040
DrdI GACnn_nn'nnGTC
Cuts at: 4746 6090 4746
Size: 1344 5696
EaeI y'GGCC_r
Cuts at: 537 785 940 1079 1173 1611 1664 1903 2249
Size: 248 155 139 94 438 53 239 346
Cuts at: 2249 2334 2567 2688 3260 3567 3633 4625 4799
Size: 85 233 121 572 307 66 992 174
Cuts at: 4799 5190 5217 6562 537
Size: 391 27 1345 1015
Fragments arranged by size:
1345 1015 992 572 438 391 346 307
248 239 233 174 155 139 121 94
85 66 53 27
EagI C'GGCC_G
Cuts at: 537 2688 4625 537
Size: 2151 1937 2952
Fragments arranged by size:
2952 2151 1937
EarI CTCTTCn'nnn_
Cuts at: 1876 2913 3175 4023 5063 5273 1876
Size: 1037 262 848 1040 210 3643
Fragments arranged by size:
3643 1040 1037 848 262 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1756 3336 5248 5990 6136 6850 1756
Size: 1580 1912 742 146 714 1946
Fragments arranged by size:
1946 1912 1580 742 714 146
EcoICRI GAG'CTC
Cuts at: 3536 3849 4052 3536
Size: 313 203 6524
Fragments arranged by size:
6524 313 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 865 976 985 1018 1114 1495 1561 2271
Size: 738 111 9 33 96 381 66 710
Cuts at: 2271 2733 2976 3078 3199 3238 3522 4865 5297
Size: 462 243 102 121 39 284 1343 432
Cuts at: 5297 5650 6312 127
Size: 353 662 855
Fragments arranged by size:
1343 855 738 710 662 462 432 381
353 284 243 121 111 102 96 66
39 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 997 1081 1678 997
Size: 84 597 6359
Fragments arranged by size:
6359 597 84
EcoO109I rG'GnC_Cy
Cuts at: 596 1086 1920 1941 2366 2422 2509 2518 2709
Size: 490 834 21 425 56 87 9 191
Cuts at: 2709 3108 3241 4179 596
Size: 399 133 938 3457
Fragments arranged by size:
3457 938 834 490 425 399 191 133
87 56 21 9
EcoRI G'AATT_C
Cuts at: 4044 4044
Size: 7040
EcoRV GAT'ATC
Cuts at: 3197 3197
Size: 7040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2493 3006 5059 2493
Size: 513 2053 4474
Fragments arranged by size:
4474 2053 513
FspI TGC'GCA
Cuts at: 4821 4821
Size: 7040
HaeII r_GCGC'y
Cuts at: 135 713 724 1284 1422 1540 2657 3885 4474
Size: 578 11 560 138 118 1117 1228 589
Cuts at: 4474 4722 5952 6723 135
Size: 248 1230 771 452
Fragments arranged by size:
1230 1228 1117 771 589 578 560 452
248 138 118 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 1697 1729 2158 2190 3282 3300 3314
Size: 32 1318 32 429 32 1092 18 14
Cuts at: 3314 3332 4882 4912 4914 4944 347
Size: 18 1550 30 2 30 2443
Fragments arranged by size:
2443 1550 1318 1092 429 32 32 32
30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 4079 4079
Size: 7040
HindIII A'AGCT_T
Cuts at: 3607 3607
Size: 7040
KasI G'GCGC_C
Cuts at: 131 709 1418 2653 4470 4718 131
Size: 578 709 1235 1817 248 2453
Fragments arranged by size:
2453 1817 1235 709 578 248
KpnI G_GTAC'C
Cuts at: 4060 4060
Size: 7040
MluI A'CGCG_T
Cuts at: 6473 6473
Size: 7040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 579 2079 5799 5983 579
Size: 1500 3720 184 1636
Fragments arranged by size:
3720 1636 1500 184
MscI TGG'CCA
Cuts at: 787 1081 1613 1666 1905 3262 3569 4801 787
Size: 294 532 53 239 1357 307 1232 3026
Fragments arranged by size:
3026 1357 1232 532 307 294 239 53
MslI CAynn'nnrTG
Cuts at: 1556 1660 2575 3235 3967 5029 5156 1556
Size: 104 915 660 732 1062 127 3440
Fragments arranged by size:
3440 1062 915 732 660 127 104
MspA1I CmG'CkG
Cuts at: 137 188 1071 1491 1974 2566 2614 2753 2843
Size: 51 883 420 483 592 48 139 90
Cuts at: 2843 3559 3672 3756 3778 4151 4465 4825 5609
Size: 716 113 84 22 373 314 360 784
Cuts at: 5609 5854 137
Size: 245 1323
Fragments arranged by size:
1323 883 784 716 592 483 420 373
360 314 245 139 113 90 84 51
48 22
NaeI GCC'GGC
Cuts at: 2341 2688 5221 2341
Size: 347 2533 4160
Fragments arranged by size:
4160 2533 347
NarI GG'CG_CC
Cuts at: 132 710 1419 2654 4471 4719 132
Size: 578 709 1235 1817 248 2453
Fragments arranged by size:
2453 1817 1235 709 578 248
NcoI C'CATG_G
Cuts at: 107 1802 107
Size: 1695 5345
NgoMIV G'CCGG_C
Cuts at: 2339 2686 5219 2339
Size: 347 2533 4160
Fragments arranged by size:
4160 2533 347
NspI r_CATG'y
Cuts at: 1398 4093 4593 6196 1398
Size: 2695 500 1603 2242
Fragments arranged by size:
2695 2242 1603 500
PciI A'CATG_T
Cuts at: 6192 6192
Size: 7040
PflMI CCAn_nnn'nTGG
Cuts at: 996 996
Size: 7040
PfoI T'CCnGG_A
Cuts at: 2368 2368
Size: 7040
PmeI GTTT'AAAC
Cuts at: 4039 4039
Size: 7040
PmlI CAC'GTG
Cuts at: 630 630
Size: 7040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5486 5518 5486
Size: 32 7008
PpuMI rG'GwC_Cy
Cuts at: 596 1086 1941 2366 2422 3108 596
Size: 490 855 425 56 686 4528
Fragments arranged by size:
4528 855 686 490 425 56
PspOMI G'GGCC_C
Cuts at: 1485 2709 3240 4018 1485
Size: 1224 531 778 4507
Fragments arranged by size:
4507 1224 778 531
PspXI vC'TCGA_Gb
Cuts at: 2592 2592
Size: 7040
PstI C_TGCA'G
Cuts at: 683 860 2517 4087 4772 683
Size: 177 1657 1570 685 2951
Fragments arranged by size:
2951 1657 1570 685 177
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7040
PvuII CAG'CTG
Cuts at: 1071 2614 3559 3778 4465 4825 1071
Size: 1543 945 219 687 360 3286
Fragments arranged by size:
3286 1543 945 687 360 219
RsrII CG'GwC_CG
Cuts at: 5235 5235
Size: 7040
SacI G_AGCT'C
Cuts at: 3538 3851 4054 3538
Size: 313 203 6524
Fragments arranged by size:
6524 313 203
SacII CC_GC'GG
Cuts at: 138 3757 138
Size: 3619 3421
SalI G'TCGA_C
Cuts at: 4077 4077
Size: 7040
SanDI GG'GwC_CC
Cuts at: 2422 2422
Size: 7040
SapI GCTCTTCn'nnn_
Cuts at: 3175 5063 5273 3175
Size: 1888 210 4942
Fragments arranged by size:
4942 1888 210
SbfI CC_TGCA'GG
Cuts at: 2517 4087 2517
Size: 1570 5470
ScaI AGT'ACT
Cuts at: 6557 6557
Size: 7040
SexAI A'CCwGG_T
Cuts at: 1253 1951 1253
Size: 698 6342
SfcI C'TryA_G
Cuts at: 33 679 856 2229 2513 4083 4768 5736 5927
Size: 646 177 1373 284 1570 685 968 191
Cuts at: 5927 33
Size: 1146
Fragments arranged by size:
1570 1373 1146 968 685 646 284 191
177
SfiI GGCCn_nnn'nGGCC
Cuts at: 2516 2516
Size: 7040
SfoI GGC'GCC
Cuts at: 133 711 1420 2655 4472 4720 133
Size: 578 709 1235 1817 248 2453
Fragments arranged by size:
2453 1817 1235 709 578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7040
SmaI CCC'GGG
Cuts at: 436 581 3726 4062 436
Size: 145 3145 336 3414
Fragments arranged by size:
3414 3145 336 145
SmlI C'TyrA_G
Cuts at: 2592 4195 5110 5824 6086 2592
Size: 1603 915 714 262 3546
Fragments arranged by size:
3546 1603 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7040
SphI G_CATG'C
Cuts at: 4093 4593 4093
Size: 500 6540
SrfI GCCC'GGGC
Cuts at: 581 3726 581
Size: 3145 3895
StuI AGG'CCT
Cuts at: 2676 2676
Size: 7040
StyI C'CwwG_G
Cuts at: 107 281 650 1029 1082 1802 2671 2703 3263
Size: 174 369 379 53 720 869 32 560
Cuts at: 3263 4174 4528 107
Size: 911 354 2619
Fragments arranged by size:
2619 911 869 720 560 379 369 354
174 53 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 246 417 1864 2014 3010 3133 3454 3534 4623
Size: 171 1447 150 996 123 321 80 1089
Cuts at: 4623 4756 5215 5424 246
Size: 133 459 209 1862
Fragments arranged by size:
1862 1447 1089 996 459 321 209 171
150 133 123 80
TatI w'GTAC_w
Cuts at: 1055 1514 1742 6555 1055
Size: 459 228 4813 1540
Fragments arranged by size:
4813 1540 459 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 536 568 2274 2293 2306 2325 3433
Size: 32 29 32 1706 19 13 19 1108
Cuts at: 3433 3465 6557 6589 475
Size: 32 3092 32 926
Fragments arranged by size:
3092 1706 1108 926 32 32 32 32
29 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 4837 4837
Size: 7040
XbaI T'CTAG_A
Cuts at: 4071 4071
Size: 7040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1089 1793 3270 4535 663
Size: 426 704 1477 1265 3168
Fragments arranged by size:
3168 1477 1265 704 426
XhoI C'TCGA_G
Cuts at: 2592 2592
Size: 7040
XmaI C'CCGG_G
Cuts at: 434 579 3724 4060 434
Size: 145 3145 336 3414
Fragments arranged by size:
3414 3145 336 145
ZraI GAC'GTC
Cuts at: 3033 3162 3033
Size: 129 6911
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BfrBI BmtI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PsrI SgrAI SnaBI SspI SwaI
XmnI