Restriction map for
pF1KA0562
(Circular) (Six-base) MAPSORT of: pF1KA0562.seq Check: 8777 from: 1 to: 5888
pF1KA0562 5888 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1184 2926 1184
Size: 1742 4146
Acc65I G'GTAC_C
Cuts at: 2904 2904
Size: 5888
AclI AA'CG_TT
Cuts at: 5728 5728
Size: 5888
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 1114 1829 2270 2345 2646 3713 4145 4892
Size: 967 715 441 75 301 1067 432 747
Cuts at: 4892 5160 147
Size: 268 875
Fragments arranged by size:
1067 967 875 747 715 441 432 301
268 75
AflIII A'CryG_T
Cuts at: 398 2012 3240 4344 5321 398
Size: 1614 1228 1104 977 965
Fragments arranged by size:
1614 1228 1104 977 965
AgeI A'CCGG_T
Cuts at: 2636 4276 2636
Size: 1640 4248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 962 308
Size: 654 5234
AlwNI CAG_nnn'CTG
Cuts at: 2140 2320 3193 4760 2140
Size: 180 873 1567 3268
Fragments arranged by size:
3268 1567 873 180
ApaI G_GGCC'C
Cuts at: 2704 2704
Size: 5888
ApaLI G'TGCA_C
Cuts at: 4658 4658
Size: 5888
ApoI r'AATT_y
Cuts at: 1755 2240 2892 5241 5497 1755
Size: 485 652 2349 256 2146
Fragments arranged by size:
2349 2146 652 485 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5888
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5888
AvaI C'yCGr_G
Cuts at: 170 437 602 1914 2908 170
Size: 267 165 1312 994 3150
Fragments arranged by size:
3150 1312 994 267 165
BamHI G'GATC_C
Cuts at: 2913 2913
Size: 5888
BanI G'GyrC_C
Cuts at: 2904 3318 3566 3601 2904
Size: 414 248 35 5191
Fragments arranged by size:
5191 414 248 35
BanII G_rGCy'C
Cuts at: 179 1364 2704 2860 2902 3932 179
Size: 1185 1340 156 42 1030 2135
Fragments arranged by size:
2135 1340 1185 1030 156 42
BbeI G_GCGC'C
Cuts at: 3322 3570 3322
Size: 248 5640
BbsI GAAGACnn'nnnn_
Cuts at: 161 393 905 1011 161
Size: 232 512 106 5038
Fragments arranged by size:
5038 512 232 106
BbvCI CC'TCA_GC
Cuts at: 1169 1430 1169
Size: 261 5627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 631 665 2706 2740 90
Size: 34 507 34 2041 34 3238
Fragments arranged by size:
3238 2041 507 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3786 4547 5888 3786
Size: 761 1341 3786
Fragments arranged by size:
3786 1341 761
BclI T'GATC_A
Cuts at: 1625 3408 1625
Size: 1783 4105
BglI GCCn_nnn'nGGC
Cuts at: 2708 2708
Size: 5888
BglII A'GATC_T
Cuts at: 3403 5440 3403
Size: 2037 3851
BlpI GC'TnA_GC
Cuts at: 2778 3000 2778
Size: 222 5666
Bme1580I G_kGCm'C
Cuts at: 171 635 1906 1959 2154 2704 3513 3606 4662
Size: 464 1271 53 195 550 809 93 1056
Cuts at: 4662 171
Size: 1397
Fragments arranged by size:
1397 1271 1056 809 550 464 195 93
53
BmgBI CAC'GTC
Cuts at: 1448 1448
Size: 5888
BmrI ACTGGGnnnn_n'
Cuts at: 3100 3181 3516 5660 3100
Size: 81 335 2144 3328
Fragments arranged by size:
3328 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4255 4255
Size: 5888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1009 1739 1981 2039 2062 2690 2844 988
Size: 21 730 242 58 23 628 154 4032
Fragments arranged by size:
4032 730 628 242 154 58 23 21
Bpu10I CC'TnA_GC
Cuts at: 1169 1430 1169
Size: 261 5627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1401 3064 3943 4435 4733 1401
Size: 1663 879 492 298 2556
Fragments arranged by size:
2556 1663 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 557 5592 557
Size: 5035 853
BsaAI yAC'GTr
Cuts at: 399 3241 399
Size: 2842 3046
BsaBI GATnn'nnATC
Cuts at: 6 1211 3402 5439 6
Size: 1205 2191 2037 455
Fragments arranged by size:
2191 2037 1205 455
BsaHI Gr'CG_yC
Cuts at: 3319 3567 4269 5765 3319
Size: 248 702 1496 3442
Fragments arranged by size:
3442 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2619 2636 3077 3598 4276 4550 4697 5108 2619
Size: 17 441 521 678 274 147 411 3399
Fragments arranged by size:
3399 678 521 441 411 274 147 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1149 1179 1149
Size: 30 5858
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1157 1157
Size: 5888
BseYI C'CCAG_C
Cuts at: 2703 3313 4071 4648 5144 2703
Size: 610 758 577 496 3447
Fragments arranged by size:
3447 758 610 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1843 2697 958
Size: 885 854 4149
Fragments arranged by size:
4149 885 854
BsiEI CG_ry'CG
Cuts at: 104 3430 3476 4684 104
Size: 3326 46 1208 1308
Fragments arranged by size:
3326 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 2351 2501 2860 2902 3680 3870 4662 179
Size: 2172 150 359 42 778 190 792 1405
Fragments arranged by size:
2172 1405 792 778 359 190 150 42
BsiWI C'GTAC_G
Cuts at: 665 2870 665
Size: 2205 3683
BsmI GAATG_Cn'
Cuts at: 1173 1173
Size: 5888
BsmBI CGTCTCn'nnnn_
Cuts at: 1981 1981
Size: 5888
Bsp1286I G_dGCh'C
Cuts at: 171 179 635 1364 1906 1959 2154 2351 2501
Size: 8 456 729 542 53 195 197 150
Cuts at: 2501 2704 2860 2902 3513 3606 3680 3870 3932
Size: 203 156 42 611 93 74 190 62
Cuts at: 3932 4662 171
Size: 730 1397
Fragments arranged by size:
1397 730 729 611 542 456 203 197
195 190 156 150 93 74 62 53
42 8
BspEI T'CCGG_A
Cuts at: 5108 5108
Size: 5888
BspHI T'CATG_A
Cuts at: 179 2652 5064 179
Size: 2473 2412 1003
Fragments arranged by size:
2473 2412 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2732 2938 3152 3454 3835 2732
Size: 206 214 302 381 4785
Fragments arranged by size:
4785 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 378 1251 4180 4234 5718 5883 50
Size: 328 873 2929 54 1484 165 55
Fragments arranged by size:
2929 1484 873 328 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 1865 3800 824
Size: 1041 1935 2912
Fragments arranged by size:
2912 1935 1041
BsrFI r'CCGG_y
Cuts at: 2636 2707 3886 4067 4276 2636
Size: 71 1179 181 209 4248
Fragments arranged by size:
4248 1179 209 181 71
BsrGI T'GTAC_A
Cuts at: 2015 2015
Size: 5888
BssSI C'ACGA_G
Cuts at: 4159 4517 4159
Size: 358 5530
BstAPI GCAn_nnn'nTGC
Cuts at: 1052 1474 2320 3265 1052
Size: 422 846 945 3675
Fragments arranged by size:
3675 945 846 422
BstBI TT'CG_AA
Cuts at: 384 1998 4249 384
Size: 1614 2251 2023
Fragments arranged by size:
2251 2023 1614
BstEII G'GTnAC_C
Cuts at: 212 212
Size: 5888
BstXI CCAn_nnnn'nTGG
Cuts at: 121 628 121
Size: 507 5381
BstYI r'GATC_y
Cuts at: 1 726 1206 2415 2825 2913 3380 3403 3738
Size: 725 480 1209 410 88 467 23 335
Cuts at: 3738 3984 4996 5082 5094 5440 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1209 1012 725 480 467 449 410 346
335 246 88 86 23 12
BtgI C'CryG_G
Cuts at: 887 887
Size: 5888
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1340 3838 4019 4182 1340
Size: 2498 181 163 3046
Fragments arranged by size:
3046 2498 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1468 2460 2559 2758 3131 1295
Size: 173 992 99 199 373 4052
Fragments arranged by size:
4052 992 373 199 173 99
ClaI AT'CG_AT
Cuts at: 1403 1403
Size: 5888
DraI TTT'AAA
Cuts at: 1529 1782 2887 1529
Size: 253 1105 4530
Fragments arranged by size:
4530 1105 253
DraIII CAC_nnn'GTG
Cuts at: 1683 1683
Size: 5888
DrdI GACnn_nn'nnGTC
Cuts at: 3594 4452 3594
Size: 858 5030
EaeI y'GGCC_r
Cuts at: 2786 3473 3647 4038 4065 5410 2786
Size: 687 174 391 27 1345 3264
Fragments arranged by size:
3264 1345 687 391 174 27
EagI C'GGCC_G
Cuts at: 3473 3473
Size: 5888
EarI CTCTTCn'nnn_
Cuts at: 1262 1560 1848 2567 2669 3911 4121 1262
Size: 298 288 719 102 1242 210 3029
Fragments arranged by size:
3029 1242 719 298 288 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2858 4096 4406 4552 5698 2858
Size: 1238 310 146 1146 3048
Fragments arranged by size:
3048 1238 1146 310 146
EcoICRI GAG'CTC
Cuts at: 177 2858 2900 177
Size: 2681 42 3165
Fragments arranged by size:
3165 2681 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 988 1009 1114 1739 1829 1981 2039 2062
Size: 841 21 105 625 90 152 58 23
Cuts at: 2062 2270 2345 2646 2690 2844 3713 4145 4892
Size: 208 75 301 44 154 869 432 747
Cuts at: 4892 5160 147
Size: 268 875
Fragments arranged by size:
875 869 841 747 625 432 301 268
208 154 152 105 90 75 58 44
23 21
EcoO109I rG'GnC_Cy
Cuts at: 645 2751 2797 3027 645
Size: 2106 46 230 3506
Fragments arranged by size:
3506 2106 230 46
EcoRI G'AATT_C
Cuts at: 2892 2892
Size: 5888
EcoRV GAT'ATC
Cuts at: 2483 2483
Size: 5888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3907 3907
Size: 5888
FspI TGC'GCA
Cuts at: 2728 3669 2728
Size: 941 4947
HaeII r_GCGC'y
Cuts at: 855 3322 3570 4592 5571 855
Size: 2467 248 1022 979 1172
Fragments arranged by size:
2467 1172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 673 705 1535 1567 2472 2504 3730
Size: 32 525 32 830 32 905 32 1226
Cuts at: 3730 3760 3762 3792 116
Size: 30 2 30 2212
Fragments arranged by size:
2212 1226 905 830 525 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 510 2927 510
Size: 2417 3471
HindIII A'AGCT_T
Cuts at: 342 2207 342
Size: 1865 4023
KasI G'GCGC_C
Cuts at: 3318 3566 3318
Size: 248 5640
KpnI G_GTAC'C
Cuts at: 2908 2908
Size: 5888
MluI A'CGCG_T
Cuts at: 5321 5321
Size: 5888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1488 4559 4743 1488
Size: 3071 184 2633
Fragments arranged by size:
3071 2633 184
MscI TGG'CCA
Cuts at: 3649 3649
Size: 5888
MslI CAynn'nnrTG
Cuts at: 1405 3877 4004 1405
Size: 2472 127 3289
Fragments arranged by size:
3289 2472 127
MspA1I CmG'CkG
Cuts at: 966 1750 2223 2782 2999 3313 3673 4686 4931
Size: 784 473 559 217 314 360 1013 245
Cuts at: 4931 966
Size: 1923
Fragments arranged by size:
1923 1013 784 559 473 360 314 245
217
NaeI GCC'GGC
Cuts at: 2709 4069 2709
Size: 1360 4528
NarI GG'CG_CC
Cuts at: 3319 3567 3319
Size: 248 5640
NgoMIV G'CCGG_C
Cuts at: 2707 4067 2707
Size: 1360 4528
NspI r_CATG'y
Cuts at: 1347 2016 2941 3441 4348 1347
Size: 669 925 500 907 2887
Fragments arranged by size:
2887 925 907 669 500
PciI A'CATG_T
Cuts at: 2012 4344 2012
Size: 2332 3556
PfoI T'CCnGG_A
Cuts at: 983 2039 2339 983
Size: 1056 300 4532
Fragments arranged by size:
4532 1056 300
PmeI GTTT'AAAC
Cuts at: 2887 2887
Size: 5888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 5027
Size: 32 5856
PpuMI rG'GwC_Cy
Cuts at: 645 645
Size: 5888
PspOMI G'GGCC_C
Cuts at: 2700 2700
Size: 5888
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2071 2103 2071
Size: 32 5856
PstI C_TGCA'G
Cuts at: 287 573 1182 2747 2765 2935 3620 287
Size: 286 609 1565 18 170 685 2555
Fragments arranged by size:
2555 1565 685 609 286 170 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5888
PvuII CAG'CTG
Cuts at: 966 1750 2223 2782 3313 3673 966
Size: 784 473 559 531 360 3181
Fragments arranged by size:
3181 784 559 531 473 360
RsrII CG'GwC_CG
Cuts at: 4083 4083
Size: 5888
SacI G_AGCT'C
Cuts at: 179 2860 2902 179
Size: 2681 42 3165
Fragments arranged by size:
3165 2681 42
SalI G'TCGA_C
Cuts at: 2925 2925
Size: 5888
SapI GCTCTTCn'nnn_
Cuts at: 1560 2567 3911 4121 1560
Size: 1007 1344 210 3327
Fragments arranged by size:
3327 1344 1007 210
SbfI CC_TGCA'GG
Cuts at: 2935 2935
Size: 5888
ScaI AGT'ACT
Cuts at: 5405 5405
Size: 5888
SexAI A'CCwGG_T
Cuts at: 1817 1817
Size: 5888
SfcI C'TryA_G
Cuts at: 33 283 569 1178 2132 2168 2743 2761 2931
Size: 250 286 609 954 36 575 18 170
Cuts at: 2931 3616 4609 4800 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 609 575 286 250
191 170 36 18
SfoI GGC'GCC
Cuts at: 3320 3568 3320
Size: 248 5640
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5888
SmaI CCC'GGG
Cuts at: 172 439 2910 172
Size: 267 2471 3150
Fragments arranged by size:
3150 2471 267
SmlI C'TyrA_G
Cuts at: 602 1380 3043 3958 4450 4712 602
Size: 778 1663 915 492 262 1778
Fragments arranged by size:
1778 1663 915 778 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5888
SphI G_CATG'C
Cuts at: 2941 3441 2941
Size: 500 5388
SspI AAT'ATT
Cuts at: 355 355
Size: 5888
StuI AGG'CCT
Cuts at: 1378 1435 1378
Size: 57 5831
StyI C'CwwG_G
Cuts at: 627 855 914 1421 2801 3022 3376 627
Size: 228 59 507 1380 221 354 3139
Fragments arranged by size:
3139 1380 507 354 228 221 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 3471 3604 4063 4272 194
Size: 3277 133 459 209 1810
Fragments arranged by size:
3277 1810 459 209 133
TatI w'GTAC_w
Cuts at: 2015 5403 2015
Size: 3388 2500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2017 2049 5405 5437 2017
Size: 32 3356 32 2468
Fragments arranged by size:
3356 2468 32 32
Tth111I GACn'n_nGTC
Cuts at: 1449 3685 1449
Size: 2236 3652
XbaI T'CTAG_A
Cuts at: 654 2355 2919 654
Size: 1701 564 3623
Fragments arranged by size:
3623 1701 564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1407 3383 1407
Size: 1976 3912
XhoI C'TCGA_G
Cuts at: 602 602
Size: 5888
XmaI C'CCGG_G
Cuts at: 170 437 2908 170
Size: 267 2471 3150
Fragments arranged by size:
3150 2471 267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BfrBI BmtI BssHII BstZ17I Bsu36I CspCI
EcoNI FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI