Restriction map for
pF1KA0567
(Circular) (Six-base) MAPSORT of: pF1KA0567.seq Check: 1201 from: 1 to: 5993
pF1KA0567 5993 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1514 2754 3031 1514
Size: 1240 277 4476
Fragments arranged by size:
4476 1240 277
Acc65I G'GTAC_C
Cuts at: 3009 3009
Size: 5993
AclI AA'CG_TT
Cuts at: 5833 5833
Size: 5993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 935 2831 2882 3818 4250 4997 5265 745
Size: 190 1896 51 936 432 747 268 1473
Fragments arranged by size:
1896 1473 936 747 432 268 190 51
AflII C'TTAA_G
Cuts at: 886 886
Size: 5993
AflIII A'CryG_T
Cuts at: 905 1075 1816 2901 3345 4449 5426 905
Size: 170 741 1085 444 1104 977 1472
Fragments arranged by size:
1472 1104 1085 977 741 444 170
AgeI A'CCGG_T
Cuts at: 4381 4381
Size: 5993
AleI CACnn'nnGTG
Cuts at: 2062 2062
Size: 5993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2348 2348
Size: 5993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2557 2589 2557
Size: 32 5961
AlwNI CAG_nnn'CTG
Cuts at: 3298 4865 3298
Size: 1567 4426
ApaLI G'TGCA_C
Cuts at: 4763 4763
Size: 5993
ApoI r'AATT_y
Cuts at: 328 366 1680 1734 2040 2146 2939 2997 5346
Size: 38 1314 54 306 106 793 58 2349
Cuts at: 5346 5602 328
Size: 256 719
Fragments arranged by size:
2349 1314 793 719 306 256 106 58
54 38
AseI AT'TA_AT
Cuts at: 20 1316 20
Size: 1296 4697
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
AvaI C'yCGr_G
Cuts at: 1142 2576 3013 1142
Size: 1434 437 4122
Fragments arranged by size:
4122 1434 437
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1765 1798 1765
Size: 33 5960
BamHI G'GATC_C
Cuts at: 3018 3018
Size: 5993
BanI G'GyrC_C
Cuts at: 3009 3423 3671 3706 3009
Size: 414 248 35 5296
Fragments arranged by size:
5296 414 248 35
BanII G_rGCy'C
Cuts at: 3007 4037 3007
Size: 1030 4963
BbeI G_GCGC'C
Cuts at: 3427 3675 3427
Size: 248 5745
BbsI GAAGACnn'nnnn_
Cuts at: 593 2306 2640 2924 593
Size: 1713 334 284 3662
Fragments arranged by size:
3662 1713 334 284
BciVI GTATCCnnnnn_n'
Cuts at: 2373 2604 3891 4652 5993 2373
Size: 231 1287 761 1341 2373
Fragments arranged by size:
2373 1341 1287 761 231
BclI T'GATC_A
Cuts at: 465 3513 465
Size: 3048 2945
BglI GCCn_nnn'nGGC
Cuts at: 133 133
Size: 5993
BglII A'GATC_T
Cuts at: 1170 3508 5545 1170
Size: 2338 2037 1618
Fragments arranged by size:
2338 2037 1618
BlpI GC'TnA_GC
Cuts at: 3105 3105
Size: 5993
Bme1580I G_kGCm'C
Cuts at: 3618 3711 4767 3618
Size: 93 1056 4844
Fragments arranged by size:
4844 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3205 3286 3621 5765 3205
Size: 81 335 2144 3433
Fragments arranged by size:
3433 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4360 4360
Size: 5993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1067
Size: 5993
Bpu10I CC'TnA_GC
Cuts at: 634 634
Size: 5993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 2359 2908 3169 4048 4540 4838 2292
Size: 67 549 261 879 492 298 3447
Fragments arranged by size:
3447 879 549 492 298 261 67
BsaI GGTCTCn'nnnn_
Cuts at: 1240 2125 5697 1240
Size: 885 3572 1536
Fragments arranged by size:
3572 1536 885
BsaAI yAC'GTr
Cuts at: 319 3346 319
Size: 3027 2966
BsaBI GATnn'nnATC
Cuts at: 6 2310 3507 5544 6
Size: 2304 1197 2037 455
Fragments arranged by size:
2304 2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 3424 3672 4374 5870 3424
Size: 248 702 1496 3547
Fragments arranged by size:
3547 1496 702 248
BsaWI w'CCGG_w
Cuts at: 486 669 1874 2558 3182 3703 4381 4655 4802
Size: 183 1205 684 624 521 678 274 147
Cuts at: 4802 5213 486
Size: 411 1266
Fragments arranged by size:
1266 1205 684 678 624 521 411 274
183 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1065 1095 2485 2515 2573 2603 1065
Size: 30 1390 30 58 30 4455
Fragments arranged by size:
4455 1390 58 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2530 2594 2530
Size: 64 5929
BseYI C'CCAG_C
Cuts at: 2523 3418 4176 4753 5249 2523
Size: 895 758 577 496 3267
Fragments arranged by size:
3267 895 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 5993
BsiEI CG_ry'CG
Cuts at: 104 3535 3581 4789 104
Size: 3431 46 1208 1308
Fragments arranged by size:
3431 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2522 3007 3785 3975 4767 2522
Size: 485 778 190 792 3748
Fragments arranged by size:
3748 792 778 485 190
BsmI GAATG_Cn'
Cuts at: 869 2731 869
Size: 1862 4131
BsmBI CGTCTCn'nnnn_
Cuts at: 2377 2377
Size: 5993
Bsp1286I G_dGCh'C
Cuts at: 2522 3007 3618 3711 3785 3975 4037 4767 2522
Size: 485 611 93 74 190 62 730 3748
Fragments arranged by size:
3748 730 611 485 190 93 74 62
BspEI T'CCGG_A
Cuts at: 669 2558 5213 669
Size: 1889 2655 1449
Fragments arranged by size:
2655 1889 1449
BspHI T'CATG_A
Cuts at: 5169 5169
Size: 5993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3043 3257 3559 3940 3043
Size: 214 302 381 5096
Fragments arranged by size:
5096 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4285 4339 5823 5988 50
Size: 4235 54 1484 165 55
Fragments arranged by size:
4235 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2332 2736 3905 2332
Size: 404 1169 4420
Fragments arranged by size:
4420 1169 404
BsrFI r'CCGG_y
Cuts at: 3991 4172 4381 3991
Size: 181 209 5603
Fragments arranged by size:
5603 209 181
BssSI C'ACGA_G
Cuts at: 4264 4622 4264
Size: 358 5635
BstAPI GCAn_nnn'nTGC
Cuts at: 3370 3370
Size: 5993
BstBI TT'CG_AA
Cuts at: 1203 4354 1203
Size: 3151 2842
BstXI CCAn_nnnn'nTGG
Cuts at: 1952 1952
Size: 5993
BstYI r'GATC_y
Cuts at: 1 703 1057 1170 1423 2592 3018 3485 3508
Size: 702 354 113 253 1169 426 467 23
Cuts at: 3508 3843 4089 5101 5187 5199 5545 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1169 1012 702 467 449 426 354 346
335 253 246 113 86 23 12
BstZ17I GTA'TAC
Cuts at: 2755 2755
Size: 5993
Bsu36I CC'TnA_GG
Cuts at: 660 660
Size: 5993
BtgI C'CryG_G
Cuts at: 973 973
Size: 5993
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3943 4124 4287 3943
Size: 181 163 5649
Fragments arranged by size:
5649 181 163
BtsI GCAGTG_nn'
Cuts at: 3236 3236
Size: 5993
ClaI AT'CG_AT
Cuts at: 548 548
Size: 5993
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1960 1995 1960
Size: 35 5958
DraI TTT'AAA
Cuts at: 1131 2645 2992 1131
Size: 1514 347 4132
Fragments arranged by size:
4132 1514 347
DraIII CAC_nnn'GTG
Cuts at: 1119 1722 1119
Size: 603 5390
DrdI GACnn_nn'nnGTC
Cuts at: 3699 4557 3699
Size: 858 5135
EaeI y'GGCC_r
Cuts at: 373 3578 3752 4143 4170 5515 373
Size: 3205 174 391 27 1345 851
Fragments arranged by size:
3205 1345 851 391 174 27
EagI C'GGCC_G
Cuts at: 3578 3578
Size: 5993
EarI CTCTTCn'nnn_
Cuts at: 300 1582 1661 2348 2673 2978 4016 4226 300
Size: 1282 79 687 325 305 1038 210 2067
Fragments arranged by size:
2067 1282 1038 687 325 305 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 4201 4511 4657 5803 2929
Size: 1272 310 146 1146 3119
Fragments arranged by size:
3119 1272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3005 3005
Size: 5993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 935 1067 2831 2882 3818 4250 4997 5265
Size: 190 132 1764 51 936 432 747 268
Cuts at: 5265 745
Size: 1473
Fragments arranged by size:
1764 1473 936 747 432 268 190 132
51
EcoO109I rG'GnC_Cy
Cuts at: 1108 1270 3132 1108
Size: 162 1862 3969
Fragments arranged by size:
3969 1862 162
EcoRI G'AATT_C
Cuts at: 1680 2040 2997 1680
Size: 360 957 4676
Fragments arranged by size:
4676 957 360
EcoRV GAT'ATC
Cuts at: 2070 2070
Size: 5993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1189 2968 4012 1189
Size: 1779 1044 3170
Fragments arranged by size:
3170 1779 1044
FspI TGC'GCA
Cuts at: 2030 3774 2030
Size: 1744 4249
HaeII r_GCGC'y
Cuts at: 3427 3675 4697 5676 3427
Size: 248 1022 979 3744
Fragments arranged by size:
3744 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 450 482 688 720 916 948 1065 1092 1097
Size: 32 206 32 196 32 117 27 5
Cuts at: 1097 1124 1237 1269 3835 3865 3867 3897 450
Size: 27 113 32 2566 30 2 30 2546
Fragments arranged by size:
2566 2546 206 196 117 113 32 32
32 32 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 1299 3032 1299
Size: 1733 4260
HindIII A'AGCT_T
Cuts at: 253 883 1376 1589 2074 2598 253
Size: 630 493 213 485 524 3648
Fragments arranged by size:
3648 630 524 493 485 213
KasI G'GCGC_C
Cuts at: 3423 3671 3423
Size: 248 5745
KpnI G_GTAC'C
Cuts at: 3013 3013
Size: 5993
MfeI C'AATT_G
Cuts at: 2393 2393
Size: 5993
MluI A'CGCG_T
Cuts at: 2901 5426 2901
Size: 2525 3468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 969 1001 2702 4664 4848 969
Size: 32 1701 1962 184 2114
Fragments arranged by size:
2114 1962 1701 184 32
MscI TGG'CCA
Cuts at: 375 3754 375
Size: 3379 2614
MslI CAynn'nnrTG
Cuts at: 2062 3982 4109 2062
Size: 1920 127 3946
Fragments arranged by size:
3946 1920 127
MspA1I CmG'CkG
Cuts at: 130 2026 3104 3418 3778 4791 5036 130
Size: 1896 1078 314 360 1013 245 1087
Fragments arranged by size:
1896 1087 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4174 4174
Size: 5993
NarI GG'CG_CC
Cuts at: 3424 3672 3424
Size: 248 5745
NgoMIV G'CCGG_C
Cuts at: 4172 4172
Size: 5993
NspI r_CATG'y
Cuts at: 909 1386 1707 2766 3046 3546 4453 909
Size: 477 321 1059 280 500 907 2449
Fragments arranged by size:
2449 1059 907 500 477 321 280
PciI A'CATG_T
Cuts at: 905 4449 905
Size: 3544 2449
PmeI GTTT'AAAC
Cuts at: 2992 2992
Size: 5993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5132 5164 5132
Size: 32 5961
PpuMI rG'GwC_Cy
Cuts at: 1108 1108
Size: 5993
PsiI TTA'TAA
Cuts at: 953 953
Size: 5993
PstI C_TGCA'G
Cuts at: 2366 3040 3725 2366
Size: 674 685 4634
Fragments arranged by size:
4634 685 674
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5993
PvuII CAG'CTG
Cuts at: 2026 3418 3778 2026
Size: 1392 360 4241
Fragments arranged by size:
4241 1392 360
RsrII CG'GwC_CG
Cuts at: 4188 4188
Size: 5993
SacI G_AGCT'C
Cuts at: 3007 3007
Size: 5993
SalI G'TCGA_C
Cuts at: 3030 3030
Size: 5993
SapI GCTCTTCn'nnn_
Cuts at: 1582 2978 4016 4226 1582
Size: 1396 1038 210 3349
Fragments arranged by size:
3349 1396 1038 210
SbfI CC_TGCA'GG
Cuts at: 3040 3040
Size: 5993
ScaI AGT'ACT
Cuts at: 5510 5510
Size: 5993
SexAI A'CCwGG_T
Cuts at: 1305 1305
Size: 5993
SfcI C'TryA_G
Cuts at: 33 1279 2362 3036 3721 4714 4905 33
Size: 1246 1083 674 685 993 191 1121
Fragments arranged by size:
1246 1121 1083 993 685 674 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 133 133
Size: 5993
SfoI GGC'GCC
Cuts at: 3425 3673 3425
Size: 248 5745
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
SmaI CCC'GGG
Cuts at: 3015 3015
Size: 5993
SmlI C'TyrA_G
Cuts at: 886 2271 2374 2923 3148 4063 4555 4817 886
Size: 1385 103 549 225 915 492 262 2062
Fragments arranged by size:
2062 1385 915 549 492 262 225 103
SnaBI TAC'GTA
Cuts at: 319 319
Size: 5993
SpeI A'CTAG_T
Cuts at: 67 1012 67
Size: 945 5048
SphI G_CATG'C
Cuts at: 1707 2766 3046 3546 1707
Size: 1059 280 500 4154
Fragments arranged by size:
4154 1059 500 280
SspI AAT'ATT
Cuts at: 1046 1046
Size: 5993
StyI C'CwwG_G
Cuts at: 3127 3481 3127
Size: 354 5639
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2318 2537 3576 3709 4168 4377 2318
Size: 219 1039 133 459 209 3934
Fragments arranged by size:
3934 1039 459 219 209 133
TatI w'GTAC_w
Cuts at: 5508 5508
Size: 5993
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5510 5542 5510
Size: 32 5961
Tth111I GACn'n_nGTC
Cuts at: 119 1459 3790 119
Size: 1340 2331 2322
Fragments arranged by size:
2331 2322 1340
XbaI T'CTAG_A
Cuts at: 3024 3024
Size: 5993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1515 2487 3488 1515
Size: 972 1001 4020
Fragments arranged by size:
4020 1001 972
XmaI C'CCGG_G
Cuts at: 3013 3013
Size: 5993
XmnI GAAnn'nnTTC
Cuts at: 770 1964 2972 770
Size: 1194 1008 3791
Fragments arranged by size:
3791 1194 1008
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI AvrII BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
EcoNI FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SgrAI SrfI StuI
SwaI XhoI ZraI