Restriction map for
pF1KA0571
(Circular) (Six-base) MAPSORT of: pF1KA0571.seq Check: 9335 from: 1 to: 5027
pF1KA0571 5027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1387 1387
Size: 5027
AccI GT'mk_AC
Cuts at: 926 1418 2065 926
Size: 492 647 3888
Fragments arranged by size:
3888 647 492
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 2852 3284 3637 4299 1358
Size: 1494 432 353 662 2086
Fragments arranged by size:
2086 1494 662 432 353
AflIII A'CryG_T
Cuts at: 2379 4179 4460 2379
Size: 1800 281 2946
Fragments arranged by size:
2946 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 703 703
Size: 5027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 218 218
Size: 5027
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 136
Size: 32 4995
AlwNI CAG_nnn'CTG
Cuts at: 1768 1953 2332 3770 1768
Size: 185 379 1438 3025
Fragments arranged by size:
3025 1438 379 185
ApaI G_GGCC'C
Cuts at: 1443 1443
Size: 5027
ApaLI G'TGCA_C
Cuts at: 1486 3865 1486
Size: 2379 2648
ApoI r'AATT_y
Cuts at: 772 2031 4380 4636 772
Size: 1259 2349 256 1163
Fragments arranged by size:
2349 1259 1163 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 2047 2047
Size: 5027
AvrII C'CTAG_G
Cuts at: 973 973
Size: 5027
BamHI G'GATC_C
Cuts at: 2052 2052
Size: 5027
BanI G'GyrC_C
Cuts at: 634 2011 2043 2457 2705 2740 634
Size: 1377 32 414 248 35 2921
Fragments arranged by size:
2921 1377 414 248 35 32
BanII G_rGCy'C
Cuts at: 428 1435 1443 1861 2041 3071 428
Size: 1007 8 418 180 1030 2384
Fragments arranged by size:
2384 1030 1007 418 180 8
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 1346 1948 1346
Size: 602 4425
BbvCI CC'TCA_GC
Cuts at: 356 390 468 1100 356
Size: 34 78 632 4283
Fragments arranged by size:
4283 632 78 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 891 925 1119 1153 891
Size: 34 194 34 4765
Fragments arranged by size:
4765 194 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2925 3981 5027 2925
Size: 1056 1046 2925
Fragments arranged by size:
2925 1056 1046
BclI T'GATC_A
Cuts at: 2547 2547
Size: 5027
BglI GCCn_nnn'nGGC
Cuts at: 759 842 759
Size: 83 4944
BglII A'GATC_T
Cuts at: 335 1132 2542 4579 335
Size: 797 1410 2037 783
Fragments arranged by size:
2037 1410 797 783
BlpI GC'TnA_GC
Cuts at: 422 427 2139 422
Size: 5 1712 3310
Fragments arranged by size:
3310 1712 5
Bme1580I G_kGCm'C
Cuts at: 637 1355 1443 1490 2652 2745 3869 637
Size: 718 88 47 1162 93 1124 1795
Fragments arranged by size:
1795 1162 1124 718 93 88 47
BmgBI CAC'GTC
Cuts at: 1983 1983
Size: 5027
BmrI ACTGGGnnnn_n'
Cuts at: 1671 1756 1778 2239 2320 2655 4799 1671
Size: 85 22 461 81 335 2144 1899
Fragments arranged by size:
2144 1899 461 335 85 81 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 5027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 468 1650 401
Size: 67 1182 3778
Fragments arranged by size:
3778 1182 67
Bpu10I CC'TnA_GC
Cuts at: 356 390 468 1100 356
Size: 34 78 632 4283
Fragments arranged by size:
4283 632 78 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 3082 3796 4094 2203
Size: 879 714 298 3136
Fragments arranged by size:
3136 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1101 1207 4731 1101
Size: 106 3524 1397
Fragments arranged by size:
3524 1397 106
BsaAI yAC'GTr
Cuts at: 2380 2380
Size: 5027
BsaBI GATnn'nnATC
Cuts at: 6 2541 4578 6
Size: 2535 2037 455
Fragments arranged by size:
2535 2037 455
BsaHI Gr'CG_yC
Cuts at: 883 2458 2706 3408 4904 883
Size: 1575 248 702 1496 1006
Fragments arranged by size:
1575 1496 1006 702 248
BsaWI w'CCGG_w
Cuts at: 2216 2737 3415 3826 3973 4247 2216
Size: 521 678 411 147 274 2996
Fragments arranged by size:
2996 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 1654 1684 136
Size: 30 1488 30 3479
Fragments arranged by size:
3479 1488 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 672 1662 373
Size: 299 990 3738
Fragments arranged by size:
3738 990 299
BseYI C'CCAG_C
Cuts at: 886 1498 2452 3210 3875 4283 886
Size: 612 954 758 665 408 1630
Fragments arranged by size:
1630 954 758 665 612 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1268 921
Size: 347 4680
BsiEI CG_ry'CG
Cuts at: 104 1321 2569 2615 3845 104
Size: 1217 1248 46 1230 1286
Fragments arranged by size:
1286 1248 1230 1217 46
BsiHKAI G_wGCw'C
Cuts at: 367 428 1490 1819 1861 2041 2819 3009 3869
Size: 61 1062 329 42 180 778 190 860
Cuts at: 3869 367
Size: 1525
Fragments arranged by size:
1525 1062 860 778 329 190 180 61
42
Bsp1286I G_dGCh'C
Cuts at: 367 428 637 1355 1435 1443 1490 1819 1861
Size: 61 209 718 80 8 47 329 42
Cuts at: 1861 2041 2652 2745 2819 3009 3071 3869 367
Size: 180 611 93 74 190 62 798 1525
Fragments arranged by size:
1525 798 718 611 329 209 190 180
93 80 74 62 61 47 42 8
BspHI T'CATG_A
Cuts at: 653 3459 653
Size: 2806 2221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 1387 2077 2291 2593 2974 186
Size: 1201 690 214 302 381 2239
Fragments arranged by size:
2239 1201 690 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 410 3319 3373 4857 5022 50
Size: 63 297 2909 54 1484 165 55
Fragments arranged by size:
2909 1484 297 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 699 1181 2939 699
Size: 482 1758 2787
Fragments arranged by size:
2787 1758 482
BsrFI r'CCGG_y
Cuts at: 394 758 1253 1819 3025 3206 394
Size: 364 495 566 1206 181 2215
Fragments arranged by size:
2215 1206 566 495 364 181
BsrGI T'GTAC_A
Cuts at: 672 869 672
Size: 197 4830
BssSI C'ACGA_G
Cuts at: 3298 4006 3298
Size: 708 4319
BstAPI GCAn_nnn'nTGC
Cuts at: 607 616 2404 607
Size: 9 1788 3230
Fragments arranged by size:
3230 1788 9
BstBI TT'CG_AA
Cuts at: 3388 3388
Size: 5027
BstEII G'GTnAC_C
Cuts at: 115 115
Size: 5027
BstXI CCAn_nnnn'nTGG
Cuts at: 340 694 1014 1380 340
Size: 354 320 366 3987
Fragments arranged by size:
3987 366 354 320
BstYI r'GATC_y
Cuts at: 1 335 968 1132 2052 2519 2542 2877 3123
Size: 334 633 164 920 467 23 335 246
Cuts at: 3123 3429 3441 3527 4579 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 920 633 467 449 335 334 306
246 164 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 560 1582 560
Size: 1022 4005
BtgI C'CryG_G
Cuts at: 729 816 1385 729
Size: 87 569 4371
Fragments arranged by size:
4371 569 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 2270 2270
Size: 5027
ClaI AT'CG_AT
Cuts at: 1601 1601
Size: 5027
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 899 1234 899
Size: 335 4692
DrdI GACnn_nn'nnGTC
Cuts at: 2733 4077 2733
Size: 1344 3683
EaeI y'GGCC_r
Cuts at: 819 944 1016 1382 2612 2786 3177 3204 4549
Size: 125 72 366 1230 174 391 27 1345
Cuts at: 4549 819
Size: 1297
Fragments arranged by size:
1345 1297 1230 391 366 174 125 72
27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 293 417 591 1730 1807 3050 3260 293
Size: 124 174 1139 77 1243 210 2060
Fragments arranged by size:
2060 1243 1139 210 174 124 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3977 4123 4837 3235
Size: 742 146 714 3425
Fragments arranged by size:
3425 742 714 146
EcoICRI GAG'CTC
Cuts at: 426 1859 2039 426
Size: 1433 180 3414
Fragments arranged by size:
3414 1433 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 468 1358 1650 2852 3284 3637 4299 401
Size: 67 890 292 1202 432 353 662 1129
Fragments arranged by size:
1202 1129 890 662 432 353 292 67
EcoO109I rG'GnC_Cy
Cuts at: 915 1439 1495 1950 2166 915
Size: 524 56 455 216 3776
Fragments arranged by size:
3776 524 455 216 56
EcoRI G'AATT_C
Cuts at: 772 2031 772
Size: 1259 3768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 5027
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 2461 2709 3939 4710 2461
Size: 248 1230 771 2778
Fragments arranged by size:
2778 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 689 705 721 737 969 1001 1100
Size: 32 209 16 16 16 232 32 99
Cuts at: 1100 1132 2869 2899 2901 2931 448
Size: 32 1737 30 2 30 2544
Fragments arranged by size:
2544 1737 232 209 99 32 32 32
30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 708 1533 2066 708
Size: 825 533 3669
Fragments arranged by size:
3669 825 533
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3786 3970 3786
Size: 184 4843
MscI TGG'CCA
Cuts at: 821 1018 1384 2788 821
Size: 197 366 1404 3060
Fragments arranged by size:
3060 1404 366 197
MslI CAynn'nnrTG
Cuts at: 1012 1086 1426 3016 3143 1012
Size: 74 340 1590 127 2896
Fragments arranged by size:
2896 1590 340 127 74
MspA1I CmG'CkG
Cuts at: 421 670 2138 2452 2812 3596 3841 421
Size: 249 1468 314 360 784 245 1607
Fragments arranged by size:
1607 1468 784 360 314 249 245
NaeI GCC'GGC
Cuts at: 760 3208 760
Size: 2448 2579
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 729 816 1385 729
Size: 87 569 4371
Fragments arranged by size:
4371 569 87
NgoMIV G'CCGG_C
Cuts at: 758 3206 758
Size: 2448 2579
NspI r_CATG'y
Cuts at: 534 2080 2580 4183 534
Size: 1546 500 1603 1378
Fragments arranged by size:
1603 1546 1378 500
PasI CC'CwG_GG
Cuts at: 687 1435 687
Size: 748 4279
PciI A'CATG_T
Cuts at: 4179 4179
Size: 5027
PflMI CCAn_nnn'nTGG
Cuts at: 1234 1637 1234
Size: 403 4624
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 136 168 3473 3505 136
Size: 32 3305 32 1658
Fragments arranged by size:
3305 1658 32 32
PpuMI rG'GwC_Cy
Cuts at: 915 915
Size: 5027
PspOMI G'GGCC_C
Cuts at: 1439 1439
Size: 5027
PstI C_TGCA'G
Cuts at: 236 1958 2074 2759 236
Size: 1722 116 685 2504
Fragments arranged by size:
2504 1722 685 116
PvuI CG_AT'CG
Cuts at: 104 1321 104
Size: 1217 3810
PvuII CAG'CTG
Cuts at: 421 670 2452 2812 421
Size: 249 1782 360 2636
Fragments arranged by size:
2636 1782 360 249
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 428 1861 2041 428
Size: 1433 180 3414
Fragments arranged by size:
3414 1433 180
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 417 591 1807 3050 3260 417
Size: 174 1216 1243 210 2184
Fragments arranged by size:
2184 1243 1216 210 174
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 1681 4544 1681
Size: 2863 2164
SexAI A'CCwGG_T
Cuts at: 281 281
Size: 5027
SfcI C'TryA_G
Cuts at: 33 232 738 1954 2070 2755 3723 3914 33
Size: 199 506 1216 116 685 968 191 1146
Fragments arranged by size:
1216 1146 968 685 506 199 191 116
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 2049 2049
Size: 5027
SmlI C'TyrA_G
Cuts at: 2182 3097 3811 4073 2182
Size: 915 714 262 3136
Fragments arranged by size:
3136 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 2080 2580 2080
Size: 500 4527
StuI AGG'CCT
Cuts at: 209 209
Size: 5027
StyI C'CwwG_G
Cuts at: 729 816 827 973 1385 2006 2161 2515 729
Size: 87 11 146 412 621 155 354 3241
Fragments arranged by size:
3241 621 412 354 155 146 87 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1714 2610 2743 3202 3411 1714
Size: 896 133 459 209 3330
Fragments arranged by size:
3330 896 459 209 133
TatI w'GTAC_w
Cuts at: 672 869 1679 4542 672
Size: 197 810 2863 1157
Fragments arranged by size:
2863 1157 810 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1782 1814 4544 4576 1782
Size: 32 2730 32 2233
Fragments arranged by size:
2730 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 2824 2824
Size: 5027
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 2522
Size: 5027
XmaI C'CCGG_G
Cuts at: 2047 2047
Size: 5027
XmnI GAAnn'nnTTC
Cuts at: 384 384
Size: 5027
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI BaeI BfrBI
BmtI BsiWI BsmI BsmBI BspEI BssHII BstZ17I CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI