Restriction map for
pF1KA0580
(Circular) (Six-base) MAPSORT of: pF1KA0580.seq Check: 4633 from: 1 to: 8225
pF1KA0580 8225 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4290 4290
Size: 8225
AccI GT'mk_AC
Cuts at: 2923 5263 2923
Size: 2340 5885
Acc65I G'GTAC_C
Cuts at: 5241 5241
Size: 8225
AclI AA'CG_TT
Cuts at: 4758 8065 4758
Size: 3307 4918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 5051 6050 6482 7229 7497 2822
Size: 2229 999 432 747 268 3550
Fragments arranged by size:
3550 2229 999 747 432 268
AflII C'TTAA_G
Cuts at: 3285 3285
Size: 8225
AflIII A'CryG_T
Cuts at: 3042 5577 6681 7658 3042
Size: 2535 1104 977 3609
Fragments arranged by size:
3609 2535 1104 977
AgeI A'CCGG_T
Cuts at: 4481 6613 4481
Size: 2132 6093
AleI CACnn'nnGTG
Cuts at: 4388 4388
Size: 8225
AlwNI CAG_nnn'CTG
Cuts at: 748 1210 2204 2230 3145 5029 5530 7097 748
Size: 462 994 26 915 1884 501 1567 1876
Fragments arranged by size:
1884 1876 1567 994 915 501 462 26
ApaI G_GGCC'C
Cuts at: 3008 3008
Size: 8225
ApaLI G'TGCA_C
Cuts at: 6995 6995
Size: 8225
ApoI r'AATT_y
Cuts at: 591 789 1071 1086 1140 1168 1392 1624 1732
Size: 198 282 15 54 28 224 232 108
Cuts at: 1732 1913 2500 2698 2716 5229 7578 7834 591
Size: 181 587 198 18 2513 2349 256 982
Fragments arranged by size:
2513 2349 982 587 282 256 232 224
198 198 181 108 54 28 18 15
AseI AT'TA_AT
Cuts at: 20 161 2945 3977 20
Size: 141 2784 1032 4268
Fragments arranged by size:
4268 2784 1032 141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8225
AvaI C'yCGr_G
Cuts at: 1301 5245 1301
Size: 3944 4281
BamHI G'GATC_C
Cuts at: 5250 5250
Size: 8225
BanI G'GyrC_C
Cuts at: 2938 4652 5241 5655 5903 5938 2938
Size: 1714 589 414 248 35 5225
Fragments arranged by size:
5225 1714 589 414 248 35
BanII G_rGCy'C
Cuts at: 3008 3722 5239 6269 3008
Size: 714 1517 1030 4964
Fragments arranged by size:
4964 1517 1030 714
BbeI G_GCGC'C
Cuts at: 5659 5907 5659
Size: 248 7977
BbsI GAAGACnn'nnnn_
Cuts at: 497 667 2765 3297 5089 497
Size: 170 2098 532 1792 3633
Fragments arranged by size:
3633 2098 1792 532 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4288 4322 4288
Size: 34 8191
BciVI GTATCCnnnnn_n'
Cuts at: 278 533 1289 6123 6884 8225 278
Size: 255 756 4834 761 1341 278
Fragments arranged by size:
4834 1341 761 756 278 255
BclI T'GATC_A
Cuts at: 402 710 5121 5745 402
Size: 308 4411 624 2882
Fragments arranged by size:
4411 2882 624 308
BglI GCCn_nnn'nGGC
Cuts at: 5063 5063
Size: 8225
BglII A'GATC_T
Cuts at: 2290 3023 5740 7777 2290
Size: 733 2717 2037 2738
Fragments arranged by size:
2738 2717 2037 733
BlpI GC'TnA_GC
Cuts at: 3475 5337 3475
Size: 1862 6363
Bme1580I G_kGCm'C
Cuts at: 3008 5850 5943 6999 3008
Size: 2842 93 1056 4234
Fragments arranged by size:
4234 2842 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2244 2772 3011 4414 5437 5518 5853 7997 2244
Size: 528 239 1403 1023 81 335 2144 2472
Fragments arranged by size:
2472 2144 1403 1023 528 335 239 81
BmtI G_CTAG'C
Cuts at: 2342 2668 2342
Size: 326 7899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1207 6592 1207
Size: 5385 2840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4103 4315 4975 5035 3616
Size: 487 212 660 60 6806
Fragments arranged by size:
6806 660 487 212 60
Bpu10I CC'TnA_GC
Cuts at: 1636 1636
Size: 8225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 1838 2919 3056 5401 6280 6772 7070 1574
Size: 264 1081 137 2345 879 492 298 2729
Fragments arranged by size:
2729 2345 1081 879 492 298 264 137
BsaI GGTCTCn'nnnn_
Cuts at: 1108 2630 7929 1108
Size: 1522 5299 1404
Fragments arranged by size:
5299 1522 1404
BsaAI yAC'GTr
Cuts at: 5578 5578
Size: 8225
BsaBI GATnn'nnATC
Cuts at: 6 5739 7776 6
Size: 5733 2037 455
Fragments arranged by size:
5733 2037 455
BsaHI Gr'CG_yC
Cuts at: 5656 5904 6606 8102 5656
Size: 248 702 1496 5779
Fragments arranged by size:
5779 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2574 2632 4394 4481 5414 5935 6613 6887 7034
Size: 58 1762 87 933 521 678 274 147
Cuts at: 7034 7445 2574
Size: 411 3354
Fragments arranged by size:
3354 1762 933 678 521 411 274 147
87 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2603 2627 2633 2657 3019 3049 2603
Size: 24 6 24 362 30 7779
Fragments arranged by size:
7779 362 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 539 1096 1577 1801 539
Size: 557 481 224 6963
Fragments arranged by size:
6963 557 481 224
BseYI C'CCAG_C
Cuts at: 2677 4616 4712 5650 6408 6985 7481 2677
Size: 1939 96 938 758 577 496 3421
Fragments arranged by size:
3421 1939 938 758 577 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 2149 2228 2297 242
Size: 1907 79 69 6170
Fragments arranged by size:
6170 1907 79 69
BsiEI CG_ry'CG
Cuts at: 104 5767 5813 7021 104
Size: 5663 46 1208 1308
Fragments arranged by size:
5663 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3722 4961 5239 6017 6207 6999 3722
Size: 1239 278 778 190 792 4948
Fragments arranged by size:
4948 1239 792 778 278 190
BsmI GAATG_Cn'
Cuts at: 757 1372 3284 3891 4827 757
Size: 615 1912 607 936 4155
Fragments arranged by size:
4155 1912 936 615 607
Bsp1286I G_dGCh'C
Cuts at: 3008 3722 4961 5239 5850 5943 6017 6207 6269
Size: 714 1239 278 611 93 74 190 62
Cuts at: 6269 6999 3008
Size: 730 4234
Fragments arranged by size:
4234 1239 730 714 611 278 190 93
74 62
BspEI T'CCGG_A
Cuts at: 7445 7445
Size: 8225
BspHI T'CATG_A
Cuts at: 7401 7401
Size: 8225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3430 4290 5275 5489 5791 6172 3430
Size: 860 985 214 302 381 5483
Fragments arranged by size:
5483 985 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6517 6571 8055 8220 50
Size: 6467 54 1484 165 55
Fragments arranged by size:
6467 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2358 6137 2358
Size: 3779 4446
BsrFI r'CCGG_y
Cuts at: 4481 6223 6404 6613 4481
Size: 1742 181 209 6093
Fragments arranged by size:
6093 1742 209 181
BssSI C'ACGA_G
Cuts at: 6496 6854 6496
Size: 358 7867
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 2688 5602 1210
Size: 1478 2914 3833
Fragments arranged by size:
3833 2914 1478
BstBI TT'CG_AA
Cuts at: 1675 4977 6586 1675
Size: 3302 1609 3314
Fragments arranged by size:
3314 3302 1609
BstEII G'GTnAC_C
Cuts at: 271 271
Size: 8225
BstXI CCAn_nnnn'nTGG
Cuts at: 196 4616 196
Size: 4420 3805
BstYI r'GATC_y
Cuts at: 1 2226 2290 3023 3734 5250 5717 5740 6075
Size: 2225 64 733 711 1516 467 23 335
Cuts at: 6075 6321 7333 7419 7431 7777 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2225 1516 1012 733 711 467 449 346
335 246 86 64 23 12
BtgI C'CryG_G
Cuts at: 3384 4907 4937 3384
Size: 1523 30 6672
Fragments arranged by size:
6672 1523 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6175 6356 6519 6175
Size: 181 163 7881
Fragments arranged by size:
7881 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1331 3703 4383 4395 4947 5468 569
Size: 762 2372 680 12 552 521 3326
Fragments arranged by size:
3326 2372 762 680 552 521 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 446 481 446
Size: 35 8190
DraI TTT'AAA
Cuts at: 2996 3627 5224 2996
Size: 631 1597 5997
Fragments arranged by size:
5997 1597 631
DrdI GACnn_nn'nnGTC
Cuts at: 5931 6789 5931
Size: 858 7367
EaeI y'GGCC_r
Cuts at: 4732 5810 5984 6375 6402 7747 4732
Size: 1078 174 391 27 1345 5210
Fragments arranged by size:
5210 1345 1078 391 174 27
EagI C'GGCC_G
Cuts at: 5810 5810
Size: 8225
EarI CTCTTCn'nnn_
Cuts at: 1129 1778 2841 2852 3175 3806 4250 4277 4348
Size: 649 1063 11 323 631 444 27 71
Cuts at: 4348 4525 6248 6458 1129
Size: 177 1723 210 2896
Fragments arranged by size:
2896 1723 1063 649 631 444 323 210
177 71 27 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6433 6743 6889 8035 6433
Size: 310 146 1146 6623
Fragments arranged by size:
6623 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3720 5237 3720
Size: 1517 6708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3616 4103 4315 4975 5035 5051 6050 6482
Size: 794 487 212 660 60 16 999 432
Cuts at: 6482 7229 7497 2822
Size: 747 268 3550
Fragments arranged by size:
3550 999 794 747 660 487 432 268
212 60 16
EcoNI CCTnn'n_nnAGG
Cuts at: 4214 4214
Size: 8225
EcoO109I rG'GnC_Cy
Cuts at: 3969 5364 3969
Size: 1395 6830
EcoRI G'AATT_C
Cuts at: 1086 5229 1086
Size: 4143 4082
EcoRV GAT'ATC
Cuts at: 1885 4148 1885
Size: 2263 5962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4377 5056 6244 4377
Size: 679 1188 6358
Fragments arranged by size:
6358 1188 679
FspI TGC'GCA
Cuts at: 6006 6006
Size: 8225
HaeII r_GCGC'y
Cuts at: 5659 5907 6929 7908 5659
Size: 248 1022 979 5976
Fragments arranged by size:
5976 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 654 686 1075 1107 1567 1599 2224
Size: 32 93 32 389 32 460 32 625
Cuts at: 2224 2256 2603 2635 4542 4574 4888 4920 6067
Size: 32 347 32 1907 32 314 32 1147
Cuts at: 6067 6097 6099 6129 529
Size: 30 2 30 2625
Fragments arranged by size:
2625 1907 1147 625 460 389 347 314
93 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 992 3166 3813 5264 992
Size: 2174 647 1451 3953
Fragments arranged by size:
3953 2174 1451 647
HindIII A'AGCT_T
Cuts at: 3621 3621
Size: 8225
KasI G'GCGC_C
Cuts at: 5655 5903 5655
Size: 248 7977
KpnI G_GTAC'C
Cuts at: 5245 5245
Size: 8225
MfeI C'AATT_G
Cuts at: 3425 3425
Size: 8225
MluI A'CGCG_T
Cuts at: 7658 7658
Size: 8225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 2219 3380 4633 6896 7080 446
Size: 1773 1161 1253 2263 184 1591
Fragments arranged by size:
2263 1773 1591 1253 1161 184
MscI TGG'CCA
Cuts at: 4734 5986 4734
Size: 1252 6973
MslI CAynn'nnrTG
Cuts at: 188 3499 3584 3884 4388 4721 6214 6341 188
Size: 3311 85 300 504 333 1493 127 2072
Fragments arranged by size:
3311 2072 1493 504 333 300 127 85
MspA1I CmG'CkG
Cuts at: 3509 4369 4741 5336 5650 6010 7023 7268 3509
Size: 860 372 595 314 360 1013 245 4466
Fragments arranged by size:
4466 1013 860 595 372 360 314 245
NaeI GCC'GGC
Cuts at: 6406 6406
Size: 8225
NarI GG'CG_CC
Cuts at: 5656 5904 5656
Size: 248 7977
NcoI C'CATG_G
Cuts at: 3384 4937 3384
Size: 1553 6672
NdeI CA'TA_TG
Cuts at: 2441 2441
Size: 8225
NgoMIV G'CCGG_C
Cuts at: 6404 6404
Size: 8225
NheI G'CTAG_C
Cuts at: 2338 2664 2338
Size: 326 7899
NspI r_CATG'y
Cuts at: 1605 5278 5778 6685 1605
Size: 3673 500 907 3145
Fragments arranged by size:
3673 3145 907 500
PasI CC'CwG_GG
Cuts at: 1179 1179
Size: 8225
PciI A'CATG_T
Cuts at: 6681 6681
Size: 8225
PflMI CCAn_nnn'nTGG
Cuts at: 3192 3897 4741 4953 3192
Size: 705 844 212 6464
Fragments arranged by size:
6464 844 705 212
PfoI T'CCnGG_A
Cuts at: 3593 3593
Size: 8225
PmeI GTTT'AAAC
Cuts at: 5224 5224
Size: 8225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7364 7396 7364
Size: 32 8193
PpuMI rG'GwC_Cy
Cuts at: 3969 3969
Size: 8225
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 8225
PspOMI G'GGCC_C
Cuts at: 3004 3004
Size: 8225
PstI C_TGCA'G
Cuts at: 248 576 4390 5272 5957 248
Size: 328 3814 882 685 2516
Fragments arranged by size:
3814 2516 882 685 328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8225
PvuII CAG'CTG
Cuts at: 3509 4741 5650 6010 3509
Size: 1232 909 360 5724
Fragments arranged by size:
5724 1232 909 360
RsrII CG'GwC_CG
Cuts at: 6420 6420
Size: 8225
SacI G_AGCT'C
Cuts at: 3722 5239 3722
Size: 1517 6708
SalI G'TCGA_C
Cuts at: 5262 5262
Size: 8225
SapI GCTCTTCn'nnn_
Cuts at: 4250 6248 6458 4250
Size: 1998 210 6017
Fragments arranged by size:
6017 1998 210
SbfI CC_TGCA'GG
Cuts at: 5272 5272
Size: 8225
ScaI AGT'ACT
Cuts at: 7742 7742
Size: 8225
SexAI A'CCwGG_T
Cuts at: 4343 4343
Size: 8225
SfcI C'TryA_G
Cuts at: 33 244 266 572 2067 2564 4128 4386 5268
Size: 211 22 306 1495 497 1564 258 882
Cuts at: 5268 5953 6946 7137 33
Size: 685 993 191 1121
Fragments arranged by size:
1564 1495 1121 993 882 685 497 306
258 211 191 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 5063 5063
Size: 8225
SfoI GGC'GCC
Cuts at: 5657 5905 5657
Size: 248 7977
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8225
SmaI CCC'GGG
Cuts at: 5247 5247
Size: 8225
SmlI C'TyrA_G
Cuts at: 1301 1589 1853 2898 3071 3285 5380 6295 6787
Size: 288 264 1045 173 214 2095 915 492
Cuts at: 6787 7049 1301
Size: 262 2477
Fragments arranged by size:
2477 2095 1045 915 492 288 264 262
214 173
SpeI A'CTAG_T
Cuts at: 67 3943 67
Size: 3876 4349
SphI G_CATG'C
Cuts at: 5278 5778 5278
Size: 500 7725
SspI AAT'ATT
Cuts at: 633 3995 4203 4845 633
Size: 3362 208 642 4013
Fragments arranged by size:
4013 3362 642 208
StuI AGG'CCT
Cuts at: 5066 5066
Size: 8225
StyI C'CwwG_G
Cuts at: 2312 3384 3902 4829 4937 5359 5713 2312
Size: 1072 518 927 108 422 354 4824
Fragments arranged by size:
4824 1072 927 518 422 354 108
SwaI ATTT'AAAT
Cuts at: 2996 2996
Size: 8225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1608 2045 4081 5808 5941 6400 6609 1608
Size: 437 2036 1727 133 459 209 3224
Fragments arranged by size:
3224 2036 1727 459 437 209 133
TatI w'GTAC_w
Cuts at: 4290 4399 7740 4290
Size: 109 3341 4775
Fragments arranged by size:
4775 3341 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1925 1957 2627 2659 7742 7774 1925
Size: 32 670 32 5083 32 2376
Fragments arranged by size:
5083 2376 670 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6022 6022
Size: 8225
XbaI T'CTAG_A
Cuts at: 2583 5256 2583
Size: 2673 5552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 5720 2231
Size: 3489 4736
XhoI C'TCGA_G
Cuts at: 1301 1301
Size: 8225
XmaI C'CCGG_G
Cuts at: 5245 5245
Size: 8225
XmnI GAAnn'nnTTC
Cuts at: 789 1370 1392 4357 4758 789
Size: 581 22 2965 401 4256
Fragments arranged by size:
4256 2965 581 401 22
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII FseI FspAI HpaI NotI NruI
NsiI PacI PmlI PshAI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI ZraI