Restriction map for
pF1KA0583
(Circular) (Six-base) MAPSORT of: pF1KA0583.seq Check: 2831 from: 1 to: 8870
pF1KA0583 8870 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 359 960 1329 3377 3747 359
Size: 601 369 2048 370 5482
Fragments arranged by size:
5482 2048 601 370 369
AccI GT'mk_AC
Cuts at: 5474 5908 5474
Size: 434 8436
Acc65I G'GTAC_C
Cuts at: 4450 5886 4450
Size: 1436 7434
AclI AA'CG_TT
Cuts at: 8710 8710
Size: 8870
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 847 1174 1654 1864 2506 2942 3365 4831
Size: 147 327 480 210 642 436 423 1466
Cuts at: 4831 4855 5132 6695 7127 7874 8142 700
Size: 24 277 1563 432 747 268 1428
Fragments arranged by size:
1563 1466 1428 747 642 480 436 432
423 327 277 268 210 147 24
AfeI AGC'GCT
Cuts at: 212 212
Size: 8870
AflII C'TTAA_G
Cuts at: 2112 2112
Size: 8870
AflIII A'CryG_T
Cuts at: 4608 6222 7326 8303 4608
Size: 1614 1104 977 5175
Fragments arranged by size:
5175 1614 1104 977
AgeI A'CCGG_T
Cuts at: 7258 7258
Size: 8870
AhdI GACnn_n'nnGTC
Cuts at: 2421 4041 2421
Size: 1620 7250
AleI CACnn'nnGTG
Cuts at: 1339 2080 2974 3220 3667 4655 1339
Size: 741 894 246 447 988 5554
Fragments arranged by size:
5554 988 894 741 447 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4327 4327
Size: 8870
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1876 1908 1876
Size: 32 8838
AlwNI CAG_nnn'CTG
Cuts at: 153 318 720 870 1632 2920 2980 3091 3504
Size: 165 402 150 762 1288 60 111 413
Cuts at: 3504 3824 4290 5155 6175 7742 153
Size: 320 466 865 1020 1567 1281
Fragments arranged by size:
1567 1288 1281 1020 865 762 466 413
402 320 165 150 111 60
ApaI G_GGCC'C
Cuts at: 2881 4908 2881
Size: 2027 6843
ApaLI G'TGCA_C
Cuts at: 4399 4648 7640 4399
Size: 249 2992 5629
Fragments arranged by size:
5629 2992 249
ApoI r'AATT_y
Cuts at: 5086 5874 8223 8479 5086
Size: 788 2349 256 5477
Fragments arranged by size:
5477 2349 788 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8870
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8870
AvaI C'yCGr_G
Cuts at: 187 196 578 2052 3249 3277 3746 4111 4270
Size: 9 382 1474 1197 28 469 365 159
Cuts at: 4270 5890 187
Size: 1620 3167
Fragments arranged by size:
3167 1620 1474 1197 469 382 365 159
28 9
BamHI G'GATC_C
Cuts at: 5895 5895
Size: 8870
BanI G'GyrC_C
Cuts at: 256 495 3564 4450 4477 5886 6300 6548 6583
Size: 239 3069 886 27 1409 414 248 35
Cuts at: 6583 256
Size: 2543
Fragments arranged by size:
3069 2543 1409 886 414 248 239 35
27
BanII G_rGCy'C
Cuts at: 116 596 1853 2506 2574 2788 2881 3287 3554
Size: 480 1257 653 68 214 93 406 267
Cuts at: 3554 3676 3973 4177 4908 5784 5884 6914 116
Size: 122 297 204 731 876 100 1030 2072
Fragments arranged by size:
2072 1257 1030 876 731 653 480 406
297 267 214 204 122 100 93 68
BbeI G_GCGC'C
Cuts at: 260 499 6304 6552 260
Size: 239 5805 248 2578
Fragments arranged by size:
5805 2578 248 239
BbsI GAAGACnn'nnnn_
Cuts at: 343 1351 2672 4600 4608 343
Size: 1008 1321 1928 8 4605
Fragments arranged by size:
4605 1928 1321 1008 8
BbvCI CC'TCA_GC
Cuts at: 469 847 1784 4068 469
Size: 378 937 2284 5271
Fragments arranged by size:
5271 2284 937 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1340 1374 1592 1626 3240 3274 3418 3452 3554
Size: 34 218 34 1614 34 144 34 102
Cuts at: 3554 3588 3888 3922 4179 4213 1340
Size: 34 300 34 257 34 5997
Fragments arranged by size:
5997 1614 300 257 218 144 102 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6768 7529 8870 6768
Size: 761 1341 6768
Fragments arranged by size:
6768 1341 761
BclI T'GATC_A
Cuts at: 6390 6390
Size: 8870
BglI GCCn_nnn'nGGC
Cuts at: 2185 2185
Size: 8870
BglII A'GATC_T
Cuts at: 5804 6385 8422 5804
Size: 581 2037 6252
Fragments arranged by size:
6252 2037 581
BlpI GC'TnA_GC
Cuts at: 2962 4408 5101 5982 2962
Size: 1446 693 881 5850
Fragments arranged by size:
5850 1446 881 693
Bme1580I G_kGCm'C
Cuts at: 484 960 2881 3567 3637 4403 4652 4908 4996
Size: 476 1921 686 70 766 249 256 88
Cuts at: 4996 6495 6588 7644 484
Size: 1499 93 1056 1710
Fragments arranged by size:
1921 1710 1499 1056 766 686 476 256
249 93 88 70
BmgBI CAC'GTC
Cuts at: 837 2835 3270 4770 5358 837
Size: 1998 435 1500 588 4349
Fragments arranged by size:
4349 1998 1500 588 435
BmrI ACTGGGnnnn_n'
Cuts at: 132 1689 1866 2020 2756 3452 3770 4377 6082
Size: 1557 177 154 736 696 318 607 1705
Cuts at: 6082 6163 6498 8642 132
Size: 81 335 2144 360
Fragments arranged by size:
2144 1705 1557 736 696 607 360 335
318 177 154 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 570 3006 3216 4271 7237 570
Size: 2436 210 1055 2966 2203
Fragments arranged by size:
2966 2436 2203 1055 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1141 1630 2329 3211 3464 3526 3538 3861
Size: 789 489 699 882 253 62 12 323
Cuts at: 3861 4312 4450 4551 4621 4657 4771 5076 352
Size: 451 138 101 70 36 114 305 4146
Fragments arranged by size:
4146 882 789 699 489 451 323 305
253 138 114 101 70 62 36 12
Bpu10I CC'TnA_GC
Cuts at: 469 847 1111 1784 2782 3799 3811 4068 469
Size: 378 264 673 998 1017 12 257 5271
Fragments arranged by size:
5271 1017 998 673 378 264 257 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1471 1837 5434 5755 6046 6925 7417 7715
Size: 1209 366 3597 321 291 879 492 298
Cuts at: 7715 262
Size: 1417
Fragments arranged by size:
3597 1417 1209 879 492 366 321 298
291
BsaI GGTCTCn'nnnn_
Cuts at: 2860 5657 8574 2860
Size: 2797 2917 3156
Fragments arranged by size:
3156 2917 2797
BsaAI yAC'GTr
Cuts at: 4158 4398 4971 6223 4158
Size: 240 573 1252 6805
Fragments arranged by size:
6805 1252 573 240
BsaBI GATnn'nnATC
Cuts at: 6 6384 8421 6
Size: 6378 2037 455
Fragments arranged by size:
6378 2037 455
BsaHI Gr'CG_yC
Cuts at: 257 377 496 1061 3795 4826 5518 6301 6549
Size: 120 119 565 2734 1031 692 783 248
Cuts at: 6549 7251 8747 257
Size: 702 1496 380
Fragments arranged by size:
2734 1496 1031 783 702 692 565 380
248 120 119
BsaWI w'CCGG_w
Cuts at: 1727 6059 6580 7258 7532 7679 8090 1727
Size: 4332 521 678 274 147 411 2507
Fragments arranged by size:
4332 2507 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 497 527 1876 1906 2611 2641 3388 3418 4924
Size: 30 1349 30 705 30 747 30 1506
Cuts at: 4924 4954 5540 5570 497
Size: 30 586 30 3797
Fragments arranged by size:
3797 1506 1349 747 705 586 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 505 772 940 1150 1242 1390 1537 2182
Size: 246 267 168 210 92 148 147 645
Cuts at: 2182 2496 3351 4001 4159 4870 5792 259
Size: 314 855 650 158 711 922 3337
Fragments arranged by size:
3337 922 855 711 650 645 314 267
246 210 168 158 148 147 92
BseYI C'CCAG_C
Cuts at: 116 959 1128 1175 1570 3110 3423 3658 4185
Size: 843 169 47 395 1540 313 235 527
Cuts at: 4185 4548 4631 5062 5432 6295 7053 7630 8126
Size: 363 83 431 370 863 758 577 496
Cuts at: 8126 116
Size: 860
Fragments arranged by size:
1540 863 860 843 758 577 527 496
431 395 370 363 313 235 169 83
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2251 4186 4861 5141 5298 2251
Size: 1935 675 280 157 5823
Fragments arranged by size:
5823 1935 675 280 157
BsiEI CG_ry'CG
Cuts at: 104 3469 4917 6412 6458 7666 104
Size: 3365 1448 1495 46 1208 1308
Fragments arranged by size:
3365 1495 1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 596 1853 2506 2788 3554 3676 4403 4652
Size: 408 1257 653 282 766 122 727 249
Cuts at: 4652 5884 6662 6852 7644 188
Size: 1232 778 190 792 1414
Fragments arranged by size:
1414 1257 1232 792 778 766 727 653
408 282 249 190 122
BsmI GAATG_Cn'
Cuts at: 3582 4006 5037 5700 3582
Size: 424 1031 663 6752
Fragments arranged by size:
6752 1031 663 424
BsmBI CGTCTCn'nnnn_
Cuts at: 1978 4233 1978
Size: 2255 6615
Bsp1286I G_dGCh'C
Cuts at: 116 188 484 596 960 1853 2506 2574 2788
Size: 72 296 112 364 893 653 68 214
Cuts at: 2788 2881 3287 3554 3567 3637 3676 3973 4177
Size: 93 406 267 13 70 39 297 204
Cuts at: 4177 4403 4652 4908 4996 5784 5884 6495 6588
Size: 226 249 256 88 788 100 611 93
Cuts at: 6588 6662 6852 6914 7644 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 893 788 730 653 611 406 364
297 296 267 256 249 226 214 204
190 112 100 93 93 88 74 72
70 68 62 39 13
BspEI T'CCGG_A
Cuts at: 1727 8090 1727
Size: 6363 2507
BspHI T'CATG_A
Cuts at: 1412 8046 1412
Size: 6634 2236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 359 719 893 960 1292 1329 2730 3086
Size: 33 360 174 67 332 37 1401 356
Cuts at: 3086 3377 3747 3832 4319 4676 5231 5741 5920
Size: 291 370 85 487 357 555 510 179
Cuts at: 5920 6134 6436 6817 326
Size: 214 302 381 2379
Fragments arranged by size:
2379 1401 555 510 487 381 370 360
357 356 332 302 291 214 179 174
85 67 37 33
BsrBI CCG'CTC
Cuts at: 50 228 300 1380 1758 2795 4119 5320 5448
Size: 178 72 1080 378 1037 1324 1201 128
Cuts at: 5448 5586 5862 7162 7216 8700 8865 50
Size: 138 276 1300 54 1484 165 55
Fragments arranged by size:
1484 1324 1300 1201 1080 1037 378 276
178 165 138 128 72 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2405 3323 4078 5564 5819 6782 2096
Size: 309 918 755 1486 255 963 4184
Fragments arranged by size:
4184 1486 963 918 755 309 255
BsrFI r'CCGG_y
Cuts at: 524 800 1670 2843 3372 3440 3590 4447 4916
Size: 276 870 1173 529 68 150 857 469
Cuts at: 4916 5162 6868 7049 7258 524
Size: 246 1706 181 209 2136
Fragments arranged by size:
2136 1706 1173 870 857 529 469 276
246 209 181 150 68
BsrGI T'GTAC_A
Cuts at: 1391 1391
Size: 8870
BssSI C'ACGA_G
Cuts at: 922 2508 4729 7141 7499 922
Size: 1586 2221 2412 358 2293
Fragments arranged by size:
2412 2293 2221 1586 358
BstAPI GCAn_nnn'nTGC
Cuts at: 720 870 5399 6247 720
Size: 150 4529 848 3343
Fragments arranged by size:
4529 3343 848 150
BstBI TT'CG_AA
Cuts at: 1602 7231 1602
Size: 5629 3241
BstEII G'GTnAC_C
Cuts at: 946 4236 946
Size: 3290 5580
BstXI CCAn_nnnn'nTGG
Cuts at: 2217 3222 4474 2217
Size: 1005 1252 6613
Fragments arranged by size:
6613 1252 1005
BstYI r'GATC_y
Cuts at: 1 683 5042 5804 5895 6362 6385 6720 6966
Size: 682 4359 762 91 467 23 335 246
Cuts at: 6966 7978 8064 8076 8422 1
Size: 1012 86 12 346 449
Fragments arranged by size:
4359 1012 762 682 467 449 346 335
246 91 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1225 1747 4853 5677 1225
Size: 522 3106 824 4418
Fragments arranged by size:
4418 3106 824 522
BtgI C'CryG_G
Cuts at: 107 203 1025 1265 1946 2574 2814 2969 3855
Size: 96 822 240 681 628 240 155 886
Cuts at: 3855 3955 4241 4322 107
Size: 100 286 81 4655
Fragments arranged by size:
4655 886 822 681 628 286 240 240
155 100 96 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4236 6820 7001 7164 4236
Size: 2584 181 163 5942
Fragments arranged by size:
5942 2584 181 163
BtsI GCAGTG_nn'
Cuts at: 480 1294 1615 2851 2981 3868 4363 4479 5550
Size: 814 321 1236 130 887 495 116 1071
Cuts at: 5550 6113 480
Size: 563 3237
Fragments arranged by size:
3237 1236 1071 887 814 563 495 321
130 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2262 2297 2262
Size: 35 8835
DraI TTT'AAA
Cuts at: 5869 5869
Size: 8870
DraIII CAC_nnn'GTG
Cuts at: 1738 1738
Size: 8870
DrdI GACnn_nn'nnGTC
Cuts at: 6576 7434 6576
Size: 858 8012
EaeI y'GGCC_r
Cuts at: 290 764 1022 1190 1472 1514 1661 1928 2106
Size: 474 258 168 282 42 147 267 178
Cuts at: 2106 2517 2841 2848 3374 3442 3466 3527 4685
Size: 411 324 7 526 68 24 61 1158
Cuts at: 4685 5493 6455 6629 7020 7047 8392 290
Size: 808 962 174 391 27 1345 768
Fragments arranged by size:
1345 1158 962 808 768 526 474 411
391 324 282 267 258 178 174 168
147 68 61 42 27 24 7
EagI C'GGCC_G
Cuts at: 3466 6455 3466
Size: 2989 5881
EarI CTCTTCn'nnn_
Cuts at: 742 1066 6893 7103 742
Size: 324 5827 210 2509
Fragments arranged by size:
5827 2509 324 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 280 1669 2244 3934 4819 7078 7388 7534 8680
Size: 1389 575 1690 885 2259 310 146 1146
Cuts at: 8680 280
Size: 470
Fragments arranged by size:
2259 1690 1389 1146 885 575 470 310
146
EcoICRI GAG'CTC
Cuts at: 594 1851 2504 2786 3552 3674 5882 594
Size: 1257 653 282 766 122 2208 3582
Fragments arranged by size:
3582 2208 1257 766 653 282 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 700 847 1141 1174 1630 1654 1864 2329
Size: 348 147 294 33 456 24 210 465
Cuts at: 2329 2506 2942 3211 3365 3464 3526 3538 3861
Size: 177 436 269 154 99 62 12 323
Cuts at: 3861 4312 4450 4551 4621 4657 4771 4831 4855
Size: 451 138 101 70 36 114 60 24
Cuts at: 4855 5076 5132 6695 7127 7874 8142 352
Size: 221 56 1563 432 747 268 1080
Fragments arranged by size:
1563 1080 747 465 456 451 436 432
348 323 294 269 268 221 210 177
154 147 138 114 101 99 70 62
60 56 36 33 24 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 157 651 747 1648 2485 2908 3120 157
Size: 494 96 901 837 423 212 5907
Fragments arranged by size:
5907 901 837 494 423 212 96
EcoO109I rG'GnC_Cy
Cuts at: 315 644 1281 1788 2366 2683 2731 2878 2942
Size: 329 637 507 578 317 48 147 64
Cuts at: 2942 3088 3130 3396 3821 4534 4717 4740 4875
Size: 146 42 266 425 713 183 23 135
Cuts at: 4875 4904 4905 5003 5344 6009 315
Size: 29 1 98 341 665 3176
Fragments arranged by size:
3176 713 665 637 578 507 425 341
329 317 266 183 147 146 135 98
64 48 42 29 23 1
EcoRI G'AATT_C
Cuts at: 5086 5874 5086
Size: 788 8082
EcoRV GAT'ATC
Cuts at: 3952 3952
Size: 8870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 2386 3042 6889 303
Size: 2083 656 3847 2284
Fragments arranged by size:
3847 2284 2083 656
FspI TGC'GCA
Cuts at: 1795 3706 6651 1795
Size: 1911 2945 4014
Fragments arranged by size:
4014 2945 1911
HaeII r_GCGC'y
Cuts at: 123 214 260 331 499 606 1537 2191 3257
Size: 91 46 71 168 107 931 654 1066
Cuts at: 3257 3884 4322 5135 5642 6304 6552 7574 8553
Size: 627 438 813 507 662 248 1022 979
Cuts at: 8553 123
Size: 440
Fragments arranged by size:
1066 1022 979 931 813 662 654 627
507 440 438 248 168 107 91 71
46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 2372 2404 2407 2439 3343 3375 3388
Size: 32 2010 32 3 32 904 32 13
Cuts at: 3388 3420 3480 3512 4027 4059 4483 4515 5540
Size: 32 60 32 515 32 424 32 1025
Cuts at: 5540 5572 5654 5686 6712 6742 6744 6774 330
Size: 32 82 32 1026 30 2 30 2426
Fragments arranged by size:
2426 2010 1026 1025 904 515 424 82
60 32 32 32 32 32 32 32
32 32 32 30 30 13 3 2
HincII GTy'rAC
Cuts at: 4098 5475 5909 4098
Size: 1377 434 7059
Fragments arranged by size:
7059 1377 434
HindIII A'AGCT_T
Cuts at: 3472 5731 3472
Size: 2259 6611
KasI G'GCGC_C
Cuts at: 256 495 6300 6548 256
Size: 239 5805 248 2578
Fragments arranged by size:
5805 2578 248 239
KpnI G_GTAC'C
Cuts at: 4454 5890 4454
Size: 1436 7434
MluI A'CGCG_T
Cuts at: 4608 8303 4608
Size: 3695 5175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 686 1016 1340 1856 1920 2954 3887 7541 7725
Size: 330 324 516 64 1034 933 3654 184
Cuts at: 7725 686
Size: 1831
Fragments arranged by size:
3654 1831 1034 933 516 330 324 184
64
MscI TGG'CCA
Cuts at: 292 766 1663 2519 6631 292
Size: 474 897 856 4112 2531
Fragments arranged by size:
4112 2531 897 856 474
MslI CAynn'nnrTG
Cuts at: 1024 1339 2080 2215 2221 2974 3220 3667 3776
Size: 315 741 135 6 753 246 447 109
Cuts at: 3776 4655 5501 6859 6986 1024
Size: 879 846 1358 127 2908
Fragments arranged by size:
2908 1358 879 846 753 741 447 315
246 135 127 109 6
MspA1I CmG'CkG
Cuts at: 205 237 564 867 1128 1146 1200 1902 2265
Size: 32 327 303 261 18 54 702 363
Cuts at: 2265 2332 2936 3957 4185 4635 5022 5152 5201
Size: 67 604 1021 228 450 387 130 49
Cuts at: 5201 5295 5981 6295 6655 7668 7913 205
Size: 94 686 314 360 1013 245 1162
Fragments arranged by size:
1162 1021 1013 702 686 604 450 387
363 360 327 314 303 261 245 228
130 94 67 54 49 32 18
NaeI GCC'GGC
Cuts at: 802 1672 2845 3374 3442 3592 7051 802
Size: 870 1173 529 68 150 3459 2621
Fragments arranged by size:
3459 2621 1173 870 529 150 68
NarI GG'CG_CC
Cuts at: 257 496 6301 6549 257
Size: 239 5805 248 2578
Fragments arranged by size:
5805 2578 248 239
NcoI C'CATG_G
Cuts at: 107 1025 1946 2574 3855 107
Size: 918 921 628 1281 5122
Fragments arranged by size:
5122 1281 921 918 628
NgoMIV G'CCGG_C
Cuts at: 800 1670 2843 3372 3440 3590 7049 800
Size: 870 1173 529 68 150 3459 2621
Fragments arranged by size:
3459 2621 1173 870 529 150 68
NspI r_CATG'y
Cuts at: 846 5109 5601 5923 6423 7330 846
Size: 4263 492 322 500 907 2386
Fragments arranged by size:
4263 2386 907 500 492 322
PasI CC'CwG_GG
Cuts at: 2241 2725 3762 4817 4989 2241
Size: 484 1037 1055 172 6122
Fragments arranged by size:
6122 1055 1037 484 172
PciI A'CATG_T
Cuts at: 7326 7326
Size: 8870
PflMI CCAn_nnn'nTGG
Cuts at: 1952 2725 3091 3414 5307 1952
Size: 773 366 323 1893 5515
Fragments arranged by size:
5515 1893 773 366 323
PfoI T'CCnGG_A
Cuts at: 311 1646 1688 3503 3554 4567 4748 5005 5092
Size: 1335 42 1815 51 1013 181 257 87
Cuts at: 5092 5558 311
Size: 466 3623
Fragments arranged by size:
3623 1815 1335 1013 466 257 181 87
51 42
PmeI GTTT'AAAC
Cuts at: 5869 5869
Size: 8870
PmlI CAC'GTG
Cuts at: 4158 4398 4158
Size: 240 8630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1876 1908 8009 8041 1876
Size: 32 6101 32 2705
Fragments arranged by size:
6101 2705 32 32
PpuMI rG'GwC_Cy
Cuts at: 315 1281 2366 2731 2942 3088 3396 3821 4717
Size: 966 1085 365 211 146 308 425 896
Cuts at: 4717 4740 5003 315
Size: 23 263 4182
Fragments arranged by size:
4182 1085 966 896 425 365 308 263
211 146 23
PsiI TTA'TAA
Cuts at: 2999 2999
Size: 8870
PspOMI G'GGCC_C
Cuts at: 2877 4904 2877
Size: 2027 6843
PspXI vC'TCGA_Gb
Cuts at: 196 4270 196
Size: 4074 4796
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3679 3711 3679
Size: 32 8838
PstI C_TGCA'G
Cuts at: 716 725 893 1205 1289 1310 1457 3730 3829
Size: 9 168 312 84 21 147 2273 99
Cuts at: 3829 5404 5917 6602 716
Size: 1575 513 685 2984
Fragments arranged by size:
2984 2273 1575 685 513 312 168 147
99 84 21 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8870
PvuII CAG'CTG
Cuts at: 237 564 867 1128 1200 1902 4635 5022 6295
Size: 327 303 261 72 702 2733 387 1273
Cuts at: 6295 6655 237
Size: 360 2452
Fragments arranged by size:
2733 2452 1273 702 387 360 327 303
261 72
RsrII CG'GwC_CG
Cuts at: 7065 7065
Size: 8870
SacI G_AGCT'C
Cuts at: 596 1853 2506 2788 3554 3676 5884 596
Size: 1257 653 282 766 122 2208 3582
Fragments arranged by size:
3582 2208 1257 766 653 282 122
SacII CC_GC'GG
Cuts at: 206 3958 206
Size: 3752 5118
SalI G'TCGA_C
Cuts at: 5473 5907 5473
Size: 434 8436
SanDI GG'GwC_CC
Cuts at: 2366 2731 2366
Size: 365 8505
SapI GCTCTTCn'nnn_
Cuts at: 6893 7103 6893
Size: 210 8660
SbfI CC_TGCA'GG
Cuts at: 5917 5917
Size: 8870
ScaI AGT'ACT
Cuts at: 8387 8387
Size: 8870
SexAI A'CCwGG_T
Cuts at: 1427 1427
Size: 8870
SfcI C'TryA_G
Cuts at: 33 420 712 721 889 1201 1285 1306 1453
Size: 387 292 9 168 312 84 21 147
Cuts at: 1453 3726 3825 5400 5913 6598 7591 7782 33
Size: 2273 99 1575 513 685 993 191 1121
Fragments arranged by size:
2273 1575 1121 993 685 513 387 312
292 191 168 147 99 84 21 9
SfoI GGC'GCC
Cuts at: 258 497 6302 6550 258
Size: 239 5805 248 2578
Fragments arranged by size:
5805 2578 248 239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8870
SmaI CCC'GGG
Cuts at: 580 2054 3279 5892 580
Size: 1474 1225 2613 3558
Fragments arranged by size:
3558 2613 1474 1225
SmlI C'TyrA_G
Cuts at: 196 277 1486 1852 2112 4270 5413 5734 6025
Size: 81 1209 366 260 2158 1143 321 291
Cuts at: 6025 6940 7432 7694 196
Size: 915 492 262 1372
Fragments arranged by size:
2158 1372 1209 1143 915 492 366 321
291 262 260 81
SpeI A'CTAG_T
Cuts at: 67 3241 67
Size: 3174 5696
SphI G_CATG'C
Cuts at: 5923 6423 5923
Size: 500 8370
StuI AGG'CCT
Cuts at: 2755 2899 4134 5328 5428 2755
Size: 144 1235 1194 100 6197
Fragments arranged by size:
6197 1235 1194 144 100
StyI C'CwwG_G
Cuts at: 107 260 293 767 1025 1946 2574 2951 3855
Size: 153 33 474 258 921 628 377 904
Cuts at: 3855 4232 4712 4871 5207 5231 6004 6358 107
Size: 377 480 159 336 24 773 354 2619
Fragments arranged by size:
2619 921 904 773 628 480 474 377
377 354 336 258 159 153 33 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1336 1474 2233 2884 3868 6453 6586 7045 7254
Size: 138 759 651 984 2585 133 459 209
Cuts at: 7254 1336
Size: 2952
Fragments arranged by size:
2952 2585 984 759 651 459 209 138
133
TatI w'GTAC_w
Cuts at: 1391 1400 4385 5744 8385 1391
Size: 9 2985 1359 2641 1876
Fragments arranged by size:
2985 2641 1876 1359 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 1273 1305 1722 1754 2611 2643 3502
Size: 32 744 32 417 32 857 32 859
Cuts at: 3502 3534 4408 4440 8387 8419 497
Size: 32 874 32 3947 32 948
Fragments arranged by size:
3947 948 874 859 857 744 417 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1429 2475 5145 6667 1429
Size: 1046 2670 1522 3632
Fragments arranged by size:
3632 2670 1522 1046
XbaI T'CTAG_A
Cuts at: 5901 5901
Size: 8870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 1032 1524 3885 4239 4374 4434 4639 6365
Size: 861 492 2361 354 135 60 205 1726
Cuts at: 6365 171
Size: 2676
Fragments arranged by size:
2676 2361 1726 861 492 354 205 135
60
XhoI C'TCGA_G
Cuts at: 196 4270 196
Size: 4074 4796
XmaI C'CCGG_G
Cuts at: 578 2052 3277 5890 578
Size: 1474 1225 2613 3558
Fragments arranged by size:
3558 2613 1474 1225
XmnI GAAnn'nnTTC
Cuts at: 1598 2491 1598
Size: 893 7977
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI AvrII BaeI BfrBI BmtI BsiWI BssHII
BstZ17I ClaI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI