Restriction map for
pF1KA0584
(Circular) (Six-base) MAPSORT of: pF1KA0584.seq Check: 6697 from: 1 to: 4991
pF1KA0584 4991 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2029 2029
Size: 4991
Acc65I G'GTAC_C
Cuts at: 1808 2007 1808
Size: 199 4792
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1285 2816 3248 3995 4263 1015
Size: 270 1531 432 747 268 1743
Fragments arranged by size:
1743 1531 747 432 270 268
AflIII A'CryG_T
Cuts at: 2343 3447 4424 2343
Size: 1104 977 2910
Fragments arranged by size:
2910 1104 977
AgeI A'CCGG_T
Cuts at: 3379 3379
Size: 4991
AlwNI CAG_nnn'CTG
Cuts at: 379 1011 1635 2296 3863 379
Size: 632 624 661 1567 1507
Fragments arranged by size:
1567 1507 661 632 624
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 1995 4344 4600 1995
Size: 2349 256 2386
Fragments arranged by size:
2386 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 692 2011 692
Size: 1319 3672
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1800 1833 1800
Size: 33 4958
BamHI G'GATC_C
Cuts at: 2016 2016
Size: 4991
BanI G'GyrC_C
Cuts at: 1808 2007 2421 2669 2704 1808
Size: 199 414 248 35 4095
Fragments arranged by size:
4095 414 248 199 35
BanII G_rGCy'C
Cuts at: 265 2005 3035 265
Size: 1740 1030 2221
Fragments arranged by size:
2221 1740 1030
BbeI G_GCGC'C
Cuts at: 2425 2673 2425
Size: 248 4743
BbsI GAAGACnn'nnnn_
Cuts at: 1025 1405 1421 1025
Size: 380 16 4595
Fragments arranged by size:
4595 380 16
BbvCI CC'TCA_GC
Cuts at: 159 159
Size: 4991
BciVI GTATCCnnnnn_n'
Cuts at: 2889 3650 4991 2889
Size: 761 1341 2889
Fragments arranged by size:
2889 1341 761
BclI T'GATC_A
Cuts at: 381 2511 381
Size: 2130 2861
BglI GCCn_nnn'nGGC
Cuts at: 370 370
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 1484 2103 1484
Size: 619 4372
Bme1580I G_kGCm'C
Cuts at: 1556 2616 2709 3765 1556
Size: 1060 93 1056 2782
Fragments arranged by size:
2782 1060 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1080 1503 1875 2203 2284 2619 4763 1080
Size: 423 372 328 81 335 2144 1308
Fragments arranged by size:
2144 1308 423 372 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 225 3358 225
Size: 3133 1858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 706 909 1299 352
Size: 354 203 390 4044
Fragments arranged by size:
4044 390 354 203
Bpu10I CC'TnA_GC
Cuts at: 159 159
Size: 4991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3046 3538 3836 2167
Size: 879 492 298 3322
Fragments arranged by size:
3322 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1819 4695 1819
Size: 2876 2115
BsaAI yAC'GTr
Cuts at: 2344 2344
Size: 4991
BsaBI GATnn'nnATC
Cuts at: 6 364 2505 4542 6
Size: 358 2141 2037 455
Fragments arranged by size:
2141 2037 455 358
BsaHI Gr'CG_yC
Cuts at: 2422 2670 3372 4868 2422
Size: 248 702 1496 2545
Fragments arranged by size:
2545 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1178 2180 2701 3379 3653 3800 4211 1178
Size: 1002 521 678 274 147 411 1958
Fragments arranged by size:
1958 1002 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 147 241 142
Size: 5 94 4892
Fragments arranged by size:
4892 94 5
BseYI C'CCAG_C
Cuts at: 2416 3174 3751 4247 2416
Size: 758 577 496 3160
Fragments arranged by size:
3160 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4991
BsiEI CG_ry'CG
Cuts at: 104 2533 2579 3787 104
Size: 2429 46 1208 1308
Fragments arranged by size:
2429 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 971 1629 2005 2783 2973 3765 275
Size: 696 658 376 778 190 792 1501
Fragments arranged by size:
1501 792 778 696 658 376 190
Bsp1286I G_dGCh'C
Cuts at: 265 275 971 1556 1629 2005 2616 2709 2783
Size: 10 696 585 73 376 611 93 74
Cuts at: 2783 2973 3035 3765 265
Size: 190 62 730 1491
Fragments arranged by size:
1491 730 696 611 585 376 190 93
74 73 62 10
BspEI T'CCGG_A
Cuts at: 4211 4211
Size: 4991
BspHI T'CATG_A
Cuts at: 1142 4167 1142
Size: 3025 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 989 2041 2255 2557 2938 989
Size: 1052 214 302 381 3042
Fragments arranged by size:
3042 1052 381 302 214
BsrBI CCG'CTC
Cuts at: 50 204 336 3283 3337 4821 4986 50
Size: 154 132 2947 54 1484 165 55
Fragments arranged by size:
2947 1484 165 154 132 55 54
BsrDI GCAATG_nn'
Cuts at: 1061 1923 2903 1061
Size: 862 980 3149
Fragments arranged by size:
3149 980 862
BsrFI r'CCGG_y
Cuts at: 231 2989 3170 3379 231
Size: 2758 181 209 1843
Fragments arranged by size:
2758 1843 209 181
BsrGI T'GTAC_A
Cuts at: 432 1694 432
Size: 1262 3729
BssHII G'CGCG_C
Cuts at: 115 187 115
Size: 72 4919
BssSI C'ACGA_G
Cuts at: 274 3262 3620 274
Size: 2988 358 1645
Fragments arranged by size:
2988 1645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 309 2368 309
Size: 2059 2932
BstBI TT'CG_AA
Cuts at: 564 3352 564
Size: 2788 2203
BstXI CCAn_nnnn'nTGG
Cuts at: 512 512
Size: 4991
BstYI r'GATC_y
Cuts at: 1 1302 2016 2483 2506 2841 3087 4099 4185
Size: 1301 714 467 23 335 246 1012 86
Cuts at: 4185 4197 4543 1
Size: 12 346 449
Fragments arranged by size:
1301 1012 714 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 731 835 731
Size: 104 4887
BtgI C'CryG_G
Cuts at: 107 266 806 107
Size: 159 540 4292
Fragments arranged by size:
4292 540 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2941 3122 3285 2941
Size: 181 163 4647
Fragments arranged by size:
4647 181 163
BtsI GCAGTG_nn'
Cuts at: 1009 1556 2234 1009
Size: 547 678 3766
Fragments arranged by size:
3766 678 547
DraI TTT'AAA
Cuts at: 1990 1990
Size: 4991
DraIII CAC_nnn'GTG
Cuts at: 390 453 390
Size: 63 4928
DrdI GACnn_nn'nnGTC
Cuts at: 910 2697 3555 910
Size: 1787 858 2346
Fragments arranged by size:
2346 1787 858
EaeI y'GGCC_r
Cuts at: 362 514 1856 2576 2750 3141 3168 4513 362
Size: 152 1342 720 174 391 27 1345 840
Fragments arranged by size:
1345 1342 840 720 391 174 152 27
EagI C'GGCC_G
Cuts at: 2576 2576
Size: 4991
EarI CTCTTCn'nnn_
Cuts at: 775 1513 3014 3224 775
Size: 738 1501 210 2542
Fragments arranged by size:
2542 1501 738 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3199 3509 3655 4801 3199
Size: 310 146 1146 3389
Fragments arranged by size:
3389 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2003 2003
Size: 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 706 909 1015 1285 1299 2816 3248 3995
Size: 354 203 106 270 14 1517 432 747
Cuts at: 3995 4263 352
Size: 268 1080
Fragments arranged by size:
1517 1080 747 432 354 270 268 203
106 14
EcoNI CCTnn'n_nnAGG
Cuts at: 838 838
Size: 4991
EcoO109I rG'GnC_Cy
Cuts at: 696 749 1743 1935 2130 696
Size: 53 994 192 195 3557
Fragments arranged by size:
3557 994 195 192 53
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4991
FspI TGC'GCA
Cuts at: 1189 2772 1189
Size: 1583 3408
HaeII r_GCGC'y
Cuts at: 2425 2673 3695 4674 2425
Size: 248 1022 979 2742
Fragments arranged by size:
2742 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 429 461 627 659 1050 1082 2833 2863 2865
Size: 32 166 32 391 32 1751 30 2
Cuts at: 2865 2895 429
Size: 30 2525
Fragments arranged by size:
2525 1751 391 166 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 612 2030 612
Size: 1418 3573
KasI G'GCGC_C
Cuts at: 2421 2669 2421
Size: 248 4743
KpnI G_GTAC'C
Cuts at: 1812 2011 1812
Size: 199 4792
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1620 3662 3846 1620
Size: 2042 184 2765
Fragments arranged by size:
2765 2042 184
MscI TGG'CCA
Cuts at: 364 516 1858 2752 364
Size: 152 1342 894 2603
Fragments arranged by size:
2603 1342 894 152
MslI CAynn'nnrTG
Cuts at: 946 1039 2980 3107 946
Size: 93 1941 127 2830
Fragments arranged by size:
2830 1941 127 93
MspA1I CmG'CkG
Cuts at: 1263 1488 2102 2416 2776 3789 4034 1263
Size: 225 614 314 360 1013 245 2220
Fragments arranged by size:
2220 1013 614 360 314 245 225
NaeI GCC'GGC
Cuts at: 3172 3172
Size: 4991
NarI GG'CG_CC
Cuts at: 2422 2670 2422
Size: 248 4743
NcoI C'CATG_G
Cuts at: 107 806 107
Size: 699 4292
NgoMIV G'CCGG_C
Cuts at: 3170 3170
Size: 4991
NspI r_CATG'y
Cuts at: 1882 2044 2544 3451 1882
Size: 162 500 907 3422
Fragments arranged by size:
3422 907 500 162
PasI CC'CwG_GG
Cuts at: 1599 1599
Size: 4991
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 1838 1865 1838
Size: 27 4964
PfoI T'CCnGG_A
Cuts at: 242 1891 242
Size: 1649 3342
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1757 1789 4130 4162 1757
Size: 32 2341 32 2586
Fragments arranged by size:
2586 2341 32 32
PpuMI rG'GwC_Cy
Cuts at: 749 1743 749
Size: 994 3997
PstI C_TGCA'G
Cuts at: 260 1016 1764 2038 2723 260
Size: 756 748 274 685 2528
Fragments arranged by size:
2528 756 748 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 1263 1488 2416 2776 1263
Size: 225 928 360 3478
Fragments arranged by size:
3478 928 360 225
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 2005 2005
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 4508 4508
Size: 4991
SexAI A'CCwGG_T
Cuts at: 1568 1568
Size: 4991
SfcI C'TryA_G
Cuts at: 33 256 1012 1760 1911 2034 2719 3712 3903
Size: 223 756 748 151 123 685 993 191
Cuts at: 3903 33
Size: 1121
Fragments arranged by size:
1121 993 756 748 685 223 191 151
123
SfoI GGC'GCC
Cuts at: 2423 2671 2423
Size: 248 4743
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 2013 2013
Size: 4991
SmlI C'TyrA_G
Cuts at: 2146 3061 3553 3815 2146
Size: 915 492 262 3322
Fragments arranged by size:
3322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 2044 2544 2044
Size: 500 4491
SspI AAT'ATT
Cuts at: 410 1278 410
Size: 868 4123
StuI AGG'CCT
Cuts at: 465 847 1320 465
Size: 382 473 4136
Fragments arranged by size:
4136 473 382
StyI C'CwwG_G
Cuts at: 107 806 2125 2479 107
Size: 699 1319 354 2619
Fragments arranged by size:
2619 1319 699 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2574 2707 3166 3375 2574
Size: 133 459 209 4190
Fragments arranged by size:
4190 459 209 133
TatI w'GTAC_w
Cuts at: 432 1694 1718 4506 432
Size: 1262 24 2788 917
Fragments arranged by size:
2788 1262 917 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 4959
Tth111I GACn'n_nGTC
Cuts at: 2788 2788
Size: 4991
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 357 2486 273
Size: 84 2129 2778
Fragments arranged by size:
2778 2129 84
XmaI C'CCGG_G
Cuts at: 2011 2011
Size: 4991
XmnI GAAnn'nnTTC
Cuts at: 1753 1753
Size: 4991
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI