Restriction map for
pF1KA0589
(Circular) (Six-base) MAPSORT of: pF1KA0589.seq Check: 4230 from: 1 to: 5117
pF1KA0589 5117 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1830 1983 1830
Size: 153 4964
AatII G_ACGT'C
Cuts at: 1738 1738
Size: 5117
AccI GT'mk_AC
Cuts at: 2155 2155
Size: 5117
Acc65I G'GTAC_C
Cuts at: 120 2133 120
Size: 2013 3104
AclI AA'CG_TT
Cuts at: 4957 4957
Size: 5117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 975 1477 2942 3374 4121 4389 364
Size: 611 502 1465 432 747 268 1092
Fragments arranged by size:
1465 1092 747 611 502 432 268
AfeI AGC'GCT
Cuts at: 141 1408 141
Size: 1267 3850
AflIII A'CryG_T
Cuts at: 2469 3573 4550 2469
Size: 1104 977 3036
Fragments arranged by size:
3036 1104 977
AgeI A'CCGG_T
Cuts at: 3505 3505
Size: 5117
AhdI GACnn_n'nnGTC
Cuts at: 1374 1374
Size: 5117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 679 711 679
Size: 32 5085
AlwNI CAG_nnn'CTG
Cuts at: 708 2422 3989 708
Size: 1714 1567 1836
Fragments arranged by size:
1836 1714 1567
ApaI G_GGCC'C
Cuts at: 262 1520 1590 262
Size: 1258 70 3789
Fragments arranged by size:
3789 1258 70
ApaLI G'TGCA_C
Cuts at: 1075 3887 1075
Size: 2812 2305
ApoI r'AATT_y
Cuts at: 2121 4470 4726 2121
Size: 2349 256 2512
Fragments arranged by size:
2512 2349 256
AscI GG'CGCG_CC
Cuts at: 891 891
Size: 5117
AseI AT'TA_AT
Cuts at: 20 20
Size: 5117
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
AvaI C'yCGr_G
Cuts at: 615 961 2137 615
Size: 346 1176 3595
Fragments arranged by size:
3595 1176 346
BamHI G'GATC_C
Cuts at: 554 1244 2142 554
Size: 690 898 3529
Fragments arranged by size:
3529 898 690
BanI G'GyrC_C
Cuts at: 120 349 619 1117 1198 1915 2133 2547 2795
Size: 229 270 498 81 717 218 414 248
Cuts at: 2795 2830 120
Size: 35 2407
Fragments arranged by size:
2407 717 498 414 270 248 229 218
81 35
BanII G_rGCy'C
Cuts at: 262 394 548 877 971 1520 1590 2131 3161
Size: 132 154 329 94 549 70 541 1030
Cuts at: 3161 262
Size: 2218
Fragments arranged by size:
2218 1030 549 541 329 154 132 94
70
BbeI G_GCGC'C
Cuts at: 623 1121 1202 2551 2799 623
Size: 498 81 1349 248 2941
Fragments arranged by size:
2941 1349 498 248 81
BbsI GAAGACnn'nnnn_
Cuts at: 331 331
Size: 5117
BbvCI CC'TCA_GC
Cuts at: 143 143
Size: 5117
BciVI GTATCCnnnnn_n'
Cuts at: 3015 3776 5117 3015
Size: 761 1341 3015
Fragments arranged by size:
3015 1341 761
BclI T'GATC_A
Cuts at: 2637 2637
Size: 5117
BglI GCCn_nnn'nGGC
Cuts at: 966 1534 1951 1984 2086 966
Size: 568 417 33 102 3997
Fragments arranged by size:
3997 568 417 102 33
BglII A'GATC_T
Cuts at: 2632 4669 2632
Size: 2037 3080
BlpI GC'TnA_GC
Cuts at: 2229 2229
Size: 5117
Bme1580I G_kGCm'C
Cuts at: 161 262 1079 1520 1590 2742 2835 3891 161
Size: 101 817 441 70 1152 93 1056 1387
Fragments arranged by size:
1387 1152 1056 817 441 101 93 70
BmgBI CAC'GTC
Cuts at: 411 411
Size: 5117
BmrI ACTGGGnnnn_n'
Cuts at: 1563 2329 2410 2745 4889 1563
Size: 766 81 335 2144 1791
Fragments arranged by size:
2144 1791 766 335 81
BmtI G_CTAG'C
Cuts at: 1501 1501
Size: 5117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3484 3484
Size: 5117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1354 1627 136
Size: 1218 273 3626
Fragments arranged by size:
3626 1218 273
Bpu10I CC'TnA_GC
Cuts at: 143 388 1715 143
Size: 245 1327 3545
Fragments arranged by size:
3545 1327 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 853 2293 3172 3664 3962 841
Size: 12 1440 879 492 298 1996
Fragments arranged by size:
1996 1440 879 492 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 4821 4821
Size: 5117
BsaAI yAC'GTr
Cuts at: 432 2470 432
Size: 2038 3079
BsaBI GATnn'nnATC
Cuts at: 6 2631 4668 6
Size: 2625 2037 455
Fragments arranged by size:
2625 2037 455
BsaHI Gr'CG_yC
Cuts at: 620 980 1118 1199 1735 2548 2796 3498 4994
Size: 360 138 81 536 813 248 702 1496
Cuts at: 4994 620
Size: 743
Fragments arranged by size:
1496 813 743 702 536 360 248 138
81
BsaWI w'CCGG_w
Cuts at: 123 2306 2827 3505 3779 3926 4337 123
Size: 2183 521 678 274 147 411 903
Fragments arranged by size:
2183 903 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 376 406 562 592 679 709 1555
Size: 30 189 30 156 30 87 30 846
Cuts at: 1555 1585 1837 1867 157
Size: 30 252 30 3407
Fragments arranged by size:
3407 846 252 189 156 87 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 1440 1576 1813 1897 1978 2011 2055 324
Size: 1116 136 237 84 81 33 44 3386
Fragments arranged by size:
3386 1116 237 136 84 81 44 33
BseYI C'CCAG_C
Cuts at: 360 454 1068 1390 1512 2045 2542 3300 3877
Size: 94 614 322 122 533 497 758 577
Cuts at: 3877 4373 360
Size: 496 1104
Fragments arranged by size:
1104 758 614 577 533 497 496 322
122 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1267 1623 1858 1875 805
Size: 462 356 235 17 4047
Fragments arranged by size:
4047 462 356 235 17
BsiEI CG_ry'CG
Cuts at: 104 1263 1913 2659 2705 3913 104
Size: 1159 650 746 46 1208 1308
Fragments arranged by size:
1308 1208 1159 746 650 46
BsiHKAI G_wGCw'C
Cuts at: 394 416 548 1079 1129 1340 2131 2909 3099
Size: 22 132 531 50 211 791 778 190
Cuts at: 3099 3891 394
Size: 792 1620
Fragments arranged by size:
1620 792 791 778 531 211 190 132
50 22
BsmBI CGTCTCn'nnnn_
Cuts at: 1726 1726
Size: 5117
Bsp1286I G_dGCh'C
Cuts at: 161 262 394 416 548 877 971 1079 1129
Size: 101 132 22 132 329 94 108 50
Cuts at: 1129 1340 1520 1590 2131 2742 2835 2909 3099
Size: 211 180 70 541 611 93 74 190
Cuts at: 3099 3161 3891 161
Size: 62 730 1387
Fragments arranged by size:
1387 730 611 541 329 211 190 180
132 132 108 101 94 93 74 70
62 50 22
BspEI T'CCGG_A
Cuts at: 4337 4337
Size: 5117
BspHI T'CATG_A
Cuts at: 818 1421 4293 818
Size: 603 2872 1642
Fragments arranged by size:
2872 1642 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 1586 1830 1983 2167 2381 2683 3064 190
Size: 1396 244 153 184 214 302 381 2243
Fragments arranged by size:
2243 1396 381 302 244 214 184 153
BsrBI CCG'CTC
Cuts at: 50 188 1104 1272 1557 1719 2061 3409 3463
Size: 138 916 168 285 162 342 1348 54
Cuts at: 3463 4947 5112 50
Size: 1484 165 55
Fragments arranged by size:
1484 1348 916 342 285 168 165 162
138 55 54
BsrDI GCAATG_nn'
Cuts at: 594 1676 3029 594
Size: 1082 1353 2682
Fragments arranged by size:
2682 1353 1082
BsrFI r'CCGG_y
Cuts at: 1619 1912 3115 3296 3505 1619
Size: 293 1203 181 209 3231
Fragments arranged by size:
3231 1203 293 209 181
BssHII G'CGCG_C
Cuts at: 891 1204 891
Size: 313 4804
BssSI C'ACGA_G
Cuts at: 1093 3388 3746 1093
Size: 2295 358 2464
Fragments arranged by size:
2464 2295 358
BstAPI GCAn_nnn'nTGC
Cuts at: 708 957 2494 708
Size: 249 1537 3331
Fragments arranged by size:
3331 1537 249
BstBI TT'CG_AA
Cuts at: 1655 3478 1655
Size: 1823 3294
BstXI CCAn_nnnn'nTGG
Cuts at: 114 360 1179 114
Size: 246 819 4052
Fragments arranged by size:
4052 819 246
BstYI r'GATC_y
Cuts at: 1 554 1244 1544 1818 2142 2609 2632 2967
Size: 553 690 300 274 324 467 23 335
Cuts at: 2967 3213 4225 4311 4323 4669 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 690 553 467 449 346 335 324
300 274 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 267 377 745 1172 1178 1263 1747 1808
Size: 160 110 368 427 6 85 484 61
Cuts at: 1808 107
Size: 3416
Fragments arranged by size:
3416 484 427 368 160 110 85 61
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3067 3248 3411 3067
Size: 181 163 4773
Fragments arranged by size:
4773 181 163
BtsI GCAGTG_nn'
Cuts at: 2360 2360
Size: 5117
DraI TTT'AAA
Cuts at: 2116 2116
Size: 5117
DrdI GACnn_nn'nnGTC
Cuts at: 1027 2823 3681 1027
Size: 1796 858 2463
Fragments arranged by size:
2463 1796 858
EaeI y'GGCC_r
Cuts at: 264 559 1175 1260 1535 1910 2702 2876 3267
Size: 295 616 85 275 375 792 174 391
Cuts at: 3267 3294 4639 264
Size: 27 1345 742
Fragments arranged by size:
1345 792 742 616 391 375 295 275
174 85 27
EagI C'GGCC_G
Cuts at: 1260 1910 2702 1260
Size: 650 792 3675
Fragments arranged by size:
3675 792 650
EarI CTCTTCn'nnn_
Cuts at: 640 910 1808 3140 3350 640
Size: 270 898 1332 210 2407
Fragments arranged by size:
2407 1332 898 270 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 1502 3325 3635 3781 4927 148
Size: 1354 1823 310 146 1146 338
Fragments arranged by size:
1823 1354 1146 338 310 146
EcoICRI GAG'CTC
Cuts at: 392 546 2129 392
Size: 154 1583 3380
Fragments arranged by size:
3380 1583 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 364 975 1354 1477 1627 2942 3374 4121
Size: 228 611 379 123 150 1315 432 747
Cuts at: 4121 4389 136
Size: 268 864
Fragments arranged by size:
1315 864 747 611 432 379 268 228
150 123
EcoO109I rG'GnC_Cy
Cuts at: 259 936 1350 1516 1586 2079 2256 259
Size: 677 414 166 70 493 177 3120
Fragments arranged by size:
3120 677 493 414 177 166 70
EcoRI G'AATT_C
Cuts at: 2121 2121
Size: 5117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 5117
FseI GG_CCGG'CC
Cuts at: 1623 1623
Size: 5117
FspI TGC'GCA
Cuts at: 2898 2898
Size: 5117
HaeII r_GCGC'y
Cuts at: 143 623 998 1121 1165 1202 1410 2075 2551
Size: 480 375 123 44 37 208 665 476
Cuts at: 2551 2799 3821 4800 143
Size: 248 1022 979 460
Fragments arranged by size:
1022 979 665 480 476 460 375 248
208 123 44 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 1360 1392 2959 2989 2991 3021 223
Size: 32 1105 32 1567 30 2 30 2319
Fragments arranged by size:
2319 1567 1105 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2156 2156
Size: 5117
KasI G'GCGC_C
Cuts at: 619 1117 1198 2547 2795 619
Size: 498 81 1349 248 2941
Fragments arranged by size:
2941 1349 498 248 81
KpnI G_GTAC'C
Cuts at: 124 2137 124
Size: 2013 3104
MluI A'CGCG_T
Cuts at: 4550 4550
Size: 5117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 1718 2117 3788 3972 635
Size: 1083 399 1671 184 1780
Fragments arranged by size:
1780 1671 1083 399 184
MscI TGG'CCA
Cuts at: 266 2878 266
Size: 2612 2505
MslI CAynn'nnrTG
Cuts at: 290 1177 3106 3233 290
Size: 887 1929 127 2174
Fragments arranged by size:
2174 1929 887 127
MspA1I CmG'CkG
Cuts at: 360 597 747 1017 1252 1265 1512 1525 1749
Size: 237 150 270 235 13 247 13 224
Cuts at: 1749 1810 2228 2542 2902 3915 4160 360
Size: 61 418 314 360 1013 245 1317
Fragments arranged by size:
1317 1013 418 360 314 270 247 245
237 235 224 150 61 13 13
NaeI GCC'GGC
Cuts at: 1621 3298 1621
Size: 1677 3440
NarI GG'CG_CC
Cuts at: 620 1118 1199 2548 2796 620
Size: 498 81 1349 248 2941
Fragments arranged by size:
2941 1349 498 248 81
NcoI C'CATG_G
Cuts at: 107 267 1178 107
Size: 160 911 4046
Fragments arranged by size:
4046 911 160
NgoMIV G'CCGG_C
Cuts at: 1619 3296 1619
Size: 1677 3440
NheI G'CTAG_C
Cuts at: 1497 1497
Size: 5117
NspI r_CATG'y
Cuts at: 960 2170 2670 3577 960
Size: 1210 500 907 2500
Fragments arranged by size:
2500 1210 907 500
PciI A'CATG_T
Cuts at: 3573 3573
Size: 5117
PfoI T'CCnGG_A
Cuts at: 491 539 1031 491
Size: 48 492 4577
Fragments arranged by size:
4577 492 48
PmeI GTTT'AAAC
Cuts at: 2116 2116
Size: 5117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 679 711 4256 4288 679
Size: 32 3545 32 1508
Fragments arranged by size:
3545 1508 32 32
PpuMI rG'GwC_Cy
Cuts at: 936 936
Size: 5117
PspOMI G'GGCC_C
Cuts at: 258 1516 1586 258
Size: 1258 70 3789
Fragments arranged by size:
3789 1258 70
PstI C_TGCA'G
Cuts at: 704 1016 1310 1826 2164 2849 704
Size: 312 294 516 338 685 2972
Fragments arranged by size:
2972 685 516 338 312 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5117
PvuII CAG'CTG
Cuts at: 360 2542 2902 360
Size: 2182 360 2575
Fragments arranged by size:
2575 2182 360
RsrII CG'GwC_CG
Cuts at: 3312 3312
Size: 5117
SacI G_AGCT'C
Cuts at: 394 548 2131 394
Size: 154 1583 3380
Fragments arranged by size:
3380 1583 154
SacII CC_GC'GG
Cuts at: 748 1266 1750 1811 748
Size: 518 484 61 4054
Fragments arranged by size:
4054 518 484 61
SalI G'TCGA_C
Cuts at: 2154 2154
Size: 5117
SapI GCTCTTCn'nnn_
Cuts at: 910 3140 3350 910
Size: 2230 210 2677
Fragments arranged by size:
2677 2230 210
SbfI CC_TGCA'GG
Cuts at: 2164 2164
Size: 5117
ScaI AGT'ACT
Cuts at: 4634 4634
Size: 5117
SfcI C'TryA_G
Cuts at: 33 700 1012 1056 1306 1314 1667 1822 2067
Size: 667 312 44 250 8 353 155 245
Cuts at: 2067 2160 2845 3838 4029 33
Size: 93 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 353 312 250 245
191 155 93 44 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 2086 2086
Size: 5117
SfoI GGC'GCC
Cuts at: 621 1119 1200 2549 2797 621
Size: 498 81 1349 248 2941
Fragments arranged by size:
2941 1349 498 248 81
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
SmaI CCC'GGG
Cuts at: 617 2139 617
Size: 1522 3595
SmlI C'TyrA_G
Cuts at: 856 868 2272 3187 3679 3941 856
Size: 12 1404 915 492 262 2032
Fragments arranged by size:
2032 1404 915 492 262 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5117
SphI G_CATG'C
Cuts at: 2170 2670 2170
Size: 500 4617
StuI AGG'CCT
Cuts at: 1954 1987 1954
Size: 33 5084
StyI C'CwwG_G
Cuts at: 107 267 1178 2251 2605 107
Size: 160 911 1073 354 2619
Fragments arranged by size:
2619 1073 911 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2037 2700 2833 3292 3501 2037
Size: 663 133 459 209 3653
Fragments arranged by size:
3653 663 459 209 133
TatI w'GTAC_w
Cuts at: 575 776 2051 4632 575
Size: 201 1275 2581 1060
Fragments arranged by size:
2581 1275 1060 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 376 408 1837 1869 4634 4666 157
Size: 32 187 32 1429 32 2765 32 608
Fragments arranged by size:
2765 1429 608 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1689 2914 1689
Size: 1225 3892
XbaI T'CTAG_A
Cuts at: 2148 2148
Size: 5117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2612 2612
Size: 5117
XmaI C'CCGG_G
Cuts at: 615 2137 615
Size: 1522 3595
XmnI GAAnn'nnTTC
Cuts at: 919 919
Size: 5117
ZraI GAC'GTC
Cuts at: 1736 1736
Size: 5117
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AleI AlfI AvrII BaeI BcgI BfrBI BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI