Restriction map for
pF1KA0594
(Circular) (Six-base) MAPSORT of: pF1KA0594.seq Check: 1830 from: 1 to: 6416
pF1KA0594 6416 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 133 133
Size: 6416
AccI GT'mk_AC
Cuts at: 3320 3454 3320
Size: 134 6282
Acc65I G'GTAC_C
Cuts at: 3432 3432
Size: 6416
AclI AA'CG_TT
Cuts at: 6256 6256
Size: 6416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1279 2747 3112 4241 4673 5420 5688 1156
Size: 123 1468 365 1129 432 747 268 1884
Fragments arranged by size:
1884 1468 1129 747 432 365 268 123
AflII C'TTAA_G
Cuts at: 759 1632 759
Size: 873 5543
AflIII A'CryG_T
Cuts at: 1027 1493 3768 4872 5849 1027
Size: 466 2275 1104 977 1594
Fragments arranged by size:
2275 1594 1104 977 466
AgeI A'CCGG_T
Cuts at: 4804 4804
Size: 6416
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 6416
AlwNI CAG_nnn'CTG
Cuts at: 3721 5288 3721
Size: 1567 4849
ApaLI G'TGCA_C
Cuts at: 5186 5186
Size: 6416
ApoI r'AATT_y
Cuts at: 207 664 1534 2090 2932 3017 3034 3357 3420
Size: 457 870 556 842 85 17 323 63
Cuts at: 3420 5769 6025 207
Size: 2349 256 598
Fragments arranged by size:
2349 870 842 598 556 457 323 256
85 63 17
AseI AT'TA_AT
Cuts at: 20 2924 20
Size: 2904 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
AvaI C'yCGr_G
Cuts at: 168 192 781 3436 168
Size: 24 589 2655 3148
Fragments arranged by size:
3148 2655 589 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2618 2651 2618
Size: 33 6383
BamHI G'GATC_C
Cuts at: 3441 3441
Size: 6416
BanI G'GyrC_C
Cuts at: 3383 3432 3846 4094 4129 3383
Size: 49 414 248 35 5670
Fragments arranged by size:
5670 414 248 49 35
BanII G_rGCy'C
Cuts at: 165 1559 3430 4460 165
Size: 1394 1871 1030 2121
Fragments arranged by size:
2121 1871 1394 1030
BbeI G_GCGC'C
Cuts at: 3387 3850 4098 3387
Size: 463 248 5705
Fragments arranged by size:
5705 463 248
BbsI GAAGACnn'nnnn_
Cuts at: 133 2070 133
Size: 1937 4479
BciVI GTATCCnnnnn_n'
Cuts at: 2271 4314 5075 6416 2271
Size: 2043 761 1341 2271
Fragments arranged by size:
2271 2043 1341 761
BclI T'GATC_A
Cuts at: 3936 3936
Size: 6416
BglII A'GATC_T
Cuts at: 1762 3931 5968 1762
Size: 2169 2037 2210
Fragments arranged by size:
2210 2169 2037
BlpI GC'TnA_GC
Cuts at: 3528 3528
Size: 6416
Bme1580I G_kGCm'C
Cuts at: 4041 4134 5190 4041
Size: 93 1056 5267
Fragments arranged by size:
5267 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3628 3709 4044 6188 3628
Size: 81 335 2144 3856
Fragments arranged by size:
3856 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1412 4783 1412
Size: 3371 3045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 2176
Size: 6416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 2600 3592 4471 4963 5261 2221
Size: 379 992 879 492 298 3376
Fragments arranged by size:
3376 992 879 492 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 167 778 6120 167
Size: 611 5342 463
Fragments arranged by size:
5342 611 463
BsaAI yAC'GTr
Cuts at: 1496 2137 3769 1496
Size: 641 1632 4143
Fragments arranged by size:
4143 1632 641
BsaBI GATnn'nnATC
Cuts at: 6 3930 5967 6
Size: 3924 2037 455
Fragments arranged by size:
3924 2037 455
BsaHI Gr'CG_yC
Cuts at: 130 3384 3847 4095 4797 6293 130
Size: 3254 463 248 702 1496 253
Fragments arranged by size:
3254 1496 702 463 253 248
BsaWI w'CCGG_w
Cuts at: 3605 4126 4804 5078 5225 5636 3605
Size: 521 678 274 147 411 4385
Fragments arranged by size:
4385 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 120 150 1212 1242 3257 3287 120
Size: 30 1062 30 2015 30 3249
Fragments arranged by size:
3249 2015 1062 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 1233 2652 3059 682
Size: 551 1419 407 4039
Fragments arranged by size:
4039 1419 551 407
BseYI C'CCAG_C
Cuts at: 146 3841 4599 5176 5672 146
Size: 3695 758 577 496 890
Fragments arranged by size:
3695 890 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 2618
Size: 6416
BsiEI CG_ry'CG
Cuts at: 104 3958 4004 5212 104
Size: 3854 46 1208 1308
Fragments arranged by size:
3854 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 165 2162 3430 4208 4398 5190 165
Size: 1997 1268 778 190 792 1391
Fragments arranged by size:
1997 1391 1268 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1323 1323
Size: 6416
Bsp1286I G_dGCh'C
Cuts at: 165 1559 2162 3430 4041 4134 4208 4398 4460
Size: 1394 603 1268 611 93 74 190 62
Cuts at: 4460 5190 165
Size: 730 1391
Fragments arranged by size:
1394 1391 1268 730 611 603 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5636 5636
Size: 6416
BspHI T'CATG_A
Cuts at: 1866 5592 1866
Size: 3726 2690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 417 3466 3680 3982 4363 417
Size: 3049 214 302 381 2470
Fragments arranged by size:
3049 2470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4708 4762 6246 6411 50
Size: 4658 54 1484 165 55
Fragments arranged by size:
4658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4328 4328
Size: 6416
BsrFI r'CCGG_y
Cuts at: 4414 4595 4804 4414
Size: 181 209 6026
Fragments arranged by size:
6026 209 181
BssSI C'ACGA_G
Cuts at: 4687 5045 4687
Size: 358 6058
BstAPI GCAn_nnn'nTGC
Cuts at: 3793 3793
Size: 6416
BstBI TT'CG_AA
Cuts at: 3021 3039 4777 3021
Size: 18 1738 4660
Fragments arranged by size:
4660 1738 18
BstXI CCAn_nnnn'nTGG
Cuts at: 3353 3353
Size: 6416
BstYI r'GATC_y
Cuts at: 1 1762 3441 3908 3931 4266 4512 5524 5610
Size: 1761 1679 467 23 335 246 1012 86
Cuts at: 5610 5622 5968 1
Size: 12 346 449
Fragments arranged by size:
1761 1679 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 647 647
Size: 6416
BtgI C'CryG_G
Cuts at: 107 919 1274 2672 107
Size: 812 355 1398 3851
Fragments arranged by size:
3851 1398 812 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4366 4547 4710 4366
Size: 181 163 6072
Fragments arranged by size:
6072 181 163
BtsI GCAGTG_nn'
Cuts at: 2117 2965 3659 2117
Size: 848 694 4874
Fragments arranged by size:
4874 848 694
DraI TTT'AAA
Cuts at: 1539 2261 2903 3362 3415 1539
Size: 722 642 459 53 4540
Fragments arranged by size:
4540 722 642 459 53
DrdI GACnn_nn'nnGTC
Cuts at: 4122 4980 4122
Size: 858 5558
EaeI y'GGCC_r
Cuts at: 2474 4001 4175 4566 4593 5938 2474
Size: 1527 174 391 27 1345 2952
Fragments arranged by size:
2952 1527 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4001 4001
Size: 6416
EarI CTCTTCn'nnn_
Cuts at: 445 587 983 1196 1377 2288 2294 2615 4439
Size: 142 396 213 181 911 6 321 1824
Cuts at: 4439 4649 445
Size: 210 2212
Fragments arranged by size:
2212 1824 911 396 321 213 210 181
142 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4624 4934 5080 6226 4624
Size: 310 146 1146 4814
Fragments arranged by size:
4814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 163 3428 163
Size: 3265 3151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1279 2176 2747 3112 4241 4673 5420 5688
Size: 123 897 571 365 1129 432 747 268
Cuts at: 5688 1156
Size: 1884
Fragments arranged by size:
1884 1129 897 747 571 432 365 268
123
EcoNI CCTnn'n_nnAGG
Cuts at: 1681 1681
Size: 6416
EcoO109I rG'GnC_Cy
Cuts at: 189 3555 189
Size: 3366 3050
EcoRI G'AATT_C
Cuts at: 207 3017 3420 207
Size: 2810 403 3203
Fragments arranged by size:
3203 2810 403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 4435 2368
Size: 2067 4349
FspI TGC'GCA
Cuts at: 4197 4197
Size: 6416
HaeII r_GCGC'y
Cuts at: 3387 3850 4098 5120 6099 3387
Size: 463 248 1022 979 3704
Fragments arranged by size:
3704 1022 979 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 166 198 2598 2630 4258 4288 4290
Size: 32 14 32 2400 32 1628 30 2
Cuts at: 4290 4320 120
Size: 30 2216
Fragments arranged by size:
2400 2216 1628 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 134 2816 3455 134
Size: 2682 639 3095
Fragments arranged by size:
3095 2682 639
HpaI GTT'AAC
Cuts at: 2816 2816
Size: 6416
KasI G'GCGC_C
Cuts at: 3383 3846 4094 3383
Size: 463 248 5705
Fragments arranged by size:
5705 463 248
KpnI G_GTAC'C
Cuts at: 3436 3436
Size: 6416
MfeI C'AATT_G
Cuts at: 713 2771 713
Size: 2058 4358
MluI A'CGCG_T
Cuts at: 5849 5849
Size: 6416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 324 639 5087 5271 164
Size: 160 315 4448 184 1309
Fragments arranged by size:
4448 1309 315 184 160
MscI TGG'CCA
Cuts at: 4177 4177
Size: 6416
MslI CAynn'nnrTG
Cuts at: 277 1865 3080 4405 4532 277
Size: 1588 1215 1325 127 2161
Fragments arranged by size:
2161 1588 1325 1215 127
MspA1I CmG'CkG
Cuts at: 222 228 3527 3841 4201 5214 5459 222
Size: 6 3299 314 360 1013 245 1179
Fragments arranged by size:
3299 1179 1013 360 314 245 6
NaeI GCC'GGC
Cuts at: 4597 4597
Size: 6416
NarI GG'CG_CC
Cuts at: 3384 3847 4095 3384
Size: 463 248 5705
Fragments arranged by size:
5705 463 248
NcoI C'CATG_G
Cuts at: 107 919 2672 107
Size: 812 1753 3851
Fragments arranged by size:
3851 1753 812
NdeI CA'TA_TG
Cuts at: 297 1740 3335 297
Size: 1443 1595 3378
Fragments arranged by size:
3378 1595 1443
NgoMIV G'CCGG_C
Cuts at: 4595 4595
Size: 6416
NspI r_CATG'y
Cuts at: 1031 3469 3969 4876 1031
Size: 2438 500 907 2571
Fragments arranged by size:
2571 2438 907 500
PciI A'CATG_T
Cuts at: 1027 4872 1027
Size: 3845 2571
PfoI T'CCnGG_A
Cuts at: 318 318
Size: 6416
PmeI GTTT'AAAC
Cuts at: 3415 3415
Size: 6416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 476 5555 5587 444
Size: 32 5079 32 1273
Fragments arranged by size:
5079 1273 32 32
PpuMI rG'GwC_Cy
Cuts at: 189 189
Size: 6416
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 953 985 1901 1933 953
Size: 32 916 32 5436
Fragments arranged by size:
5436 916 32 32
PstI C_TGCA'G
Cuts at: 3463 4148 3463
Size: 685 5731
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6416
PvuII CAG'CTG
Cuts at: 222 3841 4201 222
Size: 3619 360 2437
Fragments arranged by size:
3619 2437 360
RsrII CG'GwC_CG
Cuts at: 4611 4611
Size: 6416
SacI G_AGCT'C
Cuts at: 165 3430 165
Size: 3265 3151
SalI G'TCGA_C
Cuts at: 3453 3453
Size: 6416
SapI GCTCTTCn'nnn_
Cuts at: 1196 2294 4439 4649 1196
Size: 1098 2145 210 2963
Fragments arranged by size:
2963 2145 1098 210
SbfI CC_TGCA'GG
Cuts at: 3463 3463
Size: 6416
ScaI AGT'ACT
Cuts at: 3048 5933 3048
Size: 2885 3531
SfcI C'TryA_G
Cuts at: 33 814 1877 2432 3459 4144 5137 5328 33
Size: 781 1063 555 1027 685 993 191 1121
Fragments arranged by size:
1121 1063 1027 993 781 685 555 191
SfoI GGC'GCC
Cuts at: 3385 3848 4096 3385
Size: 463 248 5705
Fragments arranged by size:
5705 463 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6416
SmaI CCC'GGG
Cuts at: 3438 3438
Size: 6416
SmlI C'TyrA_G
Cuts at: 759 781 1632 2200 2615 3571 4486 4978 5240
Size: 22 851 568 415 956 915 492 262
Cuts at: 5240 759
Size: 1935
Fragments arranged by size:
1935 956 915 851 568 492 415 262
22
SpeI A'CTAG_T
Cuts at: 67 966 67
Size: 899 5517
SphI G_CATG'C
Cuts at: 3469 3969 3469
Size: 500 5916
SspI AAT'ATT
Cuts at: 615 615
Size: 6416
StyI C'CwwG_G
Cuts at: 107 390 919 2672 3550 3904 107
Size: 283 529 1753 878 354 2619
Fragments arranged by size:
2619 1753 878 529 354 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 912 992 1226 2584 3999 4132 4591 4800 912
Size: 80 234 1358 1415 133 459 209 2528
Fragments arranged by size:
2528 1415 1358 459 234 209 133 80
TatI w'GTAC_w
Cuts at: 2386 3046 5931 2386
Size: 660 2885 2871
Fragments arranged by size:
2885 2871 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 5933 5965 3257
Size: 32 2644 32 3708
Fragments arranged by size:
3708 2644 32 32
Tth111I GACn'n_nGTC
Cuts at: 4213 4213
Size: 6416
XbaI T'CTAG_A
Cuts at: 463 1398 3447 463
Size: 935 2049 3432
Fragments arranged by size:
3432 2049 935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3911 3911
Size: 6416
XhoI C'TCGA_G
Cuts at: 781 781
Size: 6416
XmaI C'CCGG_G
Cuts at: 3436 3436
Size: 6416
XmnI GAAnn'nnTTC
Cuts at: 938 3099 938
Size: 2161 4255
ZraI GAC'GTC
Cuts at: 131 131
Size: 6416
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI