Restriction map for
pF1KA0597
(Circular) (Six-base) MAPSORT of: pF1KA0597.seq Check: 7722 from: 1 to: 5843
pF1KA0597 5843 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2881 2881
Size: 5843
Acc65I G'GTAC_C
Cuts at: 2859 2859
Size: 5843
AclI AA'CG_TT
Cuts at: 5683 5683
Size: 5843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1687 1774 3668 4100 4847 5115 935
Size: 752 87 1894 432 747 268 1663
Fragments arranged by size:
1894 1663 752 747 432 268 87
AflIII A'CryG_T
Cuts at: 972 1077 3195 4299 5276 972
Size: 105 2118 1104 977 1539
Fragments arranged by size:
2118 1539 1104 977 105
AgeI A'CCGG_T
Cuts at: 4231 4231
Size: 5843
AhdI GACnn_n'nnGTC
Cuts at: 1453 1453
Size: 5843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 299 299
Size: 5843
AlwNI CAG_nnn'CTG
Cuts at: 1752 3148 4715 1752
Size: 1396 1567 2880
Fragments arranged by size:
2880 1567 1396
ApaLI G'TGCA_C
Cuts at: 561 4613 561
Size: 4052 1791
ApoI r'AATT_y
Cuts at: 519 1180 1473 1989 2847 5196 5452 519
Size: 661 293 516 858 2349 256 910
Fragments arranged by size:
2349 910 858 661 516 293 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
AvaI C'yCGr_G
Cuts at: 1687 2863 1687
Size: 1176 4667
BamHI G'GATC_C
Cuts at: 2868 2868
Size: 5843
BanI G'GyrC_C
Cuts at: 2859 3273 3521 3556 2859
Size: 414 248 35 5146
Fragments arranged by size:
5146 414 248 35
BanII G_rGCy'C
Cuts at: 481 870 2132 2318 2857 3887 481
Size: 389 1262 186 539 1030 2437
Fragments arranged by size:
2437 1262 1030 539 389 186
BbeI G_GCGC'C
Cuts at: 3277 3525 3277
Size: 248 5595
BbsI GAAGACnn'nnnn_
Cuts at: 131 969 1395 2013 2269 131
Size: 838 426 618 256 3705
Fragments arranged by size:
3705 838 618 426 256
BciVI GTATCCnnnnn_n'
Cuts at: 1783 3741 4502 5843 1783
Size: 1958 761 1341 1783
Fragments arranged by size:
1958 1783 1341 761
BclI T'GATC_A
Cuts at: 723 2255 3363 723
Size: 1532 1108 3203
Fragments arranged by size:
3203 1532 1108
BglI GCCn_nnn'nGGC
Cuts at: 321 321
Size: 5843
BglII A'GATC_T
Cuts at: 1189 3358 5395 1189
Size: 2169 2037 1637
Fragments arranged by size:
2169 2037 1637
BlpI GC'TnA_GC
Cuts at: 2955 2955
Size: 5843
Bme1580I G_kGCm'C
Cuts at: 565 2400 3468 3561 4617 565
Size: 1835 1068 93 1056 1791
Fragments arranged by size:
1835 1791 1068 1056 93
BmgBI CAC'GTC
Cuts at: 560 1080 560
Size: 520 5323
BmrI ACTGGGnnnn_n'
Cuts at: 1213 2403 3055 3136 3471 5615 1213
Size: 1190 652 81 335 2144 1441
Fragments arranged by size:
2144 1441 1190 652 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4210 4210
Size: 5843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 671 1340 1652 1927 2312 290
Size: 381 669 312 275 385 3821
Fragments arranged by size:
3821 669 385 381 312 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3019 3898 4390 4688 3019
Size: 879 492 298 4174
Fragments arranged by size:
4174 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1236 1817 2155 2252 5547 1236
Size: 581 338 97 3295 1532
Fragments arranged by size:
3295 1532 581 338 97
BsaAI yAC'GTr
Cuts at: 1606 3196 1606
Size: 1590 4253
BsaBI GATnn'nnATC
Cuts at: 6 1493 3357 5394 6
Size: 1487 1864 2037 455
Fragments arranged by size:
2037 1864 1487 455
BsaHI Gr'CG_yC
Cuts at: 3274 3522 4224 5720 3274
Size: 248 702 1496 3397
Fragments arranged by size:
3397 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2498 3032 3553 4231 4505 4652 5063 2498
Size: 534 521 678 274 147 411 3278
Fragments arranged by size:
3278 678 534 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1399 1429 1943 1973 1399
Size: 30 514 30 5269
Fragments arranged by size:
5269 514 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 817 820 832 2360 814
Size: 3 3 12 1528 4297
Fragments arranged by size:
4297 1528 12 3 3
BseYI C'CCAG_C
Cuts at: 3268 4026 4603 5099 3268
Size: 758 577 496 4012
Fragments arranged by size:
4012 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 725
Size: 5843
BsiEI CG_ry'CG
Cuts at: 104 3385 3431 4639 104
Size: 3281 46 1208 1308
Fragments arranged by size:
3281 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 481 565 624 2132 2857 3635 3825 4617 481
Size: 84 59 1508 725 778 190 792 1707
Fragments arranged by size:
1707 1508 792 778 725 190 84 59
BsmI GAATG_Cn'
Cuts at: 226 574 894 941 226
Size: 348 320 47 5128
Fragments arranged by size:
5128 348 320 47
Bsp1286I G_dGCh'C
Cuts at: 481 565 624 870 2132 2318 2400 2857 3468
Size: 84 59 246 1262 186 82 457 611
Cuts at: 3468 3561 3635 3825 3887 4617 481
Size: 93 74 190 62 730 1707
Fragments arranged by size:
1707 1262 730 611 457 246 190 186
93 84 82 74 62 59
BspEI T'CCGG_A
Cuts at: 2498 5063 2498
Size: 2565 3278
BspHI T'CATG_A
Cuts at: 2252 2258 5019 2252
Size: 6 2761 3076
Fragments arranged by size:
3076 2761 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2893 3107 3409 3790 2893
Size: 214 302 381 4946
Fragments arranged by size:
4946 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4135 4189 5673 5838 50
Size: 4085 54 1484 165 55
Fragments arranged by size:
4085 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 3755 2423
Size: 1332 4511
BsrFI r'CCGG_y
Cuts at: 3841 4022 4231 3841
Size: 181 209 5453
Fragments arranged by size:
5453 209 181
BssSI C'ACGA_G
Cuts at: 1740 2770 4114 4472 1740
Size: 1030 1344 358 3111
Fragments arranged by size:
3111 1344 1030 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3220 3220
Size: 5843
BstBI TT'CG_AA
Cuts at: 4204 4204
Size: 5843
BstYI r'GATC_y
Cuts at: 1 1189 1800 2868 3335 3358 3693 3939 4951
Size: 1188 611 1068 467 23 335 246 1012
Cuts at: 4951 5037 5049 5395 1
Size: 86 12 346 449
Fragments arranged by size:
1188 1068 1012 611 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2347 2207
Size: 140 5703
BtgI C'CryG_G
Cuts at: 107 116 612 2383 107
Size: 9 496 1771 3567
Fragments arranged by size:
3567 1771 496 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3793 3974 4137 3793
Size: 181 163 5499
Fragments arranged by size:
5499 181 163
BtsI GCAGTG_nn'
Cuts at: 368 761 1753 3086 368
Size: 393 992 1333 3125
Fragments arranged by size:
3125 1333 992 393
DraI TTT'AAA
Cuts at: 995 1988 2842 995
Size: 993 854 3996
Fragments arranged by size:
3996 993 854
DraIII CAC_nnn'GTG
Cuts at: 569 571 569
Size: 2 5841
DrdI GACnn_nn'nnGTC
Cuts at: 3549 4407 3549
Size: 858 4985
EaeI y'GGCC_r
Cuts at: 2082 3428 3602 3993 4020 5365 2082
Size: 1346 174 391 27 1345 2560
Fragments arranged by size:
2560 1346 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3428 3428
Size: 5843
EarI CTCTTCn'nnn_
Cuts at: 791 911 3866 4076 791
Size: 120 2955 210 2558
Fragments arranged by size:
2955 2558 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2664 2698 4051 4361 4507 5653 2664
Size: 34 1353 310 146 1146 2854
Fragments arranged by size:
2854 1353 1146 310 146 34
EcoICRI GAG'CTC
Cuts at: 479 2130 2855 479
Size: 1651 725 3467
Fragments arranged by size:
3467 1651 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 671 935 1340 1652 1687 1774 1927 2312
Size: 381 264 405 312 35 87 153 385
Cuts at: 2312 3668 4100 4847 5115 290
Size: 1356 432 747 268 1018
Fragments arranged by size:
1356 1018 747 432 405 385 381 312
268 264 153 87 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 2609
Size: 5843
EcoO109I rG'GnC_Cy
Cuts at: 1954 2446 2982 1954
Size: 492 536 4815
Fragments arranged by size:
4815 536 492
EcoRI G'AATT_C
Cuts at: 2847 2847
Size: 5843
EcoRV GAT'ATC
Cuts at: 1293 1293
Size: 5843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3862 3862
Size: 5843
FspI TGC'GCA
Cuts at: 3624 3624
Size: 5843
HaeII r_GCGC'y
Cuts at: 2724 3277 3525 4547 5526 2724
Size: 553 248 1022 979 3041
Fragments arranged by size:
3041 1022 979 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 1140 1172 1439 1471 2433 2465 3685
Size: 32 615 32 267 32 962 32 1220
Cuts at: 3685 3715 3717 3747 493
Size: 30 2 30 2589
Fragments arranged by size:
2589 1220 962 615 267 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 512 1863 2764 2882 512
Size: 1351 901 118 3473
Fragments arranged by size:
3473 1351 901 118
HpaI GTT'AAC
Cuts at: 1863 1863
Size: 5843
KasI G'GCGC_C
Cuts at: 3273 3521 3273
Size: 248 5595
KpnI G_GTAC'C
Cuts at: 2863 2863
Size: 5843
MluI A'CGCG_T
Cuts at: 5276 5276
Size: 5843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 614 4514 4698 614
Size: 3900 184 1759
Fragments arranged by size:
3900 1759 184
MscI TGG'CCA
Cuts at: 3604 3604
Size: 5843
MslI CAynn'nnrTG
Cuts at: 182 677 2257 3832 3959 182
Size: 495 1580 1575 127 2066
Fragments arranged by size:
2066 1580 1575 495 127
MspA1I CmG'CkG
Cuts at: 118 745 2140 2954 3268 3628 4641 4886 118
Size: 627 1395 814 314 360 1013 245 1075
Fragments arranged by size:
1395 1075 1013 814 627 360 314 245
NaeI GCC'GGC
Cuts at: 4024 4024
Size: 5843
NarI GG'CG_CC
Cuts at: 3274 3522 3274
Size: 248 5595
NcoI C'CATG_G
Cuts at: 107 612 2383 107
Size: 505 1771 3567
Fragments arranged by size:
3567 1771 505
NdeI CA'TA_TG
Cuts at: 130 130
Size: 5843
NgoMIV G'CCGG_C
Cuts at: 4022 4022
Size: 5843
NspI r_CATG'y
Cuts at: 443 643 976 1879 2416 2896 3396 4303 443
Size: 200 333 903 537 480 500 907 1983
Fragments arranged by size:
1983 907 903 537 500 480 333 200
PciI A'CATG_T
Cuts at: 972 4299 972
Size: 3327 2516
PmeI GTTT'AAAC
Cuts at: 2842 2842
Size: 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1670 1702 4982 5014 1670
Size: 32 3280 32 2499
Fragments arranged by size:
3280 2499 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 2446
Size: 5843
PspXI vC'TCGA_Gb
Cuts at: 1687 1687
Size: 5843
PstI C_TGCA'G
Cuts at: 2890 3575 2890
Size: 685 5158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5843
PvuII CAG'CTG
Cuts at: 745 2140 3268 3628 745
Size: 1395 1128 360 2960
Fragments arranged by size:
2960 1395 1128 360
RsrII CG'GwC_CG
Cuts at: 4038 4038
Size: 5843
SacI G_AGCT'C
Cuts at: 481 2132 2857 481
Size: 1651 725 3467
Fragments arranged by size:
3467 1651 725
SacII CC_GC'GG
Cuts at: 119 119
Size: 5843
SalI G'TCGA_C
Cuts at: 2880 2880
Size: 5843
SapI GCTCTTCn'nnn_
Cuts at: 911 3866 4076 911
Size: 2955 210 2678
Fragments arranged by size:
2955 2678 210
SbfI CC_TGCA'GG
Cuts at: 2890 2890
Size: 5843
ScaI AGT'ACT
Cuts at: 5360 5360
Size: 5843
SexAI A'CCwGG_T
Cuts at: 1449 1449
Size: 5843
SfcI C'TryA_G
Cuts at: 33 1656 2886 3571 4564 4755 33
Size: 1623 1230 685 993 191 1121
Fragments arranged by size:
1623 1230 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3275 3523 3275
Size: 248 5595
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
SmaI CCC'GGG
Cuts at: 2865 2865
Size: 5843
SmlI C'TyrA_G
Cuts at: 1687 2998 3913 4405 4667 1687
Size: 1311 915 492 262 2863
Fragments arranged by size:
2863 1311 915 492 262
SnaBI TAC'GTA
Cuts at: 1606 1606
Size: 5843
SpeI A'CTAG_T
Cuts at: 67 957 67
Size: 890 4953
SphI G_CATG'C
Cuts at: 443 643 1879 2416 2896 3396 443
Size: 200 1236 537 480 500 2890
Fragments arranged by size:
2890 1236 537 500 480 200
StuI AGG'CCT
Cuts at: 1103 1916 1103
Size: 813 5030
StyI C'CwwG_G
Cuts at: 107 612 1301 2357 2383 2977 3331 107
Size: 505 689 1056 26 594 354 2619
Fragments arranged by size:
2619 1056 689 594 505 354 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 134 920 972 3426 3559 4018 4227 134
Size: 786 52 2454 133 459 209 1750
Fragments arranged by size:
2454 1750 786 459 209 133 52
TatI w'GTAC_w
Cuts at: 190 1500 5358 190
Size: 1310 3858 675
Fragments arranged by size:
3858 1310 675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2390 2422 5360 5392 2204
Size: 32 154 32 2938 32 2655
Fragments arranged by size:
2938 2655 154 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3640 3640
Size: 5843
XbaI T'CTAG_A
Cuts at: 2874 2874
Size: 5843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2390 3338 2390
Size: 948 4895
XhoI C'TCGA_G
Cuts at: 1687 1687
Size: 5843
XmaI C'CCGG_G
Cuts at: 2863 2863
Size: 5843
XmnI GAAnn'nnTTC
Cuts at: 1916 1916
Size: 5843
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
FseI FspAI HindIII MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PsrI SanDI SfiI SgrAI SrfI SspI SwaI ZraI