Restriction map for
pF1KA0599
(Circular) (Six-base) MAPSORT of: pF1KA0599.seq Check: 6403 from: 1 to: 7133
pF1KA0599 7133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 821 1475 821
Size: 654 6479
AatII G_ACGT'C
Cuts at: 3620 3620
Size: 7133
AccI GT'mk_AC
Cuts at: 1121 3248 3372 4171 1121
Size: 2127 124 799 4083
Fragments arranged by size:
4083 2127 799 124
Acc65I G'GTAC_C
Cuts at: 1364 4149 1364
Size: 2785 4348
AclI AA'CG_TT
Cuts at: 6973 6973
Size: 7133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 802 1408 1604 2051 2655 2888 3588 4958
Size: 285 606 196 447 604 233 700 1370
Cuts at: 4958 5390 6137 6405 517
Size: 432 747 268 1245
Fragments arranged by size:
1370 1245 747 700 606 604 447 432
285 268 233 196
AflII C'TTAA_G
Cuts at: 2185 2185
Size: 7133
AflIII A'CryG_T
Cuts at: 4485 5589 6566 4485
Size: 1104 977 5052
Fragments arranged by size:
5052 1104 977
AgeI A'CCGG_T
Cuts at: 2564 3978 5521 2564
Size: 1414 1543 4176
Fragments arranged by size:
4176 1543 1414
AhdI GACnn_n'nnGTC
Cuts at: 2343 2343
Size: 7133
AleI CACnn'nnGTG
Cuts at: 1426 3318 1426
Size: 1892 5241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2407 2453 2407
Size: 46 7087
AlwNI CAG_nnn'CTG
Cuts at: 308 364 441 744 1582 2887 4438 6005 308
Size: 56 77 303 838 1305 1551 1567 1436
Fragments arranged by size:
1567 1551 1436 1305 838 303 77 56
ApaI G_GGCC'C
Cuts at: 329 992 2048 2826 329
Size: 663 1056 778 4636
Fragments arranged by size:
4636 1056 778 663
ApaLI G'TGCA_C
Cuts at: 5903 5903
Size: 7133
ApoI r'AATT_y
Cuts at: 4137 6486 6742 4137
Size: 2349 256 4528
Fragments arranged by size:
4528 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
AvaI C'yCGr_G
Cuts at: 487 1493 1562 1862 2603 3113 4065 4153 487
Size: 1006 69 300 741 510 952 88 3467
Fragments arranged by size:
3467 1006 952 741 510 300 88 69
BamHI G'GATC_C
Cuts at: 4158 4158
Size: 7133
BanI G'GyrC_C
Cuts at: 1364 1439 2224 3714 4149 4563 4811 4846 1364
Size: 75 785 1490 435 414 248 35 3651
Fragments arranged by size:
3651 1490 785 435 414 248 75 35
BanII G_rGCy'C
Cuts at: 190 224 329 772 992 1730 2048 2365 2534
Size: 34 105 443 220 738 318 317 169
Cuts at: 2534 2806 2826 2837 2953 3358 3583 3629 4147
Size: 272 20 11 116 405 225 46 518
Cuts at: 4147 5177 190
Size: 1030 2146
Fragments arranged by size:
2146 1030 738 518 443 405 318 317
272 225 220 169 116 105 46 34
20 11
BbeI G_GCGC'C
Cuts at: 1443 4567 4815 1443
Size: 3124 248 3761
Fragments arranged by size:
3761 3124 248
BbsI GAAGACnn'nnnn_
Cuts at: 2561 2930 2561
Size: 369 6764
BbvCI CC'TCA_GC
Cuts at: 2452 3586 2452
Size: 1134 5999
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1944 1978 90
Size: 34 1820 34 5245
Fragments arranged by size:
5245 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5031 5792 7133 5031
Size: 761 1341 5031
Fragments arranged by size:
5031 1341 761
BclI T'GATC_A
Cuts at: 4653 4653
Size: 7133
BfrBI ATG'CAT
Cuts at: 698 698
Size: 7133
BglI GCCn_nnn'nGGC
Cuts at: 2405 3109 3794 4076 2405
Size: 704 685 282 5462
Fragments arranged by size:
5462 704 685 282
BglII A'GATC_T
Cuts at: 2918 4648 6685 2918
Size: 1730 2037 3366
Fragments arranged by size:
3366 2037 1730
BlpI GC'TnA_GC
Cuts at: 379 2395 3142 4245 379
Size: 2016 747 1103 3267
Fragments arranged by size:
3267 2016 1103 747
Bme1580I G_kGCm'C
Cuts at: 329 992 1030 2048 2826 3368 4758 4851 5907
Size: 663 38 1018 778 542 1390 93 1056
Cuts at: 5907 329
Size: 1555
Fragments arranged by size:
1555 1390 1056 1018 778 663 542 93
38
BmrI ACTGGGnnnn_n'
Cuts at: 1916 2042 2545 4345 4426 4761 6905 1916
Size: 126 503 1800 81 335 2144 2144
Fragments arranged by size:
2144 2144 1800 503 335 126 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 3648 5500 2712
Size: 936 1852 4345
Fragments arranged by size:
4345 1852 936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 805 937 1042 1699 1887 2066 2113 2422
Size: 520 132 105 657 188 179 47 309
Cuts at: 2422 2718 2762 3037 3407 3597 3904 3967 285
Size: 296 44 275 370 190 307 63 3451
Fragments arranged by size:
3451 657 520 370 309 307 296 275
190 188 179 132 105 63 47 44
Bpu10I CC'TnA_GC
Cuts at: 1471 2194 2452 3586 3772 1471
Size: 723 258 1134 186 4832
Fragments arranged by size:
4832 1134 723 258 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 4309 5188 5680 5978 787
Size: 3522 879 492 298 1942
Fragments arranged by size:
3522 1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 563 1677 1937 2600 3592 3847 6837 563
Size: 1114 260 663 992 255 2990 859
Fragments arranged by size:
2990 1114 992 859 663 260 255
BsaAI yAC'GTr
Cuts at: 432 3207 3222 4486 432
Size: 2775 15 1264 3079
Fragments arranged by size:
3079 2775 1264 15
BsaBI GATnn'nnATC
Cuts at: 6 1156 4647 6684 6
Size: 1150 3491 2037 455
Fragments arranged by size:
3491 2037 1150 455
BsaHI Gr'CG_yC
Cuts at: 1440 2062 3617 4564 4812 5514 7010 1440
Size: 622 1555 947 248 702 1496 1563
Fragments arranged by size:
1563 1555 1496 947 702 622 248
BsaWI w'CCGG_w
Cuts at: 2564 2941 3275 3978 4322 4843 5521 5795 5942
Size: 377 334 703 344 521 678 274 147
Cuts at: 5942 6353 2564
Size: 411 3344
Fragments arranged by size:
3344 703 678 521 411 377 344 334
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 658 688 949 979 2064 2094 2716
Size: 30 345 30 261 30 1085 30 622
Cuts at: 2716 2746 283
Size: 30 4670
Fragments arranged by size:
4670 1085 622 345 261 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 168 180 232 553 942 961 1134 1261 1615
Size: 12 52 321 389 19 173 127 354
Cuts at: 1615 1630 1633 1645 1648 1651 1654 1657 1660
Size: 15 3 12 3 3 3 3 3
Cuts at: 1660 1663 1756 1759 1762 1765 1768 1777 2071
Size: 3 93 3 3 3 3 9 294
Cuts at: 2071 2218 3034 3061 3066 3474 3964 168
Size: 147 816 27 5 408 490 3337
Fragments arranged by size:
3337 816 490 408 389 354 321 294
173 147 127 93 52 27 19 15
12 12 9 5 3 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 223 1717 2008 3030 3490 3928 3990 4558 5316
Size: 1494 291 1022 460 438 62 568 758
Cuts at: 5316 5893 6389 223
Size: 577 496 967
Fragments arranged by size:
1494 1022 967 758 577 568 496 460
438 291 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 3163 3214 350
Size: 2813 51 4269
Fragments arranged by size:
4269 2813 51
BsiEI CG_ry'CG
Cuts at: 104 154 2812 3678 4675 4721 5929 104
Size: 50 2658 866 997 46 1208 1308
Fragments arranged by size:
2658 1308 1208 997 866 50 46
BsiHKAI G_wGCw'C
Cuts at: 941 1144 2176 2396 2837 3467 3629 3778 4147
Size: 203 1032 220 441 630 162 149 369
Cuts at: 4147 4925 5115 5907 941
Size: 778 190 792 2167
Fragments arranged by size:
2167 1032 792 778 630 441 369 220
203 190 162 149
BsmI GAATG_Cn'
Cuts at: 700 700
Size: 7133
BsmBI CGTCTCn'nnnn_
Cuts at: 2053 2053
Size: 7133
Bsp1286I G_dGCh'C
Cuts at: 190 224 329 772 941 992 1030 1144 1730
Size: 34 105 443 169 51 38 114 586
Cuts at: 1730 2048 2176 2365 2396 2534 2806 2826 2837
Size: 318 128 189 31 138 272 20 11
Cuts at: 2837 2953 3358 3368 3467 3583 3629 3778 4147
Size: 116 405 10 99 116 46 149 369
Cuts at: 4147 4758 4851 4925 5115 5177 5907 190
Size: 611 93 74 190 62 730 1416
Fragments arranged by size:
1416 730 611 586 443 405 369 318
272 190 189 169 149 138 128 116
116 114 105 99 93 74 62 51
46 38 34 31 20 11 10
BspEI T'CCGG_A
Cuts at: 2941 3275 6353 2941
Size: 334 3078 3721
Fragments arranged by size:
3721 3078 334
BspHI T'CATG_A
Cuts at: 6309 6309
Size: 7133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 785 821 1475 1776 3002 3987 4183 4397
Size: 336 36 654 301 1226 985 196 214
Cuts at: 4397 4699 5080 449
Size: 302 381 2502
Fragments arranged by size:
2502 1226 985 654 381 336 302 301
214 196 36
BsrBI CCG'CTC
Cuts at: 50 150 1383 5425 5479 6963 7128 50
Size: 100 1233 4042 54 1484 165 55
Fragments arranged by size:
4042 1484 1233 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 1059 2282 5045 1059
Size: 1223 2763 3147
Fragments arranged by size:
3147 2763 1223
BsrFI r'CCGG_y
Cuts at: 153 272 734 1442 1928 2564 2609 3420 3563
Size: 119 462 708 486 636 45 811 143
Cuts at: 3563 3677 3893 3978 5131 5312 5521 153
Size: 114 216 85 1153 181 209 1765
Fragments arranged by size:
1765 1153 811 708 636 486 462 216
209 181 143 119 114 85 45
BssSI C'ACGA_G
Cuts at: 5404 5762 5404
Size: 358 6775
BstAPI GCAn_nnn'nTGC
Cuts at: 364 744 759 1047 1522 4510 364
Size: 380 15 288 475 2988 2987
Fragments arranged by size:
2988 2987 475 380 288 15
BstBI TT'CG_AA
Cuts at: 5494 5494
Size: 7133
BstXI CCAn_nnnn'nTGG
Cuts at: 136 678 1242 2715 2983 3320 3817 136
Size: 542 564 1473 268 337 497 3452
Fragments arranged by size:
3452 1473 564 542 497 337 268
BstYI r'GATC_y
Cuts at: 1 2918 4158 4625 4648 4983 5229 6241 6327
Size: 2917 1240 467 23 335 246 1012 86
Cuts at: 6327 6339 6685 1
Size: 12 346 449
Fragments arranged by size:
2917 1240 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2003 2003
Size: 7133
BtgI C'CryG_G
Cuts at: 212 596 677 2365 3684 212
Size: 384 81 1688 1319 3661
Fragments arranged by size:
3661 1688 1319 384 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1027 3665 5083 5264 5427 1027
Size: 2638 1418 181 163 2733
Fragments arranged by size:
2733 2638 1418 181 163
BtsI GCAGTG_nn'
Cuts at: 213 1084 1527 1824 2442 3056 3175 3313 3325
Size: 871 443 297 618 614 119 138 12
Cuts at: 3325 3866 3935 3963 4376 213
Size: 541 69 28 413 2970
Fragments arranged by size:
2970 871 618 614 541 443 413 297
138 119 69 28 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2331 2366 2331
Size: 35 7098
DraI TTT'AAA
Cuts at: 4132 4132
Size: 7133
DraIII CAC_nnn'GTG
Cuts at: 3714 3714
Size: 7133
DrdI GACnn_nn'nnGTC
Cuts at: 4839 5697 4839
Size: 858 6275
EaeI y'GGCC_r
Cuts at: 151 605 680 1814 1926 2495 2755 2809 3053
Size: 454 75 1134 112 569 260 54 244
Cuts at: 3053 3302 3675 3960 4718 4892 5283 5310 6655
Size: 249 373 285 758 174 391 27 1345
Cuts at: 6655 151
Size: 629
Fragments arranged by size:
1345 1134 758 629 569 454 391 373
285 260 249 244 174 112 75 54
27
EagI C'GGCC_G
Cuts at: 151 2809 3675 4718 151
Size: 2658 866 1043 2566
Fragments arranged by size:
2658 2566 1043 866
EarI CTCTTCn'nnn_
Cuts at: 845 919 1601 1607 1649 3013 3346 3374 4034
Size: 74 682 6 42 1364 333 28 660
Cuts at: 4034 5156 5366 845
Size: 1122 210 2612
Fragments arranged by size:
2612 1364 1122 682 660 333 210 74
42 28 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1795 2331 2884 3561 3812 5341 5651 5797 6943
Size: 536 553 677 251 1529 310 146 1146
Cuts at: 6943 1795
Size: 1985
Fragments arranged by size:
1985 1529 1146 677 553 536 310 251
146
EcoICRI GAG'CTC
Cuts at: 2835 3627 4145 2835
Size: 792 518 5823
Fragments arranged by size:
5823 792 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 517 802 805 937 1042 1408 1604 1699
Size: 232 285 3 132 105 366 196 95
Cuts at: 1699 1887 2051 2066 2113 2422 2655 2718 2762
Size: 188 164 15 47 309 233 63 44
Cuts at: 2762 2888 3037 3407 3588 3597 3904 3967 4958
Size: 126 149 370 181 9 307 63 991
Cuts at: 4958 5390 6137 6405 285
Size: 432 747 268 1013
Fragments arranged by size:
1013 991 747 432 370 366 309 307
285 268 233 232 196 188 181 164
149 132 126 105 95 63 63 47
44 15 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 130 1984 130
Size: 1854 5279
EcoO109I rG'GnC_Cy
Cuts at: 325 326 361 438 988 1061 1566 1683 2044
Size: 1 35 77 550 73 505 117 361
Cuts at: 2044 2118 2181 2190 2418 2823 2865 3919 4053
Size: 74 63 9 228 405 42 1054 134
Cuts at: 4053 4272 325
Size: 219 3186
Fragments arranged by size:
3186 1054 550 505 405 361 228 219
134 117 77 74 73 63 42 35
9 1
EcoRI G'AATT_C
Cuts at: 4137 4137
Size: 7133
EcoRV GAT'ATC
Cuts at: 642 642
Size: 7133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5152 5152
Size: 7133
FseI GG_CCGG'CC
Cuts at: 3567 3681 3567
Size: 114 7019
FspI TGC'GCA
Cuts at: 445 1051 4914 445
Size: 606 3863 2664
Fragments arranged by size:
3863 2664 606
HaeII r_GCGC'y
Cuts at: 518 1443 1871 4567 4815 5837 6816 518
Size: 925 428 2696 248 1022 979 835
Fragments arranged by size:
2696 1022 979 925 835 428 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 949 981 1402 1434 2062 2094 2329 2361 4975
Size: 32 421 32 628 32 235 32 2614
Cuts at: 4975 5005 5007 5037 949
Size: 30 2 30 3045
Fragments arranged by size:
3045 2614 628 421 235 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3699 4172 3699
Size: 473 6660
KasI G'GCGC_C
Cuts at: 1439 4563 4811 1439
Size: 3124 248 3761
Fragments arranged by size:
3761 3124 248
KpnI G_GTAC'C
Cuts at: 1368 4153 1368
Size: 2785 4348
MluI A'CGCG_T
Cuts at: 6566 6566
Size: 7133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1960 5804 5988 1960
Size: 3844 184 3105
Fragments arranged by size:
3844 3105 184
MscI TGG'CCA
Cuts at: 607 2497 3304 3962 4894 607
Size: 1890 807 658 932 2846
Fragments arranged by size:
2846 1890 932 807 658
MslI CAynn'nnrTG
Cuts at: 112 134 842 1426 2435 2485 2660 3318 5122
Size: 22 708 584 1009 50 175 658 1804
Cuts at: 5122 5249 112
Size: 127 1996
Fragments arranged by size:
1996 1804 1009 708 658 584 175 127
50 22
MspA1I CmG'CkG
Cuts at: 308 355 581 611 1370 2167 2367 2456 3255
Size: 47 226 30 759 797 200 89 799
Cuts at: 3255 3450 3608 3686 3761 4244 4558 4918 5931
Size: 195 158 78 75 483 314 360 1013
Cuts at: 5931 6176 308
Size: 245 1265
Fragments arranged by size:
1265 1013 799 797 759 483 360 314
245 226 200 195 158 89 78 75
47 30
NaeI GCC'GGC
Cuts at: 274 1444 3565 3679 5314 274
Size: 1170 2121 114 1635 2093
Fragments arranged by size:
2121 2093 1635 1170 114
NarI GG'CG_CC
Cuts at: 1440 4564 4812 1440
Size: 3124 248 3761
Fragments arranged by size:
3761 3124 248
NcoI C'CATG_G
Cuts at: 212 212
Size: 7133
NgoMIV G'CCGG_C
Cuts at: 272 1442 3563 3677 5312 272
Size: 1170 2121 114 1635 2093
Fragments arranged by size:
2121 2093 1635 1170 114
NsiI A_TGCA'T
Cuts at: 700 700
Size: 7133
NspI r_CATG'y
Cuts at: 429 702 1516 4186 4686 5593 429
Size: 273 814 2670 500 907 1969
Fragments arranged by size:
2670 1969 907 814 500 273
PasI CC'CwG_GG
Cuts at: 2953 3156 3817 2953
Size: 203 661 6269
Fragments arranged by size:
6269 661 203
PciI A'CATG_T
Cuts at: 5589 5589
Size: 7133
PflMI CCAn_nnn'nTGG
Cuts at: 308 3210 3400 308
Size: 2902 190 4041
Fragments arranged by size:
4041 2902 190
PfoI T'CCnGG_A
Cuts at: 821 953 1019 1535 1903 2090 3014 821
Size: 132 66 516 368 187 924 4940
Fragments arranged by size:
4940 924 516 368 187 132 66
PmeI GTTT'AAAC
Cuts at: 4132 4132
Size: 7133
PmlI CAC'GTG
Cuts at: 3222 3222
Size: 7133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6272 6304 6272
Size: 32 7101
PpuMI rG'GwC_Cy
Cuts at: 438 1061 1683 2181 2865 3919 4053 438
Size: 623 622 498 684 1054 134 3518
Fragments arranged by size:
3518 1054 684 623 622 498 134
PspOMI G'GGCC_C
Cuts at: 325 988 2044 2822 325
Size: 663 1056 778 4636
Fragments arranged by size:
4636 1056 778 663
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3232 3264 3232
Size: 32 7101
PstI C_TGCA'G
Cuts at: 1375 1574 3320 3873 3942 4023 4180 4865 1375
Size: 199 1746 553 69 81 157 685 3643
Fragments arranged by size:
3643 1746 685 553 199 157 81 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7133
PvuII CAG'CTG
Cuts at: 308 581 611 2167 2456 3255 3450 3608 4558
Size: 273 30 1556 289 799 195 158 950
Cuts at: 4558 4918 308
Size: 360 2523
Fragments arranged by size:
2523 1556 950 799 360 289 273 195
158 30
RsrII CG'GwC_CG
Cuts at: 945 5328 945
Size: 4383 2750
SacI G_AGCT'C
Cuts at: 2837 3629 4147 2837
Size: 792 518 5823
Fragments arranged by size:
5823 792 518
SacII CC_GC'GG
Cuts at: 2368 3687 2368
Size: 1319 5814
SalI G'TCGA_C
Cuts at: 4170 4170
Size: 7133
SanDI GG'GwC_CC
Cuts at: 2865 3919 4053 2865
Size: 1054 134 5945
Fragments arranged by size:
5945 1054 134
SapI GCTCTTCn'nnn_
Cuts at: 1649 3346 5156 5366 1649
Size: 1697 1810 210 3416
Fragments arranged by size:
3416 1810 1697 210
SbfI CC_TGCA'GG
Cuts at: 4180 4180
Size: 7133
ScaI AGT'ACT
Cuts at: 1944 6650 1944
Size: 4706 2427
SfcI C'TryA_G
Cuts at: 33 748 1371 1570 3316 3373 3869 3938 4019
Size: 715 623 199 1746 57 496 69 81
Cuts at: 4019 4176 4861 5854 6045 33
Size: 157 685 993 191 1121
Fragments arranged by size:
1746 1121 993 715 685 623 496 199
191 157 81 69 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 3109 3109
Size: 7133
SfoI GGC'GCC
Cuts at: 1441 4565 4813 1441
Size: 3124 248 3761
Fragments arranged by size:
3761 3124 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7133
SgrAI Cr'CCGG_yG
Cuts at: 3978 3978
Size: 7133
SmaI CCC'GGG
Cuts at: 489 1864 3115 4155 489
Size: 1375 1251 1040 3467
Fragments arranged by size:
3467 1375 1251 1040
SmlI C'TyrA_G
Cuts at: 802 2185 4288 5203 5695 5957 802
Size: 1383 2103 915 492 262 1978
Fragments arranged by size:
2103 1978 1383 915 492 262
SnaBI TAC'GTA
Cuts at: 432 432
Size: 7133
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7133
SphI G_CATG'C
Cuts at: 702 1516 4186 4686 702
Size: 814 2670 500 3149
Fragments arranged by size:
3149 2670 814 500
StuI AGG'CCT
Cuts at: 1663 4111 1663
Size: 2448 4685
StyI C'CwwG_G
Cuts at: 212 764 1322 4267 4621 212
Size: 552 558 2945 354 2724
Fragments arranged by size:
2945 2724 558 552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1210 4716 4849 5308 5517 1210
Size: 3506 133 459 209 2826
Fragments arranged by size:
3506 2826 459 209 133
TatI w'GTAC_w
Cuts at: 1220 1942 3266 6648 1220
Size: 722 1324 3382 1705
Fragments arranged by size:
3382 1705 1324 722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 2996 3028 6650 6682 2064
Size: 32 900 32 3622 32 2515
Fragments arranged by size:
3622 2515 900 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4930 4930
Size: 7133
XbaI T'CTAG_A
Cuts at: 4164 4164
Size: 7133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 615 897 1338 1809 3317 3942 4628 615
Size: 282 441 471 1508 625 686 3120
Fragments arranged by size:
3120 1508 686 625 471 441 282
XmaI C'CCGG_G
Cuts at: 487 1862 3113 4153 487
Size: 1375 1251 1040 3467
Fragments arranged by size:
3467 1375 1251 1040
ZraI GAC'GTC
Cuts at: 3618 3618
Size: 7133
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BmgBI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PshAI PsiI
PspXI SexAI SrfI SspI SwaI XhoI XmnI