Restriction map for
pF1KA0600
(Circular) (Six-base) MAPSORT of: pF1KA0600.seq Check: 7094 from: 1 to: 6224
pF1KA0600 6224 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2016 3073 2016
Size: 1057 5167
AccI GT'mk_AC
Cuts at: 1288 3262 1288
Size: 1974 4250
Acc65I G'GTAC_C
Cuts at: 3240 3240
Size: 6224
AclI AA'CG_TT
Cuts at: 6064 6064
Size: 6224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2658 4049 4481 4834 5496 2594
Size: 64 1391 432 353 662 3322
Fragments arranged by size:
3322 1391 662 432 353 64
AflIII A'CryG_T
Cuts at: 1199 1533 3576 5376 5657 1199
Size: 334 2043 1800 281 1766
Fragments arranged by size:
2043 1800 1766 334 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2286 3227 2286
Size: 941 5283
AlwNI CAG_nnn'CTG
Cuts at: 333 372 387 528 585 597 883 1326 1629
Size: 39 15 141 57 12 286 443 303
Cuts at: 1629 1638 1668 2013 2073 2563 3529 4967 333
Size: 9 30 345 60 490 966 1438 1590
Fragments arranged by size:
1590 1438 966 490 443 345 303 286
141 60 57 39 30 15 12 9
ApaI G_GGCC'C
Cuts at: 842 1958 842
Size: 1116 5108
ApaLI G'TGCA_C
Cuts at: 5062 5062
Size: 6224
ApoI r'AATT_y
Cuts at: 673 3228 5577 5833 673
Size: 2555 2349 256 1064
Fragments arranged by size:
2555 2349 1064 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6224
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
AvaI C'yCGr_G
Cuts at: 1823 3164 3244 1823
Size: 1341 80 4803
Fragments arranged by size:
4803 1341 80
AvrII C'CTAG_G
Cuts at: 1227 1227
Size: 6224
BamHI G'GATC_C
Cuts at: 3249 3249
Size: 6224
BanI G'GyrC_C
Cuts at: 1012 2037 2479 3240 3654 3902 3937 1012
Size: 1025 442 761 414 248 35 3299
Fragments arranged by size:
3299 1025 761 442 414 248 35
BanII G_rGCy'C
Cuts at: 294 304 347 701 756 842 1161 1420 1812
Size: 10 43 354 55 86 319 259 392
Cuts at: 1812 1834 1958 2122 2312 2571 3197 3238 4268
Size: 22 124 164 190 259 626 41 1030
Cuts at: 4268 294
Size: 2250
Fragments arranged by size:
2250 1030 626 392 354 319 259 259
190 164 124 86 55 43 41 22
10
BbeI G_GCGC'C
Cuts at: 2041 3658 3906 2041
Size: 1617 248 4359
Fragments arranged by size:
4359 1617 248
BbsI GAAGACnn'nnnn_
Cuts at: 1865 1889 1865
Size: 24 6200
BbvCI CC'TCA_GC
Cuts at: 661 1148 1946 2044 661
Size: 487 798 98 4841
Fragments arranged by size:
4841 798 487 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1924 1958 1924
Size: 34 6190
BciVI GTATCCnnnnn_n'
Cuts at: 1975 4122 5178 6224 1975
Size: 2147 1056 1046 1975
Fragments arranged by size:
2147 1975 1056 1046
BclI T'GATC_A
Cuts at: 3744 3744
Size: 6224
BglI GCCn_nnn'nGGC
Cuts at: 1391 2899 3145 1391
Size: 1508 246 4470
Fragments arranged by size:
4470 1508 246
BglII A'GATC_T
Cuts at: 3739 5776 3739
Size: 2037 4187
BlpI GC'TnA_GC
Cuts at: 929 3336 929
Size: 2407 3817
Bme1580I G_kGCm'C
Cuts at: 842 1958 2063 3849 3942 5066 842
Size: 1116 105 1786 93 1124 2000
Fragments arranged by size:
2000 1786 1124 1116 105 93
BmgBI CAC'GTC
Cuts at: 1202 2655 1202
Size: 1453 4771
BmrI ACTGGGnnnn_n'
Cuts at: 230 2079 2466 3436 3517 3852 5996 230
Size: 1849 387 970 81 335 2144 458
Fragments arranged by size:
2144 1849 970 458 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 800 1610 2256 4591 800
Size: 810 646 2335 2433
Fragments arranged by size:
2433 2335 810 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 592 607 652 1303 1423 1480 1690 1885
Size: 42 15 45 651 120 57 210 195
Cuts at: 1885 2229 2299 2878 3045 550
Size: 344 70 579 167 3729
Fragments arranged by size:
3729 651 579 344 210 195 167 120
70 57 45 42 15
Bpu10I CC'TnA_GC
Cuts at: 661 1148 1585 1946 2044 661
Size: 487 437 361 98 4841
Fragments arranged by size:
4841 487 437 361 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 442 2296 3400 4279 4993 5291 340
Size: 102 1854 1104 879 714 298 1273
Fragments arranged by size:
1854 1273 1104 879 714 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 3082 5928 3082
Size: 2846 3378
BsaAI yAC'GTr
Cuts at: 3577 3577
Size: 6224
BsaBI GATnn'nnATC
Cuts at: 6 3738 5775 6
Size: 3732 2037 455
Fragments arranged by size:
3732 2037 455
BsaHI Gr'CG_yC
Cuts at: 808 1027 2038 3655 3903 4605 6101 808
Size: 219 1011 1617 248 702 1496 931
Fragments arranged by size:
1617 1496 1011 931 702 248 219
BsaWI w'CCGG_w
Cuts at: 3413 3934 4612 5023 5170 5444 3413
Size: 521 678 411 147 274 4193
Fragments arranged by size:
4193 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 927 957 927
Size: 30 6194
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1771 1780 1783 1882 1885 1909 1912 1915 1942
Size: 9 3 99 3 24 3 3 27
Cuts at: 1942 1963 1981 2232 2756 3109 3205 1771
Size: 21 18 251 524 353 96 4790
Fragments arranged by size:
4790 524 353 251 99 96 27 24
21 18 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 268 746 1063 1389 1698 1797 2006 2483 3649
Size: 478 317 326 309 99 209 477 1166
Cuts at: 3649 4407 5072 5480 268
Size: 758 665 408 1012
Fragments arranged by size:
1166 1012 758 665 478 477 408 326
317 309 209 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 3055 166
Size: 2889 3335
BsiEI CG_ry'CG
Cuts at: 104 3766 3812 5042 104
Size: 3662 46 1230 1286
Fragments arranged by size:
3662 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 347 1366 1483 1852 2312 2516 3238 4016 4206
Size: 1019 117 369 460 204 722 778 190
Cuts at: 4206 5066 347
Size: 860 1505
Fragments arranged by size:
1505 1019 860 778 722 460 369 204
190 117
BsmBI CGTCTCn'nnnn_
Cuts at: 848 1208 2645 848
Size: 360 1437 4427
Fragments arranged by size:
4427 1437 360
Bsp1286I G_dGCh'C
Cuts at: 294 304 347 701 756 842 1161 1366 1420
Size: 10 43 354 55 86 319 205 54
Cuts at: 1420 1483 1812 1834 1852 1958 2063 2122 2312
Size: 63 329 22 18 106 105 59 190
Cuts at: 2312 2516 2571 3197 3238 3849 3942 4016 4206
Size: 204 55 626 41 611 93 74 190
Cuts at: 4206 4268 5066 294
Size: 62 798 1452
Fragments arranged by size:
1452 798 626 611 354 329 319 205
204 190 190 106 105 93 86 74
63 62 59 55 55 54 43 41
22 18 10
BspHI T'CATG_A
Cuts at: 1358 4656 1358
Size: 3298 2926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2016 3073 3274 3488 3790 4171 2016
Size: 1057 201 214 302 381 4069
Fragments arranged by size:
4069 1057 381 302 214 201
BsrBI CCG'CTC
Cuts at: 50 794 933 4516 4570 6054 6219 50
Size: 744 139 3583 54 1484 165 55
Fragments arranged by size:
3583 1484 744 165 139 55 54
BsrDI GCAATG_nn'
Cuts at: 638 1487 2481 2693 4136 638
Size: 849 994 212 1443 2726
Fragments arranged by size:
2726 1443 994 849 212
BsrFI r'CCGG_y
Cuts at: 213 1348 3059 3183 4222 4403 213
Size: 1135 1711 124 1039 181 2034
Fragments arranged by size:
2034 1711 1135 1039 181 124
BssSI C'ACGA_G
Cuts at: 2701 4495 5203 2701
Size: 1794 708 3722
Fragments arranged by size:
3722 1794 708
BstAPI GCAn_nnn'nTGC
Cuts at: 333 372 474 501 597 1449 2022 3601 333
Size: 39 102 27 96 852 573 1579 2956
Fragments arranged by size:
2956 1579 852 573 102 96 39 27
BstBI TT'CG_AA
Cuts at: 4585 4585
Size: 6224
BstXI CCAn_nnnn'nTGG
Cuts at: 243 276 2370 2476 243
Size: 33 2094 106 3991
Fragments arranged by size:
3991 2094 106 33
BstYI r'GATC_y
Cuts at: 1 1706 3008 3249 3716 3739 4074 4320 4626
Size: 1705 1302 241 467 23 335 246 306
Cuts at: 4626 4638 4724 5776 1
Size: 12 86 1052 449
Fragments arranged by size:
1705 1302 1052 467 449 335 306 246
241 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1754 1754
Size: 6224
BtgI C'CryG_G
Cuts at: 169 242 1241 1422 1503 1570 2369 2469 3116
Size: 73 999 181 81 67 799 100 647
Cuts at: 3116 3197 169
Size: 81 3196
Fragments arranged by size:
3196 999 799 647 181 100 81 81
73 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1077 2515 4174 4355 4518 1077
Size: 1438 1659 181 163 2783
Fragments arranged by size:
2783 1659 1438 181 163
BtsI GCAGTG_nn'
Cuts at: 316 1268 2270 2987 3467 316
Size: 952 1002 717 480 3073
Fragments arranged by size:
3073 1002 952 717 480
DraI TTT'AAA
Cuts at: 3223 3223
Size: 6224
DraIII CAC_nnn'GTG
Cuts at: 2225 2225
Size: 6224
DrdI GACnn_nn'nnGTC
Cuts at: 2134 3930 5274 2134
Size: 1796 1344 3084
Fragments arranged by size:
3084 1796 1344
EaeI y'GGCC_r
Cuts at: 1538 2051 2802 2984 3809 3983 4374 4401 5746
Size: 513 751 182 825 174 391 27 1345
Cuts at: 5746 1538
Size: 2016
Fragments arranged by size:
2016 1345 825 751 513 391 182 174
27
EagI C'GGCC_G
Cuts at: 3809 3809
Size: 6224
EarI CTCTTCn'nnn_
Cuts at: 560 616 985 2110 2152 2570 4247 4457 560
Size: 56 369 1125 42 418 1677 210 2327
Fragments arranged by size:
2327 1677 1125 418 369 210 56 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2721 4432 5174 5320 6034 2721
Size: 1711 742 146 714 2911
Fragments arranged by size:
2911 1711 742 714 146
EcoICRI GAG'CTC
Cuts at: 345 2310 3236 345
Size: 1965 926 3333
Fragments arranged by size:
3333 1965 926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 592 607 652 1303 1423 1480 1690 1885
Size: 42 15 45 651 120 57 210 195
Cuts at: 1885 2229 2299 2594 2658 2878 3045 4049 4481
Size: 344 70 295 64 220 167 1004 432
Cuts at: 4481 4834 5496 550
Size: 353 662 1278
Fragments arranged by size:
1278 1004 662 651 432 353 344 295
220 210 195 167 120 70 64 57
45 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1330 1867 3070 1330
Size: 537 1203 4484
Fragments arranged by size:
4484 1203 537
EcoO109I rG'GnC_Cy
Cuts at: 839 1314 1626 1954 2070 2158 2442 3363 839
Size: 475 312 328 116 88 284 921 3700
Fragments arranged by size:
3700 921 475 328 312 284 116 88
EcoRI G'AATT_C
Cuts at: 673 3228 673
Size: 2555 3669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2163 4243 2163
Size: 2080 4144
FspI TGC'GCA
Cuts at: 2257 4005 2257
Size: 1748 4476
FspAI rTGC'GCAy
Cuts at: 2257 2257
Size: 6224
HaeII r_GCGC'y
Cuts at: 355 2041 3116 3658 3906 5136 5907 355
Size: 1686 1075 542 248 1230 771 672
Fragments arranged by size:
1686 1230 1075 771 672 542 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 126 153 158 927 959 1131 1163 2312
Size: 5 27 5 769 32 172 32 1149
Cuts at: 2312 2344 4066 4096 4098 4128 121
Size: 32 1722 30 2 30 2217
Fragments arranged by size:
2217 1722 1149 769 172 32 32 32
30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1289 3263 1289
Size: 1974 4250
KasI G'GCGC_C
Cuts at: 2037 3654 3902 2037
Size: 1617 248 4359
Fragments arranged by size:
4359 1617 248
KpnI G_GTAC'C
Cuts at: 3244 3244
Size: 6224
MluI A'CGCG_T
Cuts at: 5657 5657
Size: 6224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 4983 5167 107
Size: 4876 184 1164
Fragments arranged by size:
4876 1164 184
MscI TGG'CCA
Cuts at: 1540 2053 2804 2986 3985 1540
Size: 513 751 182 999 3779
Fragments arranged by size:
3779 999 751 513 182
MslI CAynn'nnrTG
Cuts at: 193 1138 1427 1492 1546 2206 2263 2368 2474
Size: 945 289 65 54 660 57 105 106
Cuts at: 2474 2534 4213 4340 193
Size: 60 1679 127 2077
Fragments arranged by size:
2077 1679 945 660 289 127 106 105
65 60 57 54
MspA1I CmG'CkG
Cuts at: 171 372 444 540 546 558 582 597 1572
Size: 201 72 96 6 12 24 15 975
Cuts at: 1572 1608 1638 1698 2013 2820 3049 3335 3649
Size: 36 30 60 315 807 229 286 314
Cuts at: 3649 4009 4793 5038 171
Size: 360 784 245 1357
Fragments arranged by size:
1357 975 807 784 360 315 314 286
245 229 201 96 72 60 36 30
24 15 12 6
NaeI GCC'GGC
Cuts at: 3185 4405 3185
Size: 1220 5004
NarI GG'CG_CC
Cuts at: 2038 3655 3903 2038
Size: 1617 248 4359
Fragments arranged by size:
4359 1617 248
NcoI C'CATG_G
Cuts at: 242 2369 2469 3116 3197 242
Size: 2127 100 647 81 3269
Fragments arranged by size:
3269 2127 647 100 81
NgoMIV G'CCGG_C
Cuts at: 3183 4403 3183
Size: 1220 5004
NspI r_CATG'y
Cuts at: 1432 1450 1551 3277 3777 5380 1432
Size: 18 101 1726 500 1603 2276
Fragments arranged by size:
2276 1726 1603 500 101 18
PasI CC'CwG_GG
Cuts at: 802 2073 2104 802
Size: 1271 31 4922
Fragments arranged by size:
4922 1271 31
PciI A'CATG_T
Cuts at: 5376 5376
Size: 6224
PflMI CCAn_nnn'nTGG
Cuts at: 746 2073 2563 746
Size: 1327 490 4407
Fragments arranged by size:
4407 1327 490
PfoI T'CCnGG_A
Cuts at: 668 1667 668
Size: 999 5225
PmeI GTTT'AAAC
Cuts at: 3223 3223
Size: 6224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 6192
PpuMI rG'GwC_Cy
Cuts at: 2442 2442
Size: 6224
PshAI GACnn'nnGTC
Cuts at: 2760 2760
Size: 6224
PspOMI G'GGCC_C
Cuts at: 838 1954 838
Size: 1116 5108
PstI C_TGCA'G
Cuts at: 338 377 449 1238 1445 1634 2018 2304 2936
Size: 39 72 789 207 189 384 286 632
Cuts at: 2936 3271 3956 338
Size: 335 685 2606
Fragments arranged by size:
2606 789 685 632 384 335 286 207
189 72 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6224
PvuII CAG'CTG
Cuts at: 372 444 597 1638 1698 2820 3649 4009 372
Size: 72 153 1041 60 1122 829 360 2587
Fragments arranged by size:
2587 1122 1041 829 360 153 72 60
RsrII CG'GwC_CG
Cuts at: 4419 4419
Size: 6224
SacI G_AGCT'C
Cuts at: 347 2312 3238 347
Size: 1965 926 3333
Fragments arranged by size:
3333 1965 926
SacII CC_GC'GG
Cuts at: 172 1573 172
Size: 1401 4823
SalI G'TCGA_C
Cuts at: 1287 3261 1287
Size: 1974 4250
SapI GCTCTTCn'nnn_
Cuts at: 560 616 2110 4247 4457 560
Size: 56 1494 2137 210 2327
Fragments arranged by size:
2327 2137 1494 210 56
SbfI CC_TGCA'GG
Cuts at: 3271 3271
Size: 6224
ScaI AGT'ACT
Cuts at: 5741 5741
Size: 6224
SfcI C'TryA_G
Cuts at: 33 334 373 445 1234 1441 1630 1693 2014
Size: 301 39 72 789 207 189 63 321
Cuts at: 2014 2300 2393 2416 2932 3267 3952 4920 5111
Size: 286 93 23 516 335 685 968 191
Cuts at: 5111 33
Size: 1146
Fragments arranged by size:
1146 968 789 685 516 335 321 301
286 207 191 189 93 72 63 39
23
SfiI GGCCn_nnn'nGGCC
Cuts at: 3145 3145
Size: 6224
SfoI GGC'GCC
Cuts at: 2039 3656 3904 2039
Size: 1617 248 4359
Fragments arranged by size:
4359 1617 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
SmaI CCC'GGG
Cuts at: 1825 3166 3246 1825
Size: 1341 80 4803
Fragments arranged by size:
4803 1341 80
SmlI C'TyrA_G
Cuts at: 355 457 2311 3379 4294 5008 5270 355
Size: 102 1854 1068 915 714 262 1309
Fragments arranged by size:
1854 1309 1068 915 714 262 102
SpeI A'CTAG_T
Cuts at: 67 1518 67
Size: 1451 4773
SphI G_CATG'C
Cuts at: 1432 1551 3277 3777 1432
Size: 119 1726 500 3879
Fragments arranged by size:
3879 1726 500 119
StyI C'CwwG_G
Cuts at: 155 242 1227 2369 2469 2939 3116 3197 3358
Size: 87 985 1142 100 470 177 81 161
Cuts at: 3358 3712 155
Size: 354 2667
Fragments arranged by size:
2667 1142 985 470 354 177 161 100
87 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1762 2497 2761 3106 3807 3940 4399 4608 1762
Size: 735 264 345 701 133 459 209 3378
Fragments arranged by size:
3378 735 701 459 345 264 209 133
TatI w'GTAC_w
Cuts at: 5739 5739
Size: 6224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1495 1527 2582 2614 2616 2648 5741 5773 1495
Size: 32 1055 32 2 32 3093 32 1946
Fragments arranged by size:
3093 1946 1055 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 4021 4021
Size: 6224
XbaI T'CTAG_A
Cuts at: 3255 3255
Size: 6224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2652 3719 2652
Size: 1067 5157
XmaI C'CCGG_G
Cuts at: 1823 3164 3244 1823
Size: 1341 80 4803
Fragments arranged by size:
4803 1341 80
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
BaeI BfrBI BmtI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI