Restriction map for
pF1KA0602
(Circular) (Six-base) MAPSORT of: pF1KA0602.seq Check: 353 from: 1 to: 5825
pF1KA0602 5825 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2385 2385
Size: 5825
AatII G_ACGT'C
Cuts at: 838 1640 1691 2735 838
Size: 802 51 1044 3928
Fragments arranged by size:
3928 1044 802 51
AccI GT'mk_AC
Cuts at: 2863 2863
Size: 5825
Acc65I G'GTAC_C
Cuts at: 1907 2841 1907
Size: 934 4891
AclI AA'CG_TT
Cuts at: 5665 5665
Size: 5825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 424 569 1686 3650 4082 4829 5097 265
Size: 159 145 1117 1964 432 747 268 993
Fragments arranged by size:
1964 1117 993 747 432 268 159 145
AfeI AGC'GCT
Cuts at: 466 878 1480 466
Size: 412 602 4811
Fragments arranged by size:
4811 602 412
AflIII A'CryG_T
Cuts at: 3177 4281 5258 3177
Size: 1104 977 3744
Fragments arranged by size:
3744 1104 977
AgeI A'CCGG_T
Cuts at: 4213 4213
Size: 5825
AhdI GACnn_n'nnGTC
Cuts at: 2019 2151 2628 2019
Size: 132 477 5216
Fragments arranged by size:
5216 477 132
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 5825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 947 947
Size: 5825
AlwNI CAG_nnn'CTG
Cuts at: 564 1953 3130 4697 564
Size: 1389 1177 1567 1692
Fragments arranged by size:
1692 1567 1389 1177
ApaLI G'TGCA_C
Cuts at: 1668 2484 4595 1668
Size: 816 2111 2898
Fragments arranged by size:
2898 2111 816
ApoI r'AATT_y
Cuts at: 2829 5178 5434 2829
Size: 2349 256 3220
Fragments arranged by size:
3220 2349 256
AscI GG'CGCG_CC
Cuts at: 140 140
Size: 5825
AseI AT'TA_AT
Cuts at: 20 20
Size: 5825
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5825
AvaI C'yCGr_G
Cuts at: 946 1092 1168 1245 1279 2250 2845 946
Size: 146 76 77 34 971 595 3926
Fragments arranged by size:
3926 971 595 146 77 76 34
AvrII C'CTAG_G
Cuts at: 1911 1911
Size: 5825
BamHI G'GATC_C
Cuts at: 2850 2850
Size: 5825
BanI G'GyrC_C
Cuts at: 1907 2374 2841 3255 3503 3538 1907
Size: 467 467 414 248 35 4194
Fragments arranged by size:
4194 467 467 414 248 35
BanII G_rGCy'C
Cuts at: 319 514 1099 1148 1891 2257 2839 3869 319
Size: 195 585 49 743 366 582 1030 2275
Fragments arranged by size:
2275 1030 743 585 582 366 195 49
BbeI G_GCGC'C
Cuts at: 2378 3259 3507 2378
Size: 881 248 4696
Fragments arranged by size:
4696 881 248
BbsI GAAGACnn'nnnn_
Cuts at: 252 659 1180 1537 252
Size: 407 521 357 4540
Fragments arranged by size:
4540 521 407 357
BbvCI CC'TCA_GC
Cuts at: 527 1042 1286 527
Size: 515 244 5066
Fragments arranged by size:
5066 515 244
BciVI GTATCCnnnnn_n'
Cuts at: 416 1715 3723 4484 5825 416
Size: 1299 2008 761 1341 416
Fragments arranged by size:
2008 1341 1299 761 416
BclI T'GATC_A
Cuts at: 3345 3345
Size: 5825
BglI GCCn_nnn'nGGC
Cuts at: 128 137 1403 2305 128
Size: 9 1266 902 3648
Fragments arranged by size:
3648 1266 902 9
BglII A'GATC_T
Cuts at: 611 3340 5377 611
Size: 2729 2037 1059
Fragments arranged by size:
2729 2037 1059
BlpI GC'TnA_GC
Cuts at: 565 2937 565
Size: 2372 3453
Bme1580I G_kGCm'C
Cuts at: 170 224 1169 1672 2061 2488 3450 3543 4599
Size: 54 945 503 389 427 962 93 1056
Cuts at: 4599 170
Size: 1396
Fragments arranged by size:
1396 1056 962 945 503 427 389 93
54
BmgBI CAC'GTC
Cuts at: 792 1164 2625 792
Size: 372 1461 3992
Fragments arranged by size:
3992 1461 372
BmrI ACTGGGnnnn_n'
Cuts at: 353 1894 3037 3118 3453 5597 353
Size: 1541 1143 81 335 2144 581
Fragments arranged by size:
2144 1541 1143 581 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 303 741 2229 4192 142
Size: 161 438 1488 1963 1775
Fragments arranged by size:
1963 1775 1488 438 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 292 1980 2002 2485 189
Size: 103 1688 22 483 3529
Fragments arranged by size:
3529 1688 483 103 22
Bpu10I CC'TnA_GC
Cuts at: 527 1042 1286 2137 527
Size: 515 244 851 4215
Fragments arranged by size:
4215 851 515 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3001 3880 4372 4670 2464
Size: 537 879 492 298 3619
Fragments arranged by size:
3619 879 537 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1958 5529 1958
Size: 3571 2254
BsaAI yAC'GTr
Cuts at: 1673 2772 3178 1673
Size: 1099 406 4320
Fragments arranged by size:
4320 1099 406
BsaBI GATnn'nnATC
Cuts at: 6 616 3339 5376 6
Size: 610 2723 2037 455
Fragments arranged by size:
2723 2037 610 455
BsaHI Gr'CG_yC
Cuts at: 752 835 1490 1637 1688 2375 2441 2732 3256
Size: 83 655 147 51 687 66 291 524
Cuts at: 3256 3504 4206 5702 752
Size: 248 702 1496 875
Fragments arranged by size:
1496 875 702 687 655 524 291 248
147 83 66 51
BsaWI w'CCGG_w
Cuts at: 2492 3014 3535 4213 4487 4634 5045 2492
Size: 522 521 678 274 147 411 3272
Fragments arranged by size:
3272 678 522 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1662 1692 1816 1846 1900 1930 1662
Size: 30 124 30 54 30 5557
Fragments arranged by size:
5557 124 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 717 721 724 970 2259 2316 717
Size: 4 3 246 1289 57 4226
Fragments arranged by size:
4226 1289 246 57 4 3
BseYI C'CCAG_C
Cuts at: 211 1000 1327 1748 2359 2518 3250 4008 4585
Size: 789 327 421 611 159 732 758 577
Cuts at: 4585 5081 211
Size: 496 955
Fragments arranged by size:
955 789 758 732 611 577 496 421
327 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 778 1531 2655 2745 252
Size: 526 753 1124 90 3332
Fragments arranged by size:
3332 1124 753 526 90
BsiEI CG_ry'CG
Cuts at: 104 608 3367 3413 4621 104
Size: 504 2759 46 1208 1308
Fragments arranged by size:
2759 1308 1208 504 46
BsiHKAI G_wGCw'C
Cuts at: 941 998 1099 1256 1414 1672 1678 2488 2839
Size: 57 101 157 158 258 6 810 351
Cuts at: 2839 3617 3807 4599 941
Size: 778 190 792 2167
Fragments arranged by size:
2167 810 792 778 351 258 190 158
157 101 57 6
BsmBI CGTCTCn'nnnn_
Cuts at: 1719 1719
Size: 5825
Bsp1286I G_dGCh'C
Cuts at: 170 224 319 514 941 998 1099 1148 1169
Size: 54 95 195 427 57 101 49 21
Cuts at: 1169 1256 1414 1672 1678 1891 2061 2257 2488
Size: 87 158 258 6 213 170 196 231
Cuts at: 2488 2839 3450 3543 3617 3807 3869 4599 170
Size: 351 611 93 74 190 62 730 1396
Fragments arranged by size:
1396 730 611 427 351 258 231 213
196 195 190 170 158 101 95 93
87 74 62 57 54 49 21 6
BspEI T'CCGG_A
Cuts at: 5045 5045
Size: 5825
BspHI T'CATG_A
Cuts at: 5001 5001
Size: 5825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2385 2465 2875 3089 3391 3772 2385
Size: 80 410 214 302 381 4438
Fragments arranged by size:
4438 410 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 1428 1455 2342 4117 4171 5655 5820 50
Size: 1378 27 887 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 1378 887 165 55 54 27
BsrDI GCAATG_nn'
Cuts at: 3737 3737
Size: 5825
BsrFI r'CCGG_y
Cuts at: 670 2660 3823 4004 4213 670
Size: 1990 1163 181 209 2282
Fragments arranged by size:
2282 1990 1163 209 181
BssHII G'CGCG_C
Cuts at: 140 149 140
Size: 9 5816
BssSI C'ACGA_G
Cuts at: 4096 4454 4096
Size: 358 5467
BstAPI GCAn_nnn'nTGC
Cuts at: 2293 2422 2694 3202 2293
Size: 129 272 508 4916
Fragments arranged by size:
4916 508 272 129
BstBI TT'CG_AA
Cuts at: 4186 4186
Size: 5825
BstEII G'GTnAC_C
Cuts at: 2565 2565
Size: 5825
BstXI CCAn_nnnn'nTGG
Cuts at: 522 1467 2562 522
Size: 945 1095 3785
Fragments arranged by size:
3785 1095 945
BstYI r'GATC_y
Cuts at: 1 611 1520 2850 3317 3340 3675 3921 4933
Size: 610 909 1330 467 23 335 246 1012
Cuts at: 4933 5019 5031 5377 1
Size: 86 12 346 449
Fragments arranged by size:
1330 1012 909 610 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1268 1268
Size: 5825
BtgI C'CryG_G
Cuts at: 107 290 506 689 1661 1775 1898 1919 2561
Size: 183 216 183 972 114 123 21 642
Cuts at: 2561 107
Size: 3371
Fragments arranged by size:
3371 972 642 216 183 183 123 114
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2025 2058 3775 3956 4119 2025
Size: 33 1717 181 163 3731
Fragments arranged by size:
3731 1717 181 163 33
BtsI GCAGTG_nn'
Cuts at: 2672 2764 3068 2672
Size: 92 304 5429
Fragments arranged by size:
5429 304 92
ClaI AT'CG_AT
Cuts at: 820 820
Size: 5825
DraI TTT'AAA
Cuts at: 2824 2824
Size: 5825
DraIII CAC_nnn'GTG
Cuts at: 1899 1899
Size: 5825
DrdI GACnn_nn'nnGTC
Cuts at: 1036 2461 3531 4389 1036
Size: 1425 1070 858 2472
Fragments arranged by size:
2472 1425 1070 858
EaeI y'GGCC_r
Cuts at: 110 503 555 605 1066 1395 1901 2216 2297
Size: 393 52 50 461 329 506 315 81
Cuts at: 2297 2750 3410 3584 3975 4002 5347 110
Size: 453 660 174 391 27 1345 588
Fragments arranged by size:
1345 660 588 506 461 453 393 391
329 315 174 81 52 50 27
EagI C'GGCC_G
Cuts at: 605 3410 605
Size: 2805 3020
EarI CTCTTCn'nnn_
Cuts at: 415 2098 2144 2428 3848 4058 415
Size: 1683 46 284 1420 210 2182
Fragments arranged by size:
2182 1683 1420 284 210 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4033 4343 4489 5635 4033
Size: 310 146 1146 4223
Fragments arranged by size:
4223 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 2837 1097
Size: 1740 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 265 292 424 569 1686 1980 2002 2485
Size: 76 27 132 145 1117 294 22 483
Cuts at: 2485 3650 4082 4829 5097 189
Size: 1165 432 747 268 917
Fragments arranged by size:
1165 1117 917 747 483 432 294 268
145 132 76 27 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1371 1371
Size: 5825
EcoO109I rG'GnC_Cy
Cuts at: 591 909 927 960 1950 2454 2703 2964 591
Size: 318 18 33 990 504 249 261 3452
Fragments arranged by size:
3452 990 504 318 261 249 33 18
EcoRI G'AATT_C
Cuts at: 2829 2829
Size: 5825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2832 3844 2832
Size: 1012 4813
FseI GG_CCGG'CC
Cuts at: 674 674
Size: 5825
FspI TGC'GCA
Cuts at: 3606 3606
Size: 5825
HaeII r_GCGC'y
Cuts at: 468 880 1130 1482 2378 3259 3507 4529 5508
Size: 412 250 352 896 881 248 1022 979
Cuts at: 5508 468
Size: 785
Fragments arranged by size:
1022 979 896 881 785 412 352 250
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 1198 1230 1816 1848 2005 2037 2137
Size: 32 481 32 586 32 157 32 100
Cuts at: 2137 2169 2614 2646 3667 3697 3699 3729 685
Size: 32 445 32 1021 30 2 30 2781
Fragments arranged by size:
2781 1021 586 481 445 157 100 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1602 2864 1602
Size: 1262 4563
HindIII A'AGCT_T
Cuts at: 436 436
Size: 5825
KasI G'GCGC_C
Cuts at: 2374 3255 3503 2374
Size: 881 248 4696
Fragments arranged by size:
4696 881 248
KpnI G_GTAC'C
Cuts at: 1911 2845 1911
Size: 934 4891
MluI A'CGCG_T
Cuts at: 5258 5258
Size: 5825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 926 1658 2175 4496 4680 926
Size: 732 517 2321 184 2071
Fragments arranged by size:
2321 2071 732 517 184
MscI TGG'CCA
Cuts at: 557 1903 3586 557
Size: 1346 1683 2796
Fragments arranged by size:
2796 1683 1346
MslI CAynn'nnrTG
Cuts at: 163 520 2554 2560 3814 3941 163
Size: 357 2034 6 1254 127 2047
Fragments arranged by size:
2047 2034 1254 357 127 6
MspA1I CmG'CkG
Cuts at: 215 508 813 1010 1331 1515 1830 2525 2748
Size: 293 305 197 321 184 315 695 223
Cuts at: 2748 2936 3250 3610 4623 4868 215
Size: 188 314 360 1013 245 1172
Fragments arranged by size:
1172 1013 695 360 321 315 314 305
293 245 223 197 188 184
NaeI GCC'GGC
Cuts at: 672 2662 4006 672
Size: 1990 1344 2491
Fragments arranged by size:
2491 1990 1344
NarI GG'CG_CC
Cuts at: 2375 3256 3504 2375
Size: 881 248 4696
Fragments arranged by size:
4696 881 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5825
NgoMIV G'CCGG_C
Cuts at: 670 2660 4004 670
Size: 1990 1344 2491
Fragments arranged by size:
2491 1990 1344
NspI r_CATG'y
Cuts at: 1866 2697 2878 3378 4285 1866
Size: 831 181 500 907 3406
Fragments arranged by size:
3406 907 831 500 181
PasI CC'CwG_GG
Cuts at: 1185 1185
Size: 5825
PciI A'CATG_T
Cuts at: 4281 4281
Size: 5825
PflMI CCAn_nnn'nTGG
Cuts at: 1899 1953 1899
Size: 54 5771
PfoI T'CCnGG_A
Cuts at: 911 1946 911
Size: 1035 4790
PmeI GTTT'AAAC
Cuts at: 2824 2824
Size: 5825
PmlI CAC'GTG
Cuts at: 1673 2772 1673
Size: 1099 4726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4964 4996 4964
Size: 32 5793
PpuMI rG'GwC_Cy
Cuts at: 909 927 960 1950 2454 2703 909
Size: 18 33 990 504 249 4031
Fragments arranged by size:
4031 990 504 249 33 18
PshAI GACnn'nnGTC
Cuts at: 2563 2563
Size: 5825
PspXI vC'TCGA_Gb
Cuts at: 1092 1092
Size: 5825
PstI C_TGCA'G
Cuts at: 455 577 1520 1646 2396 2538 2872 3557 455
Size: 122 943 126 750 142 334 685 2723
Fragments arranged by size:
2723 943 750 685 334 142 126 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5825
PvuII CAG'CTG
Cuts at: 215 1515 1830 2748 3250 3610 215
Size: 1300 315 918 502 360 2430
Fragments arranged by size:
2430 1300 918 502 360 315
RsrII CG'GwC_CG
Cuts at: 4020 4020
Size: 5825
SacI G_AGCT'C
Cuts at: 1099 2839 1099
Size: 1740 4085
SacII CC_GC'GG
Cuts at: 509 509
Size: 5825
SalI G'TCGA_C
Cuts at: 2862 2862
Size: 5825
SapI GCTCTTCn'nnn_
Cuts at: 3848 4058 3848
Size: 210 5615
SbfI CC_TGCA'GG
Cuts at: 2872 2872
Size: 5825
ScaI AGT'ACT
Cuts at: 5342 5342
Size: 5825
SexAI A'CCwGG_T
Cuts at: 1372 1372
Size: 5825
SfcI C'TryA_G
Cuts at: 33 451 573 1504 1516 1642 2392 2534 2868
Size: 418 122 931 12 126 750 142 334
Cuts at: 2868 3553 4546 4737 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 931 750 685 418 334 191
142 126 122 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 128 1403 2305 128
Size: 1275 902 3648
Fragments arranged by size:
3648 1275 902
SfoI GGC'GCC
Cuts at: 2376 3257 3505 2376
Size: 881 248 4696
Fragments arranged by size:
4696 881 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5825
SmaI CCC'GGG
Cuts at: 2847 2847
Size: 5825
SmlI C'TyrA_G
Cuts at: 1092 2479 2980 3895 4387 4649 1092
Size: 1387 501 915 492 262 2268
Fragments arranged by size:
2268 1387 915 501 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5825
SphI G_CATG'C
Cuts at: 2878 3378 2878
Size: 500 5325
StuI AGG'CCT
Cuts at: 1082 1291 2308 2348 1082
Size: 209 1017 40 4559
Fragments arranged by size:
4559 1017 209 40
StyI C'CwwG_G
Cuts at: 107 677 1911 2300 2570 2807 2959 3313 107
Size: 570 1234 389 270 237 152 354 2619
Fragments arranged by size:
2619 1234 570 389 354 270 237 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2328 3408 3541 4000 4209 2328
Size: 1080 133 459 209 3944
Fragments arranged by size:
3944 1080 459 209 133
TatI w'GTAC_w
Cuts at: 2036 5340 2036
Size: 3304 2521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1662 1694 5342 5374 1662
Size: 32 3648 32 2113
Fragments arranged by size:
3648 2113 32 32
Tth111I GACn'n_nGTC
Cuts at: 907 1036 1179 1210 1350 3622 907
Size: 129 143 31 140 2272 3110
Fragments arranged by size:
3110 2272 143 140 129 31
XbaI T'CTAG_A
Cuts at: 2856 2856
Size: 5825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 366 3320 192
Size: 174 2954 2697
Fragments arranged by size:
2954 2697 174
XhoI C'TCGA_G
Cuts at: 1092 1092
Size: 5825
XmaI C'CCGG_G
Cuts at: 2845 2845
Size: 5825
XmnI GAAnn'nnTTC
Cuts at: 178 2779 178
Size: 2601 3224
ZraI GAC'GTC
Cuts at: 836 1638 1689 2733 836
Size: 802 51 1044 3928
Fragments arranged by size:
3928 1044 802 51
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI ApaI BaeI BcgI BfrBI BmtI BsiWI
BsmI BsrGI BstZ17I CspCI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspOMI
PsrI SanDI SgrAI SnaBI SrfI SspI SwaI