Restriction map for
pF1KA0603
(Circular) (Six-base) MAPSORT of: pF1KA0603.seq Check: 2098 from: 1 to: 7010
pF1KA0603 7010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2274 2877 4048 2274
Size: 603 1171 5236
Fragments arranged by size:
5236 1171 603
Acc65I G'GTAC_C
Cuts at: 4026 4026
Size: 7010
AclI AA'CG_TT
Cuts at: 1981 6850 1981
Size: 4869 2141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1401 1889 2303 3424 3470 3652 4835 5267
Size: 563 488 414 1121 46 182 1183 432
Cuts at: 5267 5620 6282 838
Size: 353 662 1566
Fragments arranged by size:
1566 1183 1121 662 563 488 432 414
353 182 46
AflIII A'CryG_T
Cuts at: 1692 2030 4362 6162 6443 1692
Size: 338 2332 1800 281 2259
Fragments arranged by size:
2332 2259 1800 338 281
AhdI GACnn_n'nnGTC
Cuts at: 558 3255 558
Size: 2697 4313
AleI CACnn'nnGTG
Cuts at: 1069 3176 1069
Size: 2107 4903
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3005 3644 3005
Size: 639 6371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 124 156 124
Size: 32 6978
AlwNI CAG_nnn'CTG
Cuts at: 816 1330 2901 4315 5753 816
Size: 514 1571 1414 1438 2073
Fragments arranged by size:
2073 1571 1438 1414 514
ApaLI G'TGCA_C
Cuts at: 1696 5848 1696
Size: 4152 2858
ApoI r'AATT_y
Cuts at: 1248 2070 2265 2888 3205 4014 6363 6619 1248
Size: 822 195 623 317 809 2349 256 1639
Fragments arranged by size:
2349 1639 822 809 623 317 256 195
AseI AT'TA_AT
Cuts at: 20 20
Size: 7010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7010
AvaI C'yCGr_G
Cuts at: 160 190 313 344 411 556 886 983 4030
Size: 30 123 31 67 145 330 97 3047
Cuts at: 4030 160
Size: 3140
Fragments arranged by size:
3140 3047 330 145 123 97 67 31
30
BamHI G'GATC_C
Cuts at: 4035 4035
Size: 7010
BanI G'GyrC_C
Cuts at: 333 882 925 4026 4440 4688 4723 333
Size: 549 43 3101 414 248 35 2620
Fragments arranged by size:
3101 2620 549 414 248 43 35
BanII G_rGCy'C
Cuts at: 161 2091 2125 4024 5054 161
Size: 1930 34 1899 1030 2117
Fragments arranged by size:
2117 1930 1899 1030 34
BbeI G_GCGC'C
Cuts at: 337 4444 4692 337
Size: 4107 248 2655
Fragments arranged by size:
4107 2655 248
BbsI GAAGACnn'nnnn_
Cuts at: 1693 2025 2185 2194 1693
Size: 332 160 9 6509
Fragments arranged by size:
6509 332 160 9
BbvCI CC'TCA_GC
Cuts at: 1968 2113 2472 1968
Size: 145 359 6506
Fragments arranged by size:
6506 359 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1024 1058 1024
Size: 34 6976
BciVI GTATCCnnnnn_n'
Cuts at: 3121 4908 5964 7010 3121
Size: 1787 1056 1046 3121
Fragments arranged by size:
3121 1787 1056 1046
BclI T'GATC_A
Cuts at: 533 4530 533
Size: 3997 3013
BglII A'GATC_T
Cuts at: 3312 4525 6562 3312
Size: 1213 2037 3760
Fragments arranged by size:
3760 2037 1213
BlpI GC'TnA_GC
Cuts at: 179 364 2993 4122 179
Size: 185 2629 1129 3067
Fragments arranged by size:
3067 2629 1129 185
Bme1580I G_kGCm'C
Cuts at: 421 887 1700 2028 4635 4728 5852 421
Size: 466 813 328 2607 93 1124 1579
Fragments arranged by size:
2607 1579 1124 813 466 328 93
BmgBI CAC'GTC
Cuts at: 1143 1143
Size: 7010
BmrI ACTGGGnnnn_n'
Cuts at: 1130 3344 4222 4303 4638 6782 1130
Size: 2214 878 81 335 2144 1358
Fragments arranged by size:
2214 2144 1358 878 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 5377 2734
Size: 2643 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1307 2109 2172 2704 3176 3736 175
Size: 1132 802 63 532 472 560 3449
Fragments arranged by size:
3449 1132 802 560 532 472 63
Bpu10I CC'TnA_GC
Cuts at: 998 1968 2090 2113 2472 998
Size: 970 122 23 359 5536
Fragments arranged by size:
5536 970 359 122 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 1818 4186 5065 5779 6077 747
Size: 1071 2368 879 714 298 1680
Fragments arranged by size:
2368 1680 1071 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1052 1522 3826 3950 6714 1052
Size: 470 2304 124 2764 1348
Fragments arranged by size:
2764 2304 1348 470 124
BsaAI yAC'GTr
Cuts at: 1695 4363 1695
Size: 2668 4342
BsaBI GATnn'nnATC
Cuts at: 6 4524 6561 6
Size: 4518 2037 455
Fragments arranged by size:
4518 2037 455
BsaHI Gr'CG_yC
Cuts at: 170 334 689 4441 4689 5391 6887 170
Size: 164 355 3752 248 702 1496 293
Fragments arranged by size:
3752 1496 702 355 293 248 164
BsaWI w'CCGG_w
Cuts at: 843 3854 3905 4199 4720 5398 5809 5956 6230
Size: 3011 51 294 521 678 411 147 274
Cuts at: 6230 843
Size: 1623
Fragments arranged by size:
3011 1623 678 521 411 294 274 147
51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1030 1060 1030
Size: 30 6980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 928 1254 2396 2560 2640 2897 928
Size: 326 1142 164 80 257 5041
Fragments arranged by size:
5041 1142 326 257 164 80
BseYI C'CCAG_C
Cuts at: 118 403 598 670 1106 4435 5193 5858 6266
Size: 285 195 72 436 3329 758 665 408
Cuts at: 6266 118
Size: 862
Fragments arranged by size:
3329 862 758 665 436 408 285 195
72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1074 2157 3154 784
Size: 290 1083 997 4640
Fragments arranged by size:
4640 1083 997 290
BsiEI CG_ry'CG
Cuts at: 104 4552 4598 5828 104
Size: 4448 46 1230 1286
Fragments arranged by size:
4448 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 365 470 1700 2125 4024 4802 4992 5852 365
Size: 105 1230 425 1899 778 190 860 1523
Fragments arranged by size:
1899 1523 1230 860 778 425 190 105
BsmI GAATG_Cn'
Cuts at: 125 125
Size: 7010
BsmBI CGTCTCn'nnnn_
Cuts at: 177 177
Size: 7010
Bsp1286I G_dGCh'C
Cuts at: 161 365 421 470 887 1700 2028 2091 2125
Size: 204 56 49 417 813 328 63 34
Cuts at: 2125 4024 4635 4728 4802 4992 5054 5852 161
Size: 1899 611 93 74 190 62 798 1319
Fragments arranged by size:
1899 1319 813 798 611 417 328 204
190 93 74 63 62 56 49 34
BspEI T'CCGG_A
Cuts at: 843 3854 3905 843
Size: 3011 51 3948
Fragments arranged by size:
3948 3011 51
BspHI T'CATG_A
Cuts at: 3297 5442 3297
Size: 2145 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 957 2371 4060 4274 4576 4957 957
Size: 1414 1689 214 302 381 3010
Fragments arranged by size:
3010 1689 1414 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1002 5302 5356 6840 7005 50
Size: 952 4300 54 1484 165 55
Fragments arranged by size:
4300 1484 952 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2376 2483 4922 2376
Size: 107 2439 4464
Fragments arranged by size:
4464 2439 107
BsrFI r'CCGG_y
Cuts at: 336 1066 5008 5189 336
Size: 730 3942 181 2157
Fragments arranged by size:
3942 2157 730 181
BsrGI T'GTAC_A
Cuts at: 2165 2165
Size: 7010
BssHII G'CGCG_C
Cuts at: 368 368
Size: 7010
BssSI C'ACGA_G
Cuts at: 1087 3010 5281 5989 1087
Size: 1923 2271 708 2108
Fragments arranged by size:
2271 2108 1923 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1488 4387 1488
Size: 2899 4111
BstBI TT'CG_AA
Cuts at: 713 2016 2670 5371 713
Size: 1303 654 2701 2352
Fragments arranged by size:
2701 2352 1303 654
BstEII G'GTnAC_C
Cuts at: 735 735
Size: 7010
BstYI r'GATC_y
Cuts at: 1 290 821 1801 2414 3312 4035 4502 4525
Size: 289 531 980 613 898 723 467 23
Cuts at: 4525 4860 5106 5412 5424 5510 6562 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 980 898 723 613 531 467 449
335 306 289 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1660 2440 1660
Size: 780 6230
BtgI C'CryG_G
Cuts at: 107 2540 3920 107
Size: 2433 1380 3197
Fragments arranged by size:
3197 2433 1380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4960 5141 5304 4960
Size: 181 163 6666
Fragments arranged by size:
6666 181 163
BtsI GCAGTG_nn'
Cuts at: 1065 1764 2231 2312 2987 4253 1065
Size: 699 467 81 675 1266 3822
Fragments arranged by size:
3822 1266 699 675 467 81
ClaI AT'CG_AT
Cuts at: 773 773
Size: 7010
DraI TTT'AAA
Cuts at: 1254 4009 1254
Size: 2755 4255
DrdI GACnn_nn'nnGTC
Cuts at: 4716 6060 4716
Size: 1344 5666
EaeI y'GGCC_r
Cuts at: 284 431 647 4595 4769 5160 5187 6532 284
Size: 147 216 3948 174 391 27 1345 762
Fragments arranged by size:
3948 1345 762 391 216 174 147 27
EagI C'GGCC_G
Cuts at: 4595 4595
Size: 7010
EarI CTCTTCn'nnn_
Cuts at: 1186 1985 2855 3176 5033 5243 1186
Size: 799 870 321 1857 210 2953
Fragments arranged by size:
2953 1857 870 799 321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5218 5960 6106 6820 5218
Size: 742 146 714 5408
Fragments arranged by size:
5408 742 714 146
EcoICRI GAG'CTC
Cuts at: 2123 4022 2123
Size: 1899 5111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 838 1307 1401 1889 2109 2172 2303 2704
Size: 663 469 94 488 220 63 131 401
Cuts at: 2704 3176 3424 3470 3652 3736 4835 5267 5620
Size: 472 248 46 182 84 1099 432 353
Cuts at: 5620 6282 175
Size: 662 903
Fragments arranged by size:
1099 903 663 662 488 472 469 432
401 353 248 220 182 131 94 84
63 46
EcoNI CCTnn'n_nnAGG
Cuts at: 535 910 535
Size: 375 6635
EcoO109I rG'GnC_Cy
Cuts at: 407 756 858 890 1711 2351 4149 407
Size: 349 102 32 821 640 1798 3268
Fragments arranged by size:
3268 1798 821 640 349 102 32
EcoRI G'AATT_C
Cuts at: 2265 4014 2265
Size: 1749 5261
EcoRV GAT'ATC
Cuts at: 1260 1260
Size: 7010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1821 3514 5029 1821
Size: 1693 1515 3802
Fragments arranged by size:
3802 1693 1515
FspI TGC'GCA
Cuts at: 4791 4791
Size: 7010
HaeII r_GCGC'y
Cuts at: 337 404 816 4444 4692 5922 6693 337
Size: 67 412 3628 248 1230 771 654
Fragments arranged by size:
3628 1230 771 654 412 248 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 370 544 576 966 998 1030 1062 1213
Size: 32 174 32 390 32 32 32 151
Cuts at: 1213 1245 1249 1281 1500 1532 1987 2019 2804
Size: 32 4 32 219 32 455 32 785
Cuts at: 2804 2836 3241 3273 4852 4882 4884 4914 338
Size: 32 405 32 1579 30 2 30 2434
Fragments arranged by size:
2434 1579 785 455 405 390 219 174
151 32 32 32 32 32 32 32
32 32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 2878 4049 2878
Size: 1171 5839
HindIII A'AGCT_T
Cuts at: 2253 2253
Size: 7010
KasI G'GCGC_C
Cuts at: 333 4440 4688 333
Size: 4107 248 2655
Fragments arranged by size:
4107 2655 248
KpnI G_GTAC'C
Cuts at: 4030 4030
Size: 7010
MfeI C'AATT_G
Cuts at: 3936 3936
Size: 7010
MluI A'CGCG_T
Cuts at: 6443 6443
Size: 7010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2024 2560 5769 5953 1916
Size: 108 536 3209 184 2973
Fragments arranged by size:
3209 2973 536 184 108
MscI TGG'CCA
Cuts at: 4771 4771
Size: 7010
MslI CAynn'nnrTG
Cuts at: 1069 3176 3632 4999 5126 1069
Size: 2107 456 1367 127 2953
Fragments arranged by size:
2953 2107 1367 456 127
MspA1I CmG'CkG
Cuts at: 122 1668 2556 3075 3282 3922 4121 4435 4795
Size: 1546 888 519 207 640 199 314 360
Cuts at: 4795 5579 5824 122
Size: 784 245 1308
Fragments arranged by size:
1546 1308 888 784 640 519 360 314
245 207 199
NaeI GCC'GGC
Cuts at: 338 5191 338
Size: 4853 2157
NarI GG'CG_CC
Cuts at: 334 4441 4689 334
Size: 4107 248 2655
Fragments arranged by size:
4107 2655 248
NcoI C'CATG_G
Cuts at: 107 2540 107
Size: 2433 4577
NgoMIV G'CCGG_C
Cuts at: 336 5189 336
Size: 4853 2157
NspI r_CATG'y
Cuts at: 1291 2166 2765 3004 3631 4063 4563 6166 1291
Size: 875 599 239 627 432 500 1603 2135
Fragments arranged by size:
2135 1603 875 627 599 500 432 239
PciI A'CATG_T
Cuts at: 6162 6162
Size: 7010
PflMI CCAn_nnn'nTGG
Cuts at: 1330 3798 1330
Size: 2468 4542
PmeI GTTT'AAAC
Cuts at: 4009 4009
Size: 7010
PmlI CAC'GTG
Cuts at: 1695 1695
Size: 7010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 5456
Size: 32 6978
PpuMI rG'GwC_Cy
Cuts at: 858 890 2351 858
Size: 32 1461 5517
Fragments arranged by size:
5517 1461 32
PshAI GACnn'nnGTC
Cuts at: 3075 3075
Size: 7010
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 7010
PstI C_TGCA'G
Cuts at: 1439 2368 2812 3266 4057 4742 1439
Size: 929 444 454 791 685 3707
Fragments arranged by size:
3707 929 791 685 454 444
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7010
PvuII CAG'CTG
Cuts at: 122 1668 3075 3282 4435 4795 122
Size: 1546 1407 207 1153 360 2337
Fragments arranged by size:
2337 1546 1407 1153 360 207
RsrII CG'GwC_CG
Cuts at: 841 5205 841
Size: 4364 2646
SacI G_AGCT'C
Cuts at: 2125 4024 2125
Size: 1899 5111
SacII CC_GC'GG
Cuts at: 3923 3923
Size: 7010
SalI G'TCGA_C
Cuts at: 2876 4047 2876
Size: 1171 5839
SanDI GG'GwC_CC
Cuts at: 890 890
Size: 7010
SapI GCTCTTCn'nnn_
Cuts at: 1186 3176 5033 5243 1186
Size: 1990 1857 210 2953
Fragments arranged by size:
2953 1990 1857 210
SbfI CC_TGCA'GG
Cuts at: 4057 4057
Size: 7010
ScaI AGT'ACT
Cuts at: 6527 6527
Size: 7010
SfcI C'TryA_G
Cuts at: 33 1435 2364 2808 3262 3655 4053 4738 5706
Size: 1402 929 444 454 393 398 685 968
Cuts at: 5706 5897 33
Size: 191 1146
Fragments arranged by size:
1402 1146 968 929 685 454 444 398
393 191
SfoI GGC'GCC
Cuts at: 335 4442 4690 335
Size: 4107 248 2655
Fragments arranged by size:
4107 2655 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7010
SgrAI Cr'CCGG_yG
Cuts at: 336 1066 336
Size: 730 6280
SmaI CCC'GGG
Cuts at: 192 888 4032 192
Size: 696 3144 3170
Fragments arranged by size:
3170 3144 696
SmlI C'TyrA_G
Cuts at: 762 1833 4165 5080 5794 6056 762
Size: 1071 2332 915 714 262 1716
Fragments arranged by size:
2332 1716 1071 915 714 262
SpeI A'CTAG_T
Cuts at: 67 421 67
Size: 354 6656
SphI G_CATG'C
Cuts at: 3004 3631 4063 4563 3004
Size: 627 432 500 5451
Fragments arranged by size:
5451 627 500 432
SspI AAT'ATT
Cuts at: 3528 3528
Size: 7010
StuI AGG'CCT
Cuts at: 1414 1471 3796 1414
Size: 57 2325 4628
Fragments arranged by size:
4628 2325 57
StyI C'CwwG_G
Cuts at: 107 825 939 2540 3797 4144 4498 107
Size: 718 114 1601 1257 347 354 2619
Fragments arranged by size:
2619 1601 1257 718 354 347 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 1034 1149 2057 4593 4726 5185 5394 949
Size: 85 115 908 2536 133 459 209 2565
Fragments arranged by size:
2565 2536 908 459 209 133 115 85
TatI w'GTAC_w
Cuts at: 722 1732 2165 3054 3239 6525 722
Size: 1010 433 889 185 3286 1207
Fragments arranged by size:
3286 1207 1010 889 433 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6527 6559 6527
Size: 32 6978
Tth111I GACn'n_nGTC
Cuts at: 4807 4807
Size: 7010
XbaI T'CTAG_A
Cuts at: 4041 4041
Size: 7010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1377 4505 1377
Size: 3128 3882
XmaI C'CCGG_G
Cuts at: 190 886 4030 190
Size: 696 3144 3170
Fragments arranged by size:
3170 3144 696
XmnI GAAnn'nnTTC
Cuts at: 1601 2840 2864 1601
Size: 1239 24 5747
Fragments arranged by size:
5747 1239 24
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BstXI BstZ17I CspCI
DraIII FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PspOMI PspXI PsrI SexAI
SfiI SnaBI SrfI SwaI XhoI ZraI