Restriction map for
pF1KA0606
(Circular) (Six-base) MAPSORT of: pF1KA0606.seq Check: 8424 from: 1 to: 6728
pF1KA0606 6728 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3326 3326
Size: 6728
AccI GT'mk_AC
Cuts at: 3766 3766
Size: 6728
Acc65I G'GTAC_C
Cuts at: 3744 3744
Size: 6728
AclI AA'CG_TT
Cuts at: 6568 6568
Size: 6728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1699 2129 2282 2539 3307 4553 4985 5732 6000
Size: 430 153 257 768 1246 432 747 268
Cuts at: 6000 1699
Size: 2427
Fragments arranged by size:
2427 1246 768 747 432 430 268 257
153
AfeI AGC'GCT
Cuts at: 1199 2820 3115 1199
Size: 1621 295 4812
Fragments arranged by size:
4812 1621 295
AflII C'TTAA_G
Cuts at: 444 444
Size: 6728
AflIII A'CryG_T
Cuts at: 731 1002 4080 5184 6161 731
Size: 271 3078 1104 977 1298
Fragments arranged by size:
3078 1298 1104 977 271
AgeI A'CCGG_T
Cuts at: 5116 5116
Size: 6728
AhdI GACnn_n'nnGTC
Cuts at: 253 2709 253
Size: 2456 4272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 763 1840 763
Size: 1077 5651
AlwNI CAG_nnn'CTG
Cuts at: 2858 3318 4033 5600 2858
Size: 460 715 1567 3986
Fragments arranged by size:
3986 1567 715 460
ApaLI G'TGCA_C
Cuts at: 2238 5498 2238
Size: 3260 3468
ApoI r'AATT_y
Cuts at: 883 3732 6081 6337 883
Size: 2849 2349 256 1274
Fragments arranged by size:
2849 2349 1274 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
AvaI C'yCGr_G
Cuts at: 3748 3748
Size: 6728
AvrII C'CTAG_G
Cuts at: 2682 2682
Size: 6728
BamHI G'GATC_C
Cuts at: 2397 2407 3546 3753 2397
Size: 10 1139 207 5372
Fragments arranged by size:
5372 1139 207 10
BanI G'GyrC_C
Cuts at: 2220 2359 2625 3327 3428 3744 4158 4406 4441
Size: 139 266 702 101 316 414 248 35
Cuts at: 4441 2220
Size: 4507
Fragments arranged by size:
4507 702 414 316 266 248 139 101
35
BanII G_rGCy'C
Cuts at: 1502 2082 2873 3014 3065 3078 3227 3742 4772
Size: 580 791 141 51 13 149 515 1030
Cuts at: 4772 1502
Size: 3458
Fragments arranged by size:
3458 1030 791 580 515 149 141 51
13
BbeI G_GCGC'C
Cuts at: 3432 4162 4410 3432
Size: 730 248 5750
Fragments arranged by size:
5750 730 248
BbsI GAAGACnn'nnnn_
Cuts at: 3231 3231
Size: 6728
BbvCI CC'TCA_GC
Cuts at: 3653 3653
Size: 6728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2132 2166 2132
Size: 34 6694
BciVI GTATCCnnnnn_n'
Cuts at: 4626 5387 6728 4626
Size: 761 1341 4626
Fragments arranged by size:
4626 1341 761
BclI T'GATC_A
Cuts at: 2001 2049 2936 3555 4248 2001
Size: 48 887 619 693 4481
Fragments arranged by size:
4481 887 693 619 48
BfrBI ATG'CAT
Cuts at: 318 318
Size: 6728
BglI GCCn_nnn'nGGC
Cuts at: 1443 2884 1443
Size: 1441 5287
BglII A'GATC_T
Cuts at: 2488 4243 6280 2488
Size: 1755 2037 2936
Fragments arranged by size:
2936 2037 1755
BlpI GC'TnA_GC
Cuts at: 2872 3539 3840 2872
Size: 667 301 5760
Fragments arranged by size:
5760 667 301
Bme1580I G_kGCm'C
Cuts at: 1661 2225 2242 2750 4353 4446 5502 1661
Size: 564 17 508 1603 93 1056 2887
Fragments arranged by size:
2887 1603 1056 564 508 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 1237 2612 2894 3098 3940 4021 4356 6500 1237
Size: 1375 282 204 842 81 335 2144 1465
Fragments arranged by size:
2144 1465 1375 842 335 282 204 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5095 5095
Size: 6728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1768 2218 2961 3536 3643 1562
Size: 206 450 743 575 107 4647
Fragments arranged by size:
4647 743 575 450 206 107
Bpu10I CC'TnA_GC
Cuts at: 1906 3079 3653 1906
Size: 1173 574 4981
Fragments arranged by size:
4981 1173 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1516 3904 4783 5275 5573 1516
Size: 2388 879 492 298 2671
Fragments arranged by size:
2671 2388 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 952 2198 6432 952
Size: 1246 4234 1248
Fragments arranged by size:
4234 1248 1246
BsaAI yAC'GTr
Cuts at: 2640 4081 2640
Size: 1441 5287
BsaBI GATnn'nnATC
Cuts at: 6 4242 6279 6
Size: 4236 2037 455
Fragments arranged by size:
4236 2037 455
BsaHI Gr'CG_yC
Cuts at: 249 3236 3429 4159 4407 5109 6605 249
Size: 2987 193 730 248 702 1496 372
Fragments arranged by size:
2987 1496 730 702 372 248 193
BsaWI w'CCGG_w
Cuts at: 307 1397 2074 2207 3917 4438 5116 5390 5537
Size: 1090 677 133 1710 521 678 274 147
Cuts at: 5537 5948 307
Size: 411 1087
Fragments arranged by size:
1710 1090 1087 678 677 521 411 274
147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2085 2115 2085
Size: 30 6698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 378 2734 3235 3601 378
Size: 2356 501 366 3505
Fragments arranged by size:
3505 2356 501 366
BseYI C'CCAG_C
Cuts at: 479 2082 2341 2961 4153 4911 5488 5984 479
Size: 1603 259 620 1192 758 577 496 1223
Fragments arranged by size:
1603 1223 1192 758 620 577 496 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2662 2851 2662
Size: 189 6539
BsiEI CG_ry'CG
Cuts at: 104 4270 4316 5524 104
Size: 4166 46 1208 1308
Fragments arranged by size:
4166 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1502 2242 2654 2873 3014 3742 4520 4710 5502
Size: 740 412 219 141 728 778 190 792
Cuts at: 5502 1502
Size: 2728
Fragments arranged by size:
2728 792 778 740 728 412 219 190
141
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 6728
Bsp1286I G_dGCh'C
Cuts at: 1502 1661 2082 2225 2242 2654 2750 2873 3014
Size: 159 421 143 17 412 96 123 141
Cuts at: 3014 3065 3078 3227 3742 4353 4446 4520 4710
Size: 51 13 149 515 611 93 74 190
Cuts at: 4710 4772 5502 1502
Size: 62 730 2728
Fragments arranged by size:
2728 730 611 515 421 412 190 159
149 143 141 123 96 93 74 62
51 17 13
BspEI T'CCGG_A
Cuts at: 1397 2074 5948 1397
Size: 677 3874 2177
Fragments arranged by size:
3874 2177 677
BspHI T'CATG_A
Cuts at: 1808 2498 3602 5904 1808
Size: 690 1104 2302 2632
Fragments arranged by size:
2632 2302 1104 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 280 1955 3326 3778 3992 4294 4675 280
Size: 1675 1371 452 214 302 381 2333
Fragments arranged by size:
2333 1675 1371 452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5020 5074 6558 6723 50
Size: 4970 54 1484 165 55
Fragments arranged by size:
4970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 747 4640 747
Size: 3893 2835
BsrFI r'CCGG_y
Cuts at: 2878 4726 4907 5116 2878
Size: 1848 181 209 4490
Fragments arranged by size:
4490 1848 209 181
BssSI C'ACGA_G
Cuts at: 504 4999 5357 504
Size: 4495 358 1875
Fragments arranged by size:
4495 1875 358
BstAPI GCAn_nnn'nTGC
Cuts at: 324 2649 3318 4105 324
Size: 2325 669 787 2947
Fragments arranged by size:
2947 2325 787 669
BstBI TT'CG_AA
Cuts at: 3507 5089 3507
Size: 1582 5146
BstXI CCAn_nnnn'nTGG
Cuts at: 2090 2090
Size: 6728
BstYI r'GATC_y
Cuts at: 1 1008 1688 2397 2407 2488 3546 3753 4220
Size: 1007 680 709 10 81 1058 207 467
Cuts at: 4220 4243 4578 4824 5836 5922 5934 6280 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1058 1012 1007 709 680 467 449 346
335 246 207 86 81 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 586 964 586
Size: 378 6350
BtgI C'CryG_G
Cuts at: 107 2726 3673 107
Size: 2619 947 3162
Fragments arranged by size:
3162 2619 947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4678 4859 5022 4678
Size: 181 163 6384
Fragments arranged by size:
6384 181 163
BtsI GCAGTG_nn'
Cuts at: 3012 3279 3971 3012
Size: 267 692 5769
Fragments arranged by size:
5769 692 267
DraI TTT'AAA
Cuts at: 572 3727 572
Size: 3155 3573
DraIII CAC_nnn'GTG
Cuts at: 1833 3477 1833
Size: 1644 5084
DrdI GACnn_nn'nnGTC
Cuts at: 4434 5292 4434
Size: 858 5870
EaeI y'GGCC_r
Cuts at: 1344 4313 4487 4878 4905 6250 1344
Size: 2969 174 391 27 1345 1822
Fragments arranged by size:
2969 1822 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4313 4313
Size: 6728
EarI CTCTTCn'nnn_
Cuts at: 544 646 1215 1589 2258 2507 2515 3509 3578
Size: 102 569 374 669 249 8 994 69
Cuts at: 3578 4751 4961 544
Size: 1173 210 2311
Fragments arranged by size:
2311 1173 994 669 569 374 249 210
102 69 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4936 5246 5392 6538 4936
Size: 310 146 1146 5126
Fragments arranged by size:
5126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1500 2871 3012 3740 1500
Size: 1371 141 728 4488
Fragments arranged by size:
4488 1371 728 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1699 1768 2129 2218 2282 2539 2961 3307
Size: 137 69 361 89 64 257 422 346
Cuts at: 3307 3536 3643 4553 4985 5732 6000 1562
Size: 229 107 910 432 747 268 2290
Fragments arranged by size:
2290 910 747 432 422 361 346 268
257 229 137 107 89 69 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 3322 2060
Size: 1262 5466
EcoO109I rG'GnC_Cy
Cuts at: 269 3867 269
Size: 3598 3130
EcoRI G'AATT_C
Cuts at: 3732 3732
Size: 6728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 425 3420 4747 425
Size: 2995 1327 2406
Fragments arranged by size:
2995 2406 1327
FspI TGC'GCA
Cuts at: 2740 4509 2740
Size: 1769 4959
HaeII r_GCGC'y
Cuts at: 1201 2822 2879 3117 3159 3173 3188 3368 3432
Size: 1621 57 238 42 14 15 180 64
Cuts at: 3432 4162 4410 5432 6411 1201
Size: 730 248 1022 979 1518
Fragments arranged by size:
1621 1518 1022 979 730 248 238 180
64 57 42 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 239 271 2480 2512 2695 2727 2928 2935 2960
Size: 32 2209 32 183 32 201 7 25
Cuts at: 2960 2967 3028 3060 4570 4600 4602 4632 239
Size: 7 61 32 1510 30 2 30 2335
Fragments arranged by size:
2335 2209 1510 201 183 61 32 32
32 32 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 1667 3047 3767 1667
Size: 1380 720 4628
Fragments arranged by size:
4628 1380 720
HindIII A'AGCT_T
Cuts at: 1096 2111 2350 1096
Size: 1015 239 5474
Fragments arranged by size:
5474 1015 239
HpaI GTT'AAC
Cuts at: 1667 1667
Size: 6728
KasI G'GCGC_C
Cuts at: 3428 4158 4406 3428
Size: 730 248 5750
Fragments arranged by size:
5750 730 248
KpnI G_GTAC'C
Cuts at: 3748 3748
Size: 6728
MluI A'CGCG_T
Cuts at: 6161 6161
Size: 6728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 736 750 782 818 1871 3167 3218 5399 5583
Size: 14 32 36 1053 1296 51 2181 184
Cuts at: 5583 736
Size: 1881
Fragments arranged by size:
2181 1881 1296 1053 184 51 36 32
14
MscI TGG'CCA
Cuts at: 1346 4489 1346
Size: 3143 3585
MslI CAynn'nnrTG
Cuts at: 2317 2618 4717 4844 2317
Size: 301 2099 127 4201
Fragments arranged by size:
4201 2099 301 127
MspA1I CmG'CkG
Cuts at: 610 2036 2086 2365 2475 2984 3669 3675 3687
Size: 1426 50 279 110 509 685 6 12
Cuts at: 3687 3696 3839 4153 4513 5526 5771 610
Size: 9 143 314 360 1013 245 1567
Fragments arranged by size:
1567 1426 1013 685 509 360 314 279
245 143 110 50 12 9 6
NaeI GCC'GGC
Cuts at: 4909 4909
Size: 6728
NarI GG'CG_CC
Cuts at: 3429 4159 4407 3429
Size: 730 248 5750
Fragments arranged by size:
5750 730 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6728
NgoMIV G'CCGG_C
Cuts at: 4907 4907
Size: 6728
NsiI A_TGCA'T
Cuts at: 320 320
Size: 6728
NspI r_CATG'y
Cuts at: 322 1006 1069 1827 3123 3781 4281 5188 322
Size: 684 63 758 1296 658 500 907 1862
Fragments arranged by size:
1862 1296 907 758 684 658 500 63
PciI A'CATG_T
Cuts at: 1002 5184 1002
Size: 4182 2546
PflMI CCAn_nnn'nTGG
Cuts at: 334 334
Size: 6728
PfoI T'CCnGG_A
Cuts at: 2400 2400
Size: 6728
PmeI GTTT'AAAC
Cuts at: 3727 3727
Size: 6728
PmlI CAC'GTG
Cuts at: 2640 2640
Size: 6728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5867 5899 5867
Size: 32 6696
PpuMI rG'GwC_Cy
Cuts at: 269 269
Size: 6728
PstI C_TGCA'G
Cuts at: 706 1964 3286 3775 4460 706
Size: 1258 1322 489 685 2974
Fragments arranged by size:
2974 1322 1258 685 489
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6728
PvuII CAG'CTG
Cuts at: 610 2086 3669 3687 3696 4153 4513 610
Size: 1476 1583 18 9 457 360 2825
Fragments arranged by size:
2825 1583 1476 457 360 18 9
RsrII CG'GwC_CG
Cuts at: 4923 4923
Size: 6728
SacI G_AGCT'C
Cuts at: 1502 2873 3014 3742 1502
Size: 1371 141 728 4488
Fragments arranged by size:
4488 1371 728 141
SacII CC_GC'GG
Cuts at: 3676 3676
Size: 6728
SalI G'TCGA_C
Cuts at: 3765 3765
Size: 6728
SapI GCTCTTCn'nnn_
Cuts at: 2258 2507 4751 4961 2258
Size: 249 2244 210 4025
Fragments arranged by size:
4025 2244 249 210
SbfI CC_TGCA'GG
Cuts at: 3775 3775
Size: 6728
ScaI AGT'ACT
Cuts at: 6245 6245
Size: 6728
SfcI C'TryA_G
Cuts at: 33 543 702 970 1060 1960 2060 3282 3491
Size: 510 159 268 90 900 100 1222 209
Cuts at: 3491 3771 4456 5449 5640 33
Size: 280 685 993 191 1121
Fragments arranged by size:
1222 1121 993 900 685 510 280 268
209 191 159 100 90
SfoI GGC'GCC
Cuts at: 3430 4160 4408 3430
Size: 730 248 5750
Fragments arranged by size:
5750 730 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
SgrAI Cr'CCGG_yG
Cuts at: 2878 2878
Size: 6728
SmaI CCC'GGG
Cuts at: 3750 3750
Size: 6728
SmlI C'TyrA_G
Cuts at: 444 1495 3883 4798 5290 5552 444
Size: 1051 2388 915 492 262 1620
Fragments arranged by size:
2388 1620 1051 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3720 67
Size: 3653 3075
SphI G_CATG'C
Cuts at: 1827 3123 3781 4281 1827
Size: 1296 658 500 4274
Fragments arranged by size:
4274 1296 658 500
StyI C'CwwG_G
Cuts at: 107 467 2682 3293 3862 4216 107
Size: 360 2215 611 569 354 2619
Fragments arranged by size:
2619 2215 611 569 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2913 4311 4444 4903 5112 2913
Size: 1398 133 459 209 4529
Fragments arranged by size:
4529 1398 459 209 133
TatI w'GTAC_w
Cuts at: 6243 6243
Size: 6728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 938 970 2629 2661 6245 6277 490
Size: 32 416 32 1659 32 3584 32 941
Fragments arranged by size:
3584 1659 941 416 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2580 4525 2580
Size: 1945 4783
XbaI T'CTAG_A
Cuts at: 1107 3759 1107
Size: 2652 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 236 2592 2985 4223 236
Size: 2356 393 1238 2741
Fragments arranged by size:
2741 2356 1238 393
XmaI C'CCGG_G
Cuts at: 3748 3748
Size: 6728
XmnI GAAnn'nnTTC
Cuts at: 179 1223 1571 2161 179
Size: 1044 348 590 4746
Fragments arranged by size:
4746 1044 590 348
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AleI AloI ApaI AscI BaeI BmgBI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
PacI PasI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI