Restriction map for
pF1KA0609
(Circular) (Six-base) MAPSORT of: pF1KA0609.seq Check: 1036 from: 1 to: 5225
pF1KA0609 5225 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1373 1373
Size: 5225
AccI GT'mk_AC
Cuts at: 2263 2263
Size: 5225
Acc65I G'GTAC_C
Cuts at: 935 1829 2241 935
Size: 894 412 3919
Fragments arranged by size:
3919 894 412
AclI AA'CG_TT
Cuts at: 5065 5065
Size: 5225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 3050 3482 4229 4497 746
Size: 2304 432 747 268 1474
Fragments arranged by size:
2304 1474 747 432 268
AfeI AGC'GCT
Cuts at: 1300 1300
Size: 5225
AflIII A'CryG_T
Cuts at: 1223 1625 2162 2577 3681 4658 1223
Size: 402 537 415 1104 977 1790
Fragments arranged by size:
1790 1104 977 537 415 402
AgeI A'CCGG_T
Cuts at: 3613 3613
Size: 5225
AleI CACnn'nnGTG
Cuts at: 1000 1000
Size: 5225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1552 1584 2017 2049 1552
Size: 32 433 32 4728
Fragments arranged by size:
4728 433 32 32
AlwNI CAG_nnn'CTG
Cuts at: 651 1413 2530 4097 651
Size: 762 1117 1567 1779
Fragments arranged by size:
1779 1567 1117 762
ApaI G_GGCC'C
Cuts at: 1445 1445
Size: 5225
ApaLI G'TGCA_C
Cuts at: 3995 3995
Size: 5225
ApoI r'AATT_y
Cuts at: 136 2229 4578 4834 136
Size: 2093 2349 256 527
Fragments arranged by size:
2349 2093 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
AvaI C'yCGr_G
Cuts at: 2245 2245
Size: 5225
BamHI G'GATC_C
Cuts at: 737 2250 737
Size: 1513 3712
BanI G'GyrC_C
Cuts at: 678 935 1829 2241 2655 2903 2938 678
Size: 257 894 412 414 248 35 2965
Fragments arranged by size:
2965 894 414 412 257 248 35
BanII G_rGCy'C
Cuts at: 378 719 1079 1445 1510 1667 2239 3269 378
Size: 341 360 366 65 157 572 1030 2334
Fragments arranged by size:
2334 1030 572 366 360 341 157 65
BbeI G_GCGC'C
Cuts at: 2659 2907 2659
Size: 248 4977
BbsI GAAGACnn'nnnn_
Cuts at: 151 151
Size: 5225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5191
BciVI GTATCCnnnnn_n'
Cuts at: 538 3123 3884 5225 538
Size: 2585 761 1341 538
Fragments arranged by size:
2585 1341 761 538
BclI T'GATC_A
Cuts at: 2745 2745
Size: 5225
BglI GCCn_nnn'nGGC
Cuts at: 2177 2177
Size: 5225
BglII A'GATC_T
Cuts at: 2740 4777 2740
Size: 2037 3188
BlpI GC'TnA_GC
Cuts at: 355 1385 2337 355
Size: 1030 952 3243
Fragments arranged by size:
3243 1030 952
Bme1580I G_kGCm'C
Cuts at: 430 683 1445 2850 2943 3999 430
Size: 253 762 1405 93 1056 1656
Fragments arranged by size:
1656 1405 1056 762 253 93
BmgBI CAC'GTC
Cuts at: 1224 1836 1945 1224
Size: 612 109 4504
Fragments arranged by size:
4504 612 109
BmrI ACTGGGnnnn_n'
Cuts at: 1443 2437 2518 2853 4997 1443
Size: 994 81 335 2144 1671
Fragments arranged by size:
2144 1671 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 5225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 262 322 452 832 1225 1390 1435 261
Size: 1 60 130 380 393 165 45 4051
Fragments arranged by size:
4051 393 380 165 130 60 45 1
Bpu10I CC'TnA_GC
Cuts at: 1101 1101
Size: 5225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 2125 2401 3280 3772 4070 703
Size: 1422 276 879 492 298 1858
Fragments arranged by size:
1858 1422 879 492 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 4929 4929
Size: 5225
BsaAI yAC'GTr
Cuts at: 2578 2578
Size: 5225
BsaBI GATnn'nnATC
Cuts at: 6 2739 4776 6
Size: 2733 2037 455
Fragments arranged by size:
2733 2037 455
BsaHI Gr'CG_yC
Cuts at: 1370 1472 2656 2904 3606 5102 1370
Size: 102 1184 248 702 1496 1493
Fragments arranged by size:
1496 1493 1184 702 248 102
BsaWI w'CCGG_w
Cuts at: 1964 2414 2935 3613 3887 4034 4445 1964
Size: 450 521 678 274 147 411 2744
Fragments arranged by size:
2744 678 521 450 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 1036 1066 1186 1216 1552 1582 1777
Size: 30 747 30 120 30 336 30 195
Cuts at: 1777 1807 2017 2047 259
Size: 30 210 30 3437
Fragments arranged by size:
3437 747 336 210 195 120 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 343 436 340
Size: 3 93 5129
Fragments arranged by size:
5129 93 3
BseYI C'CCAG_C
Cuts at: 111 171 1481 2080 2650 3408 3985 4481 111
Size: 60 1310 599 570 758 577 496 855
Fragments arranged by size:
1310 855 758 599 577 570 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1201 1318 2041 595
Size: 606 117 723 3779
Fragments arranged by size:
3779 723 606 117
BsiEI CG_ry'CG
Cuts at: 104 500 1965 2767 2813 4021 104
Size: 396 1465 802 46 1208 1308
Fragments arranged by size:
1465 1308 1208 802 396 46
BsiHKAI G_wGCw'C
Cuts at: 719 1079 2239 3017 3207 3999 719
Size: 360 1160 778 190 792 1945
Fragments arranged by size:
1945 1160 792 778 360 190
BsmBI CGTCTCn'nnnn_
Cuts at: 123 1214 1935 123
Size: 1091 721 3413
Fragments arranged by size:
3413 1091 721
Bsp1286I G_dGCh'C
Cuts at: 378 430 683 719 1079 1445 1510 1667 2239
Size: 52 253 36 360 366 65 157 572
Cuts at: 2239 2850 2943 3017 3207 3269 3999 378
Size: 611 93 74 190 62 730 1604
Fragments arranged by size:
1604 730 611 572 366 360 253 190
157 93 74 65 62 52 36
BspEI T'CCGG_A
Cuts at: 4445 4445
Size: 5225
BspHI T'CATG_A
Cuts at: 4401 4401
Size: 5225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1592 2275 2489 2791 3172 1592
Size: 683 214 302 381 3645
Fragments arranged by size:
3645 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1202 3517 3571 5055 5220 50
Size: 1152 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 1152 165 55 54
BsrDI GCAATG_nn'
Cuts at: 635 857 1730 3137 635
Size: 222 873 1407 2723
Fragments arranged by size:
2723 1407 873 222
BsrFI r'CCGG_y
Cuts at: 495 1757 3223 3404 3613 495
Size: 1262 1466 181 209 2107
Fragments arranged by size:
2107 1466 1262 209 181
BsrGI T'GTAC_A
Cuts at: 1553 1553
Size: 5225
BssSI C'ACGA_G
Cuts at: 3496 3854 3496
Size: 358 4867
BstAPI GCAn_nnn'nTGC
Cuts at: 2602 2602
Size: 5225
BstBI TT'CG_AA
Cuts at: 209 3586 209
Size: 3377 1848
BstXI CCAn_nnnn'nTGG
Cuts at: 1452 1452
Size: 5225
BstYI r'GATC_y
Cuts at: 1 647 737 2250 2717 2740 3075 3321 4333
Size: 646 90 1513 467 23 335 246 1012
Cuts at: 4333 4419 4431 4777 1
Size: 86 12 346 449
Fragments arranged by size:
1513 1012 646 467 449 346 335 246
90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1678 1678
Size: 5225
BtgI C'CryG_G
Cuts at: 416 967 1394 416
Size: 551 427 4247
Fragments arranged by size:
4247 551 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 370 3175 3356 3519 370
Size: 2805 181 163 2076
Fragments arranged by size:
2805 2076 181 163
BtsI GCAGTG_nn'
Cuts at: 1216 1747 2468 1216
Size: 531 721 3973
Fragments arranged by size:
3973 721 531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 840 875 840
Size: 35 5190
DraI TTT'AAA
Cuts at: 2224 2224
Size: 5225
DraIII CAC_nnn'GTG
Cuts at: 1536 1551 1536
Size: 15 5210
DrdI GACnn_nn'nnGTC
Cuts at: 2931 3789 2931
Size: 858 4367
EaeI y'GGCC_r
Cuts at: 653 970 1598 2810 2984 3375 3402 4747 653
Size: 317 628 1212 174 391 27 1345 1131
Fragments arranged by size:
1345 1212 1131 628 391 317 174 27
EagI C'GGCC_G
Cuts at: 2810 2810
Size: 5225
EarI CTCTTCn'nnn_
Cuts at: 667 803 978 1822 2141 3248 3458 667
Size: 136 175 844 319 1107 210 2434
Fragments arranged by size:
2434 1107 844 319 210 175 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1893 3433 3743 3889 5035 1893
Size: 1540 310 146 1146 2083
Fragments arranged by size:
2083 1540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 717 1077 2237 717
Size: 360 1160 3705
Fragments arranged by size:
3705 1160 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 262 322 452 746 832 1225 1390 1435
Size: 1 60 130 294 86 393 165 45
Cuts at: 1435 3050 3482 4229 4497 261
Size: 1615 432 747 268 989
Fragments arranged by size:
1615 989 747 432 393 294 268 165
130 86 60 45 1
EcoNI CCTnn'n_nnAGG
Cuts at: 364 364
Size: 5225
EcoO109I rG'GnC_Cy
Cuts at: 1441 1811 2364 1441
Size: 370 553 4302
Fragments arranged by size:
4302 553 370
EcoRI G'AATT_C
Cuts at: 2229 2229
Size: 5225
EcoRV GAT'ATC
Cuts at: 840 840
Size: 5225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 5225
FspI TGC'GCA
Cuts at: 310 1591 3006 310
Size: 1281 1415 2529
Fragments arranged by size:
2529 1415 1281
FspAI rTGC'GCAy
Cuts at: 310 310
Size: 5225
HaeII r_GCGC'y
Cuts at: 1302 2659 2907 3929 4908 1302
Size: 1357 248 1022 979 1619
Fragments arranged by size:
1619 1357 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 753 780 785 809 812 841 1777
Size: 32 15 27 5 24 3 29 936
Cuts at: 1777 1809 3067 3097 3099 3129 706
Size: 32 1258 30 2 30 2802
Fragments arranged by size:
2802 1258 936 32 32 30 30 29
27 24 15 5 3 2
HincII GTy'rAC
Cuts at: 2264 2264
Size: 5225
HindIII A'AGCT_T
Cuts at: 778 778
Size: 5225
KasI G'GCGC_C
Cuts at: 2655 2903 2655
Size: 248 4977
KpnI G_GTAC'C
Cuts at: 939 1833 2245 939
Size: 894 412 3919
Fragments arranged by size:
3919 894 412
MluI A'CGCG_T
Cuts at: 4658 4658
Size: 5225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2132 3896 4080 2132
Size: 1764 184 3277
Fragments arranged by size:
3277 1764 184
MscI TGG'CCA
Cuts at: 655 972 1600 2986 655
Size: 317 628 1386 2894
Fragments arranged by size:
2894 1386 628 317
MslI CAynn'nnrTG
Cuts at: 1000 1084 3214 3341 1000
Size: 84 2130 127 2884
Fragments arranged by size:
2884 2130 127 84
MspA1I CmG'CkG
Cuts at: 240 285 685 1185 1251 1389 2336 2650 3010
Size: 45 400 500 66 138 947 314 360
Cuts at: 3010 4023 4268 240
Size: 1013 245 1197
Fragments arranged by size:
1197 1013 947 500 400 360 314 245
138 66 45
NaeI GCC'GGC
Cuts at: 3406 3406
Size: 5225
NarI GG'CG_CC
Cuts at: 2656 2904 2656
Size: 248 4977
NcoI C'CATG_G
Cuts at: 416 967 1394 416
Size: 551 427 4247
Fragments arranged by size:
4247 551 427
NgoMIV G'CCGG_C
Cuts at: 3404 3404
Size: 5225
NspI r_CATG'y
Cuts at: 315 1089 1629 2070 2166 2278 2778 3685 315
Size: 774 540 441 96 112 500 907 1855
Fragments arranged by size:
1855 907 774 540 500 441 112 96
PasI CC'CwG_GG
Cuts at: 366 366
Size: 5225
PciI A'CATG_T
Cuts at: 1625 2162 3681 1625
Size: 537 1519 3169
Fragments arranged by size:
3169 1519 537
PflMI CCAn_nnn'nTGG
Cuts at: 182 979 1413 1536 182
Size: 797 434 123 3871
Fragments arranged by size:
3871 797 434 123
PfoI T'CCnGG_A
Cuts at: 733 733
Size: 5225
PmeI GTTT'AAAC
Cuts at: 2224 2224
Size: 5225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1552 1584 2017 2049 4364 4396 1552
Size: 32 433 32 2315 32 2381
Fragments arranged by size:
2381 2315 433 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1811 1811
Size: 5225
PspOMI G'GGCC_C
Cuts at: 1441 1441
Size: 5225
PstI C_TGCA'G
Cuts at: 124 1356 2272 2957 124
Size: 1232 916 685 2392
Fragments arranged by size:
2392 1232 916 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5225
PvuII CAG'CTG
Cuts at: 1185 1251 1389 2650 3010 1185
Size: 66 138 1261 360 3400
Fragments arranged by size:
3400 1261 360 138 66
RsrII CG'GwC_CG
Cuts at: 1880 3420 1880
Size: 1540 3685
SacI G_AGCT'C
Cuts at: 719 1079 2239 719
Size: 360 1160 3705
Fragments arranged by size:
3705 1160 360
SalI G'TCGA_C
Cuts at: 2262 2262
Size: 5225
SanDI GG'GwC_CC
Cuts at: 1811 1811
Size: 5225
SapI GCTCTTCn'nnn_
Cuts at: 667 3248 3458 667
Size: 2581 210 2434
Fragments arranged by size:
2581 2434 210
SbfI CC_TGCA'GG
Cuts at: 2272 2272
Size: 5225
ScaI AGT'ACT
Cuts at: 4742 4742
Size: 5225
SfcI C'TryA_G
Cuts at: 33 120 1215 1352 2268 2953 3946 4137 33
Size: 87 1095 137 916 685 993 191 1121
Fragments arranged by size:
1121 1095 993 916 685 191 137 87
SfoI GGC'GCC
Cuts at: 2657 2905 2657
Size: 248 4977
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
SmaI CCC'GGG
Cuts at: 2247 2247
Size: 5225
SmlI C'TyrA_G
Cuts at: 718 2140 2380 3295 3787 4049 718
Size: 1422 240 915 492 262 1894
Fragments arranged by size:
1894 1422 915 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5225
SphI G_CATG'C
Cuts at: 315 1089 2278 2778 315
Size: 774 1189 500 2762
Fragments arranged by size:
2762 1189 774 500
StyI C'CwwG_G
Cuts at: 416 939 967 978 1394 2359 2713 416
Size: 523 28 11 416 965 354 2928
Fragments arranged by size:
2928 965 523 416 354 28 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1333 1872 2808 2941 3400 3609 1333
Size: 539 936 133 459 209 2949
Fragments arranged by size:
2949 936 539 459 209 133
TatI w'GTAC_w
Cuts at: 1325 1553 4740 1325
Size: 228 3187 1810
Fragments arranged by size:
3187 1810 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 209 241 1036 1068 1186 1218 1261 1293 4742
Size: 32 795 32 118 32 43 32 3449
Cuts at: 4742 4774 209
Size: 32 660
Fragments arranged by size:
3449 795 660 118 43 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3022 3022
Size: 5225
XbaI T'CTAG_A
Cuts at: 2256 2256
Size: 5225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 179 2720 179
Size: 2541 2684
XmaI C'CCGG_G
Cuts at: 2245 2245
Size: 5225
XmnI GAAnn'nnTTC
Cuts at: 2150 2150
Size: 5225
ZraI GAC'GTC
Cuts at: 1371 1371
Size: 5225
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AscI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I ClaI
FseI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI