Restriction map for
pF1KA0610
(Circular) (Six-base) MAPSORT of: pF1KA0610.seq Check: 811 from: 1 to: 5111
pF1KA0610 5111 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 272 582 272
Size: 310 4801
AccI GT'mk_AC
Cuts at: 2149 2149
Size: 5111
Acc65I G'GTAC_C
Cuts at: 845 2127 845
Size: 1282 3829
AclI AA'CG_TT
Cuts at: 4951 4951
Size: 5111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 545 665 898 2936 3368 4115 4383 412
Size: 133 120 233 2038 432 747 268 1140
Fragments arranged by size:
2038 1140 747 432 268 233 133 120
AflIII A'CryG_T
Cuts at: 470 986 1641 2463 3567 4544 470
Size: 516 655 822 1104 977 1037
Fragments arranged by size:
1104 1037 977 822 655 516
AgeI A'CCGG_T
Cuts at: 3499 3499
Size: 5111
AleI CACnn'nnGTG
Cuts at: 1210 1210
Size: 5111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1035 1806 1035
Size: 771 4340
AlwNI CAG_nnn'CTG
Cuts at: 313 568 679 1587 1819 2416 3983 313
Size: 255 111 908 232 597 1567 1441
Fragments arranged by size:
1567 1441 908 597 255 232 111
ApaLI G'TGCA_C
Cuts at: 827 3881 827
Size: 3054 2057
ApoI r'AATT_y
Cuts at: 386 454 1147 2115 4464 4720 386
Size: 68 693 968 2349 256 777
Fragments arranged by size:
2349 968 777 693 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 5111
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5111
AvaI C'yCGr_G
Cuts at: 904 2131 904
Size: 1227 3884
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 820 853 1914 1947 820
Size: 33 1061 33 3984
Fragments arranged by size:
3984 1061 33 33
BamHI G'GATC_C
Cuts at: 2136 2136
Size: 5111
BanI G'GyrC_C
Cuts at: 435 845 2127 2541 2789 2824 435
Size: 410 1282 414 248 35 2722
Fragments arranged by size:
2722 1282 414 410 248 35
BanII G_rGCy'C
Cuts at: 1073 2125 3155 1073
Size: 1052 1030 3029
Fragments arranged by size:
3029 1052 1030
BbeI G_GCGC'C
Cuts at: 2545 2793 2545
Size: 248 4863
BbvCI CC'TCA_GC
Cuts at: 515 515
Size: 5111
BciVI GTATCCnnnnn_n'
Cuts at: 672 848 3009 3770 5111 672
Size: 176 2161 761 1341 672
Fragments arranged by size:
2161 1341 761 672 176
BclI T'GATC_A
Cuts at: 2631 2631
Size: 5111
BfrBI ATG'CAT
Cuts at: 1793 1793
Size: 5111
BglII A'GATC_T
Cuts at: 952 2626 4663 952
Size: 1674 2037 1400
Fragments arranged by size:
2037 1674 1400
BlpI GC'TnA_GC
Cuts at: 2223 2223
Size: 5111
Bme1580I G_kGCm'C
Cuts at: 440 831 2736 2829 3885 440
Size: 391 1905 93 1056 1666
Fragments arranged by size:
1905 1666 1056 391 93
BmrI ACTGGGnnnn_n'
Cuts at: 2323 2404 2739 4883 2323
Size: 81 335 2144 2551
Fragments arranged by size:
2551 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 5111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1096
Size: 5111
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 5111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 760 2002 2287 3166 3658 3956 620
Size: 140 1242 285 879 492 298 1775
Fragments arranged by size:
1775 1242 879 492 298 285 140
BsaI GGTCTCn'nnnn_
Cuts at: 736 4815 736
Size: 4079 1032
BsaAI yAC'GTr
Cuts at: 2464 2464
Size: 5111
BsaBI GATnn'nnATC
Cuts at: 6 2625 4662 6
Size: 2619 2037 455
Fragments arranged by size:
2619 2037 455
BsaHI Gr'CG_yC
Cuts at: 2542 2790 3492 4988 2542
Size: 248 702 1496 2665
Fragments arranged by size:
2665 1496 702 248
BsaWI w'CCGG_w
Cuts at: 957 2300 2821 3499 3773 3920 4331 957
Size: 1343 521 678 274 147 411 1737
Fragments arranged by size:
1737 1343 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 531 561 1961 1991 531
Size: 30 1400 30 3651
Fragments arranged by size:
3651 1400 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 503 608 623 718 1167 1577 1697 503
Size: 105 15 95 449 410 120 3917
Fragments arranged by size:
3917 449 410 120 105 95 15
BseYI C'CCAG_C
Cuts at: 2536 3294 3871 4367 2536
Size: 758 577 496 3280
Fragments arranged by size:
3280 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 575 1802 554
Size: 21 1227 3863
Fragments arranged by size:
3863 1227 21
BsiEI CG_ry'CG
Cuts at: 104 2653 2699 3907 104
Size: 2549 46 1208 1308
Fragments arranged by size:
2549 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 831 1073 2125 2903 3093 3885 831
Size: 242 1052 778 190 792 2057
Fragments arranged by size:
2057 1052 792 778 242 190
Bsp1286I G_dGCh'C
Cuts at: 440 831 1073 2125 2736 2829 2903 3093 3155
Size: 391 242 1052 611 93 74 190 62
Cuts at: 3155 3885 440
Size: 730 1666
Fragments arranged by size:
1666 1052 730 611 391 242 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4331 4331
Size: 5111
BspHI T'CATG_A
Cuts at: 4287 4287
Size: 5111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 144 272 582 2161 2375 2677 3058 144
Size: 128 310 1579 214 302 381 2197
Fragments arranged by size:
2197 1579 381 310 302 214 128
BsrBI CCG'CTC
Cuts at: 50 1482 3403 3457 4941 5106 50
Size: 1432 1921 54 1484 165 55
Fragments arranged by size:
1921 1484 1432 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1946 3023 1946
Size: 1077 4034
BsrFI r'CCGG_y
Cuts at: 3109 3290 3499 3109
Size: 181 209 4721
Fragments arranged by size:
4721 209 181
BsrGI T'GTAC_A
Cuts at: 1821 1821
Size: 5111
BssSI C'ACGA_G
Cuts at: 3382 3740 3382
Size: 358 4753
BstAPI GCAn_nnn'nTGC
Cuts at: 568 2488 568
Size: 1920 3191
BstBI TT'CG_AA
Cuts at: 852 3472 852
Size: 2620 2491
BstXI CCAn_nnnn'nTGG
Cuts at: 1159 1159
Size: 5111
BstYI r'GATC_y
Cuts at: 1 952 1080 2136 2603 2626 2961 3207 4219
Size: 951 128 1056 467 23 335 246 1012
Cuts at: 4219 4305 4317 4663 1
Size: 86 12 346 449
Fragments arranged by size:
1056 1012 951 467 449 346 335 246
128 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 747 747
Size: 5111
BtgI C'CryG_G
Cuts at: 107 1505 107
Size: 1398 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3061 3242 3405 3061
Size: 181 163 4767
Fragments arranged by size:
4767 181 163
BtsI GCAGTG_nn'
Cuts at: 2354 2354
Size: 5111
DraI TTT'AAA
Cuts at: 2110 2110
Size: 5111
DrdI GACnn_nn'nnGTC
Cuts at: 2817 3675 2817
Size: 858 4253
EaeI y'GGCC_r
Cuts at: 2696 2870 3261 3288 4633 2696
Size: 174 391 27 1345 3174
Fragments arranged by size:
3174 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2696 2696
Size: 5111
EarI CTCTTCn'nnn_
Cuts at: 3134 3344 3134
Size: 210 4901
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3319 3629 3775 4921 3319
Size: 310 146 1146 3509
Fragments arranged by size:
3509 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1071 2123 1071
Size: 1052 4059
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 545 665 898 1096 2936 3368 4115 4383
Size: 133 120 233 198 1840 432 747 268
Cuts at: 4383 412
Size: 1140
Fragments arranged by size:
1840 1140 747 432 268 233 198 133
120
EcoO109I rG'GnC_Cy
Cuts at: 310 2250 310
Size: 1940 3171
EcoRI G'AATT_C
Cuts at: 1147 2115 1147
Size: 968 4143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 192 3130 192
Size: 2938 2173
FspI TGC'GCA
Cuts at: 2892 2892
Size: 5111
HaeII r_GCGC'y
Cuts at: 2545 2793 3815 4794 2545
Size: 248 1022 979 2862
Fragments arranged by size:
2862 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 481 513 676 708 1687 1719 1961
Size: 32 198 32 163 32 979 32 242
Cuts at: 1961 1993 2004 2036 2953 2983 2985 3015 251
Size: 32 11 32 917 30 2 30 2347
Fragments arranged by size:
2347 979 917 242 198 163 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 189 1299 1800 2043 2150 189
Size: 1110 501 243 107 3150
Fragments arranged by size:
3150 1110 501 243 107
HpaI GTT'AAC
Cuts at: 189 1299 1800 189
Size: 1110 501 3500
Fragments arranged by size:
3500 1110 501
KasI G'GCGC_C
Cuts at: 2541 2789 2541
Size: 248 4863
KpnI G_GTAC'C
Cuts at: 849 2131 849
Size: 1282 3829
MluI A'CGCG_T
Cuts at: 4544 4544
Size: 5111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 678 1202 1602 3782 3966 678
Size: 524 400 2180 184 1823
Fragments arranged by size:
2180 1823 524 400 184
MscI TGG'CCA
Cuts at: 2872 2872
Size: 5111
MslI CAynn'nnrTG
Cuts at: 1210 3100 3227 1210
Size: 1890 127 3094
Fragments arranged by size:
3094 1890 127
MspA1I CmG'CkG
Cuts at: 1522 2222 2536 2896 3909 4154 1522
Size: 700 314 360 1013 245 2479
Fragments arranged by size:
2479 1013 700 360 314 245
NaeI GCC'GGC
Cuts at: 3292 3292
Size: 5111
NarI GG'CG_CC
Cuts at: 2542 2790 2542
Size: 248 4863
NcoI C'CATG_G
Cuts at: 107 107
Size: 5111
NgoMIV G'CCGG_C
Cuts at: 3290 3290
Size: 5111
NsiI A_TGCA'T
Cuts at: 1795 1795
Size: 5111
NspI r_CATG'y
Cuts at: 474 523 990 1645 2164 2664 3571 474
Size: 49 467 655 519 500 907 2014
Fragments arranged by size:
2014 907 655 519 500 467 49
PciI A'CATG_T
Cuts at: 470 986 1641 3567 470
Size: 516 655 1926 2014
Fragments arranged by size:
2014 1926 655 516
PflMI CCAn_nnn'nTGG
Cuts at: 127 1878 127
Size: 1751 3360
PmeI GTTT'AAAC
Cuts at: 2110 2110
Size: 5111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 5079
PpuMI rG'GwC_Cy
Cuts at: 310 310
Size: 5111
PstI C_TGCA'G
Cuts at: 419 816 2158 2843 419
Size: 397 1342 685 2687
Fragments arranged by size:
2687 1342 685 397
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5111
PvuII CAG'CTG
Cuts at: 1522 2536 2896 1522
Size: 1014 360 3737
Fragments arranged by size:
3737 1014 360
RsrII CG'GwC_CG
Cuts at: 3306 3306
Size: 5111
SacI G_AGCT'C
Cuts at: 1073 2125 1073
Size: 1052 4059
SalI G'TCGA_C
Cuts at: 2148 2148
Size: 5111
SapI GCTCTTCn'nnn_
Cuts at: 3134 3344 3134
Size: 210 4901
SbfI CC_TGCA'GG
Cuts at: 816 2158 816
Size: 1342 3769
ScaI AGT'ACT
Cuts at: 4628 4628
Size: 5111
SfcI C'TryA_G
Cuts at: 33 361 415 812 1556 1607 2154 2839 3832
Size: 328 54 397 744 51 547 685 993
Cuts at: 3832 4023 33
Size: 191 1121
Fragments arranged by size:
1121 993 744 685 547 397 328 191
54 51
SfoI GGC'GCC
Cuts at: 2543 2791 2543
Size: 248 4863
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5111
SmaI CCC'GGG
Cuts at: 906 2133 906
Size: 1227 3884
SmlI C'TyrA_G
Cuts at: 635 739 1981 2266 3181 3673 3935 635
Size: 104 1242 285 915 492 262 1811
Fragments arranged by size:
1811 1242 915 492 285 262 104
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5111
SphI G_CATG'C
Cuts at: 523 2164 2664 523
Size: 1641 500 2970
Fragments arranged by size:
2970 1641 500
SspI AAT'ATT
Cuts at: 1917 1917
Size: 5111
StuI AGG'CCT
Cuts at: 175 175
Size: 5111
StyI C'CwwG_G
Cuts at: 107 1227 1529 2245 2599 107
Size: 1120 302 716 354 2619
Fragments arranged by size:
2619 1120 716 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 305 1395 2694 2827 3286 3495 305
Size: 1090 1299 133 459 209 1921
Fragments arranged by size:
1921 1299 1090 459 209 133
TatI w'GTAC_w
Cuts at: 789 970 1821 4626 789
Size: 181 851 2805 1274
Fragments arranged by size:
2805 1274 851 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 531 563 1010 1042 4628 4660 531
Size: 32 447 32 3586 32 982
Fragments arranged by size:
3586 982 447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2908 2908
Size: 5111
XbaI T'CTAG_A
Cuts at: 390 2142 390
Size: 1752 3359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2606 2606
Size: 5111
XmaI C'CCGG_G
Cuts at: 904 2131 904
Size: 1227 3884
XmnI GAAnn'nnTTC
Cuts at: 675 675
Size: 5111
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbsI BcgI BglI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI