Restriction map for
pF1KA0612
(Circular) (Six-base) MAPSORT of: pF1KA0612.seq Check: 4355 from: 1 to: 8504
pF1KA0612 8504 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1110 2700 3066 1110
Size: 1590 366 6548
Fragments arranged by size:
6548 1590 366
AccI GT'mk_AC
Cuts at: 1402 5542 1402
Size: 4140 4364
Acc65I G'GTAC_C
Cuts at: 5520 5520
Size: 8504
AclI AA'CG_TT
Cuts at: 8344 8344
Size: 8504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 457 613 1261 1379 3221 4927 6329 6761
Size: 98 156 648 118 1842 1706 1402 432
Cuts at: 6761 7114 7776 359
Size: 353 662 1087
Fragments arranged by size:
1842 1706 1402 1087 662 648 432 353
156 118 98
AfeI AGC'GCT
Cuts at: 514 514
Size: 8504
AflII C'TTAA_G
Cuts at: 526 526
Size: 8504
AflIII A'CryG_T
Cuts at: 5856 7656 7937 5856
Size: 1800 281 6423
Fragments arranged by size:
6423 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 194 3600 4842 194
Size: 3406 1242 3856
Fragments arranged by size:
3856 3406 1242
AleI CACnn'nnGTG
Cuts at: 2632 2632
Size: 8504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 533 2840 533
Size: 2307 6197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2141
Size: 32 8472
AlwNI CAG_nnn'CTG
Cuts at: 546 945 1158 1206 1278 1395 2146 2430 2511
Size: 399 213 48 72 117 751 284 81
Cuts at: 2511 3072 3253 3604 3876 4484 4522 4905 5031
Size: 561 181 351 272 608 38 383 126
Cuts at: 5031 5809 7247 546
Size: 778 1438 1803
Fragments arranged by size:
1803 1438 778 751 608 561 399 383
351 284 272 213 181 126 117 81
72 48 38
ApaI G_GGCC'C
Cuts at: 495 648 820 861 1519 2489 2789 2900 3558
Size: 153 172 41 658 970 300 111 658
Cuts at: 3558 4238 4261 4300 4342 4711 5413 495
Size: 680 23 39 42 369 702 3586
Fragments arranged by size:
3586 970 702 680 658 658 369 300
172 153 111 42 41 39 23
ApaLI G'TGCA_C
Cuts at: 7342 7342
Size: 8504
ApoI r'AATT_y
Cuts at: 5508 7857 8113 5508
Size: 2349 256 5899
Fragments arranged by size:
5899 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8504
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8504
AvaI C'yCGr_G
Cuts at: 813 853 861 1424 1512 2564 3182 3989 4655
Size: 40 8 563 88 1052 618 807 666
Cuts at: 4655 5524 813
Size: 869 3793
Fragments arranged by size:
3793 1052 869 807 666 618 563 88
40 8
BamHI G'GATC_C
Cuts at: 1889 5529 1889
Size: 3640 4864
BanI G'GyrC_C
Cuts at: 843 2442 2560 2577 2634 3412 3564 5520 5934
Size: 1599 118 17 57 778 152 1956 414
Cuts at: 5934 6182 6217 843
Size: 248 35 3130
Fragments arranged by size:
3130 1956 1599 778 414 248 152 118
57 35 17
BanII G_rGCy'C
Cuts at: 313 495 648 820 861 959 1439 1493 1519
Size: 182 153 172 41 98 480 54 26
Cuts at: 1519 1648 2033 2255 2266 2489 2789 2900 3558
Size: 129 385 222 11 223 300 111 658
Cuts at: 3558 4159 4238 4261 4300 4342 4711 4774 5077
Size: 601 79 23 39 42 369 63 303
Cuts at: 5077 5413 5518 6548 313
Size: 336 105 1030 2269
Fragments arranged by size:
2269 1030 658 601 480 385 369 336
303 300 223 222 182 172 153 129
111 105 98 79 63 54 42 41
39 26 23 11
BbeI G_GCGC'C
Cuts at: 847 2446 3568 5938 6186 847
Size: 1599 1122 2370 248 3165
Fragments arranged by size:
3165 2370 1599 1122 248
BbsI GAAGACnn'nnnn_
Cuts at: 1664 1890 4059 4115 1664
Size: 226 2169 56 6053
Fragments arranged by size:
6053 2169 226 56
BbvCI CC'TCA_GC
Cuts at: 487 547 1144 3610 4994 487
Size: 60 597 2466 1384 3997
Fragments arranged by size:
3997 2466 1384 597 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2275 2309 3064 3098 3173 3207 3490 3524 2275
Size: 34 755 34 75 34 283 34 7255
Fragments arranged by size:
7255 755 283 75 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6402 7458 8504 6402
Size: 1056 1046 6402
Fragments arranged by size:
6402 1056 1046
BclI T'GATC_A
Cuts at: 2915 6024 2915
Size: 3109 5395
BglI GCCn_nnn'nGGC
Cuts at: 177 613 807 1726 2048 3526 3562 4337 4539
Size: 436 194 919 322 1478 36 775 202
Cuts at: 4539 4967 177
Size: 428 3714
Fragments arranged by size:
3714 1478 919 775 436 428 322 202
194 36
BglII A'GATC_T
Cuts at: 3860 6019 8056 3860
Size: 2159 2037 4308
Fragments arranged by size:
4308 2159 2037
BlpI GC'TnA_GC
Cuts at: 958 1306 2768 5616 958
Size: 348 1462 2848 3846
Fragments arranged by size:
3846 2848 1462 348
Bme1580I G_kGCm'C
Cuts at: 168 495 648 820 861 1519 2489 2565 2582
Size: 327 153 172 41 658 970 76 17
Cuts at: 2582 2639 2689 2789 2900 3415 3558 4238 4261
Size: 57 50 100 111 515 143 680 23
Cuts at: 4261 4300 4342 4516 4711 5413 6129 6222 7346
Size: 39 42 174 195 702 716 93 1124
Cuts at: 7346 168
Size: 1326
Fragments arranged by size:
1326 1124 970 716 702 680 658 515
327 195 174 172 153 143 111 100
93 76 57 50 42 41 39 23
17
BmgBI CAC'GTC
Cuts at: 5283 5283
Size: 8504
BmrI ACTGGGnnnn_n'
Cuts at: 681 1709 2696 2721 2926 4264 5126 5716 5797
Size: 1028 987 25 205 1338 862 590 81
Cuts at: 5797 6132 8276 681
Size: 335 2144 909
Fragments arranged by size:
2144 1338 1028 987 909 862 590 335
205 81 25
BmtI G_CTAG'C
Cuts at: 4322 4322
Size: 8504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 1197 1755 3039 4668 6871 1101
Size: 96 558 1284 1629 2203 2734
Fragments arranged by size:
2734 2203 1629 1284 558 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 176 297 499 655 811 1009 1240 1249
Size: 9 121 202 156 156 198 231 9
Cuts at: 1249 1300 1417 1477 1849 2096 2482 2833 3259
Size: 51 117 60 372 247 386 351 426
Cuts at: 3259 3275 3293 3353 3516 3874 3898 4071 4115
Size: 16 18 60 163 358 24 173 44
Cuts at: 4115 4175 4393 4514 4889 5020 5425 167
Size: 60 218 121 375 131 405 3246
Fragments arranged by size:
3246 426 405 386 375 372 358 351
247 231 218 202 198 173 163 156
156 131 121 121 117 60 60 60
51 44 24 18 16 9 9
Bpu10I CC'TnA_GC
Cuts at: 487 547 1144 2139 2372 3610 4994 5076 5192
Size: 60 597 995 233 1238 1384 82 116
Cuts at: 5192 487
Size: 3799
Fragments arranged by size:
3799 1384 1238 995 597 233 116 82
60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2311 3284 5089 5680 6559 7273 7571 2311
Size: 973 1805 591 879 714 298 3244
Fragments arranged by size:
3244 1805 973 879 714 591 298
BsaI GGTCTCn'nnnn_
Cuts at: 3251 3587 3986 4660 8208 3251
Size: 336 399 674 3548 3547
Fragments arranged by size:
3548 3547 674 399 336
BsaAI yAC'GTr
Cuts at: 699 5857 699
Size: 5158 3346
BsaBI GATnn'nnATC
Cuts at: 6 2914 6018 8055 6
Size: 2908 3104 2037 455
Fragments arranged by size:
3104 2908 2037 455
BsaHI Gr'CG_yC
Cuts at: 844 2443 3565 5935 6183 6885 8381 844
Size: 1599 1122 2370 248 702 1496 967
Fragments arranged by size:
2370 1599 1496 1122 967 702 248
BsaWI w'CCGG_w
Cuts at: 1334 2271 4669 5693 6214 6892 7303 7450 7724
Size: 937 2398 1024 521 678 411 147 274
Cuts at: 7724 1334
Size: 2114
Fragments arranged by size:
2398 2114 1024 937 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 1475 1505 1828 1858 3487 3517 3952
Size: 30 1150 30 323 30 1629 30 435
Cuts at: 3952 3982 5106 5136 295
Size: 30 1124 30 3663
Fragments arranged by size:
3663 1629 1150 1124 435 323 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 295 652 1039 1093 1342 1737 1837 1895
Size: 62 357 387 54 249 395 100 58
Cuts at: 1895 2245 2248 3260 3403 3436 3605 3739 3742
Size: 350 3 1012 143 33 169 134 3
Cuts at: 3742 3745 3748 3751 3760 3871 3943 3946 3961
Size: 3 3 3 9 111 72 3 15
Cuts at: 3961 3970 3973 3976 4158 4163 4782 5114 5493
Size: 9 3 3 182 5 619 332 379
Cuts at: 5493 5496 233
Size: 3 3241
Fragments arranged by size:
3241 1012 619 395 387 379 357 350
332 249 182 169 143 134 111 100
72 62 58 54 33 15 9 9
5 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 361 731 1262 1598 1854 2581 2801 2850 3059
Size: 370 531 336 256 727 220 49 209
Cuts at: 3059 3262 3394 3408 3557 3750 3821 4020 4026
Size: 203 132 14 149 193 71 199 6
Cuts at: 4026 4335 4584 5017 5171 5929 6687 7352 7760
Size: 309 249 433 154 758 758 665 408
Cuts at: 7760 361
Size: 1105
Fragments arranged by size:
1105 758 758 727 665 531 433 408
370 336 309 256 249 220 209 203
199 193 154 149 132 71 49 14
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 958 1408 1492 1644 2902 239
Size: 719 450 84 152 1258 5841
Fragments arranged by size:
5841 1258 719 450 152 84
BsiEI CG_ry'CG
Cuts at: 104 714 3684 6046 6092 7322 104
Size: 610 2970 2362 46 1230 1286
Fragments arranged by size:
2970 2362 1286 1230 610 46
BsiHKAI G_wGCw'C
Cuts at: 313 959 1439 1493 2033 2255 3318 3552 4357
Size: 646 480 54 540 222 1063 234 805
Cuts at: 4357 5518 6296 6486 7346 313
Size: 1161 778 190 860 1471
Fragments arranged by size:
1471 1161 1063 860 805 778 646 540
480 234 222 190 54
BsmI GAATG_Cn'
Cuts at: 1068 1140 1068
Size: 72 8432
BsmBI CGTCTCn'nnnn_
Cuts at: 296 778 296
Size: 482 8022
Bsp1286I G_dGCh'C
Cuts at: 168 313 495 648 820 861 959 1439 1493
Size: 145 182 153 172 41 98 480 54
Cuts at: 1493 1519 1648 2033 2255 2266 2489 2565 2582
Size: 26 129 385 222 11 223 76 17
Cuts at: 2582 2639 2689 2789 2900 3318 3415 3552 3558
Size: 57 50 100 111 418 97 137 6
Cuts at: 3558 4159 4238 4261 4300 4342 4357 4516 4711
Size: 601 79 23 39 42 15 159 195
Cuts at: 4711 4774 5077 5413 5518 6129 6222 6296 6486
Size: 63 303 336 105 611 93 74 190
Cuts at: 6486 6548 7346 168
Size: 62 798 1326
Fragments arranged by size:
1326 798 611 601 480 418 385 336
303 223 222 195 190 182 172 159
153 145 137 129 111 105 100 98
97 93 79 76 74 63 62 57
54 50 42 41 39 26 23 17
15 11 6
BspEI T'CCGG_A
Cuts at: 1334 1334
Size: 8504
BspHI T'CATG_A
Cuts at: 6936 6936
Size: 8504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1110 2483 2700 3066 3771 4020 5554 5768 6070
Size: 1373 217 366 705 249 1534 214 302
Cuts at: 6070 6451 1110
Size: 381 3163
Fragments arranged by size:
3163 1534 1373 705 381 366 302 249
217 214
BsrBI CCG'CTC
Cuts at: 50 795 4611 5141 6796 6850 8334 8499 50
Size: 745 3816 530 1655 54 1484 165 55
Fragments arranged by size:
3816 1655 1484 745 530 165 55 54
BsrDI GCAATG_nn'
Cuts at: 620 4015 6416 620
Size: 3395 2401 2708
Fragments arranged by size:
3395 2708 2401
BsrFI r'CCGG_y
Cuts at: 4331 6502 6683 4331
Size: 2171 181 6152
Fragments arranged by size:
6152 2171 181
BssSI C'ACGA_G
Cuts at: 3358 3670 6775 7483 3358
Size: 312 3105 708 4379
Fragments arranged by size:
4379 3105 708 312
BstAPI GCAn_nnn'nTGC
Cuts at: 936 3072 3322 5881 936
Size: 2136 250 2559 3559
Fragments arranged by size:
3559 2559 2136 250
BstBI TT'CG_AA
Cuts at: 6865 6865
Size: 8504
BstXI CCAn_nnnn'nTGG
Cuts at: 321 633 4246 4410 5403 321
Size: 312 3613 164 993 3422
Fragments arranged by size:
3613 3422 993 312 164
BstYI r'GATC_y
Cuts at: 1 1524 1889 2909 3860 3872 4194 4811 4901
Size: 1523 365 1020 951 12 322 617 90
Cuts at: 4901 5529 5996 6019 6354 6600 6906 6918 7004
Size: 628 467 23 335 246 306 12 86
Cuts at: 7004 8056 1
Size: 1052 449
Fragments arranged by size:
1523 1052 1020 951 628 617 467 449
365 335 322 306 246 90 86 23
12 12
Bsu36I CC'TnA_GG
Cuts at: 670 1796 2228 4735 5420 670
Size: 1126 432 2507 685 3754
Fragments arranged by size:
3754 2507 1126 685 432
BtgI C'CryG_G
Cuts at: 107 130 152 160 320 3029 3114 3678 4359
Size: 23 22 8 160 2709 85 564 681
Cuts at: 4359 5231 107
Size: 872 3380
Fragments arranged by size:
3380 2709 872 681 564 160 85 23
22 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 216 1032 3866 6454 6635 6798 216
Size: 816 2834 2588 181 163 1922
Fragments arranged by size:
2834 2588 1922 816 181 163
BtsI GCAGTG_nn'
Cuts at: 166 222 2208 2536 3045 3410 4206 4629 4745
Size: 56 1986 328 509 365 796 423 116
Cuts at: 4745 5747 166
Size: 1002 2923
Fragments arranged by size:
2923 1986 1002 796 509 423 365 328
116 56
ClaI AT'CG_AT
Cuts at: 2942 2942
Size: 8504
DraI TTT'AAA
Cuts at: 5503 5503
Size: 8504
DraIII CAC_nnn'GTG
Cuts at: 2346 2346
Size: 8504
DrdI GACnn_nn'nnGTC
Cuts at: 6210 7554 6210
Size: 1344 7160
EaeI y'GGCC_r
Cuts at: 2040 2120 3167 3485 3681 4059 4362 4385 6089
Size: 80 1047 318 196 378 303 23 1704
Cuts at: 6089 6263 6654 6681 8026 2040
Size: 174 391 27 1345 2518
Fragments arranged by size:
2518 1704 1345 1047 391 378 318 303
196 174 80 27 23
EagI C'GGCC_G
Cuts at: 3681 6089 3681
Size: 2408 6096
EarI CTCTTCn'nnn_
Cuts at: 547 569 589 2675 3710 3716 3737 3932 3938
Size: 22 20 2086 1035 6 21 195 6
Cuts at: 3938 4711 4871 5481 6527 6737 547
Size: 773 160 610 1046 210 2314
Fragments arranged by size:
2314 2086 1046 1035 773 610 210 195
160 22 21 20 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1492 3142 4716 6712 7454 7600 8314 1492
Size: 1650 1574 1996 742 146 714 1682
Fragments arranged by size:
1996 1682 1650 1574 742 714 146
EcoICRI GAG'CTC
Cuts at: 311 957 1437 1491 2031 2253 5516 311
Size: 646 480 54 540 222 3263 3299
Fragments arranged by size:
3299 3263 646 540 480 222 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 176 297 359 457 499 613 655 811
Size: 9 121 62 98 42 114 42 156
Cuts at: 811 1009 1240 1249 1261 1300 1379 1417 1477
Size: 198 231 9 12 39 79 38 60
Cuts at: 1477 1849 2096 2482 2833 3221 3259 3275 3293
Size: 372 247 386 351 388 38 16 18
Cuts at: 3293 3353 3516 3874 3898 4071 4115 4175 4393
Size: 60 163 358 24 173 44 60 218
Cuts at: 4393 4514 4889 4927 5020 5425 6329 6761 7114
Size: 121 375 38 93 405 904 432 353
Cuts at: 7114 7776 167
Size: 662 895
Fragments arranged by size:
904 895 662 432 405 388 386 375
372 358 353 351 247 231 218 198
173 163 156 121 121 114 98 93
79 62 60 60 60 44 42 42
39 38 38 38 24 18 16 12
9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 402 529 991 1075 1483 5423 402
Size: 127 462 84 408 3940 3483
Fragments arranged by size:
3940 3483 462 408 127 84
EcoO109I rG'GnC_Cy
Cuts at: 141 491 492 644 682 817 857 858 1203
Size: 350 1 152 38 135 40 1 345
Cuts at: 1203 1516 1704 1933 2049 2143 2277 2427 2434
Size: 313 188 229 116 94 134 150 7
Cuts at: 2434 2486 2508 2785 2786 2897 3250 3353 3363
Size: 52 22 277 1 111 353 103 10
Cuts at: 3363 3459 4235 4258 4296 4297 4339 4540 4561
Size: 96 776 23 38 1 42 201 21
Cuts at: 4561 4707 4708 5164 5185 5382 5409 5410 5643
Size: 146 1 456 21 197 27 1 233
Cuts at: 5643 141
Size: 3002
Fragments arranged by size:
3002 776 456 353 350 345 313 277
233 229 201 197 188 152 150 146
135 134 116 111 103 96 94 52
42 40 38 38 27 23 22 21
21 10 7 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5508 5508
Size: 8504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 576 1975 3930 4897 6523 576
Size: 1399 1955 967 1626 2557
Fragments arranged by size:
2557 1955 1626 1399 967
FspI TGC'GCA
Cuts at: 772 3100 6285 772
Size: 2328 3185 2991
Fragments arranged by size:
3185 2991 2328
FspAI rTGC'GCAy
Cuts at: 3100 3100
Size: 8504
HaeII r_GCGC'y
Cuts at: 516 633 847 1659 2446 3229 3568 4372 5938
Size: 117 214 812 787 783 339 804 1566
Cuts at: 5938 6186 7416 8187 516
Size: 248 1230 771 833
Fragments arranged by size:
1566 1230 833 812 804 787 783 771
339 248 214 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 180 212 295 327 1828 1860 1874 1906 2065
Size: 32 83 32 1501 32 14 32 159
Cuts at: 2065 2097 3250 3282 3586 3618 3952 3984 4828
Size: 32 1153 32 304 32 334 32 844
Cuts at: 4828 4860 6346 6376 6378 6408 180
Size: 32 1486 30 2 30 2276
Fragments arranged by size:
2276 1501 1486 1153 844 334 304 159
83 32 32 32 32 32 32 32
32 32 30 30 14 2
HincII GTy'rAC
Cuts at: 2606 5543 2606
Size: 2937 5567
KasI G'GCGC_C
Cuts at: 843 2442 3564 5934 6182 843
Size: 1599 1122 2370 248 3165
Fragments arranged by size:
3165 2370 1599 1122 248
KpnI G_GTAC'C
Cuts at: 5524 5524
Size: 8504
MluI A'CGCG_T
Cuts at: 7937 7937
Size: 8504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1766 2063 2984 5138 5420 7263 7447 1766
Size: 297 921 2154 282 1843 184 2823
Fragments arranged by size:
2823 2154 1843 921 297 282 184
MscI TGG'CCA
Cuts at: 3487 4061 4387 6265 3487
Size: 574 326 1878 5726
Fragments arranged by size:
5726 1878 574 326
MslI CAynn'nnrTG
Cuts at: 2402 2632 3016 5323 6493 6620 2402
Size: 230 384 2307 1170 127 4286
Fragments arranged by size:
4286 2307 1170 384 230 127
MspA1I CmG'CkG
Cuts at: 735 780 945 1041 1266 1278 1392 1858 1965
Size: 45 165 96 225 12 114 466 107
Cuts at: 1965 1972 2334 2589 2820 3063 3069 4391 4460
Size: 7 362 255 231 243 6 1322 69
Cuts at: 4460 5040 5615 5929 6289 7073 7318 735
Size: 580 575 314 360 784 245 1921
Fragments arranged by size:
1921 1322 784 580 575 466 362 360
314 255 245 243 231 225 165 114
107 96 69 45 12 7 6
NaeI GCC'GGC
Cuts at: 4333 6685 4333
Size: 2352 6152
NarI GG'CG_CC
Cuts at: 844 2443 3565 5935 6183 844
Size: 1599 1122 2370 248 3165
Fragments arranged by size:
3165 2370 1599 1122 248
NcoI C'CATG_G
Cuts at: 107 152 160 5231 107
Size: 45 8 5071 3380
Fragments arranged by size:
5071 3380 45 8
NgoMIV G'CCGG_C
Cuts at: 4331 6683 4331
Size: 2352 6152
NheI G'CTAG_C
Cuts at: 4318 4318
Size: 8504
NspI r_CATG'y
Cuts at: 1251 4116 5557 6057 7660 1251
Size: 2865 1441 500 1603 2095
Fragments arranged by size:
2865 2095 1603 1441 500
PasI CC'CwG_GG
Cuts at: 495 1651 2430 2789 2905 3247 3447 3543 3598
Size: 1156 779 359 116 342 200 96 55
Cuts at: 3598 3617 4535 5413 495
Size: 19 918 878 3586
Fragments arranged by size:
3586 1156 918 878 779 359 342 200
116 96 55 19
PciI A'CATG_T
Cuts at: 7656 7656
Size: 8504
PflMI CCAn_nnn'nTGG
Cuts at: 136 1158 2346 3253 3604 5232 136
Size: 1022 1188 907 351 1628 3408
Fragments arranged by size:
3408 1628 1188 1022 907 351
PfoI T'CCnGG_A
Cuts at: 3309 3707 3757 3875 3923 4345 5402 3309
Size: 398 50 118 48 422 1057 6411
Fragments arranged by size:
6411 1057 422 398 118 50 48
PmeI GTTT'AAAC
Cuts at: 5503 5503
Size: 8504
PmlI CAC'GTG
Cuts at: 699 699
Size: 8504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6950 6982 6950
Size: 32 8472
PpuMI rG'GwC_Cy
Cuts at: 141 682 1704 1933 3353 3363 3459 141
Size: 541 1022 229 1420 10 96 5186
Fragments arranged by size:
5186 1420 1022 541 229 96 10
PshAI GACnn'nnGTC
Cuts at: 4040 4040
Size: 8504
PspOMI G'GGCC_C
Cuts at: 491 644 816 857 1515 2485 2785 2896 3554
Size: 153 172 41 658 970 300 111 658
Cuts at: 3554 4234 4257 4296 4338 4707 5409 491
Size: 680 23 39 42 369 702 3586
Fragments arranged by size:
3586 970 702 680 658 658 369 300
172 153 111 42 41 39 23
PstI C_TGCA'G
Cuts at: 260 659 755 1040 1211 1346 2837 3068 3077
Size: 399 96 285 171 135 1491 231 9
Cuts at: 3077 3513 4213 4752 5551 6236 260
Size: 436 700 539 799 685 2528
Fragments arranged by size:
2528 1491 799 700 685 539 436 399
285 231 171 135 96 9
PvuI CG_AT'CG
Cuts at: 104 714 104
Size: 610 7894
PvuII CAG'CTG
Cuts at: 945 1041 1278 1392 1858 1972 2334 2589 3063
Size: 96 237 114 466 114 362 255 474
Cuts at: 3063 3069 4391 5929 6289 945
Size: 6 1322 1538 360 3160
Fragments arranged by size:
3160 1538 1322 474 466 362 360 255
237 114 114 96 6
RsrII CG'GwC_CG
Cuts at: 6699 6699
Size: 8504
SacI G_AGCT'C
Cuts at: 313 959 1439 1493 2033 2255 5518 313
Size: 646 480 54 540 222 3263 3299
Fragments arranged by size:
3299 3263 646 540 480 222 54
SalI G'TCGA_C
Cuts at: 5541 5541
Size: 8504
SanDI GG'GwC_CC
Cuts at: 141 682 1704 1933 3353 141
Size: 541 1022 229 1420 5292
Fragments arranged by size:
5292 1420 1022 541 229
SapI GCTCTTCn'nnn_
Cuts at: 547 4871 6527 6737 547
Size: 4324 1656 210 2314
Fragments arranged by size:
4324 2314 1656 210
SbfI CC_TGCA'GG
Cuts at: 5551 5551
Size: 8504
ScaI AGT'ACT
Cuts at: 8021 8021
Size: 8504
SfcI C'TryA_G
Cuts at: 33 256 655 751 1036 1207 1342 2833 3064
Size: 223 399 96 285 171 135 1491 231
Cuts at: 3064 3073 3509 3900 4209 4523 4748 5547 6232
Size: 9 436 391 309 314 225 799 685
Cuts at: 6232 7200 7391 33
Size: 968 191 1146
Fragments arranged by size:
1491 1146 968 799 685 436 399 391
314 309 285 231 225 223 191 171
135 96 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 3526 4337 4539 2048
Size: 1478 811 202 6013
Fragments arranged by size:
6013 1478 811 202
SfoI GGC'GCC
Cuts at: 845 2444 3566 5936 6184 845
Size: 1599 1122 2370 248 3165
Fragments arranged by size:
3165 2370 1599 1122 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8504
SmaI CCC'GGG
Cuts at: 815 855 863 1426 1514 2566 4657 5526 815
Size: 40 8 563 88 1052 2091 869 3793
Fragments arranged by size:
3793 2091 1052 869 563 88 40 8
SmlI C'TyrA_G
Cuts at: 526 2326 3299 5068 5659 6574 7288 7550 526
Size: 1800 973 1769 591 915 714 262 1480
Fragments arranged by size:
1800 1769 1480 973 915 714 591 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8504
SphI G_CATG'C
Cuts at: 5557 6057 5557
Size: 500 8004
SrfI GCCC'GGGC
Cuts at: 2566 2566
Size: 8504
StuI AGG'CCT
Cuts at: 3520 4243 4507 3520
Size: 723 264 7517
Fragments arranged by size:
7517 723 264
StyI C'CwwG_G
Cuts at: 107 152 160 1406 1520 1557 2450 2781 3291
Size: 45 8 1246 114 37 893 331 510
Cuts at: 3291 3585 4640 5231 5638 5992 107
Size: 294 1055 591 407 354 2619
Fragments arranged by size:
2619 1246 1055 893 591 510 407 354
331 294 114 45 37 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2463 2548 2622 3042 3221 3439 3556 6087 6220
Size: 85 74 420 179 218 117 2531 133
Cuts at: 6220 6679 6888 2463
Size: 459 209 4079
Fragments arranged by size:
4079 2531 459 420 218 209 179 133
117 85 74
TatI w'GTAC_w
Cuts at: 1979 3042 5096 8019 1979
Size: 1063 2054 2923 2464
Fragments arranged by size:
2923 2464 2054 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1475 1507 1744 1776 3487 3519 8021 8053 1475
Size: 32 237 32 1711 32 4502 32 1926
Fragments arranged by size:
4502 1926 1711 237 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 6301 1840
Size: 4461 4043
XbaI T'CTAG_A
Cuts at: 5535 5535
Size: 8504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2457 2734 3391 4951 5280 5999 2457
Size: 277 657 1560 329 719 4962
Fragments arranged by size:
4962 1560 719 657 329 277
XmaI C'CCGG_G
Cuts at: 813 853 861 1424 1512 2564 4655 5524 813
Size: 40 8 563 88 1052 2091 869 3793
Fragments arranged by size:
3793 2091 1052 869 563 88 40 8
XmnI GAAnn'nnTTC
Cuts at: 4880 4880
Size: 8504
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AscI AvrII BaeI BfrBI BsiWI BsrGI
BssHII BstEII BstZ17I CspCI EcoRV FseI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PsiI PspXI
PsrI SacII SexAI SgrAI SnaBI SspI SwaI XhoI
ZraI