Restriction map for
pF1KA0614
(Circular) (Six-base) MAPSORT of: pF1KA0614.seq Check: 3789 from: 1 to: 12113
pF1KA0614 12113 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1173 4568 5298 8079 8722 8899 1173
Size: 3395 730 2781 643 177 4387
Fragments arranged by size:
4387 3395 2781 730 643 177
AatII G_ACGT'C
Cuts at: 3443 8264 3443
Size: 4821 7292
AccI GT'mk_AC
Cuts at: 7747 9151 7747
Size: 1404 10709
Acc65I G'GTAC_C
Cuts at: 9129 9129
Size: 12113
AclI AA'CG_TT
Cuts at: 11953 11953
Size: 12113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 2272 7792 9938 10370 11117 11385 781
Size: 1491 5520 2146 432 747 268 1509
Fragments arranged by size:
5520 2146 1509 1491 747 432 268
AfeI AGC'GCT
Cuts at: 3030 3591 3030
Size: 561 11552
AflII C'TTAA_G
Cuts at: 7227 7227
Size: 12113
AflIII A'CryG_T
Cuts at: 826 4334 5051 9465 10569 11546 826
Size: 3508 717 4414 1104 977 1393
Fragments arranged by size:
4414 3508 1393 1104 977 717
AgeI A'CCGG_T
Cuts at: 10501 10501
Size: 12113
AleI CACnn'nnGTG
Cuts at: 7807 9086 7807
Size: 1279 10834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 629 4489 629
Size: 3860 8253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 2351
Size: 32 12081
AlwNI CAG_nnn'CTG
Cuts at: 382 555 714 1159 1972 2665 2782 5232 5976
Size: 173 159 445 813 693 117 2450 744
Cuts at: 5976 6018 6915 7068 8217 8238 8442 8676 9418
Size: 42 897 153 1149 21 204 234 742
Cuts at: 9418 10985 382
Size: 1567 1510
Fragments arranged by size:
2450 1567 1510 1149 897 813 744 742
693 445 234 204 173 159 153 117
42 21
ApaI G_GGCC'C
Cuts at: 6028 8817 6028
Size: 2789 9324
ApaLI G'TGCA_C
Cuts at: 718 1162 2324 3253 3564 5407 10883 718
Size: 444 1162 929 311 1843 5476 1948
Fragments arranged by size:
5476 1948 1843 1162 929 444 311
ApoI r'AATT_y
Cuts at: 213 481 1647 2387 2758 3771 4015 4195 4412
Size: 268 1166 740 371 1013 244 180 217
Cuts at: 4412 4875 6659 7363 9117 11466 11722 213
Size: 463 1784 704 1754 2349 256 604
Fragments arranged by size:
2349 1784 1754 1166 1013 740 704 604
463 371 268 256 244 217 180
AseI AT'TA_AT
Cuts at: 20 20
Size: 12113
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12113
AvaI C'yCGr_G
Cuts at: 1214 3047 3494 4882 5896 6223 6400 6641 8184
Size: 1833 447 1388 1014 327 177 241 1543
Cuts at: 8184 8737 9133 1214
Size: 553 396 4194
Fragments arranged by size:
4194 1833 1543 1388 1014 553 447 396
327 241 177
BamHI G'GATC_C
Cuts at: 1509 2359 6062 6381 9138 1509
Size: 850 3703 319 2757 4484
Fragments arranged by size:
4484 3703 2757 850 319
BanI G'GyrC_C
Cuts at: 625 1804 2142 3646 4606 4956 5429 5554 5633
Size: 1179 338 1504 960 350 473 125 79
Cuts at: 5633 6097 6852 8056 9129 9543 9791 9826 625
Size: 464 755 1204 1073 414 248 35 2912
Fragments arranged by size:
2912 1504 1204 1179 1073 960 755 473
464 414 350 338 248 125 79 35
BanII G_rGCy'C
Cuts at: 1463 2950 3761 5396 5510 5569 6028 6224 6434
Size: 1487 811 1635 114 59 459 196 210
Cuts at: 6434 6518 6559 6802 8191 8318 8458 8656 8817
Size: 84 41 243 1389 127 140 198 161
Cuts at: 8817 9127 10157 1463
Size: 310 1030 3419
Fragments arranged by size:
3419 1635 1487 1389 1030 811 459 310
243 210 198 196 161 140 127 114
84 59 41
BbeI G_GCGC'C
Cuts at: 1808 5558 6101 9547 9795 1808
Size: 3750 543 3446 248 4126
Fragments arranged by size:
4126 3750 3446 543 248
BbsI GAAGACnn'nnnn_
Cuts at: 1270 1448 1736 2744 2928 3443 3924 4079 5834
Size: 178 288 1008 184 515 481 155 1755
Cuts at: 5834 6126 6176 6235 8305 9098 1270
Size: 292 50 59 2070 793 4285
Fragments arranged by size:
4285 2070 1755 1008 793 515 481 292
288 184 178 155 59 50
BbvCI CC'TCA_GC
Cuts at: 2863 5697 2863
Size: 2834 9279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 302 336 302
Size: 34 12079
BciVI GTATCCnnnnn_n'
Cuts at: 4334 10011 10772 12113 4334
Size: 5677 761 1341 4334
Fragments arranged by size:
5677 4334 1341 761
BclI T'GATC_A
Cuts at: 9020 9633 9020
Size: 613 11500
BfrBI ATG'CAT
Cuts at: 7457 7457
Size: 12113
BglI GCCn_nnn'nGGC
Cuts at: 1084 6013 8641 1084
Size: 4929 2628 4556
Fragments arranged by size:
4929 4556 2628
BglII A'GATC_T
Cuts at: 4790 6146 9628 11665 4790
Size: 1356 3482 2037 5238
Fragments arranged by size:
5238 3482 2037 1356
BlpI GC'TnA_GC
Cuts at: 3713 3760 5875 6916 8145 8686 9225 3713
Size: 47 2115 1041 1229 541 539 6601
Fragments arranged by size:
6601 2115 1229 1041 541 539 47
Bme1580I G_kGCm'C
Cuts at: 722 1166 1917 2328 3055 3223 3257 3568 4968
Size: 444 751 411 727 168 34 311 1400
Cuts at: 4968 5411 6028 6857 6900 7646 8140 8817 8933
Size: 443 617 829 43 746 494 677 116
Cuts at: 8933 9738 9831 10887 722
Size: 805 93 1056 1948
Fragments arranged by size:
1948 1400 1056 829 805 751 746 727
677 617 494 444 443 411 311 168
116 93 43 34
BmgBI CAC'GTC
Cuts at: 3303 3324 4872 6272 7512 3303
Size: 21 1548 1400 1240 7904
Fragments arranged by size:
7904 1548 1400 1240 21
BmrI ACTGGGnnnn_n'
Cuts at: 312 3114 5078 5152 5550 9027 9325 9406 9741
Size: 2802 1964 74 398 3477 298 81 335
Cuts at: 9741 11885 312
Size: 2144 540
Fragments arranged by size:
3477 2802 2144 1964 540 398 335 298
81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1059 2055 4741 6555 8448 10480 1059
Size: 996 2686 1814 1893 2032 2692
Fragments arranged by size:
2692 2686 2032 1893 1814 996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1246 1387 1491 1694 1738 2843 3252 4130
Size: 217 141 104 203 44 1105 409 878
Cuts at: 4130 4573 4602 5183 5215 5437 5446 7066 7425
Size: 443 29 581 32 222 9 1620 359
Cuts at: 7425 7522 7720 8428 8716 8839 9079 1029
Size: 97 198 708 288 123 240 4063
Fragments arranged by size:
4063 1620 1105 878 708 581 443 409
359 288 240 222 217 203 198 141
123 104 97 44 32 29 9
Bpu10I CC'TnA_GC
Cuts at: 1321 2863 5697 8327 1321
Size: 1542 2834 2630 5107
Fragments arranged by size:
5107 2834 2630 1542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1041 1278 6776 7470 8731 9289 10168 10660 10958
Size: 237 5498 694 1261 558 879 492 298
Cuts at: 10958 1041
Size: 2196
Fragments arranged by size:
5498 2196 1261 879 694 558 492 298
237
BsaI GGTCTCn'nnnn_
Cuts at: 862 3886 4043 4129 5550 7696 8392 8998 11817
Size: 3024 157 86 1421 2146 696 606 2819
Cuts at: 11817 862
Size: 1158
Fragments arranged by size:
3024 2819 2146 1421 1158 696 606 157
86
BsaAI yAC'GTr
Cuts at: 513 5609 9466 513
Size: 5096 3857 3160
Fragments arranged by size:
5096 3857 3160
BsaBI GATnn'nnATC
Cuts at: 6 1667 1714 6151 9627 11664 6
Size: 1661 47 4437 3476 2037 455
Fragments arranged by size:
4437 3476 2037 1661 455 47
BsaHI Gr'CG_yC
Cuts at: 504 1805 3440 5555 5993 6098 8261 9544 9792
Size: 1301 1635 2115 438 105 2163 1283 248
Cuts at: 9792 10494 11990 504
Size: 702 1496 627
Fragments arranged by size:
2163 2115 1635 1496 1301 1283 702 627
438 248 105
BsaWI w'CCGG_w
Cuts at: 3148 8588 9302 9823 10501 10775 10922 11333 3148
Size: 5440 714 521 678 274 147 411 3928
Fragments arranged by size:
5440 3928 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2836 2866 3348 3378 3493 3523 4128 4158 4580
Size: 30 482 30 115 30 605 30 422
Cuts at: 4580 4610 5713 5743 6541 6571 8401 8431 8827
Size: 30 1103 30 798 30 1830 30 396
Cuts at: 8827 8857 2836
Size: 30 6092
Fragments arranged by size:
6092 1830 1103 798 605 482 422 396
115 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1339 3356 4573 4583 4777 4932 5439 5542 5721
Size: 2017 1217 10 194 155 507 103 179
Cuts at: 5721 5724 5727 5767 5811 6991 8059 8221 8527
Size: 3 3 40 44 1180 1068 162 306
Cuts at: 8527 8851 9030 1339
Size: 324 179 4422
Fragments arranged by size:
4422 2017 1217 1180 1068 507 324 306
194 179 179 162 155 103 44 40
10 3 3
BseYI C'CCAG_C
Cuts at: 1246 1513 1521 2726 3025 3031 3338 4350 5058
Size: 267 8 1205 299 6 307 1012 708
Cuts at: 5058 5770 5976 8140 8238 8435 9538 10296 10873
Size: 712 206 2164 98 197 1103 758 577
Cuts at: 10873 11369 1246
Size: 496 1990
Fragments arranged by size:
2164 1990 1205 1103 1012 758 712 708
577 496 307 299 267 206 197 98
8 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 1015 4294 4735 6495 7543 7864 8203 8547
Size: 359 3279 441 1760 1048 321 339 344
Cuts at: 8547 656
Size: 4222
Fragments arranged by size:
4222 3279 1760 1048 441 359 344 339
321
BsiEI CG_ry'CG
Cuts at: 104 3246 3517 5585 5705 7030 8636 9655 9701
Size: 3142 271 2068 120 1325 1606 1019 46
Cuts at: 9701 10909 104
Size: 1208 1308
Fragments arranged by size:
3142 2068 1606 1325 1308 1208 1019 271
120 46
BsiHKAI G_wGCw'C
Cuts at: 722 900 1166 1463 2205 2328 2889 3257 3568
Size: 178 266 297 742 123 561 368 311
Cuts at: 3568 3761 4559 4976 5396 5411 5510 5876 7159
Size: 193 798 417 420 15 99 366 1283
Cuts at: 7159 7205 9127 9905 10095 10887 722
Size: 46 1922 778 190 792 1948
Fragments arranged by size:
1948 1922 1283 798 792 778 742 561
420 417 368 366 311 297 266 193
190 178 123 99 46 15
BsiWI C'GTAC_G
Cuts at: 747 747
Size: 12113
BsmI GAATG_Cn'
Cuts at: 2243 7839 2243
Size: 5596 6517
BsmBI CGTCTCn'nnnn_
Cuts at: 1382 3447 5524 6526 7979 8776 1382
Size: 2065 2077 1002 1453 797 4719
Fragments arranged by size:
4719 2077 2065 1453 1002 797
Bsp1286I G_dGCh'C
Cuts at: 722 900 1166 1463 1917 2205 2328 2889 2950
Size: 178 266 297 454 288 123 561 61
Cuts at: 2950 3055 3223 3257 3568 3761 4559 4968 4976
Size: 105 168 34 311 193 798 409 8
Cuts at: 4976 5396 5411 5510 5569 5876 6028 6224 6434
Size: 420 15 99 59 307 152 196 210
Cuts at: 6434 6518 6559 6802 6857 6900 7159 7205 7646
Size: 84 41 243 55 43 259 46 441
Cuts at: 7646 8140 8191 8318 8458 8656 8817 8933 9127
Size: 494 51 127 140 198 161 116 194
Cuts at: 9127 9738 9831 9905 10095 10157 10887 722
Size: 611 93 74 190 62 730 1948
Fragments arranged by size:
1948 798 730 611 561 494 454 441
420 409 311 307 297 288 266 259
243 210 198 196 194 193 190 178
168 161 152 140 127 123 116 105
99 93 84 74 62 61 59 55
51 46 43 41 34 15 8
BspEI T'CCGG_A
Cuts at: 8588 11333 8588
Size: 2745 9368
BspHI T'CATG_A
Cuts at: 9017 11289 9017
Size: 2272 9841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 837 1173 4266 4568 5298 5588 8079 8345 8474
Size: 336 3093 302 730 290 2491 266 129
Cuts at: 8474 8722 8899 8962 9016 9163 9377 9679 10060
Size: 248 177 63 54 147 214 302 381
Cuts at: 10060 837
Size: 2890
Fragments arranged by size:
3093 2890 2491 730 381 336 302 302
290 266 248 214 177 147 129 63
54
BsrBI CCG'CTC
Cuts at: 50 3712 3942 4142 10405 10459 11943 12108 50
Size: 3662 230 200 6263 54 1484 165 55
Fragments arranged by size:
6263 3662 1484 230 200 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1759 2539 3555 3809 3936 4650 10025 1759
Size: 780 1016 254 127 714 5375 3847
Fragments arranged by size:
5375 3847 1016 780 714 254 127
BsrFI r'CCGG_y
Cuts at: 5584 6394 6481 8537 8644 10111 10292 10501 5584
Size: 810 87 2056 107 1467 181 209 7196
Fragments arranged by size:
7196 2056 1467 810 209 181 107 87
BsrGI T'GTAC_A
Cuts at: 2210 4949 6563 2210
Size: 2739 1614 7760
Fragments arranged by size:
7760 2739 1614
BssSI C'ACGA_G
Cuts at: 330 10384 10742 330
Size: 10054 358 1701
Fragments arranged by size:
10054 1701 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2323 6113 8217 8337 9490 2323
Size: 3790 2104 120 1153 4946
Fragments arranged by size:
4946 3790 2104 1153 120
BstBI TT'CG_AA
Cuts at: 4116 10474 4116
Size: 6358 5755
BstEII G'GTnAC_C
Cuts at: 5587 5691 7809 8005 5587
Size: 104 2118 196 9695
Fragments arranged by size:
9695 2118 196 104
BstXI CCAn_nnnn'nTGG
Cuts at: 1246 4383 5349 5490 6075 7956 1246
Size: 3137 966 141 585 1881 5403
Fragments arranged by size:
5403 3137 1881 966 585 141
BstYI r'GATC_y
Cuts at: 1 1509 1668 2359 4790 6062 6146 6381 6637
Size: 1508 159 691 2431 1272 84 235 256
Cuts at: 6637 7064 9138 9605 9628 9963 10209 11221 11307
Size: 427 2074 467 23 335 246 1012 86
Cuts at: 11307 11319 11665 1
Size: 12 346 449
Fragments arranged by size:
2431 2074 1508 1272 1012 691 467 449
427 346 335 256 246 235 159 86
84 23 12
BstZ17I GTA'TAC
Cuts at: 7748 7748
Size: 12113
Bsu36I CC'TnA_GG
Cuts at: 2606 3854 7069 7328 2606
Size: 1248 3215 259 7391
Fragments arranged by size:
7391 3215 1248 259
BtgI C'CryG_G
Cuts at: 1082 3617 3701 4679 4960 5044 5342 5852 6123
Size: 2535 84 978 281 84 298 510 271
Cuts at: 6123 7601 8052 8447 1082
Size: 1478 451 395 4748
Fragments arranged by size:
4748 2535 1478 978 510 451 395 298
281 271 84 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 294 3842 5982 7743 10063 10244 10407 294
Size: 3548 2140 1761 2320 181 163 2000
Fragments arranged by size:
3548 2320 2140 2000 1761 181 163
BtsI GCAGTG_nn'
Cuts at: 990 2271 3076 4176 4302 8918 9356 990
Size: 1281 805 1100 126 4616 438 3747
Fragments arranged by size:
4616 3747 1281 1100 805 438 126
ClaI AT'CG_AT
Cuts at: 7460 7460
Size: 12113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2888 2923 2888
Size: 35 12078
DraI TTT'AAA
Cuts at: 9112 9112
Size: 12113
DraIII CAC_nnn'GTG
Cuts at: 1226 1910 2323 4998 6441 7143 1226
Size: 684 413 2675 1443 702 6196
Fragments arranged by size:
6196 2675 1443 702 684 413
DrdI GACnn_nn'nnGTC
Cuts at: 9819 10677 9819
Size: 858 11255
EaeI y'GGCC_r
Cuts at: 557 1076 3243 3457 3514 3704 4448 4733 4989
Size: 519 2167 214 57 190 744 285 256
Cuts at: 4989 5010 5480 5582 5645 7027 7994 8444 8633
Size: 21 470 102 63 1382 967 450 189
Cuts at: 8633 9698 9872 10263 10290 11635 557
Size: 1065 174 391 27 1345 1035
Fragments arranged by size:
2167 1382 1345 1065 1035 967 744 519
470 450 391 285 256 214 190 189
174 102 63 57 27 21
EagI C'GGCC_G
Cuts at: 3243 3514 5582 7027 8633 9698 3243
Size: 271 2068 1445 1606 1065 5658
Fragments arranged by size:
5658 2068 1606 1445 1065 271
EarI CTCTTCn'nnn_
Cuts at: 262 959 1673 1871 2552 3280 3575 4022 4525
Size: 697 714 198 681 728 295 447 503
Cuts at: 4525 4829 6740 7015 10136 10346 262
Size: 304 1911 275 3121 210 2029
Fragments arranged by size:
3121 2029 1911 728 714 697 681 503
447 304 295 275 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2954 4665 5935 6300 8062 10321 10631 10777 11923
Size: 1711 1270 365 1762 2259 310 146 1146
Cuts at: 11923 2954
Size: 3144
Fragments arranged by size:
3144 2259 1762 1711 1270 1146 365 310
146
EcoICRI GAG'CTC
Cuts at: 1461 3759 5394 5508 9125 1461
Size: 2298 1635 114 3617 4449
Fragments arranged by size:
4449 3617 2298 1635 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1029 1246 1387 1491 1694 1738 2272 2843
Size: 248 217 141 104 203 44 534 571
Cuts at: 2843 3252 4130 4573 4602 5183 5215 5437 5446
Size: 409 878 443 29 581 32 222 9
Cuts at: 5446 7066 7425 7522 7720 7792 8428 8716 8839
Size: 1620 359 97 198 72 636 288 123
Cuts at: 8839 9079 9938 10370 11117 11385 781
Size: 240 859 432 747 268 1509
Fragments arranged by size:
1620 1509 878 859 747 636 581 571
534 443 432 409 359 288 268 248
240 222 217 203 198 141 123 104
97 72 44 32 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2645 4753 5026 5305 6079 7771 8086 8481 2645
Size: 2108 273 279 774 1692 315 395 6277
Fragments arranged by size:
6277 2108 1692 774 395 315 279 273
EcoO109I rG'GnC_Cy
Cuts at: 347 805 3090 3603 3971 4346 5964 6015 6025
Size: 458 2285 513 368 375 1618 51 10
Cuts at: 6025 7290 8412 8813 8814 8911 9252 347
Size: 1265 1122 401 1 97 341 3208
Fragments arranged by size:
3208 2285 1618 1265 1122 513 458 401
375 368 341 97 51 10 1
EcoRI G'AATT_C
Cuts at: 213 1647 2758 9117 213
Size: 1434 1111 6359 3209
Fragments arranged by size:
6359 3209 1434 1111
EcoRV GAT'ATC
Cuts at: 398 7392 8352 398
Size: 6994 960 4159
Fragments arranged by size:
6994 4159 960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1668 3990 10132 1668
Size: 2322 6142 3649
Fragments arranged by size:
6142 3649 2322
FseI GG_CCGG'CC
Cuts at: 8648 8648
Size: 12113
FspI TGC'GCA
Cuts at: 918 5401 7452 8710 9894 918
Size: 4483 2051 1258 1184 3137
Fragments arranged by size:
4483 3137 2051 1258 1184
FspAI rTGC'GCAy
Cuts at: 7452 7452
Size: 12113
HaeII r_GCGC'y
Cuts at: 1423 1808 3032 3420 3593 5558 5838 6101 6308
Size: 385 1224 388 173 1965 280 263 207
Cuts at: 6308 9547 9795 10817 11796 1423
Size: 3239 248 1022 979 1740
Fragments arranged by size:
3239 1965 1740 1224 1022 979 388 385
280 263 248 207 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1723 1755 3348 3380 3931 3963 5713 5745 6086
Size: 32 1593 32 551 32 1750 32 341
Cuts at: 6086 6118 6541 6573 6622 6654 7895 7927 9955
Size: 32 423 32 49 32 1241 32 2028
Cuts at: 9955 9985 9987 10017 1723
Size: 30 2 30 3819
Fragments arranged by size:
3819 2028 1750 1593 1241 551 423 341
49 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4863 6740 9152 4863
Size: 1877 2412 7824
Fragments arranged by size:
7824 2412 1877
HindIII A'AGCT_T
Cuts at: 9025 9025
Size: 12113
KasI G'GCGC_C
Cuts at: 1804 5554 6097 9543 9791 1804
Size: 3750 543 3446 248 4126
Fragments arranged by size:
4126 3750 3446 543 248
KpnI G_GTAC'C
Cuts at: 9133 9133
Size: 12113
MfeI C'AATT_G
Cuts at: 2221 4103 2221
Size: 1882 10231
MluI A'CGCG_T
Cuts at: 5051 11546 5051
Size: 6495 5618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1624 1815 4158 4319 5314 5483 6407 6527 7491
Size: 191 2343 161 995 169 924 120 964
Cuts at: 7491 10784 10968 1624
Size: 3293 184 2769
Fragments arranged by size:
3293 2769 2343 995 964 924 191 184
169 161 120
MscI TGG'CCA
Cuts at: 559 1078 3459 3706 4450 4735 5012 5482 7996
Size: 519 2381 247 744 285 277 470 2514
Cuts at: 7996 8446 9874 559
Size: 450 1428 2798
Fragments arranged by size:
2798 2514 2381 1428 744 519 470 450
285 277 247
MslI CAynn'nnrTG
Cuts at: 3260 3548 4870 5347 5488 7580 7807 8548 8998
Size: 288 1322 477 141 2092 227 741 450
Cuts at: 8998 9016 9086 10102 10229 3260
Size: 18 70 1016 127 5144
Fragments arranged by size:
5144 2092 1322 1016 741 477 450 288
227 141 127 70 18
MspA1I CmG'CkG
Cuts at: 609 742 1118 1407 2782 5016 5046 5092 5229
Size: 133 376 289 1375 2234 30 46 137
Cuts at: 5229 5437 5466 6159 6387 7603 7855 7956 8063
Size: 208 29 693 228 1216 252 101 107
Cuts at: 8063 8149 8214 8235 8268 8673 9224 9538 9898
Size: 86 65 21 33 405 551 314 360
Cuts at: 9898 10911 11156 609
Size: 1013 245 1566
Fragments arranged by size:
2234 1566 1375 1216 1013 693 551 405
376 360 314 289 252 245 228 208
137 133 107 101 86 65 46 33
30 29 21
NaeI GCC'GGC
Cuts at: 6396 8539 8646 10294 6396
Size: 2143 107 1648 8215
Fragments arranged by size:
8215 2143 1648 107
NarI GG'CG_CC
Cuts at: 1805 5555 6098 9544 9792 1805
Size: 3750 543 3446 248 4126
Fragments arranged by size:
4126 3750 3446 543 248
NcoI C'CATG_G
Cuts at: 1082 3617 8052 1082
Size: 2535 4435 5143
Fragments arranged by size:
5143 4435 2535
NdeI CA'TA_TG
Cuts at: 8016 8016
Size: 12113
NgoMIV G'CCGG_C
Cuts at: 6394 8537 8644 10292 6394
Size: 2143 107 1648 8215
Fragments arranged by size:
8215 2143 1648 107
NruI TCG'CGA
Cuts at: 2955 2955
Size: 12113
NsiI A_TGCA'T
Cuts at: 7459 7459
Size: 12113
NspI r_CATG'y
Cuts at: 304 438 726 830 2089 2714 2985 3456 4338
Size: 134 288 104 1259 625 271 471 882
Cuts at: 4338 5907 7457 7474 9015 9166 9666 10573 304
Size: 1569 1550 17 1541 151 500 907 1844
Fragments arranged by size:
1844 1569 1550 1541 1259 907 882 625
500 471 288 271 151 134 104 17
PasI CC'CwG_GG
Cuts at: 6802 6802
Size: 12113
PciI A'CATG_T
Cuts at: 826 4334 10569 826
Size: 3508 6235 2370
Fragments arranged by size:
6235 3508 2370
PflMI CCAn_nnn'nTGG
Cuts at: 1088 1910 5161 5385 6018 8442 1088
Size: 822 3251 224 633 2424 4759
Fragments arranged by size:
4759 3251 2424 822 633 224
PfoI T'CCnGG_A
Cuts at: 851 1262 1575 1671 3369 4538 5160 5236 6058
Size: 411 313 96 1698 1169 622 76 822
Cuts at: 6058 851
Size: 6906
Fragments arranged by size:
6906 1698 1169 822 622 411 313 96
76
PmeI GTTT'AAAC
Cuts at: 9112 9112
Size: 12113
PmlI CAC'GTG
Cuts at: 513 513
Size: 12113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5530 5562 11252 11284 5530
Size: 32 5690 32 6359
Fragments arranged by size:
6359 5690 32 32
PpuMI rG'GwC_Cy
Cuts at: 347 805 5964 7290 8911 347
Size: 458 5159 1326 1621 3549
Fragments arranged by size:
5159 3549 1621 1326 458
PshAI GACnn'nnGTC
Cuts at: 790 5296 790
Size: 4506 7607
PsiI TTA'TAA
Cuts at: 3749 3749
Size: 12113
PspOMI G'GGCC_C
Cuts at: 6024 8813 6024
Size: 2789 9324
PspXI vC'TCGA_Gb
Cuts at: 6400 8737 6400
Size: 2337 9776
PstI C_TGCA'G
Cuts at: 551 1887 2109 4808 5309 6164 6340 7682 8108
Size: 1336 222 2699 501 855 176 1342 426
Cuts at: 8108 8342 8485 8615 8672 9160 9845 551
Size: 234 143 130 57 488 685 2819
Fragments arranged by size:
2819 2699 1342 1336 855 685 501 488
426 234 222 176 143 130 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12113
PvuII CAG'CTG
Cuts at: 609 742 1407 2782 5016 5229 5466 7956 8149
Size: 133 665 1375 2234 213 237 2490 193
Cuts at: 8149 8214 8235 8673 9538 9898 609
Size: 65 21 438 865 360 2824
Fragments arranged by size:
2824 2490 2234 1375 865 665 438 360
237 213 193 133 65 21
RsrII CG'GwC_CG
Cuts at: 10308 10308
Size: 12113
SacI G_AGCT'C
Cuts at: 1463 3761 5396 5510 9127 1463
Size: 2298 1635 114 3617 4449
Fragments arranged by size:
4449 3617 2298 1635 114
SacII CC_GC'GG
Cuts at: 5047 7604 5047
Size: 2557 9556
SalI G'TCGA_C
Cuts at: 9150 9150
Size: 12113
SanDI GG'GwC_CC
Cuts at: 347 8911 347
Size: 8564 3549
SapI GCTCTTCn'nnn_
Cuts at: 3280 10136 10346 3280
Size: 6856 210 5047
Fragments arranged by size:
6856 5047 210
SbfI CC_TGCA'GG
Cuts at: 5309 8342 9160 5309
Size: 3033 818 8262
Fragments arranged by size:
8262 3033 818
ScaI AGT'ACT
Cuts at: 9037 11630 9037
Size: 2593 9520
SfcI C'TryA_G
Cuts at: 33 547 588 1883 1949 2105 2441 2466 3176
Size: 514 41 1295 66 156 336 25 710
Cuts at: 3176 4804 5305 6160 6336 6764 7678 8104 8338
Size: 1628 501 855 176 428 914 426 234
Cuts at: 8338 8481 8611 8668 9156 9841 10834 11025 33
Size: 143 130 57 488 685 993 191 1121
Fragments arranged by size:
1628 1295 1121 993 914 855 710 685
514 501 488 428 426 336 234 191
176 156 143 130 66 57 41 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 1084 8641 1084
Size: 7557 4556
SfoI GGC'GCC
Cuts at: 1806 5556 6099 9545 9793 1806
Size: 3750 543 3446 248 4126
Fragments arranged by size:
4126 3750 3446 543 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12113
SmaI CCC'GGG
Cuts at: 3049 4884 9135 3049
Size: 1835 4251 6027
Fragments arranged by size:
6027 4251 1835
SmlI C'TyrA_G
Cuts at: 1020 1293 6400 6791 7227 7485 8737 8746 9268
Size: 273 5107 391 436 258 1252 9 522
Cuts at: 9268 10183 10675 10937 1020
Size: 915 492 262 2196
Fragments arranged by size:
5107 2196 1252 915 522 492 436 391
273 262 258 9
SnaBI TAC'GTA
Cuts at: 5609 5609
Size: 12113
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12113
SphI G_CATG'C
Cuts at: 2714 7457 9166 9666 2714
Size: 4743 1709 500 5161
Fragments arranged by size:
5161 4743 1709 500
StuI AGG'CCT
Cuts at: 1925 3041 5818 6112 6328 7483 1925
Size: 1116 2777 294 216 1155 6555
Fragments arranged by size:
6555 2777 1155 1116 294 216
StyI C'CwwG_G
Cuts at: 1082 1847 3383 3617 3896 7187 7880 8052 8318
Size: 765 1536 234 279 3291 693 172 266
Cuts at: 8318 9247 9601 1082
Size: 929 354 3594
Fragments arranged by size:
3594 3291 1536 929 765 693 354 279
266 234 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 254 1048 2472 2933 6085 7075 7391 7755 8416
Size: 794 1424 461 3152 990 316 364 661
Cuts at: 8416 8845 9696 9829 10288 10497 254
Size: 429 851 133 459 209 1870
Fragments arranged by size:
3152 1870 1424 990 851 794 661 461
459 429 364 316 209 133
TatI w'GTAC_w
Cuts at: 2210 2577 3395 4457 4949 6563 7112 7526 9035
Size: 367 818 1062 492 1614 549 414 1509
Cuts at: 9035 11628 2210
Size: 2593 2695
Fragments arranged by size:
2695 2593 1614 1509 1062 818 549 492
414 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 997 1029 2622 2654 2836 2868 4580 4612 5893
Size: 32 1593 32 182 32 1712 32 1281
Cuts at: 5893 5925 6224 6256 6427 6459 8401 8433 8827
Size: 32 299 32 171 32 1942 32 394
Cuts at: 8827 8859 11630 11662 997
Size: 32 2771 32 1448
Fragments arranged by size:
2771 1942 1712 1593 1448 1281 394 299
182 171 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1035 5128 9910 1035
Size: 4093 4782 3238
Fragments arranged by size:
4782 4093 3238
XbaI T'CTAG_A
Cuts at: 2197 2512 9144 2197
Size: 315 6632 5166
Fragments arranged by size:
6632 5166 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 859 4380 4863 8766 9608 859
Size: 3521 483 3903 842 3364
Fragments arranged by size:
3903 3521 3364 842 483
XhoI C'TCGA_G
Cuts at: 6400 8737 6400
Size: 2337 9776
XmaI C'CCGG_G
Cuts at: 3047 4882 9133 3047
Size: 1835 4251 6027
Fragments arranged by size:
6027 4251 1835
XmnI GAAnn'nnTTC
Cuts at: 2243 2475 6749 7355 2243
Size: 232 4274 606 7001
Fragments arranged by size:
7001 4274 606 232
ZraI GAC'GTC
Cuts at: 3441 8262 3441
Size: 4821 7292
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BmtI BssHII HpaI NheI
NotI PacI PsrI SexAI SgrAI SrfI SspI SwaI