Restriction map for
pF1KA0619
(Circular) (Six-base) MAPSORT of: pF1KA0619.seq Check: 6434 from: 1 to: 7277
pF1KA0619 7277 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 360 1456 4245 4315 360
Size: 1096 2789 70 3322
Fragments arranged by size:
3322 2789 1096 70
Acc65I G'GTAC_C
Cuts at: 4293 4293
Size: 7277
AclI AA'CG_TT
Cuts at: 7117 7117
Size: 7277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 1076 1615 1960 2107 2557 2924 3416 5102
Size: 360 539 345 147 450 367 492 1686
Cuts at: 5102 5534 6281 6549 716
Size: 432 747 268 1444
Fragments arranged by size:
1686 1444 747 539 492 450 432 367
360 345 268 147
AfeI AGC'GCT
Cuts at: 2515 2515
Size: 7277
AflIII A'CryG_T
Cuts at: 811 3956 4629 5733 6710 811
Size: 3145 673 1104 977 1378
Fragments arranged by size:
3145 1378 1104 977 673
AgeI A'CCGG_T
Cuts at: 5665 5665
Size: 7277
AleI CACnn'nnGTG
Cuts at: 3271 3271
Size: 7277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1501 1658 1501
Size: 157 7120
AlwNI CAG_nnn'CTG
Cuts at: 508 1216 1831 1890 2878 3798 4582 6149 508
Size: 708 615 59 988 920 784 1567 1636
Fragments arranged by size:
1636 1567 988 920 784 708 615 59
ApaLI G'TGCA_C
Cuts at: 6047 6047
Size: 7277
ApoI r'AATT_y
Cuts at: 258 682 1038 1067 1362 1689 1963 3607 3776
Size: 424 356 29 295 327 274 1644 169
Cuts at: 3776 3835 4101 4281 6630 6886 258
Size: 59 266 180 2349 256 649
Fragments arranged by size:
2349 1644 649 424 356 327 295 274
266 256 180 169 59 29
AseI AT'TA_AT
Cuts at: 20 2030 20
Size: 2010 5267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7277
AvaI C'yCGr_G
Cuts at: 148 4297 148
Size: 4149 3128
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 842 875 842
Size: 33 7244
BamHI G'GATC_C
Cuts at: 4302 4302
Size: 7277
BanI G'GyrC_C
Cuts at: 142 4293 4707 4955 4990 142
Size: 4151 414 248 35 2429
Fragments arranged by size:
4151 2429 414 248 35
BanII G_rGCy'C
Cuts at: 2441 2693 4291 5321 2441
Size: 252 1598 1030 4397
Fragments arranged by size:
4397 1598 1030 252
BbeI G_GCGC'C
Cuts at: 146 4711 4959 146
Size: 4565 248 2464
Fragments arranged by size:
4565 2464 248
BbsI GAAGACnn'nnnn_
Cuts at: 383 383
Size: 7277
BbvCI CC'TCA_GC
Cuts at: 3533 3533
Size: 7277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 1239 1273 2872 2906 415
Size: 34 790 34 1599 34 4786
Fragments arranged by size:
4786 1599 790 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 972 5175 5936 7277 972
Size: 4203 761 1341 972
Fragments arranged by size:
4203 1341 972 761
BclI T'GATC_A
Cuts at: 1176 1665 3350 4797 1176
Size: 489 1685 1447 3656
Fragments arranged by size:
3656 1685 1447 489
BglI GCCn_nnn'nGGC
Cuts at: 2242 3951 2242
Size: 1709 5568
BglII A'GATC_T
Cuts at: 1908 2267 4792 6829 1908
Size: 359 2525 2037 2356
Fragments arranged by size:
2525 2356 2037 359
BlpI GC'TnA_GC
Cuts at: 4389 4389
Size: 7277
Bme1580I G_kGCm'C
Cuts at: 4902 4995 6051 4902
Size: 93 1056 6128
Fragments arranged by size:
6128 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 4489 4570 4905 7049 4489
Size: 81 335 2144 4717
Fragments arranged by size:
4717 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5644 5644
Size: 7277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1771 1954 2128 2677 2872 2971 4154 217
Size: 1554 183 174 549 195 99 1183 3340
Fragments arranged by size:
3340 1554 1183 549 195 183 174 99
Bpu10I CC'TnA_GC
Cuts at: 3533 3533
Size: 7277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3040 3188 4453 5332 5824 6122 3040
Size: 148 1265 879 492 298 4195
Fragments arranged by size:
4195 1265 879 492 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 145 206 626 6981 145
Size: 61 420 6355 441
Fragments arranged by size:
6355 441 420 61
BsaAI yAC'GTr
Cuts at: 812 4630 812
Size: 3818 3459
BsaBI GATnn'nnATC
Cuts at: 6 596 4791 6828 6
Size: 590 4195 2037 455
Fragments arranged by size:
4195 2037 590 455
BsaHI Gr'CG_yC
Cuts at: 143 4708 4956 5658 7154 143
Size: 4565 248 702 1496 266
Fragments arranged by size:
4565 1496 702 266 248
BsaWI w'CCGG_w
Cuts at: 867 4466 4987 5665 5939 6086 6497 867
Size: 3599 521 678 274 147 411 1647
Fragments arranged by size:
3599 1647 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 623 653 1197 1227 205
Size: 30 388 30 544 30 6255
Fragments arranged by size:
6255 544 388 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1579 3959 4048 1579
Size: 2380 89 4808
Fragments arranged by size:
4808 2380 89
BseYI C'CCAG_C
Cuts at: 3341 4164 4702 5460 6037 6533 3341
Size: 823 538 758 577 496 4085
Fragments arranged by size:
4085 823 758 577 538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 7277
BsiEI CG_ry'CG
Cuts at: 104 4819 4865 6073 104
Size: 4715 46 1208 1308
Fragments arranged by size:
4715 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2441 2693 4291 5069 5259 6051 2441
Size: 252 1598 778 190 792 3667
Fragments arranged by size:
3667 1598 792 778 252 190
Bsp1286I G_dGCh'C
Cuts at: 2441 2693 4291 4902 4995 5069 5259 5321 6051
Size: 252 1598 611 93 74 190 62 730
Cuts at: 6051 2441
Size: 3667
Fragments arranged by size:
3667 1598 730 611 252 190 93 74
62
BspEI T'CCGG_A
Cuts at: 6497 6497
Size: 7277
BspHI T'CATG_A
Cuts at: 1668 2939 3230 6453 1668
Size: 1271 291 3223 2492
Fragments arranged by size:
3223 2492 1271 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4327 4541 4843 5224 4327
Size: 214 302 381 6380
Fragments arranged by size:
6380 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5569 5623 7107 7272 50
Size: 5519 54 1484 165 55
Fragments arranged by size:
5519 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1485 3580 5189 1485
Size: 2095 1609 3573
Fragments arranged by size:
3573 2095 1609
BsrFI r'CCGG_y
Cuts at: 113 5275 5456 5665 113
Size: 5162 181 209 1725
Fragments arranged by size:
5162 1725 209 181
BsrGI T'GTAC_A
Cuts at: 602 602
Size: 7277
BssSI C'ACGA_G
Cuts at: 5548 5906 5548
Size: 358 6919
BstAPI GCAn_nnn'nTGC
Cuts at: 4654 4654
Size: 7277
BstBI TT'CG_AA
Cuts at: 2652 3411 5638 2652
Size: 759 2227 4291
Fragments arranged by size:
4291 2227 759
BstXI CCAn_nnnn'nTGG
Cuts at: 234 234
Size: 7277
BstYI r'GATC_y
Cuts at: 1 1908 2267 4302 4769 4792 5127 5373 6385
Size: 1907 359 2035 467 23 335 246 1012
Cuts at: 6385 6471 6483 6829 1
Size: 86 12 346 449
Fragments arranged by size:
2035 1907 1012 467 449 359 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1457 1457
Size: 7277
Bsu36I CC'TnA_GG
Cuts at: 884 884
Size: 7277
BtgI C'CryG_G
Cuts at: 728 2013 728
Size: 1285 5992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 431 5227 5408 5571 431
Size: 4796 181 163 2137
Fragments arranged by size:
4796 2137 181 163
BtsI GCAGTG_nn'
Cuts at: 689 1242 3522 4520 689
Size: 553 2280 998 3446
Fragments arranged by size:
3446 2280 998 553
DraI TTT'AAA
Cuts at: 2072 2345 4276 2072
Size: 273 1931 5073
Fragments arranged by size:
5073 1931 273
DrdI GACnn_nn'nnGTC
Cuts at: 4241 4983 5841 4241
Size: 742 858 5677
Fragments arranged by size:
5677 858 742
EaeI y'GGCC_r
Cuts at: 4237 4862 5036 5427 5454 6799 4237
Size: 625 174 391 27 1345 4715
Fragments arranged by size:
4715 1345 625 391 174 27
EagI C'GGCC_G
Cuts at: 4862 4862
Size: 7277
EarI CTCTTCn'nnn_
Cuts at: 399 1556 2107 3392 4106 5300 5510 399
Size: 1157 551 1285 714 1194 210 2166
Fragments arranged by size:
2166 1285 1194 1157 714 551 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5485 5795 5941 7087 5485
Size: 310 146 1146 5675
Fragments arranged by size:
5675 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2439 2691 4289 2439
Size: 252 1598 5427
Fragments arranged by size:
5427 1598 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 716 1076 1615 1771 1954 1960 2107 2128
Size: 499 360 539 156 183 6 147 21
Cuts at: 2128 2557 2677 2872 2924 2971 3416 4154 5102
Size: 429 120 195 52 47 445 738 948
Cuts at: 5102 5534 6281 6549 217
Size: 432 747 268 945
Fragments arranged by size:
948 945 747 738 539 499 445 432
429 360 268 195 183 156 147 120
52 47 21 6
EcoO109I rG'GnC_Cy
Cuts at: 4416 4416
Size: 7277
EcoRI G'AATT_C
Cuts at: 1038 4281 1038
Size: 3243 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 428 721 1489 1741 2343 3592 5296 428
Size: 293 768 252 602 1249 1704 2409
Fragments arranged by size:
2409 1704 1249 768 602 293 252
FspI TGC'GCA
Cuts at: 5058 5058
Size: 7277
HaeII r_GCGC'y
Cuts at: 146 205 1627 2517 4711 4959 5981 6960 146
Size: 59 1422 890 2194 248 1022 979 463
Fragments arranged by size:
2194 1422 1022 979 890 463 248 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 862 894 925 957 1369 1401 3100
Size: 32 625 32 31 32 412 32 1699
Cuts at: 3100 3132 3219 3251 3304 3336 3342 3374 5119
Size: 32 87 32 53 32 6 32 1745
Cuts at: 5119 5149 5151 5181 205
Size: 30 2 30 2301
Fragments arranged by size:
2301 1745 1699 625 412 87 53 32
32 32 32 32 32 32 32 31
30 30 6 2
HincII GTy'rAC
Cuts at: 1707 2559 4246 4316 1707
Size: 852 1687 70 4668
Fragments arranged by size:
4668 1687 852 70
HindIII A'AGCT_T
Cuts at: 469 1310 469
Size: 841 6436
HpaI GTT'AAC
Cuts at: 1707 2559 1707
Size: 852 6425
KasI G'GCGC_C
Cuts at: 142 4707 4955 142
Size: 4565 248 2464
Fragments arranged by size:
4565 2464 248
KpnI G_GTAC'C
Cuts at: 4297 4297
Size: 7277
MfeI C'AATT_G
Cuts at: 2897 3008 2897
Size: 111 7166
MluI A'CGCG_T
Cuts at: 6710 6710
Size: 7277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 840 1299 3756 3834 5948 6132 840
Size: 459 2457 78 2114 184 1985
Fragments arranged by size:
2457 2114 1985 459 184 78
MscI TGG'CCA
Cuts at: 5038 5038
Size: 7277
MslI CAynn'nnrTG
Cuts at: 232 610 746 814 1640 2614 3271 5266 5393
Size: 378 136 68 826 974 657 1995 127
Cuts at: 5393 232
Size: 2116
Fragments arranged by size:
2116 1995 974 826 657 378 136 127
68
MspA1I CmG'CkG
Cuts at: 1329 1890 3462 4388 4702 5062 6075 6320 1329
Size: 561 1572 926 314 360 1013 245 2286
Fragments arranged by size:
2286 1572 1013 926 561 360 314 245
NaeI GCC'GGC
Cuts at: 115 5458 115
Size: 5343 1934
NarI GG'CG_CC
Cuts at: 143 4708 4956 143
Size: 4565 248 2464
Fragments arranged by size:
4565 2464 248
NcoI C'CATG_G
Cuts at: 728 2013 728
Size: 1285 5992
NdeI CA'TA_TG
Cuts at: 3308 4019 3308
Size: 711 6566
NgoMIV G'CCGG_C
Cuts at: 113 5456 113
Size: 5343 1934
NspI r_CATG'y
Cuts at: 624 768 1081 3960 4330 4830 5737 624
Size: 144 313 2879 370 500 907 2164
Fragments arranged by size:
2879 2164 907 500 370 313 144
PasI CC'CwG_GG
Cuts at: 557 557
Size: 7277
PciI A'CATG_T
Cuts at: 3956 5733 3956
Size: 1777 5500
PflMI CCAn_nnn'nTGG
Cuts at: 2610 2610
Size: 7277
PfoI T'CCnGG_A
Cuts at: 2693 2693
Size: 7277
PmeI GTTT'AAAC
Cuts at: 4276 4276
Size: 7277
PmlI CAC'GTG
Cuts at: 812 812
Size: 7277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6416 6448 6416
Size: 32 7245
PsiI TTA'TAA
Cuts at: 2735 2735
Size: 7277
PstI C_TGCA'G
Cuts at: 1842 3425 4324 5009 1842
Size: 1583 899 685 4110
Fragments arranged by size:
4110 1583 899 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7277
PvuII CAG'CTG
Cuts at: 1329 1890 3462 4702 5062 1329
Size: 561 1572 1240 360 3544
Fragments arranged by size:
3544 1572 1240 561 360
RsrII CG'GwC_CG
Cuts at: 5472 5472
Size: 7277
SacI G_AGCT'C
Cuts at: 2441 2693 4291 2441
Size: 252 1598 5427
Fragments arranged by size:
5427 1598 252
SalI G'TCGA_C
Cuts at: 4244 4314 4244
Size: 70 7207
SapI GCTCTTCn'nnn_
Cuts at: 4106 5300 5510 4106
Size: 1194 210 5873
Fragments arranged by size:
5873 1194 210
SbfI CC_TGCA'GG
Cuts at: 4324 4324
Size: 7277
ScaI AGT'ACT
Cuts at: 6794 6794
Size: 7277
SfcI C'TryA_G
Cuts at: 33 361 944 1217 1353 1838 3421 4106 4320
Size: 328 583 273 136 485 1583 685 214
Cuts at: 4320 5005 5998 6189 33
Size: 685 993 191 1121
Fragments arranged by size:
1583 1121 993 685 685 583 485 328
273 214 191 136
SfoI GGC'GCC
Cuts at: 144 4709 4957 144
Size: 4565 248 2464
Fragments arranged by size:
4565 2464 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7277
SmaI CCC'GGG
Cuts at: 4299 4299
Size: 7277
SmlI C'TyrA_G
Cuts at: 3019 3203 4432 5347 5839 6101 3019
Size: 184 1229 915 492 262 4195
Fragments arranged by size:
4195 1229 915 492 262 184
SpeI A'CTAG_T
Cuts at: 67 3043 67
Size: 2976 4301
SphI G_CATG'C
Cuts at: 4330 4830 4330
Size: 500 6777
SspI AAT'ATT
Cuts at: 2914 2914
Size: 7277
StyI C'CwwG_G
Cuts at: 263 728 2013 3781 4411 4765 263
Size: 465 1285 1768 630 354 2775
Fragments arranged by size:
2775 1768 1285 630 465 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 549 4860 4993 5452 5661 549
Size: 4311 133 459 209 2165
Fragments arranged by size:
4311 2165 459 209 133
TatI w'GTAC_w
Cuts at: 602 617 6792 602
Size: 15 6175 1087
Fragments arranged by size:
6175 1087 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1034 1066 1498 1530 6794 6826 1034
Size: 32 432 32 5264 32 1485
Fragments arranged by size:
5264 1485 432 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5074 5074
Size: 7277
XbaI T'CTAG_A
Cuts at: 1986 4308 1986
Size: 2322 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1500 2310 3696 4772 1500
Size: 810 1386 1076 4005
Fragments arranged by size:
4005 1386 1076 810
XmaI C'CCGG_G
Cuts at: 4297 4297
Size: 7277
XmnI GAAnn'nnTTC
Cuts at: 701 701
Size: 7277
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI NheI
NotI NruI NsiI PacI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI