Restriction map for
pF1KA0621
(Circular) (Six-base) MAPSORT of: pF1KA0621.seq Check: 7089 from: 1 to: 5279
pF1KA0621 5279 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1130 1217 2317 1130
Size: 87 1100 4092
Fragments arranged by size:
4092 1100 87
Acc65I G'GTAC_C
Cuts at: 1008 2295 1008
Size: 1287 3992
AclI AA'CG_TT
Cuts at: 5119 5119
Size: 5279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 571 1260 1486 3104 3536 4283 4551 254
Size: 317 689 226 1618 432 747 268 982
Fragments arranged by size:
1618 982 747 689 432 317 268 226
AfeI AGC'GCT
Cuts at: 122 122
Size: 5279
AflIII A'CryG_T
Cuts at: 2631 3735 4712 2631
Size: 1104 977 3198
Fragments arranged by size:
3198 1104 977
AgeI A'CCGG_T
Cuts at: 3667 3667
Size: 5279
AhdI GACnn_n'nnGTC
Cuts at: 237 896 1023 237
Size: 659 127 4493
Fragments arranged by size:
4493 659 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 392 392
Size: 5279
AlwNI CAG_nnn'CTG
Cuts at: 137 2584 4151 137
Size: 2447 1567 1265
Fragments arranged by size:
2447 1567 1265
ApaLI G'TGCA_C
Cuts at: 4049 4049
Size: 5279
ApoI r'AATT_y
Cuts at: 208 261 304 310 555 1668 1759 2283 4632
Size: 53 43 6 245 1113 91 524 2349
Cuts at: 4632 4888 208
Size: 256 599
Fragments arranged by size:
2349 1113 599 524 256 245 91 53
43 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 5279
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5279
AvaI C'yCGr_G
Cuts at: 124 1559 2299 124
Size: 1435 740 3104
Fragments arranged by size:
3104 1435 740
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 992 1025 992
Size: 33 5246
BamHI G'GATC_C
Cuts at: 2304 2304
Size: 5279
BanI G'GyrC_C
Cuts at: 1008 2295 2709 2957 2992 1008
Size: 1287 414 248 35 3295
Fragments arranged by size:
3295 1287 414 248 35
BanII G_rGCy'C
Cuts at: 116 224 2293 3323 116
Size: 108 2069 1030 2072
Fragments arranged by size:
2072 2069 1030 108
BbeI G_GCGC'C
Cuts at: 2713 2961 2713
Size: 248 5031
BbsI GAAGACnn'nnnn_
Cuts at: 260 554 645 1474 2169 260
Size: 294 91 829 695 3370
Fragments arranged by size:
3370 829 695 294 91
BbvCI CC'TCA_GC
Cuts at: 1717 1717
Size: 5279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1840 1874 2160 2194 1840
Size: 34 286 34 4925
Fragments arranged by size:
4925 286 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 404 648 910 3177 3938 5279 404
Size: 244 262 2267 761 1341 404
Fragments arranged by size:
2267 1341 761 404 262 244
BclI T'GATC_A
Cuts at: 2799 2799
Size: 5279
BfrBI ATG'CAT
Cuts at: 1295 1295
Size: 5279
BglII A'GATC_T
Cuts at: 2794 4831 2794
Size: 2037 3242
BlpI GC'TnA_GC
Cuts at: 2391 2391
Size: 5279
Bme1580I G_kGCm'C
Cuts at: 1823 2904 2997 4053 1823
Size: 1081 93 1056 3049
Fragments arranged by size:
3049 1081 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2491 2572 2907 5051 2491
Size: 81 335 2144 2719
Fragments arranged by size:
2719 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 187 3646 187
Size: 3459 1820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1344 1564 1956 2031 1344
Size: 220 392 75 4592
Fragments arranged by size:
4592 392 220 75
Bpu10I CC'TnA_GC
Cuts at: 1717 1717
Size: 5279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 241 901 2455 3334 3826 4124 157
Size: 84 660 1554 879 492 298 1312
Fragments arranged by size:
1554 1312 879 660 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 1569 4983 1569
Size: 3414 1865
BsaAI yAC'GTr
Cuts at: 927 2262 2632 927
Size: 1335 370 3574
Fragments arranged by size:
3574 1335 370
BsaBI GATnn'nnATC
Cuts at: 6 2793 4830 6
Size: 2787 2037 455
Fragments arranged by size:
2787 2037 455
BsaHI Gr'CG_yC
Cuts at: 2710 2958 3660 5156 2710
Size: 248 702 1496 2833
Fragments arranged by size:
2833 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1175 2105 2468 2989 3667 3941 4088 4499 1175
Size: 930 363 521 678 274 147 411 1955
Fragments arranged by size:
1955 930 678 521 411 363 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2029 2059 2029
Size: 30 5249
BseYI C'CCAG_C
Cuts at: 117 1844 2704 3462 4039 4535 117
Size: 1727 860 758 577 496 861
Fragments arranged by size:
1727 861 860 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 949 1056 1387 1834 682
Size: 267 107 331 447 4127
Fragments arranged by size:
4127 447 331 267 107
BsiEI CG_ry'CG
Cuts at: 104 2821 2867 4075 104
Size: 2717 46 1208 1308
Fragments arranged by size:
2717 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 224 443 887 1464 2293 3071 3261 4053 224
Size: 219 444 577 829 778 190 792 1450
Fragments arranged by size:
1450 829 792 778 577 444 219 190
BsmI GAATG_Cn'
Cuts at: 432 1487 432
Size: 1055 4224
BsmBI CGTCTCn'nnnn_
Cuts at: 160 160
Size: 5279
Bsp1286I G_dGCh'C
Cuts at: 116 224 443 887 1464 1823 2293 2904 2997
Size: 108 219 444 577 359 470 611 93
Cuts at: 2997 3071 3261 3323 4053 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 577 470 444 359 219
190 108 93 74 62
BspEI T'CCGG_A
Cuts at: 2105 4499 2105
Size: 2394 2885
BspHI T'CATG_A
Cuts at: 1499 1628 4455 1499
Size: 129 2827 2323
Fragments arranged by size:
2827 2323 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2329 2543 2845 3226 2329
Size: 214 302 381 4382
Fragments arranged by size:
4382 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3571 3625 5109 5274 50
Size: 3521 54 1484 165 55
Fragments arranged by size:
3521 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3191 3191
Size: 5279
BsrFI r'CCGG_y
Cuts at: 3277 3458 3667 3277
Size: 181 209 4889
Fragments arranged by size:
4889 209 181
BsrGI T'GTAC_A
Cuts at: 973 973
Size: 5279
BssHII G'CGCG_C
Cuts at: 251 251
Size: 5279
BssSI C'ACGA_G
Cuts at: 3550 3908 3550
Size: 358 4921
BstAPI GCAn_nnn'nTGC
Cuts at: 1374 1648 2656 1374
Size: 274 1008 3997
Fragments arranged by size:
3997 1008 274
BstBI TT'CG_AA
Cuts at: 465 3640 465
Size: 3175 2104
BstYI r'GATC_y
Cuts at: 1 1781 2304 2771 2794 3129 3375 4387 4473
Size: 1780 523 467 23 335 246 1012 86
Cuts at: 4473 4485 4831 1
Size: 12 346 449
Fragments arranged by size:
1780 1012 523 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 388 1724 388
Size: 1336 3943
BtgI C'CryG_G
Cuts at: 107 744 908 1004 1196 107
Size: 637 164 96 192 4190
Fragments arranged by size:
4190 637 192 164 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3229 3410 3573 3229
Size: 181 163 4935
Fragments arranged by size:
4935 181 163
BtsI GCAGTG_nn'
Cuts at: 1878 2522 1878
Size: 644 4635
DraI TTT'AAA
Cuts at: 309 2278 309
Size: 1969 3310
DrdI GACnn_nn'nnGTC
Cuts at: 174 2985 3843 174
Size: 2811 858 1610
Fragments arranged by size:
2811 1610 858
EaeI y'GGCC_r
Cuts at: 758 1203 2864 3038 3429 3456 4801 758
Size: 445 1661 174 391 27 1345 1236
Fragments arranged by size:
1661 1345 1236 445 391 174 27
EagI C'GGCC_G
Cuts at: 2864 2864
Size: 5279
EarI CTCTTCn'nnn_
Cuts at: 733 1096 1163 1861 1921 3302 3512 733
Size: 363 67 698 60 1381 210 2500
Fragments arranged by size:
2500 1381 698 363 210 67 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1095 3487 3797 3943 5089 1095
Size: 2392 310 146 1146 1285
Fragments arranged by size:
2392 1285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 222 2291 222
Size: 2069 3210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 571 1260 1344 1486 1564 1956 2031 3104
Size: 317 689 84 142 78 392 75 1073
Cuts at: 3104 3536 4283 4551 254
Size: 432 747 268 982
Fragments arranged by size:
1073 982 747 689 432 392 317 268
142 84 78 75
EcoO109I rG'GnC_Cy
Cuts at: 1904 2418 1904
Size: 514 4765
EcoRI G'AATT_C
Cuts at: 2283 2283
Size: 5279
EcoRV GAT'ATC
Cuts at: 1425 1425
Size: 5279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3298 3298
Size: 5279
FspI TGC'GCA
Cuts at: 1256 3060 1256
Size: 1804 3475
HaeII r_GCGC'y
Cuts at: 124 2713 2961 3983 4962 124
Size: 2589 248 1022 979 441
Fragments arranged by size:
2589 1022 979 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 882 914 1009 1041 3121 3151 3153
Size: 32 627 32 95 32 2080 30 2
Cuts at: 3153 3183 223
Size: 30 2319
Fragments arranged by size:
2319 2080 627 95 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 791 1356 1934 2318 791
Size: 565 578 384 3752
Fragments arranged by size:
3752 578 565 384
HindIII A'AGCT_T
Cuts at: 1157 1157
Size: 5279
HpaI GTT'AAC
Cuts at: 791 791
Size: 5279
KasI G'GCGC_C
Cuts at: 2709 2957 2709
Size: 248 5031
KpnI G_GTAC'C
Cuts at: 1012 2299 1012
Size: 1287 3992
MluI A'CGCG_T
Cuts at: 4712 4712
Size: 5279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1241 2195 3950 4134 1145
Size: 96 954 1755 184 2290
Fragments arranged by size:
2290 1755 954 184 96
MscI TGG'CCA
Cuts at: 760 3040 760
Size: 2280 2999
MslI CAynn'nnrTG
Cuts at: 629 3268 3395 629
Size: 2639 127 2513
Fragments arranged by size:
2639 2513 127
MspA1I CmG'CkG
Cuts at: 1623 1848 2390 2704 3064 4077 4322 1623
Size: 225 542 314 360 1013 245 2580
Fragments arranged by size:
2580 1013 542 360 314 245 225
NaeI GCC'GGC
Cuts at: 3460 3460
Size: 5279
NarI GG'CG_CC
Cuts at: 2710 2958 2710
Size: 248 5031
NcoI C'CATG_G
Cuts at: 107 744 908 1004 1196 107
Size: 637 164 96 192 4190
Fragments arranged by size:
4190 637 192 164 96
NgoMIV G'CCGG_C
Cuts at: 3458 3458
Size: 5279
NsiI A_TGCA'T
Cuts at: 1297 1297
Size: 5279
NspI r_CATG'y
Cuts at: 2332 2832 3739 2332
Size: 500 907 3872
Fragments arranged by size:
3872 907 500
PciI A'CATG_T
Cuts at: 3735 3735
Size: 5279
PflMI CCAn_nnn'nTGG
Cuts at: 1305 1305
Size: 5279
PfoI T'CCnGG_A
Cuts at: 1488 1488
Size: 5279
PmeI GTTT'AAAC
Cuts at: 2278 2278
Size: 5279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 5247
PspXI vC'TCGA_Gb
Cuts at: 124 124
Size: 5279
PstI C_TGCA'G
Cuts at: 1897 2326 3011 1897
Size: 429 685 4165
Fragments arranged by size:
4165 685 429
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5279
PvuII CAG'CTG
Cuts at: 1623 1848 2704 3064 1623
Size: 225 856 360 3838
Fragments arranged by size:
3838 856 360 225
RsrII CG'GwC_CG
Cuts at: 3474 3474
Size: 5279
SacI G_AGCT'C
Cuts at: 224 2293 224
Size: 2069 3210
SalI G'TCGA_C
Cuts at: 2316 2316
Size: 5279
SapI GCTCTTCn'nnn_
Cuts at: 1861 1921 3302 3512 1861
Size: 60 1381 210 3628
Fragments arranged by size:
3628 1381 210 60
SbfI CC_TGCA'GG
Cuts at: 2326 2326
Size: 5279
ScaI AGT'ACT
Cuts at: 4796 4796
Size: 5279
SexAI A'CCwGG_T
Cuts at: 611 611
Size: 5279
SfcI C'TryA_G
Cuts at: 33 1893 2322 3007 4000 4191 33
Size: 1860 429 685 993 191 1121
Fragments arranged by size:
1860 1121 993 685 429 191
SfoI GGC'GCC
Cuts at: 2711 2959 2711
Size: 248 5031
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5279
SmaI CCC'GGG
Cuts at: 2301 2301
Size: 5279
SmlI C'TyrA_G
Cuts at: 124 172 256 880 2434 3349 3841 4103 124
Size: 48 84 624 1554 915 492 262 1300
Fragments arranged by size:
1554 1300 915 624 492 262 84 48
SpeI A'CTAG_T
Cuts at: 67 1456 67
Size: 1389 3890
SphI G_CATG'C
Cuts at: 2332 2832 2332
Size: 500 4779
StuI AGG'CCT
Cuts at: 1722 1722
Size: 5279
StyI C'CwwG_G
Cuts at: 107 452 488 744 761 908 1004 1196 1689
Size: 345 36 256 17 147 96 192 493
Cuts at: 1689 2413 2767 107
Size: 724 354 2619
Fragments arranged by size:
2619 724 493 354 345 256 192 147
96 36 17
SwaI ATTT'AAAT
Cuts at: 309 309
Size: 5279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 948 2207 2862 2995 3454 3663 948
Size: 1259 655 133 459 209 2564
Fragments arranged by size:
2564 1259 655 459 209 133
TatI w'GTAC_w
Cuts at: 973 4794 973
Size: 3821 1458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4796 4828 4796
Size: 32 5247
Tth111I GACn'n_nGTC
Cuts at: 613 3076 613
Size: 2463 2816
XbaI T'CTAG_A
Cuts at: 2310 2310
Size: 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2774 2774
Size: 5279
XhoI C'TCGA_G
Cuts at: 124 124
Size: 5279
XmaI C'CCGG_G
Cuts at: 2299 2299
Size: 5279
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BglI BmgBI BmtI BseRI BsiWI BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI MfeI NdeI
NheI NotI NruI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI XmnI ZraI