Restriction map for
pF1KA0622
(Circular) (Six-base) MAPSORT of: pF1KA0622.seq Check: 7962 from: 1 to: 7502
pF1KA0622 7502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 129 129
Size: 7502
AccI GT'mk_AC
Cuts at: 4271 4540 4271
Size: 269 7233
Acc65I G'GTAC_C
Cuts at: 4518 4518
Size: 7502
AclI AA'CG_TT
Cuts at: 914 7342 914
Size: 6428 1074
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1349 1641 1747 2231 2668 3428 5327 5759
Size: 396 292 106 484 437 760 1899 432
Cuts at: 5759 6112 6774 953
Size: 353 662 1681
Fragments arranged by size:
1899 1681 760 662 484 437 432 396
353 292 106
AfeI AGC'GCT
Cuts at: 2512 2512
Size: 7502
AflII C'TTAA_G
Cuts at: 2830 2830
Size: 7502
AflIII A'CryG_T
Cuts at: 696 708 4854 6654 6935 696
Size: 12 4146 1800 281 1263
Fragments arranged by size:
4146 1800 1263 281 12
AhdI GACnn_n'nnGTC
Cuts at: 327 1248 327
Size: 921 6581
AleI CACnn'nnGTG
Cuts at: 3262 3262
Size: 7502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 400 628 4174 400
Size: 228 3546 3728
Fragments arranged by size:
3728 3546 228
AlwNI CAG_nnn'CTG
Cuts at: 4807 6245 4807
Size: 1438 6064
ApaI G_GGCC'C
Cuts at: 4446 4446
Size: 7502
ApaLI G'TGCA_C
Cuts at: 6340 6340
Size: 7502
ApoI r'AATT_y
Cuts at: 525 686 772 825 1504 3169 3246 3998 4506
Size: 161 86 53 679 1665 77 752 508
Cuts at: 4506 6855 7111 525
Size: 2349 256 916
Fragments arranged by size:
2349 1665 916 752 679 508 256 161
86 77 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 7502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
AvaI C'yCGr_G
Cuts at: 536 3651 4522 536
Size: 3115 871 3516
Fragments arranged by size:
3516 3115 871
AvrII C'CTAG_G
Cuts at: 1435 1435
Size: 7502
BamHI G'GATC_C
Cuts at: 958 2024 3027 4527 958
Size: 1066 1003 1500 3933
Fragments arranged by size:
3933 1500 1066 1003
BanI G'GyrC_C
Cuts at: 3187 3214 4518 4932 5180 5215 3187
Size: 27 1304 414 248 35 5474
Fragments arranged by size:
5474 1304 414 248 35 27
BanII G_rGCy'C
Cuts at: 2218 3271 3855 4446 4516 5546 2218
Size: 1053 584 591 70 1030 4174
Fragments arranged by size:
4174 1053 1030 591 584 70
BbeI G_GCGC'C
Cuts at: 4936 5184 4936
Size: 248 7254
BbsI GAAGACnn'nnnn_
Cuts at: 959 2237 3890 4060 4379 959
Size: 1278 1653 170 319 4082
Fragments arranged by size:
4082 1653 1278 319 170
BbvCI CC'TCA_GC
Cuts at: 4103 4103
Size: 7502
BciVI GTATCCnnnnn_n'
Cuts at: 5400 6456 7502 5400
Size: 1056 1046 5400
Fragments arranged by size:
5400 1056 1046
BclI T'GATC_A
Cuts at: 2916 5022 2916
Size: 2106 5396
BglII A'GATC_T
Cuts at: 720 1153 2131 2394 2696 3477 5017 7054 720
Size: 433 978 263 302 781 1540 2037 1168
Fragments arranged by size:
2037 1540 1168 978 781 433 302 263
BlpI GC'TnA_GC
Cuts at: 4614 4614
Size: 7502
Bme1580I G_kGCm'C
Cuts at: 1966 3773 4446 5127 5220 6344 1966
Size: 1807 673 681 93 1124 3124
Fragments arranged by size:
3124 1807 1124 681 673 93
BmgBI CAC'GTC
Cuts at: 3114 3768 3114
Size: 654 6848
BmrI ACTGGGnnnn_n'
Cuts at: 477 1647 1892 2219 4714 4795 5130 7274 477
Size: 1170 245 327 2495 81 335 2144 705
Fragments arranged by size:
2495 2144 1170 705 335 327 245 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5869 5869
Size: 7502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1876 1982 2508 3880 3958 602
Size: 1274 106 526 1372 78 4146
Fragments arranged by size:
4146 1372 1274 526 106 78
Bpu10I CC'TnA_GC
Cuts at: 1238 4103 1238
Size: 2865 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1087 1769 2478 2959 3226 3853 4678 5557
Size: 840 682 709 481 267 627 825 879
Cuts at: 5557 6271 6569 247
Size: 714 298 1180
Fragments arranged by size:
1180 879 840 825 714 709 682 627
481 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 1055 1256 3934 7206 1055
Size: 201 2678 3272 1351
Fragments arranged by size:
3272 2678 1351 201
BsaAI yAC'GTr
Cuts at: 489 3430 4855 489
Size: 2941 1425 3136
Fragments arranged by size:
3136 2941 1425
BsaBI GATnn'nnATC
Cuts at: 6 2130 5016 7053 6
Size: 2124 2886 2037 455
Fragments arranged by size:
2886 2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 1969 3295 4933 5181 5883 7379 1969
Size: 1326 1638 248 702 1496 2092
Fragments arranged by size:
2092 1638 1496 1326 702 248
BsaWI w'CCGG_w
Cuts at: 4691 5212 5890 6301 6448 6722 4691
Size: 521 678 411 147 274 5471
Fragments arranged by size:
5471 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 695 725 2949 2979 3621 3651 4060 4090 4099
Size: 30 2224 30 642 30 409 30 9
Cuts at: 4099 4129 4453 4483 695
Size: 30 324 30 3714
Fragments arranged by size:
3714 2224 642 409 324 30 30 30
30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1941 2208 2451 2454 3919 4120 4461 4464 1941
Size: 267 243 3 1465 201 341 3 4979
Fragments arranged by size:
4979 1465 341 267 243 201 3 3
BseYI C'CCAG_C
Cuts at: 312 2120 3632 4927 5685 6350 6758 312
Size: 1808 1512 1295 758 665 408 1056
Fragments arranged by size:
1808 1512 1295 1056 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3214 3214
Size: 7502
BsiEI CG_ry'CG
Cuts at: 104 4184 5044 5090 6320 104
Size: 4080 860 46 1230 1286
Fragments arranged by size:
4080 1286 1230 860 46
BsiHKAI G_wGCw'C
Cuts at: 588 1676 2386 3911 3975 4163 4516 5294 5484
Size: 1088 710 1525 64 188 353 778 190
Cuts at: 5484 6344 588
Size: 860 1746
Fragments arranged by size:
1746 1525 1088 860 778 710 353 190
188 64
BsmI GAATG_Cn'
Cuts at: 506 2370 2537 506
Size: 1864 167 5471
Fragments arranged by size:
5471 1864 167
BsmBI CGTCTCn'nnnn_
Cuts at: 3758 3758
Size: 7502
Bsp1286I G_dGCh'C
Cuts at: 588 1676 1966 2218 2386 3271 3773 3855 3911
Size: 1088 290 252 168 885 502 82 56
Cuts at: 3911 3975 4163 4446 4516 5127 5220 5294 5484
Size: 64 188 283 70 611 93 74 190
Cuts at: 5484 5546 6344 588
Size: 62 798 1746
Fragments arranged by size:
1746 1088 885 798 611 502 290 283
252 190 188 168 93 82 74 70
64 62 56
BspHI T'CATG_A
Cuts at: 5934 5934
Size: 7502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 129 162 1253 4552 4766 5068 5449 129
Size: 33 1091 3299 214 302 381 2182
Fragments arranged by size:
3299 2182 1091 381 302 214 33
BsrBI CCG'CTC
Cuts at: 50 1769 4099 5794 5848 7332 7497 50
Size: 1719 2330 1695 54 1484 165 55
Fragments arranged by size:
2330 1719 1695 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4252 5414 4252
Size: 1162 6340
BsrFI r'CCGG_y
Cuts at: 935 2159 5500 5681 935
Size: 1224 3341 181 2756
Fragments arranged by size:
3341 2756 1224 181
BssHII G'CGCG_C
Cuts at: 3642 3642
Size: 7502
BssSI C'ACGA_G
Cuts at: 2222 5773 6481 2222
Size: 3551 708 3243
Fragments arranged by size:
3551 3243 708
BstAPI GCAn_nnn'nTGC
Cuts at: 144 1933 4879 144
Size: 1789 2946 2767
Fragments arranged by size:
2946 2767 1789
BstBI TT'CG_AA
Cuts at: 3465 5863 3465
Size: 2398 5104
BstEII G'GTnAC_C
Cuts at: 339 339
Size: 7502
BstXI CCAn_nnnn'nTGG
Cuts at: 793 1380 3024 793
Size: 587 1644 5271
Fragments arranged by size:
5271 1644 587
BstYI r'GATC_y
Cuts at: 1 639 720 958 1153 2024 2131 2394 2696
Size: 638 81 238 195 871 107 263 302
Cuts at: 2696 2857 3027 3477 4527 4994 5017 5352 5598
Size: 161 170 450 1050 467 23 335 246
Cuts at: 5598 5904 5916 6002 7054 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1050 871 638 467 450 449 335
306 302 263 246 238 195 170 161
107 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 7502
BtgI C'CryG_G
Cuts at: 107 1320 3354 3658 107
Size: 1213 2034 304 3951
Fragments arranged by size:
3951 2034 1213 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3548 5452 5633 5796 3548
Size: 1904 181 163 5254
Fragments arranged by size:
5254 1904 181 163
BtsI GCAGTG_nn'
Cuts at: 4153 4745 4153
Size: 592 6910
DraI TTT'AAA
Cuts at: 1227 4026 4501 1227
Size: 2799 475 4228
Fragments arranged by size:
4228 2799 475
DrdI GACnn_nn'nnGTC
Cuts at: 5208 6552 5208
Size: 1344 6158
EaeI y'GGCC_r
Cuts at: 177 1382 2334 3014 3288 4124 4181 5087 5261
Size: 1205 952 680 274 836 57 906 174
Cuts at: 5261 5652 5679 7024 177
Size: 391 27 1345 655
Fragments arranged by size:
1345 1205 952 906 836 680 655 391
274 174 57 27
EagI C'GGCC_G
Cuts at: 4181 5087 4181
Size: 906 6596
EarI CTCTTCn'nnn_
Cuts at: 998 2644 5525 5735 998
Size: 1646 2881 210 2765
Fragments arranged by size:
2881 2765 1646 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 321 5710 6452 6598 7312 321
Size: 5389 742 146 714 511
Fragments arranged by size:
5389 742 714 511 146
EcoICRI GAG'CTC
Cuts at: 4514 4514
Size: 7502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 953 1349 1641 1747 1876 1982 2231 2508
Size: 351 396 292 106 129 106 249 277
Cuts at: 2508 2668 3428 3880 3958 5327 5759 6112 6774
Size: 160 760 452 78 1369 432 353 662
Cuts at: 6774 602
Size: 1330
Fragments arranged by size:
1369 1330 760 662 452 432 396 353
351 292 277 249 160 129 106 106
78
EcoNI CCTnn'n_nnAGG
Cuts at: 136 3727 136
Size: 3591 3911
EcoO109I rG'GnC_Cy
Cuts at: 1242 1886 4442 4641 1242
Size: 644 2556 199 4103
Fragments arranged by size:
4103 2556 644 199
EcoRI G'AATT_C
Cuts at: 3246 4506 3246
Size: 1260 6242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2821 3907 5521 2641
Size: 180 1086 1614 4622
Fragments arranged by size:
4622 1614 1086 180
FspI TGC'GCA
Cuts at: 5283 5283
Size: 7502
HaeII r_GCGC'y
Cuts at: 1497 2514 3545 4936 5184 6414 7185 1497
Size: 1017 1031 1391 248 1230 771 1814
Fragments arranged by size:
1814 1391 1230 1031 1017 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 313 345 450 482 530 562 705
Size: 32 97 32 105 32 48 32 143
Cuts at: 705 737 1234 1266 1756 1782 1788 1814 2688
Size: 32 497 32 490 26 6 26 874
Cuts at: 2688 2720 3511 3543 4099 4131 5344 5374 5376
Size: 32 791 32 556 32 1213 30 2
Cuts at: 5376 5406 184
Size: 30 2280
Fragments arranged by size:
2280 1213 874 791 556 497 490 143
105 97 48 32 32 32 32 32
32 32 32 32 30 30 26 26
6 2
HincII GTy'rAC
Cuts at: 1877 4272 4541 1877
Size: 2395 269 4838
Fragments arranged by size:
4838 2395 269
KasI G'GCGC_C
Cuts at: 4932 5180 4932
Size: 248 7254
KpnI G_GTAC'C
Cuts at: 4522 4522
Size: 7502
MluI A'CGCG_T
Cuts at: 6935 6935
Size: 7502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 897 2002 3055 3842 6261 6445 897
Size: 1105 1053 787 2419 184 1954
Fragments arranged by size:
2419 1954 1105 1053 787 184
MscI TGG'CCA
Cuts at: 179 1384 2336 3016 4126 5263 179
Size: 1205 952 680 1110 1137 2418
Fragments arranged by size:
2418 1205 1137 1110 952 680
MslI CAynn'nnrTG
Cuts at: 621 2707 2786 2935 3262 4156 4213 5491 5618
Size: 2086 79 149 327 894 57 1278 127
Cuts at: 5618 621
Size: 2505
Fragments arranged by size:
2505 2086 1278 894 327 149 127 79
57
MspA1I CmG'CkG
Cuts at: 973 1806 3660 4613 4927 5287 6071 6316 973
Size: 833 1854 953 314 360 784 245 2159
Fragments arranged by size:
2159 1854 953 833 784 360 314 245
NaeI GCC'GGC
Cuts at: 937 5683 937
Size: 4746 2756
NarI GG'CG_CC
Cuts at: 4933 5181 4933
Size: 248 7254
NcoI C'CATG_G
Cuts at: 107 1320 3354 107
Size: 1213 2034 4255
Fragments arranged by size:
4255 2034 1213
NdeI CA'TA_TG
Cuts at: 2517 2517
Size: 7502
NgoMIV G'CCGG_C
Cuts at: 935 5681 935
Size: 4746 2756
NspI r_CATG'y
Cuts at: 700 3378 4555 5055 6658 700
Size: 2678 1177 500 1603 1544
Fragments arranged by size:
2678 1603 1544 1177 500
PciI A'CATG_T
Cuts at: 696 6654 696
Size: 5958 1544
PflMI CCAn_nnn'nTGG
Cuts at: 1689 1689
Size: 7502
PfoI T'CCnGG_A
Cuts at: 2524 3206 2524
Size: 682 6820
PmeI GTTT'AAAC
Cuts at: 4501 4501
Size: 7502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3388 3420 3721 3753 5948 5980 3388
Size: 32 301 32 2195 32 4910
Fragments arranged by size:
4910 2195 301 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1242 1886 1242
Size: 644 6858
PshAI GACnn'nnGTC
Cuts at: 4225 4225
Size: 7502
PsiI TTA'TAA
Cuts at: 2773 2773
Size: 7502
PspOMI G'GGCC_C
Cuts at: 4442 4442
Size: 7502
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2561 2593 2561
Size: 32 7470
PstI C_TGCA'G
Cuts at: 353 1718 1986 2639 4262 4295 4549 5234 353
Size: 1365 268 653 1623 33 254 685 2621
Fragments arranged by size:
2621 1623 1365 685 653 268 254 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7502
PvuII CAG'CTG
Cuts at: 973 4927 5287 973
Size: 3954 360 3188
Fragments arranged by size:
3954 3188 360
RsrII CG'GwC_CG
Cuts at: 5697 5697
Size: 7502
SacI G_AGCT'C
Cuts at: 4516 4516
Size: 7502
SacII CC_GC'GG
Cuts at: 3661 3661
Size: 7502
SalI G'TCGA_C
Cuts at: 4270 4539 4270
Size: 269 7233
SanDI GG'GwC_CC
Cuts at: 1886 1886
Size: 7502
SapI GCTCTTCn'nnn_
Cuts at: 2644 5525 5735 2644
Size: 2881 210 4411
Fragments arranged by size:
4411 2881 210
SbfI CC_TGCA'GG
Cuts at: 4549 4549
Size: 7502
ScaI AGT'ACT
Cuts at: 7019 7019
Size: 7502
SexAI A'CCwGG_T
Cuts at: 2352 3788 2352
Size: 1436 6066
SfcI C'TryA_G
Cuts at: 33 349 1037 1714 1853 1982 2635 3417 4258
Size: 316 688 677 139 129 653 782 841
Cuts at: 4258 4291 4545 5230 6198 6389 33
Size: 33 254 685 968 191 1146
Fragments arranged by size:
1146 968 841 782 688 685 677 653
316 254 191 139 129 33
SfoI GGC'GCC
Cuts at: 4934 5182 4934
Size: 248 7254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
SmaI CCC'GGG
Cuts at: 3653 4524 3653
Size: 871 6631
SmlI C'TyrA_G
Cuts at: 226 536 1066 1784 2493 2830 2974 3241 3868
Size: 310 530 718 709 337 144 267 627
Cuts at: 3868 4657 5572 6286 6548 226
Size: 789 915 714 262 1180
Fragments arranged by size:
1180 915 789 718 714 709 627 530
337 310 267 262 144
SnaBI TAC'GTA
Cuts at: 489 489
Size: 7502
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7502
SphI G_CATG'C
Cuts at: 3378 4555 5055 3378
Size: 1177 500 5825
Fragments arranged by size:
5825 1177 500
SspI AAT'ATT
Cuts at: 1097 1097
Size: 7502
StyI C'CwwG_G
Cuts at: 107 887 954 1320 1435 1688 3354 3891 3945
Size: 780 67 366 115 253 1666 537 54
Cuts at: 3945 4636 4990 107
Size: 691 354 2619
Fragments arranged by size:
2619 1666 780 691 537 366 354 253
115 67 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 270 3354 3646 5085 5218 5677 5886 270
Size: 3084 292 1439 133 459 209 1886
Fragments arranged by size:
3084 1886 1439 459 292 209 133
TatI w'GTAC_w
Cuts at: 1041 2389 7017 1041
Size: 1348 4628 1526
Fragments arranged by size:
4628 1526 1348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 695 727 3621 3653 4060 4092 7019 7051 695
Size: 32 2894 32 407 32 2927 32 1146
Fragments arranged by size:
2927 2894 1146 407 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1884 2241 5299 1884
Size: 357 3058 4087
Fragments arranged by size:
4087 3058 357
XbaI T'CTAG_A
Cuts at: 1843 2886 3073 4533 1843
Size: 1043 187 1460 4812
Fragments arranged by size:
4812 1460 1043 187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4997 4997
Size: 7502
XhoI C'TCGA_G
Cuts at: 536 536
Size: 7502
XmaI C'CCGG_G
Cuts at: 3651 4522 3651
Size: 871 6631
XmnI GAAnn'nnTTC
Cuts at: 2624 2624
Size: 7502
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AloI AscI BaeI BcgI BfrBI BglI
BmtI BsiWI BspEI BsrGI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PmlI PspXI SfiI SgrAI
SrfI StuI SwaI ZraI