Restriction map for
pF1KA0625
(Circular) (Six-base) MAPSORT of: pF1KA0625.seq Check: 1594 from: 1 to: 11144
pF1KA0625 11144 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1999 1999
Size: 11144
AccI GT'mk_AC
Cuts at: 377 1175 6045 7220 8182 377
Size: 798 4870 1175 962 3339
Fragments arranged by size:
4870 3339 1175 962 798
Acc65I G'GTAC_C
Cuts at: 8160 8160
Size: 11144
AclI AA'CG_TT
Cuts at: 10984 10984
Size: 11144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 1442 2231 2807 3389 3512 3638 4135 4472
Size: 172 789 576 582 123 126 497 337
Cuts at: 4472 4637 4835 4943 6615 7699 7753 8969 9401
Size: 165 198 108 1672 1084 54 1216 432
Cuts at: 9401 10148 10416 1270
Size: 747 268 1998
Fragments arranged by size:
1998 1672 1216 1084 789 747 582 576
497 432 337 268 198 172 165 126
123 108 54
AfeI AGC'GCT
Cuts at: 166 166
Size: 11144
AflII C'TTAA_G
Cuts at: 1369 5898 1369
Size: 4529 6615
AflIII A'CryG_T
Cuts at: 115 489 788 1250 2338 2484 7270 7957 8496
Size: 374 299 462 1088 146 4786 687 539
Cuts at: 8496 9600 10577 115
Size: 1104 977 682
Fragments arranged by size:
4786 1104 1088 977 687 682 539 462
374 299 146
AgeI A'CCGG_T
Cuts at: 9532 9532
Size: 11144
AleI CACnn'nnGTG
Cuts at: 1921 1921
Size: 11144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6500 7895 6500
Size: 1395 9749
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7177 7209 7177
Size: 32 11112
AlwNI CAG_nnn'CTG
Cuts at: 1453 3163 4107 4279 4405 7857 8449 10016 1453
Size: 1710 944 172 126 3452 592 1567 2581
Fragments arranged by size:
3452 2581 1710 1567 944 592 172 126
ApaI G_GGCC'C
Cuts at: 5270 5270
Size: 11144
ApaLI G'TGCA_C
Cuts at: 6125 9914 6125
Size: 3789 7355
ApoI r'AATT_y
Cuts at: 193 1474 1812 1894 2547 2770 3559 3925 4254
Size: 1281 338 82 653 223 789 366 329
Cuts at: 4254 5067 5418 5443 5635 6175 6231 6361 8148
Size: 813 351 25 192 540 56 130 1787
Cuts at: 8148 10497 10753 193
Size: 2349 256 584
Fragments arranged by size:
2349 1787 1281 813 789 653 584 540
366 351 338 329 256 223 192 130
82 56 25
AseI AT'TA_AT
Cuts at: 20 4090 6263 20
Size: 4070 2173 4901
Fragments arranged by size:
4901 4070 2173
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11144
AvaI C'yCGr_G
Cuts at: 7788 7945 8164 7788
Size: 157 219 10768
Fragments arranged by size:
10768 219 157
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2582 2615 2582
Size: 33 11111
BamHI G'GATC_C
Cuts at: 957 1035 7776 8169 957
Size: 78 6741 393 3932
Fragments arranged by size:
6741 3932 393 78
BanI G'GyrC_C
Cuts at: 7441 8160 8574 8822 8857 7441
Size: 719 414 248 35 9728
Fragments arranged by size:
9728 719 414 248 35
BanII G_rGCy'C
Cuts at: 886 5270 6405 7795 7994 8158 9188 886
Size: 4384 1135 1390 199 164 1030 2842
Fragments arranged by size:
4384 2842 1390 1135 1030 199 164
BbeI G_GCGC'C
Cuts at: 8578 8826 8578
Size: 248 10896
BbsI GAAGACnn'nnnn_
Cuts at: 218 1999 3767 4203 4499 7168 218
Size: 1781 1768 436 296 2669 4194
Fragments arranged by size:
4194 2669 1781 1768 436 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1152 1186 6389 6423 1152
Size: 34 5203 34 5873
Fragments arranged by size:
5873 5203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4991 9042 9803 11144 4991
Size: 4051 761 1341 4991
Fragments arranged by size:
4991 4051 1341 761
BclI T'GATC_A
Cuts at: 2078 4041 6842 8664 2078
Size: 1963 2801 1822 4558
Fragments arranged by size:
4558 2801 1963 1822
BfrBI ATG'CAT
Cuts at: 2013 4355 6348 6888 7234 2013
Size: 2342 1993 540 346 5923
Fragments arranged by size:
5923 2342 1993 540 346
BglII A'GATC_T
Cuts at: 2258 2850 3681 5601 8659 10696 2258
Size: 592 831 1920 3058 2037 2706
Fragments arranged by size:
3058 2706 2037 1920 831 592
BlpI GC'TnA_GC
Cuts at: 8256 8256
Size: 11144
Bme1580I G_kGCm'C
Cuts at: 5270 6129 8769 8862 9918 5270
Size: 859 2640 93 1056 6496
Fragments arranged by size:
6496 2640 1056 859 93
BmgBI CAC'GTC
Cuts at: 4525 5648 4525
Size: 1123 10021
BmrI ACTGGGnnnn_n'
Cuts at: 422 5478 6253 8356 8437 8772 10916 422
Size: 5056 775 2103 81 335 2144 650
Fragments arranged by size:
5056 2144 2103 775 650 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4581 9511 4581
Size: 4930 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4382 4551 5150 7826 7916 8119 4382
Size: 169 599 2676 90 203 7407
Fragments arranged by size:
7407 2676 599 203 169 90
Bpu10I CC'TnA_GC
Cuts at: 3941 6731 7858 3941
Size: 2790 1127 7227
Fragments arranged by size:
7227 2790 1127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 2999 3217 3250 5280 5681 6616 7486 8320
Size: 2128 218 33 2030 401 935 870 834
Cuts at: 8320 9199 9691 9989 871
Size: 879 492 298 2026
Fragments arranged by size:
2128 2030 2026 935 879 870 834 492
401 298 218 33
BsaI GGTCTCn'nnnn_
Cuts at: 2981 4400 4878 8047 10848 2981
Size: 1419 478 3169 2801 3277
Fragments arranged by size:
3277 3169 2801 1419 478
BsaAI yAC'GTr
Cuts at: 3739 4039 7271 7958 8497 3739
Size: 300 3232 687 539 6386
Fragments arranged by size:
6386 3232 687 539 300
BsaBI GATnn'nnATC
Cuts at: 6 563 6972 8658 10695 6
Size: 557 6409 1686 2037 455
Fragments arranged by size:
6409 2037 1686 557 455
BsaHI Gr'CG_yC
Cuts at: 1996 6513 8575 8823 9525 11021 1996
Size: 4517 2062 248 702 1496 2119
Fragments arranged by size:
4517 2119 2062 1496 702 248
BsaWI w'CCGG_w
Cuts at: 187 1131 8333 8854 9532 9806 9953 10364 187
Size: 944 7202 521 678 274 147 411 967
Fragments arranged by size:
7202 967 944 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3711 3741 5588 5618 6070 6100 6669 6699 7518
Size: 30 1847 30 452 30 569 30 819
Cuts at: 7518 7548 3711
Size: 30 7307
Fragments arranged by size:
7307 1847 819 569 452 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2896 4088 4853 6025 8002 2896
Size: 1192 765 1172 1977 6038
Fragments arranged by size:
6038 1977 1192 1172 765
BseYI C'CCAG_C
Cuts at: 1553 2161 4007 7579 7796 8569 9327 9904 10400
Size: 608 1846 3572 217 773 758 577 496
Cuts at: 10400 1553
Size: 2297
Fragments arranged by size:
3572 2297 1846 773 758 608 577 496
217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 6549 1735
Size: 4814 6330
BsiEI CG_ry'CG
Cuts at: 104 8686 8732 9940 104
Size: 8582 46 1208 1308
Fragments arranged by size:
8582 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 6129 6405 6577 7994 8158 8936 9126 9918
Size: 6014 276 172 1417 164 778 190 792
Cuts at: 9918 115
Size: 1341
Fragments arranged by size:
6014 1417 1341 792 778 276 190 172
164
BsmI GAATG_Cn'
Cuts at: 1677 6072 7234 1677
Size: 4395 1162 5587
Fragments arranged by size:
5587 4395 1162
BsmBI CGTCTCn'nnnn_
Cuts at: 316 4979 316
Size: 4663 6481
Bsp1286I G_dGCh'C
Cuts at: 115 886 5270 6129 6405 6577 7795 7994 8158
Size: 771 4384 859 276 172 1218 199 164
Cuts at: 8158 8769 8862 8936 9126 9188 9918 115
Size: 611 93 74 190 62 730 1341
Fragments arranged by size:
4384 1341 1218 859 771 730 611 276
199 190 172 164 93 74 62
BspEI T'CCGG_A
Cuts at: 10364 10364
Size: 11144
BspHI T'CATG_A
Cuts at: 6155 7704 10320 6155
Size: 1549 2616 6979
Fragments arranged by size:
6979 2616 1549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 8194 8408 8710 9091 8194
Size: 214 302 381 10247
Fragments arranged by size:
10247 381 302 214
BsrBI CCG'CTC
Cuts at: 50 9436 9490 10974 11139 50
Size: 9386 54 1484 165 55
Fragments arranged by size:
9386 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1564 1661 1672 5082 9056 1564
Size: 97 11 3410 3974 3652
Fragments arranged by size:
3974 3652 3410 97 11
BsrFI r'CCGG_y
Cuts at: 9142 9323 9532 9142
Size: 181 209 10754
Fragments arranged by size:
10754 209 181
BsrGI T'GTAC_A
Cuts at: 1698 3475 3793 4300 5851 1698
Size: 1777 318 507 1551 6991
Fragments arranged by size:
6991 1777 1551 507 318
BssSI C'ACGA_G
Cuts at: 6118 7346 9415 9773 6118
Size: 1228 2069 358 7489
Fragments arranged by size:
7489 2069 1228 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1683 8521 1683
Size: 6838 4306
BstBI TT'CG_AA
Cuts at: 7125 9505 7125
Size: 2380 8764
BstXI CCAn_nnnn'nTGG
Cuts at: 603 7804 603
Size: 7201 3943
BstYI r'GATC_y
Cuts at: 1 572 957 1009 1035 1088 1365 1926 2258
Size: 571 385 52 26 53 277 561 332
Cuts at: 2258 2850 3621 3681 5601 6726 7776 8169 8636
Size: 592 771 60 1920 1125 1050 393 467
Cuts at: 8636 8659 8994 9240 10252 10338 10350 10696 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1920 1125 1050 1012 771 592 571 561
467 449 393 385 346 335 332 277
246 86 60 53 52 26 23 12
BstZ17I GTA'TAC
Cuts at: 1176 1176
Size: 11144
BtgI C'CryG_G
Cuts at: 863 3135 3538 6609 863
Size: 2272 403 3071 5398
Fragments arranged by size:
5398 3071 2272 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3044 6358 6682 9094 9275 9438 3044
Size: 3314 324 2412 181 163 4750
Fragments arranged by size:
4750 3314 2412 324 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 4578 7490 8387 1598
Size: 2980 2912 897 4355
Fragments arranged by size:
4355 2980 2912 897
ClaI AT'CG_AT
Cuts at: 913 913
Size: 11144
DraI TTT'AAA
Cuts at: 699 2408 2847 3702 5720 8143 699
Size: 1709 439 855 2018 2423 3700
Fragments arranged by size:
3700 2423 2018 1709 855 439
DraIII CAC_nnn'GTG
Cuts at: 4751 4751
Size: 11144
DrdI GACnn_nn'nnGTC
Cuts at: 8850 9708 8850
Size: 858 10286
EaeI y'GGCC_r
Cuts at: 967 5711 6982 8729 8903 9294 9321 10666 967
Size: 4744 1271 1747 174 391 27 1345 1445
Fragments arranged by size:
4744 1747 1445 1345 1271 391 174 27
EagI C'GGCC_G
Cuts at: 8729 8729
Size: 11144
EarI CTCTTCn'nnn_
Cuts at: 452 2268 2503 5561 6815 6910 7369 7729 7979
Size: 1816 235 3058 1254 95 459 360 250
Cuts at: 7979 9167 9377 452
Size: 1188 210 2219
Fragments arranged by size:
3058 2219 1816 1254 1188 459 360 250
235 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9352 9662 9808 10954 9352
Size: 310 146 1146 9542
Fragments arranged by size:
9542 1146 310 146
EcoICRI GAG'CTC
Cuts at: 6403 7992 8156 6403
Size: 1589 164 9391
Fragments arranged by size:
9391 1589 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 1442 2231 2807 3389 3512 3638 4135 4382
Size: 172 789 576 582 123 126 497 247
Cuts at: 4382 4472 4551 4637 4835 4943 5150 6615 7699
Size: 90 79 86 198 108 207 1465 1084
Cuts at: 7699 7753 7826 7916 8119 8969 9401 10148 10416
Size: 54 73 90 203 850 432 747 268
Cuts at: 10416 1270
Size: 1998
Fragments arranged by size:
1998 1465 1084 850 789 747 582 576
497 432 268 247 207 203 198 172
126 123 108 90 90 86 79 73
54
EcoNI CCTnn'n_nnAGG
Cuts at: 757 3976 6005 757
Size: 3219 2029 5896
Fragments arranged by size:
5896 3219 2029
EcoO109I rG'GnC_Cy
Cuts at: 2934 3982 5267 7385 7683 7719 7854 8283 2934
Size: 1048 1285 2118 298 36 135 429 5795
Fragments arranged by size:
5795 2118 1285 1048 429 298 135 36
EcoRI G'AATT_C
Cuts at: 4254 5067 6175 8148 4254
Size: 813 1108 1973 7250
Fragments arranged by size:
7250 1973 1108 813
EcoRV GAT'ATC
Cuts at: 1661 1661
Size: 11144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 703 1812 4786 9163 703
Size: 1109 2974 4377 2684
Fragments arranged by size:
4377 2974 2684 1109
FspI TGC'GCA
Cuts at: 8925 8925
Size: 11144
HaeII r_GCGC'y
Cuts at: 168 8578 8826 9848 10827 168
Size: 8410 248 1022 979 485
Fragments arranged by size:
8410 1022 979 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 984 1001 1016 1033 1612 1644 1713 1745 2818
Size: 17 15 17 579 32 69 32 1073
Cuts at: 2818 2850 3225 3257 3297 3329 3472 3504 3646
Size: 32 375 32 40 32 143 32 142
Cuts at: 3646 3678 3711 3743 6070 6102 6669 6701 7027
Size: 32 33 32 2327 32 567 32 326
Cuts at: 7027 7059 7319 7351 8986 9016 9018 9048 984
Size: 32 260 32 1635 30 2 30 3080
Fragments arranged by size:
3080 2327 1635 1073 579 567 375 326
260 143 142 69 40 33 32 32
32 32 32 32 32 32 32 32
32 32 30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 495 1027 1835 2348 8183 495
Size: 532 808 513 5835 3456
Fragments arranged by size:
5835 3456 808 532 513
HindIII A'AGCT_T
Cuts at: 433 1588 2024 3781 7090 8131 433
Size: 1155 436 1757 3309 1041 3446
Fragments arranged by size:
3446 3309 1757 1155 1041 436
HpaI GTT'AAC
Cuts at: 495 495
Size: 11144
KasI G'GCGC_C
Cuts at: 8574 8822 8574
Size: 248 10896
KpnI G_GTAC'C
Cuts at: 8164 8164
Size: 11144
MfeI C'AATT_G
Cuts at: 537 2754 7301 537
Size: 2217 4547 4380
Fragments arranged by size:
4547 4380 2217
MluI A'CGCG_T
Cuts at: 10577 10577
Size: 11144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 502 1116 3102 4038 4540 4709 5132 5782
Size: 302 614 1986 936 502 169 423 650
Cuts at: 5782 6769 6993 9815 9999 200
Size: 987 224 2822 184 1345
Fragments arranged by size:
2822 1986 1345 987 936 650 614 502
423 302 224 184 169
MscI TGG'CCA
Cuts at: 969 6984 8905 969
Size: 6015 1921 3208
Fragments arranged by size:
6015 3208 1921
MslI CAynn'nnrTG
Cuts at: 488 596 791 1332 1908 1921 2780 3848 5324
Size: 108 195 541 576 13 859 1068 1476
Cuts at: 5324 5391 6757 9133 9260 488
Size: 67 1366 2376 127 2372
Fragments arranged by size:
2376 2372 1476 1366 1068 859 576 541
195 127 108 67 13
MspA1I CmG'CkG
Cuts at: 1719 3066 3958 4011 6142 6378 6635 7416 7879
Size: 1347 892 53 2131 236 257 781 463
Cuts at: 7879 7936 8255 8569 8929 9942 10187 1719
Size: 57 319 314 360 1013 245 2676
Fragments arranged by size:
2676 2131 1347 1013 892 781 463 360
319 314 257 245 236 57 53
NaeI GCC'GGC
Cuts at: 9325 9325
Size: 11144
NarI GG'CG_CC
Cuts at: 8575 8823 8575
Size: 248 10896
NcoI C'CATG_G
Cuts at: 863 863
Size: 11144
NdeI CA'TA_TG
Cuts at: 3996 5943 6898 3996
Size: 1947 955 8242
Fragments arranged by size:
8242 1947 955
NgoMIV G'CCGG_C
Cuts at: 9323 9323
Size: 11144
NsiI A_TGCA'T
Cuts at: 2015 4357 6350 6890 7236 2015
Size: 2342 1993 540 346 5923
Fragments arranged by size:
5923 2342 1993 540 346
NspI r_CATG'y
Cuts at: 119 792 837 1254 1311 1943 2488 3256 4359
Size: 673 45 417 57 632 545 768 1103
Cuts at: 4359 8197 8697 9604 119
Size: 3838 500 907 1659
Fragments arranged by size:
3838 1659 1103 907 768 673 632 545
500 417 57 45
PasI CC'CwG_GG
Cuts at: 7566 7566
Size: 11144
PciI A'CATG_T
Cuts at: 115 788 1250 2484 9600 115
Size: 673 462 1234 7116 1659
Fragments arranged by size:
7116 1659 1234 673 462
PfoI T'CCnGG_A
Cuts at: 7803 7803
Size: 11144
PmeI GTTT'AAAC
Cuts at: 8143 8143
Size: 11144
PmlI CAC'GTG
Cuts at: 7958 7958
Size: 11144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10283 10315 10283
Size: 32 11112
PpuMI rG'GwC_Cy
Cuts at: 3982 7385 7683 7719 7854 3982
Size: 3403 298 36 135 7272
Fragments arranged by size:
7272 3403 298 135 36
PshAI GACnn'nnGTC
Cuts at: 2945 2945
Size: 11144
PsiI TTA'TAA
Cuts at: 1964 4295 4658 6926 1964
Size: 2331 363 2268 6182
Fragments arranged by size:
6182 2331 2268 363
PspOMI G'GGCC_C
Cuts at: 5266 5266
Size: 11144
PspXI vC'TCGA_Gb
Cuts at: 7788 7788
Size: 11144
PstI C_TGCA'G
Cuts at: 6811 7517 8191 8876 6811
Size: 706 674 685 9079
Fragments arranged by size:
9079 706 685 674
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11144
PvuII CAG'CTG
Cuts at: 1719 3066 3958 6142 6378 6635 7416 7936 8569
Size: 1347 892 2184 236 257 781 520 633
Cuts at: 8569 8929 1719
Size: 360 3934
Fragments arranged by size:
3934 2184 1347 892 781 633 520 360
257 236
RsrII CG'GwC_CG
Cuts at: 9339 9339
Size: 11144
SacI G_AGCT'C
Cuts at: 6405 7994 8158 6405
Size: 1589 164 9391
Fragments arranged by size:
9391 1589 164
SalI G'TCGA_C
Cuts at: 8181 8181
Size: 11144
SapI GCTCTTCn'nnn_
Cuts at: 9167 9377 9167
Size: 210 10934
SbfI CC_TGCA'GG
Cuts at: 8191 8191
Size: 11144
ScaI AGT'ACT
Cuts at: 2514 4917 10661 2514
Size: 2403 5744 2997
Fragments arranged by size:
5744 2997 2403
SfcI C'TryA_G
Cuts at: 33 2096 3446 3908 4439 6807 6862 7470 7513
Size: 2063 1350 462 531 2368 55 608 43
Cuts at: 7513 7883 8187 8872 9865 10056 33
Size: 370 304 685 993 191 1121
Fragments arranged by size:
2368 2063 1350 1121 993 685 608 531
462 370 304 191 55 43
SfoI GGC'GCC
Cuts at: 8576 8824 8576
Size: 248 10896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11144
SmaI CCC'GGG
Cuts at: 8166 8166
Size: 11144
SmlI C'TyrA_G
Cuts at: 850 1369 3014 3196 3229 5295 5696 5898 6595
Size: 519 1645 182 33 2066 401 202 697
Cuts at: 6595 7501 7788 8299 9214 9706 9968 850
Size: 906 287 511 915 492 262 2026
Fragments arranged by size:
2066 2026 1645 915 906 697 519 511
492 401 287 262 202 182 33
SpeI A'CTAG_T
Cuts at: 67 1837 4138 67
Size: 1770 2301 7073
Fragments arranged by size:
7073 2301 1770
SphI G_CATG'C
Cuts at: 4359 8197 8697 4359
Size: 3838 500 6806
Fragments arranged by size:
6806 3838 500
SspI AAT'ATT
Cuts at: 5149 7766 5149
Size: 2617 8527
StuI AGG'CCT
Cuts at: 3633 3633
Size: 11144
StyI C'CwwG_G
Cuts at: 863 1005 7293 7652 8278 8632 863
Size: 142 6288 359 626 354 3375
Fragments arranged by size:
6288 3375 626 359 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1369 1551 4492 8727 8860 9319 9528 1369
Size: 182 2941 4235 133 459 209 2985
Fragments arranged by size:
4235 2985 2941 459 209 182 133
TatI w'GTAC_w
Cuts at: 1281 1415 1698 2230 2284 2512 3475 3787 3793
Size: 134 283 532 54 228 963 312 6
Cuts at: 3793 4216 4300 4757 4915 5851 6878 7355 10659
Size: 423 84 457 158 936 1027 477 3304
Cuts at: 10659 1281
Size: 1766
Fragments arranged by size:
3304 1766 1027 963 936 532 477 457
423 312 283 228 158 134 84 54
6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7518 7550 10661 10693 7518
Size: 32 3111 32 7969
Fragments arranged by size:
7969 3111 32 32
Tth111I GACn'n_nGTC
Cuts at: 1465 6245 8941 1465
Size: 4780 2696 3668
Fragments arranged by size:
4780 3668 2696
XbaI T'CTAG_A
Cuts at: 1999 6366 8175 1999
Size: 4367 1809 4968
Fragments arranged by size:
4968 4367 1809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 3118 5790 7300 8639 2158
Size: 960 2672 1510 1339 4663
Fragments arranged by size:
4663 2672 1510 1339 960
XhoI C'TCGA_G
Cuts at: 7788 7788
Size: 11144
XmaI C'CCGG_G
Cuts at: 8164 8164
Size: 11144
XmnI GAAnn'nnTTC
Cuts at: 605 1478 3720 4200 4673 6962 605
Size: 873 2242 480 473 2289 4787
Fragments arranged by size:
4787 2289 2242 873 480 473
ZraI GAC'GTC
Cuts at: 1997 1997
Size: 11144
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AscI AvrII BbvCI BglI BmtI BsiWI
BssHII BstEII Bsu36I CspCI FseI FspAI NheI NotI
NruI PacI PflMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI