Restriction map for
pF1KA0627
(Circular) (Six-base) MAPSORT of: pF1KA0627.seq Check: 2530 from: 1 to: 6995
pF1KA0627 6995 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 241 1231 1288 241
Size: 990 57 5948
Fragments arranged by size:
5948 990 57
AccI GT'mk_AC
Cuts at: 1126 1848 4033 1126
Size: 722 2185 4088
Fragments arranged by size:
4088 2185 722
Acc65I G'GTAC_C
Cuts at: 4011 4011
Size: 6995
AclI AA'CG_TT
Cuts at: 6835 6835
Size: 6995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 653 1388 2407 2930 2963 4820 5252 5999
Size: 187 735 1019 523 33 1857 432 747
Cuts at: 5999 6267 466
Size: 268 1194
Fragments arranged by size:
1857 1194 1019 747 735 523 432 268
187 33
AflIII A'CryG_T
Cuts at: 4347 5451 6428 4347
Size: 1104 977 4914
Fragments arranged by size:
4914 1104 977
AgeI A'CCGG_T
Cuts at: 5383 5383
Size: 6995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3667 3667
Size: 6995
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 228
Size: 32 6963
AlwNI CAG_nnn'CTG
Cuts at: 1366 1894 3949 4300 5867 1366
Size: 528 2055 351 1567 2494
Fragments arranged by size:
2494 2055 1567 528 351
ApaLI G'TGCA_C
Cuts at: 3935 5765 3935
Size: 1830 5165
ApoI r'AATT_y
Cuts at: 398 452 808 893 2198 2547 2955 3999 6348
Size: 54 356 85 1305 349 408 1044 2349
Cuts at: 6348 6604 398
Size: 256 789
Fragments arranged by size:
2349 1305 1044 789 408 356 349 256
85 54
AseI AT'TA_AT
Cuts at: 20 3689 20
Size: 3669 3326
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6995
AvaI C'yCGr_G
Cuts at: 2576 3155 4015 2576
Size: 579 860 5556
Fragments arranged by size:
5556 860 579
AvrII C'CTAG_G
Cuts at: 2130 2130
Size: 6995
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 719 752 719
Size: 33 6962
BamHI G'GATC_C
Cuts at: 2529 4020 2529
Size: 1491 5504
BanI G'GyrC_C
Cuts at: 1243 1300 1783 4011 4425 4673 4708 1243
Size: 57 483 2228 414 248 35 3530
Fragments arranged by size:
3530 2228 483 414 248 57 35
BanII G_rGCy'C
Cuts at: 1540 4009 5039 1540
Size: 2469 1030 3496
Fragments arranged by size:
3496 2469 1030
BbeI G_GCGC'C
Cuts at: 4429 4677 4429
Size: 248 6747
BbsI GAAGACnn'nnnn_
Cuts at: 1009 1850 3822 1009
Size: 841 1972 4182
Fragments arranged by size:
4182 1972 841
BbvCI CC'TCA_GC
Cuts at: 911 1572 3596 911
Size: 661 2024 4310
Fragments arranged by size:
4310 2024 661
BciVI GTATCCnnnnn_n'
Cuts at: 1329 4893 5654 6995 1329
Size: 3564 761 1341 1329
Fragments arranged by size:
3564 1341 1329 761
BclI T'GATC_A
Cuts at: 621 2454 4515 621
Size: 1833 2061 3101
Fragments arranged by size:
3101 2061 1833
BfrBI ATG'CAT
Cuts at: 1965 1993 1965
Size: 28 6967
BglII A'GATC_T
Cuts at: 3589 4510 6547 3589
Size: 921 2037 4037
Fragments arranged by size:
4037 2037 921
BlpI GC'TnA_GC
Cuts at: 4107 4107
Size: 6995
Bme1580I G_kGCm'C
Cuts at: 1305 1788 3939 4620 4713 5769 1305
Size: 483 2151 681 93 1056 2531
Fragments arranged by size:
2531 2151 1056 681 483 93
BmrI ACTGGGnnnn_n'
Cuts at: 432 4207 4288 4623 6767 432
Size: 3775 81 335 2144 660
Fragments arranged by size:
3775 2144 660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5362 5362
Size: 6995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 494 1481 1743 3971 314
Size: 180 987 262 2228 3338
Fragments arranged by size:
3338 2228 987 262 180
Bpu10I CC'TnA_GC
Cuts at: 542 911 1290 1572 3596 542
Size: 369 379 282 2024 3941
Fragments arranged by size:
3941 2024 379 369 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 3069 3199 4171 5050 5542 5840 1015
Size: 2054 130 972 879 492 298 2170
Fragments arranged by size:
2170 2054 972 879 492 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 1499 6699 1499
Size: 5200 1795
BsaAI yAC'GTr
Cuts at: 4348 4348
Size: 6995
BsaBI GATnn'nnATC
Cuts at: 6 1950 4509 6546 6
Size: 1944 2559 2037 455
Fragments arranged by size:
2559 2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 1979 4426 4674 5376 6872 1979
Size: 2447 248 702 1496 2102
Fragments arranged by size:
2447 2102 1496 702 248
BsaWI w'CCGG_w
Cuts at: 211 4184 4705 5383 5657 5804 6215 211
Size: 3973 521 678 274 147 411 991
Fragments arranged by size:
3973 991 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2853 2883 2853
Size: 30 6965
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 807 1918 3132 3968 807
Size: 1111 1214 836 3834
Fragments arranged by size:
3834 1214 1111 836
BseYI C'CCAG_C
Cuts at: 4420 5178 5755 6251 4420
Size: 758 577 496 5164
Fragments arranged by size:
5164 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 464 722 1316 182
Size: 282 258 594 5861
Fragments arranged by size:
5861 594 282 258
BsiEI CG_ry'CG
Cuts at: 104 4537 4583 5791 104
Size: 4433 46 1208 1308
Fragments arranged by size:
4433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 528 1540 3939 4009 4787 4977 5769 528
Size: 1012 2399 70 778 190 792 1754
Fragments arranged by size:
2399 1754 1012 792 778 190 70
BsmI GAATG_Cn'
Cuts at: 1236 1314 1965 1967 1236
Size: 78 651 2 6264
Fragments arranged by size:
6264 651 78 2
BsmBI CGTCTCn'nnnn_
Cuts at: 789 789
Size: 6995
Bsp1286I G_dGCh'C
Cuts at: 528 1305 1540 1788 3939 4009 4620 4713 4787
Size: 777 235 248 2151 70 611 93 74
Cuts at: 4787 4977 5039 5769 528
Size: 190 62 730 1754
Fragments arranged by size:
2151 1754 777 730 611 248 235 190
93 74 70 62
BspEI T'CCGG_A
Cuts at: 211 6215 211
Size: 6004 991
BspHI T'CATG_A
Cuts at: 897 3542 6171 897
Size: 2645 2629 1721
Fragments arranged by size:
2645 2629 1721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 241 1231 1288 1303 3761 4045 4259 4561 4942
Size: 990 57 15 2458 284 214 302 381
Cuts at: 4942 241
Size: 2294
Fragments arranged by size:
2458 2294 990 381 302 284 214 57
15
BsrBI CCG'CTC
Cuts at: 50 5287 5341 6825 6990 50
Size: 5237 54 1484 165 55
Fragments arranged by size:
5237 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2763 3115 4907 2763
Size: 352 1792 4851
Fragments arranged by size:
4851 1792 352
BsrFI r'CCGG_y
Cuts at: 1484 4993 5174 5383 1484
Size: 3509 181 209 3096
Fragments arranged by size:
3509 3096 209 181
BssSI C'ACGA_G
Cuts at: 5266 5624 5266
Size: 358 6637
BstAPI GCAn_nnn'nTGC
Cuts at: 1258 1405 1999 4372 1258
Size: 147 594 2373 3881
Fragments arranged by size:
3881 2373 594 147
BstBI TT'CG_AA
Cuts at: 2745 5356 2745
Size: 2611 4384
BstXI CCAn_nnnn'nTGG
Cuts at: 114 964 2526 2819 114
Size: 850 1562 293 4290
Fragments arranged by size:
4290 1562 850 293
BstYI r'GATC_y
Cuts at: 1 546 553 2359 2529 3567 3589 4020 4487
Size: 545 7 1806 170 1038 22 431 467
Cuts at: 4487 4510 4845 5091 6103 6189 6201 6547 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1806 1038 1012 545 467 449 431 346
335 246 170 86 23 22 12 7
Bsu36I CC'TnA_GG
Cuts at: 260 1815 3496 260
Size: 1555 1681 3759
Fragments arranged by size:
3759 1681 1555
BtgI C'CryG_G
Cuts at: 107 1597 2774 107
Size: 1490 1177 4328
Fragments arranged by size:
4328 1490 1177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2003 4945 5126 5289 2003
Size: 2942 181 163 3709
Fragments arranged by size:
3709 2942 181 163
BtsI GCAGTG_nn'
Cuts at: 460 1050 2576 3646 3816 4238 460
Size: 590 1526 1070 170 422 3217
Fragments arranged by size:
3217 1526 1070 590 422 170
DraI TTT'AAA
Cuts at: 3519 3994 3519
Size: 475 6520
DraIII CAC_nnn'GTG
Cuts at: 1216 1216
Size: 6995
DrdI GACnn_nn'nnGTC
Cuts at: 4701 5559 4701
Size: 858 6137
EaeI y'GGCC_r
Cuts at: 1374 2516 3617 4580 4754 5145 5172 6517 1374
Size: 1142 1101 963 174 391 27 1345 1852
Fragments arranged by size:
1852 1345 1142 1101 963 391 174 27
EagI C'GGCC_G
Cuts at: 4580 4580
Size: 6995
EarI CTCTTCn'nnn_
Cuts at: 1006 1061 1149 1497 5018 5228 1006
Size: 55 88 348 3521 210 2773
Fragments arranged by size:
3521 2773 348 210 88 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5203 5513 5659 6805 5203
Size: 310 146 1146 5393
Fragments arranged by size:
5393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1538 4007 1538
Size: 2469 4526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 466 494 653 1388 1481 1743 2407 2930
Size: 152 28 159 735 93 262 664 523
Cuts at: 2930 2963 3971 4820 5252 5999 6267 314
Size: 33 1008 849 432 747 268 1042
Fragments arranged by size:
1042 1008 849 747 735 664 523 432
268 262 159 152 93 33 28
EcoO109I rG'GnC_Cy
Cuts at: 1073 1811 4134 1073
Size: 738 2323 3934
Fragments arranged by size:
3934 2323 738
EcoRI G'AATT_C
Cuts at: 893 3999 893
Size: 3106 3889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2423 5014 2311
Size: 112 2591 4292
Fragments arranged by size:
4292 2591 112
FspI TGC'GCA
Cuts at: 4776 4776
Size: 6995
HaeII r_GCGC'y
Cuts at: 632 1290 4429 4677 5699 6678 632
Size: 658 3139 248 1022 979 949
Fragments arranged by size:
3139 1022 979 949 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 538 570 1060 1092 1544 1576 3718
Size: 32 376 32 490 32 452 32 2142
Cuts at: 3718 3750 4837 4867 4869 4899 130
Size: 32 1087 30 2 30 2226
Fragments arranged by size:
2226 2142 1087 490 452 376 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1849 3889 4034 1849
Size: 2040 145 4810
Fragments arranged by size:
4810 2040 145
HindIII A'AGCT_T
Cuts at: 129 323 572 2472 2725 3110 3821 129
Size: 194 249 1900 253 385 711 3303
Fragments arranged by size:
3303 1900 711 385 253 249 194
KasI G'GCGC_C
Cuts at: 4425 4673 4425
Size: 248 6747
KpnI G_GTAC'C
Cuts at: 4015 4015
Size: 6995
MfeI C'AATT_G
Cuts at: 765 2103 765
Size: 1338 5657
MluI A'CGCG_T
Cuts at: 6428 6428
Size: 6995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 246 497 5666 5850 246
Size: 251 5169 184 1391
Fragments arranged by size:
5169 1391 251 184
MscI TGG'CCA
Cuts at: 2518 3619 4756 2518
Size: 1101 1137 4757
Fragments arranged by size:
4757 1137 1101
MslI CAynn'nnrTG
Cuts at: 112 731 1225 1970 2209 2288 3649 4984 5111
Size: 619 494 745 239 79 1361 1335 127
Cuts at: 5111 112
Size: 1996
Fragments arranged by size:
1996 1361 1335 745 619 494 239 127
79
MspA1I CmG'CkG
Cuts at: 175 4106 4420 4780 5793 6038 175
Size: 3931 314 360 1013 245 1132
Fragments arranged by size:
3931 1132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5176 5176
Size: 6995
NarI GG'CG_CC
Cuts at: 4426 4674 4426
Size: 248 6747
NcoI C'CATG_G
Cuts at: 107 2774 107
Size: 2667 4328
NdeI CA'TA_TG
Cuts at: 1956 1956
Size: 6995
NgoMIV G'CCGG_C
Cuts at: 5174 5174
Size: 6995
NsiI A_TGCA'T
Cuts at: 1967 1995 1967
Size: 28 6967
NspI r_CATG'y
Cuts at: 862 2250 4048 4548 5455 862
Size: 1388 1798 500 907 2402
Fragments arranged by size:
2402 1798 1388 907 500
PciI A'CATG_T
Cuts at: 5451 5451
Size: 6995
PflMI CCAn_nnn'nTGG
Cuts at: 1408 1828 1408
Size: 420 6575
PfoI T'CCnGG_A
Cuts at: 1191 1497 2532 1191
Size: 306 1035 5654
Fragments arranged by size:
5654 1035 306
PmeI GTTT'AAAC
Cuts at: 3994 3994
Size: 6995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 783 815 6134 6166 783
Size: 32 5319 32 1612
Fragments arranged by size:
5319 1612 32 32
PpuMI rG'GwC_Cy
Cuts at: 1073 1073
Size: 6995
PshAI GACnn'nnGTC
Cuts at: 1727 1854 3086 1727
Size: 127 1232 5636
Fragments arranged by size:
5636 1232 127
PsiI TTA'TAA
Cuts at: 319 2545 319
Size: 2226 4769
PstI C_TGCA'G
Cuts at: 1205 1242 2141 3678 4042 4727 1205
Size: 37 899 1537 364 685 3473
Fragments arranged by size:
3473 1537 899 685 364 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6995
PvuII CAG'CTG
Cuts at: 175 4420 4780 175
Size: 4245 360 2390
Fragments arranged by size:
4245 2390 360
RsrII CG'GwC_CG
Cuts at: 5190 5190
Size: 6995
SacI G_AGCT'C
Cuts at: 1540 4009 1540
Size: 2469 4526
SalI G'TCGA_C
Cuts at: 1847 4032 1847
Size: 2185 4810
SapI GCTCTTCn'nnn_
Cuts at: 5018 5228 5018
Size: 210 6785
SbfI CC_TGCA'GG
Cuts at: 4042 4042
Size: 6995
ScaI AGT'ACT
Cuts at: 6512 6512
Size: 6995
SfcI C'TryA_G
Cuts at: 33 584 1127 1201 1238 1611 2019 2137 3674
Size: 551 543 74 37 373 408 118 1537
Cuts at: 3674 4038 4723 5716 5907 33
Size: 364 685 993 191 1121
Fragments arranged by size:
1537 1121 993 685 551 543 408 373
364 191 118 74 37
SfoI GGC'GCC
Cuts at: 4427 4675 4427
Size: 248 6747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6995
SmaI CCC'GGG
Cuts at: 4017 4017
Size: 6995
SmlI C'TyrA_G
Cuts at: 994 3084 3155 3214 4150 5065 5557 5819 994
Size: 2090 71 59 936 915 492 262 2170
Fragments arranged by size:
2170 2090 936 915 492 262 71 59
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6995
SphI G_CATG'C
Cuts at: 4048 4548 4048
Size: 500 6495
SspI AAT'ATT
Cuts at: 2433 3416 2433
Size: 983 6012
StuI AGG'CCT
Cuts at: 2126 2126
Size: 6995
StyI C'CwwG_G
Cuts at: 107 1247 1424 2130 2774 3737 4129 4483 107
Size: 1140 177 706 644 963 392 354 2619
Fragments arranged by size:
2619 1140 963 706 644 392 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2332 4578 4711 5170 5379 2332
Size: 2246 133 459 209 3948
Fragments arranged by size:
3948 2246 459 209 133
TatI w'GTAC_w
Cuts at: 2313 6510 2313
Size: 4197 2798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1259 1291 3810 3842 6512 6544 1259
Size: 32 2519 32 2670 32 1710
Fragments arranged by size:
2670 2519 1710 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4792 4792
Size: 6995
XbaI T'CTAG_A
Cuts at: 1435 4026 1435
Size: 2591 4404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4490 4490
Size: 6995
XhoI C'TCGA_G
Cuts at: 3155 3155
Size: 6995
XmaI C'CCGG_G
Cuts at: 4015 4015
Size: 6995
XmnI GAAnn'nnTTC
Cuts at: 1965 1965
Size: 6995
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI BcgI
BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI NheI
NotI NruI PacI PasI PmlI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
ZraI