Restriction map for
pF1KA0633
(Circular) (Six-base) MAPSORT of: pF1KA0633.seq Check: 3085 from: 1 to: 6896
pF1KA0633 6896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1245 3604 1245
Size: 2359 4537
AatII G_ACGT'C
Cuts at: 302 1928 302
Size: 1626 5270
AccI GT'mk_AC
Cuts at: 2992 3934 2992
Size: 942 5954
Acc65I G'GTAC_C
Cuts at: 3912 3912
Size: 6896
AclI AA'CG_TT
Cuts at: 6736 6736
Size: 6896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 419 545 2399 4721 5153 5900 6168 373
Size: 46 126 1854 2322 432 747 268 1101
Fragments arranged by size:
2322 1854 1101 747 432 268 126 46
AflII C'TTAA_G
Cuts at: 2209 2209
Size: 6896
AflIII A'CryG_T
Cuts at: 773 4248 5352 6329 773
Size: 3475 1104 977 1340
Fragments arranged by size:
3475 1340 1104 977
AgeI A'CCGG_T
Cuts at: 2115 5284 2115
Size: 3169 3727
AleI CACnn'nnGTG
Cuts at: 2491 2491
Size: 6896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2582 2582
Size: 6896
AlwNI CAG_nnn'CTG
Cuts at: 1232 1260 3136 3859 4201 5768 1232
Size: 28 1876 723 342 1567 2360
Fragments arranged by size:
2360 1876 1567 723 342 28
ApaI G_GGCC'C
Cuts at: 240 240
Size: 6896
ApaLI G'TGCA_C
Cuts at: 205 5666 205
Size: 5461 1435
ApoI r'AATT_y
Cuts at: 425 847 2106 3388 3900 6249 6505 425
Size: 422 1259 1282 512 2349 256 816
Fragments arranged by size:
2349 1282 1259 816 512 422 256
AseI AT'TA_AT
Cuts at: 20 1558 20
Size: 1538 5358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
AvaI C'yCGr_G
Cuts at: 241 754 3916 241
Size: 513 3162 3221
Fragments arranged by size:
3221 3162 513
AvrII C'CTAG_G
Cuts at: 2592 2956 2592
Size: 364 6532
BamHI G'GATC_C
Cuts at: 3921 3921
Size: 6896
BanI G'GyrC_C
Cuts at: 555 1845 1989 2157 2171 2643 2805 2890 3592
Size: 1290 144 168 14 472 162 85 702
Cuts at: 3592 3793 3876 3912 4326 4574 4609 555
Size: 201 83 36 414 248 35 2842
Fragments arranged by size:
2842 1290 702 472 414 248 201 168
162 144 85 83 36 35 14
BanII G_rGCy'C
Cuts at: 240 625 755 770 1014 1251 1474 2467 2742
Size: 385 130 15 244 237 223 993 275
Cuts at: 2742 2952 3143 3638 3676 3910 4940 240
Size: 210 191 495 38 234 1030 2196
Fragments arranged by size:
2196 1030 993 495 385 275 244 237
234 223 210 191 130 38 15
BbeI G_GCGC'C
Cuts at: 1849 1993 2809 4330 4578 1849
Size: 144 816 1521 248 4167
Fragments arranged by size:
4167 1521 816 248 144
BbsI GAAGACnn'nnnn_
Cuts at: 425 1424 1556 1607 1625 2357 3041 3697 3701
Size: 999 132 51 18 732 684 656 4
Cuts at: 3701 425
Size: 3620
Fragments arranged by size:
3620 999 732 684 656 132 51 18
4
BbvCI CC'TCA_GC
Cuts at: 280 3799 280
Size: 3519 3377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1699 1733 3541 3575 1699
Size: 34 1808 34 5020
Fragments arranged by size:
5020 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4794 5555 6896 4794
Size: 761 1341 4794
Fragments arranged by size:
4794 1341 761
BclI T'GATC_A
Cuts at: 4416 4416
Size: 6896
BglI GCCn_nnn'nGGC
Cuts at: 130 321 130
Size: 191 6705
BglII A'GATC_T
Cuts at: 4411 6448 4411
Size: 2037 4859
BlpI GC'TnA_GC
Cuts at: 1859 3129 4008 1859
Size: 1270 879 4747
Fragments arranged by size:
4747 1270 879
Bme1580I G_kGCm'C
Cuts at: 209 240 2498 2895 3587 3796 3881 4521 4614
Size: 31 2258 397 692 209 85 640 93
Cuts at: 4614 5670 209
Size: 1056 1435
Fragments arranged by size:
2258 1435 1056 692 640 397 209 93
85 31
BmrI ACTGGGnnnn_n'
Cuts at: 646 1720 2889 3311 4108 4189 4524 6668 646
Size: 1074 1169 422 797 81 335 2144 874
Fragments arranged by size:
2144 1169 1074 874 797 422 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3144 5263 3144
Size: 2119 4777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 622 748 1007 1586 2439 3248 3622 3762
Size: 276 126 259 579 853 809 374 140
Cuts at: 3762 346
Size: 3480
Fragments arranged by size:
3480 853 809 579 374 276 259 140
126
Bpu10I CC'TnA_GC
Cuts at: 280 3668 3799 280
Size: 3388 131 3377
Fragments arranged by size:
3388 3377 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2662 4072 4951 5443 5741 2072
Size: 590 1410 879 492 298 3227
Fragments arranged by size:
3227 1410 879 590 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1270 3402 6600 1270
Size: 2132 3198 1566
Fragments arranged by size:
3198 2132 1566
BsaAI yAC'GTr
Cuts at: 690 2637 4249 690
Size: 1947 1612 3337
Fragments arranged by size:
3337 1947 1612
BsaBI GATnn'nnATC
Cuts at: 6 1973 4410 6447 6
Size: 1967 2437 2037 455
Fragments arranged by size:
2437 2037 1967 455
BsaHI Gr'CG_yC
Cuts at: 299 1846 1925 1990 2806 3836 4327 4575 5277
Size: 1547 79 65 816 1030 491 248 702
Cuts at: 5277 6773 299
Size: 1496 422
Fragments arranged by size:
1547 1496 1030 816 702 491 422 248
79 65
BsaWI w'CCGG_w
Cuts at: 2115 2290 4085 4606 5284 5558 5705 6116 2115
Size: 175 1795 521 678 274 147 411 2895
Fragments arranged by size:
2895 1795 678 521 411 274 175 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1267 1297 1627 1657 3010 3040 1267
Size: 30 330 30 1353 30 5123
Fragments arranged by size:
5123 1353 330 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 348 688 736 1007 1010 1195 1288 2903 3646
Size: 340 48 271 3 185 93 1615 743
Cuts at: 3646 348
Size: 3598
Fragments arranged by size:
3598 1615 743 340 271 185 93 48
3
BseYI C'CCAG_C
Cuts at: 942 960 1798 2313 2555 3364 4321 5079 5656
Size: 18 838 515 242 809 957 758 577
Cuts at: 5656 6152 942
Size: 496 1686
Fragments arranged by size:
1686 957 838 809 758 577 515 496
242 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1891 2804 3418 3545 3746 1891
Size: 913 614 127 201 5041
Fragments arranged by size:
5041 913 614 201 127
BsiEI CG_ry'CG
Cuts at: 104 146 1319 4438 4484 5692 104
Size: 42 1173 3119 46 1208 1308
Fragments arranged by size:
3119 1308 1208 1173 46 42
BsiHKAI G_wGCw'C
Cuts at: 171 209 344 689 755 770 1198 1865 2467
Size: 38 135 345 66 15 428 667 602
Cuts at: 2467 3638 3910 4688 4878 5670 171
Size: 1171 272 778 190 792 1397
Fragments arranged by size:
1397 1171 792 778 667 602 428 345
272 190 135 66 38 15
BsmBI CGTCTCn'nnnn_
Cuts at: 1362 1362
Size: 6896
Bsp1286I G_dGCh'C
Cuts at: 171 209 240 344 625 689 755 770 1014
Size: 38 31 104 281 64 66 15 244
Cuts at: 1014 1198 1251 1474 1865 2467 2498 2742 2895
Size: 184 53 223 391 602 31 244 153
Cuts at: 2895 2952 3143 3587 3638 3676 3796 3881 3910
Size: 57 191 444 51 38 120 85 29
Cuts at: 3910 4521 4614 4688 4878 4940 5670 171
Size: 611 93 74 190 62 730 1397
Fragments arranged by size:
1397 730 611 602 444 391 281 244
244 223 191 190 184 153 120 104
93 85 74 66 64 62 57 53
51 38 38 31 31 29 15
BspEI T'CCGG_A
Cuts at: 6116 6116
Size: 6896
BspHI T'CATG_A
Cuts at: 6072 6072
Size: 6896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 1245 1908 3015 3604 3946 4160 4462 4843
Size: 646 663 1107 589 342 214 302 381
Cuts at: 4843 599
Size: 2652
Fragments arranged by size:
2652 1107 663 646 589 381 342 302
214
BsrBI CCG'CTC
Cuts at: 50 3845 5188 5242 6726 6891 50
Size: 3795 1343 54 1484 165 55
Fragments arranged by size:
3795 1484 1343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 710 825 2720 4808 710
Size: 115 1895 2088 2798
Fragments arranged by size:
2798 2088 1895 115
BsrFI r'CCGG_y
Cuts at: 2115 2341 4894 5075 5284 2115
Size: 226 2553 181 209 3727
Fragments arranged by size:
3727 2553 226 209 181
BssHII G'CGCG_C
Cuts at: 120 3231 120
Size: 3111 3785
BssSI C'ACGA_G
Cuts at: 164 3886 5167 5525 164
Size: 3722 1281 358 1535
Fragments arranged by size:
3722 1535 1281 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1232 3622 3664 4273 1232
Size: 2390 42 609 3855
Fragments arranged by size:
3855 2390 609 42
BstBI TT'CG_AA
Cuts at: 2014 5257 2014
Size: 3243 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 294 2168 2628 294
Size: 1874 460 4562
Fragments arranged by size:
4562 1874 460
BstYI r'GATC_y
Cuts at: 1 3921 4388 4411 4746 4992 6004 6090 6102
Size: 3920 467 23 335 246 1012 86 12
Cuts at: 6102 6448 1
Size: 346 449
Fragments arranged by size:
3920 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 551 626 700 3604 551
Size: 75 74 2904 3843
Fragments arranged by size:
3843 2904 75 74
BtgI C'CryG_G
Cuts at: 107 293 305 1662 2621 2925 3243 3579 107
Size: 186 12 1357 959 304 318 336 3424
Fragments arranged by size:
3424 1357 959 336 318 304 186 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 373 2274 4846 5027 5190 373
Size: 1901 2572 181 163 2079
Fragments arranged by size:
2572 2079 1901 181 163
BtsI GCAGTG_nn'
Cuts at: 1230 1431 1677 2948 3562 4139 1230
Size: 201 246 1271 614 577 3987
Fragments arranged by size:
3987 1271 614 577 246 201
DraI TTT'AAA
Cuts at: 3895 3895
Size: 6896
DrdI GACnn_nn'nnGTC
Cuts at: 4602 5460 4602
Size: 858 6038
EaeI y'GGCC_r
Cuts at: 131 3358 4481 4655 5046 5073 6418 131
Size: 3227 1123 174 391 27 1345 609
Fragments arranged by size:
3227 1345 1123 609 391 174 27
EagI C'GGCC_G
Cuts at: 4481 4481
Size: 6896
EarI CTCTTCn'nnn_
Cuts at: 325 521 783 1186 1462 4919 5129 325
Size: 196 262 403 276 3457 210 2092
Fragments arranged by size:
3457 2092 403 276 262 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1285 5104 5414 5560 6706 1285
Size: 3819 310 146 1146 1475
Fragments arranged by size:
3819 1475 1146 310 146
EcoICRI GAG'CTC
Cuts at: 753 768 2465 3636 3908 753
Size: 15 1697 1171 272 3741
Fragments arranged by size:
3741 1697 1171 272 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 373 419 545 622 748 1007 1586 2399
Size: 27 46 126 77 126 259 579 813
Cuts at: 2399 2439 3248 3622 3762 4721 5153 5900 6168
Size: 40 809 374 140 959 432 747 268
Cuts at: 6168 346
Size: 1074
Fragments arranged by size:
1074 959 813 809 747 579 432 374
268 259 140 126 126 77 46 40
27
EcoNI CCTnn'n_nnAGG
Cuts at: 1022 2596 3204 3266 3607 1022
Size: 1574 608 62 341 4311
Fragments arranged by size:
4311 1574 608 341 62
EcoO109I rG'GnC_Cy
Cuts at: 236 237 482 1434 1459 2476 4035 236
Size: 1 245 952 25 1017 1559 3097
Fragments arranged by size:
3097 1559 1017 952 245 25 1
EcoRI G'AATT_C
Cuts at: 2106 3900 2106
Size: 1794 5102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1491 3897 4915 1491
Size: 2406 1018 3472
Fragments arranged by size:
3472 2406 1018
FspI TGC'GCA
Cuts at: 4677 4677
Size: 6896
HaeII r_GCGC'y
Cuts at: 280 1008 1114 1849 1993 2809 4330 4578 5600
Size: 728 106 735 144 816 1521 248 1022
Cuts at: 5600 6579 280
Size: 979 597
Fragments arranged by size:
1521 1022 979 816 735 728 597 248
144 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 1039 1071 1267 1299 1342 1374 1627
Size: 32 860 32 196 32 43 32 253
Cuts at: 1627 1659 2496 2528 2836 2868 4738 4768 4770
Size: 32 837 32 308 32 1870 30 2
Cuts at: 4770 4800 147
Size: 30 2243
Fragments arranged by size:
2243 1870 860 837 308 253 196 43
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3935 3935
Size: 6896
KasI G'GCGC_C
Cuts at: 1845 1989 2805 4326 4574 1845
Size: 144 816 1521 248 4167
Fragments arranged by size:
4167 1521 816 248 144
KpnI G_GTAC'C
Cuts at: 3916 3916
Size: 6896
MluI A'CGCG_T
Cuts at: 773 6329 773
Size: 5556 1340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 1286 1697 1908 5567 5751 971
Size: 315 411 211 3659 184 2116
Fragments arranged by size:
3659 2116 411 315 211 184
MscI TGG'CCA
Cuts at: 3360 4657 3360
Size: 1297 5599
MslI CAynn'nnrTG
Cuts at: 2491 2626 2756 2777 2877 4885 5012 2491
Size: 135 130 21 100 2008 127 4375
Fragments arranged by size:
4375 2008 135 130 127 100 21
MspA1I CmG'CkG
Cuts at: 1295 3581 3590 4007 4321 4681 5694 5939 1295
Size: 2286 9 417 314 360 1013 245 2252
Fragments arranged by size:
2286 2252 1013 417 360 314 245 9
NaeI GCC'GGC
Cuts at: 5077 5077
Size: 6896
NarI GG'CG_CC
Cuts at: 1846 1990 2806 4327 4575 1846
Size: 144 816 1521 248 4167
Fragments arranged by size:
4167 1521 816 248 144
NcoI C'CATG_G
Cuts at: 107 293 1662 2621 107
Size: 186 1369 959 4382
Fragments arranged by size:
4382 1369 959 186
NgoMIV G'CCGG_C
Cuts at: 5075 5075
Size: 6896
NspI r_CATG'y
Cuts at: 205 3949 4449 5356 205
Size: 3744 500 907 1745
Fragments arranged by size:
3744 1745 907 500
PasI CC'CwG_GG
Cuts at: 1215 1994 2191 1215
Size: 779 197 5920
Fragments arranged by size:
5920 779 197
PciI A'CATG_T
Cuts at: 5352 5352
Size: 6896
PflMI CCAn_nnn'nTGG
Cuts at: 181 2709 181
Size: 2528 4368
PfoI T'CCnGG_A
Cuts at: 180 3264 180
Size: 3084 3812
PmeI GTTT'AAAC
Cuts at: 3895 3895
Size: 6896
PmlI CAC'GTG
Cuts at: 690 2637 690
Size: 1947 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6035 6067 6035
Size: 32 6864
PpuMI rG'GwC_Cy
Cuts at: 482 1434 1459 2476 482
Size: 952 25 1017 4902
Fragments arranged by size:
4902 1017 952 25
PshAI GACnn'nnGTC
Cuts at: 1457 3701 1457
Size: 2244 4652
PspOMI G'GGCC_C
Cuts at: 236 236
Size: 6896
PstI C_TGCA'G
Cuts at: 1237 1426 3205 3601 3943 4628 1237
Size: 189 1779 396 342 685 3505
Fragments arranged by size:
3505 1779 685 396 342 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6896
PvuII CAG'CTG
Cuts at: 1295 4321 4681 1295
Size: 3026 360 3510
Fragments arranged by size:
3510 3026 360
RsrII CG'GwC_CG
Cuts at: 5091 5091
Size: 6896
SacI G_AGCT'C
Cuts at: 755 770 2467 3638 3910 755
Size: 15 1697 1171 272 3741
Fragments arranged by size:
3741 1697 1171 272 15
SacII CC_GC'GG
Cuts at: 3582 3582
Size: 6896
SalI G'TCGA_C
Cuts at: 3933 3933
Size: 6896
SanDI GG'GwC_CC
Cuts at: 482 1459 2476 482
Size: 977 1017 4902
Fragments arranged by size:
4902 1017 977
SapI GCTCTTCn'nnn_
Cuts at: 1186 1462 4919 5129 1186
Size: 276 3457 210 2953
Fragments arranged by size:
3457 2953 276 210
SbfI CC_TGCA'GG
Cuts at: 3943 3943
Size: 6896
ScaI AGT'ACT
Cuts at: 6413 6413
Size: 6896
SfcI C'TryA_G
Cuts at: 33 1233 1422 2202 3201 3329 3597 3939 4624
Size: 1200 189 780 999 128 268 342 685
Cuts at: 4624 5617 5808 33
Size: 993 191 1121
Fragments arranged by size:
1200 1121 999 993 780 685 342 268
191 189 128
SfoI GGC'GCC
Cuts at: 1847 1991 2807 4328 4576 1847
Size: 144 816 1521 248 4167
Fragments arranged by size:
4167 1521 816 248 144
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
SmaI CCC'GGG
Cuts at: 3918 3918
Size: 6896
SmlI C'TyrA_G
Cuts at: 2087 2209 2677 4051 4966 5458 5720 2087
Size: 122 468 1374 915 492 262 3263
Fragments arranged by size:
3263 1374 915 492 468 262 122
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6896
SphI G_CATG'C
Cuts at: 3949 4449 3949
Size: 500 6396
SspI AAT'ATT
Cuts at: 645 645
Size: 6896
StuI AGG'CCT
Cuts at: 3199 3199
Size: 6896
StyI C'CwwG_G
Cuts at: 107 293 1662 2153 2592 2621 2956 3699 3766
Size: 186 1369 491 439 29 335 743 67
Cuts at: 3766 4030 4384 107
Size: 264 354 2619
Fragments arranged by size:
2619 1369 743 491 439 354 335 264
186 67 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 1333 3378 4479 4612 5071 5280 419
Size: 914 2045 1101 133 459 209 2035
Fragments arranged by size:
2045 2035 1101 914 459 209 133
TatI w'GTAC_w
Cuts at: 6411 6411
Size: 6896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1900 1928 1932 1960 2874 2906 3010 3042 6413
Size: 28 4 28 914 32 104 32 3371
Cuts at: 6413 6445 1900
Size: 32 2351
Fragments arranged by size:
3371 2351 914 104 32 32 32 28
28 4
Tth111I GACn'n_nGTC
Cuts at: 1281 1354 4693 1281
Size: 73 3339 3484
Fragments arranged by size:
3484 3339 73
XbaI T'CTAG_A
Cuts at: 3927 3927
Size: 6896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1438 2625 2706 4391 1438
Size: 1187 81 1685 3943
Fragments arranged by size:
3943 1685 1187 81
XmaI C'CCGG_G
Cuts at: 3916 3916
Size: 6896
XmnI GAAnn'nnTTC
Cuts at: 518 3388 518
Size: 2870 4026
ZraI GAC'GTC
Cuts at: 300 1926 300
Size: 1626 5270
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI BaeI BfrBI BmgBI BmtI
BsiWI BsmI BsrGI BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI