Restriction map for
pF1KA0651
(Circular) (Six-base) MAPSORT of: pF1KA0651.seq Check: 9568 from: 1 to: 6353
pF1KA0651 6353 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 3391 3391
Size: 6353
Acc65I G'GTAC_C
Cuts at: 388 3369 388
Size: 2981 3372
AclI AA'CG_TT
Cuts at: 6193 6193
Size: 6353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 709 1171 1928 4178 4610 5357 5625 405
Size: 304 462 757 2250 432 747 268 1133
Fragments arranged by size:
2250 1133 757 747 462 432 304 268
AflIII A'CryG_T
Cuts at: 3705 4809 5786 3705
Size: 1104 977 4272
Fragments arranged by size:
4272 1104 977
AgeI A'CCGG_T
Cuts at: 4741 4741
Size: 6353
AleI CACnn'nnGTG
Cuts at: 182 182
Size: 6353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1081 1198 1081
Size: 117 6236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2671 2703 2671
Size: 32 6321
AlwNI CAG_nnn'CTG
Cuts at: 1224 1302 1632 1762 3177 3658 5225 1224
Size: 78 330 130 1415 481 1567 2352
Fragments arranged by size:
2352 1567 1415 481 330 130 78
ApaI G_GGCC'C
Cuts at: 1710 1749 1774 2701 3018 1710
Size: 39 25 927 317 5045
Fragments arranged by size:
5045 927 317 39 25
ApaLI G'TGCA_C
Cuts at: 2041 5123 2041
Size: 3082 3271
ApoI r'AATT_y
Cuts at: 3357 5706 5962 3357
Size: 2349 256 3748
Fragments arranged by size:
3748 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6353
AvaI C'yCGr_G
Cuts at: 392 432 458 1460 1742 2054 2429 2735 2924
Size: 40 26 1002 282 312 375 306 189
Cuts at: 2924 3373 392
Size: 449 3372
Fragments arranged by size:
3372 1002 449 375 312 306 282 189
40 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 380 413 1519 1552 380
Size: 33 1106 33 5181
Fragments arranged by size:
5181 1106 33 33
BamHI G'GATC_C
Cuts at: 899 2075 3030 3378 899
Size: 1176 955 348 3874
Fragments arranged by size:
3874 1176 955 348
BanI G'GyrC_C
Cuts at: 388 509 3369 3783 4031 4066 388
Size: 121 2860 414 248 35 2675
Fragments arranged by size:
2860 2675 414 248 121 35
BanII G_rGCy'C
Cuts at: 802 1247 1710 1749 1774 2701 3018 3187 3367
Size: 445 463 39 25 927 317 169 180
Cuts at: 3367 4397 802
Size: 1030 2758
Fragments arranged by size:
2758 1030 927 463 445 317 180 169
39 25
BbeI G_GCGC'C
Cuts at: 3787 4035 3787
Size: 248 6105
BbsI GAAGACnn'nnnn_
Cuts at: 1840 1840
Size: 6353
BbvCI CC'TCA_GC
Cuts at: 1270 3002 1270
Size: 1732 4621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6319
BciVI GTATCCnnnnn_n'
Cuts at: 306 339 4251 5012 6353 306
Size: 33 3912 761 1341 306
Fragments arranged by size:
3912 1341 761 306 33
BclI T'GATC_A
Cuts at: 2009 3873 2009
Size: 1864 4489
BglI GCCn_nnn'nGGC
Cuts at: 1778 2773 2953 3013 1778
Size: 995 180 60 5118
Fragments arranged by size:
5118 995 180 60
BglII A'GATC_T
Cuts at: 1721 3868 5905 1721
Size: 2147 2037 2169
Fragments arranged by size:
2169 2147 2037
BlpI GC'TnA_GC
Cuts at: 3465 3465
Size: 6353
Bme1580I G_kGCm'C
Cuts at: 512 1309 1710 1749 1774 2045 2199 2701 2931
Size: 797 401 39 25 271 154 502 230
Cuts at: 2931 3018 3978 4071 5127 512
Size: 87 960 93 1056 1738
Fragments arranged by size:
1738 1056 960 797 502 401 271 230
154 93 87 39 25
BmrI ACTGGGnnnn_n'
Cuts at: 327 1654 1750 3187 3565 3646 3981 6125 327
Size: 1327 96 1437 378 81 335 2144 555
Fragments arranged by size:
2144 1437 1327 555 378 335 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4720 4720
Size: 6353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 1882 2914 2956 1717
Size: 165 1032 42 5114
Fragments arranged by size:
5114 1032 165 42
Bpu10I CC'TnA_GC
Cuts at: 1270 2702 3002 1270
Size: 1432 300 4621
Fragments arranged by size:
4621 1432 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2347 3093 3529 4408 4900 5198 1498
Size: 849 746 436 879 492 298 2653
Fragments arranged by size:
2653 879 849 746 492 436 298
BsaI GGTCTCn'nnnn_
Cuts at: 6057 6057
Size: 6353
BsaAI yAC'GTr
Cuts at: 3706 3706
Size: 6353
BsaBI GATnn'nnATC
Cuts at: 6 3867 5904 6
Size: 3861 2037 455
Fragments arranged by size:
3861 2037 455
BsaHI Gr'CG_yC
Cuts at: 1293 3784 4032 4734 6230 1293
Size: 2491 248 702 1496 1416
Fragments arranged by size:
2491 1496 1416 702 248
BsaWI w'CCGG_w
Cuts at: 442 1487 2063 3542 4063 4741 5015 5162 5573
Size: 1045 576 1479 521 678 274 147 411
Cuts at: 5573 442
Size: 1222
Fragments arranged by size:
1479 1222 1045 678 576 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1375 1405 1651 1681 2704 2734 1375
Size: 30 246 30 1023 30 4994
Fragments arranged by size:
4994 1023 246 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 393 807 1630 1633 2611 2851 3247 3319 393
Size: 414 823 3 978 240 396 72 3427
Fragments arranged by size:
3427 978 823 414 396 240 72 3
BseYI C'CCAG_C
Cuts at: 2660 2758 2997 3094 3778 4536 5113 5609 2660
Size: 98 239 97 684 758 577 496 3404
Fragments arranged by size:
3404 758 684 577 496 239 98 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 6353
BsiEI CG_ry'CG
Cuts at: 104 3895 3941 5149 104
Size: 3791 46 1208 1308
Fragments arranged by size:
3791 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1247 1514 2045 2071 3367 4145 4335 5127 1247
Size: 267 531 26 1296 778 190 792 2473
Fragments arranged by size:
2473 1296 792 778 531 267 190 26
BsiWI C'GTAC_G
Cuts at: 1971 1971
Size: 6353
BsmI GAATG_Cn'
Cuts at: 1599 3293 1599
Size: 1694 4659
BsmBI CGTCTCn'nnnn_
Cuts at: 3301 3301
Size: 6353
Bsp1286I G_dGCh'C
Cuts at: 512 802 1247 1309 1514 1710 1749 1774 2045
Size: 290 445 62 205 196 39 25 271
Cuts at: 2045 2071 2199 2701 2931 3018 3187 3367 3978
Size: 26 128 502 230 87 169 180 611
Cuts at: 3978 4071 4145 4335 4397 5127 512
Size: 93 74 190 62 730 1738
Fragments arranged by size:
1738 730 611 502 445 290 271 230
205 196 190 180 169 128 93 87
74 62 62 39 26 25
BspEI T'CCGG_A
Cuts at: 1487 5573 1487
Size: 4086 2267
BspHI T'CATG_A
Cuts at: 1157 5529 1157
Size: 4372 1981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 2165 2399 3403 3617 3919 4300 610
Size: 1555 234 1004 214 302 381 2663
Fragments arranged by size:
2663 1555 1004 381 302 234 214
BsrBI CCG'CTC
Cuts at: 50 747 815 2315 2346 2706 3195 4645 4699
Size: 697 68 1500 31 360 489 1450 54
Cuts at: 4699 6183 6348 50
Size: 1484 165 55
Fragments arranged by size:
1500 1484 1450 697 489 360 165 68
55 54 31
BsrDI GCAATG_nn'
Cuts at: 612 854 1979 4265 612
Size: 242 1125 2286 2700
Fragments arranged by size:
2700 2286 1125 242
BsrFI r'CCGG_y
Cuts at: 2852 4351 4532 4741 2852
Size: 1499 181 209 4464
Fragments arranged by size:
4464 1499 209 181
BssSI C'ACGA_G
Cuts at: 2336 2472 3272 4624 4982 2336
Size: 136 800 1352 358 3707
Fragments arranged by size:
3707 1352 800 358 136
BstAPI GCAn_nnn'nTGC
Cuts at: 2097 3061 3730 2097
Size: 964 669 4720
Fragments arranged by size:
4720 964 669
BstBI TT'CG_AA
Cuts at: 723 4714 723
Size: 3991 2362
BstXI CCAn_nnnn'nTGG
Cuts at: 1644 2799 1644
Size: 1155 5198
BstYI r'GATC_y
Cuts at: 1 141 899 1721 2075 3030 3117 3378 3845
Size: 140 758 822 354 955 87 261 467
Cuts at: 3845 3868 4203 4449 5461 5547 5559 5905 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 955 822 758 467 449 354 346
335 261 246 140 87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 352 2693 352
Size: 2341 4012
BtgI C'CryG_G
Cuts at: 807 950 1605 2792 807
Size: 143 655 1187 4368
Fragments arranged by size:
4368 1187 655 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1321 3074 4303 4484 4647 1321
Size: 1753 1229 181 163 3027
Fragments arranged by size:
3027 1753 1229 181 163
BtsI GCAGTG_nn'
Cuts at: 2280 2486 2929 3216 3596 2280
Size: 206 443 287 380 5037
Fragments arranged by size:
5037 443 380 287 206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 837 872 837
Size: 35 6318
DraI TTT'AAA
Cuts at: 3352 3352
Size: 6353
DraIII CAC_nnn'GTG
Cuts at: 1140 1140
Size: 6353
DrdI GACnn_nn'nnGTC
Cuts at: 4059 4917 4059
Size: 858 5495
EaeI y'GGCC_r
Cuts at: 248 748 758 810 1779 2969 3938 4112 4503
Size: 500 10 52 969 1190 969 174 391
Cuts at: 4503 4530 5875 248
Size: 27 1345 726
Fragments arranged by size:
1345 1190 969 969 726 500 391 174
52 27 10
EagI C'GGCC_G
Cuts at: 3938 3938
Size: 6353
EarI CTCTTCn'nnn_
Cuts at: 137 520 725 965 1174 1184 2255 2945 3185
Size: 383 205 240 209 10 1071 690 240
Cuts at: 3185 4376 4586 137
Size: 1191 210 1904
Fragments arranged by size:
1904 1191 1071 690 383 240 240 210
209 205 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3248 4561 4871 5017 6163 3248
Size: 1313 310 146 1146 3438
Fragments arranged by size:
3438 1313 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1245 3365 1245
Size: 2120 4233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 709 1171 1717 1882 1928 2914 2956 4178
Size: 304 462 546 165 46 986 42 1222
Cuts at: 4178 4610 5357 5625 405
Size: 432 747 268 1133
Fragments arranged by size:
1222 1133 986 747 546 462 432 304
268 165 46 42
EcoO109I rG'GnC_Cy
Cuts at: 116 127 1221 1299 1372 1629 1706 1770 1771
Size: 11 1094 78 73 257 77 64 1
Cuts at: 1771 2304 2697 2698 3006 3492 116
Size: 533 393 1 308 486 2977
Fragments arranged by size:
2977 1094 533 486 393 308 257 78
77 73 64 11 1 1
EcoRI G'AATT_C
Cuts at: 3357 3357
Size: 6353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1791 1938 4372 1791
Size: 147 2434 3772
Fragments arranged by size:
3772 2434 147
FspI TGC'GCA
Cuts at: 2101 4134 2101
Size: 2033 4320
FspAI rTGC'GCAy
Cuts at: 2101 2101
Size: 6353
HaeII r_GCGC'y
Cuts at: 1626 1851 2850 3787 4035 5057 6036 1626
Size: 225 999 937 248 1022 979 1943
Fragments arranged by size:
1943 1022 999 979 937 248 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1651 1683 2207 2239 2533 2565 4195 4225 4227
Size: 32 524 32 294 32 1630 30 2
Cuts at: 4227 4257 1651
Size: 30 3747
Fragments arranged by size:
3747 1630 524 294 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3392 3392
Size: 6353
KasI G'GCGC_C
Cuts at: 3783 4031 3783
Size: 248 6105
KpnI G_GTAC'C
Cuts at: 392 3373 392
Size: 2981 3372
MluI A'CGCG_T
Cuts at: 5786 5786
Size: 6353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 5024 5208 797
Size: 4227 184 1942
Fragments arranged by size:
4227 1942 184
MscI TGG'CCA
Cuts at: 4114 4114
Size: 6353
MslI CAynn'nnrTG
Cuts at: 182 256 544 869 1180 2674 4342 4469 182
Size: 74 288 325 311 1494 1668 127 2066
Fragments arranged by size:
2066 1668 1494 325 311 288 127 74
MspA1I CmG'CkG
Cuts at: 372 626 1030 1695 2462 2580 2653 2814 2967
Size: 254 404 665 767 118 73 161 153
Cuts at: 2967 3001 3464 3778 4138 5151 5396 372
Size: 34 463 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 767 665 463 404 360 314
254 245 161 153 118 73 34
NaeI GCC'GGC
Cuts at: 2854 4534 2854
Size: 1680 4673
NarI GG'CG_CC
Cuts at: 3784 4032 3784
Size: 248 6105
NgoMIV G'CCGG_C
Cuts at: 2852 4532 2852
Size: 1680 4673
NspI r_CATG'y
Cuts at: 261 933 2108 2824 3406 3906 4813 261
Size: 672 1175 716 582 500 907 1801
Fragments arranged by size:
1801 1175 907 716 672 582 500
PasI CC'CwG_GG
Cuts at: 885 2300 2976 3011 885
Size: 1415 676 35 4227
Fragments arranged by size:
4227 1415 676 35
PciI A'CATG_T
Cuts at: 4809 4809
Size: 6353
PflMI CCAn_nnn'nTGG
Cuts at: 229 1341 1762 229
Size: 1112 421 4820
Fragments arranged by size:
4820 1112 421
PfoI T'CCnGG_A
Cuts at: 740 1898 2906 3299 740
Size: 1158 1008 393 3794
Fragments arranged by size:
3794 1158 1008 393
PmeI GTTT'AAAC
Cuts at: 3352 3352
Size: 6353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5492 5524 5492
Size: 32 6321
PpuMI rG'GwC_Cy
Cuts at: 1372 1629 1372
Size: 257 6096
PspOMI G'GGCC_C
Cuts at: 1706 1745 1770 2697 3014 1706
Size: 39 25 927 317 5045
Fragments arranged by size:
5045 927 317 39 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 6321
PstI C_TGCA'G
Cuts at: 141 367 1070 1417 1598 1718 1961 2357 2536
Size: 226 703 347 181 120 243 396 179
Cuts at: 2536 2813 2834 3400 4085 141
Size: 277 21 566 685 2409
Fragments arranged by size:
2409 703 685 566 396 347 277 243
226 181 179 120 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6353
PvuII CAG'CTG
Cuts at: 372 1030 1695 2580 2653 2967 3001 3778 4138
Size: 658 665 885 73 314 34 777 360
Cuts at: 4138 372
Size: 2587
Fragments arranged by size:
2587 885 777 665 658 360 314 73
34
RsrII CG'GwC_CG
Cuts at: 4548 4548
Size: 6353
SacI G_AGCT'C
Cuts at: 1247 3367 1247
Size: 2120 4233
SalI G'TCGA_C
Cuts at: 3390 3390
Size: 6353
SapI GCTCTTCn'nnn_
Cuts at: 1184 3185 4376 4586 1184
Size: 2001 1191 210 2951
Fragments arranged by size:
2951 2001 1191 210
SbfI CC_TGCA'GG
Cuts at: 3400 3400
Size: 6353
ScaI AGT'ACT
Cuts at: 5870 5870
Size: 6353
SfcI C'TryA_G
Cuts at: 33 137 363 981 1066 1413 1594 1714 1957
Size: 104 226 618 85 347 181 120 243
Cuts at: 1957 2353 2532 2644 2809 2830 3332 3396 4081
Size: 396 179 112 165 21 502 64 685
Cuts at: 4081 5074 5265 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 618 502 396 347 243
226 191 181 179 165 120 112 104
85 64 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 1778 3013 1778
Size: 1235 5118
SfoI GGC'GCC
Cuts at: 3785 4033 3785
Size: 248 6105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6353
SmaI CCC'GGG
Cuts at: 394 460 2056 2926 3375 394
Size: 66 1596 870 449 3372
Fragments arranged by size:
3372 1596 870 449 66
SmlI C'TyrA_G
Cuts at: 1513 2326 3072 3508 4423 4915 5177 1513
Size: 813 746 436 915 492 262 2689
Fragments arranged by size:
2689 915 813 746 492 436 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6353
SphI G_CATG'C
Cuts at: 261 3406 3906 261
Size: 3145 500 2708
Fragments arranged by size:
3145 2708 500
SrfI GCCC'GGGC
Cuts at: 2926 2926
Size: 6353
StuI AGG'CCT
Cuts at: 1432 1432
Size: 6353
StyI C'CwwG_G
Cuts at: 120 485 578 653 2447 3487 3841 120
Size: 365 93 75 1794 1040 354 2632
Fragments arranged by size:
2632 1794 1040 365 354 93 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1333 3001 3176 3936 4069 4528 4737 1333
Size: 1668 175 760 133 459 209 2949
Fragments arranged by size:
2949 1668 760 459 209 175 133
TatI w'GTAC_w
Cuts at: 1884 1916 3068 5868 1884
Size: 32 1152 2800 2369
Fragments arranged by size:
2800 2369 1152 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1375 1407 2035 2067 2704 2736 5870 5902 1375
Size: 32 628 32 637 32 3134 32 1826
Fragments arranged by size:
3134 1826 637 628 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4150 4150
Size: 6353
XbaI T'CTAG_A
Cuts at: 3384 3384
Size: 6353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 250 2370 3848 250
Size: 2120 1478 2755
Fragments arranged by size:
2755 2120 1478
XmaI C'CCGG_G
Cuts at: 392 458 2054 2924 3373 392
Size: 66 1596 870 449 3372
Fragments arranged by size:
3372 1596 870 449 66
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BfrBI
BmgBI BmtI BsrGI BssHII BstEII BstZ17I ClaI EcoNI
EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
SacII SanDI SexAI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI