Restriction map for
pF1KA0656
(Circular) (Six-base) MAPSORT of: pF1KA0656.seq Check: 8640 from: 1 to: 5834
pF1KA0656 5834 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1230 2766 1230
Size: 1536 4298
AccI GT'mk_AC
Cuts at: 469 2872 469
Size: 2403 3431
Acc65I G'GTAC_C
Cuts at: 2328 2422 2850 2328
Size: 94 428 5312
Fragments arranged by size:
5312 428 94
AclI AA'CG_TT
Cuts at: 5674 5674
Size: 5834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1433 1577 1844 1997 2431 3659 4091 4838
Size: 117 144 267 153 434 1228 432 747
Cuts at: 4838 5106 1316
Size: 268 2044
Fragments arranged by size:
2044 1228 747 434 432 268 267 153
144 117
AfeI AGC'GCT
Cuts at: 2055 2055
Size: 5834
AflIII A'CryG_T
Cuts at: 3186 4290 5267 3186
Size: 1104 977 3753
Fragments arranged by size:
3753 1104 977
AgeI A'CCGG_T
Cuts at: 1086 4222 1086
Size: 3136 2698
AlwNI CAG_nnn'CTG
Cuts at: 166 320 1375 1573 1702 1852 3139 4706 166
Size: 154 1055 198 129 150 1287 1567 1294
Fragments arranged by size:
1567 1294 1287 1055 198 154 150 129
ApaI G_GGCC'C
Cuts at: 217 2598 217
Size: 2381 3453
ApaLI G'TGCA_C
Cuts at: 2540 4604 2540
Size: 2064 3770
ApoI r'AATT_y
Cuts at: 646 812 853 2414 2838 5187 5443 646
Size: 166 41 1561 424 2349 256 1037
Fragments arranged by size:
2349 1561 1037 424 256 166 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 5834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5834
AvaI C'yCGr_G
Cuts at: 2854 2854
Size: 5834
BamHI G'GATC_C
Cuts at: 448 2859 448
Size: 2411 3423
BanI G'GyrC_C
Cuts at: 2142 2295 2328 2422 2850 3264 3512 3547 2142
Size: 153 33 94 428 414 248 35 4429
Fragments arranged by size:
4429 428 414 248 153 94 35 33
BanII G_rGCy'C
Cuts at: 217 2598 2848 3878 217
Size: 2381 250 1030 2173
Fragments arranged by size:
2381 2173 1030 250
BbeI G_GCGC'C
Cuts at: 2146 3268 3516 2146
Size: 1122 248 4464
Fragments arranged by size:
4464 1122 248
BbsI GAAGACnn'nnnn_
Cuts at: 1013 1013
Size: 5834
BbvCI CC'TCA_GC
Cuts at: 1368 2666 1368
Size: 1298 4536
BciVI GTATCCnnnnn_n'
Cuts at: 3732 4493 5834 3732
Size: 761 1341 3732
Fragments arranged by size:
3732 1341 761
BclI T'GATC_A
Cuts at: 3354 3354
Size: 5834
BfrBI ATG'CAT
Cuts at: 1525 1960 2041 1525
Size: 435 81 5318
Fragments arranged by size:
5318 435 81
BglII A'GATC_T
Cuts at: 714 2442 3349 5386 714
Size: 1728 907 2037 1162
Fragments arranged by size:
2037 1728 1162 907
BlpI GC'TnA_GC
Cuts at: 2946 2946
Size: 5834
Bme1580I G_kGCm'C
Cuts at: 217 2544 2598 3459 3552 4608 217
Size: 2327 54 861 93 1056 1443
Fragments arranged by size:
2327 1443 1056 861 93 54
BmrI ACTGGGnnnn_n'
Cuts at: 1117 2330 3046 3127 3462 5606 1117
Size: 1213 716 81 335 2144 1345
Fragments arranged by size:
2144 1345 1213 716 335 81
BmtI G_CTAG'C
Cuts at: 1646 1646
Size: 5834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4201 4201
Size: 5834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1147 1202 1247 2141 2150 2480 2492 2636
Size: 137 55 45 894 9 330 12 144
Cuts at: 2636 2768 1010
Size: 132 4076
Fragments arranged by size:
4076 894 330 144 137 132 55 45
12 9
Bpu10I CC'TnA_GC
Cuts at: 1368 1580 1841 2666 1368
Size: 212 261 825 4536
Fragments arranged by size:
4536 825 261 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 3010 3889 4381 4679 844
Size: 2166 879 492 298 1999
Fragments arranged by size:
2166 1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 253 1241 1606 5538 253
Size: 988 365 3932 549
Fragments arranged by size:
3932 988 549 365
BsaAI yAC'GTr
Cuts at: 3187 3187
Size: 5834
BsaBI GATnn'nnATC
Cuts at: 6 656 3348 5385 6
Size: 650 2692 2037 455
Fragments arranged by size:
2692 2037 650 455
BsaHI Gr'CG_yC
Cuts at: 2143 3265 3513 4215 5711 2143
Size: 1122 248 702 1496 2266
Fragments arranged by size:
2266 1496 1122 702 248
BsaWI w'CCGG_w
Cuts at: 1086 1656 3023 3544 4222 4496 4643 5054 1086
Size: 570 1367 521 678 274 147 411 1866
Fragments arranged by size:
1866 1367 678 570 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 570 600 900 930 1638 1668 1761 1764 1791
Size: 30 300 30 708 30 93 3 27
Cuts at: 1791 1794 570
Size: 3 4610
Fragments arranged by size:
4610 708 300 93 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1244 1769 1881 1244
Size: 525 112 5197
Fragments arranged by size:
5197 525 112
BseYI C'CCAG_C
Cuts at: 320 919 2302 2641 3259 4017 4594 5090 320
Size: 599 1383 339 618 758 577 496 1064
Fragments arranged by size:
1383 1064 758 618 599 577 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1259 1559 1586 2297 1259
Size: 300 27 711 4796
Fragments arranged by size:
4796 711 300 27
BsiEI CG_ry'CG
Cuts at: 104 3376 3422 4630 104
Size: 3272 46 1208 1308
Fragments arranged by size:
3272 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1002 2544 2848 3626 3816 4608 1002
Size: 1542 304 778 190 792 2228
Fragments arranged by size:
2228 1542 792 778 304 190
BsmI GAATG_Cn'
Cuts at: 2460 2636 2460
Size: 176 5658
BsmBI CGTCTCn'nnnn_
Cuts at: 928 1049 928
Size: 121 5713
Bsp1286I G_dGCh'C
Cuts at: 217 1002 2544 2598 2848 3459 3552 3626 3816
Size: 785 1542 54 250 611 93 74 190
Cuts at: 3816 3878 4608 217
Size: 62 730 1443
Fragments arranged by size:
1542 1443 785 730 611 250 190 93
74 62 54
BspEI T'CCGG_A
Cuts at: 5054 5054
Size: 5834
BspHI T'CATG_A
Cuts at: 201 5010 201
Size: 4809 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1230 2079 2189 2766 2884 3098 3400 3781 1230
Size: 849 110 577 118 214 302 381 3283
Fragments arranged by size:
3283 849 577 381 302 214 118 110
BsrBI CCG'CTC
Cuts at: 50 145 181 303 4126 4180 5664 5829 50
Size: 95 36 122 3823 54 1484 165 55
Fragments arranged by size:
3823 1484 165 122 95 55 54 36
BsrDI GCAATG_nn'
Cuts at: 1604 2351 3746 1604
Size: 747 1395 3692
Fragments arranged by size:
3692 1395 747
BsrFI r'CCGG_y
Cuts at: 1086 2673 3832 4013 4222 1086
Size: 1587 1159 181 209 2698
Fragments arranged by size:
2698 1587 1159 209 181
BssSI C'ACGA_G
Cuts at: 842 4105 4463 842
Size: 3263 358 2213
Fragments arranged by size:
3263 2213 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1573 1702 1987 3211 1522
Size: 51 129 285 1224 4145
Fragments arranged by size:
4145 1224 285 129 51
BstBI TT'CG_AA
Cuts at: 4195 4195
Size: 5834
BstXI CCAn_nnnn'nTGG
Cuts at: 2516 2516
Size: 5834
BstYI r'GATC_y
Cuts at: 1 448 714 991 1311 1419 2442 2805 2859
Size: 447 266 277 320 108 1023 363 54
Cuts at: 2859 3326 3349 3684 3930 4942 5028 5040 5386
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5386 1
Size: 449
Fragments arranged by size:
1023 1012 467 449 447 363 346 335
320 277 266 246 108 86 54 23
12
BtgI C'CryG_G
Cuts at: 453 2291 453
Size: 1838 3996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3784 3965 4128 3784
Size: 181 163 5490
Fragments arranged by size:
5490 181 163
BtsI GCAGTG_nn'
Cuts at: 637 1235 1406 1772 1934 1985 3077 637
Size: 598 171 366 162 51 1092 3394
Fragments arranged by size:
3394 1092 598 366 171 162 51
DraI TTT'AAA
Cuts at: 2833 2833
Size: 5834
DraIII CAC_nnn'GTG
Cuts at: 2539 2539
Size: 5834
DrdI GACnn_nn'nnGTC
Cuts at: 846 3540 4398 846
Size: 2694 858 2282
Fragments arranged by size:
2694 2282 858
EaeI y'GGCC_r
Cuts at: 284 3419 3593 3984 4011 5356 284
Size: 3135 174 391 27 1345 762
Fragments arranged by size:
3135 1345 762 391 174 27
EagI C'GGCC_G
Cuts at: 3419 3419
Size: 5834
EarI CTCTTCn'nnn_
Cuts at: 3857 4067 3857
Size: 210 5624
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4042 4352 4498 5644 4042
Size: 310 146 1146 4232
Fragments arranged by size:
4232 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2846 2846
Size: 5834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1147 1202 1247 1316 1433 1577 1844 1997
Size: 137 55 45 69 117 144 267 153
Cuts at: 1997 2141 2150 2431 2480 2492 2636 2768 3659
Size: 144 9 281 49 12 144 132 891
Cuts at: 3659 4091 4838 5106 1010
Size: 432 747 268 1738
Fragments arranged by size:
1738 891 747 432 281 268 267 153
144 144 144 137 132 117 69 55
49 45 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 477 910 1226 2549 2618 477
Size: 433 316 1323 69 3693
Fragments arranged by size:
3693 1323 433 316 69
EcoO109I rG'GnC_Cy
Cuts at: 214 1458 1907 2594 2595 2702 2793 2973 214
Size: 1244 449 687 1 107 91 180 3075
Fragments arranged by size:
3075 1244 687 449 180 107 91 1
EcoRI G'AATT_C
Cuts at: 2838 2838
Size: 5834
EcoRV GAT'ATC
Cuts at: 1794 1794
Size: 5834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1037 2072 2561 2841 3853 1037
Size: 1035 489 280 1012 3018
Fragments arranged by size:
3018 1035 1012 489 280
FspI TGC'GCA
Cuts at: 3615 3615
Size: 5834
HaeII r_GCGC'y
Cuts at: 486 1840 2057 2146 3268 3516 4538 5517 486
Size: 1354 217 89 1122 248 1022 979 803
Fragments arranged by size:
1354 1122 1022 979 803 248 217 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 339 371 570 602 1162 1194 1296 1328 1873
Size: 32 199 32 560 32 102 32 545
Cuts at: 1873 1905 1933 1965 2154 2186 3676 3706 3708
Size: 32 28 32 189 32 1490 30 2
Cuts at: 3708 3738 339
Size: 30 2435
Fragments arranged by size:
2435 1490 560 545 199 189 102 32
32 32 32 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 2474 2873 2474
Size: 399 5435
KasI G'GCGC_C
Cuts at: 2142 3264 3512 2142
Size: 1122 248 4464
Fragments arranged by size:
4464 1122 248
KpnI G_GTAC'C
Cuts at: 2332 2426 2854 2332
Size: 94 428 5312
Fragments arranged by size:
5312 428 94
MluI A'CGCG_T
Cuts at: 5267 5267
Size: 5834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2586 2590 4505 4689 2586
Size: 4 1915 184 3731
Fragments arranged by size:
3731 1915 184 4
MscI TGG'CCA
Cuts at: 3595 3595
Size: 5834
MslI CAynn'nnrTG
Cuts at: 374 458 662 2656 3823 3950 374
Size: 84 204 1994 1167 127 2258
Fragments arranged by size:
2258 1994 1167 204 127 84
MspA1I CmG'CkG
Cuts at: 1699 1732 1759 2302 2722 2945 3259 3619 4632
Size: 33 27 543 420 223 314 360 1013
Cuts at: 4632 4877 1699
Size: 245 2656
Fragments arranged by size:
2656 1013 543 420 360 314 245 223
33 27
NaeI GCC'GGC
Cuts at: 4015 4015
Size: 5834
NarI GG'CG_CC
Cuts at: 2143 3265 3513 2143
Size: 1122 248 4464
Fragments arranged by size:
4464 1122 248
NcoI C'CATG_G
Cuts at: 453 2291 453
Size: 1838 3996
NgoMIV G'CCGG_C
Cuts at: 4013 4013
Size: 5834
NheI G'CTAG_C
Cuts at: 1642 1642
Size: 5834
NsiI A_TGCA'T
Cuts at: 1527 1962 2043 1527
Size: 435 81 5318
Fragments arranged by size:
5318 435 81
NspI r_CATG'y
Cuts at: 1529 2655 2887 3387 4294 1529
Size: 1126 232 500 907 3069
Fragments arranged by size:
3069 1126 907 500 232
PciI A'CATG_T
Cuts at: 4290 4290
Size: 5834
PflMI CCAn_nnn'nTGG
Cuts at: 1225 1241 2605 1225
Size: 16 1364 4454
Fragments arranged by size:
4454 1364 16
PfoI T'CCnGG_A
Cuts at: 1154 1154
Size: 5834
PmeI GTTT'AAAC
Cuts at: 2833 2833
Size: 5834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4973 5005 4973
Size: 32 5802
PpuMI rG'GwC_Cy
Cuts at: 2793 2793
Size: 5834
PspOMI G'GGCC_C
Cuts at: 213 2594 213
Size: 2381 3453
PstI C_TGCA'G
Cuts at: 1467 1836 2881 3566 1467
Size: 369 1045 685 3735
Fragments arranged by size:
3735 1045 685 369
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5834
PvuII CAG'CTG
Cuts at: 1699 2302 3259 3619 1699
Size: 603 957 360 3914
Fragments arranged by size:
3914 957 603 360
RsrII CG'GwC_CG
Cuts at: 4029 4029
Size: 5834
SacI G_AGCT'C
Cuts at: 2848 2848
Size: 5834
SalI G'TCGA_C
Cuts at: 2871 2871
Size: 5834
SapI GCTCTTCn'nnn_
Cuts at: 3857 4067 3857
Size: 210 5624
SbfI CC_TGCA'GG
Cuts at: 2881 2881
Size: 5834
ScaI AGT'ACT
Cuts at: 5351 5351
Size: 5834
SexAI A'CCwGG_T
Cuts at: 2515 2515
Size: 5834
SfcI C'TryA_G
Cuts at: 33 170 486 619 1463 1505 1625 1637 1832
Size: 137 316 133 844 42 120 12 195
Cuts at: 1832 2185 2877 3562 4555 4746 33
Size: 353 692 685 993 191 1121
Fragments arranged by size:
1121 993 844 692 685 353 316 195
191 137 133 120 42 12
SfoI GGC'GCC
Cuts at: 2144 3266 3514 2144
Size: 1122 248 4464
Fragments arranged by size:
4464 1122 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5834
SmaI CCC'GGG
Cuts at: 2856 2856
Size: 5834
SmlI C'TyrA_G
Cuts at: 859 2989 3904 4396 4658 859
Size: 2130 915 492 262 2035
Fragments arranged by size:
2130 2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5834
SphI G_CATG'C
Cuts at: 2655 2887 3387 2655
Size: 232 500 5102
Fragments arranged by size:
5102 500 232
SspI AAT'ATT
Cuts at: 273 394 273
Size: 121 5713
StyI C'CwwG_G
Cuts at: 453 2291 2364 2968 3322 453
Size: 1838 73 604 354 2965
Fragments arranged by size:
2965 1838 604 354 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 312 2349 3417 3550 4009 4218 312
Size: 2037 1068 133 459 209 1928
Fragments arranged by size:
2037 1928 1068 459 209 133
TatI w'GTAC_w
Cuts at: 149 1876 5349 149
Size: 1727 3473 634
Fragments arranged by size:
3473 1727 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 932 1638 1670 1761 1764 1793 1796 5351
Size: 32 706 32 91 3 29 3 3555
Cuts at: 5351 5383 900
Size: 32 1351
Fragments arranged by size:
3555 1351 706 91 32 32 32 29
3 3
Tth111I GACn'n_nGTC
Cuts at: 3631 3631
Size: 5834
XbaI T'CTAG_A
Cuts at: 403 807 1788 2865 403
Size: 404 981 1077 3372
Fragments arranged by size:
3372 1077 981 404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 927 2343 3329 927
Size: 1416 986 3432
Fragments arranged by size:
3432 1416 986
XmaI C'CCGG_G
Cuts at: 2854 2854
Size: 5834
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BglI BmgBI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI