Restriction map for
pF1KA0660
(Circular) (Six-base) MAPSORT of: pF1KA0660.seq Check: 6980 from: 1 to: 4559
pF1KA0660 4559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 1175 4399 1175
Size: 3224 1335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 476 746 833 2384 2816 3563 3831 300
Size: 176 270 87 1551 432 747 268 1028
Fragments arranged by size:
1551 1028 747 432 270 268 176 87
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 632 632
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 712 805 1864 3431 712
Size: 93 1059 1567 1840
Fragments arranged by size:
1840 1567 1059 93
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 216 335 475 899 1153 1224 1563 3912 4168
Size: 119 140 424 254 71 339 2349 256
Cuts at: 4168 216
Size: 607
Fragments arranged by size:
2349 607 424 339 256 254 140 119
71
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 1573 2603 1573
Size: 1030 3529
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 2184 2277 3333 2184
Size: 93 1056 3410
Fragments arranged by size:
3410 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 119 1771 1852 2187 4331 119
Size: 1652 81 335 2144 347
Fragments arranged by size:
2144 1652 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 701 701
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 1735 2614 3106 3404 384
Size: 1351 879 492 298 1539
Fragments arranged by size:
1539 1351 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4263 4263
Size: 4559
BsaAI yAC'GTr
Cuts at: 1420 1912 1420
Size: 492 4067
BsaBI GATnn'nnATC
Cuts at: 6 1097 2073 4110 6
Size: 1091 976 2037 455
Fragments arranged by size:
2037 1091 976 455
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 186 1748 2269 2947 3221 3368 3779 186
Size: 1562 521 678 274 147 411 966
Fragments arranged by size:
1562 966 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 365 395 774 777 804 807 365
Size: 30 379 3 27 3 4117
Fragments arranged by size:
4117 379 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 763 779 782 787 1055 1460 679
Size: 84 16 3 5 268 405 3778
Fragments arranged by size:
3778 405 268 84 16 5 3
BseYI C'CCAG_C
Cuts at: 1984 2742 3319 3815 1984
Size: 758 577 496 2728
Fragments arranged by size:
2728 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1573 2351 2541 3333 1573
Size: 778 190 792 2799
Fragments arranged by size:
2799 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1573 2184 2277 2351 2541 2603 3333 1573
Size: 611 93 74 190 62 730 2799
Fragments arranged by size:
2799 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 186 3779 186
Size: 3593 966
BspHI T'CATG_A
Cuts at: 687 1160 3735 687
Size: 473 2575 1511
Fragments arranged by size:
2575 1511 473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 875 1609 1823 2125 2506 875
Size: 734 214 302 381 2928
Fragments arranged by size:
2928 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1401 2471 1401
Size: 1070 3489
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 974 2830 3188 974
Size: 1856 358 2345
Fragments arranged by size:
2345 1856 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4559
BstYI r'GATC_y
Cuts at: 1 460 1584 2051 2074 2409 2655 3667 3753
Size: 459 1124 467 23 335 246 1012 86
Cuts at: 3753 3765 4111 1
Size: 12 346 449
Fragments arranged by size:
1124 1012 467 459 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4559
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 1313 1558 1313
Size: 245 4314
DraIII CAC_nnn'GTG
Cuts at: 648 976 648
Size: 328 4231
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 276 2144 2318 2709 2736 4081 276
Size: 1868 174 391 27 1345 754
Fragments arranged by size:
1868 1345 754 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 563 722 1316 1455 1503 2582 2792 563
Size: 159 594 139 48 1079 210 2330
Fragments arranged by size:
2330 1079 594 210 159 139 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 476 746 833 2384 2816 3563 3831 300
Size: 176 270 87 1551 432 747 268 1028
Fragments arranged by size:
1551 1028 747 432 270 268 176 87
EcoNI CCTnn'n_nnAGG
Cuts at: 1019 1019
Size: 4559
EcoO109I rG'GnC_Cy
Cuts at: 1405 1465 1698 1405
Size: 60 233 4266
Fragments arranged by size:
4266 233 60
EcoRI G'AATT_C
Cuts at: 216 899 1153 1563 216
Size: 683 254 410 3212
Fragments arranged by size:
3212 683 410 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1173 2578 1173
Size: 1405 3154
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1993 2241 3263 4242 1993
Size: 248 1022 979 2310
Fragments arranged by size:
2310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 365 397 535 567 694 726 763 795 1434
Size: 32 138 32 127 32 37 32 639
Cuts at: 1434 1466 2401 2431 2433 2463 365
Size: 32 935 30 2 30 2461
Fragments arranged by size:
2461 935 639 138 127 37 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
HindIII A'AGCT_T
Cuts at: 1216 1216
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3230 3414 3230
Size: 184 4375
MscI TGG'CCA
Cuts at: 278 2320 278
Size: 2042 2517
MslI CAynn'nnrTG
Cuts at: 112 347 2548 2675 112
Size: 235 2201 127 1996
Fragments arranged by size:
2201 1996 235 127
MspA1I CmG'CkG
Cuts at: 132 1670 1984 2344 3357 3602 132
Size: 1538 314 360 1013 245 1089
Fragments arranged by size:
1538 1089 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 107 107
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 1612 2112 3019 1612
Size: 500 907 3152
Fragments arranged by size:
3152 907 500
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PflMI CCAn_nnn'nTGG
Cuts at: 390 1228 1480 390
Size: 838 252 3469
Fragments arranged by size:
3469 838 252
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PmlI CAC'GTG
Cuts at: 1420 1420
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PpuMI rG'GwC_Cy
Cuts at: 1405 1465 1405
Size: 60 4499
PstI C_TGCA'G
Cuts at: 1606 2291 1606
Size: 685 3874
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 722 2582 2792 722
Size: 1860 210 2489
Fragments arranged by size:
2489 1860 210
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SexAI A'CCwGG_T
Cuts at: 1005 1005
Size: 4559
SfcI C'TryA_G
Cuts at: 33 1602 2287 3280 3471 33
Size: 1569 685 993 191 1121
Fragments arranged by size:
1569 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 363 1714 2629 3121 3383 363
Size: 1351 915 492 262 1539
Fragments arranged by size:
1539 1351 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
SspI AAT'ATT
Cuts at: 163 193 163
Size: 30 4529
StyI C'CwwG_G
Cuts at: 107 1199 1474 1693 2047 107
Size: 1092 275 219 354 2619
Fragments arranged by size:
2619 1092 354 275 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 2142 2275 2734 2943 1420
Size: 722 133 459 209 3036
Fragments arranged by size:
3036 722 459 209 133
TatI w'GTAC_w
Cuts at: 4074 4074
Size: 4559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1163 1195 4076 4108 1163
Size: 32 2881 32 1614
Fragments arranged by size:
2881 1614 32 32
Tth111I GACn'n_nGTC
Cuts at: 1547 2356 1547
Size: 809 3750
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
XmnI GAAnn'nnTTC
Cuts at: 1259 1259
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI