Restriction map for
pF1KA0676
(Circular) (Six-base) MAPSORT of: pF1KA0676.seq Check: 6076 from: 1 to: 6863
pF1KA0676 6863 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2199
Size: 6863
AccI GT'mk_AC
Cuts at: 317 2171 3683 3901 317
Size: 1854 1512 218 3279
Fragments arranged by size:
3279 1854 1512 218
Acc65I G'GTAC_C
Cuts at: 3879 3879
Size: 6863
AclI AA'CG_TT
Cuts at: 6703 6703
Size: 6863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 958 1495 1645 3388 4688 5120 5473 6135
Size: 405 537 150 1743 1300 432 353 662
Cuts at: 6135 553
Size: 1281
Fragments arranged by size:
1743 1300 1281 662 537 432 405 353
150
AflIII A'CryG_T
Cuts at: 715 3215 3560 4215 6015 6296 715
Size: 2500 345 655 1800 281 1282
Fragments arranged by size:
2500 1800 1282 655 345 281
AhdI GACnn_n'nnGTC
Cuts at: 3561 3561
Size: 6863
AleI CACnn'nnGTG
Cuts at: 1999 2348 1999
Size: 349 6514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2366 2366
Size: 6863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3799 3831 3799
Size: 32 6831
AlwNI CAG_nnn'CTG
Cuts at: 399 599 864 1470 2085 2250 2478 3246 3341
Size: 200 265 606 615 165 228 768 95
Cuts at: 3341 3534 3717 4168 5606 399
Size: 193 183 451 1438 1656
Fragments arranged by size:
1656 1438 768 615 606 451 265 228
200 193 183 165 95
ApaLI G'TGCA_C
Cuts at: 5701 5701
Size: 6863
ApoI r'AATT_y
Cuts at: 2425 3867 6216 6472 2425
Size: 1442 2349 256 2816
Fragments arranged by size:
2816 2349 1442 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6863
AvaI C'yCGr_G
Cuts at: 508 748 956 1702 1792 3883 508
Size: 240 208 746 90 2091 3488
Fragments arranged by size:
3488 2091 746 240 208 90
AvrII C'CTAG_G
Cuts at: 901 901
Size: 6863
BamHI G'GATC_C
Cuts at: 3888 3888
Size: 6863
BanI G'GyrC_C
Cuts at: 238 1449 3676 3879 4293 4541 4576 238
Size: 1211 2227 203 414 248 35 2525
Fragments arranged by size:
2525 2227 1211 414 248 203 35
BanII G_rGCy'C
Cuts at: 121 785 2013 2042 2405 2908 3520 3598 3877
Size: 664 1228 29 363 503 612 78 279
Cuts at: 3877 4907 121
Size: 1030 2077
Fragments arranged by size:
2077 1228 1030 664 612 503 363 279
78 29
BbeI G_GCGC'C
Cuts at: 1453 4297 4545 1453
Size: 2844 248 3771
Fragments arranged by size:
3771 2844 248
BbsI GAAGACnn'nnnn_
Cuts at: 220 1355 2453 3233 3617 220
Size: 1135 1098 780 384 3466
Fragments arranged by size:
3466 1135 1098 780 384
BbvCI CC'TCA_GC
Cuts at: 1417 2134 1417
Size: 717 6146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1783 1817 3094 3128 1783
Size: 34 1277 34 5518
Fragments arranged by size:
5518 1277 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1642 4761 5817 6863 1642
Size: 3119 1056 1046 1642
Fragments arranged by size:
3119 1642 1056 1046
BclI T'GATC_A
Cuts at: 2801 4383 2801
Size: 1582 5281
BglI GCCn_nnn'nGGC
Cuts at: 274 847 912 1522 2932 3268 274
Size: 573 65 610 1410 336 3869
Fragments arranged by size:
3869 1410 610 573 336 65
BglII A'GATC_T
Cuts at: 2988 4378 6415 2988
Size: 1390 2037 3436
Fragments arranged by size:
3436 2037 1390
BlpI GC'TnA_GC
Cuts at: 115 527 1715 2359 3975 115
Size: 412 1188 644 1616 3003
Fragments arranged by size:
3003 1616 1188 644 412
Bme1580I G_kGCm'C
Cuts at: 241 270 617 1617 4488 4581 5705 241
Size: 29 347 1000 2871 93 1124 1399
Fragments arranged by size:
2871 1399 1124 1000 347 93 29
BmgBI CAC'GTC
Cuts at: 249 249
Size: 6863
BmrI ACTGGGnnnn_n'
Cuts at: 375 2625 4075 4156 4491 6635 375
Size: 2250 1450 81 335 2144 603
Fragments arranged by size:
2250 2144 1450 603 335 81
BmtI G_CTAG'C
Cuts at: 2609 2609
Size: 6863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2976 5230 2345
Size: 631 2254 3978
Fragments arranged by size:
3978 2254 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 376 742 904 1164 1273 2082 2587 2689
Size: 130 366 162 260 109 809 505 102
Cuts at: 2689 2950 3866 246
Size: 261 916 3243
Fragments arranged by size:
3243 916 809 505 366 261 260 162
130 109 102
Bpu10I CC'TnA_GC
Cuts at: 1417 2134 1417
Size: 717 6146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2851 3002 3766 4039 4918 5632 5930 2851
Size: 151 764 273 879 714 298 3784
Fragments arranged by size:
3784 879 764 714 298 273 151
BsaI GGTCTCn'nnnn_
Cuts at: 808 938 1766 3416 6567 808
Size: 130 828 1650 3151 1104
Fragments arranged by size:
3151 1650 1104 828 130
BsaAI yAC'GTr
Cuts at: 4216 4216
Size: 6863
BsaBI GATnn'nnATC
Cuts at: 6 1078 2993 4377 6414 6
Size: 1072 1915 1384 2037 455
Fragments arranged by size:
2037 1915 1384 1072 455
BsaHI Gr'CG_yC
Cuts at: 192 953 1042 1450 2231 4294 4542 5244 6740
Size: 761 89 408 781 2063 248 702 1496
Cuts at: 6740 192
Size: 315
Fragments arranged by size:
2063 1496 781 761 702 408 315 248
89
BsaWI w'CCGG_w
Cuts at: 2678 3093 4052 4573 5251 5662 5809 6083 2678
Size: 415 959 521 678 411 147 274 3458
Fragments arranged by size:
3458 959 678 521 415 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2000 2030 3075 3105 3799 3829 2000
Size: 30 1045 30 694 30 5034
Fragments arranged by size:
5034 1045 694 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 1129 1364 1558 1654 1774 1948 1951 2321
Size: 990 235 194 96 120 174 3 370
Cuts at: 2321 2529 2952 2966 3076 3083 3088 3367 139
Size: 208 423 14 110 7 5 279 3635
Fragments arranged by size:
3635 990 423 370 279 235 208 194
174 120 110 96 14 7 5 3
BseYI C'CCAG_C
Cuts at: 672 1192 1368 1404 1818 2250 2286 2653 2895
Size: 520 176 36 414 432 36 367 242
Cuts at: 2895 3411 3527 4288 5046 5711 6119 672
Size: 516 116 761 758 665 408 1416
Fragments arranged by size:
1416 761 758 665 520 516 432 414
408 367 242 176 116 36 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 718 1014 1042 1288 1762 2643 277
Size: 441 296 28 246 474 881 4497
Fragments arranged by size:
4497 881 474 441 296 246 28
BsiEI CG_ry'CG
Cuts at: 104 2643 4405 4451 5681 104
Size: 2539 1762 46 1230 1286
Fragments arranged by size:
2539 1762 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 785 1799 1916 2042 2405 2876 3877 4655 4845
Size: 1014 117 126 363 471 1001 778 190
Cuts at: 4845 5705 785
Size: 860 1943
Fragments arranged by size:
1943 1014 1001 860 778 471 363 190
126 117
BsmBI CGTCTCn'nnnn_
Cuts at: 491 3142 491
Size: 2651 4212
Bsp1286I G_dGCh'C
Cuts at: 121 241 270 617 785 1617 1799 1916 2013
Size: 120 29 347 168 832 182 117 97
Cuts at: 2013 2042 2405 2876 2908 3520 3598 3877 4488
Size: 29 363 471 32 612 78 279 611
Cuts at: 4488 4581 4655 4845 4907 5705 121
Size: 93 74 190 62 798 1279
Fragments arranged by size:
1279 832 798 612 611 471 363 347
279 190 182 168 120 117 97 93
78 74 62 32 29 29
BspEI T'CCGG_A
Cuts at: 3093 3093
Size: 6863
BspHI T'CATG_A
Cuts at: 5295 5295
Size: 6863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 1784 2199 3913 4127 4429 4810 491
Size: 1293 415 1714 214 302 381 2544
Fragments arranged by size:
2544 1714 1293 415 381 302 214
BsrBI CCG'CTC
Cuts at: 50 162 1008 1784 2834 5155 5209 6693 6858
Size: 112 846 776 1050 2321 54 1484 165
Cuts at: 6858 50
Size: 55
Fragments arranged by size:
2321 1484 1050 846 776 165 112 55
54
BsrDI GCAATG_nn'
Cuts at: 1898 3610 4775 1898
Size: 1712 1165 3986
Fragments arranged by size:
3986 1712 1165
BsrFI r'CCGG_y
Cuts at: 2638 4861 5042 2638
Size: 2223 181 4459
Fragments arranged by size:
4459 2223 181
BssHII G'CGCG_C
Cuts at: 3664 3664
Size: 6863
BssSI C'ACGA_G
Cuts at: 232 691 2043 5134 5842 232
Size: 459 1352 3091 708 1253
Fragments arranged by size:
3091 1352 1253 708 459
BstAPI GCAn_nnn'nTGC
Cuts at: 4240 4240
Size: 6863
BstBI TT'CG_AA
Cuts at: 2033 3114 5224 2033
Size: 1081 2110 3672
Fragments arranged by size:
3672 2110 1081
BstEII G'GTnAC_C
Cuts at: 1152 1152
Size: 6863
BstXI CCAn_nnnn'nTGG
Cuts at: 1344 2004 2601 1344
Size: 660 597 5606
Fragments arranged by size:
5606 660 597
BstYI r'GATC_y
Cuts at: 1 1274 2988 2994 3159 3713 3888 4355 4378
Size: 1273 1714 6 165 554 175 467 23
Cuts at: 4378 4713 4959 5265 5277 5363 6415 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1714 1273 1052 554 467 449 335 306
246 175 165 86 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 560 1144 1376 2437 560
Size: 584 232 1061 4986
Fragments arranged by size:
4986 1061 584 232
BtgI C'CryG_G
Cuts at: 201 1499 2159 2844 3233 3266 201
Size: 1298 660 685 389 33 3798
Fragments arranged by size:
3798 1298 685 660 389 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 447 2384 3243 4813 4994 5157 447
Size: 1937 859 1570 181 163 2153
Fragments arranged by size:
2153 1937 1570 859 181 163
BtsI GCAGTG_nn'
Cuts at: 706 1935 4106 706
Size: 1229 2171 3463
Fragments arranged by size:
3463 2171 1229
DraI TTT'AAA
Cuts at: 3862 3862
Size: 6863
DraIII CAC_nnn'GTG
Cuts at: 1037 1037
Size: 6863
DrdI GACnn_nn'nnGTC
Cuts at: 313 4569 5913 313
Size: 4256 1344 1263
Fragments arranged by size:
4256 1344 1263
EaeI y'GGCC_r
Cuts at: 137 839 848 913 1017 1068 1751 2219 2507
Size: 702 9 65 104 51 683 468 288
Cuts at: 2507 2636 2640 3269 4448 4622 5013 5040 6385
Size: 129 4 629 1179 174 391 27 1345
Cuts at: 6385 137
Size: 615
Fragments arranged by size:
1345 1179 702 683 629 615 468 391
288 174 129 104 65 51 27 9
4
EagI C'GGCC_G
Cuts at: 2640 4448 2640
Size: 1808 5055
EarI CTCTTCn'nnn_
Cuts at: 464 748 790 1486 1672 2030 2134 2555 3032
Size: 284 42 696 186 358 104 421 477
Cuts at: 3032 3757 4886 5096 464
Size: 725 1129 210 2231
Fragments arranged by size:
2231 1129 725 696 477 421 358 284
210 186 104 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3458 5071 5813 5959 6673 3458
Size: 1613 742 146 714 3648
Fragments arranged by size:
3648 1613 742 714 146
EcoICRI GAG'CTC
Cuts at: 783 2040 2403 3875 783
Size: 1257 363 1472 3771
Fragments arranged by size:
3771 1472 1257 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 376 553 742 904 958 1164 1273 1495
Size: 130 177 189 162 54 206 109 222
Cuts at: 1495 1645 2082 2587 2689 2950 3388 3866 4688
Size: 150 437 505 102 261 438 478 822
Cuts at: 4688 5120 5473 6135 246
Size: 432 353 662 974
Fragments arranged by size:
974 822 662 505 478 438 437 432
353 261 222 206 189 177 162 150
130 109 102 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1147 1147
Size: 6863
EcoO109I rG'GnC_Cy
Cuts at: 504 894 1467 1506 1676 2082 2571 2649 2850
Size: 390 573 39 170 406 489 78 201
Cuts at: 2850 3243 4002 504
Size: 393 759 3365
Fragments arranged by size:
3365 759 573 489 406 393 390 201
170 78 39
EcoRI G'AATT_C
Cuts at: 3867 3867
Size: 6863
EcoRV GAT'ATC
Cuts at: 426 426
Size: 6863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4882 4882
Size: 6863
FseI GG_CCGG'CC
Cuts at: 2642 2642
Size: 6863
FspI TGC'GCA
Cuts at: 1442 4644 1442
Size: 3202 3661
HaeII r_GCGC'y
Cuts at: 1453 2159 4297 4545 5775 6546 1453
Size: 706 2138 248 1230 771 1770
Fragments arranged by size:
2138 1770 1230 771 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1680 1712 2947 2979 3075 3107 3144 3176 3547
Size: 32 1235 32 96 32 37 32 371
Cuts at: 3547 3579 3770 3802 4705 4735 4737 4767 1680
Size: 32 191 32 903 30 2 30 3776
Fragments arranged by size:
3776 1235 903 371 191 96 37 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 645 2172 2313 3684 3902 645
Size: 1527 141 1371 218 3606
Fragments arranged by size:
3606 1527 1371 218 141
KasI G'GCGC_C
Cuts at: 1449 4293 4541 1449
Size: 2844 248 3771
Fragments arranged by size:
3771 2844 248
KpnI G_GTAC'C
Cuts at: 3883 3883
Size: 6863
MluI A'CGCG_T
Cuts at: 715 6296 715
Size: 5581 1282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1367 5622 5806 1109
Size: 258 4255 184 2166
Fragments arranged by size:
4255 2166 258 184
MscI TGG'CCA
Cuts at: 139 841 850 1070 1753 3271 4624 139
Size: 702 9 220 683 1518 1353 2378
Fragments arranged by size:
2378 1518 1353 702 683 220 9
MslI CAynn'nnrTG
Cuts at: 760 1999 2197 2281 2348 3565 4852 4979 760
Size: 1239 198 84 67 1217 1287 127 2644
Fragments arranged by size:
2644 1287 1239 1217 198 127 84 67
MspA1I CmG'CkG
Cuts at: 861 2247 3415 3503 3531 3974 4288 4648 5432
Size: 1386 1168 88 28 443 314 360 784
Cuts at: 5432 5677 861
Size: 245 2047
Fragments arranged by size:
2047 1386 1168 784 443 360 314 245
88 28
NaeI GCC'GGC
Cuts at: 2640 5044 2640
Size: 2404 4459
NarI GG'CG_CC
Cuts at: 1450 4294 4542 1450
Size: 2844 248 3771
Fragments arranged by size:
3771 2844 248
NcoI C'CATG_G
Cuts at: 1499 3233 1499
Size: 1734 5129
NgoMIV G'CCGG_C
Cuts at: 2638 5042 2638
Size: 2404 4459
NheI G'CTAG_C
Cuts at: 2605 2605
Size: 6863
NspI r_CATG'y
Cuts at: 1593 1977 2769 3219 3564 3916 4416 6019 1593
Size: 384 792 450 345 352 500 1603 2437
Fragments arranged by size:
2437 1603 792 500 450 384 352 345
PasI CC'CwG_GG
Cuts at: 492 998 1464 2726 3008 3335 492
Size: 506 466 1262 282 327 4020
Fragments arranged by size:
4020 1262 506 466 327 282
PciI A'CATG_T
Cuts at: 3215 3560 6015 3215
Size: 345 2455 4063
Fragments arranged by size:
4063 2455 345
PflMI CCAn_nnn'nTGG
Cuts at: 2496 3486 2496
Size: 990 5873
PfoI T'CCnGG_A
Cuts at: 881 2225 2699 2777 881
Size: 1344 474 78 4967
Fragments arranged by size:
4967 1344 474 78
PmeI GTTT'AAAC
Cuts at: 3862 3862
Size: 6863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3831 5309 5341 3799
Size: 32 1478 32 5321
Fragments arranged by size:
5321 1478 32 32
PpuMI rG'GwC_Cy
Cuts at: 504 1506 2082 2571 2649 2850 3243 504
Size: 1002 576 489 78 201 393 4124
Fragments arranged by size:
4124 1002 576 489 393 201 78
PshAI GACnn'nnGTC
Cuts at: 1159 1159
Size: 6863
PstI C_TGCA'G
Cuts at: 188 488 595 2210 2260 2385 3420 3655 3910
Size: 300 107 1615 50 125 1035 235 255
Cuts at: 3910 4595 188
Size: 685 2456
Fragments arranged by size:
2456 1615 1035 685 300 255 235 125
107 50
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6863
PvuII CAG'CTG
Cuts at: 861 2247 3415 3531 4288 4648 861
Size: 1386 1168 116 757 360 3076
Fragments arranged by size:
3076 1386 1168 757 360 116
RsrII CG'GwC_CG
Cuts at: 5058 5058
Size: 6863
SacI G_AGCT'C
Cuts at: 785 2042 2405 3877 785
Size: 1257 363 1472 3771
Fragments arranged by size:
3771 1472 1257 363
SalI G'TCGA_C
Cuts at: 2170 3682 3900 2170
Size: 1512 218 5133
Fragments arranged by size:
5133 1512 218
SanDI GG'GwC_CC
Cuts at: 2649 2649
Size: 6863
SapI GCTCTTCn'nnn_
Cuts at: 748 790 1486 2030 2555 3032 4886 5096 748
Size: 42 696 544 525 477 1854 210 2515
Fragments arranged by size:
2515 1854 696 544 525 477 210 42
SbfI CC_TGCA'GG
Cuts at: 2210 3910 2210
Size: 1700 5163
ScaI AGT'ACT
Cuts at: 2614 6380 2614
Size: 3766 3097
SfcI C'TryA_G
Cuts at: 33 184 484 591 2206 2256 2381 3416 3651
Size: 151 300 107 1615 50 125 1035 235
Cuts at: 3651 3906 4591 5559 5750 33
Size: 255 685 968 191 1146
Fragments arranged by size:
1615 1146 1035 968 685 300 255 235
191 151 125 107 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 847 912 847
Size: 65 6798
SfoI GGC'GCC
Cuts at: 1451 4295 4543 1451
Size: 2844 248 3771
Fragments arranged by size:
3771 2844 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6863
SmaI CCC'GGG
Cuts at: 510 1704 3885 510
Size: 1194 2181 3488
Fragments arranged by size:
3488 2181 1194
SmlI C'TyrA_G
Cuts at: 2866 3017 3781 4018 4933 5647 5909 2866
Size: 151 764 237 915 714 262 3820
Fragments arranged by size:
3820 915 764 714 262 237 151
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6863
SphI G_CATG'C
Cuts at: 1977 3916 4416 1977
Size: 1939 500 4424
Fragments arranged by size:
4424 1939 500
StuI AGG'CCT
Cuts at: 1942 3649 1942
Size: 1707 5156
StyI C'CwwG_G
Cuts at: 901 1499 1509 1517 1526 2022 2495 3125 3233
Size: 598 10 8 9 496 473 630 108
Cuts at: 3233 3997 4351 901
Size: 764 354 3413
Fragments arranged by size:
3413 764 630 598 496 473 354 108
10 9 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 693 4446 4579 5038 5247 141
Size: 552 3753 133 459 209 1757
Fragments arranged by size:
3753 1757 552 459 209 133
TatI w'GTAC_w
Cuts at: 1742 2612 6378 1742
Size: 870 3766 2227
Fragments arranged by size:
3766 2227 870
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1323 1355 2000 2032 6380 6412 1323
Size: 32 645 32 4348 32 1774
Fragments arranged by size:
4348 1774 645 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 399 3735 4660 399
Size: 3336 925 2602
Fragments arranged by size:
3336 2602 925
XbaI T'CTAG_A
Cuts at: 3894 3894
Size: 6863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 2283 2517 2562 3264 3841 4358 270
Size: 2013 234 45 702 577 517 2775
Fragments arranged by size:
2775 2013 702 577 517 234 45
XmaI C'CCGG_G
Cuts at: 508 1702 3883 508
Size: 1194 2181 3488
Fragments arranged by size:
3488 2181 1194
XmnI GAAnn'nnTTC
Cuts at: 1480 3307 1480
Size: 1827 5036
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaI AscI BaeI BfrBI
BsiWI BsmI BsrGI BstZ17I ClaI CspCI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PmlI
PsiI PspOMI PspXI PsrI SacII SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI