(Circular) (Six-base) MAPSORT of: pF1KA0678.seq Check: 863 from: 1 to: 9842 pF1KA0678 9842 bp With 182 enzymes: SgfI * March 2, 2010 14:24 .. AccI GT'mk_AC Cuts at: 618 2010 3606 5141 6803 6880 618 Size: 1392 1596 1535 1662 77 3580 Fragments arranged by size: 3580 1662 1596 1535 1392 77 Acc65I G'GTAC_C Cuts at: 6858 6858 Size: 9842 AclI AA'CG_TT Cuts at: 9682 9682 Size: 9842 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1769 3151 3647 4065 4310 4439 4910 5864 6020 Size: 1382 496 418 245 129 471 954 156 Cuts at: 6020 6195 6641 7667 8099 8452 9114 1769 Size: 175 446 1026 432 353 662 2497 Fragments arranged by size: 2497 1382 1026 954 662 496 471 446 432 418 353 245 175 156 129 AflII C'TTAA_G Cuts at: 1578 3184 1578 Size: 1606 8236 AflIII A'CryG_T Cuts at: 886 3254 4130 4511 7194 8994 9275 886 Size: 2368 876 381 2683 1800 281 1453 Fragments arranged by size: 2683 2368 1800 1453 876 381 281 AhdI GACnn_n'nnGTC Cuts at: 3214 3214 Size: 9842 AleI CACnn'nnGTG Cuts at: 470 470 Size: 9842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 668 1931 668 Size: 1263 8579 AlwNI CAG_nnn'CTG Cuts at: 1225 3134 3378 5404 6421 6736 7147 8585 1225 Size: 1909 244 2026 1017 315 411 1438 2482 Fragments arranged by size: 2482 2026 1909 1438 1017 411 315 244 ApaLI G'TGCA_C Cuts at: 2817 8680 2817 Size: 5863 3979 ApoI r'AATT_y Cuts at: 358 748 997 1648 2185 2245 2436 2662 3691 Size: 390 249 651 537 60 191 226 1029 Cuts at: 3691 4159 4733 5068 5134 5800 6109 6846 9195 Size: 468 574 335 66 666 309 737 2349 Cuts at: 9195 9451 358 Size: 256 749 Fragments arranged by size: 2349 1029 749 737 666 651 574 537 468 390 335 309 256 249 226 191 66 60 AseI AT'TA_AT Cuts at: 20 527 6446 20 Size: 507 5919 3416 Fragments arranged by size: 5919 3416 507 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9842 AvaI C'yCGr_G Cuts at: 6862 6862 Size: 9842 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4081 4114 4524 4557 4081 Size: 33 410 33 9366 Fragments arranged by size: 9366 410 33 33 BamHI G'GATC_C Cuts at: 6867 6867 Size: 9842 BanI G'GyrC_C Cuts at: 1678 1930 3731 6858 7272 7520 7555 1678 Size: 252 1801 3127 414 248 35 3965 Fragments arranged by size: 3965 3127 1801 414 252 248 35 BanII G_rGCy'C Cuts at: 3141 4427 5727 6146 6856 7886 3141 Size: 1286 1300 419 710 1030 5097 Fragments arranged by size: 5097 1300 1286 1030 710 419 BbeI G_GCGC'C Cuts at: 3735 7276 7524 3735 Size: 3541 248 6053 Fragments arranged by size: 6053 3541 248 BbsI GAAGACnn'nnnn_ Cuts at: 2358 3775 6048 2358 Size: 1417 2273 6152 Fragments arranged by size: 6152 2273 1417 BbvCI CC'TCA_GC Cuts at: 6224 6224 Size: 9842 BciVI GTATCCnnnnn_n' Cuts at: 4282 4525 6715 7740 8796 9842 4282 Size: 243 2190 1025 1056 1046 4282 Fragments arranged by size: 4282 2190 1056 1046 1025 243 BclI T'GATC_A Cuts at: 1316 3260 5148 7362 1316 Size: 1944 1888 2214 3796 Fragments arranged by size: 3796 2214 1944 1888 BfrBI ATG'CAT Cuts at: 1563 2142 5417 1563 Size: 579 3275 5988 Fragments arranged by size: 5988 3275 579 BglI GCCn_nnn'nGGC Cuts at: 6574 6574 Size: 9842 BglII A'GATC_T Cuts at: 588 2109 7357 9394 588 Size: 1521 5248 2037 1036 Fragments arranged by size: 5248 2037 1521 1036 BlpI GC'TnA_GC Cuts at: 4932 6954 4932 Size: 2022 7820 Bme1580I G_kGCm'C Cuts at: 1548 1683 2821 3617 5426 7467 7560 8684 1548 Size: 135 1138 796 1809 2041 93 1124 2706 Fragments arranged by size: 2706 2041 1809 1138 1124 796 135 93 BmrI ACTGGGnnnn_n' Cuts at: 7054 7135 7470 9614 7054 Size: 81 335 2144 7282 Fragments arranged by size: 7282 2144 335 81 BmtI G_CTAG'C Cuts at: 4384 4384 Size: 9842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8209 8209 Size: 9842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 489 491 1663 2304 2987 3686 3735 4889 5239 Size: 2 1172 641 683 699 49 1154 350 Cuts at: 5239 6684 6758 489 Size: 1445 74 3573 Fragments arranged by size: 3573 1445 1172 1154 699 683 641 350 74 49 2 Bpu10I CC'TnA_GC Cuts at: 6224 6224 Size: 9842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 462 1402 3125 4019 6081 6580 7018 7897 8611 Size: 940 1723 894 2062 499 438 879 714 Cuts at: 8611 8909 462 Size: 298 1395 Fragments arranged by size: 2062 1723 1395 940 894 879 714 499 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 1294 6370 9546 1294 Size: 5076 3176 1590 Fragments arranged by size: 5076 3176 1590 BsaAI yAC'GTr Cuts at: 7195 7195 Size: 9842 BsaBI GATnn'nnATC Cuts at: 6 2132 4112 7356 9393 6 Size: 2126 1980 3244 2037 455 Fragments arranged by size: 3244 2126 2037 1980 455 BsaHI Gr'CG_yC Cuts at: 3732 7273 7521 8223 9719 3732 Size: 3541 248 702 1496 3855 Fragments arranged by size: 3855 3541 1496 702 248 BsaWI w'CCGG_w Cuts at: 3902 7031 7552 8230 8641 8788 9062 3902 Size: 3129 521 678 411 147 274 4682 Fragments arranged by size: 4682 3129 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 6617 6647 6617 Size: 30 9812 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1419 6065 1419 Size: 4646 5196 BseYI C'CCAG_C Cuts at: 1849 3134 3179 6561 7267 8025 8690 9098 1849 Size: 1285 45 3382 706 758 665 408 2593 Fragments arranged by size: 3382 2593 1285 758 706 665 408 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 5494 5494 Size: 9842 BsiEI CG_ry'CG Cuts at: 104 7384 7430 8660 104 Size: 7280 46 1230 1286 Fragments arranged by size: 7280 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 374 1731 2821 3804 4427 5954 6146 6856 7634 Size: 1357 1090 983 623 1527 192 710 778 Cuts at: 7634 7824 8684 374 Size: 190 860 1532 Fragments arranged by size: 1532 1527 1357 1090 983 860 778 710 623 192 190 BsmI GAATG_Cn' Cuts at: 4579 4793 5419 4579 Size: 214 626 9002 Fragments arranged by size: 9002 626 214 Bsp1286I G_dGCh'C Cuts at: 374 1548 1683 1731 2821 3141 3617 3804 4427 Size: 1174 135 48 1090 320 476 187 623 Cuts at: 4427 5426 5727 5954 6146 6856 7467 7560 7634 Size: 999 301 227 192 710 611 93 74 Cuts at: 7634 7824 7886 8684 374 Size: 190 62 798 1532 Fragments arranged by size: 1532 1174 1090 999 798 710 623 611 476 320 301 227 192 190 187 135 93 74 62 48 BspHI T'CATG_A Cuts at: 3081 3491 5874 8274 3081 Size: 410 2383 2400 4649 Fragments arranged by size: 4649 2400 2383 410 BspMI ACCTGCnnnn'nnnn_ Cuts at: 6751 6797 6892 7106 7408 7789 6751 Size: 46 95 214 302 381 8804 Fragments arranged by size: 8804 381 302 214 95 46 BsrBI CCG'CTC Cuts at: 50 8134 8188 9672 9837 50 Size: 8084 54 1484 165 55 Fragments arranged by size: 8084 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 721 2174 2300 2991 3638 5581 5663 6284 6608 Size: 1453 126 691 647 1943 82 621 324 Cuts at: 6608 7754 721 Size: 1146 2809 Fragments arranged by size: 2809 1943 1453 1146 691 647 621 324 126 82 BsrFI r'CCGG_y Cuts at: 7840 8021 7840 Size: 181 9661 BsrGI T'GTAC_A Cuts at: 3497 5853 3497 Size: 2356 7486 BssSI C'ACGA_G Cuts at: 2447 8113 8821 2447 Size: 5666 708 3468 Fragments arranged by size: 5666 3468 708 BstAPI GCAn_nnn'nTGC Cuts at: 5239 5668 7219 5239 Size: 429 1551 7862 Fragments arranged by size: 7862 1551 429 BstBI TT'CG_AA Cuts at: 2343 5784 8203 2343 Size: 3441 2419 3982 Fragments arranged by size: 3982 3441 2419 BstXI CCAn_nnnn'nTGG Cuts at: 5565 5565 Size: 9842 BstYI r'GATC_y Cuts at: 1 132 588 2109 2265 2382 2892 4200 4614 Size: 131 456 1521 156 117 510 1308 414 Cuts at: 4614 6065 6867 7334 7357 7692 7938 8244 8256 Size: 1451 802 467 23 335 246 306 12 Cuts at: 8256 8342 9394 1 Size: 86 1052 449 Fragments arranged by size: 1521 1451 1308 1052 802 510 467 456 449 414 335 306 246 156 131 117 86 23 12 BstZ17I GTA'TAC Cuts at: 619 3607 619 Size: 2988 6854 Bsu36I CC'TnA_GG Cuts at: 1183 5978 1183 Size: 4795 5047 BtgI C'CryG_G Cuts at: 1829 6362 1829 Size: 4533 5309 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1146 1972 2668 7792 7973 8136 1146 Size: 826 696 5124 181 163 2852 Fragments arranged by size: 5124 2852 826 696 181 163 BtsI GCAGTG_nn' Cuts at: 339 2974 3726 7085 339 Size: 2635 752 3359 3096 Fragments arranged by size: 3359 3096 2635 752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 3079 3114 4169 4204 3079 Size: 35 1055 35 8717 Fragments arranged by size: 8717 1055 35 35 DraI TTT'AAA Cuts at: 356 1809 5958 6841 356 Size: 1453 4149 883 3357 Fragments arranged by size: 4149 3357 1453 883 DraIII CAC_nnn'GTG Cuts at: 3269 3269 Size: 9842 DrdI GACnn_nn'nnGTC Cuts at: 7548 8892 7548 Size: 1344 8498 EaeI y'GGCC_r Cuts at: 3916 5294 7427 7601 7992 8019 9364 3916 Size: 1378 2133 174 391 27 1345 4394 Fragments arranged by size: 4394 2133 1378 1345 391 174 27 EagI C'GGCC_G Cuts at: 7427 7427 Size: 9842 EarI CTCTTCn'nnn_ Cuts at: 665 1297 1613 2392 2857 3566 4249 4415 4425 Size: 632 316 779 465 709 683 166 10 Cuts at: 4425 4778 5450 5576 6214 7865 8075 665 Size: 353 672 126 638 1651 210 2432 Fragments arranged by size: 2432 1651 779 709 683 672 638 632 465 353 316 210 166 126 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 8050 8792 8938 9652 8050 Size: 742 146 714 8240 Fragments arranged by size: 8240 742 714 146 EcoICRI GAG'CTC Cuts at: 4425 6144 6854 4425 Size: 1719 710 7413 Fragments arranged by size: 7413 1719 710 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 489 491 1663 1769 2304 2987 3151 3647 3686 Size: 2 1172 106 535 683 164 496 39 Cuts at: 3686 3735 4065 4310 4439 4889 4910 5239 5864 Size: 49 330 245 129 450 21 329 625 Cuts at: 5864 6020 6195 6641 6684 6758 7667 8099 8452 Size: 156 175 446 43 74 909 432 353 Cuts at: 8452 9114 489 Size: 662 1217 Fragments arranged by size: 1217 1172 909 683 662 625 535 496 450 446 432 353 330 329 245 175 164 156 129 106 74 49 43 39 21 2 EcoNI CCTnn'n_nnAGG Cuts at: 2572 4723 2572 Size: 2151 7691 EcoO109I rG'GnC_Cy Cuts at: 2773 6240 6733 6981 2773 Size: 3467 493 248 5634 Fragments arranged by size: 5634 3467 493 248 EcoRI G'AATT_C Cuts at: 748 6846 748 Size: 6098 3744 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 204 1624 2284 2485 3970 7861 204 Size: 1420 660 201 1485 3891 2185 Fragments arranged by size: 3891 2185 1485 1420 660 201 FspI TGC'GCA Cuts at: 7623 7623 Size: 9842 HaeII r_GCGC'y Cuts at: 3735 7276 7524 8754 9525 3735 Size: 3541 248 1230 771 4052 Fragments arranged by size: 4052 3541 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 3200 3232 4571 4603 5427 5459 5823 5855 5905 Size: 32 1339 32 824 32 364 32 50 Cuts at: 5905 5937 7684 7714 7716 7746 3200 Size: 32 1747 30 2 30 5296 Fragments arranged by size: 5296 1747 1339 824 364 50 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 4398 5529 6881 4398 Size: 1131 1352 7359 Fragments arranged by size: 7359 1352 1131 HindIII A'AGCT_T Cuts at: 1601 3937 4073 4929 1601 Size: 2336 136 856 6514 Fragments arranged by size: 6514 2336 856 136 KasI G'GCGC_C Cuts at: 3731 7272 7520 3731 Size: 3541 248 6053 Fragments arranged by size: 6053 3541 248 KpnI G_GTAC'C Cuts at: 6862 6862 Size: 9842 MluI A'CGCG_T Cuts at: 9275 9275 Size: 9842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 177 267 4939 5238 6027 8601 8785 177 Size: 90 4672 299 789 2574 184 1234 Fragments arranged by size: 4672 2574 1234 789 299 184 90 MscI TGG'CCA Cuts at: 3918 5296 7603 3918 Size: 1378 2307 6157 Fragments arranged by size: 6157 2307 1378 MslI CAynn'nnrTG Cuts at: 470 2803 2951 4594 5290 7831 7958 470 Size: 2333 148 1643 696 2541 127 2354 Fragments arranged by size: 2541 2354 2333 1643 696 148 127 MspA1I CmG'CkG Cuts at: 2035 6228 6953 7267 7627 8411 8656 2035 Size: 4193 725 314 360 784 245 3221 Fragments arranged by size: 4193 3221 784 725 360 314 245 NaeI GCC'GGC Cuts at: 8023 8023 Size: 9842 NarI GG'CG_CC Cuts at: 3732 7273 7521 3732 Size: 3541 248 6053 Fragments arranged by size: 6053 3541 248 NcoI C'CATG_G Cuts at: 1829 6362 1829 Size: 4533 5309 NdeI CA'TA_TG Cuts at: 4155 4155 Size: 9842 NgoMIV G'CCGG_C Cuts at: 8021 8021 Size: 9842 NheI G'CTAG_C Cuts at: 4380 4380 Size: 9842 NruI TCG'CGA Cuts at: 1463 1463 Size: 9842 NsiI A_TGCA'T Cuts at: 1565 2144 5419 1565 Size: 579 3275 5988 Fragments arranged by size: 5988 3275 579 NspI r_CATG'y Cuts at: 143 890 1152 1273 2146 3258 4134 4515 4944 Size: 747 262 121 873 1112 876 381 429 Cuts at: 4944 6895 7395 8998 143 Size: 1951 500 1603 987 Fragments arranged by size: 1951 1603 1112 987 876 873 747 500 429 381 262 121 PciI A'CATG_T Cuts at: 886 3254 4130 4511 8994 886 Size: 2368 876 381 4483 1734 Fragments arranged by size: 4483 2368 1734 876 381 PflMI CCAn_nnn'nTGG Cuts at: 3134 3134 Size: 9842 PfoI T'CCnGG_A Cuts at: 507 2530 507 Size: 2023 7819 PmeI GTTT'AAAC Cuts at: 6841 6841 Size: 9842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1375 1407 8288 8320 1375 Size: 32 6881 32 2897 Fragments arranged by size: 6881 2897 32 32 PpuMI rG'GwC_Cy Cuts at: 2773 6240 6733 2773 Size: 3467 493 5882 Fragments arranged by size: 5882 3467 493 PshAI GACnn'nnGTC Cuts at: 3339 3339 Size: 9842 PsiI TTA'TAA Cuts at: 1003 4277 1003 Size: 3274 6568 PstI C_TGCA'G Cuts at: 6889 7574 6889 Size: 685 9157 PvuI CG_AT'CG Cuts at: 104 104 Size: 9842 PvuII CAG'CTG Cuts at: 2035 6228 7267 7627 2035 Size: 4193 1039 360 4250 Fragments arranged by size: 4250 4193 1039 360 RsrII CG'GwC_CG Cuts at: 8037 8037 Size: 9842 SacI G_AGCT'C Cuts at: 4427 6146 6856 4427 Size: 1719 710 7413 Fragments arranged by size: 7413 1719 710 SalI G'TCGA_C Cuts at: 6879 6879 Size: 9842 SapI GCTCTTCn'nnn_ Cuts at: 4415 5576 7865 8075 4415 Size: 1161 2289 210 6182 Fragments arranged by size: 6182 2289 1161 210 SbfI CC_TGCA'GG Cuts at: 6889 6889 Size: 9842 ScaI AGT'ACT Cuts at: 548 9359 548 Size: 8811 1031 SfcI C'TryA_G Cuts at: 33 365 564 2966 4373 4548 4708 5142 6800 Size: 332 199 2402 1407 175 160 434 1658 Cuts at: 6800 6885 7570 8538 8729 33 Size: 85 685 968 191 1146 Fragments arranged by size: 2402 1658 1407 1146 968 685 434 332 199 191 175 160 85 SfoI GGC'GCC Cuts at: 3733 7274 7522 3733 Size: 3541 248 6053 Fragments arranged by size: 6053 3541 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9842 SmaI CCC'GGG Cuts at: 6864 6864 Size: 9842 SmlI C'TyrA_G Cuts at: 477 1381 1578 3140 3184 4034 6060 6595 6997 Size: 904 197 1562 44 850 2026 535 402 Cuts at: 6997 7912 8626 8888 477 Size: 915 714 262 1431 Fragments arranged by size: 2026 1562 1431 915 904 850 714 535 402 262 197 44 SpeI A'CTAG_T Cuts at: 67 67 Size: 9842 SphI G_CATG'C Cuts at: 1152 4944 6895 7395 1152 Size: 3792 1951 500 3599 Fragments arranged by size: 3792 3599 1951 500 SspI AAT'ATT Cuts at: 573 760 921 1024 1248 2496 3870 5535 573 Size: 187 161 103 224 1248 1374 1665 4880 Fragments arranged by size: 4880 1665 1374 1248 224 187 161 103 StyI C'CwwG_G Cuts at: 1829 4749 4872 6362 6822 6976 7330 1829 Size: 2920 123 1490 460 154 354 4341 Fragments arranged by size: 4341 2920 1490 460 354 154 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 7425 7558 8017 8226 7425 Size: 133 459 209 9041 Fragments arranged by size: 9041 459 209 133 TatI w'GTAC_w Cuts at: 546 3497 4518 5635 5853 9357 546 Size: 2951 1021 1117 218 3504 1031 Fragments arranged by size: 3504 2951 1117 1031 1021 218 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1434 1466 3342 3374 4223 4255 4433 4465 5007 Size: 32 1876 32 849 32 178 32 542 Cuts at: 5007 5039 6617 6649 9359 9391 1434 Size: 32 1578 32 2710 32 1885 Fragments arranged by size: 2710 1885 1876 1578 849 542 178 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5524 7639 5524 Size: 2115 7727 XbaI T'CTAG_A Cuts at: 6873 6873 Size: 9842 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1666 2259 3710 5033 7337 1666 Size: 593 1451 1323 2304 4171 Fragments arranged by size: 4171 2304 1451 1323 593 XmaI C'CCGG_G Cuts at: 6862 6862 Size: 9842 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AgeI AloI ApaI AscI AvrII BcgI BmgBI BsiWI BsmBI BspEI BssHII BstEII ClaI EcoRV FseI FspAI HpaI MfeI NotI PacI PasI PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI