Restriction map for
pF1KA0687
(Circular) (Six-base) MAPSORT of: pF1KA0687.seq Check: 9124 from: 1 to: 6224
pF1KA0687 6224 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 234 1156 2450 3262 234
Size: 922 1294 812 3196
Fragments arranged by size:
3196 1294 922 812
Acc65I G'GTAC_C
Cuts at: 3240 3240
Size: 6224
AclI AA'CG_TT
Cuts at: 1203 6064 1203
Size: 4861 1363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1563 1625 1670 4049 4481 4834 5496 886
Size: 677 62 45 2379 432 353 662 1614
Fragments arranged by size:
2379 1614 677 662 432 353 62 45
AflII C'TTAA_G
Cuts at: 1012 1012
Size: 6224
AflIII A'CryG_T
Cuts at: 237 2445 2811 3576 5376 5657 237
Size: 2208 366 765 1800 281 804
Fragments arranged by size:
2208 1800 804 765 366 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3067 3099 3067
Size: 32 6192
AlwNI CAG_nnn'CTG
Cuts at: 1626 3529 4967 1626
Size: 1903 1438 2883
Fragments arranged by size:
2883 1903 1438
ApaLI G'TGCA_C
Cuts at: 5062 5062
Size: 6224
ApoI r'AATT_y
Cuts at: 1884 2289 2581 2647 3112 3228 5577 5833 1884
Size: 405 292 66 465 116 2349 256 2275
Fragments arranged by size:
2349 2275 465 405 292 256 116 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 6224
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
AvaI C'yCGr_G
Cuts at: 1288 1537 3244 1288
Size: 249 1707 4268
Fragments arranged by size:
4268 1707 249
BamHI G'GATC_C
Cuts at: 165 1528 2743 3249 165
Size: 1363 1215 506 3140
Fragments arranged by size:
3140 1363 1215 506
BanI G'GyrC_C
Cuts at: 208 911 1482 2628 3240 3654 3902 3937 208
Size: 703 571 1146 612 414 248 35 2495
Fragments arranged by size:
2495 1146 703 612 571 414 248 35
BanII G_rGCy'C
Cuts at: 388 1295 1451 3102 3238 4268 388
Size: 907 156 1651 136 1030 2344
Fragments arranged by size:
2344 1651 1030 907 156 136
BbeI G_GCGC'C
Cuts at: 3658 3906 3658
Size: 248 5976
BbsI GAAGACnn'nnnn_
Cuts at: 494 2376 494
Size: 1882 4342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 106 140 1965 1999 106
Size: 34 1825 34 4331
Fragments arranged by size:
4331 1825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4122 5178 6224 4122
Size: 1056 1046 4122
Fragments arranged by size:
4122 1056 1046
BclI T'GATC_A
Cuts at: 3744 3744
Size: 6224
BfrBI ATG'CAT
Cuts at: 816 816
Size: 6224
BglII A'GATC_T
Cuts at: 3739 5776 3739
Size: 2037 4187
BlpI GC'TnA_GC
Cuts at: 635 3336 635
Size: 2701 3523
Bme1580I G_kGCm'C
Cuts at: 1487 1978 2631 3849 3942 5066 1487
Size: 491 653 1218 93 1124 2645
Fragments arranged by size:
2645 1218 1124 653 491 93
BmrI ACTGGGnnnn_n'
Cuts at: 3436 3517 3852 5996 3436
Size: 81 335 2144 3664
Fragments arranged by size:
3664 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1863 4591 1863
Size: 2728 3496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 374 492 2002 331
Size: 43 118 1510 4553
Fragments arranged by size:
4553 1510 118 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2416 3400 4279 4993 5291 1663
Size: 753 984 879 714 298 2596
Fragments arranged by size:
2596 984 879 753 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1547 1559 2130 5928 1547
Size: 12 571 3798 1843
Fragments arranged by size:
3798 1843 571 12
BsaAI yAC'GTr
Cuts at: 2446 3577 2446
Size: 1131 5093
BsaBI GATnn'nnATC
Cuts at: 6 1593 3738 5775 6
Size: 1587 2145 2037 455
Fragments arranged by size:
2145 2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 3655 3903 4605 6101 3655
Size: 248 702 1496 3778
Fragments arranged by size:
3778 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3413 3934 4612 5023 5170 5444 3413
Size: 521 678 411 147 274 4193
Fragments arranged by size:
4193 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 2663 2693 139
Size: 30 2494 30 3670
Fragments arranged by size:
3670 2494 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 845 1111 1497 1761 1798 1819 2028 144
Size: 701 266 386 264 37 21 209 4340
Fragments arranged by size:
4340 701 386 266 264 209 37 21
BseYI C'CCAG_C
Cuts at: 172 436 1532 3026 3215 3649 4407 5072 5480
Size: 264 1096 1494 189 434 758 665 408
Cuts at: 5480 172
Size: 916
Fragments arranged by size:
1494 1096 916 758 665 434 408 264
189
BsiEI CG_ry'CG
Cuts at: 104 3766 3812 5042 104
Size: 3662 46 1230 1286
Fragments arranged by size:
3662 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 636 798 831 3238 4016 4206 5066 636
Size: 162 33 2407 778 190 860 1794
Fragments arranged by size:
2407 1794 860 778 190 162 33
BsmI GAATG_Cn'
Cuts at: 603 603
Size: 6224
Bsp1286I G_dGCh'C
Cuts at: 388 636 798 831 1295 1451 1487 1978 2631
Size: 248 162 33 464 156 36 491 653
Cuts at: 2631 3102 3238 3849 3942 4016 4206 4268 5066
Size: 471 136 611 93 74 190 62 798
Cuts at: 5066 388
Size: 1546
Fragments arranged by size:
1546 798 653 611 491 471 464 248
190 162 156 136 93 74 62 36
33
BspHI T'CATG_A
Cuts at: 3185 4656 3185
Size: 1471 4753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3274 3488 3790 4171 3274
Size: 214 302 381 5327
Fragments arranged by size:
5327 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4516 4570 6054 6219 50
Size: 4466 54 1484 165 55
Fragments arranged by size:
4466 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 1899 3135 4136 350
Size: 1549 1236 1001 2438
Fragments arranged by size:
2438 1549 1236 1001
BsrFI r'CCGG_y
Cuts at: 1950 4222 4403 1950
Size: 2272 181 3771
Fragments arranged by size:
3771 2272 181
BsrGI T'GTAC_A
Cuts at: 2547 2547
Size: 6224
BssSI C'ACGA_G
Cuts at: 4495 5203 4495
Size: 708 5516
BstAPI GCAn_nnn'nTGC
Cuts at: 793 3601 793
Size: 2808 3416
BstBI TT'CG_AA
Cuts at: 4585 4585
Size: 6224
BstXI CCAn_nnnn'nTGG
Cuts at: 783 783
Size: 6224
BstYI r'GATC_y
Cuts at: 1 165 1528 1698 2157 2700 2743 3047 3249
Size: 164 1363 170 459 543 43 304 202
Cuts at: 3249 3716 3739 4074 4320 4626 4638 4724 5776
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5776 1
Size: 449
Fragments arranged by size:
1363 1052 543 467 459 449 335 306
304 246 202 170 164 86 43 23
12
Bsu36I CC'TnA_GG
Cuts at: 520 698 1509 1848 3192 520
Size: 178 811 339 1344 3552
Fragments arranged by size:
3552 1344 811 339 178
BtgI C'CryG_G
Cuts at: 107 107
Size: 6224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 690 4174 4355 4518 690
Size: 3484 181 163 2396
Fragments arranged by size:
3484 2396 181 163
BtsI GCAGTG_nn'
Cuts at: 1384 1401 1503 1643 1736 3467 1384
Size: 17 102 140 93 1731 4141
Fragments arranged by size:
4141 1731 140 102 93 17
DraI TTT'AAA
Cuts at: 3223 3223
Size: 6224
DraIII CAC_nnn'GTG
Cuts at: 2215 2215
Size: 6224
DrdI GACnn_nn'nnGTC
Cuts at: 3930 5274 3930
Size: 1344 4880
EaeI y'GGCC_r
Cuts at: 1712 1744 1952 2337 3809 3983 4374 4401 5746
Size: 32 208 385 1472 174 391 27 1345
Cuts at: 5746 1712
Size: 2190
Fragments arranged by size:
2190 1472 1345 391 385 208 174 32
27
EagI C'GGCC_G
Cuts at: 3809 3809
Size: 6224
EarI CTCTTCn'nnn_
Cuts at: 287 1036 1088 1166 1220 2239 2596 3212 4247
Size: 749 52 78 54 1019 357 616 1035
Cuts at: 4247 4457 287
Size: 210 2054
Fragments arranged by size:
2054 1035 1019 749 616 357 210 78
54 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 672 1978 4432 5174 5320 6034 672
Size: 1306 2454 742 146 714 862
Fragments arranged by size:
2454 1306 862 742 714 146
EcoICRI GAG'CTC
Cuts at: 3236 3236
Size: 6224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 374 492 886 1563 1625 1670 2002 4049
Size: 43 118 394 677 62 45 332 2047
Cuts at: 4049 4481 4834 5496 331
Size: 432 353 662 1059
Fragments arranged by size:
2047 1059 677 662 432 394 353 332
118 62 45 43
EcoNI CCTnn'n_nnAGG
Cuts at: 3195 3195
Size: 6224
EcoO109I rG'GnC_Cy
Cuts at: 3363 3363
Size: 6224
EcoRI G'AATT_C
Cuts at: 3228 3228
Size: 6224
EcoRV GAT'ATC
Cuts at: 567 567
Size: 6224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1293 3159 4243 1293
Size: 1866 1084 3274
Fragments arranged by size:
3274 1866 1084
FspI TGC'GCA
Cuts at: 4005 4005
Size: 6224
HaeII r_GCGC'y
Cuts at: 1626 3658 3906 5136 5907 1626
Size: 2032 248 1230 771 1943
Fragments arranged by size:
2032 1943 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 742 774 1756 1788 2767 2799 3037
Size: 32 571 32 982 32 979 32 238
Cuts at: 3037 3069 4066 4096 4098 4128 139
Size: 32 997 30 2 30 2235
Fragments arranged by size:
2235 997 982 979 571 238 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 235 618 3263 235
Size: 383 2645 3196
Fragments arranged by size:
3196 2645 383
HindIII A'AGCT_T
Cuts at: 1275 1275
Size: 6224
KasI G'GCGC_C
Cuts at: 3654 3902 3654
Size: 248 5976
KpnI G_GTAC'C
Cuts at: 3244 3244
Size: 6224
MluI A'CGCG_T
Cuts at: 5657 5657
Size: 6224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 180 4983 5167 180
Size: 4803 184 1237
Fragments arranged by size:
4803 1237 184
MscI TGG'CCA
Cuts at: 1714 2339 3985 1714
Size: 625 1646 3953
Fragments arranged by size:
3953 1646 625
MslI CAynn'nnrTG
Cuts at: 781 1934 3074 4213 4340 781
Size: 1153 1140 1139 127 2665
Fragments arranged by size:
2665 1153 1140 1139 127
MspA1I CmG'CkG
Cuts at: 639 936 3335 3649 4009 4793 5038 639
Size: 297 2399 314 360 784 245 1825
Fragments arranged by size:
2399 1825 784 360 314 297 245
NaeI GCC'GGC
Cuts at: 1952 4405 1952
Size: 2453 3771
NarI GG'CG_CC
Cuts at: 3655 3903 3655
Size: 248 5976
NcoI C'CATG_G
Cuts at: 107 107
Size: 6224
NdeI CA'TA_TG
Cuts at: 2940 2940
Size: 6224
NgoMIV G'CCGG_C
Cuts at: 1950 4403 1950
Size: 2453 3771
NsiI A_TGCA'T
Cuts at: 818 818
Size: 6224
NspI r_CATG'y
Cuts at: 241 816 933 2815 3277 3777 5380 241
Size: 575 117 1882 462 500 1603 1085
Fragments arranged by size:
1882 1603 1085 575 500 462 117
PasI CC'CwG_GG
Cuts at: 1549 1549
Size: 6224
PciI A'CATG_T
Cuts at: 237 2811 5376 237
Size: 2574 2565 1085
Fragments arranged by size:
2574 2565 1085
PfoI T'CCnGG_A
Cuts at: 390 1250 390
Size: 860 5364
PmeI GTTT'AAAC
Cuts at: 3223 3223
Size: 6224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1335 1367 2815 2847 4670 4702 1335
Size: 32 1448 32 1823 32 2857
Fragments arranged by size:
2857 1823 1448 32 32 32
PsiI TTA'TAA
Cuts at: 973 2555 973
Size: 1582 4642
PstI C_TGCA'G
Cuts at: 1187 1391 3271 3956 1187
Size: 204 1880 685 3455
Fragments arranged by size:
3455 1880 685 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6224
PvuII CAG'CTG
Cuts at: 639 3649 4009 639
Size: 3010 360 2854
Fragments arranged by size:
3010 2854 360
RsrII CG'GwC_CG
Cuts at: 4419 4419
Size: 6224
SacI G_AGCT'C
Cuts at: 3238 3238
Size: 6224
SalI G'TCGA_C
Cuts at: 233 3261 233
Size: 3028 3196
SapI GCTCTTCn'nnn_
Cuts at: 1220 4247 4457 1220
Size: 3027 210 2987
Fragments arranged by size:
3027 2987 210
SbfI CC_TGCA'GG
Cuts at: 3271 3271
Size: 6224
ScaI AGT'ACT
Cuts at: 5741 5741
Size: 6224
SfcI C'TryA_G
Cuts at: 33 944 1183 1234 1387 2071 2968 3267 3952
Size: 911 239 51 153 684 897 299 685
Cuts at: 3952 4920 5111 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 911 897 685 684 299 239
191 153 51
SfoI GGC'GCC
Cuts at: 3656 3904 3656
Size: 248 5976
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
SmaI CCC'GGG
Cuts at: 1539 3246 1539
Size: 1707 4517
SmlI C'TyrA_G
Cuts at: 1012 1678 2395 3379 4294 5008 5270 1012
Size: 666 717 984 915 714 262 1966
Fragments arranged by size:
1966 984 915 717 714 666 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6224
SphI G_CATG'C
Cuts at: 3277 3777 3277
Size: 500 5724
StyI C'CwwG_G
Cuts at: 107 1967 2340 2954 3358 3712 107
Size: 1860 373 614 404 354 2619
Fragments arranged by size:
2619 1860 614 404 373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1334 3146 3807 3940 4399 4608 1334
Size: 1812 661 133 459 209 2950
Fragments arranged by size:
2950 1812 661 459 209 133
TatI w'GTAC_w
Cuts at: 1073 2547 5739 1073
Size: 1474 3192 1558
Fragments arranged by size:
3192 1558 1474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2017 2049 2084 2116 5741 5773 2017
Size: 32 35 32 3625 32 2468
Fragments arranged by size:
3625 2468 35 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1998 4021 1998
Size: 2023 4201
XbaI T'CTAG_A
Cuts at: 3255 3255
Size: 6224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 3018 3719 270
Size: 2748 701 2775
Fragments arranged by size:
2775 2748 701
XmaI C'CCGG_G
Cuts at: 1537 3244 1537
Size: 1707 4517
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BspEI BssHII BstEII BstZ17I ClaI
CspCI FseI FspAI HpaI MfeI NheI NotI NruI
PacI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI