Restriction map for
pF1KA0691
(Circular) (Six-base) MAPSORT of: pF1KA0691.seq Check: 6047 from: 1 to: 5372
pF1KA0691 5372 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 510 510
Size: 5372
AccI GT'mk_AC
Cuts at: 2107 2410 2107
Size: 303 5069
Acc65I G'GTAC_C
Cuts at: 2388 2388
Size: 5372
AclI AA'CG_TT
Cuts at: 5212 5212
Size: 5372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 1626 3197 3629 4376 4644 974
Size: 652 1571 432 747 268 1702
Fragments arranged by size:
1702 1571 747 652 432 268
AflIII A'CryG_T
Cuts at: 2724 3828 4805 2724
Size: 1104 977 3291
Fragments arranged by size:
3291 1104 977
AgeI A'CCGG_T
Cuts at: 3760 3760
Size: 5372
AlwNI CAG_nnn'CTG
Cuts at: 819 2103 2163 2677 4244 819
Size: 1284 60 514 1567 1947
Fragments arranged by size:
1947 1567 1284 514 60
ApaI G_GGCC'C
Cuts at: 1171 1256 1477 1918 1171
Size: 85 221 441 4625
Fragments arranged by size:
4625 441 221 85
ApaLI G'TGCA_C
Cuts at: 4142 4142
Size: 5372
ApoI r'AATT_y
Cuts at: 2089 2376 4725 4981 2089
Size: 287 2349 256 2480
Fragments arranged by size:
2480 2349 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 799 1200 2392 799
Size: 401 1192 3779
Fragments arranged by size:
3779 1192 401
BamHI G'GATC_C
Cuts at: 2397 2397
Size: 5372
BanI G'GyrC_C
Cuts at: 1204 1381 1599 1663 1973 2272 2388 2802 3050
Size: 177 218 64 310 299 116 414 248
Cuts at: 3050 3085 1204
Size: 35 3491
Fragments arranged by size:
3491 414 310 299 248 218 177 116
64 35
BanII G_rGCy'C
Cuts at: 1171 1256 1477 1732 1918 2243 2386 3416 1171
Size: 85 221 255 186 325 143 1030 3127
Fragments arranged by size:
3127 1030 325 255 221 186 143 85
BbeI G_GCGC'C
Cuts at: 1385 2806 3054 1385
Size: 1421 248 3703
Fragments arranged by size:
3703 1421 248
BbvCI CC'TCA_GC
Cuts at: 280 1848 280
Size: 1568 3804
BciVI GTATCCnnnnn_n'
Cuts at: 3270 4031 5372 3270
Size: 761 1341 3270
Fragments arranged by size:
3270 1341 761
BclI T'GATC_A
Cuts at: 2892 2892
Size: 5372
BglII A'GATC_T
Cuts at: 863 2887 4924 863
Size: 2024 2037 1311
Fragments arranged by size:
2037 2024 1311
BlpI GC'TnA_GC
Cuts at: 2484 2484
Size: 5372
Bme1580I G_kGCm'C
Cuts at: 1164 1171 1207 1256 1477 1668 1918 1925 2149
Size: 7 36 49 221 191 250 7 224
Cuts at: 2149 2275 2997 3090 4146 1164
Size: 126 722 93 1056 2390
Fragments arranged by size:
2390 1056 722 250 224 221 191 126
93 49 36 7 7
BmrI ACTGGGnnnn_n'
Cuts at: 1241 1517 1921 2584 2665 3000 5144 1241
Size: 276 404 663 81 335 2144 1469
Fragments arranged by size:
2144 1469 663 404 335 276 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1133 3739 1133
Size: 2606 2766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 989 2161 2347 2368 888
Size: 101 1172 186 21 3892
Fragments arranged by size:
3892 1172 186 101 21
Bpu10I CC'TnA_GC
Cuts at: 280 1566 1718 1822 1848 280
Size: 1286 152 104 26 3804
Fragments arranged by size:
3804 1286 152 104 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1128 2548 3427 3919 4217 136
Size: 992 1420 879 492 298 1291
Fragments arranged by size:
1420 1291 992 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 391 652 5076 391
Size: 261 4424 687
Fragments arranged by size:
4424 687 261
BsaAI yAC'GTr
Cuts at: 645 2725 645
Size: 2080 3292
BsaBI GATnn'nnATC
Cuts at: 6 2886 4923 6
Size: 2880 2037 455
Fragments arranged by size:
2880 2037 455
BsaHI Gr'CG_yC
Cuts at: 701 1382 2041 2803 3051 3753 5249 701
Size: 681 659 762 248 702 1496 824
Fragments arranged by size:
1496 824 762 702 681 659 248
BsaWI w'CCGG_w
Cuts at: 599 2561 3082 3760 4034 4181 4592 599
Size: 1962 521 678 274 147 411 1379
Fragments arranged by size:
1962 1379 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 670 700 760 790 1197 1227 1577
Size: 30 102 30 60 30 407 30 350
Cuts at: 1577 1607 538
Size: 30 4303
Fragments arranged by size:
4303 407 350 102 60 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 781 2251 781
Size: 1470 3902
BseYI C'CCAG_C
Cuts at: 838 922 927 1056 1189 1255 1265 1279 1504
Size: 84 5 129 133 66 10 14 225
Cuts at: 1504 1546 1588 1621 1636 1853 1896 2797 3555
Size: 42 42 33 15 217 43 901 758
Cuts at: 3555 4132 4628 838
Size: 577 496 1582
Fragments arranged by size:
1582 901 758 577 496 225 217 133
129 84 66 43 42 42 33 15
14 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1780 580
Size: 1200 4172
BsiEI CG_ry'CG
Cuts at: 104 2111 2914 2960 4168 104
Size: 2007 803 46 1208 1308
Fragments arranged by size:
2007 1308 1208 803 46
BsiHKAI G_wGCw'C
Cuts at: 1114 1732 2386 3164 3354 4146 1114
Size: 618 654 778 190 792 2340
Fragments arranged by size:
2340 792 778 654 618 190
BsmBI CGTCTCn'nnnn_
Cuts at: 355 355
Size: 5372
Bsp1286I G_dGCh'C
Cuts at: 1114 1164 1171 1207 1256 1477 1668 1732 1918
Size: 50 7 36 49 221 191 64 186
Cuts at: 1918 1925 2149 2243 2275 2386 2997 3090 3164
Size: 7 224 94 32 111 611 93 74
Cuts at: 3164 3354 3416 4146 1114
Size: 190 62 730 2340
Fragments arranged by size:
2340 730 611 224 221 191 190 186
111 94 93 74 64 62 50 49
36 32 7 7
BspEI T'CCGG_A
Cuts at: 4592 4592
Size: 5372
BspHI T'CATG_A
Cuts at: 4548 4548
Size: 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 2422 2636 2938 3319 510
Size: 1912 214 302 381 2563
Fragments arranged by size:
2563 1912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3664 3718 5202 5367 50
Size: 3614 54 1484 165 55
Fragments arranged by size:
3614 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3284 3284
Size: 5372
BsrFI r'CCGG_y
Cuts at: 1354 1660 3370 3551 3760 1354
Size: 306 1710 181 209 2966
Fragments arranged by size:
2966 1710 306 209 181
BssSI C'ACGA_G
Cuts at: 2233 3643 4001 2233
Size: 1410 358 3604
Fragments arranged by size:
3604 1410 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1980 2749 1663
Size: 317 769 4286
Fragments arranged by size:
4286 769 317
BstBI TT'CG_AA
Cuts at: 3733 3733
Size: 5372
BstXI CCAn_nnnn'nTGG
Cuts at: 1596 1596
Size: 5372
BstYI r'GATC_y
Cuts at: 1 450 863 2397 2864 2887 3222 3468 4480
Size: 449 413 1534 467 23 335 246 1012
Cuts at: 4480 4566 4578 4924 1
Size: 86 12 346 449
Fragments arranged by size:
1534 1012 467 449 449 413 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 941 1742 1955 107
Size: 834 801 213 3524
Fragments arranged by size:
3524 834 801 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3322 3503 3666 3322
Size: 181 163 5028
Fragments arranged by size:
5028 181 163
BtsI GCAGTG_nn'
Cuts at: 1356 1446 1603 2615 1356
Size: 90 157 1012 4113
Fragments arranged by size:
4113 1012 157 90
DraI TTT'AAA
Cuts at: 2371 2371
Size: 5372
DrdI GACnn_nn'nnGTC
Cuts at: 991 3078 3936 991
Size: 2087 858 2427
Fragments arranged by size:
2427 2087 858
EaeI y'GGCC_r
Cuts at: 480 2957 3131 3522 3549 4894 480
Size: 2477 174 391 27 1345 958
Fragments arranged by size:
2477 1345 958 391 174 27
EagI C'GGCC_G
Cuts at: 2957 2957
Size: 5372
EarI CTCTTCn'nnn_
Cuts at: 467 961 1955 2125 3395 3605 467
Size: 494 994 170 1270 210 2234
Fragments arranged by size:
2234 1270 994 494 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1316 1337 1762 3580 3890 4036 5182 127
Size: 1189 21 425 1818 310 146 1146 317
Fragments arranged by size:
1818 1189 1146 425 317 310 146 21
EcoICRI GAG'CTC
Cuts at: 1730 2384 1730
Size: 654 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 974 989 1626 2161 2347 2368 3197 3629
Size: 86 15 637 535 186 21 829 432
Cuts at: 3629 4376 4644 888
Size: 747 268 1616
Fragments arranged by size:
1616 829 747 637 535 432 268 186
86 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 154 253 1295 1930 154
Size: 99 1042 635 3596
Fragments arranged by size:
3596 1042 635 99
EcoO109I rG'GnC_Cy
Cuts at: 426 753 1134 1168 1474 1585 1713 1779 1915
Size: 327 381 34 306 111 128 66 136
Cuts at: 1915 2175 2358 2511 426
Size: 260 183 153 3287
Fragments arranged by size:
3287 381 327 306 260 183 153 136
128 111 66 34
EcoRI G'AATT_C
Cuts at: 2089 2376 2089
Size: 287 5085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2340 3391 2340
Size: 1051 4321
FspI TGC'GCA
Cuts at: 649 670 3153 649
Size: 21 2483 2868
Fragments arranged by size:
2868 2483 21
FspAI rTGC'GCAy
Cuts at: 649 649
Size: 5372
HaeII r_GCGC'y
Cuts at: 820 1385 2103 2806 3054 4076 5055 820
Size: 565 718 703 248 1022 979 1137
Fragments arranged by size:
1137 1022 979 718 703 565 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 670 702 1577 1609 1802 1834 3214
Size: 32 100 32 875 32 193 32 1380
Cuts at: 3214 3244 3246 3276 538
Size: 30 2 30 2634
Fragments arranged by size:
2634 1380 875 193 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 699 741 2108 2411 699
Size: 42 1367 303 3660
Fragments arranged by size:
3660 1367 303 42
HindIII A'AGCT_T
Cuts at: 410 410
Size: 5372
KasI G'GCGC_C
Cuts at: 1381 2802 3050 1381
Size: 1421 248 3703
Fragments arranged by size:
3703 1421 248
KpnI G_GTAC'C
Cuts at: 2392 2392
Size: 5372
MluI A'CGCG_T
Cuts at: 4805 4805
Size: 5372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 2062 4043 4227 335
Size: 1727 1981 184 1480
Fragments arranged by size:
1981 1727 1480 184
MscI TGG'CCA
Cuts at: 482 3133 482
Size: 2651 2721
MslI CAynn'nnrTG
Cuts at: 520 3361 3488 520
Size: 2841 127 2404
Fragments arranged by size:
2841 2404 127
MspA1I CmG'CkG
Cuts at: 1060 1316 1869 1890 2310 2483 2797 3157 4170
Size: 256 553 21 420 173 314 360 1013
Cuts at: 4170 4415 1060
Size: 245 2017
Fragments arranged by size:
2017 1013 553 420 360 314 256 245
173 21
NaeI GCC'GGC
Cuts at: 3553 3553
Size: 5372
NarI GG'CG_CC
Cuts at: 1382 2803 3051 1382
Size: 1421 248 3703
Fragments arranged by size:
3703 1421 248
NcoI C'CATG_G
Cuts at: 107 1742 1955 107
Size: 1635 213 3524
Fragments arranged by size:
3524 1635 213
NgoMIV G'CCGG_C
Cuts at: 3551 3551
Size: 5372
NspI r_CATG'y
Cuts at: 519 654 675 1983 2425 2925 3832 519
Size: 135 21 1308 442 500 907 2059
Fragments arranged by size:
2059 1308 907 500 442 135 21
PasI CC'CwG_GG
Cuts at: 1164 1164
Size: 5372
PciI A'CATG_T
Cuts at: 3828 3828
Size: 5372
PflMI CCAn_nnn'nTGG
Cuts at: 1230 1470 1230
Size: 240 5132
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PmlI CAC'GTG
Cuts at: 645 645
Size: 5372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 992 4511 4543 960
Size: 32 3519 32 1789
Fragments arranged by size:
3519 1789 32 32
PpuMI rG'GwC_Cy
Cuts at: 753 1134 1585 1779 2358 753
Size: 381 451 194 579 3767
Fragments arranged by size:
3767 579 451 381 194
PspOMI G'GGCC_C
Cuts at: 1167 1252 1473 1914 1167
Size: 85 221 441 4625
Fragments arranged by size:
4625 441 221 85
PspXI vC'TCGA_Gb
Cuts at: 799 799
Size: 5372
PstI C_TGCA'G
Cuts at: 1868 2419 3104 1868
Size: 551 685 4136
Fragments arranged by size:
4136 685 551
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5372
PvuII CAG'CTG
Cuts at: 1060 1869 2797 3157 1060
Size: 809 928 360 3275
Fragments arranged by size:
3275 928 809 360
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 1732 2386 1732
Size: 654 4718
SalI G'TCGA_C
Cuts at: 2106 2409 2106
Size: 303 5069
SanDI GG'GwC_CC
Cuts at: 1134 1585 1134
Size: 451 4921
SapI GCTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 733 4889 733
Size: 4156 1216
SfcI C'TryA_G
Cuts at: 33 1864 2415 3100 4093 4284 33
Size: 1831 551 685 993 191 1121
Fragments arranged by size:
1831 1121 993 685 551 191
SfoI GGC'GCC
Cuts at: 1383 2804 3052 1383
Size: 1421 248 3703
Fragments arranged by size:
3703 1421 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 2394 2394
Size: 5372
SmlI C'TyrA_G
Cuts at: 151 799 1107 2527 3442 3934 4196 151
Size: 648 308 1420 915 492 262 1327
Fragments arranged by size:
1420 1327 915 648 492 308 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 654 675 2425 2925 654
Size: 21 1750 500 3101
Fragments arranged by size:
3101 1750 500 21
StuI AGG'CCT
Cuts at: 1468 1468
Size: 5372
StyI C'CwwG_G
Cuts at: 107 129 1172 1469 1652 1742 1931 1955 2171
Size: 22 1043 297 183 90 189 24 216
Cuts at: 2171 2506 2860 107
Size: 335 354 2619
Fragments arranged by size:
2619 1043 354 335 297 216 189 183
90 24 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1819 1888 2125 2955 3088 3547 3756 1819
Size: 69 237 830 133 459 209 3435
Fragments arranged by size:
3435 830 459 237 209 133 69
TatI w'GTAC_w
Cuts at: 731 1828 2210 4887 731
Size: 1097 382 2677 1216
Fragments arranged by size:
2677 1216 1097 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1036 1068 1197 1229 4889 4921 1036
Size: 32 129 32 3660 32 1487
Fragments arranged by size:
3660 1487 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3169 3169
Size: 5372
XbaI T'CTAG_A
Cuts at: 2403 2403
Size: 5372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2867 2867
Size: 5372
XhoI C'TCGA_G
Cuts at: 799 799
Size: 5372
XmaI C'CCGG_G
Cuts at: 2392 2392
Size: 5372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI