Restriction map for
pF1KA0700
(Circular) (Six-base) MAPSORT of: pF1KA0700.seq Check: 7084 from: 1 to: 6503
pF1KA0700 6503 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 2889 3258 525
Size: 2364 369 3770
Fragments arranged by size:
3770 2364 369
AatII G_ACGT'C
Cuts at: 2726 2726
Size: 6503
AccI GT'mk_AC
Cuts at: 1904 2324 3541 1904
Size: 420 1217 4866
Fragments arranged by size:
4866 1217 420
Acc65I G'GTAC_C
Cuts at: 976 3405 3519 976
Size: 2429 114 3960
Fragments arranged by size:
3960 2429 114
AclI AA'CG_TT
Cuts at: 6343 6343
Size: 6503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1081 1753 2419 2539 2752 4328 4760 5113
Size: 326 672 666 120 213 1576 432 353
Cuts at: 5113 5775 755
Size: 662 1483
Fragments arranged by size:
1576 1483 672 666 662 432 353 326
213 120
AfeI AGC'GCT
Cuts at: 913 1483 2012 2845 913
Size: 570 529 833 4571
Fragments arranged by size:
4571 833 570 529
AflIII A'CryG_T
Cuts at: 360 680 3233 3855 5655 5936 360
Size: 320 2553 622 1800 281 927
Fragments arranged by size:
2553 1800 927 622 320 281
AleI CACnn'nnGTG
Cuts at: 754 1469 754
Size: 715 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2705 2705
Size: 6503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1408 1440 2776 2808 1408
Size: 32 1336 32 5103
Fragments arranged by size:
5103 1336 32 32
AlwNI CAG_nnn'CTG
Cuts at: 810 1119 1995 2082 2121 2382 2430 2970 3279
Size: 309 876 87 39 261 48 540 309
Cuts at: 3279 3326 3808 5246 810
Size: 47 482 1438 2067
Fragments arranged by size:
2067 1438 876 540 482 309 309 261
87 48 47 39
ApaI G_GGCC'C
Cuts at: 3111 3111
Size: 6503
ApaLI G'TGCA_C
Cuts at: 223 3433 3439 5341 223
Size: 3210 6 1902 1385
Fragments arranged by size:
3210 1902 1385 6
ApoI r'AATT_y
Cuts at: 492 589 1027 1168 3507 5856 6112 492
Size: 97 438 141 2339 2349 256 883
Fragments arranged by size:
2349 2339 883 438 256 141 97
AseI AT'TA_AT
Cuts at: 20 20
Size: 6503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
AvaI C'yCGr_G
Cuts at: 192 3523 192
Size: 3331 3172
BamHI G'GATC_C
Cuts at: 1300 3528 1300
Size: 2228 4275
BanI G'GyrC_C
Cuts at: 142 175 537 564 976 1566 2065 2131 2211
Size: 33 362 27 412 590 499 66 80
Cuts at: 2211 2770 3405 3519 3933 4181 4216 142
Size: 559 635 114 414 248 35 2429
Fragments arranged by size:
2429 635 590 559 499 414 412 362
248 114 80 66 35 33 27
BanII G_rGCy'C
Cuts at: 199 1139 2747 2921 3111 3133 3517 4547 199
Size: 940 1608 174 190 22 384 1030 2155
Fragments arranged by size:
2155 1608 1030 940 384 190 174 22
BbeI G_GCGC'C
Cuts at: 179 1570 2135 2215 3937 4185 179
Size: 1391 565 80 1722 248 2497
Fragments arranged by size:
2497 1722 1391 565 248 80
BbsI GAAGACnn'nnnn_
Cuts at: 239 1447 1580 239
Size: 1208 133 5162
Fragments arranged by size:
5162 1208 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2956 2990 2956
Size: 34 6469
BciVI GTATCCnnnnn_n'
Cuts at: 4401 5457 6503 4401
Size: 1056 1046 4401
Fragments arranged by size:
4401 1056 1046
BclI T'GATC_A
Cuts at: 2903 4023 2903
Size: 1120 5383
BfrBI ATG'CAT
Cuts at: 1282 1282
Size: 6503
BglI GCCn_nnn'nGGC
Cuts at: 158 158
Size: 6503
BglII A'GATC_T
Cuts at: 1781 2402 4018 6055 1781
Size: 621 1616 2037 2229
Fragments arranged by size:
2229 2037 1616 621
BlpI GC'TnA_GC
Cuts at: 169 2840 3190 3615 169
Size: 2671 350 425 3057
Fragments arranged by size:
3057 2671 425 350
Bme1580I G_kGCm'C
Cuts at: 227 569 1317 1947 2068 2099 3111 3437 3443
Size: 342 748 630 121 31 1012 326 6
Cuts at: 3443 4128 4221 5345 227
Size: 685 93 1124 1385
Fragments arranged by size:
1385 1124 1012 748 685 630 342 326
121 93 31 6
BmgBI CAC'GTC
Cuts at: 361 1616 3171 361
Size: 1255 1555 3693
Fragments arranged by size:
3693 1555 1255
BmrI ACTGGGnnnn_n'
Cuts at: 2186 2522 3715 3796 4131 6275 2186
Size: 336 1193 81 335 2144 2414
Fragments arranged by size:
2414 2144 1193 336 335 81
BmtI G_CTAG'C
Cuts at: 3362 3362
Size: 6503
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1944 4870 1944
Size: 2926 3577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 368 592 688 1525 2017 2373 2452 2563
Size: 43 224 96 837 492 356 79 111
Cuts at: 2563 2608 3352 325
Size: 45 744 3476
Fragments arranged by size:
3476 837 744 492 356 224 111 96
79 45 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 1798 3052 3679 4558 5272 5570 1672
Size: 126 1254 627 879 714 298 2605
Fragments arranged by size:
2605 1254 879 714 627 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 833 1398 2812 6207 833
Size: 565 1414 3395 1129
Fragments arranged by size:
3395 1414 1129 565
BsaAI yAC'GTr
Cuts at: 228 683 2028 2655 2982 3438 3856 228
Size: 455 1345 627 327 456 418 2875
Fragments arranged by size:
2875 1345 627 456 455 418 327
BsaBI GATnn'nnATC
Cuts at: 6 4017 6054 6
Size: 4011 2037 455
Fragments arranged by size:
4011 2037 455
BsaHI Gr'CG_yC
Cuts at: 176 236 1567 2132 2212 2723 3934 4182 4884
Size: 60 1331 565 80 511 1211 248 702
Cuts at: 4884 6380 176
Size: 1496 299
Fragments arranged by size:
1496 1331 1211 702 565 511 299 248
80 60
BsaWI w'CCGG_w
Cuts at: 3692 4213 4891 5302 5449 5723 3692
Size: 521 678 411 147 274 4472
Fragments arranged by size:
4472 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1108 1138 2776 2806 1108
Size: 30 1638 30 4805
Fragments arranged by size:
4805 1638 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 919 1468 1762 2155 2254 2560 2947 3325
Size: 165 549 294 393 99 306 387 378
Cuts at: 3325 3403 754
Size: 78 3854
Fragments arranged by size:
3854 549 393 387 378 306 294 165
99 78
BseYI C'CCAG_C
Cuts at: 182 1763 2098 2295 3928 4686 5351 5759 182
Size: 1581 335 197 1633 758 665 408 926
Fragments arranged by size:
1633 1581 926 758 665 408 335 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 661 889 929 1365 1564 2068 2356 2686
Size: 125 228 40 436 199 504 288 330
Cuts at: 2686 2701 3292 3388 3490 536
Size: 15 591 96 102 3549
Fragments arranged by size:
3549 591 504 436 330 288 228 199
125 102 96 40 15
BsiEI CG_ry'CG
Cuts at: 104 4045 4091 5321 104
Size: 3941 46 1230 1286
Fragments arranged by size:
3941 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 227 386 884 902 1139 1916 2389 2747 2921
Size: 159 498 18 237 777 473 358 174
Cuts at: 2921 3133 3437 3443 3517 4295 4485 5345 227
Size: 212 304 6 74 778 190 860 1385
Fragments arranged by size:
1385 860 778 777 498 473 358 304
237 212 190 174 159 74 18 6
BsiWI C'GTAC_G
Cuts at: 1448 1448
Size: 6503
BsmBI CGTCTCn'nnnn_
Cuts at: 1501 1501
Size: 6503
Bsp1286I G_dGCh'C
Cuts at: 199 227 386 569 884 902 1139 1317 1916
Size: 28 159 183 315 18 237 178 599
Cuts at: 1916 1947 2068 2099 2389 2747 2921 3111 3133
Size: 31 121 31 290 358 174 190 22
Cuts at: 3133 3437 3443 3517 4128 4221 4295 4485 4547
Size: 304 6 74 611 93 74 190 62
Cuts at: 4547 5345 199
Size: 798 1357
Fragments arranged by size:
1357 798 611 599 358 315 304 290
237 190 190 183 178 174 159 121
93 74 74 62 31 31 28 22
18 6
BspHI T'CATG_A
Cuts at: 311 2906 4935 311
Size: 2595 2029 1879
Fragments arranged by size:
2595 2029 1879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 794 2889 3258 3553 3767 4069 4450 525
Size: 269 2095 369 295 214 302 381 2578
Fragments arranged by size:
2578 2095 381 369 302 295 269 214
BsrBI CCG'CTC
Cuts at: 50 173 2199 2454 4795 4849 6333 6498 50
Size: 123 2026 255 2341 54 1484 165 55
Fragments arranged by size:
2341 2026 1484 255 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 2687 4415 2687
Size: 1728 4775
BsrFI r'CCGG_y
Cuts at: 145 219 713 1985 2040 2208 3083 4501 4682
Size: 74 494 1272 55 168 875 1418 181
Cuts at: 4682 145
Size: 1966
Fragments arranged by size:
1966 1418 1272 875 494 181 168 74
55
BssSI C'ACGA_G
Cuts at: 208 379 705 2710 4774 5482 208
Size: 171 326 2005 2064 708 1229
Fragments arranged by size:
2064 2005 1229 708 326 171
BstAPI GCAn_nnn'nTGC
Cuts at: 537 867 3447 3880 537
Size: 330 2580 433 3160
Fragments arranged by size:
3160 2580 433 330
BstBI TT'CG_AA
Cuts at: 4864 4864
Size: 6503
BstXI CCAn_nnnn'nTGG
Cuts at: 2535 2535
Size: 6503
BstYI r'GATC_y
Cuts at: 1 263 671 1246 1300 1781 1991 2402 3528
Size: 262 408 575 54 481 210 411 1126
Cuts at: 3528 3995 4018 4353 4599 4905 4917 5003 6055
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6055 1
Size: 449
Fragments arranged by size:
1126 1052 575 481 467 449 411 408
335 306 262 246 210 86 54 23
12
BtgI C'CryG_G
Cuts at: 107 155 504 584 2504 2666 107
Size: 48 349 80 1920 162 3944
Fragments arranged by size:
3944 1920 349 162 80 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 978 1083 2732 4453 4634 4797 978
Size: 105 1649 1721 181 163 2684
Fragments arranged by size:
2684 1721 1649 181 163 105
BtsI GCAGTG_nn'
Cuts at: 1007 1353 1947 3746 1007
Size: 346 594 1799 3764
Fragments arranged by size:
3764 1799 594 346
ClaI AT'CG_AT
Cuts at: 338 338
Size: 6503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1329 1364 1329
Size: 35 6468
DraI TTT'AAA
Cuts at: 3502 3502
Size: 6503
DraIII CAC_nnn'GTG
Cuts at: 867 2712 867
Size: 1845 4658
DrdI GACnn_nn'nnGTC
Cuts at: 1390 4209 5553 1390
Size: 2819 1344 2340
Fragments arranged by size:
2819 2340 1344
EaeI y'GGCC_r
Cuts at: 715 758 1517 2042 2267 2852 4088 4262 4653
Size: 43 759 525 225 585 1236 174 391
Cuts at: 4653 4680 6025 715
Size: 27 1345 1193
Fragments arranged by size:
1345 1236 1193 759 585 525 391 225
174 43 27
EagI C'GGCC_G
Cuts at: 4088 4088
Size: 6503
EarI CTCTTCn'nnn_
Cuts at: 379 731 772 1057 1111 1739 2110 4526 4736
Size: 352 41 285 54 628 371 2416 210
Cuts at: 4736 379
Size: 2146
Fragments arranged by size:
2416 2146 628 371 352 285 210 54
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 922 1039 3067 3280 4711 5453 5599 6313 922
Size: 117 2028 213 1431 742 146 714 1112
Fragments arranged by size:
2028 1431 1112 742 714 213 146 117
EcoICRI GAG'CTC
Cuts at: 1137 2745 2919 3131 3515 1137
Size: 1608 174 212 384 4125
Fragments arranged by size:
4125 1608 384 212 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 368 592 688 755 1081 1525 1753 2017
Size: 43 224 96 67 326 444 228 264
Cuts at: 2017 2373 2419 2452 2539 2563 2608 2752 3352
Size: 356 46 33 87 24 45 144 600
Cuts at: 3352 4328 4760 5113 5775 325
Size: 976 432 353 662 1053
Fragments arranged by size:
1053 976 662 600 444 432 356 353
326 264 228 224 144 96 87 67
46 45 43 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2587 3367 2587
Size: 780 5723
EcoO109I rG'GnC_Cy
Cuts at: 783 1665 1863 2218 2249 2949 3108 3248 3642
Size: 882 198 355 31 700 159 140 394
Cuts at: 3642 783
Size: 3644
Fragments arranged by size:
3644 882 700 394 355 198 159 140
31
EcoRI G'AATT_C
Cuts at: 492 589 3507 492
Size: 97 2918 3488
Fragments arranged by size:
3488 2918 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 2268 4522 285
Size: 1983 2254 2266
Fragments arranged by size:
2266 2254 1983
FseI GG_CCGG'CC
Cuts at: 717 717
Size: 6503
FspI TGC'GCA
Cuts at: 4284 4284
Size: 6503
HaeII r_GCGC'y
Cuts at: 179 915 1485 1570 1635 2014 2135 2215 2847
Size: 736 570 85 65 379 121 80 632
Cuts at: 2847 3164 3937 4185 5415 6186 179
Size: 317 773 248 1230 771 496
Fragments arranged by size:
1230 773 771 736 632 570 496 379
317 248 121 85 80 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 1108 1140 1267 1299 2995 3027 4345
Size: 32 301 32 127 32 1696 32 1318
Cuts at: 4345 4375 4377 4407 775
Size: 30 2 30 2871
Fragments arranged by size:
2871 1696 1318 301 127 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3542 3542
Size: 6503
KasI G'GCGC_C
Cuts at: 175 1566 2131 2211 3933 4181 175
Size: 1391 565 80 1722 248 2497
Fragments arranged by size:
2497 1722 1391 565 248 80
KpnI G_GTAC'C
Cuts at: 980 3409 3523 980
Size: 2429 114 3960
Fragments arranged by size:
3960 2429 114
MluI A'CGCG_T
Cuts at: 5936 5936
Size: 6503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 381 1514 1598 5262 5446 381
Size: 1133 84 3664 184 1438
Fragments arranged by size:
3664 1438 1133 184 84
MscI TGG'CCA
Cuts at: 760 1519 4264 760
Size: 759 2745 2999
Fragments arranged by size:
2999 2745 759
MslI CAynn'nnrTG
Cuts at: 754 1469 1972 4492 4619 754
Size: 715 503 2520 127 2638
Fragments arranged by size:
2638 2520 715 503 127
MspA1I CmG'CkG
Cuts at: 131 140 157 182 699 1094 1469 1708 2079
Size: 9 17 25 517 395 375 239 371
Cuts at: 2079 2121 2149 2700 2776 2798 3326 3614 3928
Size: 42 28 551 76 22 528 288 314
Cuts at: 3928 4288 5072 5317 131
Size: 360 784 245 1317
Fragments arranged by size:
1317 784 551 528 517 395 375 371
360 314 288 245 239 76 42 28
25 22 17 9
NaeI GCC'GGC
Cuts at: 147 715 2042 2210 4684 147
Size: 568 1327 168 2474 1966
Fragments arranged by size:
2474 1966 1327 568 168
NarI GG'CG_CC
Cuts at: 176 1567 2132 2212 3934 4182 176
Size: 1391 565 80 1722 248 2497
Fragments arranged by size:
2497 1722 1391 565 248 80
NcoI C'CATG_G
Cuts at: 107 2504 2666 107
Size: 2397 162 3944
Fragments arranged by size:
3944 2397 162
NgoMIV G'CCGG_C
Cuts at: 145 713 2040 2208 4682 145
Size: 568 1327 168 2474 1966
Fragments arranged by size:
2474 1966 1327 568 168
NheI G'CTAG_C
Cuts at: 3358 3358
Size: 6503
NsiI A_TGCA'T
Cuts at: 1284 1284
Size: 6503
NspI r_CATG'y
Cuts at: 732 3237 3556 4056 5659 732
Size: 2505 319 500 1603 1576
Fragments arranged by size:
2505 1603 1576 500 319
PasI CC'CwG_GG
Cuts at: 1605 2172 1605
Size: 567 5936
PciI A'CATG_T
Cuts at: 3233 5655 3233
Size: 2422 4081
PflMI CCAn_nnn'nTGG
Cuts at: 1119 2970 1119
Size: 1851 4652
PfoI T'CCnGG_A
Cuts at: 302 345 665 1502 1994 2564 2921 3133 302
Size: 43 320 837 492 570 357 212 3672
Fragments arranged by size:
3672 837 570 492 357 320 212 43
PmeI GTTT'AAAC
Cuts at: 3502 3502
Size: 6503
PmlI CAC'GTG
Cuts at: 3438 3438
Size: 6503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 730 762 2776 2808 4949 4981 730
Size: 32 2014 32 2141 32 2252
Fragments arranged by size:
2252 2141 2014 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 783 2218 2949 3248 783
Size: 1435 731 299 4038
Fragments arranged by size:
4038 1435 731 299
PspOMI G'GGCC_C
Cuts at: 3107 3107
Size: 6503
PstI C_TGCA'G
Cuts at: 1002 1025 1145 2888 3059 3550 4235 1002
Size: 23 120 1743 171 491 685 3270
Fragments arranged by size:
3270 1743 685 491 171 120 23
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6503
PvuII CAG'CTG
Cuts at: 699 2079 2121 2149 2700 3928 4288 699
Size: 1380 42 28 551 1228 360 2914
Fragments arranged by size:
2914 1380 1228 551 360 42 28
RsrII CG'GwC_CG
Cuts at: 2441 4698 2441
Size: 2257 4246
SacI G_AGCT'C
Cuts at: 1139 2747 2921 3133 3517 1139
Size: 1608 174 212 384 4125
Fragments arranged by size:
4125 1608 384 212 174
SacII CC_GC'GG
Cuts at: 158 158
Size: 6503
SalI G'TCGA_C
Cuts at: 3540 3540
Size: 6503
SanDI GG'GwC_CC
Cuts at: 3248 3248
Size: 6503
SapI GCTCTTCn'nnn_
Cuts at: 379 1057 4526 4736 379
Size: 678 3469 210 2146
Fragments arranged by size:
3469 2146 678 210
SbfI CC_TGCA'GG
Cuts at: 3550 3550
Size: 6503
ScaI AGT'ACT
Cuts at: 2551 6020 2551
Size: 3469 3034
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 6503
SfcI C'TryA_G
Cuts at: 33 654 998 1021 1141 2325 2884 3055 3546
Size: 621 344 23 120 1184 559 171 491
Cuts at: 3546 4231 5199 5390 33
Size: 685 968 191 1146
Fragments arranged by size:
1184 1146 968 685 621 559 491 344
191 171 120 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 158 158
Size: 6503
SfoI GGC'GCC
Cuts at: 177 1568 2133 2213 3935 4183 177
Size: 1391 565 80 1722 248 2497
Fragments arranged by size:
2497 1722 1391 565 248 80
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
SgrAI Cr'CCGG_yG
Cuts at: 3083 3083
Size: 6503
SmaI CCC'GGG
Cuts at: 3525 3525
Size: 6503
SmlI C'TyrA_G
Cuts at: 1687 1813 3067 3658 4573 5287 5549 1687
Size: 126 1254 591 915 714 262 2641
Fragments arranged by size:
2641 1254 915 714 591 262 126
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6503
SphI G_CATG'C
Cuts at: 3556 4056 3556
Size: 500 6003
StyI C'CwwG_G
Cuts at: 107 1739 1859 2504 2666 3637 3991 107
Size: 1632 120 645 162 971 354 2619
Fragments arranged by size:
2619 1632 971 645 354 162 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 659 1248 2458 3016 3373 4086 4219 4678 4887
Size: 589 1210 558 357 713 133 459 209
Cuts at: 4887 659
Size: 2275
Fragments arranged by size:
2275 1210 713 589 558 459 357 209
133
TatI w'GTAC_w
Cuts at: 2549 3473 6018 2549
Size: 924 2545 3034
Fragments arranged by size:
3034 2545 924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1735 1767 6020 6052 1735
Size: 32 4253 32 2186
Fragments arranged by size:
4253 2186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1045 1498 2320 4300 1045
Size: 453 822 1980 3248
Fragments arranged by size:
3248 1980 822 453
XbaI T'CTAG_A
Cuts at: 3534 3534
Size: 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 999 2673 2919 3998 999
Size: 1674 246 1079 3504
Fragments arranged by size:
3504 1674 1079 246
XmaI C'CCGG_G
Cuts at: 3523 3523
Size: 6503
XmnI GAAnn'nnTTC
Cuts at: 319 2866 319
Size: 2547 3956
ZraI GAC'GTC
Cuts at: 2724 2724
Size: 6503
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AscI AvrII BaeI BbvCI Bpu10I
BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I EcoRV
FspAI HindIII HpaI MfeI NdeI NotI NruI PacI
PshAI PsiI PspXI PsrI SnaBI SrfI SspI StuI
SwaI XhoI