Restriction map for
pF1KA0705
(Circular) (Six-base) MAPSORT of: pF1KA0705.seq Check: 4249 from: 1 to: 7436
pF1KA0705 7436 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 880 1449 2277 880
Size: 569 828 6039
Fragments arranged by size:
6039 828 569
AccI GT'mk_AC
Cuts at: 1061 4474 1061
Size: 3413 4023
Acc65I G'GTAC_C
Cuts at: 658 4452 658
Size: 3794 3642
AclI AA'CG_TT
Cuts at: 7276 7276
Size: 7436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 466 761 976 1013 1676 2418 2810 3229
Size: 243 295 215 37 663 742 392 419
Cuts at: 3229 5261 5693 6046 6708 223
Size: 2032 432 353 662 951
Fragments arranged by size:
2032 951 742 663 662 432 419 392
353 295 243 215 37
AfeI AGC'GCT
Cuts at: 4012 4012
Size: 7436
AflII C'TTAA_G
Cuts at: 2104 2104
Size: 7436
AflIII A'CryG_T
Cuts at: 4788 6588 6869 4788
Size: 1800 281 5355
Fragments arranged by size:
5355 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1878 1878
Size: 7436
AlwNI CAG_nnn'CTG
Cuts at: 2076 2256 2698 4741 6179 2076
Size: 180 442 2043 1438 3333
Fragments arranged by size:
3333 2043 1438 442 180
ApaI G_GGCC'C
Cuts at: 4312 4364 4312
Size: 52 7384
ApaLI G'TGCA_C
Cuts at: 6274 6274
Size: 7436
ApoI r'AATT_y
Cuts at: 4440 6789 7045 4440
Size: 2349 256 4831
Fragments arranged by size:
4831 2349 256
AseI AT'TA_AT
Cuts at: 20 1535 20
Size: 1515 5921
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7436
AvaI C'yCGr_G
Cuts at: 1349 2443 3994 4048 4078 4114 4212 4239 4320
Size: 1094 1551 54 30 36 98 27 81
Cuts at: 4320 4456 1349
Size: 136 4329
Fragments arranged by size:
4329 1551 1094 136 98 81 54 36
30 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 642 675 642
Size: 33 7403
BamHI G'GATC_C
Cuts at: 1101 2899 2987 4461 1101
Size: 1798 88 1474 4076
Fragments arranged by size:
4076 1798 1474 88
BanI G'GyrC_C
Cuts at: 211 519 658 892 1677 1939 3117 4056 4110
Size: 308 139 234 785 262 1178 939 54
Cuts at: 4110 4167 4327 4371 4411 4452 4866 5114 5149
Size: 57 160 44 40 41 414 248 35
Cuts at: 5149 211
Size: 2498
Fragments arranged by size:
2498 1178 939 785 414 308 262 248
234 160 139 57 54 44 41 40
35
BanII G_rGCy'C
Cuts at: 338 2287 2342 2500 2669 3101 4121 4189 4196
Size: 1949 55 158 169 432 1020 68 7
Cuts at: 4196 4312 4364 4450 5480 338
Size: 116 52 86 1030 2294
Fragments arranged by size:
2294 1949 1030 1020 432 169 158 116
86 68 55 52 7
BbeI G_GCGC'C
Cuts at: 215 4060 4114 4171 4331 4415 4870 5118 215
Size: 3845 54 57 160 84 455 248 2533
Fragments arranged by size:
3845 2533 455 248 160 84 57 54
BbsI GAAGACnn'nnnn_
Cuts at: 650 884 1001 1664 1734 3726 650
Size: 234 117 663 70 1992 4360
Fragments arranged by size:
4360 1992 663 234 117 70
BbvCI CC'TCA_GC
Cuts at: 1381 1381
Size: 7436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 475 509 475
Size: 34 7402
BciVI GTATCCnnnnn_n'
Cuts at: 1862 2712 5334 6390 7436 1862
Size: 850 2622 1056 1046 1862
Fragments arranged by size:
2622 1862 1056 1046 850
BclI T'GATC_A
Cuts at: 362 4956 362
Size: 4594 2842
BfrBI ATG'CAT
Cuts at: 1357 1357
Size: 7436
BglI GCCn_nnn'nGGC
Cuts at: 191 4102 4244 191
Size: 3911 142 3383
Fragments arranged by size:
3911 3383 142
BglII A'GATC_T
Cuts at: 4951 6988 4951
Size: 2037 5399
BlpI GC'TnA_GC
Cuts at: 2499 4548 2499
Size: 2049 5387
Bme1580I G_kGCm'C
Cuts at: 1944 2918 4312 4364 5061 5154 6278 1944
Size: 974 1394 52 697 93 1124 3102
Fragments arranged by size:
3102 1394 1124 974 697 93 52
BmgBI CAC'GTC
Cuts at: 351 351
Size: 7436
BmrI ACTGGGnnnn_n'
Cuts at: 1038 1356 2762 4648 4729 5064 7208 1038
Size: 318 1406 1886 81 335 2144 1266
Fragments arranged by size:
2144 1886 1406 1266 335 318 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 927 1724 5803 927
Size: 797 4079 2560
Fragments arranged by size:
4079 2560 797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 581 1115 1694 3734 3807 4285 328
Size: 253 534 579 2040 73 478 3479
Fragments arranged by size:
3479 2040 579 534 478 253 73
Bpu10I CC'TnA_GC
Cuts at: 1381 2077 1381
Size: 696 6740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 3724 4612 5491 6205 6503 376
Size: 3348 888 879 714 298 1309
Fragments arranged by size:
3348 1309 888 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 313 2975 7140 313
Size: 2662 4165 609
Fragments arranged by size:
4165 2662 609
BsaAI yAC'GTr
Cuts at: 1850 4789 1850
Size: 2939 4497
BsaBI GATnn'nnATC
Cuts at: 6 2402 4950 6987 6
Size: 2396 2548 2037 455
Fragments arranged by size:
2548 2396 2037 455
BsaHI Gr'CG_yC
Cuts at: 212 2376 3830 4057 4111 4168 4328 4412 4867
Size: 2164 1454 227 54 57 160 84 455
Cuts at: 4867 5115 5817 7313 212
Size: 248 702 1496 335
Fragments arranged by size:
2164 1496 1454 702 455 335 248 227
160 84 57 54
BsaWI w'CCGG_w
Cuts at: 1476 2984 4625 5146 5824 6235 6382 6656 1476
Size: 1508 1641 521 678 411 147 274 2256
Fragments arranged by size:
2256 1641 1508 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 401 431 836 866 2269 2299 401
Size: 30 405 30 1403 30 5538
Fragments arranged by size:
5538 1403 405 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 820 964 2165 3109 4066 310
Size: 510 144 1201 944 957 3680
Fragments arranged by size:
3680 1201 957 944 510 144
BseYI C'CCAG_C
Cuts at: 189 914 1166 3121 3185 3195 3221 4393 4861
Size: 725 252 1955 64 10 26 1172 468
Cuts at: 4861 5619 6284 6692 189
Size: 758 665 408 933
Fragments arranged by size:
1955 1172 933 758 725 665 468 408
252 64 26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 911 1798 908
Size: 3 887 6546
Fragments arranged by size:
6546 887 3
BsiEI CG_ry'CG
Cuts at: 104 1801 4042 4306 4421 4978 5024 6254 104
Size: 1697 2241 264 115 557 46 1230 1286
Fragments arranged by size:
2241 1697 1286 1230 557 264 115 46
BsiHKAI G_wGCw'C
Cuts at: 621 2287 2342 3101 4196 4450 5228 5418 6278
Size: 1666 55 759 1095 254 778 190 860
Cuts at: 6278 621
Size: 1779
Fragments arranged by size:
1779 1666 1095 860 778 759 254 190
55
BsmI GAATG_Cn'
Cuts at: 3545 3545
Size: 7436
BsmBI CGTCTCn'nnnn_
Cuts at: 1430 3837 1430
Size: 2407 5029
Bsp1286I G_dGCh'C
Cuts at: 338 621 1944 2287 2342 2500 2669 2918 3101
Size: 283 1323 343 55 158 169 249 183
Cuts at: 3101 4121 4189 4196 4312 4364 4450 5061 5154
Size: 1020 68 7 116 52 86 611 93
Cuts at: 5154 5228 5418 5480 6278 338
Size: 74 190 62 798 1496
Fragments arranged by size:
1496 1323 1020 798 611 343 283 249
190 183 169 158 116 93 86 74
68 62 55 52 7
BspEI T'CCGG_A
Cuts at: 1476 1476
Size: 7436
BspHI T'CATG_A
Cuts at: 5868 5868
Size: 7436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1449 2264 2277 2613 3049 4486 4700 5002
Size: 569 815 13 336 436 1437 214 302
Cuts at: 5002 5383 880
Size: 381 2933
Fragments arranged by size:
2933 1437 815 569 436 381 336 302
214 13
BsrBI CCG'CTC
Cuts at: 50 275 5728 5782 7266 7431 50
Size: 225 5453 54 1484 165 55
Fragments arranged by size:
5453 1484 225 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1015 2486 2622 2781 5348 1015
Size: 1471 136 159 2567 3103
Fragments arranged by size:
3103 2567 1471 159 136
BsrFI r'CCGG_y
Cuts at: 2566 4084 4199 4224 4234 5434 5615 2566
Size: 1518 115 25 10 1200 181 4387
Fragments arranged by size:
4387 1518 1200 181 115 25 10
BsrGI T'GTAC_A
Cuts at: 935 2067 935
Size: 1132 6304
BssHII G'CGCG_C
Cuts at: 4259 4288 4290 4336 4259
Size: 29 2 46 7359
Fragments arranged by size:
7359 46 29 2
BssSI C'ACGA_G
Cuts at: 5707 6415 5707
Size: 708 6728
BstAPI GCAn_nnn'nTGC
Cuts at: 1821 2256 4813 1821
Size: 435 2557 4444
Fragments arranged by size:
4444 2557 435
BstBI TT'CG_AA
Cuts at: 5797 5797
Size: 7436
BstXI CCAn_nnnn'nTGG
Cuts at: 1166 2826 1166
Size: 1660 5776
BstYI r'GATC_y
Cuts at: 1 1101 2899 2987 4461 4928 4951 5286 5532
Size: 1100 1798 88 1474 467 23 335 246
Cuts at: 5532 5838 5850 5936 6988 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1798 1474 1100 1052 467 449 335 306
246 88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 803 897 947 3089 803
Size: 94 50 2142 5150
Fragments arranged by size:
5150 2142 94 50
BtgI C'CryG_G
Cuts at: 326 2167 2317 2492 3143 326
Size: 1841 150 175 651 4619
Fragments arranged by size:
4619 1841 651 175 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3201 5386 5567 5730 3201
Size: 2185 181 163 4907
Fragments arranged by size:
4907 2185 181 163
BtsI GCAGTG_nn'
Cuts at: 369 2733 3002 4679 369
Size: 2364 269 1677 3126
Fragments arranged by size:
3126 2364 1677 269
ClaI AT'CG_AT
Cuts at: 1178 1178
Size: 7436
DraI TTT'AAA
Cuts at: 4435 4435
Size: 7436
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 7436
DrdI GACnn_nn'nnGTC
Cuts at: 1773 5142 6486 1773
Size: 3369 1344 2723
Fragments arranged by size:
3369 2723 1344
EaeI y'GGCC_r
Cuts at: 356 1798 2240 2514 3003 3975 4039 4065 4303
Size: 1442 442 274 489 972 64 26 238
Cuts at: 4303 4418 5021 5195 5586 5613 6958 356
Size: 115 603 174 391 27 1345 834
Fragments arranged by size:
1442 1345 972 834 603 489 442 391
274 238 174 115 64 27 26
EagI C'GGCC_G
Cuts at: 1798 4039 4303 4418 5021 1798
Size: 2241 264 115 603 4213
Fragments arranged by size:
4213 2241 603 264 115
EarI CTCTTCn'nnn_
Cuts at: 748 806 812 1130 1348 5459 5669 748
Size: 58 6 318 218 4111 210 2515
Fragments arranged by size:
4111 2515 318 218 210 58 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4153 5644 6386 6532 7246 4153
Size: 1491 742 146 714 4343
Fragments arranged by size:
4343 1491 742 714 146
EcoICRI GAG'CTC
Cuts at: 2285 2340 3099 4194 4448 2285
Size: 55 759 1095 254 5273
Fragments arranged by size:
5273 1095 759 254 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 328 466 581 761 976 1013 1115 1676
Size: 105 138 115 180 215 37 102 561
Cuts at: 1676 1694 2418 2810 3229 3734 3807 4285 5261
Size: 18 724 392 419 505 73 478 976
Cuts at: 5261 5693 6046 6708 223
Size: 432 353 662 951
Fragments arranged by size:
976 951 724 662 561 505 478 432
419 392 353 215 180 138 115 105
102 73 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 241 241
Size: 7436
EcoO109I rG'GnC_Cy
Cuts at: 378 555 1317 3759 4297 4360 4361 4575 378
Size: 177 762 2442 538 63 1 214 3239
Fragments arranged by size:
3239 2442 762 538 214 177 63 1
EcoRI G'AATT_C
Cuts at: 4440 4440
Size: 7436
EcoRV GAT'ATC
Cuts at: 3927 3927
Size: 7436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 653 1930 2147 5455 653
Size: 1277 217 3308 2634
Fragments arranged by size:
3308 2634 1277 217
FspI TGC'GCA
Cuts at: 5217 5217
Size: 7436
HaeII r_GCGC'y
Cuts at: 215 3993 4014 4060 4114 4171 4331 4415 4870
Size: 3778 21 46 54 57 160 84 455
Cuts at: 4870 5118 6348 7119 215
Size: 248 1230 771 532
Fragments arranged by size:
3778 1230 771 532 455 248 160 84
57 54 46 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1409 1441 1864 1896 2130 2162 2884 2916 2972
Size: 32 423 32 234 32 722 32 56
Cuts at: 2972 3004 3629 3661 5278 5308 5310 5340 1409
Size: 32 625 32 1617 30 2 30 3505
Fragments arranged by size:
3505 1617 722 625 423 234 56 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 1614 2628 4475 1209
Size: 405 1014 1847 4170
Fragments arranged by size:
4170 1847 1014 405
HindIII A'AGCT_T
Cuts at: 117 117
Size: 7436
KasI G'GCGC_C
Cuts at: 211 4056 4110 4167 4327 4411 4866 5114 211
Size: 3845 54 57 160 84 455 248 2533
Fragments arranged by size:
3845 2533 455 248 160 84 57 54
KpnI G_GTAC'C
Cuts at: 662 4456 662
Size: 3794 3642
MluI A'CGCG_T
Cuts at: 6869 6869
Size: 7436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 3339 3449 3911 6195 6379 272
Size: 3067 110 462 2284 184 1329
Fragments arranged by size:
3067 2284 1329 462 184 110
MscI TGG'CCA
Cuts at: 3005 5197 3005
Size: 2192 5244
MslI CAynn'nnrTG
Cuts at: 158 1562 1568 1736 2491 2582 3019 5425 5552
Size: 1404 6 168 755 91 437 2406 127
Cuts at: 5552 158
Size: 2042
Fragments arranged by size:
2406 2042 1404 755 437 168 127 91
6
MspA1I CmG'CkG
Cuts at: 189 1986 3249 3351 3799 4256 4547 4861 5221
Size: 1797 1263 102 448 457 291 314 360
Cuts at: 5221 6005 6250 189
Size: 784 245 1375
Fragments arranged by size:
1797 1375 1263 784 457 448 360 314
291 245 102
NaeI GCC'GGC
Cuts at: 2568 4086 4201 4226 4236 5617 2568
Size: 1518 115 25 10 1381 4387
Fragments arranged by size:
4387 1518 1381 115 25 10
NarI GG'CG_CC
Cuts at: 212 4057 4111 4168 4328 4412 4867 5115 212
Size: 3845 54 57 160 84 455 248 2533
Fragments arranged by size:
3845 2533 455 248 160 84 57 54
NcoI C'CATG_G
Cuts at: 2167 2492 3143 2167
Size: 325 651 6460
Fragments arranged by size:
6460 651 325
NdeI CA'TA_TG
Cuts at: 1161 2334 1161
Size: 1173 6263
NgoMIV G'CCGG_C
Cuts at: 2566 4084 4199 4224 4234 5615 2566
Size: 1518 115 25 10 1381 4387
Fragments arranged by size:
4387 1518 1381 115 25 10
NsiI A_TGCA'T
Cuts at: 1359 1359
Size: 7436
NspI r_CATG'y
Cuts at: 1284 3024 3688 4489 4989 6592 1284
Size: 1740 664 801 500 1603 2128
Fragments arranged by size:
2128 1740 1603 801 664 500
PasI CC'CwG_GG
Cuts at: 1944 1944
Size: 7436
PciI A'CATG_T
Cuts at: 6588 6588
Size: 7436
PflMI CCAn_nnn'nTGG
Cuts at: 1831 1831
Size: 7436
PfoI T'CCnGG_A
Cuts at: 720 1247 720
Size: 527 6909
PmeI GTTT'AAAC
Cuts at: 4435 4435
Size: 7436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5882 5914 5882
Size: 32 7404
PpuMI rG'GwC_Cy
Cuts at: 378 555 3759 4297 378
Size: 177 3204 538 3517
Fragments arranged by size:
3517 3204 538 177
PshAI GACnn'nnGTC
Cuts at: 431 431
Size: 7436
PsiI TTA'TAA
Cuts at: 2387 2387
Size: 7436
PspOMI G'GGCC_C
Cuts at: 4308 4360 4308
Size: 52 7384
PspXI vC'TCGA_Gb
Cuts at: 4048 4048
Size: 7436
PstI C_TGCA'G
Cuts at: 1316 1460 1491 2816 2955 4483 5168 1316
Size: 144 31 1325 139 1528 685 3584
Fragments arranged by size:
3584 1528 1325 685 144 139 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7436
PvuII CAG'CTG
Cuts at: 189 3249 4861 5221 189
Size: 3060 1612 360 2404
Fragments arranged by size:
3060 2404 1612 360
RsrII CG'GwC_CG
Cuts at: 5631 5631
Size: 7436
SacI G_AGCT'C
Cuts at: 2287 2342 3101 4196 4450 2287
Size: 55 759 1095 254 5273
Fragments arranged by size:
5273 1095 759 254 55
SalI G'TCGA_C
Cuts at: 4473 4473
Size: 7436
SanDI GG'GwC_CC
Cuts at: 4297 4297
Size: 7436
SapI GCTCTTCn'nnn_
Cuts at: 5459 5669 5459
Size: 210 7226
SbfI CC_TGCA'GG
Cuts at: 1316 4483 1316
Size: 3167 4269
ScaI AGT'ACT
Cuts at: 6953 6953
Size: 7436
SexAI A'CCwGG_T
Cuts at: 1616 1616
Size: 7436
SfcI C'TryA_G
Cuts at: 33 1267 1312 1456 1487 1976 2812 2951 3336
Size: 1234 45 144 31 489 836 139 385
Cuts at: 3336 3405 3429 3474 4479 5164 6132 6323 33
Size: 69 24 45 1005 685 968 191 1146
Fragments arranged by size:
1234 1146 1005 968 836 685 489 385
191 144 139 69 45 45 31 24
SfoI GGC'GCC
Cuts at: 213 4058 4112 4169 4329 4413 4868 5116 213
Size: 3845 54 57 160 84 455 248 2533
Fragments arranged by size:
3845 2533 455 248 160 84 57 54
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7436
SgrAI Cr'CCGG_yG
Cuts at: 4224 4224
Size: 7436
SmaI CCC'GGG
Cuts at: 1351 4116 4214 4241 4322 4458 1351
Size: 2765 98 27 81 136 4329
Fragments arranged by size:
4329 2765 136 98 81 27
SmlI C'TyrA_G
Cuts at: 391 2104 3739 4048 4591 5506 6220 6482 391
Size: 1713 1635 309 543 915 714 262 1345
Fragments arranged by size:
1713 1635 1345 915 714 543 309 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7436
SphI G_CATG'C
Cuts at: 4489 4989 4489
Size: 500 6936
SrfI GCCC'GGGC
Cuts at: 4116 4241 4116
Size: 125 7311
StuI AGG'CCT
Cuts at: 825 2308 2889 825
Size: 1483 581 5372
Fragments arranged by size:
5372 1483 581
StyI C'CwwG_G
Cuts at: 1551 2167 2492 3143 3398 4570 4924 1551
Size: 616 325 651 255 1172 354 4063
Fragments arranged by size:
4063 1172 651 616 354 325 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1918 2210 2540 2722 3135 5019 5152 5611 5820
Size: 292 330 182 413 1884 133 459 209
Cuts at: 5820 1918
Size: 3534
Fragments arranged by size:
3534 1884 459 413 330 292 209 182
133
TatI w'GTAC_w
Cuts at: 935 1754 2067 6951 935
Size: 819 313 4884 1420
Fragments arranged by size:
4884 1420 819 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1847 1879 1973 2005 2269 2301 3710 3742 6953
Size: 32 94 32 264 32 1409 32 3211
Cuts at: 6953 6985 1847
Size: 32 2298
Fragments arranged by size:
3211 2298 1409 264 94 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5233 5233
Size: 7436
XbaI T'CTAG_A
Cuts at: 4467 4467
Size: 7436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1294 3917 4931 1294
Size: 2623 1014 3799
Fragments arranged by size:
3799 2623 1014
XhoI C'TCGA_G
Cuts at: 4048 4048
Size: 7436
XmaI C'CCGG_G
Cuts at: 1349 4114 4212 4239 4320 4456 1349
Size: 2765 98 27 81 136 4329
Fragments arranged by size:
4329 2765 136 98 81 27
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AgeI AleI AlfI AloI AscI AvrII BmtI
BsiWI BstEII BstZ17I CspCI FseI FspAI HpaI MfeI
NheI NotI NruI PacI PmlI PsrI SacII SfiI
SnaBI SspI SwaI XmnI ZraI