Restriction map for
pF1KA0706
(Circular) (Six-base) MAPSORT of: pF1KA0706.seq Check: 2683 from: 1 to: 6422
pF1KA0706 6422 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1973 3460 1973
Size: 1487 4935
Acc65I G'GTAC_C
Cuts at: 3438 3438
Size: 6422
AclI AA'CG_TT
Cuts at: 6262 6262
Size: 6422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1297 1870 2368 2807 4247 4679 5426 5694
Size: 375 573 498 439 1440 432 747 268
Cuts at: 5694 922
Size: 1650
Fragments arranged by size:
1650 1440 747 573 498 439 432 375
268
AfeI AGC'GCT
Cuts at: 790 2155 790
Size: 1365 5057
AflIII A'CryG_T
Cuts at: 377 3774 4878 5855 377
Size: 3397 1104 977 944
Fragments arranged by size:
3397 1104 977 944
AgeI A'CCGG_T
Cuts at: 4810 4810
Size: 6422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3398 3398
Size: 6422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1922 1954 1922
Size: 32 6390
AlwNI CAG_nnn'CTG
Cuts at: 648 1251 1261 2055 2115 2688 2961 3727 5294
Size: 603 10 794 60 573 273 766 1567
Cuts at: 5294 648
Size: 1776
Fragments arranged by size:
1776 1567 794 766 603 573 273 60
10
ApaI G_GGCC'C
Cuts at: 634 888 2271 2613 2781 3335 634
Size: 254 1383 342 168 554 3721
Fragments arranged by size:
3721 1383 554 342 254 168
ApaLI G'TGCA_C
Cuts at: 5192 5192
Size: 6422
ApoI r'AATT_y
Cuts at: 1041 3426 5775 6031 1041
Size: 2385 2349 256 1432
Fragments arranged by size:
2385 2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6422
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
AvaI C'yCGr_G
Cuts at: 887 2507 2582 3227 3442 887
Size: 1620 75 645 215 3867
Fragments arranged by size:
3867 1620 645 215 75
BamHI G'GATC_C
Cuts at: 3447 3447
Size: 6422
BanI G'GyrC_C
Cuts at: 115 1303 3438 3852 4100 4135 115
Size: 1188 2135 414 248 35 2402
Fragments arranged by size:
2402 2135 1188 414 248 35
BanII G_rGCy'C
Cuts at: 634 888 1087 2271 2583 2613 2781 2971 3335
Size: 254 199 1184 312 30 168 190 364
Cuts at: 3335 3436 4466 634
Size: 101 1030 2590
Fragments arranged by size:
2590 1184 1030 364 312 254 199 190
168 101 30
BbeI G_GCGC'C
Cuts at: 1307 3856 4104 1307
Size: 2549 248 3625
Fragments arranged by size:
3625 2549 248
BbsI GAAGACnn'nnnn_
Cuts at: 771 1285 1575 1603 1631 2914 771
Size: 514 290 28 28 1283 4279
Fragments arranged by size:
4279 1283 514 290 28 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 344 310
Size: 34 6388
BciVI GTATCCnnnnn_n'
Cuts at: 4320 5081 6422 4320
Size: 761 1341 4320
Fragments arranged by size:
4320 1341 761
BclI T'GATC_A
Cuts at: 3942 3942
Size: 6422
BglI GCCn_nnn'nGGC
Cuts at: 2338 2497 2587 3330 2338
Size: 159 90 743 5430
Fragments arranged by size:
5430 743 159 90
BglII A'GATC_T
Cuts at: 551 2369 3937 5974 551
Size: 1818 1568 2037 999
Fragments arranged by size:
2037 1818 1568 999
BlpI GC'TnA_GC
Cuts at: 515 1463 3534 515
Size: 948 2071 3403
Fragments arranged by size:
3403 2071 948
Bme1580I G_kGCm'C
Cuts at: 467 634 888 2271 2613 2781 3020 3335 4047
Size: 167 254 1383 342 168 239 315 712
Cuts at: 4047 4140 5196 467
Size: 93 1056 1693
Fragments arranged by size:
1693 1383 1056 712 342 315 254 239
168 167 93
BmrI ACTGGGnnnn_n'
Cuts at: 297 2813 3634 3715 4050 6194 297
Size: 2516 821 81 335 2144 525
Fragments arranged by size:
2516 2144 821 525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4789 4789
Size: 6422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1396 1807 2023 2077 2122 2193 2514 1319
Size: 77 411 216 54 45 71 321 5227
Fragments arranged by size:
5227 411 321 216 77 71 54 45
Bpu10I CC'TnA_GC
Cuts at: 1081 1244 1081
Size: 163 6259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 2422 2917 3379 3598 4477 4969 5267 1018
Size: 1404 495 462 219 879 492 298 2173
Fragments arranged by size:
2173 1404 879 495 492 462 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 157 566 736 6126 157
Size: 409 170 5390 453
Fragments arranged by size:
5390 453 409 170
BsaAI yAC'GTr
Cuts at: 639 3775 639
Size: 3136 3286
BsaBI GATnn'nnATC
Cuts at: 6 3936 5973 6
Size: 3930 2037 455
Fragments arranged by size:
3930 2037 455
BsaHI Gr'CG_yC
Cuts at: 1304 1580 1670 2489 3853 4101 4803 6299 1304
Size: 276 90 819 1364 248 702 1496 1427
Fragments arranged by size:
1496 1427 1364 819 702 276 248 90
BsaWI w'CCGG_w
Cuts at: 1711 1745 2069 3611 4132 4810 5084 5231 5642
Size: 34 324 1542 521 678 274 147 411
Cuts at: 5642 1711
Size: 2491
Fragments arranged by size:
2491 1542 678 521 411 324 274 147
34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 1760 1790 2050 2080 2863 2893 196
Size: 30 1534 30 260 30 783 30 3725
Fragments arranged by size:
3725 1534 783 260 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 867 1120 1498 2175 2548 2587 2821 2908 3142
Size: 253 378 677 373 39 234 87 234
Cuts at: 3142 867
Size: 4147
Fragments arranged by size:
4147 677 378 373 253 234 234 87
39
BseYI C'CCAG_C
Cuts at: 409 467 856 1329 1757 2248 2294 2327 2867
Size: 58 389 473 428 491 46 33 540
Cuts at: 2867 3003 3062 3257 3293 3312 3317 3847 4605
Size: 136 59 195 36 19 5 530 758
Cuts at: 4605 5182 5678 409
Size: 577 496 1153
Fragments arranged by size:
1153 758 577 540 530 496 491 473
428 389 195 136 59 58 46 36
33 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 3227 661
Size: 2566 3856
BsiEI CG_ry'CG
Cuts at: 104 3964 4010 5218 104
Size: 3860 46 1208 1308
Fragments arranged by size:
3860 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 516 620 1022 3436 4214 4404 5196 516
Size: 104 402 2414 778 190 792 1742
Fragments arranged by size:
2414 1742 792 778 402 190 104
BsiWI C'GTAC_G
Cuts at: 635 635
Size: 6422
BsmBI CGTCTCn'nnnn_
Cuts at: 2480 3363 2480
Size: 883 5539
Bsp1286I G_dGCh'C
Cuts at: 467 516 620 634 888 1022 1087 2271 2583
Size: 49 104 14 254 134 65 1184 312
Cuts at: 2583 2613 2781 2971 3020 3335 3436 4047 4140
Size: 30 168 190 49 315 101 611 93
Cuts at: 4140 4214 4404 4466 5196 467
Size: 74 190 62 730 1693
Fragments arranged by size:
1693 1184 730 611 315 312 254 190
190 168 134 104 101 93 74 65
62 49 49 30 14
BspEI T'CCGG_A
Cuts at: 1745 5642 1745
Size: 3897 2525
BspHI T'CATG_A
Cuts at: 2739 5598 2739
Size: 2859 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3472 3686 3988 4369 3472
Size: 214 302 381 5525
Fragments arranged by size:
5525 381 302 214
BsrBI CCG'CTC
Cuts at: 50 564 2874 3095 3114 3235 4714 4768 6252
Size: 514 2310 221 19 121 1479 54 1484
Cuts at: 6252 6417 50
Size: 165 55
Fragments arranged by size:
2310 1484 1479 514 221 165 121 55
54 19
BsrDI GCAATG_nn'
Cuts at: 680 1164 1528 4334 680
Size: 484 364 2806 2768
Fragments arranged by size:
2806 2768 484 364
BsrFI r'CCGG_y
Cuts at: 3161 4420 4601 4810 3161
Size: 1259 181 209 4773
Fragments arranged by size:
4773 1259 209 181
BssSI C'ACGA_G
Cuts at: 4693 5051 4693
Size: 358 6064
BstAPI GCAn_nnn'nTGC
Cuts at: 682 2688 2961 3799 682
Size: 2006 273 838 3305
Fragments arranged by size:
3305 2006 838 273
BstBI TT'CG_AA
Cuts at: 4783 4783
Size: 6422
BstXI CCAn_nnnn'nTGG
Cuts at: 186 1765 1976 186
Size: 1579 211 4632
Fragments arranged by size:
4632 1579 211
BstYI r'GATC_y
Cuts at: 1 551 2052 2288 2369 3447 3914 3937 4272
Size: 550 1501 236 81 1078 467 23 335
Cuts at: 4272 4518 5530 5616 5628 5974 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1501 1078 1012 550 467 449 346 335
246 236 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 879 1123 1418 1510 3125 879
Size: 244 295 92 1615 4176
Fragments arranged by size:
4176 1615 295 244 92
BtgI C'CryG_G
Cuts at: 107 1427 2213 2336 2403 107
Size: 1320 786 123 67 4126
Fragments arranged by size:
4126 1320 786 123 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4372 4553 4716 4372
Size: 181 163 6078
Fragments arranged by size:
6078 181 163
BtsI GCAGTG_nn'
Cuts at: 140 2259 2562 2574 3665 140
Size: 2119 303 12 1091 2897
Fragments arranged by size:
2897 2119 1091 303 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2220 2255 3384 3419 2220
Size: 35 1129 35 5223
Fragments arranged by size:
5223 1129 35 35
DraI TTT'AAA
Cuts at: 3421 3421
Size: 6422
DraIII CAC_nnn'GTG
Cuts at: 1611 1611
Size: 6422
DrdI GACnn_nn'nnGTC
Cuts at: 4128 4986 4128
Size: 858 5564
EaeI y'GGCC_r
Cuts at: 1550 1683 2429 3079 4007 4181 4572 4599 5944
Size: 133 746 650 928 174 391 27 1345
Cuts at: 5944 1550
Size: 2028
Fragments arranged by size:
2028 1345 928 746 650 391 174 133
27
EagI C'GGCC_G
Cuts at: 4007 4007
Size: 6422
EarI CTCTTCn'nnn_
Cuts at: 338 490 2035 2159 4445 4655 338
Size: 152 1545 124 2286 210 2105
Fragments arranged by size:
2286 2105 1545 210 152 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2389 2605 4630 4940 5086 6232 2389
Size: 216 2025 310 146 1146 2579
Fragments arranged by size:
2579 2025 1146 310 216 146
EcoICRI GAG'CTC
Cuts at: 3434 3434
Size: 6422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1297 1319 1396 1807 1870 2023 2077 2122
Size: 375 22 77 411 63 153 54 45
Cuts at: 2122 2193 2368 2514 2807 4247 4679 5426 5694
Size: 71 175 146 293 1440 432 747 268
Cuts at: 5694 922
Size: 1650
Fragments arranged by size:
1650 1440 747 432 411 375 293 268
175 153 146 77 71 63 54 45
22
EcoNI CCTnn'n_nnAGG
Cuts at: 1796 2731 1796
Size: 935 5487
EcoO109I rG'GnC_Cy
Cuts at: 297 480 645 884 1176 1938 2267 2268 2424
Size: 183 165 239 292 762 329 1 156
Cuts at: 2424 2601 2685 2769 2778 2898 2999 3182 3331
Size: 177 84 84 9 120 101 183 149
Cuts at: 3331 3332 3561 297
Size: 1 229 3158
Fragments arranged by size:
3158 762 329 292 239 229 183 183
177 165 156 149 120 101 84 84
9 1 1
EcoRI G'AATT_C
Cuts at: 3426 3426
Size: 6422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 3057 4441 2110
Size: 947 1384 4091
Fragments arranged by size:
4091 1384 947
FspI TGC'GCA
Cuts at: 2995 4203 2995
Size: 1208 5214
HaeII r_GCGC'y
Cuts at: 792 1307 2157 3856 4104 5126 6105 792
Size: 515 850 1699 248 1022 979 1109
Fragments arranged by size:
1699 1109 1022 979 850 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 902 934 958 990 2140 2172 2863
Size: 32 398 32 24 32 1150 32 691
Cuts at: 2863 2895 4264 4294 4296 4326 472
Size: 32 1369 30 2 30 2568
Fragments arranged by size:
2568 1369 1150 691 398 32 32 32
32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 1974 3461 1974
Size: 1487 4935
HindIII A'AGCT_T
Cuts at: 252 1825 252
Size: 1573 4849
KasI G'GCGC_C
Cuts at: 1303 3852 4100 1303
Size: 2549 248 3625
Fragments arranged by size:
3625 2549 248
KpnI G_GTAC'C
Cuts at: 3442 3442
Size: 6422
MluI A'CGCG_T
Cuts at: 5855 5855
Size: 6422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 3130 3141 5093 5277 971
Size: 2159 11 1952 184 2116
Fragments arranged by size:
2159 2116 1952 184 11
MscI TGG'CCA
Cuts at: 3081 4183 3081
Size: 1102 5320
MslI CAynn'nnrTG
Cuts at: 733 1066 2744 4411 4538 733
Size: 333 1678 1667 127 2617
Fragments arranged by size:
2617 1678 1667 333 127
MspA1I CmG'CkG
Cuts at: 1154 1315 1842 2135 2202 2327 3321 3412 3533
Size: 161 527 293 67 125 994 91 121
Cuts at: 3533 3847 4207 5220 5465 1154
Size: 314 360 1013 245 2111
Fragments arranged by size:
2111 1013 994 527 360 314 293 245
161 125 121 91 67
NaeI GCC'GGC
Cuts at: 3163 4603 3163
Size: 1440 4982
NarI GG'CG_CC
Cuts at: 1304 3853 4101 1304
Size: 2549 248 3625
Fragments arranged by size:
3625 2549 248
NcoI C'CATG_G
Cuts at: 107 1427 2336 107
Size: 1320 909 4193
Fragments arranged by size:
4193 1320 909
NdeI CA'TA_TG
Cuts at: 1812 1812
Size: 6422
NgoMIV G'CCGG_C
Cuts at: 3161 4601 3161
Size: 1440 4982
NspI r_CATG'y
Cuts at: 198 897 2703 2832 3475 3975 4882 198
Size: 699 1806 129 643 500 907 1738
Fragments arranged by size:
1806 1738 907 699 643 500 129
PasI CC'CwG_GG
Cuts at: 2774 3328 2774
Size: 554 5868
PciI A'CATG_T
Cuts at: 4878 4878
Size: 6422
PfoI T'CCnGG_A
Cuts at: 611 1556 611
Size: 945 5477
PmeI GTTT'AAAC
Cuts at: 3421 3421
Size: 6422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 582 614 5561 5593 582
Size: 32 4947 32 1411
Fragments arranged by size:
4947 1411 32 32
PpuMI rG'GwC_Cy
Cuts at: 297 480 645 1176 1938 2601 2769 2898 3182
Size: 183 165 531 762 663 168 129 284
Cuts at: 3182 297
Size: 3537
Fragments arranged by size:
3537 762 663 531 284 183 168 165
129
PshAI GACnn'nnGTC
Cuts at: 2227 2227
Size: 6422
PspOMI G'GGCC_C
Cuts at: 630 884 2267 2609 2777 3331 630
Size: 254 1383 342 168 554 3721
Fragments arranged by size:
3721 1383 554 342 254 168
PstI C_TGCA'G
Cuts at: 1208 1442 2051 2312 2642 2657 2684 2957 2966
Size: 234 609 261 330 15 27 273 9
Cuts at: 2966 3469 4154 1208
Size: 503 685 3476
Fragments arranged by size:
3476 685 609 503 330 273 261 234
27 15 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6422
PvuII CAG'CTG
Cuts at: 1315 2202 3412 3847 4207 1315
Size: 887 1210 435 360 3530
Fragments arranged by size:
3530 1210 887 435 360
RsrII CG'GwC_CG
Cuts at: 4617 4617
Size: 6422
SacI G_AGCT'C
Cuts at: 3436 3436
Size: 6422
SalI G'TCGA_C
Cuts at: 1972 3459 1972
Size: 1487 4935
SanDI GG'GwC_CC
Cuts at: 1938 1938
Size: 6422
SapI GCTCTTCn'nnn_
Cuts at: 4445 4655 4445
Size: 210 6212
SbfI CC_TGCA'GG
Cuts at: 3469 3469
Size: 6422
ScaI AGT'ACT
Cuts at: 5939 5939
Size: 6422
SexAI A'CCwGG_T
Cuts at: 1607 1607
Size: 6422
SfcI C'TryA_G
Cuts at: 33 1005 1204 1438 2047 2308 2525 2638 2653
Size: 972 199 234 609 261 217 113 15
Cuts at: 2653 2680 2953 2962 3465 4150 5143 5334 33
Size: 27 273 9 503 685 993 191 1121
Fragments arranged by size:
1121 993 972 685 609 503 273 261
234 217 199 191 113 27 15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3330 3330
Size: 6422
SfoI GGC'GCC
Cuts at: 1305 3854 4102 1305
Size: 2549 248 3625
Fragments arranged by size:
3625 2549 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6422
SmaI CCC'GGG
Cuts at: 889 2509 3229 3444 889
Size: 1620 720 215 3867
Fragments arranged by size:
3867 1620 720 215
SmlI C'TyrA_G
Cuts at: 1033 2437 2932 3394 3577 4492 4984 5246 1033
Size: 1404 495 462 183 915 492 262 2209
Fragments arranged by size:
2209 1404 915 495 492 462 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6422
SphI G_CATG'C
Cuts at: 198 897 2703 2832 3475 3975 198
Size: 699 1806 129 643 500 2645
Fragments arranged by size:
2645 1806 699 643 500 129
StuI AGG'CCT
Cuts at: 1274 1274
Size: 6422
StyI C'CwwG_G
Cuts at: 107 1427 2336 3556 3910 107
Size: 1320 909 1220 354 2619
Fragments arranged by size:
2619 1320 1220 909 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 4005 4138 4597 4806 1368
Size: 2637 133 459 209 2984
Fragments arranged by size:
2984 2637 459 209 133
TatI w'GTAC_w
Cuts at: 5937 5937
Size: 6422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 166 198 1760 1792 3126 3158 5939 5971 166
Size: 32 1562 32 1334 32 2781 32 617
Fragments arranged by size:
2781 1562 1334 617 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4219 4219
Size: 6422
XbaI T'CTAG_A
Cuts at: 3453 3453
Size: 6422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1663 2864 3917 1663
Size: 1201 1053 4168
Fragments arranged by size:
4168 1201 1053
XmaI C'CCGG_G
Cuts at: 887 2507 3227 3442 887
Size: 1620 720 215 3867
Fragments arranged by size:
3867 1620 720 215
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BsmI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PmlI PsiI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI