Restriction map for
pF1KA0708
(Circular) (Six-base) MAPSORT of: pF1KA0708.seq Check: 5309 from: 1 to: 10664
pF1KA0708 10664 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6457 6655 6457
Size: 198 10466
AccI GT'mk_AC
Cuts at: 323 1372 1855 6737 7702 323
Size: 1049 483 4882 965 3285
Fragments arranged by size:
4882 3285 1049 965 483
Acc65I G'GTAC_C
Cuts at: 7680 7680
Size: 10664
AclI AA'CG_TT
Cuts at: 10504 10504
Size: 10664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 262 358 576 836 1433 2521 4431 4813
Size: 68 96 218 260 597 1088 1910 382
Cuts at: 4813 5509 7393 8489 8921 9668 9936 194
Size: 696 1884 1096 432 747 268 922
Fragments arranged by size:
1910 1884 1096 1088 922 747 696 597
432 382 268 260 218 96 68
AfeI AGC'GCT
Cuts at: 892 1204 3946 6811 892
Size: 312 2742 2865 4745
Fragments arranged by size:
4745 2865 2742 312
AflIII A'CryG_T
Cuts at: 626 2792 4048 8016 9120 10097 626
Size: 2166 1256 3968 1104 977 1193
Fragments arranged by size:
3968 2166 1256 1193 1104 977
AgeI A'CCGG_T
Cuts at: 9052 9052
Size: 10664
AhdI GACnn_n'nnGTC
Cuts at: 6137 6137
Size: 10664
AleI CACnn'nnGTG
Cuts at: 3740 4742 5594 3740
Size: 1002 852 8810
Fragments arranged by size:
8810 1002 852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 691 865 4181 7253 7354 7400 691
Size: 174 3316 3072 101 46 3955
Fragments arranged by size:
3955 3316 3072 174 101 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 994
Size: 32 10632
AlwNI CAG_nnn'CTG
Cuts at: 471 684 1456 2043 2361 3276 3393 4206 5016
Size: 213 772 587 318 915 117 813 810
Cuts at: 5016 5412 5952 6052 6222 6630 7122 7969 9536
Size: 396 540 100 170 408 492 847 1567
Cuts at: 9536 471
Size: 1599
Fragments arranged by size:
1599 1567 915 847 813 810 772 587
540 492 408 396 318 213 170 117
100
ApaI G_GGCC'C
Cuts at: 578 1068 1423 2983 3128 4000 4590 5624 5984
Size: 490 355 1560 145 872 590 1034 360
Cuts at: 5984 578
Size: 5258
Fragments arranged by size:
5258 1560 1034 872 590 490 360 355
145
ApaLI G'TGCA_C
Cuts at: 1393 6585 7005 9434 1393
Size: 5192 420 2429 2623
Fragments arranged by size:
5192 2623 2429 420
ApoI r'AATT_y
Cuts at: 1210 4161 5275 5449 7668 10017 10273 1210
Size: 2951 1114 174 2219 2349 256 1601
Fragments arranged by size:
2951 2349 2219 1601 1114 256 174
AseI AT'TA_AT
Cuts at: 20 20
Size: 10664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10664
AvaI C'yCGr_G
Cuts at: 410 2628 3971 4367 4661 7528 7684 410
Size: 2218 1343 396 294 2867 156 3390
Fragments arranged by size:
3390 2867 2218 1343 396 294 156
AvrII C'CTAG_G
Cuts at: 1707 1707
Size: 10664
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1860 1893 1860
Size: 33 10631
BamHI G'GATC_C
Cuts at: 7349 7689 7349
Size: 340 10324
BanI G'GyrC_C
Cuts at: 141 3043 4109 4482 4702 4835 6851 7680 8094
Size: 2902 1066 373 220 133 2016 829 414
Cuts at: 8094 8342 8377 141
Size: 248 35 2428
Fragments arranged by size:
2902 2428 2016 1066 829 414 373 248
220 133 35
BanII G_rGCy'C
Cuts at: 578 609 714 1068 1423 1982 2213 2635 2983
Size: 31 105 354 355 559 231 422 348
Cuts at: 2983 3128 3224 3586 3854 4000 4480 4590 4803
Size: 145 96 362 268 146 480 110 213
Cuts at: 4803 5624 5975 5984 6103 6334 7678 8708 578
Size: 821 351 9 119 231 1344 1030 2534
Fragments arranged by size:
2534 1344 1030 821 559 480 422 362
355 354 351 348 268 231 231 213
146 145 119 110 105 96 31 9
BbeI G_GCGC'C
Cuts at: 4113 4486 4706 8098 8346 4113
Size: 373 220 3392 248 6431
Fragments arranged by size:
6431 3392 373 248 220
BbsI GAAGACnn'nnnn_
Cuts at: 582 1185 3518 5722 7399 7514 582
Size: 603 2333 2204 1677 115 3732
Fragments arranged by size:
3732 2333 2204 1677 603 115
BbvCI CC'TCA_GC
Cuts at: 1075 3154 7321 1075
Size: 2079 4167 4418
Fragments arranged by size:
4418 4167 2079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4021 4055 4021
Size: 34 10630
BciVI GTATCCnnnnn_n'
Cuts at: 8562 9323 10664 8562
Size: 761 1341 8562
Fragments arranged by size:
8562 1341 761
BclI T'GATC_A
Cuts at: 5778 8184 5778
Size: 2406 8258
BfrBI ATG'CAT
Cuts at: 3309 3309
Size: 10664
BglI GCCn_nnn'nGGC
Cuts at: 150 1226 3029 3242 3956 4201 5979 6626 7435
Size: 1076 1803 213 714 245 1778 647 809
Cuts at: 7435 7533 150
Size: 98 3281
Fragments arranged by size:
3281 1803 1778 1076 809 714 647 245
213 98
BglII A'GATC_T
Cuts at: 1715 1808 2597 8179 10216 1715
Size: 93 789 5582 2037 2163
Fragments arranged by size:
5582 2163 2037 789 93
BlpI GC'TnA_GC
Cuts at: 3386 4537 5387 6179 7776 3386
Size: 1151 850 792 1597 6274
Fragments arranged by size:
6274 1597 1151 850 792
Bme1580I G_kGCm'C
Cuts at: 146 360 578 1068 1397 1423 1492 2983 3128
Size: 214 218 490 329 26 69 1491 145
Cuts at: 3128 4000 4074 4287 4590 5537 5624 5984 6589
Size: 872 74 213 303 947 87 360 605
Cuts at: 6589 7009 7079 7529 8289 8382 9438 146
Size: 420 70 450 760 93 1056 1372
Fragments arranged by size:
1491 1372 1056 947 872 760 605 490
450 420 360 329 303 218 214 213
145 93 87 74 70 69 26
BmgBI CAC'GTC
Cuts at: 717 908 5372 717
Size: 191 4464 6009
Fragments arranged by size:
6009 4464 191
BmrI ACTGGGnnnn_n'
Cuts at: 1398 3696 4216 4521 6213 7876 7957 8292 10436
Size: 2298 520 305 1692 1663 81 335 2144
Cuts at: 10436 1398
Size: 1626
Fragments arranged by size:
2298 2144 1692 1663 1626 520 335 305
81
BmtI G_CTAG'C
Cuts at: 6713 6713
Size: 10664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 728 2039 2712 3375 3651 6540 6585 9031 728
Size: 1311 673 663 276 2889 45 2446 2361
Fragments arranged by size:
2889 2446 2361 1311 673 663 276 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 971 1027 1200 1240 1429 1534 3055 3450
Size: 163 56 173 40 189 105 1521 395
Cuts at: 3450 3570 4101 4102 4826 4966 5047 5286 5506
Size: 120 531 1 724 140 81 239 220
Cuts at: 5506 5821 5951 7144 7243 7276 808
Size: 315 130 1193 99 33 4196
Fragments arranged by size:
4196 1521 1193 724 531 395 315 239
220 189 173 163 140 130 120 105
99 81 56 40 33 1
Bpu10I CC'TnA_GC
Cuts at: 641 1075 2900 2951 3154 4001 4427 4924 5017
Size: 434 1825 51 203 847 426 497 93
Cuts at: 5017 6245 6287 7321 641
Size: 1228 42 1034 3984
Fragments arranged by size:
3984 1825 1228 1034 847 497 434 426
203 93 51 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3046 3771 4362 5742 6889 7840 8719 9211 9509
Size: 725 591 1380 1147 951 879 492 298
Cuts at: 9509 3046
Size: 4201
Fragments arranged by size:
4201 1380 1147 951 879 725 591 492
298
BsaI GGTCTCn'nnnn_
Cuts at: 947 1681 1993 2756 2911 3073 4318 5473 7274
Size: 734 312 763 155 162 1245 1155 1801
Cuts at: 7274 10368 947
Size: 3094 1243
Fragments arranged by size:
3094 1801 1245 1243 1155 763 734 312
162 155
BsaAI yAC'GTr
Cuts at: 549 5922 8017 549
Size: 5373 2095 3196
Fragments arranged by size:
5373 3196 2095
BsaBI GATnn'nnATC
Cuts at: 6 8178 10215 6
Size: 8172 2037 455
Fragments arranged by size:
8172 2037 455
BsaHI Gr'CG_yC
Cuts at: 4110 4483 4703 8095 8343 9045 10541 4110
Size: 373 220 3392 248 702 1496 4233
Fragments arranged by size:
4233 3392 1496 702 373 248 220
BsaWI w'CCGG_w
Cuts at: 4472 6481 7853 8374 9052 9326 9473 9884 4472
Size: 2009 1372 521 678 274 147 411 5252
Fragments arranged by size:
5252 2009 1372 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 946 976 1226 1256 1888 1918 3032 3062 3448
Size: 30 250 30 632 30 1114 30 386
Cuts at: 3448 3478 3595 3625 4021 4051 5284 5314 5629
Size: 30 117 30 396 30 1233 30 315
Cuts at: 5629 5659 6967 6997 7273 7303 7561 7591 946
Size: 30 1308 30 276 30 258 30 4019
Fragments arranged by size:
4019 1308 1233 1114 632 396 386 315
276 258 250 117 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1309 1909 2251 2514 2638 3049 4105 4400 5044
Size: 600 342 263 124 411 1056 295 644
Cuts at: 5044 5116 5140 5143 5152 5479 6498 7281 7467
Size: 72 24 3 9 327 1019 783 186
Cuts at: 7467 7513 7516 7570 1309
Size: 46 3 54 4403
Fragments arranged by size:
4403 1056 1019 783 644 600 411 342
327 295 263 186 124 72 54 46
24 9 3 3
BseYI C'CCAG_C
Cuts at: 127 388 700 942 1061 1141 1306 2485 3083
Size: 261 312 242 119 80 165 1179 598
Cuts at: 3083 3396 3498 3614 4220 4818 5376 5624 5854
Size: 313 102 116 606 598 558 248 230
Cuts at: 5854 6040 6265 6473 6662 6817 8089 8847 9424
Size: 186 225 208 189 155 1272 758 577
Cuts at: 9424 9920 127
Size: 496 871
Fragments arranged by size:
1272 1179 871 758 606 598 598 577
558 496 313 312 261 248 242 230
225 208 189 186 165 155 119 116
102 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 1264 1375 2191 2374 2497 2927 3712 4267
Size: 581 111 816 183 123 430 785 555
Cuts at: 4267 4567 5449 5884 6166 6841 7213 7296 683
Size: 300 882 435 282 675 372 83 4051
Fragments arranged by size:
4051 882 816 785 675 581 555 435
430 372 300 282 183 123 111 83
BsiEI CG_ry'CG
Cuts at: 104 527 2159 2404 6609 8206 8252 9460 104
Size: 423 1632 245 4205 1597 46 1208 1308
Fragments arranged by size:
4205 1632 1597 1308 1208 423 245 46
BsiHKAI G_wGCw'C
Cuts at: 293 554 1397 2698 3332 3854 4628 4892 5023
Size: 261 843 1301 634 522 774 264 131
Cuts at: 5023 6589 7009 7678 8456 8646 9438 293
Size: 1566 420 669 778 190 792 1519
Fragments arranged by size:
1566 1519 1301 843 792 778 774 669
634 522 420 264 261 190 131
BsiWI C'GTAC_G
Cuts at: 6300 6300
Size: 10664
BsmI GAATG_Cn'
Cuts at: 842 1343 3485 7380 842
Size: 501 2142 3895 4126
Fragments arranged by size:
4126 3895 2142 501
BsmBI CGTCTCn'nnnn_
Cuts at: 4924 5232 6145 6510 4924
Size: 308 913 365 9078
Fragments arranged by size:
9078 913 365 308
Bsp1286I G_dGCh'C
Cuts at: 146 293 360 554 578 609 714 1068 1397
Size: 147 67 194 24 31 105 354 329
Cuts at: 1397 1423 1492 1982 2213 2635 2698 2983 3128
Size: 26 69 490 231 422 63 285 145
Cuts at: 3128 3224 3332 3586 3854 4000 4074 4287 4480
Size: 96 108 254 268 146 74 213 193
Cuts at: 4480 4590 4628 4803 4892 5023 5537 5624 5975
Size: 110 38 175 89 131 514 87 351
Cuts at: 5975 5984 6103 6334 6589 7009 7079 7529 7678
Size: 9 119 231 255 420 70 450 149
Cuts at: 7678 8289 8382 8456 8646 8708 9438 146
Size: 611 93 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 514 490 450 422 420
354 351 329 285 268 255 254 231
231 213 194 193 190 175 149 147
146 145 131 119 110 108 105 96
93 89 87 74 74 70 69 67
63 62 38 31 26 24 9
BspEI T'CCGG_A
Cuts at: 9884 9884
Size: 10664
BspHI T'CATG_A
Cuts at: 2381 2858 5610 9840 2381
Size: 477 2752 4230 3205
Fragments arranged by size:
4230 3205 2752 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 623 1347 1743 1802 2204 2384 3289 4225 4545
Size: 724 396 59 402 180 905 936 320
Cuts at: 4545 6362 6457 6655 7714 7928 8230 8611 623
Size: 1817 95 198 1059 214 302 381 2676
Fragments arranged by size:
2676 1817 1059 936 905 724 402 396
381 320 302 214 198 180 95 59
BsrBI CCG'CTC
Cuts at: 50 354 2250 2406 5965 8956 9010 10494 10659
Size: 304 1896 156 3559 2991 54 1484 165
Cuts at: 10659 50
Size: 55
Fragments arranged by size:
3559 2991 1896 1484 304 165 156 55
54
BsrDI GCAATG_nn'
Cuts at: 446 781 3197 6455 8576 446
Size: 335 2416 3258 2121 2534
Fragments arranged by size:
3258 2534 2416 2121 335
BsrFI r'CCGG_y
Cuts at: 3478 3706 4106 5939 8662 8843 9052 3478
Size: 228 400 1833 2723 181 209 5090
Fragments arranged by size:
5090 2723 1833 400 228 209 181
BsrGI T'GTAC_A
Cuts at: 7160 7160
Size: 10664
BssSI C'ACGA_G
Cuts at: 2392 3208 4153 5530 8935 9293 2392
Size: 816 945 1377 3405 358 3763
Fragments arranged by size:
3763 3405 1377 945 816 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2361 3836 4217 5412 6135 6617 8041 2361
Size: 1475 381 1195 723 482 1424 4984
Fragments arranged by size:
4984 1475 1424 1195 723 482 381
BstBI TT'CG_AA
Cuts at: 9025 9025
Size: 10664
BstXI CCAn_nnnn'nTGG
Cuts at: 495 700 742 2097 2331 3369 3474 3810 5219
Size: 205 42 1355 234 1038 105 336 1409
Cuts at: 5219 5632 495
Size: 413 5527
Fragments arranged by size:
5527 1409 1355 1038 413 336 234 205
105 42
BstYI r'GATC_y
Cuts at: 1 414 650 857 1412 1715 1808 1884 2531
Size: 413 236 207 555 303 93 76 647
Cuts at: 2531 2597 3353 3932 4775 5175 5948 7087 7247
Size: 66 756 579 843 400 773 1139 160
Cuts at: 7247 7349 7689 8156 8179 8514 8760 9772 9858
Size: 102 340 467 23 335 246 1012 86
Cuts at: 9858 9870 10216 1
Size: 12 346 449
Fragments arranged by size:
1139 1012 843 773 756 647 579 555
467 449 413 400 346 340 335 303
246 236 207 160 102 93 86 76
66 23 12
Bsu36I CC'TnA_GG
Cuts at: 147 317 1424 3185 3565 4513 5544 6692 7479
Size: 170 1107 1761 380 948 1031 1148 787
Cuts at: 7479 147
Size: 3332
Fragments arranged by size:
3332 1761 1148 1107 1031 948 787 380
170
BtgI C'CryG_G
Cuts at: 107 1727 2006 3738 5754 5975 6882 6950 107
Size: 1620 279 1732 2016 221 907 68 3821
Fragments arranged by size:
3821 2016 1732 1620 907 279 221 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3277 8614 8795 8958 3277
Size: 5337 181 163 4983
Fragments arranged by size:
5337 4983 181 163
BtsI GCAGTG_nn'
Cuts at: 822 3258 4695 4727 5371 5637 6250 6312 7907
Size: 2436 1437 32 644 266 613 62 1595
Cuts at: 7907 822
Size: 3579
Fragments arranged by size:
3579 2436 1595 1437 644 613 266 62
32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4866 4901 4866
Size: 35 10629
DraI TTT'AAA
Cuts at: 7663 7663
Size: 10664
DraIII CAC_nnn'GTG
Cuts at: 2017 2017
Size: 10664
DrdI GACnn_nn'nnGTC
Cuts at: 8370 9228 8370
Size: 858 9806
EaeI y'GGCC_r
Cuts at: 485 524 1102 1736 2099 2162 2401 2510 2550
Size: 39 578 634 363 63 239 109 40
Cuts at: 2550 3476 3773 4015 4874 6717 6791 8249 8423
Size: 926 297 242 859 1843 74 1458 174
Cuts at: 8423 8814 8841 10186 485
Size: 391 27 1345 963
Fragments arranged by size:
1843 1458 1345 963 926 859 634 578
391 363 297 242 239 174 109 74
63 40 39 27
EagI C'GGCC_G
Cuts at: 524 2401 8249 524
Size: 1877 5848 2939
Fragments arranged by size:
5848 2939 1877
EarI CTCTTCn'nnn_
Cuts at: 1886 1922 2703 4060 5059 5117 5129 5138 5704
Size: 36 781 1357 999 58 12 9 566
Cuts at: 5704 7490 7628 8687 8897 1886
Size: 1786 138 1059 210 3653
Fragments arranged by size:
3653 1786 1357 1059 999 781 566 210
138 58 36 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 643 1186 4927 7362 8872 9182 9328 10474 643
Size: 543 3741 2435 1510 310 146 1146 833
Fragments arranged by size:
3741 2435 1510 1146 833 543 310 146
EcoICRI GAG'CTC
Cuts at: 3852 7676 3852
Size: 3824 6840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 262 358 576 808 836 971 1027 1200
Size: 68 96 218 232 28 135 56 173
Cuts at: 1200 1240 1429 1433 1534 2521 3055 3450 3570
Size: 40 189 4 101 987 534 395 120
Cuts at: 3570 4101 4102 4431 4813 4826 4966 5047 5286
Size: 531 1 329 382 13 140 81 239
Cuts at: 5286 5506 5509 5821 5951 7144 7243 7276 7393
Size: 220 3 312 130 1193 99 33 117
Cuts at: 7393 8489 8921 9668 9936 194
Size: 1096 432 747 268 922
Fragments arranged by size:
1193 1096 987 922 747 534 531 432
395 382 329 312 268 239 232 220
218 189 173 140 135 130 120 117
101 99 96 81 68 56 40 33
28 13 4 3 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2224 3073 5569 7255 2224
Size: 849 2496 1686 5633
Fragments arranged by size:
5633 2496 1686 849
EcoO109I rG'GnC_Cy
Cuts at: 132 151 509 575 1138 1420 1704 1959 2116
Size: 19 358 66 563 282 284 255 157
Cuts at: 2116 2358 2945 2979 3053 3124 3414 3990 3997
Size: 242 587 34 74 71 290 576 7
Cuts at: 3997 4227 4586 4587 5621 5640 5766 5980 5981
Size: 230 359 1 1034 19 126 214 1
Cuts at: 5981 6240 6627 7444 7803 132
Size: 259 387 817 359 2993
Fragments arranged by size:
2993 1034 817 587 576 563 387 359
359 358 290 284 282 259 255 242
230 214 157 126 74 71 66 34
19 19 7 1 1
EcoRI G'AATT_C
Cuts at: 1210 5275 7668 1210
Size: 4065 2393 4206
Fragments arranged by size:
4206 4065 2393
EcoRV GAT'ATC
Cuts at: 1585 2177 1585
Size: 592 10072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1653 5100 8683 1653
Size: 3447 3583 3634
Fragments arranged by size:
3634 3583 3447
FspI TGC'GCA
Cuts at: 4687 8445 4687
Size: 3758 6906
HaeII r_GCGC'y
Cuts at: 472 894 1206 3069 3948 4113 4486 4706 5043
Size: 422 312 1863 879 165 373 220 337
Cuts at: 5043 6212 6544 6813 6900 8098 8346 9368 10347
Size: 1169 332 269 87 1198 248 1022 979
Cuts at: 10347 472
Size: 789
Fragments arranged by size:
1863 1198 1169 1022 979 879 789 422
373 337 332 312 269 248 220 165
87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 946 978 1888 1920 1921 1953 2546
Size: 32 693 32 910 32 1 32 593
Cuts at: 2546 2578 3364 3396 5284 5316 5455 5487 5872
Size: 32 786 32 1888 32 139 32 385
Cuts at: 5872 5904 6123 6155 6343 6375 7273 7305 7561
Size: 32 219 32 188 32 898 32 256
Cuts at: 7561 7593 8506 8536 8538 8568 221
Size: 32 913 30 2 30 2317
Fragments arranged by size:
2317 1888 913 910 898 786 693 593
385 256 219 188 139 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 1373 6738 7703 1373
Size: 5365 965 4334
Fragments arranged by size:
5365 4334 965
HindIII A'AGCT_T
Cuts at: 402 402
Size: 10664
KasI G'GCGC_C
Cuts at: 4109 4482 4702 8094 8342 4109
Size: 373 220 3392 248 6431
Fragments arranged by size:
6431 3392 373 248 220
KpnI G_GTAC'C
Cuts at: 7684 7684
Size: 10664
MluI A'CGCG_T
Cuts at: 10097 10097
Size: 10664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1613 1886 3662 3719 6596 9335 9519 1613
Size: 273 1776 57 2877 2739 184 2758
Fragments arranged by size:
2877 2758 2739 1776 273 184 57
MscI TGG'CCA
Cuts at: 2101 2164 3775 4017 4876 6719 6793 8425 2101
Size: 63 1611 242 859 1843 74 1632 4340
Fragments arranged by size:
4340 1843 1632 1611 859 242 74 63
MslI CAynn'nnrTG
Cuts at: 298 547 625 1021 2074 2857 3490 3740 4742
Size: 249 78 396 1053 783 633 250 1002
Cuts at: 4742 5594 6766 7195 7235 7330 8653 8780 298
Size: 852 1172 429 40 95 1323 127 2182
Fragments arranged by size:
2182 1323 1172 1053 1002 852 783 633
429 396 250 249 127 95 78 40
MspA1I CmG'CkG
Cuts at: 388 495 529 924 999 1456 2028 2155 3390
Size: 107 34 395 75 457 572 127 1235
Cuts at: 3390 3836 4035 4833 5001 5067 5784 6078 6219
Size: 446 199 798 168 66 717 294 141
Cuts at: 6219 6265 6821 7775 8089 8449 9462 9707 388
Size: 46 556 954 314 360 1013 245 1345
Fragments arranged by size:
1345 1235 1013 954 798 717 572 556
457 446 395 360 314 294 245 199
168 141 127 107 75 66 46 34
NaeI GCC'GGC
Cuts at: 3480 8845 3480
Size: 5365 5299
NarI GG'CG_CC
Cuts at: 4110 4483 4703 8095 8343 4110
Size: 373 220 3392 248 6431
Fragments arranged by size:
6431 3392 373 248 220
NcoI C'CATG_G
Cuts at: 107 2006 5754 5975 6882 6950 107
Size: 1899 3748 221 907 68 3821
Fragments arranged by size:
3821 3748 1899 907 221 68
NdeI CA'TA_TG
Cuts at: 6722 6722
Size: 10664
NgoMIV G'CCGG_C
Cuts at: 3478 8843 3478
Size: 5365 5299
NheI G'CTAG_C
Cuts at: 6709 6709
Size: 10664
NotI GC'GGCC_GC
Cuts at: 2401 2401
Size: 10664
NsiI A_TGCA'T
Cuts at: 3311 3311
Size: 10664
NspI r_CATG'y
Cuts at: 127 630 1092 1407 2149 2451 3735 3798 4052
Size: 503 462 315 742 302 1284 63 254
Cuts at: 4052 4402 5419 6375 7455 7717 8217 9124 127
Size: 350 1017 956 1080 262 500 907 1667
Fragments arranged by size:
1667 1284 1080 1017 956 907 742 503
500 462 350 315 302 262 254 63
PasI CC'CwG_GG
Cuts at: 337 1692 4287 5838 6336 6597 337
Size: 1355 2595 1551 498 261 4404
Fragments arranged by size:
4404 2595 1551 1355 498 261
PciI A'CATG_T
Cuts at: 626 4048 9120 626
Size: 3422 5072 2170
Fragments arranged by size:
5072 3422 2170
PflMI CCAn_nnn'nTGG
Cuts at: 2043 2262 2757 4013 4206 4392 6630 7122 2043
Size: 219 495 1256 193 186 2238 492 5585
Fragments arranged by size:
5585 2238 1256 495 492 219 193 186
PfoI T'CCnGG_A
Cuts at: 417 914 2999 3368 4025 5336 5829 6910 7253
Size: 497 2085 369 657 1311 493 1081 343
Cuts at: 7253 7352 417
Size: 99 3729
Fragments arranged by size:
3729 2085 1311 1081 657 497 493 369
343 99
PmeI GTTT'AAAC
Cuts at: 7663 7663
Size: 10664
PmlI CAC'GTG
Cuts at: 549 549
Size: 10664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1306 1338 4243 4275 4917 4949 9803 9835 1306
Size: 32 2905 32 642 32 4854 32 2135
Fragments arranged by size:
4854 2905 2135 642 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 132 509 2116 2945 3414 3990 6240 7444 132
Size: 377 1607 829 469 576 2250 1204 3352
Fragments arranged by size:
3352 2250 1607 1204 829 576 469 377
PshAI GACnn'nnGTC
Cuts at: 2467 2467
Size: 10664
PsiI TTA'TAA
Cuts at: 2773 2773
Size: 10664
PspOMI G'GGCC_C
Cuts at: 574 1064 1419 2979 3124 3996 4586 5620 5980
Size: 490 355 1560 145 872 590 1034 360
Cuts at: 5980 574
Size: 5258
Fragments arranged by size:
5258 1560 1034 872 590 490 360 355
145
PstI C_TGCA'G
Cuts at: 164 680 1754 2201 2664 2706 2991 4578 4715
Size: 516 1074 447 463 42 285 1587 137
Cuts at: 4715 5012 5039 5366 5396 6087 6227 7247 7379
Size: 297 27 327 30 691 140 1020 132
Cuts at: 7379 7711 8396 164
Size: 332 685 2432
Fragments arranged by size:
2432 1587 1074 1020 691 685 516 463
447 332 327 297 285 140 137 132
42 30 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10664
PvuII CAG'CTG
Cuts at: 388 495 924 1456 2028 3390 3836 4035 5001
Size: 107 429 532 572 1362 446 199 966
Cuts at: 5001 5784 6078 6219 6265 6821 8089 8449 388
Size: 783 294 141 46 556 1268 360 2603
Fragments arranged by size:
2603 1362 1268 966 783 572 556 532
446 429 360 294 199 141 107 46
RsrII CG'GwC_CG
Cuts at: 4914 8859 4914
Size: 3945 6719
SacI G_AGCT'C
Cuts at: 3854 7678 3854
Size: 3824 6840
SalI G'TCGA_C
Cuts at: 1371 6736 7701 1371
Size: 5365 965 4334
Fragments arranged by size:
5365 4334 965
SanDI GG'GwC_CC
Cuts at: 509 2116 2945 509
Size: 1607 829 8228
Fragments arranged by size:
8228 1607 829
SapI GCTCTTCn'nnn_
Cuts at: 2703 8687 8897 2703
Size: 5984 210 4470
Fragments arranged by size:
5984 4470 210
SbfI CC_TGCA'GG
Cuts at: 6087 7711 6087
Size: 1624 9040
ScaI AGT'ACT
Cuts at: 4309 10181 4309
Size: 5872 4792
SexAI A'CCwGG_T
Cuts at: 2327 3416 6580 2327
Size: 1089 3164 6411
Fragments arranged by size:
6411 3164 1089
SfcI C'TryA_G
Cuts at: 33 160 676 1750 2197 2660 2702 2987 3450
Size: 127 516 1074 447 463 42 285 463
Cuts at: 3450 4574 4711 5008 5035 5310 5362 5392 6073
Size: 1124 137 297 27 275 52 30 681
Cuts at: 6073 6083 6223 6231 6681 7170 7243 7375 7707
Size: 10 140 8 450 489 73 132 332
Cuts at: 7707 8392 9385 9576 33
Size: 685 993 191 1121
Fragments arranged by size:
1124 1121 1074 993 685 681 516 489
463 463 450 447 332 297 285 275
191 140 137 132 127 73 52 42
30 27 10 8
SfoI GGC'GCC
Cuts at: 4111 4484 4704 8096 8344 4111
Size: 373 220 3392 248 6431
Fragments arranged by size:
6431 3392 373 248 220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10664
SgrAI Cr'CCGG_yG
Cuts at: 4106 4106
Size: 10664
SmaI CCC'GGG
Cuts at: 2630 3973 7686 2630
Size: 1343 3713 5608
Fragments arranged by size:
5608 3713 1343
SmlI C'TyrA_G
Cuts at: 410 3061 3786 4341 4367 5721 6904 7819 8734
Size: 2651 725 555 26 1354 1183 915 915
Cuts at: 8734 9226 9488 410
Size: 492 262 1586
Fragments arranged by size:
2651 1586 1354 1183 915 915 725 555
492 262 26
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10664
SphI G_CATG'C
Cuts at: 127 2451 6375 7717 8217 127
Size: 2324 3924 1342 500 2574
Fragments arranged by size:
3924 2574 2324 1342 500
SrfI GCCC'GGGC
Cuts at: 2630 2630
Size: 10664
StuI AGG'CCT
Cuts at: 195 425 5806 7429 7474 7651 195
Size: 230 5381 1623 45 177 3208
Fragments arranged by size:
5381 3208 1623 230 177 45
StyI C'CwwG_G
Cuts at: 107 881 1707 2006 5754 5975 6882 6950 7798
Size: 774 826 299 3748 221 907 68 848
Cuts at: 7798 8152 107
Size: 354 2619
Fragments arranged by size:
3748 2619 907 848 826 774 354 299
221 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 1380 1684 2011 8247 8380 8839 9048 256
Size: 1124 304 327 6236 133 459 209 1872
Fragments arranged by size:
6236 1872 1124 459 327 304 209 133
TatI w'GTAC_w
Cuts at: 4307 5875 7160 7187 10179 4307
Size: 1568 1285 27 2992 4792
Fragments arranged by size:
4792 2992 1568 1285 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 1482 1514 2837 2869 3032 3064 3595
Size: 32 224 32 1323 32 163 32 531
Cuts at: 3595 3627 4021 4053 5629 5661 10181 10213 1226
Size: 32 394 32 1576 32 4520 32 1677
Fragments arranged by size:
4520 1677 1576 1323 531 394 224 163
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1935 8461 1935
Size: 6526 4138
XbaI T'CTAG_A
Cuts at: 7695 7695
Size: 10664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2938 5196 5851 8159 2938
Size: 2258 655 2308 5443
Fragments arranged by size:
5443 2308 2258 655
XhoI C'TCGA_G
Cuts at: 410 4367 410
Size: 3957 6707
XmaI C'CCGG_G
Cuts at: 2628 3971 7684 2628
Size: 1343 3713 5608
Fragments arranged by size:
5608 3713 1343
XmnI GAAnn'nnTTC
Cuts at: 182 3913 182
Size: 3731 6933
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI BssHII BstEII BstZ17I ClaI FseI
FspAI HpaI MfeI NruI PacI PspXI PsrI SacII
SfiI SnaBI SspI SwaI ZraI