Restriction map for
pF1KA0709
(Circular) (Six-base) MAPSORT of: pF1KA0709.seq Check: 513 from: 1 to: 7550
pF1KA0709 7550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 158 4067 158
Size: 3909 3641
AccI GT'mk_AC
Cuts at: 632 4588 632
Size: 3956 3594
Acc65I G'GTAC_C
Cuts at: 373 4566 373
Size: 4193 3357
AclI AA'CG_TT
Cuts at: 7390 7390
Size: 7550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 2790 3511 5375 5807 6160 6822 231
Size: 2559 721 1864 432 353 662 959
Fragments arranged by size:
2559 1864 959 721 662 432 353
AfeI AGC'GCT
Cuts at: 334 778 3470 3988 4328 334
Size: 444 2692 518 340 3556
Fragments arranged by size:
3556 2692 518 444 340
AflIII A'CryG_T
Cuts at: 4902 6702 6983 4902
Size: 1800 281 5469
Fragments arranged by size:
5469 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 495 495
Size: 7550
AleI CACnn'nnGTG
Cuts at: 2059 4150 2059
Size: 2091 5459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 932 1186 932
Size: 254 7296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2805 2837 2303
Size: 32 470 32 7016
Fragments arranged by size:
7016 470 32 32
AlwNI CAG_nnn'CTG
Cuts at: 387 1592 2049 2226 2241 3900 4855 6293 387
Size: 1205 457 177 15 1659 955 1438 1644
Fragments arranged by size:
1659 1644 1438 1205 955 457 177 15
ApaI G_GGCC'C
Cuts at: 116 516 1567 2028 2235 2754 3854 4175 4189
Size: 400 1051 461 207 519 1100 321 14
Cuts at: 4189 116
Size: 3477
Fragments arranged by size:
3477 1100 1051 519 461 400 321 207
14
ApaLI G'TGCA_C
Cuts at: 666 1752 2695 6388 666
Size: 1086 943 3693 1828
Fragments arranged by size:
3693 1828 1086 943
ApoI r'AATT_y
Cuts at: 1387 1461 4554 6903 7159 1387
Size: 74 3093 2349 256 1778
Fragments arranged by size:
3093 2349 1778 256 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 7550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7550
AvaI C'yCGr_G
Cuts at: 172 1134 1939 2314 2747 3483 3635 4176 4570
Size: 962 805 375 433 736 152 541 394
Cuts at: 4570 172
Size: 3152
Fragments arranged by size:
3152 962 805 736 541 433 394 375
152
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 357 390 1935 1968 2537 2570 357
Size: 33 1545 33 569 33 5337
Fragments arranged by size:
5337 1545 569 33 33 33
BamHI G'GATC_C
Cuts at: 2361 3035 4575 2361
Size: 674 1540 5336
Fragments arranged by size:
5336 1540 674
BanI G'GyrC_C
Cuts at: 176 199 373 406 541 1505 1745 2847 2930
Size: 23 174 33 135 964 240 1102 83
Cuts at: 2930 3282 3511 4087 4244 4421 4453 4566 4980
Size: 352 229 576 157 177 32 113 414
Cuts at: 4980 5228 5263 176
Size: 248 35 2463
Fragments arranged by size:
2463 1102 964 576 414 352 248 240
229 177 174 157 135 113 83 35
33 32 23
BanII G_rGCy'C
Cuts at: 116 516 1214 1520 1567 2028 2235 2564 2754
Size: 400 698 306 47 461 207 329 190
Cuts at: 2754 3296 3457 3497 3685 3854 4175 4189 4564
Size: 542 161 40 188 169 321 14 375
Cuts at: 4564 5594 116
Size: 1030 2072
Fragments arranged by size:
2072 1030 698 542 461 400 375 329
321 306 207 190 188 169 161 47
40 14
BbeI G_GCGC'C
Cuts at: 203 1749 4091 4248 4425 4984 5232 203
Size: 1546 2342 157 177 559 248 2521
Fragments arranged by size:
2521 2342 1546 559 248 177 157
BbsI GAAGACnn'nnnn_
Cuts at: 228 1724 228
Size: 1496 6054
BbvCI CC'TCA_GC
Cuts at: 3363 3363
Size: 7550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3971 4005 3971
Size: 34 7516
BciVI GTATCCnnnnn_n'
Cuts at: 5448 6504 7550 5448
Size: 1056 1046 5448
Fragments arranged by size:
5448 1056 1046
BclI T'GATC_A
Cuts at: 5070 5070
Size: 7550
BglI GCCn_nnn'nGGC
Cuts at: 120 197 1263 1571 1955 2105 2212 2579 3280
Size: 77 1066 308 384 150 107 367 701
Cuts at: 3280 3434 3586 120
Size: 154 152 4084
Fragments arranged by size:
4084 1066 701 384 367 308 154 152
150 107 77
BglII A'GATC_T
Cuts at: 2294 5065 7102 2294
Size: 2771 2037 2742
Fragments arranged by size:
2771 2742 2037
BlpI GC'TnA_GC
Cuts at: 928 1636 2242 4198 4301 4662 928
Size: 708 606 1956 103 361 3816
Fragments arranged by size:
3816 1956 708 606 361 103
Bme1580I G_kGCm'C
Cuts at: 116 394 516 670 1567 1756 2028 2056 2235
Size: 278 122 154 897 189 272 28 179
Cuts at: 2235 2699 2754 2850 3285 3439 3490 3599 3854
Size: 464 55 96 435 154 51 109 255
Cuts at: 3854 4175 4189 4458 5175 5268 6392 116
Size: 321 14 269 717 93 1124 1274
Fragments arranged by size:
1274 1124 897 717 464 435 321 278
272 269 255 189 179 154 154 122
109 96 93 55 51 28 14
BmgBI CAC'GTC
Cuts at: 1685 2553 1685
Size: 868 6682
BmrI ACTGGGnnnn_n'
Cuts at: 493 986 1080 1091 1839 2925 3288 3311 4176
Size: 493 94 11 748 1086 363 23 865
Cuts at: 4176 4762 4843 5178 7322 493
Size: 586 81 335 2144 721
Fragments arranged by size:
2144 1086 865 748 721 586 493 363
335 94 81 23 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2652 2706 3699 5917 2652
Size: 54 993 2218 4285
Fragments arranged by size:
4285 2218 993 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1205 1561 2042 2409 4461 292
Size: 913 356 481 367 2052 3381
Fragments arranged by size:
3381 2052 913 481 367 356
Bpu10I CC'TnA_GC
Cuts at: 2722 3363 3451 2722
Size: 641 88 6821
Fragments arranged by size:
6821 641 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1042 2561 4726 5605 6319 6617 568
Size: 474 1519 2165 879 714 298 1501
Fragments arranged by size:
2165 1519 1501 879 714 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 351 808 3403 3708 7254 351
Size: 457 2595 305 3546 647
Fragments arranged by size:
3546 2595 647 457 305
BsaAI yAC'GTr
Cuts at: 2642 2669 3594 3726 3774 4903 2642
Size: 27 925 132 48 1129 5289
Fragments arranged by size:
5289 1129 925 132 48 27
BsaBI GATnn'nnATC
Cuts at: 6 5064 7101 6
Size: 5058 2037 455
Fragments arranged by size:
5058 2037 455
BsaHI Gr'CG_yC
Cuts at: 200 212 1746 2065 4088 4245 4422 4981 5229
Size: 12 1534 319 2023 157 177 559 248
Cuts at: 5229 5931 7427 200
Size: 702 1496 323
Fragments arranged by size:
2023 1534 1496 702 559 323 319 248
177 157 12
BsaWI w'CCGG_w
Cuts at: 2144 2426 4415 4739 5260 5938 6349 6496 6770
Size: 282 1989 324 521 678 411 147 274
Cuts at: 6770 2144
Size: 2924
Fragments arranged by size:
2924 1989 678 521 411 324 282 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1411 1441 1825 1855 2464 2494 2599 2629 2824
Size: 30 384 30 609 30 105 30 195
Cuts at: 2824 2854 4027 4057 1411
Size: 30 1173 30 4904
Fragments arranged by size:
4904 1173 609 384 195 105 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 613 961 1117 1435 1669 2275 2278 3421
Size: 453 348 156 318 234 606 3 1143
Cuts at: 3421 3691 3724 3848 160
Size: 270 33 124 3862
Fragments arranged by size:
3862 1143 606 453 348 318 270 234
156 124 33 3
BseYI C'CCAG_C
Cuts at: 329 779 1710 2095 2123 2195 2226 2234 3134
Size: 450 931 385 28 72 31 8 900
Cuts at: 3134 3383 3462 3963 4095 4189 4341 4975 5733
Size: 249 79 501 132 94 152 634 758
Cuts at: 5733 6398 6806 329
Size: 665 408 1073
Fragments arranged by size:
1073 931 900 758 665 634 501 450
408 385 249 152 132 94 79 72
31 28 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 693 2649 2764 3337 3384 166
Size: 527 1956 115 573 47 4332
Fragments arranged by size:
4332 1956 573 527 115 47
BsiEI CG_ry'CG
Cuts at: 104 120 124 192 1208 2427 3393 5092 5138
Size: 16 4 68 1016 1219 966 1699 46
Cuts at: 5138 6368 104
Size: 1230 1286
Fragments arranged by size:
1699 1286 1230 1219 1016 966 68 46
16 4
BsiHKAI G_wGCw'C
Cuts at: 615 670 1756 2270 2633 2699 3497 3935 4058
Size: 55 1086 514 363 66 798 438 123
Cuts at: 4058 4564 5342 5532 6392 615
Size: 506 778 190 860 1773
Fragments arranged by size:
1773 1086 860 798 778 514 506 438
363 190 123 66 55
BsmI GAATG_Cn'
Cuts at: 3169 3169
Size: 7550
Bsp1286I G_dGCh'C
Cuts at: 116 394 516 615 670 1214 1520 1567 1756
Size: 278 122 99 55 544 306 47 189
Cuts at: 1756 2028 2056 2235 2270 2564 2633 2699 2754
Size: 272 28 179 35 294 69 66 55
Cuts at: 2754 2850 3285 3296 3439 3457 3490 3497 3599
Size: 96 435 11 143 18 33 7 102
Cuts at: 3599 3685 3854 3935 4058 4175 4189 4458 4564
Size: 86 169 81 123 117 14 269 106
Cuts at: 4564 5175 5268 5342 5532 5594 6392 116
Size: 611 93 74 190 62 798 1274
Fragments arranged by size:
1274 798 611 544 435 306 294 278
272 269 190 189 179 169 143 123
122 117 106 102 99 96 93 86
81 74 69 66 62 55 55 47
35 33 28 18 14 11 7
BspHI T'CATG_A
Cuts at: 5982 5982
Size: 7550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 279 3005 4067 4600 4814 5116 5497 158
Size: 121 2726 1062 533 214 302 381 2211
Fragments arranged by size:
2726 2211 1062 533 381 302 214 121
BsrBI CCG'CTC
Cuts at: 50 1025 2175 3654 4000 5842 5896 7380 7545
Size: 975 1150 1479 346 1842 54 1484 165
Cuts at: 7545 50
Size: 55
Fragments arranged by size:
1842 1484 1479 1150 975 346 165 55
54
BsrDI GCAATG_nn'
Cuts at: 5462 5462
Size: 7550
BsrFI r'CCGG_y
Cuts at: 119 123 356 1876 2039 2432 5548 5729 119
Size: 4 233 1520 163 393 3116 181 1940
Fragments arranged by size:
3116 1940 1520 393 233 181 163 4
BsrGI T'GTAC_A
Cuts at: 1913 2891 1913
Size: 978 6572
BssSI C'ACGA_G
Cuts at: 625 946 1020 2323 4295 5821 6529 625
Size: 321 74 1303 1972 1526 708 1646
Fragments arranged by size:
1972 1646 1526 1303 708 321 74
BstAPI GCAn_nnn'nTGC
Cuts at: 4927 4927
Size: 7550
BstBI TT'CG_AA
Cuts at: 5911 5911
Size: 7550
BstEII G'GTnAC_C
Cuts at: 487 556 733 1776 3141 487
Size: 69 177 1043 1365 4896
Fragments arranged by size:
4896 1365 1043 177 69
BstXI CCAn_nnnn'nTGG
Cuts at: 1229 3376 1229
Size: 2147 5403
BstYI r'GATC_y
Cuts at: 1 921 941 2085 2294 2318 2361 2525 2534
Size: 920 20 1144 209 24 43 164 9
Cuts at: 2534 3035 4575 5042 5065 5400 5646 5952 5964
Size: 501 1540 467 23 335 246 306 12
Cuts at: 5964 6050 7102 1
Size: 86 1052 449
Fragments arranged by size:
1540 1144 1052 920 501 467 449 335
306 246 209 164 86 43 24 23
20 12 9
BtgI C'CryG_G
Cuts at: 107 252 416 654 702 1202 1662 1761 1950
Size: 145 164 238 48 500 460 99 189
Cuts at: 1950 1985 3414 3879 4271 107
Size: 35 1429 465 392 3386
Fragments arranged by size:
3386 1429 500 465 460 392 238 189
164 145 99 48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1149 2810 5500 5681 5844 1149
Size: 1661 2690 181 163 2855
Fragments arranged by size:
2855 2690 1661 181 163
BtsI GCAGTG_nn'
Cuts at: 388 576 1042 1281 2494 2509 3852 3933 4793
Size: 188 466 239 1213 15 1343 81 860
Cuts at: 4793 388
Size: 3145
Fragments arranged by size:
3145 1343 1213 860 466 239 188 81
15
DraI TTT'AAA
Cuts at: 4549 4549
Size: 7550
DraIII CAC_nnn'GTG
Cuts at: 487 995 2274 487
Size: 508 1279 5763
Fragments arranged by size:
5763 1279 508
DrdI GACnn_nn'nnGTC
Cuts at: 5256 6600 5256
Size: 1344 6206
EaeI y'GGCC_r
Cuts at: 117 121 189 400 573 1205 1970 2249 2276
Size: 4 68 211 173 632 765 279 27
Cuts at: 2276 2495 3378 5135 5309 5700 5727 7072 117
Size: 219 883 1757 174 391 27 1345 595
Fragments arranged by size:
1757 1345 883 765 632 595 391 279
219 211 174 173 68 27 27 4
EagI C'GGCC_G
Cuts at: 117 121 189 1205 5135 117
Size: 4 68 1016 3930 2532
Fragments arranged by size:
3930 2532 1016 68 4
EarI CTCTTCn'nnn_
Cuts at: 2185 5573 5783 2185
Size: 3388 210 3952
Fragments arranged by size:
3952 3388 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1393 2722 5758 6500 6646 7360 1393
Size: 1329 3036 742 146 714 1583
Fragments arranged by size:
3036 1583 1329 742 714 146
EcoICRI GAG'CTC
Cuts at: 3495 4562 3495
Size: 1067 6483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 292 1205 1561 2042 2409 2790 3511 4461
Size: 61 913 356 481 367 381 721 950
Cuts at: 4461 5375 5807 6160 6822 231
Size: 914 432 353 662 959
Fragments arranged by size:
959 950 914 913 721 662 481 432
381 367 356 353 61
EcoNI CCTnn'n_nnAGG
Cuts at: 3231 3625 3231
Size: 394 7156
EcoO109I rG'GnC_Cy
Cuts at: 112 512 603 1563 1863 2213 2231 3788 3851
Size: 400 91 960 300 350 18 1557 63
Cuts at: 3851 4171 4172 4186 4442 4689 112
Size: 320 1 14 256 247 2973
Fragments arranged by size:
2973 1557 960 400 350 320 300 256
247 91 63 18 14 1
EcoRI G'AATT_C
Cuts at: 1387 4554 1387
Size: 3167 4383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5569 5569
Size: 7550
FseI GG_CCGG'CC
Cuts at: 123 127 123
Size: 4 7546
FspI TGC'GCA
Cuts at: 3646 5331 3646
Size: 1685 5865
HaeII r_GCGC'y
Cuts at: 203 336 780 1749 1969 1985 2388 2805 3472
Size: 133 444 969 220 16 403 417 667
Cuts at: 3472 3990 4091 4248 4256 4330 4425 4984 5232
Size: 518 101 157 8 74 95 559 248
Cuts at: 5232 6462 7233 203
Size: 1230 771 520
Fragments arranged by size:
1230 969 771 667 559 520 518 444
417 403 248 220 157 133 101 95
74 16 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 1750 1782 2464 2496 5392 5422 5424
Size: 32 1237 32 682 32 2896 30 2
Cuts at: 5424 5454 481
Size: 30 2577
Fragments arranged by size:
2896 2577 1237 682 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4589 4589
Size: 7550
HindIII A'AGCT_T
Cuts at: 4288 4288
Size: 7550
KasI G'GCGC_C
Cuts at: 199 1745 4087 4244 4421 4980 5228 199
Size: 1546 2342 157 177 559 248 2521
Fragments arranged by size:
2521 2342 1546 559 248 177 157
KpnI G_GTAC'C
Cuts at: 377 4570 377
Size: 4193 3357
MluI A'CGCG_T
Cuts at: 6983 6983
Size: 7550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1660 4187 6309 6493 1022
Size: 638 2527 2122 184 2079
Fragments arranged by size:
2527 2122 2079 638 184
MscI TGG'CCA
Cuts at: 402 575 1972 2251 2278 2497 5311 402
Size: 173 1397 279 27 219 2814 2641
Fragments arranged by size:
2814 2641 1397 279 219 173 27
MslI CAynn'nnrTG
Cuts at: 2059 4150 4270 4510 4525 5539 5666 2059
Size: 2091 120 240 15 1014 127 3943
Fragments arranged by size:
3943 2091 1014 240 127 120 15
MspA1I CmG'CkG
Cuts at: 495 837 848 902 1313 1577 1635 1773 2238
Size: 342 11 54 411 264 58 138 465
Cuts at: 2238 2517 3138 3338 3383 3537 3717 3806 3887
Size: 279 621 200 45 154 180 89 81
Cuts at: 3887 4150 4211 4235 4345 4661 4975 5335 6119
Size: 263 61 24 110 316 314 360 784
Cuts at: 6119 6364 495
Size: 245 1681
Fragments arranged by size:
1681 784 621 465 411 360 342 316
314 279 264 263 245 200 180 154
138 110 89 81 61 58 54 45
24 11
NaeI GCC'GGC
Cuts at: 121 125 2041 2434 5731 121
Size: 4 1916 393 3297 1940
Fragments arranged by size:
3297 1940 1916 393 4
NarI GG'CG_CC
Cuts at: 200 1746 4088 4245 4422 4981 5229 200
Size: 1546 2342 157 177 559 248 2521
Fragments arranged by size:
2521 2342 1546 559 248 177 157
NcoI C'CATG_G
Cuts at: 107 252 1662 1761 1985 3414 3879 4271 107
Size: 145 1410 99 224 1429 465 392 3386
Fragments arranged by size:
3386 1429 1410 465 392 224 145 99
NgoMIV G'CCGG_C
Cuts at: 119 123 2039 2432 5729 119
Size: 4 1916 393 3297 1940
Fragments arranged by size:
3297 1940 1916 393 4
NspI r_CATG'y
Cuts at: 435 1337 2286 4197 4603 5103 6706 435
Size: 902 949 1911 406 500 1603 1279
Fragments arranged by size:
1911 1603 1279 949 902 500 406
PasI CC'CwG_GG
Cuts at: 205 279 426 2118 2208 3012 3894 205
Size: 74 147 1692 90 804 882 3861
Fragments arranged by size:
3861 1692 882 804 147 90 74
PciI A'CATG_T
Cuts at: 6702 6702
Size: 7550
PflMI CCAn_nnn'nTGG
Cuts at: 995 1374 2226 3249 3900 995
Size: 379 852 1023 651 4645
Fragments arranged by size:
4645 1023 852 651 379
PfoI T'CCnGG_A
Cuts at: 221 1529 2603 3926 4022 221
Size: 1308 1074 1323 96 3749
Fragments arranged by size:
3749 1323 1308 1074 96
PmeI GTTT'AAAC
Cuts at: 4549 4549
Size: 7550
PmlI CAC'GTG
Cuts at: 2642 2669 2642
Size: 27 7523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 2031 2605 2637 3094 3126 5996 6028 1999
Size: 32 574 32 457 32 2870 32 3521
Fragments arranged by size:
3521 2870 574 457 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 603 1863 603
Size: 1260 6290
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 7550
PspOMI G'GGCC_C
Cuts at: 112 512 1563 2024 2231 2750 3850 4171 4185
Size: 400 1051 461 207 519 1100 321 14
Cuts at: 4185 112
Size: 3477
Fragments arranged by size:
3477 1100 1051 519 461 400 321 207
14
PstI C_TGCA'G
Cuts at: 263 1162 1298 1457 1862 2183 2489 3824 4064
Size: 899 136 159 405 321 306 1335 240
Cuts at: 4064 4597 5282 263
Size: 533 685 2531
Fragments arranged by size:
2531 1335 899 685 533 405 321 306
240 159 136
PvuI CG_AT'CG
Cuts at: 104 3393 104
Size: 3289 4261
PvuII CAG'CTG
Cuts at: 495 837 848 902 1313 1635 1773 2238 2517
Size: 342 11 54 411 322 138 465 279
Cuts at: 2517 3138 3338 3806 3887 4150 4211 4975 5335
Size: 621 200 468 81 263 61 764 360
Cuts at: 5335 495
Size: 2710
Fragments arranged by size:
2710 764 621 468 465 411 360 342
322 279 263 200 138 81 61 54
11
RsrII CG'GwC_CG
Cuts at: 5745 5745
Size: 7550
SacI G_AGCT'C
Cuts at: 3497 4564 3497
Size: 1067 6483
SalI G'TCGA_C
Cuts at: 4587 4587
Size: 7550
SapI GCTCTTCn'nnn_
Cuts at: 2185 5573 5783 2185
Size: 3388 210 3952
Fragments arranged by size:
3952 3388 210
SbfI CC_TGCA'GG
Cuts at: 1298 1862 4597 1298
Size: 564 2735 4251
Fragments arranged by size:
4251 2735 564
ScaI AGT'ACT
Cuts at: 1843 7067 1843
Size: 5224 2326
SexAI A'CCwGG_T
Cuts at: 1355 1355
Size: 7550
SfcI C'TryA_G
Cuts at: 33 259 1158 1294 1453 1731 1858 2179 2485
Size: 226 899 136 159 278 127 321 306
Cuts at: 2485 3005 3820 4060 4461 4593 5278 6246 6437
Size: 520 815 240 401 132 685 968 191
Cuts at: 6437 33
Size: 1146
Fragments arranged by size:
1146 968 899 815 685 520 401 321
306 278 240 226 191 159 136 132
127
SfiI GGCCn_nnn'nGGCC
Cuts at: 120 1571 2212 120
Size: 1451 641 5458
Fragments arranged by size:
5458 1451 641
SfoI GGC'GCC
Cuts at: 201 1747 4089 4246 4423 4982 5230 201
Size: 1546 2342 157 177 559 248 2521
Fragments arranged by size:
2521 2342 1546 559 248 177 157
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7550
SmaI CCC'GGG
Cuts at: 1941 2749 3485 4178 4572 1941
Size: 808 736 693 394 4919
Fragments arranged by size:
4919 808 736 693 394
SmlI C'TyrA_G
Cuts at: 547 1057 2576 4705 5620 6334 6596 547
Size: 510 1519 2129 915 714 262 1501
Fragments arranged by size:
2129 1519 1501 915 714 510 262
SnaBI TAC'GTA
Cuts at: 3774 3774
Size: 7550
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7550
SphI G_CATG'C
Cuts at: 4197 4603 5103 4197
Size: 406 500 6644
Fragments arranged by size:
6644 500 406
StuI AGG'CCT
Cuts at: 892 1804 3616 892
Size: 912 1812 4826
Fragments arranged by size:
4826 1812 912
StyI C'CwwG_G
Cuts at: 107 252 1662 1761 1985 3414 3879 4271 4684
Size: 145 1410 99 224 1429 465 392 413
Cuts at: 4684 5038 107
Size: 354 2619
Fragments arranged by size:
2619 1429 1410 465 413 392 354 224
145 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 769 1513 1933 2481 3237 3625 4264 5133
Size: 294 744 420 548 756 388 639 869
Cuts at: 5133 5266 5725 5934 475
Size: 133 459 209 2091
Fragments arranged by size:
2091 869 756 744 639 548 459 420
388 294 209 133
TatI w'GTAC_w
Cuts at: 1841 1913 2615 2891 7065 1841
Size: 72 702 276 4174 2326
Fragments arranged by size:
4174 2326 702 276 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1411 1443 1681 1713 2869 2901 2914 2946 3229
Size: 32 238 32 1156 32 13 32 283
Cuts at: 3229 3261 7067 7099 1411
Size: 32 3806 32 1862
Fragments arranged by size:
3806 1862 1156 283 238 32 32 32
32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1357 1549 5347 1357
Size: 192 3798 3560
Fragments arranged by size:
3798 3560 192
XbaI T'CTAG_A
Cuts at: 4581 4581
Size: 7550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 423 568 1242 1703 3342 4000 4269 5045
Size: 39 145 674 461 1639 658 269 776
Cuts at: 5045 384
Size: 2889
Fragments arranged by size:
2889 1639 776 674 658 461 269 145
39
XmaI C'CCGG_G
Cuts at: 1939 2747 3483 4176 4570 1939
Size: 808 736 693 394 4919
Fragments arranged by size:
4919 808 736 693 394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AscI AvrII BfrBI BmtI BsiWI
BsmBI BspEI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PspXI PsrI SacII SanDI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI