Restriction map for
pF1KA0714
(Circular) (Six-base) MAPSORT of: pF1KA0714.seq Check: 1166 from: 1 to: 8411
pF1KA0714 8411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 3546 5222 5449 3546
Size: 1676 227 6508
Fragments arranged by size:
6508 1676 227
Acc65I G'GTAC_C
Cuts at: 5427 5427
Size: 8411
AclI AA'CG_TT
Cuts at: 5229 8251 5229
Size: 3022 5389
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 668 1298 1657 1763 1826 2378 2657 2836
Size: 378 630 359 106 63 552 279 179
Cuts at: 2836 3092 3488 4266 6236 6668 7415 7683 290
Size: 256 396 778 1970 432 747 268 1018
Fragments arranged by size:
1970 1018 778 747 630 552 432 396
378 359 279 268 256 179 106 63
AflII C'TTAA_G
Cuts at: 2254 2254
Size: 8411
AflIII A'CryG_T
Cuts at: 2448 3300 5763 6867 7844 2448
Size: 852 2463 1104 977 3015
Fragments arranged by size:
3015 2463 1104 977 852
AgeI A'CCGG_T
Cuts at: 6799 6799
Size: 8411
AleI CACnn'nnGTG
Cuts at: 3210 3210
Size: 8411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3941 3941
Size: 8411
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5108 5140 5108
Size: 32 8379
AlwNI CAG_nnn'CTG
Cuts at: 205 400 1627 2103 2545 5716 7283 205
Size: 195 1227 476 442 3171 1567 1333
Fragments arranged by size:
3171 1567 1333 1227 476 442 195
ApaLI G'TGCA_C
Cuts at: 7181 7181
Size: 8411
ApoI r'AATT_y
Cuts at: 364 431 443 766 856 1257 2244 2745 3529
Size: 67 12 323 90 401 987 501 784
Cuts at: 3529 4033 5326 5415 7764 8020 364
Size: 504 1293 89 2349 256 755
Fragments arranged by size:
2349 1293 987 784 755 504 501 401
323 256 90 89 67 12
AseI AT'TA_AT
Cuts at: 20 1913 4555 20
Size: 1893 2642 3876
Fragments arranged by size:
3876 2642 1893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8411
AvaI C'yCGr_G
Cuts at: 4991 5003 5431 4991
Size: 12 428 7971
Fragments arranged by size:
7971 428 12
AvrII C'CTAG_G
Cuts at: 240 240
Size: 8411
BamHI G'GATC_C
Cuts at: 5436 5436
Size: 8411
BanI G'GyrC_C
Cuts at: 5427 5841 6089 6124 5427
Size: 414 248 35 7714
Fragments arranged by size:
7714 414 248 35
BanII G_rGCy'C
Cuts at: 1003 1690 4745 5425 6455 1003
Size: 687 3055 680 1030 2959
Fragments arranged by size:
3055 2959 1030 687 680
BbeI G_GCGC'C
Cuts at: 5845 6093 5845
Size: 248 8163
BbsI GAAGACnn'nnnn_
Cuts at: 1886 4520 1886
Size: 2634 5777
BciVI GTATCCnnnnn_n'
Cuts at: 3981 4528 6309 7070 8411 3981
Size: 547 1781 761 1341 3981
Fragments arranged by size:
3981 1781 1341 761 547
BclI T'GATC_A
Cuts at: 453 1413 4353 5931 453
Size: 960 2940 1578 2933
Fragments arranged by size:
2940 2933 1578 960
BfrBI ATG'CAT
Cuts at: 613 613
Size: 8411
BglII A'GATC_T
Cuts at: 1595 3576 5926 7963 1595
Size: 1981 2350 2037 2043
Fragments arranged by size:
2350 2043 2037 1981
BlpI GC'TnA_GC
Cuts at: 924 2604 5199 5523 924
Size: 1680 2595 324 3812
Fragments arranged by size:
3812 2595 1680 324
Bme1580I G_kGCm'C
Cuts at: 6036 6129 7185 6036
Size: 93 1056 7262
Fragments arranged by size:
7262 1056 93
BmgBI CAC'GTC
Cuts at: 1268 1268
Size: 8411
BmrI ACTGGGnnnn_n'
Cuts at: 3769 5089 5623 5704 6039 8183 3769
Size: 1320 534 81 335 2144 3997
Fragments arranged by size:
3997 2144 1320 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 3002 6778 960
Size: 2042 3776 2593
Fragments arranged by size:
3776 2593 2042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 4228 4729 868
Size: 3360 501 4550
Fragments arranged by size:
4550 3360 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 5587 6466 6958 7256 3196
Size: 2391 879 492 298 4351
Fragments arranged by size:
4351 2391 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3377 8115 3377
Size: 4738 3673
BsaAI yAC'GTr
Cuts at: 3287 3301 5764 3287
Size: 14 2463 5934
Fragments arranged by size:
5934 2463 14
BsaBI GATnn'nnATC
Cuts at: 6 2918 5925 7962 6
Size: 2912 3007 2037 455
Fragments arranged by size:
3007 2912 2037 455
BsaHI Gr'CG_yC
Cuts at: 5842 6090 6792 8288 5842
Size: 248 702 1496 5965
Fragments arranged by size:
5965 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2174 3782 5600 6121 6799 7073 7220 7631 2174
Size: 1608 1818 521 678 274 147 411 2954
Fragments arranged by size:
2954 1818 1608 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 5108 5138 5108
Size: 30 8381
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 1847 4267 4753 865
Size: 982 2420 486 4523
Fragments arranged by size:
4523 2420 982 486
BseYI C'CCAG_C
Cuts at: 1047 1279 1802 3091 5836 6594 7171 7667 1047
Size: 232 523 1289 2745 758 577 496 1791
Fragments arranged by size:
2745 1791 1289 758 577 523 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 2104
Size: 8411
BsiEI CG_ry'CG
Cuts at: 104 5953 5999 7207 104
Size: 5849 46 1208 1308
Fragments arranged by size:
5849 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1690 2172 2673 3540 3856 4745 5425 6203 6393
Size: 482 501 867 316 889 680 778 190
Cuts at: 6393 7185 1690
Size: 792 2916
Fragments arranged by size:
2916 889 867 792 778 680 501 482
316 190
BsmI GAATG_Cn'
Cuts at: 962 1354 4131 4436 962
Size: 392 2777 305 4937
Fragments arranged by size:
4937 2777 392 305
BsmBI CGTCTCn'nnnn_
Cuts at: 1274 2468 5389 1274
Size: 1194 2921 4296
Fragments arranged by size:
4296 2921 1194
Bsp1286I G_dGCh'C
Cuts at: 1003 1690 2172 2673 3540 3856 4745 5425 6036
Size: 687 482 501 867 316 889 680 611
Cuts at: 6036 6129 6203 6393 6455 7185 1003
Size: 93 74 190 62 730 2229
Fragments arranged by size:
2229 889 867 730 687 680 611 501
482 316 190 93 74 62
BspEI T'CCGG_A
Cuts at: 7631 7631
Size: 8411
BspHI T'CATG_A
Cuts at: 456 2508 7587 456
Size: 2052 5079 1280
Fragments arranged by size:
5079 2052 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3558 5461 5675 5977 6358 3558
Size: 1903 214 302 381 5611
Fragments arranged by size:
5611 1903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6703 6757 8241 8406 50
Size: 6653 54 1484 165 55
Fragments arranged by size:
6653 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4136 6323 4136
Size: 2187 6224
BsrFI r'CCGG_y
Cuts at: 6409 6590 6799 6409
Size: 181 209 8021
Fragments arranged by size:
8021 209 181
BsrGI T'GTAC_A
Cuts at: 379 2064 2162 4947 5345 379
Size: 1685 98 2785 398 3445
Fragments arranged by size:
3445 2785 1685 398 98
BssSI C'ACGA_G
Cuts at: 6682 7040 6682
Size: 358 8053
BstAPI GCAn_nnn'nTGC
Cuts at: 610 5788 610
Size: 5178 3233
BstBI TT'CG_AA
Cuts at: 6772 6772
Size: 8411
BstXI CCAn_nnnn'nTGG
Cuts at: 1055 3984 5179 1055
Size: 2929 1195 4287
Fragments arranged by size:
4287 2929 1195
BstYI r'GATC_y
Cuts at: 1 1595 3576 5436 5903 5926 6261 6507 7519
Size: 1594 1981 1860 467 23 335 246 1012
Cuts at: 7519 7605 7617 7963 1
Size: 86 12 346 449
Fragments arranged by size:
1981 1860 1594 1012 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 3547 3547
Size: 8411
Bsu36I CC'TnA_GG
Cuts at: 145 145
Size: 8411
BtgI C'CryG_G
Cuts at: 107 4838 107
Size: 4731 3680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6361 6542 6705 6361
Size: 181 163 8067
Fragments arranged by size:
8067 181 163
BtsI GCAGTG_nn'
Cuts at: 2559 3004 3205 3217 4869 4923 5137 5654 2559
Size: 445 201 12 1652 54 214 517 5316
Fragments arranged by size:
5316 1652 517 445 214 201 54 12
DraI TTT'AAA
Cuts at: 1998 5410 1998
Size: 3412 4999
DraIII CAC_nnn'GTG
Cuts at: 727 3368 4510 727
Size: 2641 1142 4628
Fragments arranged by size:
4628 2641 1142
DrdI GACnn_nn'nnGTC
Cuts at: 466 1039 6117 6975 466
Size: 573 5078 858 1902
Fragments arranged by size:
5078 1902 858 573
EaeI y'GGCC_r
Cuts at: 162 424 5996 6170 6561 6588 7933 162
Size: 262 5572 174 391 27 1345 640
Fragments arranged by size:
5572 1345 640 391 262 174 27
EagI C'GGCC_G
Cuts at: 5996 5996
Size: 8411
EarI CTCTTCn'nnn_
Cuts at: 1317 1382 1678 2255 2429 2993 4285 4385 4433
Size: 65 296 577 174 564 1292 100 48
Cuts at: 4433 6434 6644 1317
Size: 2001 210 3084
Fragments arranged by size:
3084 2001 1292 577 564 296 210 174
100 65 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5151 6619 6929 7075 8221 5151
Size: 1468 310 146 1146 5341
Fragments arranged by size:
5341 1468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1688 4743 5423 1688
Size: 3055 680 4676
Fragments arranged by size:
4676 3055 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 668 868 1298 1657 1763 1826 2378 2657
Size: 378 200 430 359 106 63 552 279
Cuts at: 2657 2836 3092 3488 4228 4266 4729 6236 6668
Size: 179 256 396 740 38 463 1507 432
Cuts at: 6668 7415 7683 290
Size: 747 268 1018
Fragments arranged by size:
1507 1018 747 740 552 463 432 430
396 378 359 279 268 256 200 179
106 63 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1663 1663
Size: 8411
EcoO109I rG'GnC_Cy
Cuts at: 4704 4716 5550 4704
Size: 12 834 7565
Fragments arranged by size:
7565 834 12
EcoRI G'AATT_C
Cuts at: 2244 5415 2244
Size: 3171 5240
EcoRV GAT'ATC
Cuts at: 2325 2847 2325
Size: 522 7889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 2250 6430 430
Size: 1820 4180 2411
Fragments arranged by size:
4180 2411 1820
FspI TGC'GCA
Cuts at: 6192 6192
Size: 8411
HaeII r_GCGC'y
Cuts at: 5845 6093 7115 8094 5845
Size: 248 1022 979 6162
Fragments arranged by size:
6162 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 1621 1653 2365 2397 4526 4558 6253
Size: 32 616 32 712 32 2129 32 1695
Cuts at: 6253 6283 6285 6315 973
Size: 30 2 30 3069
Fragments arranged by size:
3069 2129 1695 712 616 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1289 2346 5450 1289
Size: 1057 3104 4250
Fragments arranged by size:
4250 3104 1057
HindIII A'AGCT_T
Cuts at: 488 2059 2430 488
Size: 1571 371 6469
Fragments arranged by size:
6469 1571 371
KasI G'GCGC_C
Cuts at: 5841 6089 5841
Size: 248 8163
KpnI G_GTAC'C
Cuts at: 5431 5431
Size: 8411
MfeI C'AATT_G
Cuts at: 1037 1037
Size: 8411
MluI A'CGCG_T
Cuts at: 7844 7844
Size: 8411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 3851 4461 4702 7082 7266 1223
Size: 2628 610 241 2380 184 2368
Fragments arranged by size:
2628 2380 2368 610 241 184
MscI TGG'CCA
Cuts at: 426 6172 426
Size: 5746 2665
MslI CAynn'nnrTG
Cuts at: 1109 3210 5177 6400 6527 1109
Size: 2101 1967 1223 127 2993
Fragments arranged by size:
2993 2101 1967 1223 127
MspA1I CmG'CkG
Cuts at: 592 3333 3938 4749 5522 5836 6196 7209 7454
Size: 2741 605 811 773 314 360 1013 245
Cuts at: 7454 592
Size: 1549
Fragments arranged by size:
2741 1549 1013 811 773 605 360 314
245
NaeI GCC'GGC
Cuts at: 6592 6592
Size: 8411
NarI GG'CG_CC
Cuts at: 5842 6090 5842
Size: 248 8163
NcoI C'CATG_G
Cuts at: 107 4838 107
Size: 4731 3680
NdeI CA'TA_TG
Cuts at: 4316 4814 4316
Size: 498 7913
NgoMIV G'CCGG_C
Cuts at: 6590 6590
Size: 8411
NsiI A_TGCA'T
Cuts at: 615 615
Size: 8411
NspI r_CATG'y
Cuts at: 2276 2296 2452 5318 5464 5964 6871 2276
Size: 20 156 2866 146 500 907 3816
Fragments arranged by size:
3816 2866 907 500 156 146 20
PciI A'CATG_T
Cuts at: 2448 6867 2448
Size: 4419 3992
PfoI T'CCnGG_A
Cuts at: 3691 3691
Size: 8411
PmeI GTTT'AAAC
Cuts at: 5410 5410
Size: 8411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1388 1420 1811 1843 5108 5140 7550 7582 1388
Size: 32 391 32 3265 32 2410 32 2217
Fragments arranged by size:
3265 2410 2217 391 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4704 4716 4704
Size: 12 8399
PsiI TTA'TAA
Cuts at: 703 1363 3437 5053 703
Size: 660 2074 1616 4061
Fragments arranged by size:
4061 2074 1616 660
PspXI vC'TCGA_Gb
Cuts at: 4991 4991
Size: 8411
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 363 395 5039 5071 363
Size: 32 4644 32 3703
Fragments arranged by size:
4644 3703 32 32
PstI C_TGCA'G
Cuts at: 174 692 3212 5153 5458 6143 174
Size: 518 2520 1941 305 685 2442
Fragments arranged by size:
2520 2442 1941 685 518 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8411
PvuII CAG'CTG
Cuts at: 592 3333 3938 4749 5836 6196 592
Size: 2741 605 811 1087 360 2807
Fragments arranged by size:
2807 2741 1087 811 605 360
RsrII CG'GwC_CG
Cuts at: 6606 6606
Size: 8411
SacI G_AGCT'C
Cuts at: 1690 4745 5425 1690
Size: 3055 680 4676
Fragments arranged by size:
4676 3055 680
SalI G'TCGA_C
Cuts at: 5448 5448
Size: 8411
SapI GCTCTTCn'nnn_
Cuts at: 1317 1678 4385 6434 6644 1317
Size: 361 2707 2049 210 3084
Fragments arranged by size:
3084 2707 2049 361 210
SbfI CC_TGCA'GG
Cuts at: 5458 5458
Size: 8411
ScaI AGT'ACT
Cuts at: 7928 7928
Size: 8411
SfcI C'TryA_G
Cuts at: 33 170 688 3208 5149 5307 5454 6139 7132
Size: 137 518 2520 1941 158 147 685 993
Cuts at: 7132 7323 33
Size: 191 1121
Fragments arranged by size:
2520 1941 1121 993 685 518 191 158
147 137
SfoI GGC'GCC
Cuts at: 5843 6091 5843
Size: 248 8163
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8411
SmaI CCC'GGG
Cuts at: 5433 5433
Size: 8411
SmlI C'TyrA_G
Cuts at: 2254 3175 4991 5003 5566 6481 6973 7235 2254
Size: 921 1816 12 563 915 492 262 3430
Fragments arranged by size:
3430 1816 921 915 563 492 262 12
SnaBI TAC'GTA
Cuts at: 3287 3287
Size: 8411
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8411
SphI G_CATG'C
Cuts at: 5464 5964 5464
Size: 500 7911
SspI AAT'ATT
Cuts at: 3282 3674 4884 3282
Size: 392 1210 6809
Fragments arranged by size:
6809 1210 392
StuI AGG'CCT
Cuts at: 150 1847 4293 150
Size: 1697 2446 4268
Fragments arranged by size:
4268 2446 1697
StyI C'CwwG_G
Cuts at: 107 240 2225 2308 2328 4044 4838 5545 5899
Size: 133 1985 83 20 1716 794 707 354
Cuts at: 5899 107
Size: 2619
Fragments arranged by size:
2619 1985 1716 794 707 354 133 83
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1636 3751 5185 5994 6127 6586 6795 1636
Size: 2115 1434 809 133 459 209 3252
Fragments arranged by size:
3252 2115 1434 809 459 209 133
TatI w'GTAC_w
Cuts at: 379 1065 2064 2162 3103 3303 4947 4957 5345
Size: 686 999 98 941 200 1644 10 388
Cuts at: 5345 7926 379
Size: 2581 864
Fragments arranged by size:
2581 1644 999 941 864 686 388 200
98 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4848 4880 7928 7960 4848
Size: 32 3048 32 5299
Fragments arranged by size:
5299 3048 32 32
Tth111I GACn'n_nGTC
Cuts at: 1885 6208 1885
Size: 4323 4088
XbaI T'CTAG_A
Cuts at: 2901 5442 2901
Size: 2541 5870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5906 5906
Size: 8411
XhoI C'TCGA_G
Cuts at: 4991 5003 4991
Size: 12 8399
XmaI C'CCGG_G
Cuts at: 5431 5431
Size: 8411
XmnI GAAnn'nnTTC
Cuts at: 3266 3408 5326 3266
Size: 142 1918 6351
Fragments arranged by size:
6351 1918 142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI BaeI BbvCI
BcgI BglI BmtI Bpu10I BsiWI BssHII BstEII ClaI
CspCI FseI FspAI HpaI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PspOMI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI ZraI