Restriction map for
pF1KA0715
(Circular) (Six-base) MAPSORT of: pF1KA0715.seq Check: 4181 from: 1 to: 7496
pF1KA0715 7496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2331 2377 3940 4394 2331
Size: 46 1563 454 5433
Fragments arranged by size:
5433 1563 454 46
AatII G_ACGT'C
Cuts at: 1957 1957
Size: 7496
AccI GT'mk_AC
Cuts at: 2468 3685 4534 2468
Size: 1217 849 5430
Fragments arranged by size:
5430 1217 849
Acc65I G'GTAC_C
Cuts at: 4512 4512
Size: 7496
AclI AA'CG_TT
Cuts at: 7336 7336
Size: 7496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1698 2364 4221 5321 5753 6106 6768 1698
Size: 666 1857 1100 432 353 662 2426
Fragments arranged by size:
2426 1857 1100 666 662 432 353
AflIII A'CryG_T
Cuts at: 756 1205 3467 4848 6648 6929 756
Size: 449 2262 1381 1800 281 1323
Fragments arranged by size:
2262 1800 1381 1323 449 281
AleI CACnn'nnGTG
Cuts at: 2320 2320
Size: 7496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 567 2127 567
Size: 1560 5936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 775 807 775
Size: 32 7464
AlwNI CAG_nnn'CTG
Cuts at: 320 1118 1239 1620 2736 3073 3088 4801 6239
Size: 798 121 381 1116 337 15 1713 1438
Cuts at: 6239 320
Size: 1577
Fragments arranged by size:
1713 1577 1438 1116 798 381 337 121
15
ApaI G_GGCC'C
Cuts at: 1578 1578
Size: 7496
ApaLI G'TGCA_C
Cuts at: 2290 6334 2290
Size: 4044 3452
ApoI r'AATT_y
Cuts at: 566 853 4500 6849 7105 566
Size: 287 3647 2349 256 957
Fragments arranged by size:
3647 2349 957 287 256
AseI AT'TA_AT
Cuts at: 20 2534 20
Size: 2514 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7496
AvaI C'yCGr_G
Cuts at: 1282 1640 2047 4516 1282
Size: 358 407 2469 4262
Fragments arranged by size:
4262 2469 407 358
BamHI G'GATC_C
Cuts at: 1073 1581 2831 4521 1073
Size: 508 1250 1690 4048
Fragments arranged by size:
4048 1690 1250 508
BanI G'GyrC_C
Cuts at: 1644 1682 2365 2441 4512 4926 5174 5209 1644
Size: 38 683 76 2071 414 248 35 3931
Fragments arranged by size:
3931 2071 683 414 248 76 38 35
BanII G_rGCy'C
Cuts at: 1359 1578 1910 2272 2568 3766 4261 4510 5540
Size: 219 332 362 296 1198 495 249 1030
Cuts at: 5540 1359
Size: 3315
Fragments arranged by size:
3315 1198 1030 495 362 332 296 249
219
BbeI G_GCGC'C
Cuts at: 4930 5178 4930
Size: 248 7248
BbsI GAAGACnn'nnnn_
Cuts at: 435 904 2516 2783 3115 3856 435
Size: 469 1612 267 332 741 4075
Fragments arranged by size:
4075 1612 741 469 332 267
BbvCI CC'TCA_GC
Cuts at: 2005 2684 4359 2005
Size: 679 1675 5142
Fragments arranged by size:
5142 1675 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 975 1009 975
Size: 34 7462
BciVI GTATCCnnnnn_n'
Cuts at: 3921 5394 6450 7496 3921
Size: 1473 1056 1046 3921
Fragments arranged by size:
3921 1473 1056 1046
BclI T'GATC_A
Cuts at: 497 1220 5016 497
Size: 723 3796 2977
Fragments arranged by size:
3796 2977 723
BfrBI ATG'CAT
Cuts at: 2104 2621 3712 2104
Size: 517 1091 5888
Fragments arranged by size:
5888 1091 517
BglI GCCn_nnn'nGGC
Cuts at: 2071 2071
Size: 7496
BglII A'GATC_T
Cuts at: 5011 7048 5011
Size: 2037 5459
BlpI GC'TnA_GC
Cuts at: 1490 1613 4608 1490
Size: 123 2995 4378
Fragments arranged by size:
4378 2995 123
Bme1580I G_kGCm'C
Cuts at: 1191 1578 1641 1649 1685 2294 2368 5121 5214
Size: 387 63 8 36 609 74 2753 93
Cuts at: 5214 6338 1191
Size: 1124 2349
Fragments arranged by size:
2753 2349 1124 609 387 93 74 63
36 8
BmgBI CAC'GTC
Cuts at: 757 757
Size: 7496
BmrI ACTGGGnnnn_n'
Cuts at: 1178 2777 3159 4204 4708 4789 5124 7268 1178
Size: 1599 382 1045 504 81 335 2144 1406
Fragments arranged by size:
2144 1599 1406 1045 504 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1536 3895 5863 1536
Size: 2359 1968 3169
Fragments arranged by size:
3169 2359 1968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 279 1216 1519 1727 1981 2200 2758 2873
Size: 91 937 303 208 254 219 558 115
Cuts at: 2873 3190 3501 188
Size: 317 311 4183
Fragments arranged by size:
4183 937 558 317 311 303 254 219
208 115 91
Bpu10I CC'TnA_GC
Cuts at: 2005 2058 2336 2684 3641 4347 4359 2005
Size: 53 278 348 957 706 12 5142
Fragments arranged by size:
5142 957 706 348 278 53 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1320 1920 3373 4401 4672 5551 6265 6563
Size: 590 600 1453 1028 271 879 714 298
Cuts at: 6563 730
Size: 1663
Fragments arranged by size:
1663 1453 1028 879 714 600 590 298
271
BsaI GGTCTCn'nnnn_
Cuts at: 297 1324 1495 1786 2941 7200 297
Size: 1027 171 291 1155 4259 593
Fragments arranged by size:
4259 1155 1027 593 291 171
BsaAI yAC'GTr
Cuts at: 4849 4849
Size: 7496
BsaBI GATnn'nnATC
Cuts at: 6 1078 1246 5010 7047 6
Size: 1072 168 3764 2037 455
Fragments arranged by size:
3764 2037 1072 455 168
BsaHI Gr'CG_yC
Cuts at: 1954 4927 5175 5877 7373 1954
Size: 2973 248 702 1496 2077
Fragments arranged by size:
2973 2077 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2654 4685 5206 5884 6295 6442 6716 2654
Size: 2031 521 678 411 147 274 3434
Fragments arranged by size:
3434 2031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 1384 1414 1620 1650 1783 1813 2173
Size: 30 579 30 206 30 133 30 360
Cuts at: 2173 2203 3793 3823 775
Size: 30 1590 30 4448
Fragments arranged by size:
4448 1590 579 360 206 133 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 664 1638 1641 1830 2221 2377 3572 3871 4347
Size: 974 3 189 391 156 1195 299 476
Cuts at: 4347 4467 664
Size: 120 3693
Fragments arranged by size:
3693 1195 974 476 391 299 189 156
120 3
BseYI C'CCAG_C
Cuts at: 660 2223 2514 2827 4389 4921 5679 6344 6752
Size: 1563 291 313 1562 532 758 665 408
Cuts at: 6752 660
Size: 1404
Fragments arranged by size:
1563 1562 1404 758 665 532 408 313
291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 616 912 2012 2133 2376 2711 306
Size: 310 296 1100 121 243 335 5091
Fragments arranged by size:
5091 1100 335 310 296 243 121
BsiEI CG_ry'CG
Cuts at: 104 5038 5084 6314 104
Size: 4934 46 1230 1286
Fragments arranged by size:
4934 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1614 2294 3881 4261 4385 4447 4510 5288 5478
Size: 680 1587 380 124 62 63 778 190
Cuts at: 5478 6338 1614
Size: 860 2772
Fragments arranged by size:
2772 1587 860 778 680 380 190 124
63 62
BsmI GAATG_Cn'
Cuts at: 3123 3712 3123
Size: 589 6907
BsmBI CGTCTCn'nnnn_
Cuts at: 976 976
Size: 7496
Bsp1286I G_dGCh'C
Cuts at: 1191 1359 1578 1614 1641 1649 1685 1910 2272
Size: 168 219 36 27 8 36 225 362
Cuts at: 2272 2294 2368 2568 3766 3881 4261 4385 4447
Size: 22 74 200 1198 115 380 124 62
Cuts at: 4447 4510 5121 5214 5288 5478 5540 6338 1191
Size: 63 611 93 74 190 62 798 2349
Fragments arranged by size:
2349 1198 798 611 380 362 225 219
200 190 168 124 115 93 74 74
63 62 62 36 36 27 22 8
BspEI T'CCGG_A
Cuts at: 2654 2654
Size: 7496
BspHI T'CATG_A
Cuts at: 494 3251 5928 494
Size: 2757 2677 2062
Fragments arranged by size:
2757 2677 2062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 328 1821 2331 2377 2510 3491 3940 4394 4546
Size: 1493 510 46 133 981 449 454 152
Cuts at: 4546 4760 5062 5443 328
Size: 214 302 381 2381
Fragments arranged by size:
2381 1493 981 510 454 449 381 302
214 152 133 46
BsrBI CCG'CTC
Cuts at: 50 1038 3194 5788 5842 7326 7491 50
Size: 988 2156 2594 54 1484 165 55
Fragments arranged by size:
2594 2156 1484 988 165 55 54
BsrDI GCAATG_nn'
Cuts at: 654 1311 1844 2621 2879 3634 3845 5408 654
Size: 657 533 777 258 755 211 1563 2742
Fragments arranged by size:
2742 1563 777 755 657 533 258 211
BsrFI r'CCGG_y
Cuts at: 1664 2780 4439 5494 5675 1664
Size: 1116 1659 1055 181 3485
Fragments arranged by size:
3485 1659 1116 1055 181
BsrGI T'GTAC_A
Cuts at: 3920 3920
Size: 7496
BssSI C'ACGA_G
Cuts at: 3403 5767 6475 3403
Size: 2364 708 4424
Fragments arranged by size:
4424 2364 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2618 4325 4873 2618
Size: 1707 548 5241
Fragments arranged by size:
5241 1707 548
BstBI TT'CG_AA
Cuts at: 564 794 5857 564
Size: 230 5063 2203
Fragments arranged by size:
5063 2203 230
BstEII G'GTnAC_C
Cuts at: 1920 1920
Size: 7496
BstXI CCAn_nnnn'nTGG
Cuts at: 1272 2235 3444 1272
Size: 963 1209 5324
Fragments arranged by size:
5324 1209 963
BstYI r'GATC_y
Cuts at: 1 1073 1385 1581 2831 4521 4988 5011 5346
Size: 1072 312 196 1250 1690 467 23 335
Cuts at: 5346 5592 5898 5910 5996 7048 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1690 1250 1072 1052 467 449 335 312
306 246 196 86 23 12
BtgI C'CryG_G
Cuts at: 107 434 3867 107
Size: 327 3433 3736
Fragments arranged by size:
3736 3433 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1350 4447 5446 5627 5790 1350
Size: 3097 999 181 163 3056
Fragments arranged by size:
3097 3056 999 181 163
BtsI GCAGTG_nn'
Cuts at: 151 2024 2121 4739 151
Size: 1873 97 2618 2908
Fragments arranged by size:
2908 2618 1873 97
ClaI AT'CG_AT
Cuts at: 3101 3101
Size: 7496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3513 3548 3513
Size: 35 7461
DraI TTT'AAA
Cuts at: 4495 4495
Size: 7496
DraIII CAC_nnn'GTG
Cuts at: 4212 4325 4212
Size: 113 7383
DrdI GACnn_nn'nnGTC
Cuts at: 5202 6546 5202
Size: 1344 6152
EaeI y'GGCC_r
Cuts at: 473 2072 2237 3411 3434 3657 5081 5255 5646
Size: 1599 165 1174 23 223 1424 174 391
Cuts at: 5646 5673 7018 473
Size: 27 1345 951
Fragments arranged by size:
1599 1424 1345 1174 951 391 223 174
165 27 23
EagI C'GGCC_G
Cuts at: 5081 5081
Size: 7496
EarI CTCTTCn'nnn_
Cuts at: 281 346 1322 1526 1984 2413 2639 2975 3205
Size: 65 976 204 458 429 226 336 230
Cuts at: 3205 3493 3568 4040 4283 5519 5729 281
Size: 288 75 472 243 1236 210 2048
Fragments arranged by size:
2048 1236 976 472 458 429 336 288
243 230 226 210 204 75 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5704 6446 6592 7306 5704
Size: 742 146 714 5894
Fragments arranged by size:
5894 742 714 146
EcoICRI GAG'CTC
Cuts at: 4259 4508 4259
Size: 249 7247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 279 1216 1519 1698 1727 1981 2200 2364
Size: 91 937 303 179 29 254 219 164
Cuts at: 2364 2758 2873 3190 3501 4221 5321 5753 6106
Size: 394 115 317 311 720 1100 432 353
Cuts at: 6106 6768 188
Size: 662 916
Fragments arranged by size:
1100 937 916 720 662 432 394 353
317 311 303 254 219 179 164 115
91 29
EcoNI CCTnn'n_nnAGG
Cuts at: 1231 2359 1231
Size: 1128 6368
EcoO109I rG'GnC_Cy
Cuts at: 1127 1415 2220 2844 4393 4635 1127
Size: 288 805 624 1549 242 3988
Fragments arranged by size:
3988 1549 805 624 288 242
EcoRI G'AATT_C
Cuts at: 4500 4500
Size: 7496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5515 5515
Size: 7496
FspI TGC'GCA
Cuts at: 2611 5277 2611
Size: 2666 4830
HaeII r_GCGC'y
Cuts at: 4930 5178 6408 7179 4930
Size: 248 1230 771 5247
Fragments arranged by size:
5247 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 617 649 693 725 1783 1815 2089 2121 2173
Size: 32 44 32 1058 32 274 32 52
Cuts at: 2173 2205 2799 2831 3083 3115 4330 4362 4446
Size: 32 594 32 252 32 1215 32 84
Cuts at: 4446 4478 5338 5368 5370 5400 617
Size: 32 860 30 2 30 2713
Fragments arranged by size:
2713 1215 1058 860 594 274 252 84
52 44 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2877 3480 3686 4535 2877
Size: 603 206 849 5838
Fragments arranged by size:
5838 849 603 206
KasI G'GCGC_C
Cuts at: 4926 5174 4926
Size: 248 7248
KpnI G_GTAC'C
Cuts at: 4516 4516
Size: 7496
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 7496
MluI A'CGCG_T
Cuts at: 6929 6929
Size: 7496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 581 1467 1769 4132 6255 6439 581
Size: 886 302 2363 2123 184 1638
Fragments arranged by size:
2363 2123 1638 886 302 184
MscI TGG'CCA
Cuts at: 475 2074 2239 3413 3436 3659 5257 475
Size: 1599 165 1174 23 223 1598 2714
Fragments arranged by size:
2714 1599 1598 1174 223 165 23
MslI CAynn'nnrTG
Cuts at: 637 946 1084 1456 2109 2320 2536 5485 5612
Size: 309 138 372 653 211 216 2949 127
Cuts at: 5612 637
Size: 2521
Fragments arranged by size:
2949 2521 653 372 309 216 211 138
127
MspA1I CmG'CkG
Cuts at: 195 243 2872 4607 4921 5281 6065 6310 195
Size: 48 2629 1735 314 360 784 245 1381
Fragments arranged by size:
2629 1735 1381 784 360 314 245 48
NaeI GCC'GGC
Cuts at: 5677 5677
Size: 7496
NarI GG'CG_CC
Cuts at: 4927 5175 4927
Size: 248 7248
NcoI C'CATG_G
Cuts at: 107 434 107
Size: 327 7169
NgoMIV G'CCGG_C
Cuts at: 5675 5675
Size: 7496
NsiI A_TGCA'T
Cuts at: 2106 2623 3714 2106
Size: 517 1091 5888
Fragments arranged by size:
5888 1091 517
NspI r_CATG'y
Cuts at: 1209 2160 3342 4285 4549 5049 6652 1209
Size: 951 1182 943 264 500 1603 2053
Fragments arranged by size:
2053 1603 1182 951 943 500 264
PasI CC'CwG_GG
Cuts at: 2361 2397 2361
Size: 36 7460
PciI A'CATG_T
Cuts at: 1205 6648 1205
Size: 5443 2053
PflMI CCAn_nnn'nTGG
Cuts at: 2452 3088 3840 2452
Size: 636 752 6108
Fragments arranged by size:
6108 752 636
PfoI T'CCnGG_A
Cuts at: 295 2177 2326 295
Size: 1882 149 5465
Fragments arranged by size:
5465 1882 149
PmeI GTTT'AAAC
Cuts at: 4495 4495
Size: 7496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 775 807 5942 5974 775
Size: 32 5135 32 2297
Fragments arranged by size:
5135 2297 32 32
PpuMI rG'GwC_Cy
Cuts at: 1127 1415 2844 4393 1127
Size: 288 1429 1549 4230
Fragments arranged by size:
4230 1549 1429 288
PshAI GACnn'nnGTC
Cuts at: 2872 2872
Size: 7496
PspOMI G'GGCC_C
Cuts at: 1574 1574
Size: 7496
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3457 3489 3457
Size: 32 7464
PstI C_TGCA'G
Cuts at: 2789 2896 3621 4543 5228 2789
Size: 107 725 922 685 5057
Fragments arranged by size:
5057 922 725 685 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7496
PvuII CAG'CTG
Cuts at: 4921 5281 4921
Size: 360 7136
RsrII CG'GwC_CG
Cuts at: 3176 5691 3176
Size: 2515 4981
SacI G_AGCT'C
Cuts at: 4261 4510 4261
Size: 249 7247
SalI G'TCGA_C
Cuts at: 3684 4533 3684
Size: 849 6647
SanDI GG'GwC_CC
Cuts at: 1415 1415
Size: 7496
SapI GCTCTTCn'nnn_
Cuts at: 1984 2975 3493 4040 5519 5729 1984
Size: 991 518 547 1479 210 3751
Fragments arranged by size:
3751 1479 991 547 518 210
SbfI CC_TGCA'GG
Cuts at: 4543 4543
Size: 7496
ScaI AGT'ACT
Cuts at: 4298 7013 4298
Size: 2715 4781
SfcI C'TryA_G
Cuts at: 33 1103 2145 2307 2785 2892 3004 3617 4539
Size: 1070 1042 162 478 107 112 613 922
Cuts at: 4539 5224 6192 6383 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1070 1042 968 922 685 613 478
191 162 112 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 2071 2071
Size: 7496
SfoI GGC'GCC
Cuts at: 4928 5176 4928
Size: 248 7248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7496
SmaI CCC'GGG
Cuts at: 1642 4518 1642
Size: 2876 4620
SmlI C'TyrA_G
Cuts at: 745 1299 1935 3388 4416 4651 5566 6280 6542
Size: 554 636 1453 1028 235 915 714 262
Cuts at: 6542 745
Size: 1699
Fragments arranged by size:
1699 1453 1028 915 714 636 554 262
235
SpeI A'CTAG_T
Cuts at: 67 2319 67
Size: 2252 5244
SphI G_CATG'C
Cuts at: 3342 4549 5049 3342
Size: 1207 500 5789
Fragments arranged by size:
5789 1207 500
StuI AGG'CCT
Cuts at: 2244 4170 2244
Size: 1926 5570
StyI C'CwwG_G
Cuts at: 107 434 1191 1763 1817 2201 2474 4630 4984
Size: 327 757 572 54 384 273 2156 354
Cuts at: 4984 107
Size: 2619
Fragments arranged by size:
2619 2156 757 572 384 354 327 273
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1348 2459 2478 2926 3164 3957 4223 5079 5212
Size: 1111 19 448 238 793 266 856 133
Cuts at: 5212 5671 5880 1348
Size: 459 209 2964
Fragments arranged by size:
2964 1111 856 793 459 448 266 238
209 133 19
TatI w'GTAC_w
Cuts at: 1391 3449 3899 3920 4296 7011 1391
Size: 2058 450 21 376 2715 1876
Fragments arranged by size:
2715 2058 1876 450 376 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 115 147 1620 1652 7013 7045 115
Size: 32 1473 32 5361 32 566
Fragments arranged by size:
5361 1473 566 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1797 2410 2488 5293 1797
Size: 613 78 2805 4000
Fragments arranged by size:
4000 2805 613 78
XbaI T'CTAG_A
Cuts at: 2583 4527 2583
Size: 1944 5552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 889 4991 468
Size: 421 4102 2973
Fragments arranged by size:
4102 2973 421
XmaI C'CCGG_G
Cuts at: 1640 4516 1640
Size: 2876 4620
XmnI GAAnn'nnTTC
Cuts at: 769 2795 769
Size: 2026 5470
ZraI GAC'GTC
Cuts at: 1955 1955
Size: 7496
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AvrII BaeI BmtI
BsiWI BssHII BstZ17I Bsu36I EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PmlI PsiI
PspXI SacII SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI