Restriction map for
pF1KA0720
(Circular) (Six-base) MAPSORT of: pF1KA0720.seq Check: 3824 from: 1 to: 6299
pF1KA0720 6299 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2900 2900
Size: 6299
AccI GT'mk_AC
Cuts at: 1076 3337 1076
Size: 2261 4038
Acc65I G'GTAC_C
Cuts at: 3315 3315
Size: 6299
AclI AA'CG_TT
Cuts at: 6139 6139
Size: 6299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1720 1981 2447 4124 4556 5303 5571 1008
Size: 712 261 466 1677 432 747 268 1736
Fragments arranged by size:
1736 1677 747 712 466 432 268 261
AfeI AGC'GCT
Cuts at: 1517 1834 1517
Size: 317 5982
AflIII A'CryG_T
Cuts at: 338 890 1853 2261 3651 4755 5732 338
Size: 552 963 408 1390 1104 977 905
Fragments arranged by size:
1390 1104 977 963 905 552 408
AgeI A'CCGG_T
Cuts at: 4687 4687
Size: 6299
AhdI GACnn_n'nnGTC
Cuts at: 768 2208 2583 768
Size: 1440 375 4484
Fragments arranged by size:
4484 1440 375
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1408 1440 1408
Size: 32 6267
AlwNI CAG_nnn'CTG
Cuts at: 216 1698 1887 2031 2376 2385 2931 2941 3604
Size: 1482 189 144 345 9 546 10 663
Cuts at: 3604 5171 216
Size: 1567 1344
Fragments arranged by size:
1567 1482 1344 663 546 345 189 144
10 9
ApaI G_GGCC'C
Cuts at: 233 812 2805 3050 3072 233
Size: 579 1993 245 22 3460
Fragments arranged by size:
3460 1993 579 245 22
ApaLI G'TGCA_C
Cuts at: 2345 5069 2345
Size: 2724 3575
ApoI r'AATT_y
Cuts at: 514 1966 3303 5652 5908 514
Size: 1452 1337 2349 256 905
Fragments arranged by size:
2349 1452 1337 905 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6299
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6299
AvaI C'yCGr_G
Cuts at: 922 1142 1528 2593 2798 3159 3319 922
Size: 220 386 1065 205 361 160 3902
Fragments arranged by size:
3902 1065 386 361 220 205 160
BamHI G'GATC_C
Cuts at: 3324 3324
Size: 6299
BanI G'GyrC_C
Cuts at: 1482 2002 2734 2944 3315 3729 3977 4012 1482
Size: 520 732 210 371 414 248 35 3769
Fragments arranged by size:
3769 732 520 414 371 248 210 35
BanII G_rGCy'C
Cuts at: 124 233 409 812 935 1244 2357 2805 2990
Size: 109 176 403 123 309 1113 448 185
Cuts at: 2990 3031 3050 3072 3191 3313 4343 124
Size: 41 19 22 119 122 1030 2080
Fragments arranged by size:
2080 1113 1030 448 403 309 185 176
123 122 119 109 41 22 19
BbeI G_GCGC'C
Cuts at: 1486 2006 3733 3981 1486
Size: 520 1727 248 3804
Fragments arranged by size:
3804 1727 520 248
BbsI GAAGACnn'nnnn_
Cuts at: 1196 1783 1196
Size: 587 5712
BbvCI CC'TCA_GC
Cuts at: 1771 1771
Size: 6299
BciVI GTATCCnnnnn_n'
Cuts at: 4197 4958 6299 4197
Size: 761 1341 4197
Fragments arranged by size:
4197 1341 761
BclI T'GATC_A
Cuts at: 1346 3819 1346
Size: 2473 3826
BfrBI ATG'CAT
Cuts at: 279 279
Size: 6299
BglI GCCn_nnn'nGGC
Cuts at: 1146 1801 1146
Size: 655 5644
BglII A'GATC_T
Cuts at: 3814 5851 3814
Size: 2037 4262
BlpI GC'TnA_GC
Cuts at: 1705 3411 1705
Size: 1706 4593
Bme1580I G_kGCm'C
Cuts at: 233 812 2349 2805 3050 3072 3924 4017 5073
Size: 579 1537 456 245 22 852 93 1056
Cuts at: 5073 233
Size: 1459
Fragments arranged by size:
1537 1459 1056 852 579 456 245 93
22
BmrI ACTGGGnnnn_n'
Cuts at: 583 2616 3511 3592 3927 6071 583
Size: 2033 895 81 335 2144 811
Fragments arranged by size:
2144 2033 895 811 335 81
BmtI G_CTAG'C
Cuts at: 1475 1475
Size: 6299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 423 4666 423
Size: 4243 2056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 841 930 1114 1243 1513 2056 2908 749
Size: 92 89 184 129 270 543 852 4140
Fragments arranged by size:
4140 852 543 270 184 129 92 89
Bpu10I CC'TnA_GC
Cuts at: 1771 3185 1771
Size: 1414 4885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1744 2881 3475 4354 4846 5144 1744
Size: 1137 594 879 492 298 2899
Fragments arranged by size:
2899 1137 879 594 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2539 2626 3143 3207 6003 2539
Size: 87 517 64 2796 2835
Fragments arranged by size:
2835 2796 517 87 64
BsaAI yAC'GTr
Cuts at: 1667 1848 3652 1667
Size: 181 1804 4314
Fragments arranged by size:
4314 1804 181
BsaBI GATnn'nnATC
Cuts at: 6 3813 5850 6
Size: 3807 2037 455
Fragments arranged by size:
3807 2037 455
BsaHI Gr'CG_yC
Cuts at: 833 1483 1913 2003 3730 3978 4680 6176 833
Size: 650 430 90 1727 248 702 1496 956
Fragments arranged by size:
1727 1496 956 702 650 430 248 90
BsaWI w'CCGG_w
Cuts at: 428 2694 3488 4009 4687 4961 5108 5519 428
Size: 2266 794 521 678 274 147 411 1208
Fragments arranged by size:
2266 1208 794 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1150 1165 1180 1195 1282 1312 1408 1438 2692
Size: 15 15 15 87 30 96 30 1254
Cuts at: 2692 2722 1150
Size: 30 4727
Fragments arranged by size:
4727 1254 96 87 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 406 409 412 415 486 1189 1192 1612
Size: 3 3 3 3 71 703 3 420
Cuts at: 1612 1615 1798 2029 2407 2410 2425 2434 2437
Size: 3 183 231 378 3 15 9 3
Cuts at: 2437 2440 2443 2446 2449 2452 2911 403
Size: 3 3 3 3 3 459 3791
Fragments arranged by size:
3791 703 459 420 378 231 183 71
15 9 3 3 3 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 552 618 717 799 961 1048 2297 2968 3043
Size: 66 99 82 162 87 1249 671 75
Cuts at: 3043 3049 3053 3209 3233 3724 4482 5059 5555
Size: 6 4 156 24 491 758 577 496
Cuts at: 5555 552
Size: 1296
Fragments arranged by size:
1296 1249 758 671 577 496 491 162
156 99 87 82 75 66 24 6
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1090 1294 1438 1957 202
Size: 888 204 144 519 4544
Fragments arranged by size:
4544 888 519 204 144
BsiEI CG_ry'CG
Cuts at: 104 1895 2706 3841 3887 5095 104
Size: 1791 811 1135 46 1208 1308
Fragments arranged by size:
1791 1308 1208 1135 811 46
BsiHKAI G_wGCw'C
Cuts at: 409 439 1244 2349 2990 3313 4091 4281 5073
Size: 30 805 1105 641 323 778 190 792
Cuts at: 5073 409
Size: 1635
Fragments arranged by size:
1635 1105 805 792 778 641 323 190
30
BsmI GAATG_Cn'
Cuts at: 505 505
Size: 6299
BsmBI CGTCTCn'nnnn_
Cuts at: 2011 2851 2011
Size: 840 5459
Bsp1286I G_dGCh'C
Cuts at: 124 233 409 439 812 935 1244 2349 2357
Size: 109 176 30 373 123 309 1105 8
Cuts at: 2357 2805 2990 3031 3050 3072 3191 3313 3924
Size: 448 185 41 19 22 119 122 611
Cuts at: 3924 4017 4091 4281 4343 5073 124
Size: 93 74 190 62 730 1350
Fragments arranged by size:
1350 1105 730 611 448 373 309 190
185 176 123 122 119 109 93 74
62 41 30 22 19 8
BspEI T'CCGG_A
Cuts at: 5519 5519
Size: 6299
BspHI T'CATG_A
Cuts at: 5475 5475
Size: 6299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1388 2900 3129 3161 3349 3563 3865 4246 1388
Size: 1512 229 32 188 214 302 381 3441
Fragments arranged by size:
3441 1512 381 302 229 214 188 32
BsrBI CCG'CTC
Cuts at: 50 1878 2708 4591 4645 6129 6294 50
Size: 1828 830 1883 54 1484 165 55
Fragments arranged by size:
1883 1828 1484 830 165 55 54
BsrDI GCAATG_nn'
Cuts at: 611 4211 611
Size: 3600 2699
BsrFI r'CCGG_y
Cuts at: 1485 4297 4478 4687 1485
Size: 2812 181 209 3097
Fragments arranged by size:
3097 2812 209 181
BssHII G'CGCG_C
Cuts at: 1791 1791
Size: 6299
BssSI C'ACGA_G
Cuts at: 2170 2816 4570 4928 2170
Size: 646 1754 358 3541
Fragments arranged by size:
3541 1754 646 358
BstAPI GCAn_nnn'nTGC
Cuts at: 500 2031 2340 2376 3676 500
Size: 1531 309 36 1300 3123
Fragments arranged by size:
3123 1531 1300 309 36
BstBI TT'CG_AA
Cuts at: 4660 4660
Size: 6299
BstEII G'GTnAC_C
Cuts at: 2115 2115
Size: 6299
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 2562 2275
Size: 287 6012
BstYI r'GATC_y
Cuts at: 1 2103 3324 3791 3814 4149 4395 5407 5493
Size: 2102 1221 467 23 335 246 1012 86
Cuts at: 5493 5505 5851 1
Size: 12 346 449
Fragments arranged by size:
2102 1221 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1216 2047 3035 1216
Size: 831 988 4480
Fragments arranged by size:
4480 988 831
BtgI C'CryG_G
Cuts at: 107 1826 1895 2292 2540 2555 2717 2949 107
Size: 1719 69 397 248 15 162 232 3457
Fragments arranged by size:
3457 1719 397 248 232 162 69 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4249 4430 4593 4249
Size: 181 163 5955
Fragments arranged by size:
5955 181 163
BtsI GCAGTG_nn'
Cuts at: 383 978 1282 2132 2648 3278 3542 383
Size: 595 304 850 516 630 264 3140
Fragments arranged by size:
3140 850 630 595 516 304 264
DraI TTT'AAA
Cuts at: 3298 3298
Size: 6299
DraIII CAC_nnn'GTG
Cuts at: 1848 1848
Size: 6299
DrdI GACnn_nn'nnGTC
Cuts at: 146 4005 4863 146
Size: 3859 858 1582
Fragments arranged by size:
3859 1582 858
EaeI y'GGCC_r
Cuts at: 182 793 873 1013 1065 1718 1892 2277 2552
Size: 611 80 140 52 653 174 385 275
Cuts at: 2552 2703 3110 3884 4058 4449 4476 5821 182
Size: 151 407 774 174 391 27 1345 660
Fragments arranged by size:
1345 774 660 653 611 407 391 385
275 174 174 151 140 80 52 27
EagI C'GGCC_G
Cuts at: 1892 2703 3884 1892
Size: 811 1181 4307
Fragments arranged by size:
4307 1181 811
EarI CTCTTCn'nnn_
Cuts at: 427 1126 1573 1654 2101 2384 2411 3133 4322
Size: 699 447 81 447 283 27 722 1189
Cuts at: 4322 4532 427
Size: 210 2194
Fragments arranged by size:
2194 1189 722 699 447 447 283 210
81 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1429 1687 2839 4507 4817 4963 6109 1429
Size: 258 1152 1668 310 146 1146 1619
Fragments arranged by size:
1668 1619 1152 1146 310 258 146
EcoICRI GAG'CTC
Cuts at: 407 1242 2988 3311 407
Size: 835 1746 323 3395
Fragments arranged by size:
3395 1746 835 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 841 930 1008 1114 1243 1513 1720 1981
Size: 92 89 78 106 129 270 207 261
Cuts at: 1981 2056 2447 2908 4124 4556 5303 5571 749
Size: 75 391 461 1216 432 747 268 1477
Fragments arranged by size:
1477 1216 747 461 432 391 270 268
261 207 129 106 92 89 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 244 907 1215 1329 2389 3171 244
Size: 663 308 114 1060 782 3372
Fragments arranged by size:
3372 1060 782 663 308 114
EcoO109I rG'GnC_Cy
Cuts at: 229 230 809 2202 2210 2255 2802 3068 3069
Size: 1 579 1393 8 45 547 266 1
Cuts at: 3069 3164 3438 229
Size: 95 274 3090
Fragments arranged by size:
3090 1393 579 547 274 266 95 45
8 1 1
EcoRI G'AATT_C
Cuts at: 3303 3303
Size: 6299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 519 4318 519
Size: 3799 2500
FspI TGC'GCA
Cuts at: 4080 4080
Size: 6299
HaeII r_GCGC'y
Cuts at: 1061 1422 1486 1519 1836 1993 2006 3733 3981
Size: 361 64 33 317 157 13 1727 248
Cuts at: 3981 5003 5982 1061
Size: 1022 979 1378
Fragments arranged by size:
1727 1378 1022 979 361 317 248 157
64 33 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 1150 1182 2194 2226 2569 2601 2620
Size: 32 364 32 1012 32 343 32 19
Cuts at: 2620 2652 2692 2724 3142 3174 4141 4171 4173
Size: 32 40 32 418 32 967 30 2
Cuts at: 4173 4203 754
Size: 30 2850
Fragments arranged by size:
2850 1012 967 418 364 343 40 32
32 32 32 32 32 32 30 30
19 2
HincII GTy'rAC
Cuts at: 3338 3338
Size: 6299
KasI G'GCGC_C
Cuts at: 1482 2002 3729 3977 1482
Size: 520 1727 248 3804
Fragments arranged by size:
3804 1727 520 248
KpnI G_GTAC'C
Cuts at: 3319 3319
Size: 6299
MluI A'CGCG_T
Cuts at: 1853 5732 1853
Size: 3879 2420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 875 4970 5154 674
Size: 201 4095 184 1819
Fragments arranged by size:
4095 1819 201 184
MscI TGG'CCA
Cuts at: 1720 2279 2554 4060 1720
Size: 559 275 1506 3959
Fragments arranged by size:
3959 1506 559 275
MslI CAynn'nnrTG
Cuts at: 284 482 2560 4288 4415 284
Size: 198 2078 1728 127 2168
Fragments arranged by size:
2168 2078 1728 198 127
MspA1I CmG'CkG
Cuts at: 127 216 799 950 965 998 1028 1092 1232
Size: 89 583 151 15 33 30 64 140
Cuts at: 1232 1452 1734 1752 1869 1884 2028 2331 2340
Size: 220 282 18 117 15 144 303 9
Cuts at: 2340 2467 2501 2606 2871 2928 3053 3086 3410
Size: 127 34 105 265 57 125 33 324
Cuts at: 3410 3724 4084 5097 5342 127
Size: 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 583 360 324 314 303 282
265 245 220 151 144 140 127 125
117 105 89 64 57 34 33 33
30 18 15 15 9
NaeI GCC'GGC
Cuts at: 4480 4480
Size: 6299
NarI GG'CG_CC
Cuts at: 1483 2003 3730 3978 1483
Size: 520 1727 248 3804
Fragments arranged by size:
3804 1727 520 248
NcoI C'CATG_G
Cuts at: 107 2555 2717 2949 107
Size: 2448 162 232 3457
Fragments arranged by size:
3457 2448 232 162
NgoMIV G'CCGG_C
Cuts at: 4478 4478
Size: 6299
NheI G'CTAG_C
Cuts at: 1471 1471
Size: 6299
NsiI A_TGCA'T
Cuts at: 281 281
Size: 6299
NspI r_CATG'y
Cuts at: 295 894 1623 1716 1863 3352 3852 4759 295
Size: 599 729 93 147 1489 500 907 1835
Fragments arranged by size:
1835 1489 907 729 599 500 147 93
PasI CC'CwG_GG
Cuts at: 2206 2688 3173 2206
Size: 482 485 5332
Fragments arranged by size:
5332 485 482
PciI A'CATG_T
Cuts at: 890 4755 890
Size: 3865 2434
PflMI CCAn_nnn'nTGG
Cuts at: 2931 2931
Size: 6299
PfoI T'CCnGG_A
Cuts at: 1436 1436
Size: 6299
PmeI GTTT'AAAC
Cuts at: 3298 3298
Size: 6299
PmlI CAC'GTG
Cuts at: 1667 1848 1667
Size: 181 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1408 1440 5438 5470 1408
Size: 32 3998 32 2237
Fragments arranged by size:
3998 2237 32 32
PpuMI rG'GwC_Cy
Cuts at: 2202 2210 2202
Size: 8 6291
PshAI GACnn'nnGTC
Cuts at: 2149 2606 2149
Size: 457 5842
PspOMI G'GGCC_C
Cuts at: 229 808 2801 3046 3068 229
Size: 579 1993 245 22 3460
Fragments arranged by size:
3460 1993 579 245 22
PstI C_TGCA'G
Cuts at: 2381 3126 3346 4031 2381
Size: 745 220 685 4649
Fragments arranged by size:
4649 745 685 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6299
PvuII CAG'CTG
Cuts at: 216 799 950 998 1092 1232 1452 2028 2331
Size: 583 151 48 94 140 220 576 303
Cuts at: 2331 2340 2467 2871 2928 3053 3724 4084 216
Size: 9 127 404 57 125 671 360 2431
Fragments arranged by size:
2431 671 583 576 404 360 303 220
151 140 127 125 94 57 48 9
RsrII CG'GwC_CG
Cuts at: 4494 4494
Size: 6299
SacI G_AGCT'C
Cuts at: 409 1244 2990 3313 409
Size: 835 1746 323 3395
Fragments arranged by size:
3395 1746 835 323
SalI G'TCGA_C
Cuts at: 3336 3336
Size: 6299
SanDI GG'GwC_CC
Cuts at: 2202 2202
Size: 6299
SapI GCTCTTCn'nnn_
Cuts at: 427 1573 4322 4532 427
Size: 1146 2749 210 2194
Fragments arranged by size:
2749 2194 1146 210
SbfI CC_TGCA'GG
Cuts at: 3126 3346 3126
Size: 220 6079
ScaI AGT'ACT
Cuts at: 5816 5816
Size: 6299
SfcI C'TryA_G
Cuts at: 33 681 1113 1522 2249 2377 3122 3342 4027
Size: 648 432 409 727 128 745 220 685
Cuts at: 4027 5020 5211 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 745 727 685 648 432 409
220 191 128
SfoI GGC'GCC
Cuts at: 1484 2004 3731 3979 1484
Size: 520 1727 248 3804
Fragments arranged by size:
3804 1727 520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6299
SgrAI Cr'CCGG_yG
Cuts at: 1485 1485
Size: 6299
SmaI CCC'GGG
Cuts at: 924 1144 1530 3321 924
Size: 220 386 1791 3902
Fragments arranged by size:
3902 1791 386 220
SmlI C'TyrA_G
Cuts at: 1759 2896 3454 4369 4861 5123 1759
Size: 1137 558 915 492 262 2935
Fragments arranged by size:
2935 1137 915 558 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6299
SphI G_CATG'C
Cuts at: 1863 3352 3852 1863
Size: 1489 500 4310
Fragments arranged by size:
4310 1489 500
StuI AGG'CCT
Cuts at: 250 443 679 1321 2284 250
Size: 193 236 642 963 4265
Fragments arranged by size:
4265 963 642 236 193
StyI C'CwwG_G
Cuts at: 107 185 1796 2555 2717 2949 3433 3787 107
Size: 78 1611 759 162 232 484 354 2619
Fragments arranged by size:
2619 1611 759 484 354 232 162 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1697 1795 2289 2962 3882 4015 4474 4683 1697
Size: 98 494 673 920 133 459 209 3313
Fragments arranged by size:
3313 920 673 494 459 209 133 98
TatI w'GTAC_w
Cuts at: 1616 2084 5814 1616
Size: 468 3730 2101
Fragments arranged by size:
3730 2101 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1282 1314 3129 3161 5816 5848 1282
Size: 32 1815 32 2655 32 1733
Fragments arranged by size:
2655 1815 1733 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4096 4096
Size: 6299
XbaI T'CTAG_A
Cuts at: 3330 3330
Size: 6299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 2547 3794 375
Size: 2172 1247 2880
Fragments arranged by size:
2880 2172 1247
XmaI C'CCGG_G
Cuts at: 922 1142 1528 3319 922
Size: 220 386 1791 3902
Fragments arranged by size:
3902 1791 386 220
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AscI AvrII BaeI BcgI
BmgBI BsiWI BsrGI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI PacI
PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI