Restriction map for
pF1KA0721
(Circular) (Six-base) MAPSORT of: pF1KA0721.seq Check: 4379 from: 1 to: 4355
pF1KA0721 4355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 308 1393 308
Size: 1085 3270
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1317 2180 2612 3359 3627 769
Size: 548 863 432 747 268 1497
Fragments arranged by size:
1497 863 747 548 432 268
AflIII A'CryG_T
Cuts at: 1707 2811 3788 1707
Size: 1104 977 2274
Fragments arranged by size:
2274 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 398 398
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 418 1660 3227 418
Size: 1242 1567 1546
Fragments arranged by size:
1567 1546 1242
ApaI G_GGCC'C
Cuts at: 699 1280 699
Size: 581 3774
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 991 1201 1289 1359 3708 3964 991
Size: 210 88 70 2349 256 1382
Fragments arranged by size:
2349 1382 256 210 88 70
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 300 1380 300
Size: 1080 3275
BanI G'GyrC_C
Cuts at: 1103 1371 1785 2033 2068 1103
Size: 268 414 248 35 3390
Fragments arranged by size:
3390 414 268 248 35
BanII G_rGCy'C
Cuts at: 215 473 699 1280 1369 2399 215
Size: 258 226 581 89 1030 2171
Fragments arranged by size:
2171 1030 581 258 226 89
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 4355
BbvCI CC'TCA_GC
Cuts at: 934 934
Size: 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 185 219 1195 1229 185
Size: 34 976 34 3311
Fragments arranged by size:
3311 976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 908 944 1875 908
Size: 36 931 3388
Fragments arranged by size:
3388 931 36
BglI GCCn_nnn'nGGC
Cuts at: 427 586 427
Size: 159 4196
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 699 1280 1980 2073 3129 699
Size: 581 700 93 1056 1925
Fragments arranged by size:
1925 1056 700 581 93
BmrI ACTGGGnnnn_n'
Cuts at: 328 1567 1648 1983 4127 328
Size: 1239 81 335 2144 556
Fragments arranged by size:
2144 1239 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 748 289
Size: 459 3896
Bpu10I CC'TnA_GC
Cuts at: 934 934
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1531 2410 2902 3200 811
Size: 720 879 492 298 1966
Fragments arranged by size:
1966 879 720 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 191 265 533 4059 191
Size: 74 268 3526 487
Fragments arranged by size:
3526 487 268 74
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 189 1544 2065 2743 3017 3164 3575 189
Size: 1355 521 678 274 147 411 969
Fragments arranged by size:
1355 969 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 694 976 694
Size: 282 4073
BseYI C'CCAG_C
Cuts at: 484 893 1780 2538 3115 3611 484
Size: 409 887 758 577 496 1228
Fragments arranged by size:
1228 887 758 577 496 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 664 377
Size: 287 4068
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1369 2147 2337 3129 1369
Size: 778 190 792 2595
Fragments arranged by size:
2595 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 215 473 699 1280 1369 1980 2073 2147 2337
Size: 258 226 581 89 611 93 74 190
Cuts at: 2337 2399 3129 215
Size: 62 730 1441
Fragments arranged by size:
1441 730 611 581 258 226 190 93
89 74 62
BspEI T'CCGG_A
Cuts at: 189 3575 189
Size: 3386 969
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 2647 2701 4185 4350 50
Size: 63 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 151 2353 2534 2743 151
Size: 2202 181 209 1763
Fragments arranged by size:
2202 1763 209 181
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 418 1732 418
Size: 1314 3041
BstBI TT'CG_AA
Cuts at: 882 2716 882
Size: 1834 2521
BstXI CCAn_nnnn'nTGG
Cuts at: 451 866 1100 451
Size: 415 234 3706
Fragments arranged by size:
3706 415 234
BstYI r'GATC_y
Cuts at: 1 186 300 1060 1327 1380 1847 1870 2205
Size: 185 114 760 267 53 467 23 335
Cuts at: 2205 2451 3463 3549 3561 3907 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 760 467 449 346 335 267 246
185 114 86 53 23 12
Bsu36I CC'TnA_GG
Cuts at: 180 180
Size: 4355
BtgI C'CryG_G
Cuts at: 474 474
Size: 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 637 2305 2486 2649 637
Size: 1668 181 163 2343
Fragments arranged by size:
2343 1668 181 163
BtsI GCAGTG_nn'
Cuts at: 599 1598 599
Size: 999 3356
ClaI AT'CG_AT
Cuts at: 737 737
Size: 4355
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 804 915 1068 1940 2114 2505 2532 3877 804
Size: 111 153 872 174 391 27 1345 1282
Fragments arranged by size:
1345 1282 872 391 174 153 111 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 748 769 1317 2180 2612 3359 3627 289
Size: 459 21 548 863 432 747 268 1017
Fragments arranged by size:
1017 863 747 548 459 432 268 21
EcoO109I rG'GnC_Cy
Cuts at: 695 696 776 1276 1277 1494 695
Size: 1 80 500 1 217 3556
Fragments arranged by size:
3556 500 217 80 1 1
EcoRI G'AATT_C
Cuts at: 1201 1289 1359 1201
Size: 88 70 4197
Fragments arranged by size:
4197 88 70
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1020 2374 1020
Size: 1354 3001
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1325 1789 2037 3059 4038 1325
Size: 464 248 1022 979 1642
Fragments arranged by size:
1642 1022 979 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 2197 2227 2229 2259 163
Size: 32 2002 30 2 30 2259
Fragments arranged by size:
2259 2002 32 30 30 2
HincII GTy'rAC
Cuts at: 309 1394 309
Size: 1085 3270
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 917 2116 917
Size: 1199 3156
MslI CAynn'nnrTG
Cuts at: 2344 2471 2344
Size: 127 4228
MspA1I CmG'CkG
Cuts at: 897 1100 1466 1780 2140 3153 3398 897
Size: 203 366 314 360 1013 245 1854
Fragments arranged by size:
1854 1013 366 360 314 245 203
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NspI r_CATG'y
Cuts at: 813 828 933 1408 1908 2815 813
Size: 15 105 475 500 907 2353
Fragments arranged by size:
2353 907 500 475 105 15
PasI CC'CwG_GG
Cuts at: 692 692
Size: 4355
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PspOMI G'GGCC_C
Cuts at: 695 1276 695
Size: 581 3774
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 897 1780 2140 897
Size: 883 360 3112
Fragments arranged by size:
3112 883 360
RsrII CG'GwC_CG
Cuts at: 192 2550 192
Size: 2358 1997
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 307 1392 307
Size: 1085 3270
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 1398 2083 3076 3267 33
Size: 1365 685 993 191 1121
Fragments arranged by size:
1365 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 790 1510 2425 2917 3179 790
Size: 720 915 492 262 1966
Fragments arranged by size:
1966 915 720 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 813 1408 1908 813
Size: 595 500 3260
Fragments arranged by size:
3260 595 500
StyI C'CwwG_G
Cuts at: 207 554 1303 1489 1843 207
Size: 347 749 186 354 2719
Fragments arranged by size:
2719 749 354 347 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 1258 1938 2071 2530 2739 209
Size: 1049 680 133 459 209 1825
Fragments arranged by size:
1825 1049 680 459 209 133
TatI w'GTAC_w
Cuts at: 3870 3870
Size: 4355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4323
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 498 1197 1386 498
Size: 699 189 3467
Fragments arranged by size:
3467 699 189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1850 114
Size: 1736 2619
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI