Restriction map for
pF1KA0725
(Circular) (Six-base) MAPSORT of: pF1KA0725.seq Check: 4058 from: 1 to: 5246
pF1KA0725 5246 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1557 1557
Size: 5246
AccI GT'mk_AC
Cuts at: 410 675 2284 410
Size: 265 1609 3372
Fragments arranged by size:
3372 1609 265
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5246
AclI AA'CG_TT
Cuts at: 5086 5086
Size: 5246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 1282 1363 1466 1925 1967 1982 2189 3071
Size: 796 81 103 459 42 15 207 882
Cuts at: 3071 3503 3856 4518 486
Size: 432 353 662 1214
Fragments arranged by size:
1214 882 796 662 459 432 353 207
103 81 42 15
AflII C'TTAA_G
Cuts at: 1864 1864
Size: 5246
AflIII A'CryG_T
Cuts at: 2598 4398 4679 2598
Size: 1800 281 3165
Fragments arranged by size:
3165 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 306 640 1972 2551 3989 306
Size: 334 1332 579 1438 1563
Fragments arranged by size:
1563 1438 1332 579 334
ApaLI G'TGCA_C
Cuts at: 4084 4084
Size: 5246
ApoI r'AATT_y
Cuts at: 904 1309 1391 1774 2250 4599 4855 904
Size: 405 82 383 476 2349 256 1295
Fragments arranged by size:
2349 1295 476 405 383 256 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 5246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
AvaI C'yCGr_G
Cuts at: 2266 2266
Size: 5246
BamHI G'GATC_C
Cuts at: 2271 2271
Size: 5246
BanI G'GyrC_C
Cuts at: 2262 2676 2924 2959 2262
Size: 414 248 35 4549
Fragments arranged by size:
4549 414 248 35
BanII G_rGCy'C
Cuts at: 1920 2260 3290 1920
Size: 340 1030 3876
Fragments arranged by size:
3876 1030 340
BbeI G_GCGC'C
Cuts at: 2680 2928 2680
Size: 248 4998
BbsI GAAGACnn'nnnn_
Cuts at: 1020 1020
Size: 5246
BciVI GTATCCnnnnn_n'
Cuts at: 1152 3144 4200 5246 1152
Size: 1992 1056 1046 1152
Fragments arranged by size:
1992 1152 1056 1046
BclI T'GATC_A
Cuts at: 2226 2766 2226
Size: 540 4706
BglII A'GATC_T
Cuts at: 957 1374 2198 2761 4798 957
Size: 417 824 563 2037 1405
Fragments arranged by size:
2037 1405 824 563 417
BlpI GC'TnA_GC
Cuts at: 2358 2358
Size: 5246
Bme1580I G_kGCm'C
Cuts at: 2871 2964 4088 2871
Size: 93 1124 4029
Fragments arranged by size:
4029 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 138 423 2169 2458 2539 2874 5018 138
Size: 285 1746 289 81 335 2144 366
Fragments arranged by size:
2144 1746 366 335 289 285 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 726 3613 726
Size: 2887 2359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2422 3301 4015 4313 2422
Size: 879 714 298 3355
Fragments arranged by size:
3355 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1455 2008 4950 1455
Size: 553 2942 1751
Fragments arranged by size:
2942 1751 553
BsaAI yAC'GTr
Cuts at: 449 2599 449
Size: 2150 3096
BsaBI GATnn'nnATC
Cuts at: 6 956 2760 4797 6
Size: 950 1804 2037 455
Fragments arranged by size:
2037 1804 950 455
BsaHI Gr'CG_yC
Cuts at: 2677 2925 3627 5123 2677
Size: 248 702 1496 2800
Fragments arranged by size:
2800 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2435 2956 3634 4045 4192 4466 2435
Size: 521 678 411 147 274 3215
Fragments arranged by size:
3215 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 1902 1932 124
Size: 30 1748 30 3438
Fragments arranged by size:
3438 1748 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 710 710
Size: 5246
BseYI C'CCAG_C
Cuts at: 979 2671 3429 4094 4502 979
Size: 1692 758 665 408 1723
Fragments arranged by size:
1723 1692 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 5246
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4064 104
Size: 2684 46 1230 1286
Fragments arranged by size:
2684 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1920 2260 3038 3228 4088 1920
Size: 340 778 190 860 3078
Fragments arranged by size:
3078 860 778 340 190
BsmI GAATG_Cn'
Cuts at: 936 936
Size: 5246
BsmBI CGTCTCn'nnnn_
Cuts at: 432 432
Size: 5246
Bsp1286I G_dGCh'C
Cuts at: 1920 2260 2871 2964 3038 3228 3290 4088 1920
Size: 340 611 93 74 190 62 798 3078
Fragments arranged by size:
3078 798 611 340 190 93 74 62
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1557 2296 2510 2812 3193 1557
Size: 739 214 302 381 3610
Fragments arranged by size:
3610 739 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3538 3592 5076 5241 50
Size: 3488 54 1484 165 55
Fragments arranged by size:
3488 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1145 3158 1145
Size: 2013 3233
BsrFI r'CCGG_y
Cuts at: 3244 3425 3244
Size: 181 5065
BsrGI T'GTAC_A
Cuts at: 810 1363 810
Size: 553 4693
BssSI C'ACGA_G
Cuts at: 3517 4225 3517
Size: 708 4538
BstAPI GCAn_nnn'nTGC
Cuts at: 2623 2623
Size: 5246
BstBI TT'CG_AA
Cuts at: 801 3607 801
Size: 2806 2440
BstXI CCAn_nnnn'nTGG
Cuts at: 775 920 775
Size: 145 5101
BstYI r'GATC_y
Cuts at: 1 150 957 1374 1624 2198 2271 2738 2761
Size: 149 807 417 250 574 73 467 23
Cuts at: 2761 3096 3342 3648 3660 3746 4798 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 807 574 467 449 417 335 306
250 246 149 86 73 23 12
BstZ17I GTA'TAC
Cuts at: 411 411
Size: 5246
Bsu36I CC'TnA_GG
Cuts at: 994 994
Size: 5246
BtgI C'CryG_G
Cuts at: 662 768 662
Size: 106 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4902
Fragments arranged by size:
4902 181 163
BtsI GCAGTG_nn'
Cuts at: 840 2489 840
Size: 1649 3597
DraI TTT'AAA
Cuts at: 2245 2245
Size: 5246
DrdI GACnn_nn'nnGTC
Cuts at: 2952 4296 2952
Size: 1344 3902
EaeI y'GGCC_r
Cuts at: 2831 3005 3396 3423 4768 2831
Size: 174 391 27 1345 3309
Fragments arranged by size:
3309 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5246
EarI CTCTTCn'nnn_
Cuts at: 305 685 2006 3269 3479 305
Size: 380 1321 1263 210 2072
Fragments arranged by size:
2072 1321 1263 380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 4196 4342 5056 3454
Size: 742 146 714 3644
Fragments arranged by size:
3644 742 714 146
EcoICRI GAG'CTC
Cuts at: 1918 2258 1918
Size: 340 4906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 1282 1363 1466 1925 1967 1982 2189 3071
Size: 796 81 103 459 42 15 207 882
Cuts at: 3071 3503 3856 4518 486
Size: 432 353 662 1214
Fragments arranged by size:
1214 882 796 662 459 432 353 207
103 81 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1481 1481
Size: 5246
EcoO109I rG'GnC_Cy
Cuts at: 2385 2385
Size: 5246
EcoRI G'AATT_C
Cuts at: 1309 1391 2250 1309
Size: 82 859 4305
Fragments arranged by size:
4305 859 82
EcoRV GAT'ATC
Cuts at: 1185 1320 1185
Size: 135 5111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1890 3265 1890
Size: 1375 3871
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5246
HaeII r_GCGC'y
Cuts at: 2680 2928 4158 4929 2680
Size: 248 1230 771 2997
Fragments arranged by size:
2997 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1782 1814 3088 3118 3120 3150 124
Size: 32 1626 32 1274 30 2 30 2220
Fragments arranged by size:
2220 1626 1274 32 32 30 30 2
HincII GTy'rAC
Cuts at: 676 717 918 2004 2285 676
Size: 41 201 1086 281 3637
Fragments arranged by size:
3637 1086 281 201 41
HindIII A'AGCT_T
Cuts at: 607 1937 607
Size: 1330 3916
HpaI GTT'AAC
Cuts at: 2004 2004
Size: 5246
KasI G'GCGC_C
Cuts at: 2676 2924 2676
Size: 248 4998
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5246
MluI A'CGCG_T
Cuts at: 4679 4679
Size: 5246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1714 4005 4189 1714
Size: 2291 184 2771
Fragments arranged by size:
2771 2291 184
MscI TGG'CCA
Cuts at: 3007 3007
Size: 5246
MslI CAynn'nnrTG
Cuts at: 1636 3235 3362 1636
Size: 1599 127 3520
Fragments arranged by size:
3520 1599 127
MspA1I CmG'CkG
Cuts at: 306 2357 2671 3031 3815 4060 306
Size: 2051 314 360 784 245 1492
Fragments arranged by size:
2051 1492 784 360 314 245
NaeI GCC'GGC
Cuts at: 3427 3427
Size: 5246
NarI GG'CG_CC
Cuts at: 2677 2925 2677
Size: 248 4998
NcoI C'CATG_G
Cuts at: 662 768 662
Size: 106 5140
NgoMIV G'CCGG_C
Cuts at: 3425 3425
Size: 5246
NspI r_CATG'y
Cuts at: 531 2299 2799 4402 531
Size: 1768 500 1603 1375
Fragments arranged by size:
1768 1603 1375 500
PasI CC'CwG_GG
Cuts at: 2211 2211
Size: 5246
PciI A'CATG_T
Cuts at: 4398 4398
Size: 5246
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 5214
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1081 1113 1081
Size: 32 5214
PstI C_TGCA'G
Cuts at: 965 1519 2293 2978 965
Size: 554 774 685 3233
Fragments arranged by size:
3233 774 685 554
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5246
PvuII CAG'CTG
Cuts at: 306 2671 3031 306
Size: 2365 360 2521
Fragments arranged by size:
2521 2365 360
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5246
SacI G_AGCT'C
Cuts at: 1920 2260 1920
Size: 340 4906
SalI G'TCGA_C
Cuts at: 674 2283 674
Size: 1609 3637
SapI GCTCTTCn'nnn_
Cuts at: 3269 3479 3269
Size: 210 5036
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5246
ScaI AGT'ACT
Cuts at: 4763 4763
Size: 5246
SfcI C'TryA_G
Cuts at: 33 853 961 1111 1515 1680 1740 2095 2289
Size: 820 108 150 404 165 60 355 194
Cuts at: 2289 2974 3942 4133 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 820 685 404 355 194 191
165 150 108 60
SfoI GGC'GCC
Cuts at: 2678 2926 2678
Size: 248 4998
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
SmaI CCC'GGG
Cuts at: 2268 2268
Size: 5246
SmlI C'TyrA_G
Cuts at: 1864 2401 3316 4030 4292 1864
Size: 537 915 714 262 2818
Fragments arranged by size:
2818 915 714 537 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5246
SphI G_CATG'C
Cuts at: 2299 2799 2299
Size: 500 4746
SspI AAT'ATT
Cuts at: 1245 1713 1245
Size: 468 4778
StyI C'CwwG_G
Cuts at: 662 768 2380 2734 662
Size: 106 1612 354 3174
Fragments arranged by size:
3174 1612 354 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 1385 2829 2962 3421 3630 675
Size: 710 1444 133 459 209 2291
Fragments arranged by size:
2291 1444 710 459 209 133
TatI w'GTAC_w
Cuts at: 810 1363 4761 810
Size: 553 3398 1295
Fragments arranged by size:
3398 1295 553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 5214
Tth111I GACn'n_nGTC
Cuts at: 3043 3043
Size: 5246
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1854 2741 1854
Size: 887 4359
XmaI C'CCGG_G
Cuts at: 2266 2266
Size: 5246
XmnI GAAnn'nnTTC
Cuts at: 908 908
Size: 5246
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BspEI BssHII BstEII ClaI
CspCI DraIII FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI