Restriction map for
pF1KA0726
(Circular) (Six-base) MAPSORT of: pF1KA0726.seq Check: 8344 from: 1 to: 5981
pF1KA0726 5981 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 283 1155 2134 283
Size: 872 979 4130
Fragments arranged by size:
4130 979 872
AccI GT'mk_AC
Cuts at: 3019 3019
Size: 5981
Acc65I G'GTAC_C
Cuts at: 2997 2997
Size: 5981
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1944 1999 2009 3806 4238 4591 5253 1944
Size: 55 10 1797 432 353 662 2672
Fragments arranged by size:
2672 1797 662 432 353 55 10
AflIII A'CryG_T
Cuts at: 3333 5133 5414 3333
Size: 1800 281 3900
Fragments arranged by size:
3900 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1656 1656
Size: 5981
AlwNI CAG_nnn'CTG
Cuts at: 883 1830 2037 2565 3286 4724 883
Size: 947 207 528 721 1438 2140
Fragments arranged by size:
2140 1438 947 721 528 207
ApaI G_GGCC'C
Cuts at: 460 1205 460
Size: 745 5236
ApaLI G'TGCA_C
Cuts at: 1108 2212 4819 1108
Size: 1104 2607 2270
Fragments arranged by size:
2607 2270 1104
ApoI r'AATT_y
Cuts at: 2234 2485 2985 5334 5590 2234
Size: 251 500 2349 256 2625
Fragments arranged by size:
2625 2349 500 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 5981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 1468 2263 3001 1468
Size: 795 738 4448
Fragments arranged by size:
4448 795 738
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 538 571 538
Size: 33 5948
BamHI G'GATC_C
Cuts at: 2259 3006 2259
Size: 747 5234
BanI G'GyrC_C
Cuts at: 330 1150 1167 1448 2997 3411 3659 3694 330
Size: 820 17 281 1549 414 248 35 2617
Fragments arranged by size:
2617 1549 820 414 281 248 35 17
BanII G_rGCy'C
Cuts at: 460 1198 1205 2321 2995 4025 460
Size: 738 7 1116 674 1030 2416
Fragments arranged by size:
2416 1116 1030 738 674 7
BbeI G_GCGC'C
Cuts at: 3415 3663 3415
Size: 248 5733
BbvCI CC'TCA_GC
Cuts at: 803 803
Size: 5981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1085 1119 1491 1525 1085
Size: 34 372 34 5541
Fragments arranged by size:
5541 372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 919 3879 4935 5981 919
Size: 2960 1056 1046 919
Fragments arranged by size:
2960 1056 1046 919
BclI T'GATC_A
Cuts at: 3501 3501
Size: 5981
BfrBI ATG'CAT
Cuts at: 1750 1750
Size: 5981
BglII A'GATC_T
Cuts at: 299 644 3496 5533 299
Size: 345 2852 2037 747
Fragments arranged by size:
2852 2037 747 345
BlpI GC'TnA_GC
Cuts at: 2548 3093 2548
Size: 545 5436
Bme1580I G_kGCm'C
Cuts at: 335 460 1112 1172 1205 1398 2179 2216 3606
Size: 125 652 60 33 193 781 37 1390
Cuts at: 3606 3699 4823 335
Size: 93 1124 1493
Fragments arranged by size:
1493 1390 1124 781 652 193 125 93
60 37 33
BmrI ACTGGGnnnn_n'
Cuts at: 529 627 1055 1178 1396 3193 3274 3609 5753
Size: 98 428 123 218 1797 81 335 2144
Cuts at: 5753 529
Size: 757
Fragments arranged by size:
2144 1797 757 428 335 218 123 98
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2440 4348 2411
Size: 29 1908 4044
Fragments arranged by size:
4044 1908 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1444 1738 1861 2335 2460 2741 2751 937
Size: 507 294 123 474 125 281 10 4167
Fragments arranged by size:
4167 507 474 294 281 125 123 10
Bpu10I CC'TnA_GC
Cuts at: 803 1707 803
Size: 904 5077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 3157 4036 4750 5048 1408
Size: 1749 879 714 298 2341
Fragments arranged by size:
2341 1749 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 913 1643 1852 2950 5685 913
Size: 730 209 1098 2735 1209
Fragments arranged by size:
2735 1209 1098 730 209
BsaAI yAC'GTr
Cuts at: 1812 2004 2544 3334 1812
Size: 192 540 790 4459
Fragments arranged by size:
4459 790 540 192
BsaBI GATnn'nnATC
Cuts at: 6 3495 5532 6
Size: 3489 2037 455
Fragments arranged by size:
3489 2037 455
BsaHI Gr'CG_yC
Cuts at: 1922 2024 2096 3412 3660 4362 5858 1922
Size: 102 72 1316 248 702 1496 2045
Fragments arranged by size:
2045 1496 1316 702 248 102 72
BsaWI w'CCGG_w
Cuts at: 3170 3691 4369 4780 4927 5201 3170
Size: 521 678 411 147 274 3950
Fragments arranged by size:
3950 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1489 1519 2614 2644 1489
Size: 30 1095 30 4826
Fragments arranged by size:
4826 1095 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1609 1609
Size: 5981
BseYI C'CCAG_C
Cuts at: 838 883 1191 2067 2350 2536 2555 2641 2929
Size: 45 308 876 283 186 19 86 288
Cuts at: 2929 3406 4164 4829 5237 838
Size: 477 758 665 408 1582
Fragments arranged by size:
1582 876 758 665 477 408 308 288
283 186 86 45 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2494 2551 1183
Size: 1311 57 4613
Fragments arranged by size:
4613 1311 57
BsiEI CG_ry'CG
Cuts at: 104 591 3523 3569 4799 104
Size: 487 2932 46 1230 1286
Fragments arranged by size:
2932 1286 1230 487 46
BsiHKAI G_wGCw'C
Cuts at: 1112 2216 2321 2549 2690 2995 3773 3963 4823
Size: 1104 105 228 141 305 778 190 860
Cuts at: 4823 1112
Size: 2270
Fragments arranged by size:
2270 1104 860 778 305 228 190 141
105
BsmBI CGTCTCn'nnnn_
Cuts at: 1983 2742 2937 1983
Size: 759 195 5027
Fragments arranged by size:
5027 759 195
Bsp1286I G_dGCh'C
Cuts at: 335 460 1112 1172 1198 1205 1398 2179 2216
Size: 125 652 60 26 7 193 781 37
Cuts at: 2216 2321 2549 2690 2995 3606 3699 3773 3963
Size: 105 228 141 305 611 93 74 190
Cuts at: 3963 4025 4823 335
Size: 62 798 1493
Fragments arranged by size:
1493 798 781 652 611 305 228 193
190 141 125 105 93 74 62 60
37 26 7
BspHI T'CATG_A
Cuts at: 1571 4413 1571
Size: 2842 3139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 283 1155 1419 2134 3031 3245 3547 3928 283
Size: 872 264 715 897 214 302 381 2336
Fragments arranged by size:
2336 897 872 715 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 1014 2268 4273 4327 5811 5976 50
Size: 964 1254 2005 54 1484 165 55
Fragments arranged by size:
2005 1484 1254 964 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2487 3893 2487
Size: 1406 4575
BsrFI r'CCGG_y
Cuts at: 2746 3979 4160 2746
Size: 1233 181 4567
Fragments arranged by size:
4567 1233 181
BssSI C'ACGA_G
Cuts at: 4252 4960 4252
Size: 708 5273
BstAPI GCAn_nnn'nTGC
Cuts at: 799 880 2565 3358 799
Size: 81 1685 793 3422
Fragments arranged by size:
3422 1685 793 81
BstBI TT'CG_AA
Cuts at: 4342 4342
Size: 5981
BstEII G'GTnAC_C
Cuts at: 2575 2575
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 325 978 1191 1534 325
Size: 653 213 343 4772
Fragments arranged by size:
4772 653 343 213
BstYI r'GATC_y
Cuts at: 1 299 644 1056 2033 2259 2381 3006 3473
Size: 298 345 412 977 226 122 625 467
Cuts at: 3473 3496 3831 4077 4383 4395 4481 5533 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 977 625 467 449 412 345 335
306 298 246 226 122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2386 2386
Size: 5981
BtgI C'CryG_G
Cuts at: 187 318 1350 1677 2571 2831 187
Size: 131 1032 327 894 260 3337
Fragments arranged by size:
3337 1032 894 327 260 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3931 4112 4275 3931
Size: 181 163 5637
Fragments arranged by size:
5637 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 2048 2910 3224 1943
Size: 105 862 314 4700
Fragments arranged by size:
4700 862 314 105
DraI TTT'AAA
Cuts at: 2980 2980
Size: 5981
DraIII CAC_nnn'GTG
Cuts at: 880 2145 880
Size: 1265 4716
DrdI GACnn_nn'nnGTC
Cuts at: 235 3687 5031 235
Size: 3452 1344 1185
Fragments arranged by size:
3452 1344 1185
EaeI y'GGCC_r
Cuts at: 980 2466 2918 3566 3740 4131 4158 5503 980
Size: 1486 452 648 174 391 27 1345 1458
Fragments arranged by size:
1486 1458 1345 648 452 391 174 27
EagI C'GGCC_G
Cuts at: 3566 3566
Size: 5981
EarI CTCTTCn'nnn_
Cuts at: 1277 1961 2372 2797 4004 4214 1277
Size: 684 411 425 1207 210 3044
Fragments arranged by size:
3044 1207 684 425 411 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 901 4189 4931 5077 5791 901
Size: 3288 742 146 714 1091
Fragments arranged by size:
3288 1091 742 714 146
EcoICRI GAG'CTC
Cuts at: 2319 2993 2319
Size: 674 5307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1444 1738 1861 1944 1999 2009 2335 2460
Size: 507 294 123 83 55 10 326 125
Cuts at: 2460 2741 2751 3806 4238 4591 5253 937
Size: 281 10 1055 432 353 662 1665
Fragments arranged by size:
1665 1055 662 507 432 353 326 294
281 125 123 83 55 10 10
EcoNI CCTnn'n_nnAGG
Cuts at: 355 2389 355
Size: 2034 3947
EcoO109I rG'GnC_Cy
Cuts at: 456 457 1202 1580 1928 2762 2781 3120 456
Size: 1 745 378 348 834 19 339 3317
Fragments arranged by size:
3317 834 745 378 348 339 19 1
EcoRI G'AATT_C
Cuts at: 2485 2985 2485
Size: 500 5481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5981
FspI TGC'GCA
Cuts at: 2713 3762 2713
Size: 1049 4932
FspAI rTGC'GCAy
Cuts at: 2713 2713
Size: 5981
HaeII r_GCGC'y
Cuts at: 1021 2648 3415 3663 4893 5664 1021
Size: 1627 767 248 1230 771 1338
Fragments arranged by size:
1627 1338 1230 771 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 991 1023 1312 1344 1489 1521 1642 1674 2278
Size: 32 289 32 145 32 121 32 604
Cuts at: 2278 2310 2936 2968 3823 3853 3855 3885 991
Size: 32 626 32 855 30 2 30 3087
Fragments arranged by size:
3087 855 626 604 289 145 121 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 513 3020 513
Size: 2507 3474
KasI G'GCGC_C
Cuts at: 3411 3659 3411
Size: 248 5733
KpnI G_GTAC'C
Cuts at: 3001 3001
Size: 5981
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2180 4740 4924 2180
Size: 2560 184 3237
Fragments arranged by size:
3237 2560 184
MscI TGG'CCA
Cuts at: 982 3742 982
Size: 2760 3221
MslI CAynn'nnrTG
Cuts at: 217 1532 2194 2368 2662 2665 2821 3970 4097
Size: 1315 662 174 294 3 156 1149 127
Cuts at: 4097 217
Size: 2101
Fragments arranged by size:
2101 1315 1149 662 294 174 156 127
3
MspA1I CmG'CkG
Cuts at: 282 842 2071 2307 3092 3406 3766 4550 4795
Size: 560 1229 236 785 314 360 784 245
Cuts at: 4795 282
Size: 1468
Fragments arranged by size:
1468 1229 785 784 560 360 314 245
236
NaeI GCC'GGC
Cuts at: 2748 4162 2748
Size: 1414 4567
NarI GG'CG_CC
Cuts at: 3412 3660 3412
Size: 248 5733
NcoI C'CATG_G
Cuts at: 187 318 1677 2831 187
Size: 131 1359 1154 3337
Fragments arranged by size:
3337 1359 1154 131
NgoMIV G'CCGG_C
Cuts at: 2746 4160 2746
Size: 1414 4567
NsiI A_TGCA'T
Cuts at: 1752 1752
Size: 5981
NspI r_CATG'y
Cuts at: 505 1754 1885 2199 3034 3534 5137 505
Size: 1249 131 314 835 500 1603 1349
Fragments arranged by size:
1603 1349 1249 835 500 314 131
PasI CC'CwG_GG
Cuts at: 1173 1173
Size: 5981
PciI A'CATG_T
Cuts at: 5133 5133
Size: 5981
PflMI CCAn_nnn'nTGG
Cuts at: 883 883
Size: 5981
PfoI T'CCnGG_A
Cuts at: 2282 2282
Size: 5981
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PmlI CAC'GTG
Cuts at: 1812 2544 1812
Size: 732 5249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 5949
PpuMI rG'GwC_Cy
Cuts at: 2781 2781
Size: 5981
PspOMI G'GGCC_C
Cuts at: 456 1201 456
Size: 745 5236
PspXI vC'TCGA_Gb
Cuts at: 1468 1468
Size: 5981
PstI C_TGCA'G
Cuts at: 728 968 1880 2303 3028 3713 728
Size: 240 912 423 725 685 2996
Fragments arranged by size:
2996 912 725 685 423 240
PvuI CG_AT'CG
Cuts at: 104 591 104
Size: 487 5494
PvuII CAG'CTG
Cuts at: 842 2071 3406 3766 842
Size: 1229 1335 360 3057
Fragments arranged by size:
3057 1335 1229 360
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5981
SacI G_AGCT'C
Cuts at: 2321 2995 2321
Size: 674 5307
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SapI GCTCTTCn'nnn_
Cuts at: 4004 4214 4004
Size: 210 5771
SbfI CC_TGCA'GG
Cuts at: 3028 3028
Size: 5981
ScaI AGT'ACT
Cuts at: 5498 5498
Size: 5981
SexAI A'CCwGG_T
Cuts at: 876 2141 876
Size: 1265 4716
SfcI C'TryA_G
Cuts at: 33 724 964 1113 1356 1770 1876 2299 3024
Size: 691 240 149 243 414 106 423 725
Cuts at: 3024 3709 4677 4868 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 725 691 685 423 414 243
240 191 149 106
SfoI GGC'GCC
Cuts at: 3413 3661 3413
Size: 248 5733
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SmaI CCC'GGG
Cuts at: 3003 3003
Size: 5981
SmlI C'TyrA_G
Cuts at: 1387 1468 3136 4051 4765 5027 1387
Size: 81 1668 915 714 262 2341
Fragments arranged by size:
2341 1668 915 714 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 3034 3534 3034
Size: 500 5481
StuI AGG'CCT
Cuts at: 439 439
Size: 5981
StyI C'CwwG_G
Cuts at: 187 263 318 386 983 1644 1677 2777 2831
Size: 76 55 68 597 661 33 1100 54
Cuts at: 2831 3115 3469 187
Size: 284 354 2699
Fragments arranged by size:
2699 1100 661 597 354 284 76 68
55 54 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 684 1165 1168 1562 1657 3564 3697 4156 4365
Size: 481 3 394 95 1907 133 459 209
Cuts at: 4365 684
Size: 2300
Fragments arranged by size:
2300 1907 481 459 394 209 133 95
3
TatI w'GTAC_w
Cuts at: 635 728 1630 5496 635
Size: 93 902 3866 1120
Fragments arranged by size:
3866 1120 902 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1017 1049 1091 1123 2614 2646 5498 5530 1017
Size: 32 42 32 1491 32 2852 32 1468
Fragments arranged by size:
2852 1491 1468 42 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3778 3778
Size: 5981
XbaI T'CTAG_A
Cuts at: 3012 3012
Size: 5981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 1050 1675 1989 2792 3476 393
Size: 657 625 314 803 684 2898
Fragments arranged by size:
2898 803 684 657 625 314
XhoI C'TCGA_G
Cuts at: 1468 1468
Size: 5981
XmaI C'CCGG_G
Cuts at: 3001 3001
Size: 5981
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BbsI BglI BmgBI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI