Restriction map for
pF1KA0732
(Circular) (Six-base) MAPSORT of: pF1KA0732.seq Check: 5324 from: 1 to: 7370
pF1KA0732 7370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3076 3076
Size: 7370
AccI GT'mk_AC
Cuts at: 2440 2629 3283 4408 2440
Size: 189 654 1125 5402
Fragments arranged by size:
5402 1125 654 189
Acc65I G'GTAC_C
Cuts at: 2318 2369 4386 2318
Size: 51 2017 5302
Fragments arranged by size:
5302 2017 51
AclI AA'CG_TT
Cuts at: 7210 7210
Size: 7370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 2158 2395 3184 3520 3707 5195 5627 6374
Size: 1155 237 789 336 187 1488 432 747
Cuts at: 6374 6642 1003
Size: 268 1731
Fragments arranged by size:
1731 1488 1155 789 747 432 336 268
237 187
AfeI AGC'GCT
Cuts at: 835 909 1981 835
Size: 74 1072 6224
Fragments arranged by size:
6224 1072 74
AflII C'TTAA_G
Cuts at: 3076 3076
Size: 7370
AflIII A'CryG_T
Cuts at: 4722 5826 6803 4722
Size: 1104 977 5289
Fragments arranged by size:
5289 1104 977
AgeI A'CCGG_T
Cuts at: 5758 5758
Size: 7370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3495 3495
Size: 7370
AlwNI CAG_nnn'CTG
Cuts at: 1654 1848 1884 2034 2181 2854 3685 4675 6242
Size: 194 36 150 147 673 831 990 1567
Cuts at: 6242 1654
Size: 2782
Fragments arranged by size:
2782 1567 990 831 673 194 150 147
36
ApaI G_GGCC'C
Cuts at: 1708 4356 1708
Size: 2648 4722
ApaLI G'TGCA_C
Cuts at: 6140 6140
Size: 7370
ApoI r'AATT_y
Cuts at: 325 2587 4374 6723 6979 325
Size: 2262 1787 2349 256 716
Fragments arranged by size:
2349 2262 1787 716 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7370
AvaI C'yCGr_G
Cuts at: 1804 3399 4048 4390 1804
Size: 1595 649 342 4784
Fragments arranged by size:
4784 1595 649 342
AvrII C'CTAG_G
Cuts at: 472 472
Size: 7370
BamHI G'GATC_C
Cuts at: 158 2088 4395 158
Size: 1930 2307 3133
Fragments arranged by size:
3133 2307 1930
BanI G'GyrC_C
Cuts at: 1456 2318 2369 2405 3333 3576 3930 4386 4800
Size: 862 51 36 928 243 354 456 414
Cuts at: 4800 5048 5083 1456
Size: 248 35 3743
Fragments arranged by size:
3743 928 862 456 414 354 248 243
51 36 35
BanII G_rGCy'C
Cuts at: 718 826 1708 2138 2602 3830 4089 4356 4384
Size: 108 882 430 464 1228 259 267 28
Cuts at: 4384 5414 718
Size: 1030 2674
Fragments arranged by size:
2674 1228 1030 882 464 430 267 259
108 28
BbeI G_GCGC'C
Cuts at: 2409 4804 5052 2409
Size: 2395 248 4727
Fragments arranged by size:
4727 2395 248
BbsI GAAGACnn'nnnn_
Cuts at: 2216 2690 3137 3668 2216
Size: 474 447 531 5918
Fragments arranged by size:
5918 531 474 447
BbvCI CC'TCA_GC
Cuts at: 911 911
Size: 7370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3751 3785 4020 4054 4092 4126 3751
Size: 34 235 34 38 34 6995
Fragments arranged by size:
6995 235 38 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1676 5268 6029 7370 1676
Size: 3592 761 1341 1676
Fragments arranged by size:
3592 1676 1341 761
BclI T'GATC_A
Cuts at: 1491 2273 3944 4890 1491
Size: 782 1671 946 3971
Fragments arranged by size:
3971 1671 946 782
BfrBI ATG'CAT
Cuts at: 2291 2291
Size: 7370
BglI GCCn_nnn'nGGC
Cuts at: 181 1454 3892 4248 181
Size: 1273 2438 356 3303
Fragments arranged by size:
3303 2438 1273 356
BglII A'GATC_T
Cuts at: 249 539 2669 4885 6922 249
Size: 290 2130 2216 2037 697
Fragments arranged by size:
2216 2130 2037 697 290
BlpI GC'TnA_GC
Cuts at: 888 1891 3095 3758 4482 888
Size: 1003 1204 663 724 3776
Fragments arranged by size:
3776 1204 1003 724 663
Bme1580I G_kGCm'C
Cuts at: 1708 2283 2710 3935 4356 4995 5088 6144 1708
Size: 575 427 1225 421 639 93 1056 2934
Fragments arranged by size:
2934 1225 1056 639 575 427 421 93
BmrI ACTGGGnnnn_n'
Cuts at: 1656 1693 2756 3173 4582 4663 4998 7142 1656
Size: 37 1063 417 1409 81 335 2144 1884
Fragments arranged by size:
2144 1884 1409 1063 417 335 81 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3015 3471 5737 3015
Size: 456 2266 4648
Fragments arranged by size:
4648 2266 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1364 1890 1954 2686 3817 3925 142
Size: 1222 526 64 732 1131 108 3587
Fragments arranged by size:
3587 1222 1131 732 526 108 64
Bpu10I CC'TnA_GC
Cuts at: 283 911 1589 2596 4211 283
Size: 628 678 1007 1615 3442
Fragments arranged by size:
3442 1615 1007 678 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1272 2851 4546 5425 5917 6215 1272
Size: 1579 1695 879 492 298 2427
Fragments arranged by size:
2427 1695 1579 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2536 7074 2536
Size: 4538 2832
BsaAI yAC'GTr
Cuts at: 3444 4349 4723 3444
Size: 905 374 6091
Fragments arranged by size:
6091 905 374
BsaBI GATnn'nnATC
Cuts at: 6 4884 6921 6
Size: 4878 2037 455
Fragments arranged by size:
4878 2037 455
BsaHI Gr'CG_yC
Cuts at: 243 1519 2406 4801 5049 5751 7247 243
Size: 1276 887 2395 248 702 1496 366
Fragments arranged by size:
2395 1496 1276 887 702 366 248
BsaWI w'CCGG_w
Cuts at: 1923 4283 4559 5080 5758 6032 6179 6590 1923
Size: 2360 276 521 678 274 147 411 2703
Fragments arranged by size:
2703 2360 678 521 411 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1888 1918 1888
Size: 30 7340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 1822 2460 3004 3224 3655 3658 4252 613
Size: 1209 638 544 220 431 3 594 3731
Fragments arranged by size:
3731 1209 638 594 544 431 220 3
BseYI C'CCAG_C
Cuts at: 1523 2282 2381 2745 2966 3994 4152 4335 4795
Size: 759 99 364 221 1028 158 183 460
Cuts at: 4795 5553 6130 6626 1523
Size: 758 577 496 2267
Fragments arranged by size:
2267 1028 759 758 577 496 460 364
221 183 158 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1834 3048 1834
Size: 1214 6156
BsiEI CG_ry'CG
Cuts at: 104 4912 4958 6166 104
Size: 4808 46 1208 1308
Fragments arranged by size:
4808 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2138 3830 4384 5162 5352 6144 2138
Size: 1692 554 778 190 792 3364
Fragments arranged by size:
3364 1692 792 778 554 190
BsmI GAATG_Cn'
Cuts at: 1328 2049 3547 1328
Size: 721 1498 5151
Fragments arranged by size:
5151 1498 721
Bsp1286I G_dGCh'C
Cuts at: 718 826 1708 2138 2283 2602 2710 3830 3935
Size: 108 882 430 145 319 108 1120 105
Cuts at: 3935 4089 4356 4384 4995 5088 5162 5352 5414
Size: 154 267 28 611 93 74 190 62
Cuts at: 5414 6144 718
Size: 730 1944
Fragments arranged by size:
1944 1120 882 730 611 430 319 267
190 154 145 108 108 105 93 74
62 28
BspEI T'CCGG_A
Cuts at: 1923 6590 1923
Size: 4667 2703
BspHI T'CATG_A
Cuts at: 2525 6546 2525
Size: 4021 3349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 542 1363 1509 1841 1907 2175 3076 4298 4420
Size: 821 146 332 66 268 901 1222 122
Cuts at: 4420 4634 4936 5317 542
Size: 214 302 381 2595
Fragments arranged by size:
2595 1222 901 821 381 332 302 268
214 146 122 66
BsrBI CCG'CTC
Cuts at: 50 845 2999 5662 5716 7200 7365 50
Size: 795 2154 2663 54 1484 165 55
Fragments arranged by size:
2663 2154 1484 795 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 4110 5282 2387
Size: 1723 1172 4475
Fragments arranged by size:
4475 1723 1172
BsrFI r'CCGG_y
Cuts at: 5368 5549 5758 5368
Size: 181 209 6980
Fragments arranged by size:
6980 209 181
BsrGI T'GTAC_A
Cuts at: 3341 3341
Size: 7370
BssSI C'ACGA_G
Cuts at: 5641 5999 5641
Size: 358 7012
BstAPI GCAn_nnn'nTGC
Cuts at: 2297 4747 2297
Size: 2450 4920
BstBI TT'CG_AA
Cuts at: 5731 5731
Size: 7370
BstXI CCAn_nnnn'nTGG
Cuts at: 594 2664 2806 3246 3735 3994 594
Size: 2070 142 440 489 259 3970
Fragments arranged by size:
3970 2070 489 440 259 142
BstYI r'GATC_y
Cuts at: 1 134 158 249 539 1417 1432 2030 2088
Size: 133 24 91 290 878 15 598 58
Cuts at: 2088 2669 3249 3782 4395 4862 4885 5220 5466
Size: 581 580 533 613 467 23 335 246
Cuts at: 5466 6478 6564 6576 6922 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 878 613 598 581 580 533 467
449 346 335 290 246 133 91 86
58 24 23 15 12
BstZ17I GTA'TAC
Cuts at: 2441 2441
Size: 7370
Bsu36I CC'TnA_GG
Cuts at: 3110 3428 3110
Size: 318 7052
BtgI C'CryG_G
Cuts at: 107 810 1107 1199 1449 2692 2805 3593 4098
Size: 703 297 92 250 1243 113 788 505
Cuts at: 4098 107
Size: 3379
Fragments arranged by size:
3379 1243 788 703 505 297 250 113
92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2301 4104 5320 5501 5664 2301
Size: 1803 1216 181 163 4007
Fragments arranged by size:
4007 1803 1216 181 163
BtsI GCAGTG_nn'
Cuts at: 1263 2441 2855 3323 4137 4613 1263
Size: 1178 414 468 814 476 4020
Fragments arranged by size:
4020 1178 814 476 468 414
DraI TTT'AAA
Cuts at: 4369 4369
Size: 7370
DrdI GACnn_nn'nnGTC
Cuts at: 5076 5934 5076
Size: 858 6512
EaeI y'GGCC_r
Cuts at: 1104 3035 3743 3884 3965 4146 4955 5129 5520
Size: 1931 708 141 81 181 809 174 391
Cuts at: 5520 5547 6892 1104
Size: 27 1345 1582
Fragments arranged by size:
1931 1582 1345 809 708 391 181 174
141 81 27
EagI C'GGCC_G
Cuts at: 4955 4955
Size: 7370
EarI CTCTTCn'nnn_
Cuts at: 1072 1160 2534 3377 3527 5393 5603 1072
Size: 88 1374 843 150 1866 210 2839
Fragments arranged by size:
2839 1866 1374 843 210 150 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 573 2628 3046 3075 5578 5888 6034 7180
Size: 446 2055 418 29 2503 310 146 1146
Cuts at: 7180 127
Size: 317
Fragments arranged by size:
2503 2055 1146 446 418 317 310 146
29
EcoICRI GAG'CTC
Cuts at: 2136 3828 4382 2136
Size: 1692 554 5124
Fragments arranged by size:
5124 1692 554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1003 1364 1890 1954 2158 2395 2686 3184
Size: 861 361 526 64 204 237 291 498
Cuts at: 3184 3520 3707 3817 3925 5195 5627 6374 6642
Size: 336 187 110 108 1270 432 747 268
Cuts at: 6642 142
Size: 870
Fragments arranged by size:
1270 870 861 747 526 498 432 361
336 291 268 237 204 187 110 108
64
EcoNI CCTnn'n_nnAGG
Cuts at: 471 616 1244 3382 3967 471
Size: 145 628 2138 585 3874
Fragments arranged by size:
3874 2138 628 585 145
EcoO109I rG'GnC_Cy
Cuts at: 1054 1399 1476 1651 1705 1729 1929 2862 3348
Size: 345 77 175 54 24 200 933 486
Cuts at: 3348 3432 4509 1054
Size: 84 1077 3915
Fragments arranged by size:
3915 1077 933 486 345 200 175 84
77 54 24
EcoRI G'AATT_C
Cuts at: 325 4374 325
Size: 4049 3321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5389 5389
Size: 7370
FspI TGC'GCA
Cuts at: 2479 5151 2479
Size: 2672 4698
HaeII r_GCGC'y
Cuts at: 837 911 1983 2409 4306 4804 5052 6074 7053
Size: 74 1072 426 1897 498 248 1022 979
Cuts at: 7053 837
Size: 1154
Fragments arranged by size:
1897 1154 1072 1022 979 498 426 248
74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2968 3000 3241 3265 3273 3297 5212 5242 5244
Size: 32 241 24 8 24 1915 30 2
Cuts at: 5244 5274 2968
Size: 30 5064
Fragments arranged by size:
5064 1915 241 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 3284 4274 4409 3284
Size: 990 135 6245
Fragments arranged by size:
6245 990 135
HindIII A'AGCT_T
Cuts at: 2452 2452
Size: 7370
KasI G'GCGC_C
Cuts at: 2405 4800 5048 2405
Size: 2395 248 4727
Fragments arranged by size:
4727 2395 248
KpnI G_GTAC'C
Cuts at: 2322 2373 4390 2322
Size: 51 2017 5302
Fragments arranged by size:
5302 2017 51
MluI A'CGCG_T
Cuts at: 6803 6803
Size: 7370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 2625 6041 6225 125
Size: 2500 3416 184 1270
Fragments arranged by size:
3416 2500 1270 184
MscI TGG'CCA
Cuts at: 3037 3745 3886 3967 4148 5131 3037
Size: 708 141 81 181 983 5276
Fragments arranged by size:
5276 983 708 181 141 81
MslI CAynn'nnrTG
Cuts at: 2804 2944 3139 3925 5359 5486 2804
Size: 140 195 786 1434 127 4688
Fragments arranged by size:
4688 1434 786 195 140 127
MspA1I CmG'CkG
Cuts at: 812 892 2970 3062 3099 3475 3695 4152 4481
Size: 80 2078 92 37 376 220 457 329
Cuts at: 4481 4795 5155 6168 6413 812
Size: 314 360 1013 245 1769
Fragments arranged by size:
2078 1769 1013 457 376 360 329 314
245 220 92 80 37
NaeI GCC'GGC
Cuts at: 5551 5551
Size: 7370
NarI GG'CG_CC
Cuts at: 2406 4801 5049 2406
Size: 2395 248 4727
Fragments arranged by size:
4727 2395 248
NcoI C'CATG_G
Cuts at: 107 1199 2805 3593 107
Size: 1092 1606 788 3884
Fragments arranged by size:
3884 1606 1092 788
NgoMIV G'CCGG_C
Cuts at: 5549 5549
Size: 7370
NsiI A_TGCA'T
Cuts at: 2293 2293
Size: 7370
NspI r_CATG'y
Cuts at: 2644 2925 3265 4423 4923 5830 2644
Size: 281 340 1158 500 907 4184
Fragments arranged by size:
4184 1158 907 500 340 281
PasI CC'CwG_GG
Cuts at: 313 1726 2703 313
Size: 1413 977 4980
Fragments arranged by size:
4980 1413 977
PciI A'CATG_T
Cuts at: 5826 5826
Size: 7370
PflMI CCAn_nnn'nTGG
Cuts at: 724 724
Size: 7370
PfoI T'CCnGG_A
Cuts at: 3008 3155 3377 3794 3008
Size: 147 222 417 6584
Fragments arranged by size:
6584 417 222 147
PmeI GTTT'AAAC
Cuts at: 4369 4369
Size: 7370
PmlI CAC'GTG
Cuts at: 4349 4349
Size: 7370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1874 1906 6509 6541 1874
Size: 32 4603 32 2703
Fragments arranged by size:
4603 2703 32 32
PpuMI rG'GwC_Cy
Cuts at: 1054 1399 1476 2862 3348 3432 1054
Size: 345 77 1386 486 84 4992
Fragments arranged by size:
4992 1386 486 345 84 77
PshAI GACnn'nnGTC
Cuts at: 3806 3806
Size: 7370
PspOMI G'GGCC_C
Cuts at: 1704 4352 1704
Size: 2648 4722
PspXI vC'TCGA_Gb
Cuts at: 4048 4048
Size: 7370
PstI C_TGCA'G
Cuts at: 539 1838 1889 1970 2186 2850 2891 2930 3318
Size: 1299 51 81 216 664 41 39 388
Cuts at: 3318 4417 5102 539
Size: 1099 685 2807
Fragments arranged by size:
2807 1299 1099 685 664 388 216 81
51 41 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7370
PvuII CAG'CTG
Cuts at: 892 2970 3099 3475 4152 4795 5155 892
Size: 2078 129 376 677 643 360 3107
Fragments arranged by size:
3107 2078 677 643 376 360 129
RsrII CG'GwC_CG
Cuts at: 5565 5565
Size: 7370
SacI G_AGCT'C
Cuts at: 2138 3830 4384 2138
Size: 1692 554 5124
Fragments arranged by size:
5124 1692 554
SacII CC_GC'GG
Cuts at: 813 813
Size: 7370
SalI G'TCGA_C
Cuts at: 3282 4407 3282
Size: 1125 6245
SanDI GG'GwC_CC
Cuts at: 1476 1476
Size: 7370
SapI GCTCTTCn'nnn_
Cuts at: 3527 5393 5603 3527
Size: 1866 210 5294
Fragments arranged by size:
5294 1866 210
SbfI CC_TGCA'GG
Cuts at: 4417 4417
Size: 7370
ScaI AGT'ACT
Cuts at: 6887 6887
Size: 7370
SexAI A'CCwGG_T
Cuts at: 617 617
Size: 7370
SfcI C'TryA_G
Cuts at: 33 434 535 1690 1834 1885 1966 2182 2846
Size: 401 101 1155 144 51 81 216 664
Cuts at: 2846 2887 2926 3314 4413 5098 6091 6282 33
Size: 41 39 388 1099 685 993 191 1121
Fragments arranged by size:
1155 1121 1099 993 685 664 401 388
216 191 144 101 81 51 41 39
SfiI GGCCn_nnn'nGGCC
Cuts at: 181 181
Size: 7370
SfoI GGC'GCC
Cuts at: 2407 4802 5050 2407
Size: 2395 248 4727
Fragments arranged by size:
4727 2395 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7370
SmaI CCC'GGG
Cuts at: 4392 4392
Size: 7370
SmlI C'TyrA_G
Cuts at: 1251 2866 3076 4048 4525 5440 5932 6194 1251
Size: 1615 210 972 477 915 492 262 2427
Fragments arranged by size:
2427 1615 972 915 492 477 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7370
SphI G_CATG'C
Cuts at: 2925 4423 4923 2925
Size: 1498 500 5372
Fragments arranged by size:
5372 1498 500
StyI C'CwwG_G
Cuts at: 107 472 965 1199 2301 2513 2805 3593 3968
Size: 365 493 234 1102 212 292 788 375
Cuts at: 3968 4504 4858 107
Size: 536 354 2619
Fragments arranged by size:
2619 1102 788 536 493 375 365 354
292 234 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 365 4953 5086 5545 5754 365
Size: 4588 133 459 209 1981
Fragments arranged by size:
4588 1981 459 209 133
TatI w'GTAC_w
Cuts at: 2955 3341 3917 6885 2955
Size: 386 576 2968 3440
Fragments arranged by size:
3440 2968 576 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 634 666 1754 1785 1786 1817 3213 3245 3551
Size: 32 1088 31 1 31 1396 32 306
Cuts at: 3551 3583 4278 4310 6887 6919 634
Size: 32 695 32 2577 32 1085
Fragments arranged by size:
2577 1396 1088 1085 695 306 32 32
32 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1755 2461 5167 1755
Size: 706 2706 3958
Fragments arranged by size:
3958 2706 706
XbaI T'CTAG_A
Cuts at: 4401 4401
Size: 7370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 424 1221 4865 424
Size: 797 3644 2929
Fragments arranged by size:
3644 2929 797
XhoI C'TCGA_G
Cuts at: 4048 4048
Size: 7370
XmaI C'CCGG_G
Cuts at: 4390 4390
Size: 7370
XmnI GAAnn'nnTTC
Cuts at: 467 2047 2114 467
Size: 1580 67 5723
Fragments arranged by size:
5723 1580 67
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AloI AscI BaeI BmgBI BmtI
BsiWI BsmBI BssHII BstEII ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PsiI PsrI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI