Restriction map for
pF1KA0734
(Circular) (Six-base) MAPSORT of: pF1KA0734.seq Check: 3460 from: 1 to: 6674
pF1KA0734 6674 bp
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 243 249 609 1334 243
Size: 6 360 725 5583
Fragments arranged by size:
5583 725 360 6
AatII G_ACGT'C
Cuts at: 1988 2924 1988
Size: 936 5738
AccI GT'mk_AC
Cuts at: 217 1190 3341 3712 217
Size: 973 2151 371 3179
Fragments arranged by size:
3179 2151 973 371
Acc65I G'GTAC_C
Cuts at: 562 3690 562
Size: 3128 3546
AclI AA'CG_TT
Cuts at: 6514 6514
Size: 6674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 735 1072 1741 1831 1915 2236 3061 4499
Size: 323 337 669 90 84 321 825 1438
Cuts at: 4499 4931 5284 5946 412
Size: 432 353 662 1140
Fragments arranged by size:
1438 1140 825 669 662 432 353 337
323 321 90 84
AfeI AGC'GCT
Cuts at: 829 2128 829
Size: 1299 5375
AflII C'TTAA_G
Cuts at: 3240 3240
Size: 6674
AflIII A'CryG_T
Cuts at: 2423 4026 5826 6107 2423
Size: 1603 1800 281 2990
Fragments arranged by size:
2990 1800 1603 281
AgeI A'CCGG_T
Cuts at: 3134 3134
Size: 6674
AhdI GACnn_n'nnGTC
Cuts at: 3432 3432
Size: 6674
AleI CACnn'nnGTG
Cuts at: 895 895
Size: 6674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 344 344
Size: 6674
AlwNI CAG_nnn'CTG
Cuts at: 1388 1653 1887 2323 2400 2649 2904 3186 3979
Size: 265 234 436 77 249 255 282 793
Cuts at: 3979 5417 1388
Size: 1438 2645
Fragments arranged by size:
2645 1438 793 436 282 265 255 249
234 77
ApaI G_GGCC'C
Cuts at: 142 574 2606 3274 142
Size: 432 2032 668 3542
Fragments arranged by size:
3542 2032 668 432
ApaLI G'TGCA_C
Cuts at: 812 5512 812
Size: 4700 1974
ApoI r'AATT_y
Cuts at: 3678 6027 6283 3678
Size: 2349 256 4069
Fragments arranged by size:
4069 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
AvaI C'yCGr_G
Cuts at: 116 349 427 1862 2085 2605 3694 116
Size: 233 78 1435 223 520 1089 3096
Fragments arranged by size:
3096 1435 1089 520 233 223 78
AvrII C'CTAG_G
Cuts at: 3471 3471
Size: 6674
BamHI G'GATC_C
Cuts at: 474 1841 3699 474
Size: 1367 1858 3449
Fragments arranged by size:
3449 1858 1367
BanI G'GyrC_C
Cuts at: 163 562 628 1148 1173 2404 3690 4104 4352
Size: 399 66 520 25 1231 1286 414 248
Cuts at: 4352 4387 163
Size: 35 2450
Fragments arranged by size:
2450 1286 1231 520 414 399 248 66
35 25
BanII G_rGCy'C
Cuts at: 142 498 574 809 1376 1403 1443 2062 2093
Size: 356 76 235 567 27 40 619 31
Cuts at: 2093 2102 2285 2543 2606 3274 3564 3688 4718
Size: 9 183 258 63 668 290 124 1030
Cuts at: 4718 142
Size: 2098
Fragments arranged by size:
2098 1030 668 619 567 356 290 258
235 183 124 76 63 40 31 27
9
BbeI G_GCGC'C
Cuts at: 4108 4356 4108
Size: 248 6426
BbsI GAAGACnn'nnnn_
Cuts at: 1980 3325 1980
Size: 1345 5329
BbvCI CC'TCA_GC
Cuts at: 2027 2227 2338 3580 2027
Size: 200 111 1242 5121
Fragments arranged by size:
5121 1242 200 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 153 187 153
Size: 34 6640
BciVI GTATCCnnnnn_n'
Cuts at: 1017 1275 4572 5628 6674 1017
Size: 258 3297 1056 1046 1017
Fragments arranged by size:
3297 1056 1046 1017 258
BclI T'GATC_A
Cuts at: 1265 4194 1265
Size: 2929 3745
BglI GCCn_nnn'nGGC
Cuts at: 337 1055 3476 337
Size: 718 2421 3535
Fragments arranged by size:
3535 2421 718
BglII A'GATC_T
Cuts at: 1763 3571 4189 6226 1763
Size: 1808 618 2037 2211
Fragments arranged by size:
2211 2037 1808 618
BlpI GC'TnA_GC
Cuts at: 604 1336 1402 2056 2632 3176 3786 604
Size: 732 66 654 576 544 610 3492
Fragments arranged by size:
3492 732 654 610 576 544 66
Bme1580I G_kGCm'C
Cuts at: 142 166 268 574 816 2407 2606 3274 4299
Size: 24 102 306 242 1591 199 668 1025
Cuts at: 4299 4392 5516 142
Size: 93 1124 1300
Fragments arranged by size:
1591 1300 1124 1025 668 306 242 199
102 93 24
BmgBI CAC'GTC
Cuts at: 2424 2424
Size: 6674
BmrI ACTGGGnnnn_n'
Cuts at: 1575 3886 3967 4302 6446 1575
Size: 2311 81 335 2144 1803
Fragments arranged by size:
2311 2144 1803 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1233 1395 1417 2100 5041 1233
Size: 162 22 683 2941 2866
Fragments arranged by size:
2941 2866 683 162 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 598 1203 1228 1365 1567 1729 2032 2095
Size: 385 605 25 137 202 162 303 63
Cuts at: 2095 2107 2283 2299 2869 2881 2980 3028 3145
Size: 12 176 16 570 12 99 48 117
Cuts at: 3145 3664 213
Size: 519 3223
Fragments arranged by size:
3223 605 570 519 385 303 202 176
162 137 117 99 63 48 25 16
12 12
Bpu10I CC'TnA_GC
Cuts at: 247 2027 2227 2338 2393 2848 3148 3580 247
Size: 1780 200 111 55 455 300 432 3341
Fragments arranged by size:
3341 1780 455 432 300 200 111 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1243 3619 3850 4729 5443 5741 658
Size: 585 2376 231 879 714 298 1591
Fragments arranged by size:
2376 1591 879 714 585 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 105 2335 6378 105
Size: 2230 4043 401
Fragments arranged by size:
4043 2230 401
BsaAI yAC'GTr
Cuts at: 689 4027 689
Size: 3338 3336
BsaBI GATnn'nnATC
Cuts at: 6 4188 6225 6
Size: 4182 2037 455
Fragments arranged by size:
4182 2037 455
BsaHI Gr'CG_yC
Cuts at: 794 1187 1913 1985 2291 2921 3144 3230 3479
Size: 393 726 72 306 630 223 86 249
Cuts at: 3479 4105 4353 5055 6551 794
Size: 626 248 702 1496 917
Fragments arranged by size:
1496 917 726 702 630 626 393 306
249 248 223 86 72
BsaWI w'CCGG_w
Cuts at: 3134 3863 4384 5062 5473 5620 5894 3134
Size: 729 521 678 411 147 274 3914
Fragments arranged by size:
3914 729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 2281 2296 2311 2326 2854 2884 523
Size: 30 1728 15 15 15 528 30 4313
Fragments arranged by size:
4313 1728 528 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 523 544 607 655 1384 1409 1615 2044
Size: 313 21 63 48 729 25 206 429
Cuts at: 2044 2065 2286 2872 3061 3597 210
Size: 21 221 586 189 536 3287
Fragments arranged by size:
3287 729 586 536 429 313 221 206
189 63 48 25 21 21
BseYI C'CCAG_C
Cuts at: 478 771 1536 2400 2408 2557 2904 3267 3451
Size: 293 765 864 8 149 347 363 184
Cuts at: 3451 3523 4099 4857 5522 5930 478
Size: 72 576 758 665 408 1222
Fragments arranged by size:
1222 864 765 758 665 576 408 363
347 293 184 149 72 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 277 958 1044 1255 1417 1462 1497 2622
Size: 138 681 86 211 162 45 35 1125
Cuts at: 2622 3043 3187 3316 3526 139
Size: 421 144 129 210 3287
Fragments arranged by size:
3287 1125 681 421 211 210 162 144
138 129 86 45 35
BsiEI CG_ry'CG
Cuts at: 104 1194 2492 4216 4262 5492 104
Size: 1090 1298 1724 46 1230 1286
Fragments arranged by size:
1724 1298 1286 1230 1090 46
BsiHKAI G_wGCw'C
Cuts at: 248 647 816 935 964 1361 1403 2102 2543
Size: 399 169 119 29 397 42 699 441
Cuts at: 2543 2618 3688 4466 4656 5516 248
Size: 75 1070 778 190 860 1406
Fragments arranged by size:
1406 1070 860 778 699 441 399 397
190 169 119 75 42 29
BsmBI CGTCTCn'nnnn_
Cuts at: 2928 2928
Size: 6674
Bsp1286I G_dGCh'C
Cuts at: 142 166 248 268 498 574 647 809 816
Size: 24 82 20 230 76 73 162 7
Cuts at: 816 935 964 1361 1376 1403 1443 2062 2093
Size: 119 29 397 15 27 40 619 31
Cuts at: 2093 2102 2285 2407 2543 2606 2618 3274 3564
Size: 9 183 122 136 63 12 656 290
Cuts at: 3564 3688 4299 4392 4466 4656 4718 5516 142
Size: 124 611 93 74 190 62 798 1300
Fragments arranged by size:
1300 798 656 619 611 397 290 230
190 183 162 136 124 122 119 93
82 76 74 73 63 62 40 31
29 27 24 20 15 12 9 7
BspHI T'CATG_A
Cuts at: 993 2789 5106 993
Size: 1796 2317 2561
Fragments arranged by size:
2561 2317 1796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 243 249 609 1055 1334 1476 1519 1541
Size: 82 6 360 446 279 142 43 22
Cuts at: 1541 3224 3724 3938 4240 4621 161
Size: 1683 500 214 302 381 2214
Fragments arranged by size:
2214 1683 500 446 381 360 302 279
214 142 82 43 22 6
BsrBI CCG'CTC
Cuts at: 50 2162 2787 4966 5020 6504 6669 50
Size: 2112 625 2179 54 1484 165 55
Fragments arranged by size:
2179 2112 1484 625 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 4586 1796
Size: 2790 3884
BsrFI r'CCGG_y
Cuts at: 2491 3134 3551 4672 4853 2491
Size: 643 417 1121 181 4312
Fragments arranged by size:
4312 1121 643 417 181
BsrGI T'GTAC_A
Cuts at: 539 2730 539
Size: 2191 4483
BssSI C'ACGA_G
Cuts at: 2542 4945 5653 2542
Size: 2403 708 3563
Fragments arranged by size:
3563 2403 708
BstAPI GCAn_nnn'nTGC
Cuts at: 130 1653 2323 4051 130
Size: 1523 670 1728 2753
Fragments arranged by size:
2753 1728 1523 670
BstBI TT'CG_AA
Cuts at: 5035 5035
Size: 6674
BstEII G'GTnAC_C
Cuts at: 148 203 888 148
Size: 55 685 5934
Fragments arranged by size:
5934 685 55
BstYI r'GATC_y
Cuts at: 1 474 1763 1841 3571 3699 4166 4189 4524
Size: 473 1289 78 1730 128 467 23 335
Cuts at: 4524 4770 5076 5088 5174 6226 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1730 1289 1052 473 467 449 335 306
246 128 86 78 23 12
BstZ17I GTA'TAC
Cuts at: 3342 3342
Size: 6674
Bsu36I CC'TnA_GG
Cuts at: 3016 3016
Size: 6674
BtgI C'CryG_G
Cuts at: 332 566 2261 2285 2597 2777 3191 3209 332
Size: 234 1695 24 312 180 414 18 3797
Fragments arranged by size:
3797 1695 414 312 234 180 24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4624 4805 4968 4624
Size: 181 163 6330
Fragments arranged by size:
6330 181 163
BtsI GCAGTG_nn'
Cuts at: 450 1242 1582 2321 2507 3917 450
Size: 792 340 739 186 1410 3207
Fragments arranged by size:
3207 1410 792 739 340 186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 840 875 840
Size: 35 6639
DraI TTT'AAA
Cuts at: 3673 3673
Size: 6674
DraIII CAC_nnn'GTG
Cuts at: 2682 2682
Size: 6674
DrdI GACnn_nn'nnGTC
Cuts at: 4380 5724 4380
Size: 1344 5330
EaeI y'GGCC_r
Cuts at: 731 1286 1469 2148 2415 2489 2584 3188 4259
Size: 555 183 679 267 74 95 604 1071
Cuts at: 4259 4433 4824 4851 6196 731
Size: 174 391 27 1345 1209
Fragments arranged by size:
1345 1209 1071 679 604 555 391 267
183 174 95 74 27
EagI C'GGCC_G
Cuts at: 2489 4259 2489
Size: 1770 4904
EarI CTCTTCn'nnn_
Cuts at: 895 946 1565 1750 1807 1966 2446 3358 4697
Size: 51 619 185 57 159 480 912 1339
Cuts at: 4697 4907 895
Size: 210 2662
Fragments arranged by size:
2662 1339 912 619 480 210 185 159
57 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 1614 3676 4882 5624 5770 6484 265
Size: 1349 2062 1206 742 146 714 455
Fragments arranged by size:
2062 1349 1206 742 714 455 146
EcoICRI GAG'CTC
Cuts at: 1401 2100 2541 3686 1401
Size: 699 441 1145 4389
Fragments arranged by size:
4389 1145 699 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 412 598 735 1072 1203 1228 1365 1567
Size: 199 186 137 337 131 25 137 202
Cuts at: 1567 1729 1741 1831 1915 2032 2095 2107 2236
Size: 162 12 90 84 117 63 12 129
Cuts at: 2236 2283 2299 2869 2881 2980 3028 3061 3145
Size: 47 16 570 12 99 48 33 84
Cuts at: 3145 3664 4499 4931 5284 5946 213
Size: 519 835 432 353 662 941
Fragments arranged by size:
941 835 662 570 519 432 353 337
202 199 186 162 137 137 131 129
117 99 90 84 84 63 48 47
33 25 16 12 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2298 2298
Size: 6674
EcoO109I rG'GnC_Cy
Cuts at: 294 338 432 534 571 597 681 738 1114
Size: 44 94 102 37 26 84 57 376
Cuts at: 1114 2574 2602 2646 2865 2964 3119 3308 3813
Size: 1460 28 44 219 99 155 189 505
Cuts at: 3813 294
Size: 3155
Fragments arranged by size:
3155 1460 505 376 219 189 155 102
99 94 84 57 44 44 37 28
26
EcoRI G'AATT_C
Cuts at: 3678 3678
Size: 6674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 951 4693 951
Size: 3742 2932
FspI TGC'GCA
Cuts at: 442 2548 4455 442
Size: 2106 1907 2661
Fragments arranged by size:
2661 2106 1907
HaeII r_GCGC'y
Cuts at: 314 831 1887 2130 2515 2905 4108 4356 5586
Size: 517 1056 243 385 390 1203 248 1230
Cuts at: 5586 6357 314
Size: 771 631
Fragments arranged by size:
1230 1203 1056 771 631 517 390 385
248 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 991 1023 2146 2178 2281 2313 3418 3450 4516
Size: 32 1123 32 103 32 1105 32 1066
Cuts at: 4516 4546 4548 4578 991
Size: 30 2 30 3087
Fragments arranged by size:
3087 1123 1105 1066 103 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 218 1191 3713 218
Size: 973 2522 3179
Fragments arranged by size:
3179 2522 973
KasI G'GCGC_C
Cuts at: 4104 4352 4104
Size: 248 6426
KpnI G_GTAC'C
Cuts at: 566 3694 566
Size: 3128 3546
MluI A'CGCG_T
Cuts at: 6107 6107
Size: 6674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1760 1773 5433 5617 1334
Size: 426 13 3660 184 2391
Fragments arranged by size:
3660 2391 426 184 13
MscI TGG'CCA
Cuts at: 733 2417 2586 4435 733
Size: 1684 169 1849 2972
Fragments arranged by size:
2972 1849 1684 169
MslI CAynn'nnrTG
Cuts at: 895 1133 3415 4663 4790 895
Size: 238 2282 1248 127 2779
Fragments arranged by size:
2779 2282 1248 238 127
MspA1I CmG'CkG
Cuts at: 136 972 1196 1388 1467 1650 1696 2010 2636
Size: 836 224 192 79 183 46 314 626
Cuts at: 2636 3183 3211 3785 4099 4459 5243 5488 136
Size: 547 28 574 314 360 784 245 1322
Fragments arranged by size:
1322 836 784 626 574 547 360 314
314 245 224 192 183 79 46 28
NaeI GCC'GGC
Cuts at: 3553 4855 3553
Size: 1302 5372
NarI GG'CG_CC
Cuts at: 4105 4353 4105
Size: 248 6426
NcoI C'CATG_G
Cuts at: 566 566
Size: 6674
NgoMIV G'CCGG_C
Cuts at: 3551 4853 3551
Size: 1302 5372
NspI r_CATG'y
Cuts at: 381 771 1557 3727 4227 5830 381
Size: 390 786 2170 500 1603 1225
Fragments arranged by size:
2170 1603 1225 786 500 390
PasI CC'CwG_GG
Cuts at: 298 298
Size: 6674
PciI A'CATG_T
Cuts at: 5826 5826
Size: 6674
PflMI CCAn_nnn'nTGG
Cuts at: 1388 1707 1388
Size: 319 6355
PfoI T'CCnGG_A
Cuts at: 1166 2348 1166
Size: 1182 5492
PmeI GTTT'AAAC
Cuts at: 3673 3673
Size: 6674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 5120
Size: 32 6642
PpuMI rG'GwC_Cy
Cuts at: 294 432 738 1114 3119 3308 294
Size: 138 306 376 2005 189 3660
Fragments arranged by size:
3660 2005 376 306 189 138
PshAI GACnn'nnGTC
Cuts at: 1856 1856
Size: 6674
PspOMI G'GGCC_C
Cuts at: 138 570 2602 3270 138
Size: 432 2032 668 3542
Fragments arranged by size:
3542 2032 668 432
PspXI vC'TCGA_Gb
Cuts at: 427 427
Size: 6674
PstI C_TGCA'G
Cuts at: 1649 1742 2900 3721 4406 1649
Size: 93 1158 821 685 3917
Fragments arranged by size:
3917 1158 821 685 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6674
PvuII CAG'CTG
Cuts at: 972 1388 1467 1650 2010 2636 4099 4459 972
Size: 416 79 183 360 626 1463 360 3187
Fragments arranged by size:
3187 1463 626 416 360 360 183 79
RsrII CG'GwC_CG
Cuts at: 4869 4869
Size: 6674
SacI G_AGCT'C
Cuts at: 1403 2102 2543 3688 1403
Size: 699 441 1145 4389
Fragments arranged by size:
4389 1145 699 441
SacII CC_GC'GG
Cuts at: 3212 3212
Size: 6674
SalI G'TCGA_C
Cuts at: 216 1189 3711 216
Size: 973 2522 3179
Fragments arranged by size:
3179 2522 973
SapI GCTCTTCn'nnn_
Cuts at: 895 1750 4697 4907 895
Size: 855 2947 210 2662
Fragments arranged by size:
2947 2662 855 210
SbfI CC_TGCA'GG
Cuts at: 3721 3721
Size: 6674
ScaI AGT'ACT
Cuts at: 6191 6191
Size: 6674
SexAI A'CCwGG_T
Cuts at: 578 1250 578
Size: 672 6002
SfcI C'TryA_G
Cuts at: 33 1524 1645 1738 1941 2896 2937 3717 4402
Size: 1491 121 93 203 955 41 780 685
Cuts at: 4402 5370 5561 33
Size: 968 191 1146
Fragments arranged by size:
1491 1146 968 955 780 685 203 191
121 93 41
SfoI GGC'GCC
Cuts at: 4106 4354 4106
Size: 248 6426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
SmaI CCC'GGG
Cuts at: 118 351 3696 118
Size: 233 3345 3096
Fragments arranged by size:
3345 3096 233
SmlI C'TyrA_G
Cuts at: 427 637 1258 3240 3634 3829 4744 5458 5720
Size: 210 621 1982 394 195 915 714 262
Cuts at: 5720 427
Size: 1381
Fragments arranged by size:
1982 1381 915 714 621 394 262 210
195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6674
SphI G_CATG'C
Cuts at: 771 3727 4227 771
Size: 2956 500 3218
Fragments arranged by size:
3218 2956 500
StuI AGG'CCT
Cuts at: 2566 2566
Size: 6674
StyI C'CwwG_G
Cuts at: 566 677 734 3471 3808 4162 566
Size: 111 57 2737 337 354 3078
Fragments arranged by size:
3078 2737 354 337 111 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 191 2028 2349 2422 2896 4257 4390 4849 5058
Size: 1837 321 73 474 1361 133 459 209
Cuts at: 5058 191
Size: 1807
Fragments arranged by size:
1837 1807 1361 474 459 321 209 133
73
TatI w'GTAC_w
Cuts at: 539 2730 6189 539
Size: 2191 3459 1024
Fragments arranged by size:
3459 2191 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 2854 2886 3052 3084 6191 6223 2296
Size: 32 526 32 166 32 3107 32 2747
Fragments arranged by size:
3107 2747 526 166 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 4471 1915
Size: 2556 4118
XbaI T'CTAG_A
Cuts at: 3705 3705
Size: 6674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 622 726 2070 4169 622
Size: 104 1344 2099 3127
Fragments arranged by size:
3127 2099 1344 104
XhoI C'TCGA_G
Cuts at: 427 427
Size: 6674
XmaI C'CCGG_G
Cuts at: 116 349 3694 116
Size: 233 3345 3096
Fragments arranged by size:
3345 3096 233
ZraI GAC'GTC
Cuts at: 1986 2922 1986
Size: 936 5738
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BmtI BsiWI BsmI BspEI
BssHII BstXI ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI